MD07G1238400.v1.1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010989
Physical Location & Seq
Forward (+)
31034896 .. 31037641
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1238400.v1.1.491

Sequence Viewer

Length: 984 bp
ATGAAGCCATTGGCAGAGGCTACTGGTTCCAAGGTTCTTGCCTTTGATAGACCAGCCTTTGGATTGACATCAAGGGTAAGCGCTTTTGGGCATTCAGCTTCTGAAAATGGGGAATCCAAACCTTTGAATCCATATTCCATGGCATTTGCAGTGCTTGCTACCTTGTACTTCATTGATTTTCTGGCAGCTGAGAAGGCGATTCTGGTGGGGCATTCCGCTGGTTGTCTTGTAGCAGTTGATTCATATTTTGAATCCCCAGAGCGTGTTGCTGCTCTAATCCTTATCTCCCCAGCAATTTTTGCCCCAAGGGCTATTGAGAAGGTTGCTAAGGGCCATCAACCGGAAGAAGATAACCAGACTGAAGAAGATGCCTCAAATTCTGTAAATCAAGGAAATCCATTTATCCAGTTTTTCAAGACTCTGTCCAAGTTTACCAAATTTATTTCAGATGCAGTAATGCGACTCGTGAAGGGAATGGTTGGCATGCTTAACTCTTTGTACAAGAAATTCTTATTGGCTATTCTACGCTCTGCCTTTGCTGCAATGCTTGTGAGGATGGCTATTGATAAATTTGGTGTTGCTGGTGTTAAGAATGCATGGTATGACTCGAAACAAGTCACCGAACATGTCATACAAGGTTATACAAAGCCATTGAGGATTAAGGGTTGGGACAAGGCACTTCTGGAGTACACAGCAGCAATGCTCACAGACACTTCATCTAAGTCGAAGCCACCATTGTCGAAAAGACTTTGTGAAATCTCATGCCCTGTTCTGATAATTACGGGTGATACTGACCGGATTGTCCCCTCGTGGAATGCCAAGAGGCTATCACAAGCCATACCCGGGTCTTACCTTGAAGTAATCAAGCATTGTGGGCACTTGCCACATGAAGAGAAATTTCATGAGTTTGTCTCAATAGTTAAGAAGTTTCTGCAAACAGCTTTGGGAGATTCTCAGGAGCAGCACATACAAGCTGTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

328

Amino Acids

35.95

Weight (kDa)

8.87

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 3 - 299 4.3e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 3 - 304 9.4e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10840 AT3G10840 AT3G10840 AT3G10840
fragaria_vesca FvH4_7g26310
malus_domestica MD07G1238400.v1.1
prunus_persica Prupe.2G267200_v2.0.a1 Prupe.2G267200_v2.0.a1
pyrus_communis pycom07g21440
rosa_chinensis RchiOBHm_Chr1g0372761
rosa_laevigata RLG00000026874
rosa_multiflora Rmu_co8126034.1_g000001 Rmu_sc0009717.1_g000006
rosa_roxburghii Rroxscaffold_4G00284700
rosa_rugosa Rorug01G0374200
rosa_samantha Rh1AG382900 Rh1BG346500 Rh1CG359700 Rh1DG377500
rosa_wichuraiana Rw1G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 800
AccB7I CCANNNNNTGG 1 cut(s) 59
AciI CCGC 1 cut(s) 216
AcsI RAATTY 5 cut(s) 376, 437, 506, 569, 896
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 3 cut(s) 167, 500, 689
AfeI AGCGCT 1 cut(s) 82
AfiI CCNNNNNNNGG 3 cut(s) 59, 340, 843
AflIII ACRYGT 1 cut(s) 625
AgsI TTSAA 4 cut(s) 127, 251, 415, 857
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 4 cut(s) 98, 188, 941, 974
AluI AGCT 4 cut(s) 98, 188, 941, 974
Alw26I GTCTC 1 cut(s) 916
AlwNI CAGNNNCTG 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 842
Aor51HI AGCGCT 1 cut(s) 82
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 5 cut(s) 185, 269, 539, 695, 961
ApoI RAATTY 5 cut(s) 376, 437, 506, 569, 896
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 331
AsuC2I CCSGG 2 cut(s) 843, 844
AsuHPI GGTGA 2 cut(s) 610, 797
AvaI CYCGRG 1 cut(s) 842
BaeGI GKGCMC 1 cut(s) 879
BauI CACGAG 2 cut(s) 464, 808
BbvI GCAGC 5 cut(s) 197, 256, 526, 707, 973
BccI CCATC 2 cut(s) 342, 550
BcnI CCSGG 2 cut(s) 843, 844
BcoDI GTCTC 1 cut(s) 916
BfmI CTRYAG 1 cut(s) 975
BfoI RGCGCY 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 5 cut(s) 186, 270, 540, 696, 962
BlsI GCNGC 5 cut(s) 187, 271, 541, 697, 963
Bme1390I CCNGG 2 cut(s) 843, 844
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 2 cut(s) 843, 844
BmsI GCATC 2 cut(s) 358, 439
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 327
BpuMI CCSGG 2 cut(s) 843, 844
BsaBI GATNNNNATC 1 cut(s) 67
BsaJI CCNNGG 4 cut(s) 30, 138, 305, 842
BsaWI WCCGGW 2 cut(s) 340, 795
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 340, 843
Bse1I ACTGG 2 cut(s) 28, 406
Bse3DI GCAATG 2 cut(s) 549, 705
Bse8I GATNNNNATC 1 cut(s) 67
BseDI CCNNGG 4 cut(s) 30, 138, 305, 842
BseGI GGATG 1 cut(s) 561
BseJI GATNNNNATC 1 cut(s) 67
BseLI CCNNNNNNNGG 3 cut(s) 59, 340, 843
BseMI GCAATG 2 cut(s) 549, 705
BseMII CTCAG 2 cut(s) 180, 968
BseNI ACTGG 2 cut(s) 28, 406
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 5 cut(s) 197, 256, 526, 707, 973
BseYI CCCAGC 1 cut(s) 289
BshFI GGCC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 3 cut(s) 341, 796, 843
BslFI GGGAC 2 cut(s) 683, 788
BslI CCNNNNNNNGG 3 cut(s) 59, 340, 843
BsmAI GTCTC 1 cut(s) 916
BsmFI GGGAC 2 cut(s) 683, 788
BsmI GAATGC 4 cut(s) 91, 211, 598, 820
BsnI GGCC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 842
Bsp1286I GDGCHC 1 cut(s) 879
Bsp1407I TGTACA 1 cut(s) 498
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 1 cut(s) 216
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 2 cut(s) 181, 967
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 1 cut(s) 28
BsrDI GCAATG 2 cut(s) 549, 705
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 28, 406
BssECI CCNNGG 4 cut(s) 30, 138, 305, 842
BssSI CACGAG 2 cut(s) 464, 808
BssT1I CCWWGG 3 cut(s) 30, 138, 305
Bst2BI CACGAG 2 cut(s) 464, 808
Bst6I CTCTTC 1 cut(s) 885
BstAPI GCANNNNNTGC 2 cut(s) 155, 299
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 156, 485
BstDEI CTNAG 4 cut(s) 189, 327, 720, 954
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 561
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstMAI GTCTC 1 cut(s) 916
BstMWI GCNNNNNNNGC 6 cut(s) 155, 194, 299, 308, 539, 874
BstNSI RCATGY 2 cut(s) 487, 629
BstSCI CCNGG 2 cut(s) 841, 842
BstSFI CTRYAG 1 cut(s) 975
BstSLI GKGCMC 1 cut(s) 879
BstV1I GCAGC 5 cut(s) 197, 256, 526, 707, 973
BsuRI GGCC 1 cut(s) 333
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 1 cut(s) 561
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 156, 485
CaiI CAGNNNCTG 1 cut(s) 101
CciI TCATGA 1 cut(s) 901
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 331
Cfr9I CCCGGG 1 cut(s) 842
Csp6I GTAC 3 cut(s) 166, 499, 688
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviAII CATG 7 cut(s) 139, 484, 597, 626, 762, 887, 902
CviQI GTAC 3 cut(s) 166, 499, 688
DdeI CTNAG 4 cut(s) 189, 327, 720, 954
DrdI GACNNNNNNGTC 1 cut(s) 800
DseDI GACNNNNNNGTC 1 cut(s) 800
Eam1104I CTCTTC 1 cut(s) 885
EarI CTCTTC 1 cut(s) 885
Eco130I CCWWGG 3 cut(s) 30, 138, 305
Eco47III AGCGCT 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 842
EcoT14I CCWWGG 3 cut(s) 30, 138, 305
EcoT22I ATGCAT 1 cut(s) 598
ErhI CCWWGG 3 cut(s) 30, 138, 305
FaeI CATG 7 cut(s) 142, 487, 600, 629, 765, 890, 905
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 2 cut(s) 683, 788
FatI CATG 7 cut(s) 138, 483, 596, 625, 761, 886, 901
Fnu4HI GCNGC 5 cut(s) 186, 270, 540, 696, 962
FokI GGATG 1 cut(s) 568
Fsp4HI GCNGC 5 cut(s) 186, 270, 540, 696, 962
GlaI GCGC 1 cut(s) 82
GluI GCNGC 5 cut(s) 186, 270, 540, 696, 962
GsaI CCCAGC 1 cut(s) 293
GsuI CTGGAG 1 cut(s) 704
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 333
HapII CCGG 3 cut(s) 341, 796, 843
HhaI GCGC 1 cut(s) 83
Hin1II CATG 7 cut(s) 142, 487, 600, 629, 765, 890, 905
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 9 cut(s) 113, 127, 199, 239, 251, 418, 462, 605, 950
HpaII CCGG 3 cut(s) 341, 796, 843
HphI GGTGA 2 cut(s) 610, 797
Hpy166II GTNNAC 2 cut(s) 432, 690
Hpy188I TCNGA 3 cut(s) 103, 448, 774
Hpy188III TCNNGA 5 cut(s) 415, 466, 683, 902, 956
Hpy8I GTNNAC 2 cut(s) 432, 690
HpyAV CCTTC 3 cut(s) 187, 313, 463
HpyCH4V TGCA 5 cut(s) 149, 452, 542, 596, 934
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 194, 299, 308, 539, 874
HpyF3I CTNAG 4 cut(s) 189, 327, 720, 954
Hsp92II CATG 7 cut(s) 142, 487, 600, 629, 765, 890, 905
HspAI GCGC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 958
Lsp1109I GCAGC 5 cut(s) 197, 256, 526, 707, 973
LweI GCATC 2 cut(s) 358, 439
MaeIII GTNAC 1 cut(s) 616
MboII GAAGA 5 cut(s) 356, 359, 374, 377, 902
MhlI GDGCHC 1 cut(s) 879
MluCI AATT 7 cut(s) 294, 376, 437, 506, 569, 777, 896
MlyI GAGTC 3 cut(s) 412, 456, 599
MnlI CCTC 6 cut(s) 10, 382, 546, 648, 816, 817
Mph1103I ATGCAT 1 cut(s) 598
MseI TTAA 4 cut(s) 489, 588, 660, 921
MspA1I CMGCKG 2 cut(s) 188, 218
MspI CCGG 3 cut(s) 341, 796, 843
MspR9I CCNGG 2 cut(s) 843, 844
Mva1269I GAATGC 4 cut(s) 91, 211, 598, 820
MwoI GCNNNNNNNGC 6 cut(s) 155, 194, 299, 308, 539, 874
NciI CCSGG 2 cut(s) 843, 844
NcoI CCATGG 1 cut(s) 138
NlaIII CATG 7 cut(s) 142, 487, 600, 629, 765, 890, 905
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 2 cut(s) 487, 629
PaeI GCATGC 1 cut(s) 487
PagI TCATGA 1 cut(s) 901
PciI ACATGT 1 cut(s) 625
PctI GAATGC 4 cut(s) 91, 211, 598, 820
PfeI GAWTC 6 cut(s) 113, 127, 199, 239, 251, 950
PflFI GACNNNGTC 1 cut(s) 421
PflMI CCANNNNNTGG 1 cut(s) 59
PkrI GCNGC 5 cut(s) 187, 271, 541, 697, 963
PleI GAGTC 3 cut(s) 412, 456, 599
PpsI GAGTC 3 cut(s) 412, 456, 599
PscI ACATGT 1 cut(s) 625
PspFI CCCAGC 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 1 cut(s) 331
PsrI GAACNNNNNNTAC 2 cut(s) 615, 647
PstNI CAGNNNCTG 1 cut(s) 101
PsyI GACNNNGTC 1 cut(s) 421
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 3 cut(s) 167, 500, 689
RsaNI GTAC 3 cut(s) 166, 499, 688
SaqAI TTAA 4 cut(s) 489, 588, 660, 921
SatI GCNGC 5 cut(s) 186, 270, 540, 696, 962
Sau96I GGNCC 1 cut(s) 331
SchI GAGTC 3 cut(s) 412, 456, 599
ScrFI CCNGG 2 cut(s) 843, 844
SduI GDGCHC 1 cut(s) 879
SfaNI GCATC 2 cut(s) 358, 439
SfcI CTRYAG 1 cut(s) 975
SmaI CCCGGG 1 cut(s) 844
SphI GCATGC 1 cut(s) 487
Sse9I AATT 7 cut(s) 294, 376, 437, 506, 569, 777, 896
SsiI CCGC 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 841, 842
StyI CCWWGG 3 cut(s) 30, 138, 305
TaqI TCGA 3 cut(s) 608, 725, 740
TasI AATT 7 cut(s) 294, 376, 437, 506, 569, 777, 896
TatI WGTACW 3 cut(s) 165, 498, 687
TfiI GAWTC 6 cut(s) 113, 127, 199, 239, 251, 950
Tru1I TTAA 4 cut(s) 489, 588, 660, 921
Tru9I TTAA 4 cut(s) 489, 588, 660, 921
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 5 cut(s) 185, 269, 539, 695, 961
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 6 cut(s) 17, 160, 231, 705, 890, 903
TspMI CCCGGG 1 cut(s) 842
TspRI CASTG 1 cut(s) 156
Tth111I GACNNNGTC 1 cut(s) 421
Van91I CCANNNNNTGG 1 cut(s) 59
XapI RAATTY 5 cut(s) 376, 437, 506, 569, 896
XceI RCATGY 2 cut(s) 487, 629
XmaI CCCGGG 1 cut(s) 842
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.