Rorug05G0239500

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004027
Physical Location & Seq
Reverse (-)
25386546 .. 25389844
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0239500.1

Sequence Viewer

Length: 1185 bp
ATGGCGCTAGAGTTCAGGTCCCACAAGGACGACGCCTGGTACGACGCCCGCCTATTCACGGAGGACACCGGCGCCGGTCTCCGCCTCAGAATCACCTTCGCCAACTTCTCCGAGGACCACGACGAGTTCGTCGGCGCCAACGACCTCGCCGCCCTCCCAGACCTCGACGCATTGCGATCCCGCGTCCGCCGCCTCTCCCTCCAGCTCCAGGACTCCGAGTGCTCCTCCGTCGACAGAGGCCTCCTCGTCTGCGCCGCCCGCTCCGTCCACCCCGACGACCGCCGCTTCTTCGACGCCTTCGTCGACGGGGTTGTGCATAAGGAGCATGGGCAGGAGGAGTGCACATGCAGCTTTGTTCTGGAGTGGATTCACGGCCCGCAAGTTGGGACTCTCAGTGTGGTGGCGCTTGAGAATATTTGCAGAGTCCGGCTGCCGGCGGTGGACGAAGAGATTGACCCGGCGTTGACCTCTTTTGTTAACGCGGTCAAGGAGAAGATGATTGAGAATTCTGGGGAGAAAGGTGGTGGTGGTGATTGTGACAATGAGTCTAGTAGTAGGCACAGGAAGAGGCACAAGGGAAGCTCTTCTCAGCTGAACAAGGATACAAGCCGTGTCAGGCAATCCTTAACTCCAAGCTCGTCTTCCAAAGAGATAATGGACAATATTTCTGCAAGGGATGGACATGACATGGATATTGGAGGGCTTCCCCATATGATCTTTGTTGAGAATCTTGAGAAGGGGATCTCATCATTTACAATTATGGAATTCATATACCATCGACTTTCAATATCATGTCAAGCATTTGTTTCTCCAAGTAAGCAATCAGAGGTTTATACAAGAGGAACCATCATGTTGAACAGCAAAAAGAACCTCGACAAGTTATCTGAATTTTTGGGAAGTCCAGATCGCATCATCATCTCGTCAACAGGAAGGCCTTGGGTTCTGACTGAGAAAATTCCCATGGATGGCACAATAAGGGCATCGATTGAAACCTTTAAGCGTACATCTCAGGCAACATTAGAGTGTAAAACTCTAAGTAGGAATGAGGTGAAGGTTGTAATGTCTGGGACTCCAGAATACACTAAAGCAAAGCAGCTGAAGGATTTGTTCGAGGAGTTTACTAATCATCAGTGTGGACTTCATCAGAGGTTACTCCAGGAAGAGGGAAAAATCATATGCGGCTAG

Protein Analysis

394

Amino Acids

44.1

Weight (kDa)

5.85

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAWADEE PF16719 2 - 140 3.8e-34 SAWADEE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 128, 299
AccB1I GGYRCC 2 cut(s) 71, 134
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 2 cut(s) 231, 303
AccII CGCG 2 cut(s) 183, 482
AclWI GGATC 2 cut(s) 171, 749
AcsI RAATTY 4 cut(s) 505, 764, 887, 954
AcuI CTGAAG 1 cut(s) 1118
AcyI GRCGYC 5 cut(s) 33, 45, 72, 135, 294
AfaI GTAC 2 cut(s) 41, 1003
AfiI CCNNNNNNNGG 6 cut(s) 58, 208, 383, 433, 965, 1162
AgsI TTSAA 3 cut(s) 786, 856, 989
AhdI GACNNNNNGTC 1 cut(s) 544
AjnI CCWGG 3 cut(s) 35, 207, 1155
AjuI GAANNNNNNNTTGG 2 cut(s) 625, 657
AluBI AGCT 6 cut(s) 205, 351, 582, 592, 636, 1096
AluI AGCT 6 cut(s) 205, 351, 582, 592, 636, 1096
Alw21I GWGCWC 2 cut(s) 224, 344
Alw26I GTCTC 1 cut(s) 83
Alw44I GTGCAC 1 cut(s) 340
AlwI GGATC 2 cut(s) 171, 749
AoxI GGCC 3 cut(s) 238, 373, 932
ApaLI GTGCAC 1 cut(s) 340
ApeKI GCWGC 3 cut(s) 348, 430, 1093
ApoI RAATTY 4 cut(s) 505, 764, 887, 954
Asp700I GAANNNNTTC 1 cut(s) 583
AspLEI GCGC 5 cut(s) 7, 74, 137, 254, 406
AspS9I GGNCC 3 cut(s) 18, 115, 374
AsuC2I CCSGG 1 cut(s) 458
AsuHPI GGTGA 3 cut(s) 85, 542, 1060
AvaII GGWCC 2 cut(s) 18, 115
BaeGI GKGCMC 1 cut(s) 344
BanI GGYRCC 2 cut(s) 71, 134
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 2 cut(s) 224, 344
BbvI GCAGC 3 cut(s) 360, 417, 1105
BccI CCATC 4 cut(s) 671, 783, 854, 959
BceAI ACGGC 2 cut(s) 388, 594
BciT130I CCWGG 3 cut(s) 37, 209, 1157
BciVI GTATCC 1 cut(s) 595
BcnI CCSGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 3 cut(s) 8, 549, 1183
BfoI RGCGCY 4 cut(s) 8, 75, 138, 407
BfuI GTATCC 1 cut(s) 595
BisI GCNGC 8 cut(s) 150, 190, 255, 283, 349, 431, 1094, 1180
BlsI GCNGC 8 cut(s) 151, 191, 256, 284, 350, 432, 1095, 1181
Bme1390I CCNGG 4 cut(s) 37, 209, 458, 1157
Bme18I GGWCC 2 cut(s) 18, 115
BmeRI GACNNNNNGTC 1 cut(s) 544
BmgT120I GGNCC 3 cut(s) 18, 115, 374
BmiI GGNNCC 4 cut(s) 20, 73, 136, 844
BmrFI CCNGG 4 cut(s) 37, 209, 458, 1157
BmsI GCATC 2 cut(s) 918, 989
BpiI GAAGAC 1 cut(s) 633
BplI GAGNNNNNCTC 4 cut(s) 209, 241, 228, 260
BpmI CTGGAG 5 cut(s) 185, 191, 380, 1056, 1139
BpuEI CTTGAG 2 cut(s) 428, 752
BpuMI CCSGG 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 983
BsaHI GRCGYC 5 cut(s) 33, 45, 72, 135, 294
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 3 cut(s) 111, 935, 960
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 208, 383, 433, 965, 1162
Bse118I RCCGGY 3 cut(s) 68, 74, 433
Bse3DI GCAATG 1 cut(s) 170
BseBI CCWGG 3 cut(s) 37, 209, 1157
BseCI ATCGAT 1 cut(s) 983
BseDI CCNNGG 3 cut(s) 111, 935, 960
BseGI GGATG 2 cut(s) 682, 970
BseLI CCNNNNNNNGG 6 cut(s) 58, 208, 383, 433, 965, 1162
BseMI GCAATG 1 cut(s) 170
BseMII CTCAG 5 cut(s) 100, 406, 602, 939, 1022
BseRI GAGGAG 4 cut(s) 214, 233, 350, 1127
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 3 cut(s) 360, 417, 1105
Bsh1236I CGCG 2 cut(s) 183, 482
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 240, 375, 934
BshNI GGYRCC 2 cut(s) 71, 134
BshVI ATCGAT 1 cut(s) 983
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 2 cut(s) 224, 344
BsiSI CCGG 5 cut(s) 69, 75, 427, 434, 458
BslFI GGGAC 3 cut(s) 4, 400, 1081
BslI CCNNNNNNNGG 6 cut(s) 58, 208, 383, 433, 965, 1162
BsmAI GTCTC 1 cut(s) 83
BsmFI GGGAC 3 cut(s) 4, 400, 1081
BsnI GGCC 3 cut(s) 240, 375, 934
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 224, 344
Bsp143I GATC 4 cut(s) 176, 714, 741, 904
Bsp19I CCATGG 1 cut(s) 960
BspANI GGCC 3 cut(s) 240, 375, 934
BspCNI CTCAG 5 cut(s) 99, 405, 601, 940, 1021
BspDI ATCGAT 1 cut(s) 983
BspFNI CGCG 2 cut(s) 183, 482
BspLI GGNNCC 4 cut(s) 20, 73, 136, 844
BspPI GGATC 2 cut(s) 171, 749
BspQI GCTCTTC 1 cut(s) 589
BspT107I GGYRCC 2 cut(s) 71, 134
BspTNI GGTCTC 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 170
BsrFI RCCGGY 3 cut(s) 68, 74, 433
BssAI RCCGGY 3 cut(s) 68, 74, 433
BssECI CCNNGG 3 cut(s) 111, 935, 960
BssMI GATC 4 cut(s) 176, 714, 741, 904
BssNI GRCGYC 5 cut(s) 33, 45, 72, 135, 294
BssT1I CCWWGG 2 cut(s) 935, 960
Bst2UI CCWGG 3 cut(s) 37, 209, 1157
Bst6I CTCTTC 4 cut(s) 441, 560, 589, 1155
BstACI GRCGYC 5 cut(s) 33, 45, 72, 135, 294
BstC8I GCNNGC 4 cut(s) 49, 259, 377, 435
BstDEI CTNAG 6 cut(s) 86, 392, 588, 948, 1008, 1034
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 2 cut(s) 682, 970
BstFNI CGCG 2 cut(s) 183, 482
BstH2I RGCGCY 4 cut(s) 8, 75, 138, 407
BstHHI GCGC 5 cut(s) 7, 74, 137, 254, 406
BstKTI GATC 4 cut(s) 179, 717, 744, 907
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 4 cut(s) 176, 714, 741, 904
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 189, 258, 322, 348
BstNI CCWGG 3 cut(s) 37, 209, 1157
BstNSI RCATGY 1 cut(s) 348
BstSCI CCNGG 4 cut(s) 35, 207, 456, 1155
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 2 cut(s) 183, 482
BstV1I GCAGC 3 cut(s) 360, 417, 1105
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 1 cut(s) 741
BstYI RGATCY 1 cut(s) 741
Bsu15I ATCGAT 1 cut(s) 983
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 3 cut(s) 240, 375, 934
BsuTUI ATCGAT 1 cut(s) 983
BtgI CCRYGG 1 cut(s) 960
BtsCI GGATG 2 cut(s) 682, 970
BtsIMutI CAGTG 2 cut(s) 400, 1136
Cac8I GCNNGC 4 cut(s) 49, 259, 377, 435
CfoI GCGC 5 cut(s) 7, 74, 137, 254, 406
Cfr10I RCCGGY 3 cut(s) 68, 74, 433
Cfr13I GGNCC 3 cut(s) 18, 115, 374
ClaI ATCGAT 1 cut(s) 983
CseI GACGC 5 cut(s) 41, 53, 172, 176, 302
Csp6I GTAC 2 cut(s) 40, 1002
CviAII CATG 7 cut(s) 326, 345, 683, 688, 792, 850, 961
CviQI GTAC 2 cut(s) 40, 1002
DdeI CTNAG 6 cut(s) 86, 392, 588, 948, 1008, 1034
DinI GGCGCC 2 cut(s) 73, 136
DpnI GATC 4 cut(s) 178, 716, 743, 906
DpnII GATC 4 cut(s) 176, 714, 741, 904
DrdI GACNNNNNNGTC 2 cut(s) 128, 299
DriI GACNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 2 cut(s) 128, 299
Eam1104I CTCTTC 4 cut(s) 441, 560, 589, 1155
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 4 cut(s) 441, 560, 589, 1155
EciI GGCGGA 2 cut(s) 71, 176
Eco130I CCWWGG 2 cut(s) 935, 960
Eco147I AGGCCT 2 cut(s) 240, 934
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 18, 115
Eco57I CTGAAG 1 cut(s) 1118
EcoO109I RGGNCCY 1 cut(s) 18
EcoRI GAATTC 2 cut(s) 505, 764
EcoRII CCWGG 3 cut(s) 35, 207, 1155
EcoT14I CCWWGG 2 cut(s) 935, 960
EgeI GGCGCC 2 cut(s) 73, 136
EheI GGCGCC 2 cut(s) 73, 136
ErhI CCWWGG 2 cut(s) 935, 960
FaeI CATG 7 cut(s) 329, 348, 686, 691, 795, 853, 964
FalI AAGNNNNNCTT 2 cut(s) 625, 657
FaqI GGGAC 3 cut(s) 4, 400, 1081
FatI CATG 7 cut(s) 325, 344, 682, 687, 791, 849, 960
FauI CCCGC 4 cut(s) 56, 188, 266, 384
FauNDI CATATG 2 cut(s) 711, 1175
FblI GTMKAC 2 cut(s) 231, 303
Fnu4HI GCNGC 8 cut(s) 150, 190, 255, 283, 349, 431, 1094, 1180
FokI GGATG 2 cut(s) 689, 977
Fsp4HI GCNGC 8 cut(s) 150, 190, 255, 283, 349, 431, 1094, 1180
FspBI CTAG 3 cut(s) 8, 549, 1183
GlaI GCGC 5 cut(s) 6, 73, 136, 253, 405
GluI GCNGC 8 cut(s) 150, 190, 255, 283, 349, 431, 1094, 1180
GsuI CTGGAG 5 cut(s) 185, 191, 380, 1056, 1139
HaeII RGCGCY 4 cut(s) 8, 75, 138, 407
HaeIII GGCC 3 cut(s) 240, 375, 934
HapII CCGG 5 cut(s) 69, 75, 427, 434, 458
HgaI GACGC 5 cut(s) 41, 53, 172, 176, 302
HhaI GCGC 5 cut(s) 7, 74, 137, 254, 406
Hin1I GRCGYC 5 cut(s) 33, 45, 72, 135, 294
Hin1II CATG 7 cut(s) 329, 348, 686, 691, 795, 853, 964
Hin6I GCGC 5 cut(s) 5, 72, 135, 252, 404
HinP1I GCGC 5 cut(s) 5, 72, 135, 252, 404
HincII GTYRAC 5 cut(s) 232, 304, 465, 478, 924
HindII GTYRAC 5 cut(s) 232, 304, 465, 478, 924
HinfI GANTC 8 cut(s) 90, 212, 367, 388, 423, 545, 727, 1069
HpaI GTTAAC 1 cut(s) 478
HpaII CCGG 5 cut(s) 69, 75, 427, 434, 458
HphI GGTGA 3 cut(s) 85, 542, 1060
Hpy188I TCNGA 7 cut(s) 89, 112, 217, 826, 886, 945, 1146
Hpy188III TCNNGA 4 cut(s) 359, 731, 902, 1073
HpyAV CCTTC 6 cut(s) 106, 307, 730, 924, 1045, 1093
HpyCH4V TGCA 5 cut(s) 316, 342, 348, 420, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 258, 322, 348
HpyF3I CTNAG 6 cut(s) 86, 392, 588, 948, 1008, 1034
Hsp92I GRCGYC 5 cut(s) 33, 45, 72, 135, 294
Hsp92II CATG 7 cut(s) 329, 348, 686, 691, 795, 853, 964
HspAI GCGC 5 cut(s) 5, 72, 135, 252, 404
KasI GGCGCC 2 cut(s) 71, 134
KroI GCCGGC 1 cut(s) 433
KroNI GCCGGC 1 cut(s) 435
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 4 cut(s) 176, 714, 741, 904
LguI GCTCTTC 1 cut(s) 589
LmnI GCTCC 4 cut(s) 210, 227, 266, 322
Lsp1109I GCAGC 3 cut(s) 360, 417, 1105
LweI GCATC 2 cut(s) 918, 989
MaeI CTAG 3 cut(s) 8, 549, 1183
MaeIII GTNAC 2 cut(s) 536, 1149
MalI GATC 4 cut(s) 178, 716, 743, 906
MbiI CCGCTC 1 cut(s) 261
MboI GATC 4 cut(s) 176, 714, 741, 904
MboII GAAGA 7 cut(s) 280, 458, 505, 576, 577, 633, 1172
MflI RGATCY 1 cut(s) 741
MhlI GDGCHC 2 cut(s) 224, 344
MluCI AATT 5 cut(s) 505, 756, 764, 887, 954
Mly113I GGCGCC 2 cut(s) 72, 135
MlyI GAGTC 5 cut(s) 206, 382, 432, 554, 1063
MroNI GCCGGC 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 583
MseI TTAA 3 cut(s) 477, 626, 996
MslI CAYNNNNRTG 2 cut(s) 1021, 1131
MspA1I CMGCKG 2 cut(s) 592, 1096
MspI CCGG 5 cut(s) 69, 75, 427, 434, 458
MspR9I CCNGG 4 cut(s) 37, 209, 458, 1157
MvaI CCWGG 3 cut(s) 37, 209, 1157
MvnI CGCG 2 cut(s) 183, 482
MwoI GCNNNNNNNGC 4 cut(s) 189, 258, 322, 348
NaeI GCCGGC 1 cut(s) 435
NarI GGCGCC 2 cut(s) 72, 135
NciI CCSGG 1 cut(s) 458
NcoI CCATGG 1 cut(s) 960
NdeI CATATG 2 cut(s) 711, 1175
NdeII GATC 4 cut(s) 176, 714, 741, 904
NgoMIV GCCGGC 1 cut(s) 433
NlaIII CATG 7 cut(s) 329, 348, 686, 691, 795, 853, 964
NlaIV GGNNCC 4 cut(s) 20, 73, 136, 844
NmuCI GTSAC 1 cut(s) 536
NspI RCATGY 1 cut(s) 348
PceI AGGCCT 2 cut(s) 240, 934
PciSI GCTCTTC 1 cut(s) 589
PcsI WCGNNNNNNNCGW 5 cut(s) 39, 126, 138, 297, 300
PdiI GCCGGC 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 583
PfeI GAWTC 3 cut(s) 90, 367, 727
PfoI TCCNGGA 2 cut(s) 207, 1155
PkrI GCNGC 8 cut(s) 151, 191, 256, 284, 350, 432, 1095, 1181
PleI GAGTC 5 cut(s) 206, 382, 431, 553, 1063
PluTI GGCGCC 2 cut(s) 75, 138
PpsI GAGTC 5 cut(s) 206, 382, 431, 553, 1063
PpuMI RGGWCCY 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 35, 207, 1155
PspGI CCWGG 3 cut(s) 35, 207, 1155
PspN4I GGNNCC 4 cut(s) 20, 73, 136, 844
PspPI GGNCC 3 cut(s) 18, 115, 374
PspPPI RGGWCCY 1 cut(s) 18
PsuI RGATCY 1 cut(s) 741
PvuII CAGCTG 2 cut(s) 592, 1096
RsaI GTAC 2 cut(s) 41, 1003
RsaNI GTAC 2 cut(s) 40, 1002
RseI CAYNNNNRTG 2 cut(s) 1021, 1131
SalI GTCGAC 2 cut(s) 230, 302
SapI GCTCTTC 1 cut(s) 589
SaqAI TTAA 3 cut(s) 477, 626, 996
SatI GCNGC 8 cut(s) 150, 190, 255, 283, 349, 431, 1094, 1180
Sau3AI GATC 4 cut(s) 176, 714, 741, 904
Sau96I GGNCC 3 cut(s) 18, 115, 374
SchI GAGTC 5 cut(s) 206, 382, 432, 554, 1063
ScrFI CCNGG 4 cut(s) 37, 209, 458, 1157
SduI GDGCHC 2 cut(s) 224, 344
SfaNI GCATC 2 cut(s) 918, 989
SfoI GGCGCC 2 cut(s) 73, 136
SgrAI CRCCGGYG 1 cut(s) 68
SgrDI CGTCGACG 1 cut(s) 302
SinI GGWCC 2 cut(s) 18, 115
SmiMI CAYNNNNRTG 2 cut(s) 1021, 1131
SmlI CTYRAG 2 cut(s) 407, 731
SmoI CTYRAG 2 cut(s) 407, 731
Sse9I AATT 5 cut(s) 505, 756, 764, 887, 954
SseBI AGGCCT 2 cut(s) 240, 934
SspDI GGCGCC 2 cut(s) 71, 134
SspI AATATT 2 cut(s) 415, 664
SspMI CTAG 3 cut(s) 8, 549, 1183
StuI AGGCCT 2 cut(s) 240, 934
StyD4I CCNGG 4 cut(s) 35, 207, 456, 1155
StyI CCWWGG 2 cut(s) 935, 960
TaqI TCGA 8 cut(s) 165, 231, 291, 303, 778, 873, 983, 1110
TasI AATT 5 cut(s) 505, 756, 764, 887, 954
TauI GCSGC 5 cut(s) 152, 192, 257, 285, 1182
TfiI GAWTC 3 cut(s) 90, 367, 727
Tru1I TTAA 3 cut(s) 477, 626, 996
Tru9I TTAA 3 cut(s) 477, 626, 996
TscAI CASTG 2 cut(s) 400, 1136
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 3 cut(s) 348, 430, 1093
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 2 cut(s) 757, 1130
TspGWI ACGGA 3 cut(s) 74, 217, 253
TspRI CASTG 2 cut(s) 400, 1136
VneI GTGCAC 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 18, 115
XapI RAATTY 4 cut(s) 505, 764, 887, 954
XceI RCATGY 1 cut(s) 348
XcmI CCANNNNNNNNNTGG 1 cut(s) 652
XmiI GTMKAC 2 cut(s) 231, 303
XmnI GAANNNNTTC 1 cut(s) 583
XspI CTAG 3 cut(s) 8, 549, 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.