AT3G55050

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005979
Physical Location & Seq
Reverse (-)
20400430 .. 20402595
2166 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55050.1

Sequence Viewer

Length: 1155 bp
ATGGTATCTACAACATTTAGGAGAATTGTATCGCCTTGTTGGAGACCTTTTGGTATTGGAGAAGATTCTAGTCCTGGTAGTGATGATACGAATGGCCGTCTCGATGGTCTCTTGTGGTACAAAGATTCTGGGAACCACATCACTGGAGAGTTTTCTATGGCAGTCGTTCAAGCTAACAACCTTCTTGAAGATCATAGCCAGTTAGAATCTGGTCCTATTAGTTTGCATGAGTCTGGACCTGAGGCAACTTTTGTTGGTGTTTATGATGGTCATGGAGGTCCCGAGGCTGCTCGGTTTGTAAACGATAGGCTCTTTTATAACATCAAGAGGTACACATCAGAACAGCGAGGGATGTCGCCTGATGTTATCACCAGAGGGTTTGTAGCAACAGAGGAGGAGTTTCTCGGTTTAGTGCAGGAGCAATGGAAGACCAAGCCACAGATTGCTTCTGTGGGAGCTTGTTGTTTGGTTGGTATTGTTTGTAATGGATTGTTGTATGTTGCAAACGCAGGAGATTCTCGGGTTGTGTTAGGGAAAGTCGCAAATCCGTTTAAAGAGTTGAAAGCTGTTCAGTTATCAACAGAGCATAATGCAAGTATCGAATCTGTGAGAGAGGAGTTACGTTTGTTGCATCCTGATGATCCGAATATCGTGGTCTTGAAACACAAAGTTTGGCGTGTTAAAGGGATTATACAGGTCTCAAGATCCATTGGTGACGCATACCTTAAAAGAGCGGAGTTTAATCAAGAACCGCTACTACCTAAGTTTAGGGTTCCAGAACGTTTTGAAAAGCCGATCATGAGAGCGGAACCGACTATAACAGTTCATAAGATTCATCCGGAAGATCAGTTTCTTATATTTGCCTCGGATGGTTTATGGGAGCATCTAAGCAACCAGGAAGCTGTTGATATTGTCAATTCTTGTCCACGCAATGGTGTGGCTCGGAAGTTAGTGAAAGCTGCATTACAAGAAGCAGCAAAGAAGAGAGAGATGAGGTATTCTGATTTGGAGAAGATAGAACGTGGCATTCGGCGACACTTTCACGACGATATTACCGTTATTGTTGTCTTCCTCCACGCTACAAACTTCGCTACTCGAACTCCGATCTCTGTCAAAGGAGGTGGTCTCCTCTCAGCCCATAATCCCGTTTTATAG

Protein Analysis

384

Amino Acids

42.85

Weight (kDa)

6.71

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 77 - 327 1.2e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
AccB7I CCANNNNNTGG 1 cut(s) 932
AccBSI CCGCTC 2 cut(s) 734, 806
AccIII TCCGGA 1 cut(s) 838
AciI CCGC 3 cut(s) 734, 752, 806
AclI AACGTT 1 cut(s) 781
AclWI GGATC 2 cut(s) 635, 699
AcoI YGGCCR 1 cut(s) 94
AfaI GTAC 2 cut(s) 119, 332
AfiI CCNNNNNNNGG 1 cut(s) 932
AgsI TTSAA 5 cut(s) 170, 188, 562, 661, 788
AjnI CCWGG 2 cut(s) 73, 894
AluBI AGCT 5 cut(s) 173, 458, 566, 902, 959
AluI AGCT 5 cut(s) 173, 458, 566, 902, 959
Alw26I GTCTC 5 cut(s) 37, 104, 113, 703, 1130
AlwI GGATC 2 cut(s) 635, 699
Ama87I CYCGRG 2 cut(s) 281, 519
Aor13HI TCCGGA 1 cut(s) 838
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 3 cut(s) 287, 959, 974
AspS9I GGNCC 3 cut(s) 212, 236, 278
AsuHPI GGTGA 2 cut(s) 361, 725
AvaI CYCGRG 2 cut(s) 281, 519
AvaII GGWCC 3 cut(s) 212, 236, 278
AxyI CCTNAGG 1 cut(s) 240
BbsI GAAGAC 2 cut(s) 434, 1060
BbvI GCAGC 3 cut(s) 274, 946, 986
BccI CCATC 3 cut(s) 98, 260, 863
BceAI ACGGC 1 cut(s) 81
BciT130I CCWGG 2 cut(s) 75, 896
BcoDI GTCTC 5 cut(s) 37, 104, 113, 703, 1130
BfaI CTAG 1 cut(s) 69
BisI GCNGC 3 cut(s) 288, 960, 975
BlsI GCNGC 3 cut(s) 289, 961, 976
Bme1390I CCNGG 2 cut(s) 75, 896
Bme18I GGWCC 3 cut(s) 212, 236, 278
BmeT110I CYCGRG 2 cut(s) 281, 519
BmgT120I GGNCC 3 cut(s) 212, 236, 278
BmiI GGNNCC 4 cut(s) 134, 280, 774, 810
BmrFI CCNGG 2 cut(s) 75, 896
BmsI GCATC 2 cut(s) 640, 892
BpiI GAAGAC 2 cut(s) 434, 1060
BplI GAGNNNNNCTC 2 cut(s) 1110, 1142
BpmI CTGGAG 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 685
BsaI GGTCTC 4 cut(s) 37, 113, 703, 1130
BsaJI CCNNGG 2 cut(s) 282, 864
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 2 cut(s) 1084, 1114
Bsc4I CCNNNNNNNGG 1 cut(s) 932
Bse1I ACTGG 2 cut(s) 148, 199
Bse21I CCTNAGG 1 cut(s) 240
Bse3DI GCAATG 2 cut(s) 428, 937
BseAI TCCGGA 1 cut(s) 838
BseBI CCWGG 2 cut(s) 75, 896
BseDI CCNNGG 2 cut(s) 282, 864
BseGI GGATG 4 cut(s) 357, 631, 835, 874
BseLI CCNNNNNNNGG 1 cut(s) 932
BseMI GCAATG 2 cut(s) 428, 937
BseMII CTCAG 2 cut(s) 231, 1146
BseNI ACTGG 2 cut(s) 148, 199
BseRI GAGGAG 4 cut(s) 407, 410, 629, 1118
BseXI GCAGC 3 cut(s) 274, 946, 986
BsgI GTGCAG 1 cut(s) 434
BshFI GGCC 1 cut(s) 96
BsiHKCI CYCGRG 2 cut(s) 281, 519
BsiSI CCGG 1 cut(s) 839
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 1 cut(s) 932
BsmAI GTCTC 5 cut(s) 37, 104, 113, 703, 1130
BsmBI CGTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 264
BsmI GAATGC 1 cut(s) 1026
BsnI GGCC 1 cut(s) 96
Bso31I GGTCTC 4 cut(s) 37, 113, 703, 1130
BsoBI CYCGRG 2 cut(s) 281, 519
Bsp13I TCCGGA 1 cut(s) 838
Bsp143I GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
BspACI CCGC 3 cut(s) 734, 752, 806
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 2 cut(s) 232, 1145
BspEI TCCGGA 1 cut(s) 838
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 4 cut(s) 134, 280, 774, 810
BspPI GGATC 2 cut(s) 635, 699
BspTNI GGTCTC 4 cut(s) 37, 113, 703, 1130
BsrBI CCGCTC 2 cut(s) 734, 806
BsrDI GCAATG 2 cut(s) 428, 937
BsrI ACTGG 2 cut(s) 148, 199
BssECI CCNNGG 2 cut(s) 282, 864
BssMI GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
Bst2UI CCWGG 2 cut(s) 75, 896
Bst4CI ACNGT 2 cut(s) 823, 1057
Bst6I CTCTTC 1 cut(s) 977
BstDEI CTNAG 4 cut(s) 240, 762, 887, 1132
BstF5I GGATG 4 cut(s) 357, 631, 835, 874
BstKTI GATC 6 cut(s) 193, 643, 707, 798, 847, 1107
BstMAI GTCTC 5 cut(s) 37, 104, 113, 703, 1130
BstMBI GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
BstNI CCWGG 2 cut(s) 75, 896
BstSCI CCNGG 2 cut(s) 73, 894
BstV1I GCAGC 3 cut(s) 274, 946, 986
BstV2I GAAGAC 2 cut(s) 434, 1060
BstX2I RGATCY 1 cut(s) 704
BstXI CCANNNNNNTGG 1 cut(s) 143
BstYI RGATCY 1 cut(s) 704
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 1 cut(s) 96
BtsCI GGATG 4 cut(s) 357, 631, 835, 874
BtsIMutI CAGTG 1 cut(s) 141
CciI TCATGA 1 cut(s) 798
Cfr13I GGNCC 3 cut(s) 212, 236, 278
CseI GACGC 1 cut(s) 725
Csp6I GTAC 2 cut(s) 118, 331
CspCI CAANNNNNGTGG 2 cut(s) 1102, 1137
CviAII CATG 3 cut(s) 227, 272, 799
CviQI GTAC 2 cut(s) 118, 331
DdeI CTNAG 4 cut(s) 240, 762, 887, 1132
DpnI GATC 6 cut(s) 192, 642, 706, 797, 846, 1106
DpnII GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
DraI TTTAAA 1 cut(s) 553
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 977
EarI CTCTTC 1 cut(s) 977
Eco31I GGTCTC 4 cut(s) 37, 113, 703, 1130
Eco47I GGWCC 3 cut(s) 212, 236, 278
Eco81I CCTNAGG 1 cut(s) 240
Eco88I CYCGRG 2 cut(s) 281, 519
EcoO109I RGGNCCY 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 73, 894
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 3 cut(s) 230, 275, 802
FaqI GGGAC 1 cut(s) 264
FatI CATG 3 cut(s) 226, 271, 798
Fnu4HI GCNGC 3 cut(s) 288, 960, 975
FokI GGATG 4 cut(s) 364, 618, 822, 881
Fsp4HI GCNGC 3 cut(s) 288, 960, 975
FspBI CTAG 1 cut(s) 69
GluI GCNGC 3 cut(s) 288, 960, 975
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 1 cut(s) 96
HapII CCGG 1 cut(s) 839
HgaI GACGC 1 cut(s) 725
Hin1II CATG 3 cut(s) 230, 275, 802
HinfI GANTC 7 cut(s) 65, 125, 206, 230, 515, 602, 832
HpaII CCGG 1 cut(s) 839
HphI GGTGA 2 cut(s) 361, 725
Hpy166II GTNNAC 3 cut(s) 301, 333, 926
Hpy188I TCNGA 6 cut(s) 340, 645, 868, 945, 1003, 1104
Hpy8I GTNNAC 3 cut(s) 301, 333, 926
Hpy99I CGWCG 1 cut(s) 1049
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 2 cut(s) 823, 1057
HpyCH4IV ACGT 3 cut(s) 622, 781, 1021
HpyCH4V TGCA 6 cut(s) 226, 415, 503, 593, 631, 962
HpyF3I CTNAG 4 cut(s) 240, 762, 887, 1132
HpySE526I ACGT 3 cut(s) 622, 781, 1021
Hsp92II CATG 3 cut(s) 230, 275, 802
Kpn2I TCCGGA 1 cut(s) 838
Kzo9I GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
LmnI GCTCC 3 cut(s) 418, 455, 880
Lsp1109I GCAGC 3 cut(s) 274, 946, 986
LweI GCATC 2 cut(s) 640, 892
MaeI CTAG 1 cut(s) 69
MaeII ACGT 3 cut(s) 622, 781, 1021
MaeIII GTNAC 2 cut(s) 618, 713
MalI GATC 6 cut(s) 192, 642, 706, 797, 846, 1106
MbiI CCGCTC 2 cut(s) 734, 806
MboI GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
MboII GAAGA 7 cut(s) 74, 200, 439, 854, 994, 1024, 1060
MflI RGATCY 1 cut(s) 704
MluCI AATT 2 cut(s) 24, 916
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 20
MroI TCCGGA 1 cut(s) 838
MseI TTAA 4 cut(s) 552, 681, 726, 741
MslI CAYNNNNRTG 1 cut(s) 636
MspI CCGG 1 cut(s) 839
MspR9I CCNGG 2 cut(s) 75, 896
Mva1269I GAATGC 1 cut(s) 1026
MvaI CCWGG 2 cut(s) 75, 896
NdeII GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
NlaIII CATG 3 cut(s) 230, 275, 802
NlaIV GGNNCC 4 cut(s) 134, 280, 774, 810
NmuCI GTSAC 1 cut(s) 713
PagI TCATGA 1 cut(s) 798
PcsI WCGNNNNNNNCGW 1 cut(s) 1053
PctI GAATGC 1 cut(s) 1026
PfeI GAWTC 6 cut(s) 65, 125, 206, 515, 602, 832
PflMI CCANNNNNTGG 1 cut(s) 932
PkrI GCNGC 3 cut(s) 289, 961, 976
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PpuMI RGGWCCY 1 cut(s) 278
PsiI TTATAA 1 cut(s) 318
Psp1406I AACGTT 1 cut(s) 781
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 73, 894
PspGI CCWGG 2 cut(s) 73, 894
PspN4I GGNNCC 4 cut(s) 134, 280, 774, 810
PspPI GGNCC 3 cut(s) 212, 236, 278
PspPPI RGGWCCY 1 cut(s) 278
PsrI GAACNNNNNNTAC 2 cut(s) 741, 773
PsuI RGATCY 1 cut(s) 704
RsaI GTAC 2 cut(s) 119, 332
RsaNI GTAC 2 cut(s) 118, 331
RseI CAYNNNNRTG 1 cut(s) 636
SaqAI TTAA 4 cut(s) 552, 681, 726, 741
SatI GCNGC 3 cut(s) 288, 960, 975
Sau3AI GATC 6 cut(s) 190, 640, 704, 795, 844, 1104
Sau96I GGNCC 3 cut(s) 212, 236, 278
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 75, 896
SfaNI GCATC 2 cut(s) 640, 892
SinI GGWCC 3 cut(s) 212, 236, 278
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
Sse9I AATT 2 cut(s) 24, 916
SsiI CCGC 3 cut(s) 734, 752, 806
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 73, 894
TaaI ACNGT 2 cut(s) 823, 1057
TaiI ACGT 3 cut(s) 625, 784, 1024
TaqI TCGA 3 cut(s) 102, 600, 1096
TasI AATT 2 cut(s) 24, 916
TfiI GAWTC 6 cut(s) 65, 125, 206, 515, 602, 832
Tru1I TTAA 4 cut(s) 552, 681, 726, 741
Tru9I TTAA 4 cut(s) 552, 681, 726, 741
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 3 cut(s) 287, 959, 974
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 2 cut(s) 815, 824
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 932
VpaK11BI GGWCC 3 cut(s) 212, 236, 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 206
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.