Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004027

Physical Location & Seq

Genomic Coordinates Reverse (-)

3736578 .. 3738208

Gene Structure 1631 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G11840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1371 bp

ATGGATCAAGAAGAGGAAGAGATCGAGATTCCTAATTACTTTATCTGCCCAATCTCACTAGAGATCATGAAAGATCCAGTCACGACCGTTTCTGGCATCACTTACGACCGTCAAAACATCGTTAAATGGCTCGAAAAGGTCCCTTCTTGTCCCGTCACGAAGCAACCTCTTCCCTTAGACTCCGATCTTACACCTAACCACATGCTTCGCCGTCTTATCCAACACTGGTGCGTCGAGAACGAGACACGTGGCGTTGTCAGAATCTCTACACCTCGAGTTCCTCCTGGAAAACTTAACGTCGTCGAAGAGATCAAGAATCTCAAAAAGTTCGGCCAAGAAGCCTTAGGAAGAGAAGAAACGTTGCAGAAGCTTGAAGTTTTGGCAATGGACGGAAATAACAGAAGATTGATGTGTGAATGTGGCGTACATAAGTCTTTGATCTTGTTCGTAGTCAAGTGTACTAGTGAAGACGAAGACGGACGACGTCGTATCAAAGGACTAGATGAGTCTCTTCGTCTTCTTCACTTGATCGGTATTCCATCAAACGACGCAAAAACAATCTTGATGGAAAACGATCGAGTTATGGAATCGTTGACTTGGGTTTTACACCAAGAAGACTTTCTCAGTAAGGCTTATACAATTGTGCTATTGAGGAATCTAACGGAATACACTTCCTCTCATATCGTAGAGAGATTGAATCCCGAGATTTTTAAAGGGATCATAGGGTTTCTCAAGGATGTTGTAAATAGTGTTAATCGGACGAGCCCTACCGTGAGAGAAACGGTACAGTCATCATCAAGGCCTAGCTTGGGAAAAACGGAACCATCAAAACTTGATCATAGTTTGGTCATCAAACAAGCGGTGACTGCCGCGCTGATGATTCTTTTAGAGACTTCTTCGTGGAGCCGAAACCGATCACTCCTTGTGGATCTCGGTGCGGTTTCTGAACTCATCGAACTCGAAATAAGCTACACTGGCGAGAAAAGAATCACAGAACTTATGTTAGGAGTTTTATCTCGTTTATGTTGCTGTGCGAACGGTAGAGCCGAGATTCTTGCTCATAGGGGCGGAATCGCGGTTGTGACAAAGCGGCTATTGAGGGTTTCACCTGCGGCAGACGATAGAGCTATCTCTATCCTAACTACAGTGTCAAAATTCTCGCCGGAAAACATGGTGGTGGAAGAGATGGTTAATGTTGGGACGGTAGAGAAGCTCTGTTCCGTTCTAGGAATGGATTGTGGTTTGAACTTGAAGGAGAAAGCAAAAGAGATACTAAAAGATCATTTTGATGAGTGGAAGAAGTTTCCATGTATTGACATAACTCTTCTCACTAAGCTGCTAAGCATCTCACCCAAAGGGCCAAAGATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

456

Amino Acids

51.29

Weight (kDa)

6.55

Isoelectric Point (pI)

50.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	10 - 81	1.8e-21	U-box domain
ARM_PUB	PF25598	99 - 446	3.3e-99	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004027)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11840
fragaria_vesca	FvH4_3g26390 FvH4_6g20390
malus_domestica	MD11G1173500.v1.1 MD12G1040800.v1.1 MD14G1039900.v1.1
prunus_persica	Prupe.6G145200_v2.0.a1 Prupe.6G145200_v2.0.a1 Prupe.7G094100_v2.0.a1
pyrus_communis	pycom11g14800 pycom12g03490 pycom14g03410
rosa_chinensis	RchiOBHm_Chr3g0476021 RchiOBHm_Chr5g0048571
rosa_laevigata	RLG00000023791 RLG00000034535
rosa_multiflora	Rmu_sc0000585.1_g000008 Rmu_sc0007341.1_g000034
rosa_roxburghii	Rroxscaffold_1G00033260 Rroxscaffold_6G00405500
rosa_rugosa	Rorug03G0152800 Rorug05G0239500
rosa_samantha	Rh3AG203700 Rh3BG233600 Rh3CG229000 Rh3DG229100 Rh5AG319200 Rh5BG329600 Rh5CG355500 Rh5DG340800
rosa_wichuraiana	Rw0G021240 Rw3G018510 Rw5G029850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1117
AatII	GACGTC	1 cut(s)	487
Acc36I	ACCTGC	1 cut(s)	1117
AccII	CGCG	2 cut(s)	872, 1076
AciI	CCGC	7 cut(s)	860, 870, 938, 1068, 1076, 1090, 1112
AclI	AACGTT	1 cut(s)	359
AclWI	GGATC	4 cut(s)	12, 68, 725, 936
AcoI	YGGCCR	1 cut(s)	331
AcsI	RAATTY	1 cut(s)	1154
AcvI	CACGTG	1 cut(s)	248
AcyI	GRCGYC	1 cut(s)	484
AfaI	GTAC	3 cut(s)	426, 460, 786
AfiI	CCNNNNNNNGG	1 cut(s)	809
AflIII	ACRYGT	1 cut(s)	245
AgsI	TTSAA	4 cut(s)	374, 697, 1246, 1252
AhlI	ACTAGT	1 cut(s)	461
AjnI	CCWGG	1 cut(s)	283
AjuI	GAANNNNNNNTTGG	2 cut(s)	603, 635
AluBI	AGCT	6 cut(s)	370, 807, 969, 1127, 1213, 1336
AluI	AGCT	6 cut(s)	370, 807, 969, 1127, 1213, 1336
Alw26I	GTCTC	3 cut(s)	236, 513, 884
AlwI	GGATC	4 cut(s)	12, 68, 725, 936
Ama87I	CYCGRG	2 cut(s)	273, 701
AoxI	GGCC	3 cut(s)	331, 800, 1358
ApeKI	GCWGC	1 cut(s)	1336
ApoI	RAATTY	1 cut(s)	1154
Asp700I	GAANNNNTTC	1 cut(s)	618
AspLEI	GCGC	1 cut(s)	874
AspS9I	GGNCC	2 cut(s)	139, 1358
AsuHPI	GGTGA	3 cut(s)	874, 1098, 1341
AvaI	CYCGRG	2 cut(s)	273, 701
AvaII	GGWCC	1 cut(s)	139
AxyI	CCTNAGG	1 cut(s)	343
BanII	GRGCYC	1 cut(s)	767
BbrPI	CACGTG	1 cut(s)	248
BbsI	GAAGAC	4 cut(s)	474, 480, 509, 621
BbvI	GCAGC	1 cut(s)	1323
BccI	CCATC	4 cut(s)	547, 559, 832, 1180
BceAI	ACGGC	1 cut(s)	195
BcgI	CGANNNNNNTGC	2 cut(s)	1037, 1071
BciT130I	CCWGG	1 cut(s)	285
BclI	TGATCA	1 cut(s)	835
BcoDI	GTCTC	3 cut(s)	236, 513, 884
BcuI	ACTAGT	1 cut(s)	461
BfaI	CTAG	5 cut(s)	59, 462, 500, 804, 1226
BfmI	CTRYAG	1 cut(s)	1143
BfuAI	ACCTGC	1 cut(s)	1117
BglII	AGATCT	1 cut(s)	1365
BisI	GCNGC	4 cut(s)	870, 1091, 1113, 1337
BlpI	GCTNAGC	1 cut(s)	1340
BlsI	GCNGC	4 cut(s)	871, 1092, 1114, 1338
Bme1390I	CCNGG	1 cut(s)	285
Bme18I	GGWCC	1 cut(s)	139
BmeT110I	CYCGRG	2 cut(s)	273, 701
BmgT120I	GGNCC	2 cut(s)	139, 1358
BmiI	GGNNCC	3 cut(s)	141, 822, 905
BmrFI	CCNGG	1 cut(s)	285
BmsI	GCATC	2 cut(s)	105, 1353
BpiI	GAAGAC	4 cut(s)	474, 480, 509, 621
Bpu1102I	GCTNAGC	1 cut(s)	1340
BpuEI	CTTGAG	1 cut(s)	716
BsaAI	YACGTR	1 cut(s)	248
BsaHI	GRCGYC	1 cut(s)	484
Bsc4I	CCNNNNNNNGG	1 cut(s)	809
Bse1I	ACTGG	3 cut(s)	77, 230, 979
Bse21I	CCTNAGG	1 cut(s)	343
Bse3DI	GCAATG	1 cut(s)	390
BseBI	CCWGG	1 cut(s)	285
BseGI	GGATG	1 cut(s)	742
BseLI	CCNNNNNNNGG	1 cut(s)	809
BseMI	GCAATG	1 cut(s)	390
BseMII	CTCAG	1 cut(s)	637
BseNI	ACTGG	3 cut(s)	77, 230, 979
BseXI	GCAGC	1 cut(s)	1323
Bsh1236I	CGCG	2 cut(s)	872, 1076
Bsh1285I	CGRYCG	3 cut(s)	87, 109, 577
BshFI	GGCC	3 cut(s)	333, 802, 1360
BsiEI	CGRYCG	3 cut(s)	87, 109, 577
BsiHKCI	CYCGRG	2 cut(s)	273, 701
BsiSI	CCGG	1 cut(s)	1163
BslFI	GGGAC	3 cut(s)	125, 135, 1213
BslI	CCNNNNNNNGG	1 cut(s)	809
BsmAI	GTCTC	3 cut(s)	236, 513, 884
BsmFI	GGGAC	3 cut(s)	125, 135, 1213
BsnI	GGCC	3 cut(s)	333, 802, 1360
BsoBI	CYCGRG	2 cut(s)	273, 701
Bsp1286I	GDGCHC	1 cut(s)	767
Bsp1720I	GCTNAGC	1 cut(s)	1340
BspACI	CCGC	7 cut(s)	860, 870, 938, 1068, 1076, 1090, 1112
BspANI	GGCC	3 cut(s)	333, 802, 1360
BspCNI	CTCAG	1 cut(s)	636
BspFNI	CGCG	2 cut(s)	872, 1076
BspHI	TCATGA	1 cut(s)	66
BspLI	GGNNCC	3 cut(s)	141, 822, 905
BspMI	ACCTGC	1 cut(s)	1117
BspPI	GGATC	4 cut(s)	12, 68, 725, 936
BsrDI	GCAATG	1 cut(s)	390
BsrI	ACTGG	3 cut(s)	77, 230, 979
BssNI	GRCGYC	1 cut(s)	484
Bst2UI	CCWGG	1 cut(s)	285
Bst4CI	ACNGT	8 cut(s)	88, 110, 772, 784, 789, 1040, 1147, 1204
Bst6I	CTCTTC	8 cut(s)	6, 12, 174, 300, 343, 516, 1176, 1329
BstACI	GRCGYC	1 cut(s)	484
BstBAI	YACGTR	1 cut(s)	248
BstDEI	CTNAG	5 cut(s)	175, 343, 623, 1332, 1340
BstF5I	GGATG	1 cut(s)	742
BstFNI	CGCG	2 cut(s)	872, 1076
BstHHI	GCGC	1 cut(s)	874
BstMAI	GTCTC	3 cut(s)	236, 513, 884
BstMCI	CGRYCG	3 cut(s)	87, 109, 577
BstMWI	GCNNNNNNNGC	2 cut(s)	866, 975
BstNI	CCWGG	1 cut(s)	285
BstNSI	RCATGY	1 cut(s)	205
BstSCI	CCNGG	1 cut(s)	283
BstSFI	CTRYAG	1 cut(s)	1143
BstUI	CGCG	2 cut(s)	872, 1076
BstV1I	GCAGC	1 cut(s)	1323
BstV2I	GAAGAC	4 cut(s)	474, 480, 509, 621
BstX2I	RGATCY	3 cut(s)	73, 928, 1365
BstYI	RGATCY	3 cut(s)	73, 928, 1365
Bsu36I	CCTNAGG	1 cut(s)	343
BsuRI	GGCC	3 cut(s)	333, 802, 1360
BtsCI	GGATG	1 cut(s)	742
BtsIMutI	CAGTG	3 cut(s)	223, 972, 1152
BveI	ACCTGC	1 cut(s)	1117
CciI	TCATGA	1 cut(s)	66
CfoI	GCGC	1 cut(s)	874
Cfr13I	GGNCC	2 cut(s)	139, 1358
CseI	GACGC	2 cut(s)	220, 557
Csp6I	GTAC	3 cut(s)	425, 459, 785
CviAII	CATG	4 cut(s)	67, 202, 1171, 1308
CviQI	GTAC	3 cut(s)	425, 459, 785
DdeI	CTNAG	5 cut(s)	175, 343, 623, 1332, 1340
DraI	TTTAAA	1 cut(s)	712
EaeI	YGGCCR	1 cut(s)	331
Eam1104I	CTCTTC	8 cut(s)	6, 12, 174, 300, 343, 516, 1176, 1329
EarI	CTCTTC	8 cut(s)	6, 12, 174, 300, 343, 516, 1176, 1329
EciI	GGCGGA	1 cut(s)	1083
Eco147I	AGGCCT	1 cut(s)	802
Eco24I	GRGCYC	1 cut(s)	767
Eco47I	GGWCC	1 cut(s)	139
Eco72I	CACGTG	1 cut(s)	248
Eco81I	CCTNAGG	1 cut(s)	343
Eco88I	CYCGRG	2 cut(s)	273, 701
EcoO109I	RGGNCCY	1 cut(s)	139
EcoRII	CCWGG	1 cut(s)	283
EcoT38I	GRGCYC	1 cut(s)	767
FaeI	CATG	4 cut(s)	70, 205, 1174, 1311
FaqI	GGGAC	3 cut(s)	125, 135, 1213
FatI	CATG	4 cut(s)	66, 201, 1170, 1307
FbaI	TGATCA	1 cut(s)	835
Fnu4HI	GCNGC	4 cut(s)	870, 1091, 1113, 1337
FokI	GGATG	1 cut(s)	749
FriOI	GRGCYC	1 cut(s)	767
Fsp4HI	GCNGC	4 cut(s)	870, 1091, 1113, 1337
FspBI	CTAG	5 cut(s)	59, 462, 500, 804, 1226
GlaI	GCGC	1 cut(s)	873
GluI	GCNGC	4 cut(s)	870, 1091, 1113, 1337
HaeIII	GGCC	3 cut(s)	333, 802, 1360
HapII	CCGG	1 cut(s)	1163
HgaI	GACGC	2 cut(s)	220, 557
HhaI	GCGC	1 cut(s)	874
Hin1I	GRCGYC	1 cut(s)	484
Hin1II	CATG	4 cut(s)	70, 205, 1174, 1311
Hin6I	GCGC	1 cut(s)	872
HinP1I	GCGC	1 cut(s)	872
HincII	GTYRAC	1 cut(s)	594
HindII	GTYRAC	1 cut(s)	594
HindIII	AAGCTT	1 cut(s)	368
HpaII	CCGG	1 cut(s)	1163
HphI	GGTGA	3 cut(s)	874, 1098, 1341
Hpy166II	GTNNAC	2 cut(s)	459, 594
Hpy188I	TCNGA	4 cut(s)	184, 260, 759, 946
Hpy188III	TCNNGA	9 cut(s)	8, 25, 67, 82, 157, 235, 313, 562, 701
Hpy8I	GTNNAC	2 cut(s)	459, 594
Hpy99I	CGWCG	6 cut(s)	236, 302, 305, 486, 489, 551
HpyAV	CCTTC	2 cut(s)	153, 1246
HpyCH4III	ACNGT	8 cut(s)	88, 110, 772, 784, 789, 1040, 1147, 1204
HpyCH4IV	ACGT	4 cut(s)	247, 297, 359, 484
HpyCH4V	TGCA	1 cut(s)	364
HpyF10VI	GCNNNNNNNGC	2 cut(s)	866, 975
HpyF3I	CTNAG	5 cut(s)	175, 343, 623, 1332, 1340
HpySE526I	ACGT	4 cut(s)	247, 297, 359, 484
Hsp92I	GRCGYC	1 cut(s)	484
Hsp92II	CATG	4 cut(s)	70, 205, 1174, 1311
HspAI	GCGC	1 cut(s)	872
Ksp22I	TGATCA	1 cut(s)	835
LmnI	GCTCC	1 cut(s)	903
LpnPI	CCDG	8 cut(s)	78, 90, 211, 270, 297, 960, 1122, 1176
Lsp1109I	GCAGC	1 cut(s)	1323
LweI	GCATC	2 cut(s)	105, 1353
MaeI	CTAG	5 cut(s)	59, 462, 500, 804, 1226
MaeII	ACGT	4 cut(s)	247, 297, 359, 484
MaeIII	GTNAC	4 cut(s)	79, 154, 862, 1081
MfeI	CAATTG	1 cut(s)	639
MflI	RGATCY	3 cut(s)	73, 928, 1365
MhlI	GDGCHC	1 cut(s)	767
MluCI	AATT	3 cut(s)	34, 639, 1154
MlyI	GAGTC	2 cut(s)	173, 515
MmeI	TCCRAC	1 cut(s)	244
MnlI	CCTC	7 cut(s)	7, 177, 282, 291, 645, 685, 1092
MroXI	GAANNNNTTC	1 cut(s)	618
MseI	TTAA	5 cut(s)	123, 294, 711, 753, 1191
MslI	CAYNNNNRTG	2 cut(s)	1175, 1287
MspI	CCGG	1 cut(s)	1163
MspR9I	CCNGG	1 cut(s)	285
MunI	CAATTG	1 cut(s)	639
MvaI	CCWGG	1 cut(s)	285
MvnI	CGCG	2 cut(s)	872, 1076
MwoI	GCNNNNNNNGC	2 cut(s)	866, 975
NlaIII	CATG	4 cut(s)	70, 205, 1174, 1311
NlaIV	GGNNCC	3 cut(s)	141, 822, 905
NmeAIII	GCCGAG	1 cut(s)	1072
NmuCI	GTSAC	4 cut(s)	79, 154, 862, 1081
NspI	RCATGY	1 cut(s)	205
PaeR7I	CTCGAG	1 cut(s)	273
PagI	TCATGA	1 cut(s)	66
PaqCI	CACCTGC	1 cut(s)	1117
PceI	AGGCCT	1 cut(s)	802
PcsI	WCGNNNNNNNCGW	1 cut(s)	1044
PdmI	GAANNNNTTC	1 cut(s)	618
PflFI	GACNNNGTC	1 cut(s)	483
PfoI	TCCNGGA	1 cut(s)	283
PkrI	GCNGC	4 cut(s)	871, 1092, 1114, 1338
Ple19I	CGATCG	1 cut(s)	577
PleI	GAGTC	2 cut(s)	173, 514
PmaCI	CACGTG	1 cut(s)	248
PmlI	CACGTG	1 cut(s)	248
PpsI	GAGTC	2 cut(s)	173, 514
Ppu21I	YACGTR	1 cut(s)	248
PpuMI	RGGWCCY	1 cut(s)	139
Psp1406I	AACGTT	1 cut(s)	359
Psp5II	RGGWCCY	1 cut(s)	139
Psp6I	CCWGG	1 cut(s)	283
PspCI	CACGTG	1 cut(s)	248
PspGI	CCWGG	1 cut(s)	283
PspN4I	GGNNCC	3 cut(s)	141, 822, 905
PspPI	GGNCC	2 cut(s)	139, 1358
PspPPI	RGGWCCY	1 cut(s)	139
PspXI	VCTCGAGB	1 cut(s)	273
PsuI	RGATCY	3 cut(s)	73, 928, 1365
PsyI	GACNNNGTC	1 cut(s)	483
PvuI	CGATCG	1 cut(s)	577
RsaI	GTAC	3 cut(s)	426, 460, 786
RsaNI	GTAC	3 cut(s)	425, 459, 785
RseI	CAYNNNNRTG	2 cut(s)	1175, 1287
SaqAI	TTAA	5 cut(s)	123, 294, 711, 753, 1191
SatI	GCNGC	4 cut(s)	870, 1091, 1113, 1337
Sau96I	GGNCC	2 cut(s)	139, 1358
SchI	GAGTC	2 cut(s)	173, 515
ScrFI	CCNGG	1 cut(s)	285
SduI	GDGCHC	1 cut(s)	767
SfaNI	GCATC	2 cut(s)	105, 1353
SfcI	CTRYAG	1 cut(s)	1143
Sfr274I	CTCGAG	1 cut(s)	273
SinI	GGWCC	1 cut(s)	139
SlaI	CTCGAG	1 cut(s)	273
SmiMI	CAYNNNNRTG	2 cut(s)	1175, 1287
SmlI	CTYRAG	2 cut(s)	273, 731
SmoI	CTYRAG	2 cut(s)	273, 731
SpeI	ACTAGT	1 cut(s)	461
Sse9I	AATT	3 cut(s)	34, 639, 1154
SseBI	AGGCCT	1 cut(s)	802
SsiI	CCGC	7 cut(s)	860, 870, 938, 1068, 1076, 1090, 1112
SspMI	CTAG	5 cut(s)	59, 462, 500, 804, 1226
StuI	AGGCCT	1 cut(s)	802
StyD4I	CCNGG	1 cut(s)	283
TaaI	ACNGT	8 cut(s)	88, 110, 772, 784, 789, 1040, 1147, 1204
TaiI	ACGT	4 cut(s)	250, 300, 362, 487
TaqI	TCGA	8 cut(s)	24, 132, 234, 274, 303, 577, 954, 960
TasI	AATT	3 cut(s)	34, 639, 1154
TatI	WGTACW	1 cut(s)	458
TauI	GCSGC	3 cut(s)	872, 1093, 1115
Tru1I	TTAA	5 cut(s)	123, 294, 711, 753, 1191
Tru9I	TTAA	5 cut(s)	123, 294, 711, 753, 1191
TscAI	CASTG	3 cut(s)	230, 979, 1152
TseFI	GTSAC	4 cut(s)	79, 154, 862, 1081
TseI	GCWGC	1 cut(s)	1336
Tsp45I	GTSAC	4 cut(s)	79, 154, 862, 1081
TspDTI	ATGAA	1 cut(s)	83
TspGWI	ACGGA	5 cut(s)	405, 492, 677, 833, 1210
TspRI	CASTG	3 cut(s)	230, 979, 1152
Tth111I	GACNNNGTC	1 cut(s)	483
VpaK11BI	GGWCC	1 cut(s)	139
XapI	RAATTY	1 cut(s)	1154
XceI	RCATGY	1 cut(s)	205
XhoI	CTCGAG	1 cut(s)	273
XmnI	GAANNNNTTC	1 cut(s)	618
XspI	CTAG	5 cut(s)	59, 462, 500, 804, 1226
ZraI	GACGTC	1 cut(s)	485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.