FvH4_7g26310

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010989
Physical Location & Seq
Forward (+)
19913078 .. 19915881
2804 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26310.t1

Sequence Viewer

Length: 1431 bp
ATGAGGAGCTTGGCGGGGACATGGGTGTTGCCGAATTGCAGTAAAGTTTCGATCTTTAATGGGAATCTCAGTAGCTCAAGGTTCGGTTCGGATACCCAAAACTGCGACGTTTTCAAGTCTTCTGCTTTTGTTGCTGCTGCTGCTTCCGTCAGCGGCTCCGGTGCTCAATACTCCGGTTCAGAGGGGTTGCTTGGTGTGCAAACAAAGAAGAAGAGTAAGGGGATAGCTGGCATTGATCAGGATGAGCTGGTGGAGGATCCTAAGCTTTTGGCAGACCCGGATAGTTGTTTTTGTGAGTTTAGGGGAGTTGAGATACATCATAAGGTGTGTGAAGCACAATTGGAGGTGCATGATGAGAGTGAGAGTGTGTCAAGTGAGGTTAAGAGTGTTGGTCTGCCAATGATCTTGTTACATGGCTTTGGAGCCTCAGTTTTCTCTTGGAATCGGGTTATGAAGCCGTTGGCTGAGGCTGTCGGTTCTAAAGTTCTGGCATTTGATAGGCCGGCATTTGGGTTGACATCAAGGGGGAACTCTGTCAGGGAGTTATCGTCTGGAAATGGGGAATCGAAACCTGTGAATCCATATTCCATGGCGTTTTCGGTGCTTGCAACTTTGTATTTCATTGATTTTCTGGCAGCTGAGAAGGCCATTATAGTGGGGCATTCAGCTGGTTCTCTTGTAGCAGTTGAGTCATATTTTGAAGCACCGGAACGTGTGGCTGCTCTAATCCTTGTTGCCCCAGCTATTTTTGCCCCAATCATAACACAGAAGGTTGTCAAGGGATCCCAACCTGGAACAAATGACCAGACAGAAGGTGACTCAAATTCAGTTGATGATGGGAATCCTTTTGTCCAGTTTTCCAAGGTTTTGTCCAACATTGCCAGATATATTTCACAGGCAATCATGGGATTGGTGAAAGGGATGGCAGTCATGCTCAACTCTTTGTACAAGAGATTCTTGTCTGCTCTTCTACGCTCTACTTTTGCGATAATGCTGGTAAGAATGGCTATCAATAAATTCGGGATCACTGCTGTTAGGAATGCATGGTATGACTCAAAGAAAGTCACTGATGAAGACATCAGTGGTTATACAAAGCCATTAAGGACGAAGGGTTGGGATAAGGCACTTGTGGAGTACACAGCAGCAATGCTCACAGACACTTCATCCGAATCAAAACCACCGTTGGCAAAGAGACTCCGTGATATCAAATGTCCTGTTCTGATTGTTACGGGCGATAATGACCGAATTGTCCCGTCATGGAATGCTGAGAGACTTTCAAAAGCCATACCAGGGTCTTTCTTTGAAGTAATAAAGCATTGTGGGCACTTGCCACATGAAGAGAAAGTTGACGAGTTTCTTTCAGTAGTTAAGAAATTTTTACAAAGCGCTTTTGATGATTCTCCGCAGGAGAGATTACAAGTTATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

477

Amino Acids

51.59

Weight (kDa)

6.73

Isoelectric Point (pI)

30.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 133 - 448 7.7e-22 alpha/beta hydrolase fold
Hydrolase_4 PF12146 134 - 251 1.1e-06 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 135 - 453 2.4e-22 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10840 AT3G10840 AT3G10840 AT3G10840
fragaria_vesca FvH4_7g26310
malus_domestica MD07G1238400.v1.1
prunus_persica Prupe.2G267200_v2.0.a1 Prupe.2G267200_v2.0.a1
pyrus_communis pycom07g21440
rosa_chinensis RchiOBHm_Chr1g0372761
rosa_laevigata RLG00000026874
rosa_multiflora Rmu_co8126034.1_g000001 Rmu_sc0009717.1_g000006
rosa_roxburghii Rroxscaffold_4G00284700
rosa_rugosa Rorug01G0374200
rosa_samantha Rh1AG382900 Rh1BG346500 Rh1CG359700 Rh1DG377500
rosa_wichuraiana Rw1G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1247
AciI CCGC 3 cut(s) 14, 153, 1403
AclWI GGATC 5 cut(s) 251, 264, 777, 790, 1031
AcsI RAATTY 3 cut(s) 823, 1016, 1373
AfaI GTAC 2 cut(s) 947, 1136
AfeI AGCGCT 1 cut(s) 1387
AfiI CCNNNNNNNGG 2 cut(s) 509, 1290
AflIII ACRYGT 1 cut(s) 712
AgsI TTSAA 4 cut(s) 115, 701, 1278, 1304
AjnI CCWGG 2 cut(s) 790, 1288
AluBI AGCT 8 cut(s) 9, 75, 227, 247, 265, 638, 668, 743
AluI AGCT 8 cut(s) 9, 75, 227, 247, 265, 638, 668, 743
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 2 cut(s) 1186, 1264
AlwI GGATC 5 cut(s) 251, 264, 777, 790, 1031
Aor51HI AGCGCT 1 cut(s) 1387
AoxI GGCC 2 cut(s) 500, 645
ApeKI GCWGC 6 cut(s) 134, 137, 140, 635, 719, 1142
ApoI RAATTY 3 cut(s) 823, 1016, 1373
AspLEI GCGC 1 cut(s) 1388
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 827, 925
BaeGI GKGCMC 1 cut(s) 1326
BamHI GGATCC 2 cut(s) 256, 782
BbsI GAAGAC 2 cut(s) 111, 1080
Bbv12I GWGCWC 1 cut(s) 166
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 6 cut(s) 121, 124, 127, 647, 706, 1154
BccI CCATC 2 cut(s) 830, 916
BceAI ACGGC 1 cut(s) 442
BciT130I CCWGG 2 cut(s) 792, 1290
BciVI GTATCC 1 cut(s) 85
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 2 cut(s) 1186, 1264
BfoI RGCGCY 1 cut(s) 1389
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 7 cut(s) 135, 138, 141, 154, 636, 720, 1143
BlsI GCNGC 7 cut(s) 136, 139, 142, 155, 637, 721, 1144
Bme1390I CCNGG 3 cut(s) 278, 792, 1290
BmiI GGNNCC 4 cut(s) 157, 258, 424, 784
BmrFI CCNGG 3 cut(s) 278, 792, 1290
BpiI GAAGAC 2 cut(s) 111, 1080
Bpu10I CCTNAGC 2 cut(s) 261, 465
BpuEI CTTGAG 1 cut(s) 61
BpuMI CCSGG 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 840
BsaJI CCNNGG 3 cut(s) 588, 861, 1289
BsaWI WCCGGW 3 cut(s) 158, 173, 706
BsaXI ACNNNNNCTCC 4 cut(s) 414, 444, 533, 563
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 1290
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 1 cut(s) 853
Bse3DI GCAATG 2 cut(s) 876, 1152
Bse8I GATNNNNATC 1 cut(s) 840
BseBI CCWGG 2 cut(s) 792, 1290
BseDI CCNNGG 3 cut(s) 588, 861, 1289
BseGI GGATG 3 cut(s) 247, 927, 1163
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 2 cut(s) 509, 1290
BseMI GCAATG 2 cut(s) 876, 1152
BseMII CTCAG 5 cut(s) 82, 441, 456, 630, 1257
BseNI ACTGG 1 cut(s) 853
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 1326
BseXI GCAGC 6 cut(s) 121, 124, 127, 647, 706, 1154
BseYI CCCAGC 1 cut(s) 739
BshFI GGCC 2 cut(s) 502, 647
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 5 cut(s) 159, 174, 278, 503, 707
BslFI GGGAC 2 cut(s) 31, 1235
BslI CCNNNNNNNGG 2 cut(s) 509, 1290
BsmAI GTCTC 2 cut(s) 1186, 1264
BsmFI GGGAC 2 cut(s) 31, 1235
BsmI GAATGC 3 cut(s) 661, 1045, 1267
BsnI GGCC 2 cut(s) 502, 647
Bsp1286I GDGCHC 2 cut(s) 166, 1326
Bsp1407I TGTACA 1 cut(s) 945
Bsp143I GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 3 cut(s) 14, 153, 1403
BspANI GGCC 2 cut(s) 502, 647
BspCNI CTCAG 5 cut(s) 81, 440, 457, 631, 1258
BspLI GGNNCC 4 cut(s) 157, 258, 424, 784
BspPI GGATC 5 cut(s) 251, 264, 777, 790, 1031
BspQI GCTCTTC 1 cut(s) 972
BsrDI GCAATG 2 cut(s) 876, 1152
BsrFI RCCGGY 1 cut(s) 502
BsrGI TGTACA 1 cut(s) 945
BsrI ACTGG 1 cut(s) 853
BssAI RCCGGY 1 cut(s) 502
BssECI CCNNGG 3 cut(s) 588, 861, 1289
BssMI GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
BssT1I CCWWGG 2 cut(s) 588, 861
Bst2UI CCWGG 2 cut(s) 792, 1290
Bst4CI ACNGT 1 cut(s) 1182
Bst6I CTCTTC 3 cut(s) 206, 972, 1332
BstAUI TGTACA 1 cut(s) 945
BstC8I GCNNGC 3 cut(s) 229, 504, 606
BstDEI CTNAG 6 cut(s) 68, 261, 427, 465, 639, 1266
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 3 cut(s) 247, 927, 1163
BstH2I RGCGCY 1 cut(s) 1389
BstHHI GCGC 1 cut(s) 1388
BstKTI GATC 6 cut(s) 54, 238, 259, 405, 785, 1026
BstMAI GTCTC 2 cut(s) 1186, 1264
BstMBI GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
BstMWI GCNNNNNNNGC 6 cut(s) 131, 140, 196, 644, 749, 1321
BstNI CCWGG 2 cut(s) 792, 1290
BstSCI CCNGG 3 cut(s) 276, 790, 1288
BstSLI GKGCMC 1 cut(s) 1326
BstV1I GCAGC 6 cut(s) 121, 124, 127, 647, 706, 1154
BstV2I GAAGAC 2 cut(s) 111, 1080
BstX2I RGATCY 2 cut(s) 256, 782
BstXI CCANNNNNNTGG 1 cut(s) 655
BstYI RGATCY 2 cut(s) 256, 782
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 2 cut(s) 502, 647
BtgI CCRYGG 1 cut(s) 588
BtsCI GGATG 3 cut(s) 247, 927, 1163
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 3 cut(s) 1026, 1065, 1087
Cac8I GCNNGC 3 cut(s) 229, 504, 606
CfoI GCGC 1 cut(s) 1388
Cfr10I RCCGGY 1 cut(s) 502
Csp6I GTAC 2 cut(s) 946, 1135
CviAII CATG 9 cut(s) 21, 350, 413, 589, 904, 931, 1044, 1257, 1334
CviQI GTAC 2 cut(s) 946, 1135
DdeI CTNAG 6 cut(s) 68, 261, 427, 465, 639, 1266
DpnI GATC 6 cut(s) 53, 237, 258, 404, 784, 1025
DpnII GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
DrdI GACNNNNNNGTC 1 cut(s) 1247
DseDI GACNNNNNNGTC 1 cut(s) 1247
Eam1104I CTCTTC 3 cut(s) 206, 972, 1332
EarI CTCTTC 3 cut(s) 206, 972, 1332
Eco130I CCWWGG 2 cut(s) 588, 861
Eco32I GATATC 1 cut(s) 1204
Eco47III AGCGCT 1 cut(s) 1387
EcoRII CCWGG 2 cut(s) 790, 1288
EcoRV GATATC 1 cut(s) 1204
EcoT14I CCWWGG 2 cut(s) 588, 861
EcoT22I ATGCAT 1 cut(s) 1045
ErhI CCWWGG 2 cut(s) 588, 861
FaeI CATG 9 cut(s) 24, 353, 416, 592, 907, 934, 1047, 1260, 1337
FalI AAGNNNNNCTT 2 cut(s) 941, 973
FaqI GGGAC 2 cut(s) 31, 1235
FatI CATG 9 cut(s) 20, 349, 412, 588, 903, 930, 1043, 1256, 1333
FauI CCCGC 1 cut(s) 7
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 7 cut(s) 135, 138, 141, 154, 636, 720, 1143
FokI GGATG 3 cut(s) 254, 934, 1150
Fsp4HI GCNGC 7 cut(s) 135, 138, 141, 154, 636, 720, 1143
GlaI GCGC 1 cut(s) 1387
GluI GCNGC 7 cut(s) 135, 138, 141, 154, 636, 720, 1143
GsaI CCCAGC 1 cut(s) 743
HaeII RGCGCY 1 cut(s) 1389
HaeIII GGCC 2 cut(s) 502, 647
HapII CCGG 5 cut(s) 159, 174, 278, 503, 707
HhaI GCGC 1 cut(s) 1388
Hin1II CATG 9 cut(s) 24, 353, 416, 592, 907, 934, 1047, 1260, 1337
Hin6I GCGC 1 cut(s) 1386
HinP1I GCGC 1 cut(s) 1386
HincII GTYRAC 2 cut(s) 516, 1348
HindII GTYRAC 2 cut(s) 516, 1348
HindIII AAGCTT 1 cut(s) 263
HpaII CCGG 5 cut(s) 159, 174, 278, 503, 707
HphI GGTGA 2 cut(s) 827, 925
Hpy166II GTNNAC 3 cut(s) 516, 1137, 1348
Hpy188I TCNGA 4 cut(s) 91, 181, 1168, 1221
Hpy188III TCNNGA 3 cut(s) 239, 552, 1021
Hpy8I GTNNAC 3 cut(s) 516, 1137, 1348
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 4 cut(s) 637, 763, 806, 1102
HpyCH4III ACNGT 1 cut(s) 1182
HpyCH4IV ACGT 2 cut(s) 108, 712
HpyCH4V TGCA 5 cut(s) 39, 199, 349, 608, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 140, 196, 644, 749, 1321
HpyF3I CTNAG 6 cut(s) 68, 261, 427, 465, 639, 1266
HpySE526I ACGT 2 cut(s) 108, 712
Hsp92II CATG 9 cut(s) 24, 353, 416, 592, 907, 934, 1047, 1260, 1337
HspAI GCGC 1 cut(s) 1386
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 3 cut(s) 6, 161, 422
Lsp1109I GCAGC 6 cut(s) 121, 124, 127, 647, 706, 1154
MaeII ACGT 2 cut(s) 108, 712
MaeIII GTNAC 4 cut(s) 408, 815, 1063, 1225
MalI GATC 6 cut(s) 53, 237, 258, 404, 784, 1025
MboI GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
MboII GAAGA 6 cut(s) 111, 220, 223, 959, 1085, 1349
MfeI CAATTG 1 cut(s) 338
MflI RGATCY 2 cut(s) 256, 782
MhlI GDGCHC 2 cut(s) 166, 1326
MluCI AATT 6 cut(s) 34, 338, 823, 1016, 1245, 1373
MlyI GAGTC 4 cut(s) 698, 812, 1046, 1188
MmeI TCCRAC 1 cut(s) 897
MnlI CCTC 6 cut(s) 175, 247, 337, 370, 436, 460
Mph1103I ATGCAT 1 cut(s) 1045
MroNI GCCGGC 1 cut(s) 502
MseI TTAA 4 cut(s) 57, 381, 1100, 1368
MslI CAYNNNNRTG 1 cut(s) 653
MspA1I CMGCKG 3 cut(s) 153, 638, 668
MspI CCGG 5 cut(s) 159, 174, 278, 503, 707
MspR9I CCNGG 3 cut(s) 278, 792, 1290
MunI CAATTG 1 cut(s) 338
Mva1269I GAATGC 3 cut(s) 661, 1045, 1267
MvaI CCWGG 2 cut(s) 792, 1290
MwoI GCNNNNNNNGC 6 cut(s) 131, 140, 196, 644, 749, 1321
NaeI GCCGGC 1 cut(s) 504
NciI CCSGG 1 cut(s) 278
NcoI CCATGG 1 cut(s) 588
NdeII GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
NgoMIV GCCGGC 1 cut(s) 502
NlaIII CATG 9 cut(s) 24, 353, 416, 592, 907, 934, 1047, 1260, 1337
NlaIV GGNNCC 4 cut(s) 157, 258, 424, 784
NmuCI GTSAC 2 cut(s) 815, 1063
NsiI ATGCAT 1 cut(s) 1045
PciSI GCTCTTC 1 cut(s) 972
PctI GAATGC 3 cut(s) 661, 1045, 1267
PdiI GCCGGC 1 cut(s) 504
PfeI GAWTC 8 cut(s) 64, 442, 563, 577, 841, 954, 1169, 1397
PkrI GCNGC 7 cut(s) 136, 139, 142, 155, 637, 721, 1144
PleI GAGTC 4 cut(s) 697, 812, 1046, 1188
PpsI GAGTC 4 cut(s) 697, 812, 1046, 1188
Psp6I CCWGG 2 cut(s) 790, 1288
PspFI CCCAGC 1 cut(s) 739
PspGI CCWGG 2 cut(s) 790, 1288
PspN4I GGNNCC 4 cut(s) 157, 258, 424, 784
PsuI RGATCY 2 cut(s) 256, 782
PvuII CAGCTG 2 cut(s) 638, 668
RsaI GTAC 2 cut(s) 947, 1136
RsaNI GTAC 2 cut(s) 946, 1135
RseI CAYNNNNRTG 1 cut(s) 653
SapI GCTCTTC 1 cut(s) 972
SaqAI TTAA 4 cut(s) 57, 381, 1100, 1368
SatI GCNGC 7 cut(s) 135, 138, 141, 154, 636, 720, 1143
Sau3AI GATC 6 cut(s) 51, 235, 256, 402, 782, 1023
SchI GAGTC 4 cut(s) 698, 812, 1046, 1188
ScrFI CCNGG 3 cut(s) 278, 792, 1290
SduI GDGCHC 2 cut(s) 166, 1326
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 6 cut(s) 34, 338, 823, 1016, 1245, 1373
SsiI CCGC 3 cut(s) 14, 153, 1403
StyD4I CCNGG 3 cut(s) 276, 790, 1288
StyI CCWWGG 2 cut(s) 588, 861
TaaI ACNGT 1 cut(s) 1182
TaiI ACGT 2 cut(s) 111, 715
TaqI TCGA 2 cut(s) 50, 566
TaqII GACCGA 1 cut(s) 1257
TasI AATT 6 cut(s) 34, 338, 823, 1016, 1245, 1373
TatI WGTACW 2 cut(s) 945, 1134
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 8 cut(s) 64, 442, 563, 577, 841, 954, 1169, 1397
Tru1I TTAA 4 cut(s) 57, 381, 1100, 1368
Tru9I TTAA 4 cut(s) 57, 381, 1100, 1368
TscAI CASTG 3 cut(s) 1033, 1072, 1087
TseFI GTSAC 2 cut(s) 815, 1063
TseI GCWGC 6 cut(s) 134, 137, 140, 635, 719, 1142
Tsp45I GTSAC 2 cut(s) 815, 1063
TspDTI ATGAA 5 cut(s) 467, 610, 1086, 1152, 1350
TspGWI ACGGA 2 cut(s) 136, 1187
TspRI CASTG 3 cut(s) 1033, 1072, 1087
XapI RAATTY 3 cut(s) 823, 1016, 1373
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.