AT3G12620

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005979
Physical Location & Seq
Reverse (-)
4009270 .. 4011675
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12620.1

Sequence Viewer

Length: 1158 bp
ATGGTATCATCGGCAACTATATTGCGGATGGTAGCACCATGTTGGAGGCGACCTTCTGTTAAAGGAGATCATTCTACTAGAGACGCTAATGGTCGATGTGATGGTCTTCTTTGGTATAAAGATTCTGGTAACCATGTTGCTGGAGAGTTCTCTATGTCTGTTATTCAAGCTAATAATCTTCTCGAAGATCATAGCAAGCTTGAGTCTGGACCTGTTAGTATGTTTGATTCTGGTCCTCAAGCTACTTTTGTTGGTGTTTATGATGGTCATGGTGGTCCTGAAGCTGCCCGTTTCGTTAATAAACACCTCTTCGACAACATCAGGAAGTTTACATCAGAGAATCATGGAATGTCTGCCAATGTTATAACTAAAGCGTTTTTAGCTACGGAAGAAGATTTTCTTTCTCTTGTCCGGCGGCAGTGGCAGATTAAACCACAGATTGCTTCTGTTGGAGCTTGTTGTCTGGTAGGGATCATATGTAGTGGATTGTTGTATATTGCAAACGCTGGAGATTCTCGGGTTGTGCTAGGAAGACTGGAAAAAGCGTTTAAAATTGTCAAGGCCGTTCAGTTATCGTCTGAGCATAATGCAAGTCTTGAATCTGTGAGAGAGGAGTTGCGCTCGTTGCATCCAAATGATCCTCAGATTGTGGTCTTGAAGCACAAAGTTTGGCGTGTCAAAGGTATTATACAGGTTTCACGGTCGATTGGTGATGCATACTTAAAGAAAGCAGAATTCAACCGGGAACCATTGTTGGCAAAGTTCAGGGTCCCTGAGGTTTTCCATAAGCCCATCCTTAGAGCAGAGCCGGCAATTACAGTACACAAAATCCATCCAGAAGATCAGTTTCTTATATTTGCATCAGACGGCTTGTGGGAGCACTTGTCCAATCAAGAAGCTGTTGACATTGTGAACACATGCCCGCGCAATGGGATTGCAAGGAAGCTCATAAAAACAGCTCTAAGAGAGGCAGCAAAGAAGAGAGAGATGAGATATTCGGATCTAAAAAAGATCGATAGAGGAGTGCGGAGACATTTTCATGATGATATAACAGTCATAGTTGTGTTTCTTGATTCACATCTCGTCAGTCGCAGTACCTCACGCCGACCTCTCCTCTCCATCTCAGGTGGTGGTGATTTAGCTGGACCTTCTACTTGA

Protein Analysis

385

Amino Acids

42.85

Weight (kDa)

9.39

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 76 - 326 5.6e-41 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 365
AccII CGCG 1 cut(s) 925
AciI CCGC 4 cut(s) 25, 415, 923, 1027
AclWI GGATC 3 cut(s) 479, 632, 1008
AcsI RAATTY 1 cut(s) 734
AcuI CTGAAG 1 cut(s) 300
AfaI GTAC 2 cut(s) 822, 1096
AfiI CCNNNNNNNGG 1 cut(s) 929
AgsI TTSAA 4 cut(s) 167, 599, 658, 739
Alw21I GWGCWC 1 cut(s) 882
Alw26I GTCTC 2 cut(s) 75, 1024
AlwI GGATC 3 cut(s) 479, 632, 1008
Ama87I CYCGRG 1 cut(s) 516
AoxI GGCC 1 cut(s) 561
ApeKI GCWGC 2 cut(s) 284, 971
ApoI RAATTY 1 cut(s) 734
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 2 cut(s) 621, 927
AspS9I GGNCC 5 cut(s) 209, 233, 275, 769, 1145
AsuC2I CCSGG 1 cut(s) 743
AsuHPI GGTGA 2 cut(s) 722, 1145
AvaI CYCGRG 1 cut(s) 516
AvaII GGWCC 5 cut(s) 209, 233, 275, 769, 1145
AxyI CCTNAGG 1 cut(s) 774
BbsI GAAGAC 2 cut(s) 98, 538
Bbv12I GWGCWC 1 cut(s) 882
BbvI GCAGC 2 cut(s) 271, 983
BccI CCATC 6 cut(s) 22, 95, 257, 800, 840, 1127
BceAI ACGGC 2 cut(s) 548, 883
BcnI CCSGG 1 cut(s) 743
BcoDI GTCTC 2 cut(s) 75, 1024
BfaI CTAG 2 cut(s) 78, 527
BisI GCNGC 3 cut(s) 285, 416, 972
BlsI GCNGC 3 cut(s) 286, 417, 973
Bme1390I CCNGG 1 cut(s) 743
Bme18I GGWCC 5 cut(s) 209, 233, 275, 769, 1145
BmeT110I CYCGRG 1 cut(s) 516
BmgT120I GGNCC 5 cut(s) 209, 233, 275, 769, 1145
BmiI GGNNCC 3 cut(s) 747, 770, 771
BmrFI CCNGG 1 cut(s) 743
BmsI GCATC 3 cut(s) 637, 703, 869
BpiI GAAGAC 2 cut(s) 98, 538
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpmI CTGGAG 2 cut(s) 162, 528
BpuEI CTTGAG 2 cut(s) 221, 222
BpuMI CCSGG 1 cut(s) 743
Bsa29I ATCGAT 1 cut(s) 1014
BsaBI GATNNNNATC 1 cut(s) 1077
BsaXI ACNNNNNCTCC 2 cut(s) 869, 899
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse118I RCCGGY 1 cut(s) 808
Bse1I ACTGG 1 cut(s) 540
Bse21I CCTNAGG 1 cut(s) 774
Bse3DI GCAATG 1 cut(s) 934
Bse8I GATNNNNATC 1 cut(s) 1077
BseCI ATCGAT 1 cut(s) 1014
BseGI GGATG 4 cut(s) 33, 628, 792, 832
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMI GCAATG 1 cut(s) 934
BseMII CTCAG 4 cut(s) 570, 656, 765, 1137
BseNI ACTGG 1 cut(s) 540
BseRI GAGGAG 3 cut(s) 626, 1035, 1103
BseXI GCAGC 2 cut(s) 271, 983
Bsh1236I CGCG 1 cut(s) 925
Bsh1285I CGRYCG 1 cut(s) 704
BshFI GGCC 1 cut(s) 563
BshVI ATCGAT 1 cut(s) 1014
BsiEI CGRYCG 1 cut(s) 704
BsiHKAI GWGCWC 1 cut(s) 882
BsiHKCI CYCGRG 1 cut(s) 516
BsiSI CCGG 3 cut(s) 412, 742, 809
BslFI GGGAC 1 cut(s) 755
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 2 cut(s) 75, 1024
BsmBI CGTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 755
BsnI GGCC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 882
Bsp143I GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
BspACI CCGC 4 cut(s) 25, 415, 923, 1027
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 4 cut(s) 571, 655, 766, 1136
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 1 cut(s) 925
BspHI TCATGA 1 cut(s) 1039
BspLI GGNNCC 3 cut(s) 747, 770, 771
BspPI GGATC 3 cut(s) 479, 632, 1008
BsrDI GCAATG 1 cut(s) 934
BsrFI RCCGGY 1 cut(s) 808
BsrI ACTGG 1 cut(s) 540
BssAI RCCGGY 1 cut(s) 808
BssMI GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
Bst4CI ACNGT 3 cut(s) 702, 820, 1054
Bst6I CTCTTC 2 cut(s) 314, 974
BstC8I GCNNGC 3 cut(s) 197, 810, 923
BstDEI CTNAG 6 cut(s) 579, 642, 774, 797, 962, 1123
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 4 cut(s) 33, 628, 792, 832
BstFNI CGCG 1 cut(s) 925
BstHHI GCGC 2 cut(s) 621, 927
BstKTI GATC 7 cut(s) 70, 190, 474, 640, 844, 1003, 1014
BstMAI GTCTC 2 cut(s) 75, 1024
BstMBI GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
BstMCI CGRYCG 1 cut(s) 704
BstMWI GCNNNNNNNGC 4 cut(s) 380, 421, 625, 809
BstNSI RCATGY 1 cut(s) 921
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 741
BstUI CGCG 1 cut(s) 925
BstV1I GCAGC 2 cut(s) 271, 983
BstV2I GAAGAC 2 cut(s) 98, 538
BstX2I RGATCY 1 cut(s) 1000
BstXI CCANNNNNNTGG 1 cut(s) 140
BstYI RGATCY 1 cut(s) 1000
Bsu15I ATCGAT 1 cut(s) 1014
Bsu36I CCTNAGG 1 cut(s) 774
BsuRI GGCC 1 cut(s) 563
BsuTUI ATCGAT 1 cut(s) 1014
BtsCI GGATG 4 cut(s) 33, 628, 792, 832
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 3 cut(s) 197, 810, 923
CciI TCATGA 1 cut(s) 1039
CfoI GCGC 2 cut(s) 621, 927
Cfr10I RCCGGY 1 cut(s) 808
Cfr13I GGNCC 5 cut(s) 209, 233, 275, 769, 1145
ClaI ATCGAT 1 cut(s) 1014
CseI GACGC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 821, 1095
CviAII CATG 6 cut(s) 39, 134, 269, 344, 918, 1040
CviQI GTAC 2 cut(s) 821, 1095
DdeI CTNAG 6 cut(s) 579, 642, 774, 797, 962, 1123
DpnI GATC 7 cut(s) 69, 189, 473, 639, 843, 1002, 1013
DpnII GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
DraI TTTAAA 1 cut(s) 550
Eam1104I CTCTTC 2 cut(s) 314, 974
EarI CTCTTC 2 cut(s) 314, 974
Eco47I GGWCC 5 cut(s) 209, 233, 275, 769, 1145
Eco57I CTGAAG 1 cut(s) 300
Eco81I CCTNAGG 1 cut(s) 774
Eco88I CYCGRG 1 cut(s) 516
Eco91I GGTNACC 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 769
EcoO65I GGTNACC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 734
EcoT22I ATGCAT 1 cut(s) 718
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 6 cut(s) 42, 137, 272, 347, 921, 1043
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 1 cut(s) 755
FatI CATG 6 cut(s) 38, 133, 268, 343, 917, 1039
FauI CCCGC 1 cut(s) 930
FauNDI CATATG 1 cut(s) 476
Fnu4HI GCNGC 3 cut(s) 285, 416, 972
FokI GGATG 4 cut(s) 40, 615, 779, 819
Fsp4HI GCNGC 3 cut(s) 285, 416, 972
FspBI CTAG 2 cut(s) 78, 527
GlaI GCGC 2 cut(s) 620, 926
GluI GCNGC 3 cut(s) 285, 416, 972
GsuI CTGGAG 2 cut(s) 162, 528
HaeIII GGCC 1 cut(s) 563
HapII CCGG 3 cut(s) 412, 742, 809
HgaI GACGC 1 cut(s) 92
HhaI GCGC 2 cut(s) 621, 927
Hin1II CATG 6 cut(s) 42, 137, 272, 347, 921, 1043
Hin6I GCGC 2 cut(s) 619, 925
HinP1I GCGC 2 cut(s) 619, 925
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 7 cut(s) 122, 203, 227, 340, 512, 599, 1073
HpaII CCGG 3 cut(s) 412, 742, 809
HphI GGTGA 2 cut(s) 722, 1145
Hpy166II GTNNAC 4 cut(s) 330, 823, 904, 913
Hpy188I TCNGA 5 cut(s) 337, 580, 645, 865, 1000
Hpy8I GTNNAC 4 cut(s) 330, 823, 904, 913
HpyAV CCTTC 2 cut(s) 63, 1158
HpyCH4III ACNGT 3 cut(s) 702, 820, 1054
HpyCH4V TGCA 6 cut(s) 500, 590, 628, 716, 860, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 380, 421, 625, 809
HpyF3I CTNAG 6 cut(s) 579, 642, 774, 797, 962, 1123
Hsp92II CATG 6 cut(s) 42, 137, 272, 347, 921, 1043
HspAI GCGC 2 cut(s) 619, 925
KflI GGGWCCC 1 cut(s) 769
KroI GCCGGC 1 cut(s) 808
KroNI GCCGGC 1 cut(s) 810
Kzo9I GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
LmnI GCTCC 2 cut(s) 452, 877
Lsp1109I GCAGC 2 cut(s) 271, 983
LweI GCATC 3 cut(s) 637, 703, 869
MaeI CTAG 2 cut(s) 78, 527
MaeIII GTNAC 1 cut(s) 128
MalI GATC 7 cut(s) 69, 189, 473, 639, 843, 1002, 1013
MboI GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
MboII GAAGA 9 cut(s) 98, 170, 197, 301, 401, 404, 543, 851, 991
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 1 cut(s) 882
MluCI AATT 3 cut(s) 552, 734, 813
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 2 cut(s) 23, 430
Mph1103I ATGCAT 1 cut(s) 718
MroNI GCCGGC 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 396
MseI TTAA 5 cut(s) 60, 297, 429, 549, 722
MslI CAYNNNNRTG 3 cut(s) 633, 1038, 1061
MspI CCGG 3 cut(s) 412, 742, 809
MspR9I CCNGG 1 cut(s) 743
MvnI CGCG 1 cut(s) 925
MwoI GCNNNNNNNGC 4 cut(s) 380, 421, 625, 809
NaeI GCCGGC 1 cut(s) 810
NciI CCSGG 1 cut(s) 743
NdeI CATATG 1 cut(s) 476
NdeII GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
NgoMIV GCCGGC 1 cut(s) 808
NlaIII CATG 6 cut(s) 42, 137, 272, 347, 921, 1043
NlaIV GGNNCC 3 cut(s) 747, 770, 771
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 921
PagI TCATGA 1 cut(s) 1039
PdiI GCCGGC 1 cut(s) 810
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 6 cut(s) 122, 227, 340, 512, 599, 1073
PkrI GCNGC 3 cut(s) 286, 417, 973
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 769
PsiI TTATAA 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 769
PspEI GGTNACC 1 cut(s) 128
PspN4I GGNNCC 3 cut(s) 747, 770, 771
PspPI GGNCC 5 cut(s) 209, 233, 275, 769, 1145
PspPPI RGGWCCY 1 cut(s) 769
PsuI RGATCY 1 cut(s) 1000
RsaI GTAC 2 cut(s) 822, 1096
RsaNI GTAC 2 cut(s) 821, 1095
RseI CAYNNNNRTG 3 cut(s) 633, 1038, 1061
SaqAI TTAA 5 cut(s) 60, 297, 429, 549, 722
SatI GCNGC 3 cut(s) 285, 416, 972
Sau3AI GATC 7 cut(s) 67, 187, 471, 637, 841, 1000, 1011
Sau96I GGNCC 5 cut(s) 209, 233, 275, 769, 1145
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 743
SduI GDGCHC 1 cut(s) 882
SfaNI GCATC 3 cut(s) 637, 703, 869
SinI GGWCC 5 cut(s) 209, 233, 275, 769, 1145
SmiMI CAYNNNNRTG 3 cut(s) 633, 1038, 1061
SmlI CTYRAG 2 cut(s) 200, 237
SmoI CTYRAG 2 cut(s) 200, 237
Sse9I AATT 3 cut(s) 552, 734, 813
SsiI CCGC 4 cut(s) 25, 415, 923, 1027
SspMI CTAG 2 cut(s) 78, 527
StyD4I CCNGG 1 cut(s) 741
TaaI ACNGT 3 cut(s) 702, 820, 1054
TaqI TCGA 5 cut(s) 94, 183, 312, 704, 1014
TasI AATT 3 cut(s) 552, 734, 813
TatI WGTACW 1 cut(s) 820
TauI GCSGC 1 cut(s) 418
TfiI GAWTC 6 cut(s) 122, 227, 340, 512, 599, 1073
Tru1I TTAA 5 cut(s) 60, 297, 429, 549, 722
Tru9I TTAA 5 cut(s) 60, 297, 429, 549, 722
TscAI CASTG 1 cut(s) 425
TseI GCWGC 2 cut(s) 284, 971
TspDTI ATGAA 1 cut(s) 1028
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 1 cut(s) 425
VpaK11BI GGWCC 5 cut(s) 209, 233, 275, 769, 1145
XapI RAATTY 1 cut(s) 734
XceI RCATGY 1 cut(s) 921
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 2 cut(s) 78, 527
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.