Rw1G033700

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010989
Physical Location & Seq
Forward (+)
61687896 .. 61690543
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033700.1

Sequence Viewer

Length: 1452 bp
ATGAGGAGCTTGGCGGGGATATGGGTGTTGCCGAGTTGCAGTAAAGTTTCGAGCTTTAATGGCAGACTCATTAGCTCAAGCTTTGCTTCGAGATTTGGTGGCCCAGATACTCCAAACTACGTCGTTTTGAAGTCTTCTCCGGTGGCTGCTGCTGCTTCAGTCGGCGGCTCCGGTGCCCAGTACTCCGGTTCTGGGGGATTGCTTGGTGTGCCAACTAAGGAGGAGAGGAAGAAGAAGGAGGGAATAGCTGGGATTGATCAAGATGAACTGGTGGAGGATCCAAAGCTTTTAGCTGACCCAGATAGTTGTTTCTGTGAGTTTAGTGGAGTGGAGATACATCATAAGGTATATGAAGCACAATTGAAGGTGCATGAGACTGAGAGTGTCTCAAGTGAGACCAAGACTGTTGGTCTTGCTATGATCTTGTTACATGGGTTTGGAGCCTCGGTTTTCTCTTGGAAACGGGTTATGAAGCCGTTGGCCGAGGCCATTGGTTCCAAAGTTCTGGCGTTTGATAGGCCGGCGTTTGGGTTGACATCTAGGGGAAACTCTGTCAGGCATTTATCTTCTGGAAATGGGGAATCCAAACCTGTGAATCCGTATTCCTTGGCATTTTCAGTGCTTGCAACCTTGTATTTCATTGATTTTCTGGCAGCTGAGAAGGCCATTATAGTGGGGCATTCAGCTGGTTCTCTTGTAGCAGTTGAGTCATATTTTGAAGCACCGGAACGTGTGGCTGCTCTAATCCTTGTTGCCCCAGCCATTTTTGCCCCAATTACAACCCAGAAGGTTGACAAGGGATCTCAACCTGGAACAAATAACCAGACAGAAGAAGATGGCTCAAATTCAGTTGATAATGGGAATCCATTTGTCCAGTTCTCTAAGGTTCTGTCCAAATTTGCCAGACTTATTTCACAGGCAATTATGGGATTGGTGAAAGGGATGGCAGTTATGCTCAACTCTTTGTATAAGAAATTCTTGTCGGTTCTTCTGCGCTCTGCTTTTGCGATAATGCTGGTAAGAATGGCAATCAATAAATTCGGTATCGCTGCTGTTAGGAATGCATGGTATGACTCGAAGAAAGTCACTGATGAAGACATCAGTGGTTATACAAAGCCATTGAGGACGAAGGGTTGGGATAAGGCACTTGTGGAGTACACGGCAGCAATGCTCACAGACACTTCATCTGAATCAAAGCCACCATTGGCAAAGAGACTCCGTGATATCAAATGTCCCGTTCTGATTGTTACGGGTGATACTGACCGAATTGTCCCATCATGGAATGCGAAGAGACTTTCAAAAGCCATACCAGGGTCTTGCTTTGAAGTAATAAAGCGTTGTGGGCACTTGCCACATGAAGAGAAAGTTGATGAGTTTGTTTCAATAGTTAAGAAATTTCTACAAACCGCTTATGATGATTCTCCGCAGGAGCAATTAGGAGTTATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

483

Amino Acids

52.12

Weight (kDa)

8.88

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 139 - 257 1e-06 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 140 - 455 1.2e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 141 - 460 1.6e-21 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10840 AT3G10840 AT3G10840 AT3G10840
fragaria_vesca FvH4_7g26310
malus_domestica MD07G1238400.v1.1
prunus_persica Prupe.2G267200_v2.0.a1 Prupe.2G267200_v2.0.a1
pyrus_communis pycom07g21440
rosa_chinensis RchiOBHm_Chr1g0372761
rosa_laevigata RLG00000026874
rosa_multiflora Rmu_co8126034.1_g000001 Rmu_sc0009717.1_g000006
rosa_roxburghii Rroxscaffold_4G00284700
rosa_rugosa Rorug01G0374200
rosa_samantha Rh1AG382900 Rh1BG346500 Rh1CG359700 Rh1DG377500
rosa_wichuraiana Rw1G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1268
AccB1I GGYRCC 1 cut(s) 173
AciI CCGC 4 cut(s) 14, 165, 1407, 1424
AclWI GGATC 3 cut(s) 272, 285, 808
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 5 cut(s) 844, 896, 974, 1037, 1394
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 2 cut(s) 182, 1157
AfiI CCNNNNNNNGG 3 cut(s) 192, 527, 1311
AflIII ACRYGT 1 cut(s) 730
AgsI TTSAA 6 cut(s) 130, 364, 719, 1299, 1325, 1383
AhdI GACNNNNNGTC 1 cut(s) 408
AjnI CCWGG 2 cut(s) 808, 1309
AluBI AGCT 9 cut(s) 9, 54, 75, 81, 248, 286, 293, 656, 686
AluI AGCT 9 cut(s) 9, 54, 75, 81, 248, 286, 293, 656, 686
Alw26I GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
AlwI GGATC 3 cut(s) 272, 285, 808
AoxI GGCC 5 cut(s) 100, 480, 486, 518, 663
ApeKI GCWGC 7 cut(s) 146, 149, 152, 653, 737, 1049, 1163
ApoI RAATTY 5 cut(s) 844, 896, 974, 1037, 1394
AspLEI GCGC 1 cut(s) 996
AspS9I GGNCC 1 cut(s) 101
AsuHPI GGTGA 2 cut(s) 946, 1265
BaeGI GKGCMC 2 cut(s) 178, 1347
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 173
BbsI GAAGAC 2 cut(s) 126, 1101
BbvI GCAGC 7 cut(s) 133, 136, 139, 665, 724, 1036, 1175
BccI CCATC 3 cut(s) 830, 937, 1282
BceAI ACGGC 2 cut(s) 460, 1176
BciT130I CCWGG 2 cut(s) 810, 1311
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
BfaI CTAG 1 cut(s) 540
BisI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
BlsI GCNGC 8 cut(s) 148, 151, 154, 167, 655, 739, 1051, 1165
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 2 cut(s) 810, 1311
BmeRI GACNNNNNGTC 1 cut(s) 408
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 5 cut(s) 169, 175, 279, 442, 496
BmrFI CCNGG 2 cut(s) 810, 1311
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpiI GAAGAC 2 cut(s) 126, 1101
BplI GAGNNNNNCTC 2 cut(s) 371, 403
BpuEI CTTGAG 2 cut(s) 61, 373
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 4 cut(s) 444, 483, 606, 1310
BsaWI WCCGGW 4 cut(s) 139, 170, 185, 724
BsaXI ACNNNNNCTCC 3 cut(s) 432, 462, 1431
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 527, 1311
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 3 cut(s) 178, 273, 874
Bse3DI GCAATG 1 cut(s) 1173
BseBI CCWGG 2 cut(s) 810, 1311
BseDI CCNNGG 4 cut(s) 444, 483, 606, 1310
BseGI GGATG 1 cut(s) 948
BseLI CCNNNNNNNGG 3 cut(s) 192, 527, 1311
BseMI GCAATG 1 cut(s) 1173
BseMII CTCAG 2 cut(s) 369, 648
BseNI ACTGG 3 cut(s) 178, 273, 874
BseRI GAGGAG 2 cut(s) 19, 236
BseSI GKGCMC 2 cut(s) 178, 1347
BseXI GCAGC 7 cut(s) 133, 136, 139, 665, 724, 1036, 1175
BseYI CCCAGC 2 cut(s) 248, 757
BshFI GGCC 5 cut(s) 102, 482, 488, 520, 665
BshNI GGYRCC 1 cut(s) 173
BsiSI CCGG 5 cut(s) 140, 171, 186, 521, 725
BslFI GGGAC 2 cut(s) 1218, 1256
BslI CCNNNNNNNGG 3 cut(s) 192, 527, 1311
BsmAI GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
BsmFI GGGAC 2 cut(s) 1218, 1256
BsmI GAATGC 3 cut(s) 679, 1066, 1288
BsnI GGCC 5 cut(s) 102, 482, 488, 520, 665
Bso31I GGTCTC 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 178, 1347
Bsp143I GATC 4 cut(s) 256, 277, 420, 800
BspACI CCGC 4 cut(s) 14, 165, 1407, 1424
BspANI GGCC 5 cut(s) 102, 482, 488, 520, 665
BspCNI CTCAG 2 cut(s) 370, 649
BspLI GGNNCC 5 cut(s) 169, 175, 279, 442, 496
BspPI GGATC 3 cut(s) 272, 285, 808
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 1173
BsrFI RCCGGY 1 cut(s) 520
BsrI ACTGG 3 cut(s) 178, 273, 874
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 4 cut(s) 444, 483, 606, 1310
BssMI GATC 4 cut(s) 256, 277, 420, 800
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 2 cut(s) 810, 1311
Bst4CI ACNGT 1 cut(s) 406
Bst6I CTCTTC 2 cut(s) 1283, 1353
BstC8I GCNNGC 2 cut(s) 522, 624
BstDEI CTNAG 4 cut(s) 216, 378, 657, 882
BstF5I GGATG 1 cut(s) 948
BstHHI GCGC 1 cut(s) 996
BstKTI GATC 4 cut(s) 259, 280, 423, 803
BstMAI GTCTC 5 cut(s) 368, 389, 391, 1207, 1285
BstMBI GATC 4 cut(s) 256, 277, 420, 800
BstMWI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 662, 767, 1342
BstNI CCWGG 2 cut(s) 810, 1311
BstSCI CCNGG 2 cut(s) 808, 1309
BstSLI GKGCMC 2 cut(s) 178, 1347
BstV1I GCAGC 7 cut(s) 133, 136, 139, 665, 724, 1036, 1175
BstV2I GAAGAC 2 cut(s) 126, 1101
BstX2I RGATCY 2 cut(s) 277, 800
BstXI CCANNNNNNTGG 2 cut(s) 505, 673
BstYI RGATCY 2 cut(s) 277, 800
BsuRI GGCC 5 cut(s) 102, 482, 488, 520, 665
BtsCI GGATG 1 cut(s) 948
BtsIMutI CAGTG 3 cut(s) 624, 1086, 1108
Cac8I GCNNGC 2 cut(s) 522, 624
CfoI GCGC 1 cut(s) 996
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 181, 1156
CviAII CATG 5 cut(s) 371, 431, 1065, 1278, 1355
CviQI GTAC 2 cut(s) 181, 1156
DdeI CTNAG 4 cut(s) 216, 378, 657, 882
DpnI GATC 4 cut(s) 258, 279, 422, 802
DpnII GATC 4 cut(s) 256, 277, 420, 800
DrdI GACNNNNNNGTC 1 cut(s) 1268
DriI GACNNNNNGTC 1 cut(s) 408
DseDI GACNNNNNNGTC 1 cut(s) 1268
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 2 cut(s) 1283, 1353
Eam1105I GACNNNNNGTC 1 cut(s) 408
EarI CTCTTC 2 cut(s) 1283, 1353
Eco130I CCWWGG 1 cut(s) 606
Eco31I GGTCTC 1 cut(s) 389
Eco32I GATATC 1 cut(s) 1225
Eco57I CTGAAG 1 cut(s) 141
EcoRII CCWGG 2 cut(s) 808, 1309
EcoRV GATATC 1 cut(s) 1225
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 1 cut(s) 1066
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 5 cut(s) 374, 434, 1068, 1281, 1358
FalI AAGNNNNNCTT 4 cut(s) 70, 102, 962, 994
FaqI GGGAC 2 cut(s) 1218, 1256
FatI CATG 5 cut(s) 370, 430, 1064, 1277, 1354
FauI CCCGC 1 cut(s) 7
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
FokI GGATG 1 cut(s) 955
Fsp4HI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 995
GluI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
GsaI CCCAGC 2 cut(s) 252, 761
HaeIII GGCC 5 cut(s) 102, 482, 488, 520, 665
HapII CCGG 5 cut(s) 140, 171, 186, 521, 725
HhaI GCGC 1 cut(s) 996
Hin1II CATG 5 cut(s) 374, 434, 1068, 1281, 1358
Hin6I GCGC 1 cut(s) 994
HinP1I GCGC 1 cut(s) 994
HincII GTYRAC 2 cut(s) 534, 793
HindII GTYRAC 2 cut(s) 534, 793
HindIII AAGCTT 2 cut(s) 79, 284
HinfI GANTC 9 cut(s) 66, 581, 595, 707, 862, 1073, 1190, 1215, 1418
HpaII CCGG 5 cut(s) 140, 171, 186, 521, 725
HphI GGTGA 2 cut(s) 946, 1265
Hpy166II GTNNAC 3 cut(s) 534, 793, 1158
Hpy188I TCNGA 2 cut(s) 1189, 1242
Hpy188III TCNNGA 3 cut(s) 90, 260, 570
Hpy8I GTNNAC 3 cut(s) 534, 793, 1158
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 5 cut(s) 229, 358, 655, 781, 1123
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 2 cut(s) 120, 730
HpyCH4V TGCA 4 cut(s) 39, 370, 626, 1064
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 662, 767, 1342
HpyF3I CTNAG 4 cut(s) 216, 378, 657, 882
HpySE526I ACGT 2 cut(s) 120, 730
Hsp92II CATG 5 cut(s) 374, 434, 1068, 1281, 1358
HspAI GCGC 1 cut(s) 994
KroI GCCGGC 1 cut(s) 520
KroNI GCCGGC 1 cut(s) 522
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 4 cut(s) 256, 277, 420, 800
LmnI GCTCC 4 cut(s) 6, 173, 440, 1429
Lsp1109I GCAGC 7 cut(s) 133, 136, 139, 665, 724, 1036, 1175
MaeI CTAG 1 cut(s) 540
MaeII ACGT 2 cut(s) 120, 730
MaeIII GTNAC 3 cut(s) 426, 1084, 1246
MalI GATC 4 cut(s) 258, 279, 422, 802
MboI GATC 4 cut(s) 256, 277, 420, 800
MfeI CAATTG 1 cut(s) 359
MflI RGATCY 2 cut(s) 277, 800
MhlI GDGCHC 2 cut(s) 178, 1347
MlyI GAGTC 4 cut(s) 60, 716, 1067, 1209
MnlI CCTC 7 cut(s) 214, 219, 232, 268, 454, 478, 1116
Mph1103I ATGCAT 1 cut(s) 1066
MroNI GCCGGC 1 cut(s) 520
MseI TTAA 2 cut(s) 57, 1389
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 2 cut(s) 656, 686
MspI CCGG 5 cut(s) 140, 171, 186, 521, 725
MspR9I CCNGG 2 cut(s) 810, 1311
MunI CAATTG 1 cut(s) 359
Mva1269I GAATGC 3 cut(s) 679, 1066, 1288
MvaI CCWGG 2 cut(s) 810, 1311
MwoI GCNNNNNNNGC 6 cut(s) 60, 152, 208, 662, 767, 1342
NaeI GCCGGC 1 cut(s) 522
NdeII GATC 4 cut(s) 256, 277, 420, 800
NgoMIV GCCGGC 1 cut(s) 520
NlaIII CATG 5 cut(s) 374, 434, 1068, 1281, 1358
NlaIV GGNNCC 5 cut(s) 169, 175, 279, 442, 496
NmeAIII GCCGAG 2 cut(s) 57, 508
NmuCI GTSAC 1 cut(s) 1084
NsiI ATGCAT 1 cut(s) 1066
PctI GAATGC 3 cut(s) 679, 1066, 1288
PdiI GCCGGC 1 cut(s) 522
PfeI GAWTC 5 cut(s) 581, 595, 862, 1190, 1418
PkrI GCNGC 8 cut(s) 148, 151, 154, 167, 655, 739, 1051, 1165
PleI GAGTC 4 cut(s) 60, 715, 1067, 1209
PpsI GAGTC 4 cut(s) 60, 715, 1067, 1209
Psp6I CCWGG 2 cut(s) 808, 1309
PspFI CCCAGC 2 cut(s) 248, 757
PspGI CCWGG 2 cut(s) 808, 1309
PspN4I GGNNCC 5 cut(s) 169, 175, 279, 442, 496
PspPI GGNCC 1 cut(s) 101
PsuI RGATCY 2 cut(s) 277, 800
PvuII CAGCTG 2 cut(s) 656, 686
RsaI GTAC 2 cut(s) 182, 1157
RsaNI GTAC 2 cut(s) 181, 1156
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 2 cut(s) 57, 1389
SatI GCNGC 8 cut(s) 147, 150, 153, 166, 654, 738, 1050, 1164
Sau3AI GATC 4 cut(s) 256, 277, 420, 800
Sau96I GGNCC 1 cut(s) 101
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 4 cut(s) 60, 716, 1067, 1209
ScrFI CCNGG 2 cut(s) 810, 1311
SduI GDGCHC 2 cut(s) 178, 1347
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 2 cut(s) 76, 388
SmoI CTYRAG 2 cut(s) 76, 388
SsiI CCGC 4 cut(s) 14, 165, 1407, 1424
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 2 cut(s) 808, 1309
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 2 cut(s) 123, 733
TaqI TCGA 3 cut(s) 50, 89, 1076
TaqII GACCGA 1 cut(s) 1278
TatI WGTACW 2 cut(s) 180, 1155
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 5 cut(s) 581, 595, 862, 1190, 1418
Tru1I TTAA 2 cut(s) 57, 1389
Tru9I TTAA 2 cut(s) 57, 1389
TscAI CASTG 3 cut(s) 624, 1093, 1108
TseFI GTSAC 1 cut(s) 1084
TseI GCWGC 7 cut(s) 146, 149, 152, 653, 737, 1049, 1163
Tsp45I GTSAC 1 cut(s) 1084
TspDTI ATGAA 7 cut(s) 279, 366, 485, 628, 1107, 1173, 1371
TspGWI ACGGA 2 cut(s) 588, 1208
TspRI CASTG 3 cut(s) 624, 1093, 1108
XapI RAATTY 5 cut(s) 844, 896, 974, 1037, 1394
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.