Rh1DG377500

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010989
Physical Location & Seq
Forward (+)
59967569 .. 59970216
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG377500.1

Sequence Viewer

Length: 1449 bp
ATGAGGAGCTTGGCGGGGATATGGGTGTTACCGAGTTGCAGTAAAGTTTCGAGCTTTAATGGCAGACTCATTAGCTCCAGCTTTGCTTCGAGATTTGGTGGCCCAGATACTCCAAACTACGTCGTTTTGAAGTCTTCTCCGGTGGCTGCTGCTGCTTCAGTCAGCGGCTCCGGTGCCCAGTACTCCGGTTCTGGGGGATTGCTTGGTGTGCAAACTAAGGAGGAGAAGAAGAAGGAGGGAGTAGCTGGGATTGATCAAGATGAACTGGTGGAGGATCCAAAGCTTTTAGCTGACCCAGATAGTTGTTTTTGTGAGTTTAGTGGAGTGGAGATACATCATAAGGTATATGAAGCACAATTGAAGGTGCATGAGACTGAGAGTGTCTCAAGTGAGACTAAGACTGTTGGTCTTGCTATGATCTTGTTACATGGGTTTGGAGCCTCGGTTTTCTCTTGGAAACGGGTTATGAAGCCGTTGGCCGAGGCCATTGGTTCCAAAGTTCTGGCGTATGATAGGCCGGCGTTTGGCTTGACATCTAGGGGAAACTCTGTCAGGCATTTATCTTCTGCAAATGGGGAATCGAAACCTGTGAATCCGTATTCCATGGCATTTTCAGTGCTTGCAACCTTGTATTTCATTGATTTTCTGGCAGCTGAGAAGGCCATTATAGTGGGGCATTCAGCTGGTTCTCTTGTAGCAGTTGAGTCATATTTTGAAGCACCGGAACGTGTGGCCGCTCTAATCCTTGTTGCCCCAGCCATTTTTGCCCCAATTACAACCCAGAAGGTTGACAAGGGATCTCAACCTGGAACAAATAACCAGACAGAAGAAGATGGCTCAAATTCAGTTGATAATGGGAATCCATTTGTCCAGTTCTCTAAGGTTCTGTCCAAATTCGCCAGATTTATTTCACAGGCAATTATGGGATTGGTGAAAGGAATGGCGGTTATGCTCAACTCTTTGTATAAGAAATTCTTGTCAGTTCTTCTGCGCTCTGCTTTTGCGATAATGCTGGTAAGAATGGCAATCAATAAATTCGGTATCGCTGCTGTTAGGAATGCATGGTATGACTCAAAGAAAGTCACTGATGAAGACATCAGTGGTTATACAAAGCCATTGAGGACGAAGGGTTGGGATAAGGCACTTGTGGAGTACACGGCAGCAATGCTGACAGACACTTCATCTGAATCAAAGCCACCATTGGCAAAGAGACTCCGTGATATCAAATGTCCTGTTCTGATTGTTACGGGTGATACTGACCGAATTGTCCCATCATGGAATGCGAAGAGACTTTCAAAAGCCATACCAGGGTCTTGCTTTGAAGTAATAAAGCGTTGTGGGCACTTGCCACATGAAGAGAAAGATGACAAGTTTGTTTCAATAGTTAAGAAATTTCTACAAACCACTTTTGATGATTCTCTGCAGGAGCAATTACGAGTTATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

482

Amino Acids

52.24

Weight (kDa)

8.96

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 138 - 256 2.2e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 139 - 454 1.1e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 140 - 459 2e-21 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10840 AT3G10840 AT3G10840 AT3G10840
fragaria_vesca FvH4_7g26310
malus_domestica MD07G1238400.v1.1
prunus_persica Prupe.2G267200_v2.0.a1 Prupe.2G267200_v2.0.a1
pyrus_communis pycom07g21440
rosa_chinensis RchiOBHm_Chr1g0372761
rosa_laevigata RLG00000026874
rosa_multiflora Rmu_co8126034.1_g000001 Rmu_sc0009717.1_g000006
rosa_roxburghii Rroxscaffold_4G00284700
rosa_rugosa Rorug01G0374200
rosa_samantha Rh1AG382900 Rh1BG346500 Rh1CG359700 Rh1DG377500
rosa_wichuraiana Rw1G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1265
AccB1I GGYRCC 1 cut(s) 173
AccBSI CCGCTC 1 cut(s) 737
AciI CCGC 4 cut(s) 14, 165, 735, 944
AclWI GGATC 3 cut(s) 269, 282, 805
AcoI YGGCCR 2 cut(s) 477, 732
AcsI RAATTY 5 cut(s) 841, 893, 971, 1034, 1391
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 2 cut(s) 182, 1154
AfiI CCNNNNNNNGG 3 cut(s) 192, 524, 1308
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 6 cut(s) 130, 361, 716, 1296, 1322, 1380
AhdI GACNNNNNGTC 1 cut(s) 405
AjnI CCWGG 2 cut(s) 805, 1306
AluBI AGCT 9 cut(s) 9, 54, 75, 81, 245, 283, 290, 653, 683
AluI AGCT 9 cut(s) 9, 54, 75, 81, 245, 283, 290, 653, 683
Alw26I GTCTC 5 cut(s) 365, 386, 388, 1204, 1282
AlwI GGATC 3 cut(s) 269, 282, 805
AoxI GGCC 6 cut(s) 100, 477, 483, 515, 660, 732
ApeKI GCWGC 6 cut(s) 146, 149, 152, 650, 1046, 1160
ApoI RAATTY 5 cut(s) 841, 893, 971, 1034, 1391
AspLEI GCGC 1 cut(s) 993
AspS9I GGNCC 1 cut(s) 101
AsuHPI GGTGA 2 cut(s) 943, 1262
BaeGI GKGCMC 2 cut(s) 178, 1344
BamHI GGATCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 173
BbsI GAAGAC 2 cut(s) 126, 1098
BbvI GCAGC 6 cut(s) 133, 136, 139, 662, 1033, 1172
BccI CCATC 2 cut(s) 827, 1279
BceAI ACGGC 2 cut(s) 457, 1173
BciT130I CCWGG 2 cut(s) 807, 1308
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 5 cut(s) 365, 386, 388, 1204, 1282
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 1 cut(s) 1421
BisI GCNGC 8 cut(s) 147, 150, 153, 166, 651, 735, 1047, 1161
BlsI GCNGC 8 cut(s) 148, 151, 154, 167, 652, 736, 1048, 1162
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 2 cut(s) 807, 1308
BmeRI GACNNNNNGTC 1 cut(s) 405
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 5 cut(s) 169, 175, 276, 439, 493
BmrFI CCNGG 2 cut(s) 807, 1308
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpiI GAAGAC 2 cut(s) 126, 1098
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpmI CTGGAG 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 370
BsaJI CCNNGG 4 cut(s) 441, 480, 603, 1307
BsaWI WCCGGW 4 cut(s) 139, 170, 185, 721
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 524, 1308
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 3 cut(s) 178, 270, 871
Bse3DI GCAATG 1 cut(s) 1170
BseBI CCWGG 2 cut(s) 807, 1308
BseDI CCNNGG 4 cut(s) 441, 480, 603, 1307
BseLI CCNNNNNNNGG 3 cut(s) 192, 524, 1308
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 2 cut(s) 366, 645
BseNI ACTGG 3 cut(s) 178, 270, 871
BseRI GAGGAG 2 cut(s) 19, 236
BseSI GKGCMC 2 cut(s) 178, 1344
BseXI GCAGC 6 cut(s) 133, 136, 139, 662, 1033, 1172
BseYI CCCAGC 2 cut(s) 245, 754
BshFI GGCC 6 cut(s) 102, 479, 485, 517, 662, 734
BshNI GGYRCC 1 cut(s) 173
BsiSI CCGG 5 cut(s) 140, 171, 186, 518, 722
BslFI GGGAC 1 cut(s) 1253
BslI CCNNNNNNNGG 3 cut(s) 192, 524, 1308
BsmAI GTCTC 5 cut(s) 365, 386, 388, 1204, 1282
BsmFI GGGAC 1 cut(s) 1253
BsmI GAATGC 3 cut(s) 676, 1063, 1285
BsnI GGCC 6 cut(s) 102, 479, 485, 517, 662, 734
Bsp1286I GDGCHC 2 cut(s) 178, 1344
Bsp143I GATC 4 cut(s) 253, 274, 417, 797
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 4 cut(s) 14, 165, 735, 944
BspANI GGCC 6 cut(s) 102, 479, 485, 517, 662, 734
BspCNI CTCAG 2 cut(s) 367, 646
BspLI GGNNCC 5 cut(s) 169, 175, 276, 439, 493
BspMAI CTGCAG 1 cut(s) 1425
BspPI GGATC 3 cut(s) 269, 282, 805
BspT107I GGYRCC 1 cut(s) 173
BsrBI CCGCTC 1 cut(s) 737
BsrDI GCAATG 1 cut(s) 1170
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 3 cut(s) 178, 270, 871
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 4 cut(s) 441, 480, 603, 1307
BssMI GATC 4 cut(s) 253, 274, 417, 797
BssT1I CCWWGG 1 cut(s) 603
Bst2UI CCWGG 2 cut(s) 807, 1308
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 2 cut(s) 1280, 1350
BstC8I GCNNGC 2 cut(s) 519, 621
BstDEI CTNAG 5 cut(s) 216, 375, 396, 654, 879
BstDSI CCRYGG 1 cut(s) 603
BstHHI GCGC 1 cut(s) 993
BstKTI GATC 4 cut(s) 256, 277, 420, 800
BstMAI GTCTC 5 cut(s) 365, 386, 388, 1204, 1282
BstMBI GATC 4 cut(s) 253, 274, 417, 797
BstMWI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 659, 764, 1339
BstNI CCWGG 2 cut(s) 807, 1308
BstSCI CCNGG 2 cut(s) 805, 1306
BstSFI CTRYAG 1 cut(s) 1421
BstSLI GKGCMC 2 cut(s) 178, 1344
BstV1I GCAGC 6 cut(s) 133, 136, 139, 662, 1033, 1172
BstV2I GAAGAC 2 cut(s) 126, 1098
BstX2I RGATCY 2 cut(s) 274, 797
BstXI CCANNNNNNTGG 2 cut(s) 502, 670
BstYI RGATCY 2 cut(s) 274, 797
BsuRI GGCC 6 cut(s) 102, 479, 485, 517, 662, 734
BtgI CCRYGG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 621, 1083, 1105
Cac8I GCNNGC 2 cut(s) 519, 621
CfoI GCGC 1 cut(s) 993
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 181, 1153
CviAII CATG 6 cut(s) 368, 428, 604, 1062, 1275, 1352
CviQI GTAC 2 cut(s) 181, 1153
DdeI CTNAG 5 cut(s) 216, 375, 396, 654, 879
DpnI GATC 4 cut(s) 255, 276, 419, 799
DpnII GATC 4 cut(s) 253, 274, 417, 797
DrdI GACNNNNNNGTC 1 cut(s) 1265
DriI GACNNNNNGTC 1 cut(s) 405
DseDI GACNNNNNNGTC 1 cut(s) 1265
EaeI YGGCCR 2 cut(s) 477, 732
Eam1104I CTCTTC 2 cut(s) 1280, 1350
Eam1105I GACNNNNNGTC 1 cut(s) 405
EarI CTCTTC 2 cut(s) 1280, 1350
Eco130I CCWWGG 1 cut(s) 603
Eco32I GATATC 1 cut(s) 1222
Eco57I CTGAAG 1 cut(s) 141
EcoRII CCWGG 2 cut(s) 805, 1306
EcoRV GATATC 1 cut(s) 1222
EcoT14I CCWWGG 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 1063
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 6 cut(s) 371, 431, 607, 1065, 1278, 1355
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FaqI GGGAC 1 cut(s) 1253
FatI CATG 6 cut(s) 367, 427, 603, 1061, 1274, 1351
FauI CCCGC 1 cut(s) 7
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 8 cut(s) 147, 150, 153, 166, 651, 735, 1047, 1161
Fsp4HI GCNGC 8 cut(s) 147, 150, 153, 166, 651, 735, 1047, 1161
FspBI CTAG 1 cut(s) 537
GlaI GCGC 1 cut(s) 992
GluI GCNGC 8 cut(s) 147, 150, 153, 166, 651, 735, 1047, 1161
GsaI CCCAGC 2 cut(s) 249, 758
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 6 cut(s) 102, 479, 485, 517, 662, 734
HapII CCGG 5 cut(s) 140, 171, 186, 518, 722
HhaI GCGC 1 cut(s) 993
Hin1II CATG 6 cut(s) 371, 431, 607, 1065, 1278, 1355
Hin6I GCGC 1 cut(s) 991
HinP1I GCGC 1 cut(s) 991
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 9 cut(s) 66, 578, 592, 704, 859, 1070, 1187, 1212, 1415
HpaII CCGG 5 cut(s) 140, 171, 186, 518, 722
HphI GGTGA 2 cut(s) 943, 1262
Hpy166II GTNNAC 2 cut(s) 790, 1155
Hpy188I TCNGA 2 cut(s) 1186, 1239
Hpy188III TCNNGA 2 cut(s) 90, 257
Hpy8I GTNNAC 2 cut(s) 790, 1155
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 5 cut(s) 226, 355, 652, 778, 1120
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 2 cut(s) 120, 727
HpyCH4V TGCA 7 cut(s) 39, 211, 367, 569, 623, 1061, 1423
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 659, 764, 1339
HpyF3I CTNAG 5 cut(s) 216, 375, 396, 654, 879
HpySE526I ACGT 2 cut(s) 120, 727
Hsp92II CATG 6 cut(s) 371, 431, 607, 1065, 1278, 1355
HspAI GCGC 1 cut(s) 991
KroI GCCGGC 1 cut(s) 517
KroNI GCCGGC 1 cut(s) 519
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 4 cut(s) 253, 274, 417, 797
LmnI GCTCC 5 cut(s) 6, 80, 173, 437, 1426
Lsp1109I GCAGC 6 cut(s) 133, 136, 139, 662, 1033, 1172
MaeI CTAG 1 cut(s) 537
MaeII ACGT 2 cut(s) 120, 727
MaeIII GTNAC 4 cut(s) 27, 423, 1081, 1243
MalI GATC 4 cut(s) 255, 276, 419, 799
MbiI CCGCTC 1 cut(s) 737
MboI GATC 4 cut(s) 253, 274, 417, 797
MfeI CAATTG 1 cut(s) 356
MflI RGATCY 2 cut(s) 274, 797
MhlI GDGCHC 2 cut(s) 178, 1344
MlyI GAGTC 4 cut(s) 60, 713, 1064, 1206
MnlI CCTC 6 cut(s) 214, 229, 265, 451, 475, 1113
Mph1103I ATGCAT 1 cut(s) 1063
MroNI GCCGGC 1 cut(s) 517
MseI TTAA 2 cut(s) 57, 1386
MslI CAYNNNNRTG 1 cut(s) 668
MspA1I CMGCKG 3 cut(s) 165, 653, 683
MspI CCGG 5 cut(s) 140, 171, 186, 518, 722
MspR9I CCNGG 2 cut(s) 807, 1308
MunI CAATTG 1 cut(s) 356
Mva1269I GAATGC 3 cut(s) 676, 1063, 1285
MvaI CCWGG 2 cut(s) 807, 1308
MwoI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 659, 764, 1339
NaeI GCCGGC 1 cut(s) 519
NcoI CCATGG 1 cut(s) 603
NdeII GATC 4 cut(s) 253, 274, 417, 797
NgoMIV GCCGGC 1 cut(s) 517
NlaIII CATG 6 cut(s) 371, 431, 607, 1065, 1278, 1355
NlaIV GGNNCC 5 cut(s) 169, 175, 276, 439, 493
NmeAIII GCCGAG 1 cut(s) 505
NmuCI GTSAC 1 cut(s) 1081
NsiI ATGCAT 1 cut(s) 1063
PctI GAATGC 3 cut(s) 676, 1063, 1285
PdiI GCCGGC 1 cut(s) 519
PfeI GAWTC 5 cut(s) 578, 592, 859, 1187, 1415
PkrI GCNGC 8 cut(s) 148, 151, 154, 167, 652, 736, 1048, 1162
PleI GAGTC 4 cut(s) 60, 712, 1064, 1206
PpsI GAGTC 4 cut(s) 60, 712, 1064, 1206
Psp6I CCWGG 2 cut(s) 805, 1306
PspFI CCCAGC 2 cut(s) 245, 754
PspGI CCWGG 2 cut(s) 805, 1306
PspN4I GGNNCC 5 cut(s) 169, 175, 276, 439, 493
PspPI GGNCC 1 cut(s) 101
PstI CTGCAG 1 cut(s) 1425
PsuI RGATCY 2 cut(s) 274, 797
PvuII CAGCTG 2 cut(s) 653, 683
RsaI GTAC 2 cut(s) 182, 1154
RsaNI GTAC 2 cut(s) 181, 1153
RseI CAYNNNNRTG 1 cut(s) 668
SaqAI TTAA 2 cut(s) 57, 1386
SatI GCNGC 8 cut(s) 147, 150, 153, 166, 651, 735, 1047, 1161
Sau3AI GATC 4 cut(s) 253, 274, 417, 797
Sau96I GGNCC 1 cut(s) 101
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 4 cut(s) 60, 713, 1064, 1206
ScrFI CCNGG 2 cut(s) 807, 1308
SduI GDGCHC 2 cut(s) 178, 1344
SfcI CTRYAG 1 cut(s) 1421
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
SsiI CCGC 4 cut(s) 14, 165, 735, 944
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 2 cut(s) 805, 1306
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 2 cut(s) 123, 730
TaqI TCGA 3 cut(s) 50, 89, 581
TaqII GACCGA 1 cut(s) 1275
TatI WGTACW 2 cut(s) 180, 1152
TauI GCSGC 2 cut(s) 168, 737
TfiI GAWTC 5 cut(s) 578, 592, 859, 1187, 1415
Tru1I TTAA 2 cut(s) 57, 1386
Tru9I TTAA 2 cut(s) 57, 1386
TscAI CASTG 3 cut(s) 621, 1090, 1105
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 6 cut(s) 146, 149, 152, 650, 1046, 1160
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 7 cut(s) 276, 363, 482, 625, 1104, 1170, 1368
TspGWI ACGGA 2 cut(s) 585, 1205
TspRI CASTG 3 cut(s) 621, 1090, 1105
XapI RAATTY 5 cut(s) 841, 893, 971, 1034, 1391
XspI CTAG 1 cut(s) 537
ZrmI AGTACT 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.