RLG00000026874

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010989
Physical Location & Seq
Reverse (-)
6705095 .. 6707733
2639 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026874

Sequence Viewer

Length: 1452 bp
ATGAGGAGCTTGGCGGGGATATGGGTGTTGCCGAGTTGCAGTAAAGTTTCGAGCTTTAATGGCAGACTCATTAGCTCAAGCTTTGCTTCGAGATTTGATGGCCCAGATACTCCAAACTACGTCGTTTTGAAGTCTTCTCCGGTGGCTGCTGCTGCTTCAGTCAGCGGCTCCGGTGCCCAGTACTCCGGTTCTGGAGGATTGCTTGGTGTGCAAACAAAGGAGAAGAAGAAGAAGAAGGAGGAAATAGCTGGTATTGATCAAGATGAACTGGTGGAGGATCCAAAGCTTTTAGCTGACCCAGATAGTTGTTTTTGTGAGTTTAGTGGAGTGGAGATCCATCATACGGTGTATGAAGCACAATTAAAGGTACATGAGACTGAGAGTGTCTCAAGTGAGACTAAGACTGTCGCTCTTGCTATGATCTTGTTACATGGGTTTGGAGCCTCGGTTTTCTCTTGGAAACGGGTTATGAAGCCGTTGGCCGAGGCCATTGGTTCCAAAGCTCTGGCGTTTGATAGGCCGGCGTTTGGGTTGACATCTAGGGGAAACTCTGTCAGGCATTTATCTTCTGGAAATGGGGAATCCAAACCTGTGAATCCGTATTCCATGGCATTTTCAGTGCTTGCAACCTTGTATTTCATTGATTTTCTGGCAGCTGAGAAGGCCATTATAGTGGGGCATTCAGCTGGTTCTCTTGTAGCAGTGGAGTCATATTTTGAAGCACCAGAACGTGTGGCCGCTCTAATCCTTGTTGCCCCAGCCATTTTTGCCCCAATTACAACCCAAAAGGTTGACAAGGGATCTCAGCCTGGAACAAATAACCAGACAGAAGAAGATGGCTCAAATTCAGTTGATAATGGGAATCCATTTGTCCAGTTCTCTAAGGTTCTGTCCAAATTTGCCAGATTTATTTCACAGGCAATTATGGGATTGGTGAAAGGGATGGCAGTTATGCTCAACTCTTTGTATAAGAAATTCTTGTCAGCTCTTCTGCGCTCTGCTTTTGCGATAATGCTGGTAAGAATGGCAATCAATAAATTCGGTATCGCTGCTGTTAGGAATGCATGGTATGACTCGAAGAAAGTCACTGATGAAGACATCAGTGGTTATACAAAGCCATTGAGGACGAAGGGTTGGGATAAGGCACTTGTGGAGTACACGGCAGCAATGCTCACAGACACTTCATCTGAATCAAAGCCACCATTGGCAAAGAGACTCCGTGATATCAAATGTCCTGTTCTGATTGTTACGGGTGATACTGACCGAATTGTCCCGTCATGGAATGCGAAGAGACTTTCAAAAGCCATACCGGGGTCTTGCTTTGAAGTAATAAAGCGTTGTGGGCACTTGCCACATGAAGAGAAAGTTGACGAGTTTGTTTCAATAGTTAAGAAATTTCTACAAACCGCTTTTGATGATTCTCTGCAGGAGCAATTTCGAGTTATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

484

Amino Acids

52.4

Weight (kDa)

8.88

Isoelectric Point (pI)

32.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 139 - 257 3.7e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 140 - 455 2.3e-18 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 141 - 460 1.5e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10840 AT3G10840 AT3G10840 AT3G10840
fragaria_vesca FvH4_7g26310
malus_domestica MD07G1238400.v1.1
prunus_persica Prupe.2G267200_v2.0.a1 Prupe.2G267200_v2.0.a1
pyrus_communis pycom07g21440
rosa_chinensis RchiOBHm_Chr1g0372761
rosa_laevigata RLG00000026874
rosa_multiflora Rmu_co8126034.1_g000001 Rmu_sc0009717.1_g000006
rosa_roxburghii Rroxscaffold_4G00284700
rosa_rugosa Rorug01G0374200
rosa_samantha Rh1AG382900 Rh1BG346500 Rh1CG359700 Rh1DG377500
rosa_wichuraiana Rw1G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1268
AccB1I GGYRCC 1 cut(s) 173
AccBSI CCGCTC 1 cut(s) 740
AciI CCGC 4 cut(s) 14, 165, 738, 1407
AclWI GGATC 4 cut(s) 272, 285, 328, 808
AcoI YGGCCR 2 cut(s) 480, 735
AcsI RAATTY 5 cut(s) 844, 896, 974, 1037, 1394
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 3 cut(s) 182, 369, 1157
AfiI CCNNNNNNNGG 3 cut(s) 343, 527, 1311
AflIII ACRYGT 1 cut(s) 730
AgsI TTSAA 5 cut(s) 130, 719, 1299, 1325, 1383
AjnI CCWGG 1 cut(s) 808
Alw26I GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
AlwI GGATC 4 cut(s) 272, 285, 328, 808
AoxI GGCC 6 cut(s) 100, 480, 486, 518, 663, 735
ApeKI GCWGC 6 cut(s) 146, 149, 152, 653, 1049, 1163
ApoI RAATTY 5 cut(s) 844, 896, 974, 1037, 1394
AspLEI GCGC 1 cut(s) 996
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 1311
AsuHPI GGTGA 2 cut(s) 946, 1265
BaeGI GKGCMC 2 cut(s) 178, 1347
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 173
BbsI GAAGAC 2 cut(s) 126, 1101
BbvI GCAGC 6 cut(s) 133, 136, 139, 665, 1036, 1175
BccI CCATC 4 cut(s) 92, 345, 830, 937
BceAI ACGGC 2 cut(s) 460, 1176
BciT130I CCWGG 1 cut(s) 810
BclI TGATCA 1 cut(s) 256
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 1424
BisI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
BlsI GCNGC 8 cut(s) 148, 151, 154, 167, 655, 739, 1051, 1165
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 2 cut(s) 810, 1311
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 5 cut(s) 169, 175, 279, 442, 496
BmrFI CCNGG 2 cut(s) 810, 1311
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpiI GAAGAC 2 cut(s) 126, 1101
BplI GAGNNNNNCTC 2 cut(s) 371, 403
BpmI CTGGAG 1 cut(s) 213
BpuEI CTTGAG 2 cut(s) 61, 373
BpuMI CCSGG 1 cut(s) 1311
BsaJI CCNNGG 4 cut(s) 444, 483, 606, 1310
BsaWI WCCGGW 3 cut(s) 139, 170, 185
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 527, 1311
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 3 cut(s) 178, 273, 874
Bse3DI GCAATG 1 cut(s) 1173
BseBI CCWGG 1 cut(s) 810
BseDI CCNNGG 4 cut(s) 444, 483, 606, 1310
BseGI GGATG 1 cut(s) 948
BseLI CCNNNNNNNGG 3 cut(s) 343, 527, 1311
BseMI GCAATG 1 cut(s) 1173
BseMII CTCAG 3 cut(s) 369, 648, 818
BseNI ACTGG 3 cut(s) 178, 273, 874
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 2 cut(s) 178, 1347
BseXI GCAGC 6 cut(s) 133, 136, 139, 665, 1036, 1175
BseYI CCCAGC 1 cut(s) 757
BshFI GGCC 6 cut(s) 102, 482, 488, 520, 665, 737
BshNI GGYRCC 1 cut(s) 173
BsiSI CCGG 5 cut(s) 140, 171, 186, 521, 1310
BslFI GGGAC 1 cut(s) 1256
BslI CCNNNNNNNGG 3 cut(s) 343, 527, 1311
BsmAI GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
BsmFI GGGAC 1 cut(s) 1256
BsmI GAATGC 3 cut(s) 679, 1066, 1288
BsnI GGCC 6 cut(s) 102, 482, 488, 520, 665, 737
Bsp1286I GDGCHC 2 cut(s) 178, 1347
Bsp143I GATC 5 cut(s) 256, 277, 333, 420, 800
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 4 cut(s) 14, 165, 738, 1407
BspANI GGCC 6 cut(s) 102, 482, 488, 520, 665, 737
BspCNI CTCAG 3 cut(s) 370, 649, 817
BspLI GGNNCC 5 cut(s) 169, 175, 279, 442, 496
BspMAI CTGCAG 1 cut(s) 1428
BspPI GGATC 4 cut(s) 272, 285, 328, 808
BspQI GCTCTTC 1 cut(s) 993
BspT107I GGYRCC 1 cut(s) 173
BsrBI CCGCTC 1 cut(s) 740
BsrDI GCAATG 1 cut(s) 1173
BsrFI RCCGGY 1 cut(s) 520
BsrI ACTGG 3 cut(s) 178, 273, 874
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 4 cut(s) 444, 483, 606, 1310
BssMI GATC 5 cut(s) 256, 277, 333, 420, 800
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 1 cut(s) 810
Bst4CI ACNGT 2 cut(s) 346, 406
Bst6I CTCTTC 3 cut(s) 993, 1283, 1353
BstC8I GCNNGC 2 cut(s) 522, 624
BstDEI CTNAG 5 cut(s) 378, 399, 657, 804, 882
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 1 cut(s) 948
BstHHI GCGC 1 cut(s) 996
BstKTI GATC 5 cut(s) 259, 280, 336, 423, 803
BstMAI GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
BstMBI GATC 5 cut(s) 256, 277, 333, 420, 800
BstMWI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 662, 767, 1342
BstNI CCWGG 1 cut(s) 810
BstSCI CCNGG 2 cut(s) 808, 1309
BstSFI CTRYAG 1 cut(s) 1424
BstSLI GKGCMC 2 cut(s) 178, 1347
BstV1I GCAGC 6 cut(s) 133, 136, 139, 665, 1036, 1175
BstV2I GAAGAC 2 cut(s) 126, 1101
BstX2I RGATCY 3 cut(s) 277, 333, 800
BstXI CCANNNNNNTGG 2 cut(s) 505, 673
BstYI RGATCY 3 cut(s) 277, 333, 800
BsuRI GGCC 6 cut(s) 102, 482, 488, 520, 665, 737
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 1 cut(s) 948
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 4 cut(s) 624, 708, 1086, 1108
Cac8I GCNNGC 2 cut(s) 522, 624
CfoI GCGC 1 cut(s) 996
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 101
Csp6I GTAC 3 cut(s) 181, 368, 1156
CviAII CATG 6 cut(s) 371, 431, 607, 1065, 1278, 1355
CviQI GTAC 3 cut(s) 181, 368, 1156
DdeI CTNAG 5 cut(s) 378, 399, 657, 804, 882
DpnI GATC 5 cut(s) 258, 279, 335, 422, 802
DpnII GATC 5 cut(s) 256, 277, 333, 420, 800
DrdI GACNNNNNNGTC 1 cut(s) 1268
DseDI GACNNNNNNGTC 1 cut(s) 1268
EaeI YGGCCR 2 cut(s) 480, 735
Eam1104I CTCTTC 3 cut(s) 993, 1283, 1353
EarI CTCTTC 3 cut(s) 993, 1283, 1353
Eco130I CCWWGG 1 cut(s) 606
Eco32I GATATC 1 cut(s) 1225
Eco57I CTGAAG 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 808
EcoRV GATATC 1 cut(s) 1225
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 1 cut(s) 1066
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 6 cut(s) 374, 434, 610, 1068, 1281, 1358
FalI AAGNNNNNCTT 4 cut(s) 70, 102, 962, 994
FaqI GGGAC 1 cut(s) 1256
FatI CATG 6 cut(s) 370, 430, 606, 1064, 1277, 1354
FauI CCCGC 1 cut(s) 7
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
FokI GGATG 1 cut(s) 955
Fsp4HI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 995
GluI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
GsaI CCCAGC 1 cut(s) 761
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 6 cut(s) 102, 482, 488, 520, 665, 737
HapII CCGG 5 cut(s) 140, 171, 186, 521, 1310
HhaI GCGC 1 cut(s) 996
Hin1II CATG 6 cut(s) 374, 434, 610, 1068, 1281, 1358
Hin6I GCGC 1 cut(s) 994
HinP1I GCGC 1 cut(s) 994
HincII GTYRAC 3 cut(s) 534, 793, 1369
HindII GTYRAC 3 cut(s) 534, 793, 1369
HindIII AAGCTT 2 cut(s) 79, 284
HinfI GANTC 9 cut(s) 66, 581, 595, 707, 862, 1073, 1190, 1215, 1418
HpaII CCGG 5 cut(s) 140, 171, 186, 521, 1310
HphI GGTGA 2 cut(s) 946, 1265
Hpy166II GTNNAC 4 cut(s) 534, 793, 1158, 1369
Hpy188I TCNGA 2 cut(s) 1189, 1242
Hpy188III TCNNGA 4 cut(s) 90, 192, 260, 570
Hpy8I GTNNAC 4 cut(s) 534, 793, 1158, 1369
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 3 cut(s) 229, 655, 1123
HpyCH4III ACNGT 2 cut(s) 346, 406
HpyCH4IV ACGT 2 cut(s) 120, 730
HpyCH4V TGCA 5 cut(s) 39, 211, 626, 1064, 1426
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 662, 767, 1342
HpyF3I CTNAG 5 cut(s) 378, 399, 657, 804, 882
HpySE526I ACGT 2 cut(s) 120, 730
Hsp92II CATG 6 cut(s) 374, 434, 610, 1068, 1281, 1358
HspAI GCGC 1 cut(s) 994
KroI GCCGGC 1 cut(s) 520
KroNI GCCGGC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 5 cut(s) 256, 277, 333, 420, 800
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 4 cut(s) 6, 173, 440, 1429
Lsp1109I GCAGC 6 cut(s) 133, 136, 139, 665, 1036, 1175
MaeI CTAG 1 cut(s) 540
MaeII ACGT 2 cut(s) 120, 730
MaeIII GTNAC 3 cut(s) 426, 1084, 1246
MalI GATC 5 cut(s) 258, 279, 335, 422, 802
MbiI CCGCTC 1 cut(s) 740
MboI GATC 5 cut(s) 256, 277, 333, 420, 800
MflI RGATCY 3 cut(s) 277, 333, 800
MhlI GDGCHC 2 cut(s) 178, 1347
MlyI GAGTC 4 cut(s) 60, 716, 1067, 1209
MnlI CCTC 6 cut(s) 188, 232, 268, 454, 478, 1116
Mph1103I ATGCAT 1 cut(s) 1066
MroNI GCCGGC 1 cut(s) 520
MseI TTAA 3 cut(s) 57, 362, 1389
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 3 cut(s) 165, 656, 686
MspI CCGG 5 cut(s) 140, 171, 186, 521, 1310
MspR9I CCNGG 2 cut(s) 810, 1311
Mva1269I GAATGC 3 cut(s) 679, 1066, 1288
MvaI CCWGG 1 cut(s) 810
MwoI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 662, 767, 1342
NaeI GCCGGC 1 cut(s) 522
NciI CCSGG 1 cut(s) 1311
NcoI CCATGG 1 cut(s) 606
NdeII GATC 5 cut(s) 256, 277, 333, 420, 800
NgoMIV GCCGGC 1 cut(s) 520
NlaIII CATG 6 cut(s) 374, 434, 610, 1068, 1281, 1358
NlaIV GGNNCC 5 cut(s) 169, 175, 279, 442, 496
NmeAIII GCCGAG 2 cut(s) 57, 508
NmuCI GTSAC 1 cut(s) 1084
NsiI ATGCAT 1 cut(s) 1066
PciSI GCTCTTC 1 cut(s) 993
PctI GAATGC 3 cut(s) 679, 1066, 1288
PdiI GCCGGC 1 cut(s) 522
PfeI GAWTC 5 cut(s) 581, 595, 862, 1190, 1418
PkrI GCNGC 8 cut(s) 148, 151, 154, 167, 655, 739, 1051, 1165
PleI GAGTC 4 cut(s) 60, 715, 1067, 1209
PpsI GAGTC 4 cut(s) 60, 715, 1067, 1209
Psp6I CCWGG 1 cut(s) 808
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 1 cut(s) 808
PspN4I GGNNCC 5 cut(s) 169, 175, 279, 442, 496
PspPI GGNCC 1 cut(s) 101
PstI CTGCAG 1 cut(s) 1428
PsuI RGATCY 3 cut(s) 277, 333, 800
PvuII CAGCTG 2 cut(s) 656, 686
RsaI GTAC 3 cut(s) 182, 369, 1157
RsaNI GTAC 3 cut(s) 181, 368, 1156
RseI CAYNNNNRTG 1 cut(s) 671
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 3 cut(s) 57, 362, 1389
SatI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
Sau3AI GATC 5 cut(s) 256, 277, 333, 420, 800
Sau96I GGNCC 1 cut(s) 101
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 4 cut(s) 60, 716, 1067, 1209
ScrFI CCNGG 2 cut(s) 810, 1311
SduI GDGCHC 2 cut(s) 178, 1347
SfcI CTRYAG 1 cut(s) 1424
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 2 cut(s) 76, 388
SmoI CTYRAG 2 cut(s) 76, 388
SsiI CCGC 4 cut(s) 14, 165, 738, 1407
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 2 cut(s) 808, 1309
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 2 cut(s) 346, 406
TaiI ACGT 2 cut(s) 123, 733
TaqI TCGA 4 cut(s) 50, 89, 1076, 1438
TaqII GACCGA 1 cut(s) 1278
TatI WGTACW 2 cut(s) 180, 1155
TauI GCSGC 2 cut(s) 168, 740
TfiI GAWTC 5 cut(s) 581, 595, 862, 1190, 1418
Tru1I TTAA 3 cut(s) 57, 362, 1389
Tru9I TTAA 3 cut(s) 57, 362, 1389
TscAI CASTG 4 cut(s) 624, 708, 1093, 1108
TseFI GTSAC 1 cut(s) 1084
TseI GCWGC 6 cut(s) 146, 149, 152, 653, 1049, 1163
Tsp45I GTSAC 1 cut(s) 1084
TspDTI ATGAA 7 cut(s) 279, 366, 485, 628, 1107, 1173, 1371
TspGWI ACGGA 2 cut(s) 588, 1208
TspRI CASTG 4 cut(s) 624, 708, 1093, 1108
XapI RAATTY 5 cut(s) 844, 896, 974, 1037, 1394
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.