AT3G10840

Serine hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010989
Physical Location & Seq
Reverse (-)
3390153 .. 3393536
3384 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10840.3

Sequence Viewer

Length: 1401 bp
ATGAATTTAAACACCACATGCATCTTCTTTTTGAACCAACCAAAGCAGACTCTCTCAACATTATCTCTCAGCGACAAGTTTAGTTCAGATTCCTCTAGAACCCATTTTCAGATGTCTCGTCAGATTTCCGGCCAACCCTCTCGCCGTCTCATCGTCTCCAATGCTGCTTCTTCTCCTTCTCTCTCTGGTGGCTTCCCAGATGCTGGAGCACATAGTAAAGAGGAGATGGAAGCTGAAACAGAACAGGATCCAATGAACCTAGCTGATCCAGACAGTTGTTTCTGTGAGTTTCAAGGTGTTCATATACACCACAAAGTGTTAGATCCACATACACTCTCTGATGATGTTTCAAACACAAGCCCTCATGCTCAAGAAACACCAAAAACCAAGTTTCCGATGATTCTATTGCACGGTTTCGGCGCTTCTGTGTTCTCCTGGAACAGAGTTATGAAGCCATTAGCTCGTCTCGTTAGTTCAAAAGTTCTTGCCTTTGATAGACCAGCATTTGGTTTGACTTCTCGGATTTTTCACCCGTTTAGTGGTGCTACCAATGATGCAAAACCATTGAATCCCTACTCCATGGTGTATTCCGTGCTCACTACGTTGTATTTCATCGATGTTCTTGCTGCCGATAAGGCGATTCTTGTGGGGCATTCTGCTGGTTGCCCTGTAGCTTTAGATGCTTACTTTGAAGCCCCGGAGCGTGTTGCTGCTCTCATTCTCGTCGCTCCAGCTATATTCGCTCCCCGTCCTGTGGCCACAACTGATGCTGGAGAGAACCGTGACAAAGAAGCTCCCACTAGCAACTTTCTAGGGACTCTAGTTGAGTTAACCAAAGGTGTTATAAGAGCAGTATTGAGAGTGGTAACGGGAATGGCGAATATGCTGAGTTCTCTCTACAAGAAAGCTTTAGCAGCTTTCCTCAGATCCTTCCTTGGAGTAATGCTGGTGAGGATGGCAATCAATAAATTTGGAGTAACAGCTGTTAGGAACGCGTGGTATGACTCAAAACAAGTGACGGATCATGTCGTGCAGGGTTATACAAAGCCTCTTAAAGCCAAGGGATGGGACAAAGCTCTCGTGGAATTCACGGTGGCTACACTAACAGATAATAATGGCTCAGAGAAAAAACTCCCATTGTCTAAAAGGCTTCAAGAAATCAAATGCCCTGTTCTGATTGTGACGGGAGATACGGACCGTATTGTGCCAGCATGGAACGCAGAGAGGCTGGCACGAGCCATACCGGGATCAGTGTTCGAAGTAATTAAGAAATGTGGTCATCTTCCTCAAGAGGAAAAACCAGATGAATTCATATCTATTGTTGCAAAGTTTCTAGGGAATGCTTTTGGAGGGTCACAACAACAAGACGTGGACTTGAAGTTCCAAGGCATTGTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

466

Amino Acids

50.88

Weight (kDa)

8.59

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 71 - 373 1.5e-21 alpha/beta hydrolase fold
Hydrolase_4 PF12146 72 - 369 9.1e-09 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 73 - 377 2.2e-23 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10840 AT3G10840 AT3G10840 AT3G10840
fragaria_vesca FvH4_7g26310
malus_domestica MD07G1238400.v1.1
prunus_persica Prupe.2G267200_v2.0.a1 Prupe.2G267200_v2.0.a1
pyrus_communis pycom07g21440
rosa_chinensis RchiOBHm_Chr1g0372761
rosa_laevigata RLG00000026874
rosa_multiflora Rmu_co8126034.1_g000001 Rmu_sc0009717.1_g000006
rosa_roxburghii Rroxscaffold_4G00284700
rosa_rugosa Rorug01G0374200
rosa_samantha Rh1AG382900 Rh1BG346500 Rh1CG359700 Rh1DG377500
rosa_wichuraiana Rw1G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 845
AccB7I CCANNNNNTGG 3 cut(s) 203, 506, 1065
AccII CGCG 1 cut(s) 995
AclWI GGATC 7 cut(s) 242, 255, 260, 317, 921, 1029, 1255
AcoI YGGCCR 2 cut(s) 130, 756
AcsI RAATTY 4 cut(s) 4, 968, 1085, 1307
AdeI CACNNNGTG 1 cut(s) 316
AfiI CCNNNNNNNGG 5 cut(s) 203, 506, 539, 754, 1065
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 8 cut(s) 34, 293, 351, 477, 568, 692, 1154, 1378
AjiI CACGTC 1 cut(s) 1369
AjnI CCWGG 1 cut(s) 434
AloI GAACNNNNNNTCC 2 cut(s) 1364, 1396
Alw21I GWGCWC 2 cut(s) 211, 597
Alw26I GTCTC 4 cut(s) 120, 152, 160, 470
AlwI GGATC 7 cut(s) 242, 255, 260, 317, 921, 1029, 1255
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 2 cut(s) 130, 756
ApeKI GCWGC 4 cut(s) 164, 626, 710, 914
ApoI RAATTY 4 cut(s) 4, 968, 1085, 1307
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 1195
AsuC2I CCSGG 2 cut(s) 698, 1245
AsuHPI GGTGA 2 cut(s) 521, 961
AsuII TTCGAA 1 cut(s) 1257
AvaII GGWCC 1 cut(s) 1195
BalI TGGCCA 1 cut(s) 758
BamHI GGATCC 1 cut(s) 247
BauI CACGAG 2 cut(s) 1079, 1233
Bbv12I GWGCWC 2 cut(s) 211, 597
BbvI GCAGC 4 cut(s) 151, 613, 697, 926
BccI CCATC 3 cut(s) 220, 949, 1059
BceAI ACGGC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 1 cut(s) 436
BcnI CCSGG 2 cut(s) 698, 1245
BcoDI GTCTC 4 cut(s) 120, 152, 160, 470
BfaI CTAG 6 cut(s) 96, 260, 801, 812, 821, 1334
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 423
BglI GCCNNNNNGGC 1 cut(s) 635
BisI GCNGC 4 cut(s) 165, 627, 711, 915
BlsI GCNGC 4 cut(s) 166, 628, 712, 916
Bme1390I CCNGG 3 cut(s) 436, 698, 1245
Bme18I GGWCC 1 cut(s) 1195
BmgBI CACGTC 1 cut(s) 1369
BmgT120I GGNCC 1 cut(s) 1195
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 3 cut(s) 436, 698, 1245
BmsI GCATC 5 cut(s) 30, 190, 544, 670, 757
BpmI CTGGAG 3 cut(s) 225, 714, 792
Bpu14I TTCGAA 1 cut(s) 1257
BpuEI CTTGAG 2 cut(s) 354, 1272
BpuMI CCSGG 2 cut(s) 698, 1245
Bsa29I ATCGAT 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 959
BsaJI CCNNGG 5 cut(s) 579, 696, 934, 1059, 1384
Bsc4I CCNNNNNNNGG 5 cut(s) 203, 506, 539, 754, 1065
Bse8I GATNNNNATC 1 cut(s) 959
BseBI CCWGG 1 cut(s) 436
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 5 cut(s) 579, 696, 934, 1059, 1384
BseGI GGATG 2 cut(s) 960, 1070
BseJI GATNNNNATC 1 cut(s) 959
BseLI CCNNNNNNNGG 5 cut(s) 203, 506, 539, 754, 1065
BseMII CTCAG 4 cut(s) 82, 878, 937, 1134
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 4 cut(s) 151, 613, 697, 926
BsgI GTGCAG 1 cut(s) 1052
Bsh1236I CGCG 1 cut(s) 995
BshFI GGCC 2 cut(s) 132, 758
BshVI ATCGAT 1 cut(s) 615
BsiHKAI GWGCWC 2 cut(s) 211, 597
BsiSI CCGG 3 cut(s) 129, 698, 1244
BslFI GGGAC 2 cut(s) 829, 1082
BslI CCNNNNNNNGG 5 cut(s) 203, 506, 539, 754, 1065
BsmAI GTCTC 4 cut(s) 120, 152, 160, 470
BsmBI CGTCTC 3 cut(s) 152, 160, 470
BsmFI GGGAC 2 cut(s) 829, 1082
BsmI GAATGC 2 cut(s) 652, 1345
BsnI GGCC 2 cut(s) 132, 758
Bsp119I TTCGAA 1 cut(s) 1257
Bsp1286I GDGCHC 2 cut(s) 211, 597
Bsp143I GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
Bsp19I CCATGG 1 cut(s) 579
BspANI GGCC 2 cut(s) 132, 758
BspCNI CTCAG 4 cut(s) 81, 879, 936, 1133
BspDI ATCGAT 1 cut(s) 615
BspFNI CGCG 1 cut(s) 995
BspHI TCATGA 1 cut(s) 1397
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 7 cut(s) 242, 255, 260, 317, 921, 1029, 1255
BspT104I TTCGAA 1 cut(s) 1257
BssECI CCNNGG 5 cut(s) 579, 696, 934, 1059, 1384
BssMI GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
BssSI CACGAG 2 cut(s) 1079, 1233
BssT1I CCWWGG 4 cut(s) 579, 934, 1059, 1384
Bst2BI CACGAG 2 cut(s) 1079, 1233
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 5 cut(s) 275, 413, 782, 1093, 1199
BstBI TTCGAA 1 cut(s) 1257
BstC8I GCNNGC 2 cut(s) 1209, 1230
BstDEI CTNAG 4 cut(s) 68, 887, 923, 1120
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 2 cut(s) 960, 1070
BstFNI CGCG 1 cut(s) 995
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 6 cut(s) 250, 268, 325, 929, 1024, 1250
BstMAI GTCTC 4 cut(s) 120, 152, 160, 470
BstMBI GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
BstMWI GCNNNNNNNGC 5 cut(s) 635, 680, 740, 914, 1217
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 1 cut(s) 21
BstSCI CCNGG 3 cut(s) 434, 696, 1243
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 1 cut(s) 995
BstV1I GCAGC 4 cut(s) 151, 613, 697, 926
BstX2I RGATCY 3 cut(s) 247, 322, 926
BstYI RGATCY 3 cut(s) 247, 322, 926
Bsu15I ATCGAT 1 cut(s) 615
BsuRI GGCC 2 cut(s) 132, 758
BsuTUI ATCGAT 1 cut(s) 615
BtgI CCRYGG 1 cut(s) 579
BtrI CACGTC 1 cut(s) 1369
BtsCI GGATG 2 cut(s) 960, 1070
BtsIMutI CAGTG 1 cut(s) 1257
Cac8I GCNNGC 2 cut(s) 1209, 1230
CaiI CAGNNNCTG 1 cut(s) 203
CciI TCATGA 1 cut(s) 1397
CfoI GCGC 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 1195
ClaI ATCGAT 1 cut(s) 615
CpoI CGGWCCG 1 cut(s) 1195
CspI CGGWCCG 1 cut(s) 1195
CviAII CATG 6 cut(s) 18, 365, 580, 1025, 1212, 1398
DdeI CTNAG 4 cut(s) 68, 887, 923, 1120
DpnI GATC 6 cut(s) 249, 267, 324, 928, 1023, 1249
DpnII GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
DraI TTTAAA 1 cut(s) 9
DraIII CACNNNGTG 1 cut(s) 316
EaeI YGGCCR 2 cut(s) 130, 756
Eco130I CCWWGG 4 cut(s) 579, 934, 1059, 1384
Eco47I GGWCC 1 cut(s) 1195
EcoRI GAATTC 2 cut(s) 1085, 1307
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 4 cut(s) 579, 934, 1059, 1384
EcoT22I ATGCAT 1 cut(s) 23
ErhI CCWWGG 4 cut(s) 579, 934, 1059, 1384
Esp3I CGTCTC 3 cut(s) 152, 160, 470
FaeI CATG 6 cut(s) 21, 368, 583, 1028, 1215, 1401
FaqI GGGAC 2 cut(s) 829, 1082
FatI CATG 6 cut(s) 17, 364, 579, 1024, 1211, 1397
Fnu4HI GCNGC 4 cut(s) 165, 627, 711, 915
FokI GGATG 2 cut(s) 967, 1077
Fsp4HI GCNGC 4 cut(s) 165, 627, 711, 915
FspBI CTAG 6 cut(s) 96, 260, 801, 812, 821, 1334
GlaI GCGC 1 cut(s) 421
GluI GCNGC 4 cut(s) 165, 627, 711, 915
GsuI CTGGAG 3 cut(s) 225, 714, 792
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 2 cut(s) 132, 758
HapII CCGG 3 cut(s) 129, 698, 1244
HhaI GCGC 1 cut(s) 422
Hin1II CATG 6 cut(s) 21, 368, 583, 1028, 1215, 1401
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HincII GTYRAC 1 cut(s) 831
HindII GTYRAC 1 cut(s) 831
HindIII AAGCTT 1 cut(s) 906
HinfI GANTC 7 cut(s) 49, 89, 400, 568, 640, 817, 1004
HpaI GTTAAC 1 cut(s) 831
HpaII CCGG 3 cut(s) 129, 698, 1244
HphI GGTGA 2 cut(s) 521, 961
Hpy166II GTNNAC 2 cut(s) 831, 1372
Hpy188I TCNGA 9 cut(s) 88, 111, 123, 340, 396, 522, 926, 1123, 1176
Hpy188III TCNNGA 6 cut(s) 96, 269, 371, 1154, 1289, 1398
Hpy8I GTNNAC 2 cut(s) 831, 1372
Hpy99I CGWCG 1 cut(s) 728
HpyAV CCTTC 2 cut(s) 186, 940
HpyCH4III ACNGT 5 cut(s) 275, 413, 782, 1093, 1199
HpyCH4IV ACGT 2 cut(s) 602, 1368
HpyCH4V TGCA 5 cut(s) 21, 409, 557, 1033, 1325
HpyF10VI GCNNNNNNNGC 5 cut(s) 635, 680, 740, 914, 1217
HpyF3I CTNAG 4 cut(s) 68, 887, 923, 1120
HpySE526I ACGT 2 cut(s) 602, 1368
Hsp92II CATG 6 cut(s) 21, 368, 583, 1028, 1215, 1401
HspAI GCGC 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 831
Kzo9I GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
LmnI GCTCC 5 cut(s) 206, 700, 733, 748, 799
Lsp1109I GCAGC 4 cut(s) 151, 613, 697, 926
LweI GCATC 5 cut(s) 30, 190, 544, 670, 757
MaeI CTAG 6 cut(s) 96, 260, 801, 812, 821, 1334
MaeII ACGT 2 cut(s) 602, 1368
MaeIII GTNAC 6 cut(s) 782, 865, 976, 1015, 1180, 1353
MalI GATC 6 cut(s) 249, 267, 324, 928, 1023, 1249
MboI GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
MboII GAAGA 3 cut(s) 16, 162, 1274
MflI RGATCY 3 cut(s) 247, 322, 926
MhlI GDGCHC 2 cut(s) 211, 597
MlsI TGGCCA 1 cut(s) 758
MluCI AATT 5 cut(s) 4, 968, 1085, 1263, 1307
MluI ACGCGT 1 cut(s) 993
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 3 cut(s) 43, 811, 998
Mox20I TGGCCA 1 cut(s) 758
Mph1103I ATGCAT 1 cut(s) 23
MscI TGGCCA 1 cut(s) 758
MseI TTAA 4 cut(s) 8, 830, 1053, 1266
Msp20I TGGCCA 1 cut(s) 758
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 3 cut(s) 129, 698, 1244
MspR9I CCNGG 3 cut(s) 436, 698, 1245
Mva1269I GAATGC 2 cut(s) 652, 1345
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 1 cut(s) 995
MwoI GCNNNNNNNGC 5 cut(s) 635, 680, 740, 914, 1217
NciI CCSGG 2 cut(s) 698, 1245
NcoI CCATGG 1 cut(s) 579
NdeII GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
NlaIII CATG 6 cut(s) 21, 368, 583, 1028, 1215, 1401
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 4 cut(s) 782, 1015, 1180, 1353
NsiI ATGCAT 1 cut(s) 23
NspI RCATGY 1 cut(s) 21
NspV TTCGAA 1 cut(s) 1257
PagI TCATGA 1 cut(s) 1397
PcsI WCGNNNNNNNCGW 1 cut(s) 875
PctI GAATGC 2 cut(s) 652, 1345
PfeI GAWTC 4 cut(s) 89, 400, 568, 640
PflMI CCANNNNNTGG 3 cut(s) 203, 506, 1065
PfoI TCCNGGA 1 cut(s) 434
PkrI GCNGC 4 cut(s) 166, 628, 712, 916
PleI GAGTC 3 cut(s) 43, 811, 998
PpsI GAGTC 3 cut(s) 43, 811, 998
PsiI TTATAA 1 cut(s) 845
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 1195
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 3 cut(s) 247, 322, 926
PvuII CAGCTG 1 cut(s) 983
Rsr2I CGGWCCG 1 cut(s) 1195
RsrII CGGWCCG 1 cut(s) 1195
SaqAI TTAA 4 cut(s) 8, 830, 1053, 1266
SatI GCNGC 4 cut(s) 165, 627, 711, 915
Sau3AI GATC 6 cut(s) 247, 265, 322, 926, 1021, 1247
Sau96I GGNCC 1 cut(s) 1195
SchI GAGTC 3 cut(s) 43, 811, 998
ScrFI CCNGG 3 cut(s) 436, 698, 1245
SduI GDGCHC 2 cut(s) 211, 597
SfaNI GCATC 5 cut(s) 30, 190, 544, 670, 757
SfcI CTRYAG 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 1257
SinI GGWCC 1 cut(s) 1195
SmlI CTYRAG 2 cut(s) 369, 1287
SmoI CTYRAG 2 cut(s) 369, 1287
Sse9I AATT 5 cut(s) 4, 968, 1085, 1263, 1307
SspMI CTAG 6 cut(s) 96, 260, 801, 812, 821, 1334
StyD4I CCNGG 3 cut(s) 434, 696, 1243
StyI CCWWGG 4 cut(s) 579, 934, 1059, 1384
TaaI ACNGT 5 cut(s) 275, 413, 782, 1093, 1199
TaiI ACGT 2 cut(s) 605, 1371
TaqI TCGA 2 cut(s) 615, 1257
TasI AATT 5 cut(s) 4, 968, 1085, 1263, 1307
TfiI GAWTC 4 cut(s) 89, 400, 568, 640
Tru1I TTAA 4 cut(s) 8, 830, 1053, 1266
Tru9I TTAA 4 cut(s) 8, 830, 1053, 1266
TscAI CASTG 1 cut(s) 1257
TseFI GTSAC 4 cut(s) 782, 1015, 1180, 1353
TseI GCWGC 4 cut(s) 164, 626, 710, 914
Tsp45I GTSAC 4 cut(s) 782, 1015, 1180, 1353
TspDTI ATGAA 7 cut(s) 17, 269, 290, 464, 601, 1300, 1320
TspGWI ACGGA 3 cut(s) 580, 1034, 1208
TspRI CASTG 1 cut(s) 1257
Van91I CCANNNNNTGG 3 cut(s) 203, 506, 1065
VpaK11BI GGWCC 1 cut(s) 1195
XapI RAATTY 4 cut(s) 4, 968, 1085, 1307
XbaI TCTAGA 1 cut(s) 95
XceI RCATGY 1 cut(s) 21
XspI CTAG 6 cut(s) 96, 260, 801, 812, 821, 1334
Zsp2I ATGCAT 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.