Rroxscaffold_4G00292180

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005979
Physical Location & Seq
Forward (+)
12548635 .. 12552656
4022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292180.1

Sequence Viewer

Length: 903 bp
ATGGTATCAGCTACACTAATGAAGATTATGGCACCGTGTTGGAGACCTTCTGTTGAAGGTGAAAATTCTAATAGAGCTGGGGATGCGAGTGGTCGAGTTAATGGTTTATTGTGGTATAAAGATTTGGGAAACCATGTTAATGGTGAATTCTCGATGGCAGTAATTCAAGCAAACAATTTGTTGGAAGACCATAGCCAACTTGAATCCGGACCGTTGAGCTTGCTCGAATCAGGTCCCCATGGAACATTTGTTGGAGTTTATGATGGACATGGAGGTCCAGAAGCAGCCCGATTTGTAAATGATCACCTGTTCAAGAATCTCAAGAGCATAGTATGTCGTGGACTGCTATACATAGCAAATGCGGGAGATTCTCGGGCGGTGTTAGGAAGACTGGAAAAGACCGTCAAACAGGTAAAAGCTGTTCAGTTATCAGTTGAGCACAATGCAAGTATAGAATCTGTGAGAGAGGAACTGCACTCGTTGCATCCCGATGATCCTCAGATTGTGGTAATGAAGCACAAGGTGTGGCGCGTGAAGGGTCTGATACAGATTTCAAGGTCCATTGGTGATGCCTACCTGAAAAGGTCGGAATTTAACAAAGAACCTCTGTTGGCAAAGTTTAGACTATCAGAACCCTTCCACAAGCCGATCCTTAAAGCTGAGCCAACAATATTGGTGCAAAAACTCTACCCTGAAGATCAGTTTCTCATATTTGCATCGGATGGCCTATGGGAGCAGCTGAGCAATCAGGAGGCAGTTGACATTGTTCAGAACTATCCACGTAATGGAATTGCAAGGAGACTTGTCAAAGCTGCACTTCGTGAAGCAGCAAAGAAGAGAGAAATGAGATACTCAGACTTTGAAAAAGATCGACCGAGGGGTGAGGAGACATTTTCACGATGA

Protein Analysis

300

Amino Acids

33.75

Weight (kDa)

8.72

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 116 - 278 3.1e-27 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 273
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 785
AccII CGCG 1 cut(s) 531
AccIII TCCGGA 1 cut(s) 206
AciI CCGC 2 cut(s) 362, 377
AclWI GGATC 2 cut(s) 488, 643
AcsI RAATTY 3 cut(s) 64, 146, 590
AcuI CTGAAG 1 cut(s) 714
AdeI CACNNNGTG 2 cut(s) 523, 821
AfiI CCNNNNNNNGG 1 cut(s) 785
AgsI TTSAA 6 cut(s) 56, 167, 203, 313, 555, 863
AluBI AGCT 7 cut(s) 11, 77, 219, 419, 659, 739, 812
AluI AGCT 7 cut(s) 11, 77, 219, 419, 659, 739, 812
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 3 cut(s) 37, 793, 881
AlwI GGATC 2 cut(s) 488, 643
Ama87I CYCGRG 1 cut(s) 372
Aor13HI TCCGGA 1 cut(s) 206
AoxI GGCC 1 cut(s) 724
ApeKI GCWGC 4 cut(s) 284, 736, 812, 827
ApoI RAATTY 3 cut(s) 64, 146, 590
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 4 cut(s) 209, 233, 275, 558
AsuHPI GGTGA 5 cut(s) 71, 155, 296, 578, 893
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 4 cut(s) 209, 233, 275, 558
BanI GGYRCC 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 192, 394
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 4 cut(s) 296, 748, 799, 839
BccI CCATC 3 cut(s) 148, 257, 716
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 3 cut(s) 37, 793, 881
BisI GCNGC 4 cut(s) 285, 737, 813, 828
BlpI GCTNAGC 2 cut(s) 660, 740
BlsI GCNGC 4 cut(s) 286, 738, 814, 829
Bme18I GGWCC 4 cut(s) 209, 233, 275, 558
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 4 cut(s) 209, 233, 275, 558
BmiI GGNNCC 2 cut(s) 33, 235
BmsI GCATC 4 cut(s) 73, 493, 559, 725
BpiI GAAGAC 2 cut(s) 192, 394
Bpu1102I GCTNAGC 2 cut(s) 660, 740
BpuEI CTTGAG 1 cut(s) 305
BsaAI YACGTR 1 cut(s) 782
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 238, 875
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 785
Bse1I ACTGG 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 206
BseDI CCNNGG 2 cut(s) 238, 875
BseGI GGATG 3 cut(s) 88, 484, 727
BseLI CCNNNNNNNGG 1 cut(s) 785
BseMII CTCAG 4 cut(s) 512, 651, 731, 867
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 899
BseXI GCAGC 4 cut(s) 296, 748, 799, 839
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 2 cut(s) 458, 798
Bsh1236I CGCG 1 cut(s) 531
Bsh1285I CGRYCG 1 cut(s) 875
BshFI GGCC 1 cut(s) 726
BshNI GGYRCC 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 875
BsiHKAI GWGCWC 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 372
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 785
BsmAI GTCTC 3 cut(s) 37, 793, 881
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 1 cut(s) 726
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 441
Bsp13I TCCGGA 1 cut(s) 206
Bsp143I GATC 5 cut(s) 301, 493, 648, 697, 868
Bsp1720I GCTNAGC 2 cut(s) 660, 740
Bsp19I CCATGG 1 cut(s) 238
BspACI CCGC 2 cut(s) 362, 377
BspANI GGCC 1 cut(s) 726
BspCNI CTCAG 4 cut(s) 511, 652, 732, 866
BspEI TCCGGA 1 cut(s) 206
BspFNI CGCG 1 cut(s) 531
BspLI GGNNCC 2 cut(s) 33, 235
BspPI GGATC 2 cut(s) 488, 643
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 37
BsrI ACTGG 1 cut(s) 396
BssECI CCNNGG 2 cut(s) 238, 875
BssMI GATC 5 cut(s) 301, 493, 648, 697, 868
BssT1I CCWWGG 1 cut(s) 238
Bst4CI ACNGT 3 cut(s) 36, 213, 403
Bst6I CTCTTC 1 cut(s) 830
BstAPI GCANNNNNTGC 1 cut(s) 481
BstBAI YACGTR 1 cut(s) 782
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 4 cut(s) 498, 660, 740, 853
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 3 cut(s) 88, 484, 727
BstFNI CGCG 1 cut(s) 531
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 5 cut(s) 304, 496, 651, 700, 871
BstMAI GTCTC 3 cut(s) 37, 793, 881
BstMBI GATC 5 cut(s) 301, 493, 648, 697, 868
BstMCI CGRYCG 1 cut(s) 875
BstMWI GCNNNNNNNGC 2 cut(s) 83, 481
BstUI CGCG 1 cut(s) 531
BstV1I GCAGC 4 cut(s) 296, 748, 799, 839
BstV2I GAAGAC 2 cut(s) 192, 394
BstXI CCANNNNNNTGG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 726
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 88, 484, 727
Cac8I GCNNGC 1 cut(s) 221
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 4 cut(s) 209, 233, 275, 558
CpoI CGGWCCG 1 cut(s) 209
CspI CGGWCCG 1 cut(s) 209
CviAII CATG 3 cut(s) 134, 239, 269
DdeI CTNAG 4 cut(s) 498, 660, 740, 853
DpnI GATC 5 cut(s) 303, 495, 650, 699, 870
DpnII GATC 5 cut(s) 301, 493, 648, 697, 868
DraIII CACNNNGTG 2 cut(s) 523, 821
DrdI GACNNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 830
EarI CTCTTC 1 cut(s) 830
Eco130I CCWWGG 1 cut(s) 238
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 4 cut(s) 209, 233, 275, 558
Eco57I CTGAAG 1 cut(s) 714
Eco88I CYCGRG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 3 cut(s) 137, 242, 272
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 1 cut(s) 219
FatI CATG 3 cut(s) 133, 238, 268
FauI CCCGC 1 cut(s) 355
FbaI TGATCA 1 cut(s) 301
Fnu4HI GCNGC 4 cut(s) 285, 737, 813, 828
FokI GGATG 3 cut(s) 95, 471, 734
Fsp4HI GCNGC 4 cut(s) 285, 737, 813, 828
GlaI GCGC 1 cut(s) 530
GluI GCNGC 4 cut(s) 285, 737, 813, 828
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 1 cut(s) 726
HapII CCGG 1 cut(s) 207
HhaI GCGC 1 cut(s) 531
Hin1II CATG 3 cut(s) 137, 242, 272
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HinfI GANTC 5 cut(s) 203, 227, 316, 368, 455
HpaII CCGG 1 cut(s) 207
HphI GGTGA 5 cut(s) 71, 155, 296, 578, 893
Hpy166II GTNNAC 2 cut(s) 341, 760
Hpy188I TCNGA 7 cut(s) 501, 543, 589, 631, 721, 771, 856
Hpy188III TCNNGA 9 cut(s) 151, 207, 278, 313, 322, 488, 749, 821, 897
Hpy8I GTNNAC 2 cut(s) 341, 760
HpyAV CCTTC 4 cut(s) 50, 57, 529, 646
HpyCH4III ACNGT 3 cut(s) 36, 213, 403
HpyCH4IV ACGT 1 cut(s) 781
HpyCH4V TGCA 7 cut(s) 446, 475, 484, 679, 716, 794, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 481
HpyF3I CTNAG 4 cut(s) 498, 660, 740, 853
HpySE526I ACGT 1 cut(s) 781
Hsp92II CATG 3 cut(s) 137, 242, 272
HspAI GCGC 1 cut(s) 529
Kpn2I TCCGGA 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 5 cut(s) 301, 493, 648, 697, 868
LmnI GCTCC 1 cut(s) 733
Lsp1109I GCAGC 4 cut(s) 296, 748, 799, 839
LweI GCATC 4 cut(s) 73, 493, 559, 725
MaeII ACGT 1 cut(s) 781
MalI GATC 5 cut(s) 303, 495, 650, 699, 870
MboI GATC 5 cut(s) 301, 493, 648, 697, 868
MboII GAAGA 5 cut(s) 34, 197, 399, 707, 847
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 6 cut(s) 64, 146, 162, 175, 590, 789
MmeI TCCRAC 4 cut(s) 20, 162, 232, 567
MnlI CCTC 7 cut(s) 266, 460, 507, 615, 745, 870, 877
MroI TCCGGA 1 cut(s) 206
MseI TTAA 4 cut(s) 99, 138, 594, 654
MslI CAYNNNNRTG 2 cut(s) 138, 489
MspA1I CMGCKG 1 cut(s) 739
MspI CCGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 83, 481
NcoI CCATGG 1 cut(s) 238
NdeII GATC 5 cut(s) 301, 493, 648, 697, 868
NlaIII CATG 3 cut(s) 137, 242, 272
NlaIV GGNNCC 2 cut(s) 33, 235
PfeI GAWTC 5 cut(s) 203, 227, 316, 368, 455
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 4 cut(s) 286, 738, 814, 829
Ppu21I YACGTR 1 cut(s) 782
PpuMI RGGWCCY 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 233
PspFI CCCAGC 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 33, 235
PspPI GGNCC 4 cut(s) 209, 233, 275, 558
PspPPI RGGWCCY 1 cut(s) 233
PsrI GAACNNNNNNTAC 2 cut(s) 405, 437
PvuII CAGCTG 1 cut(s) 739
RseI CAYNNNNRTG 2 cut(s) 138, 489
Rsr2I CGGWCCG 1 cut(s) 209
RsrII CGGWCCG 1 cut(s) 209
SaqAI TTAA 4 cut(s) 99, 138, 594, 654
SatI GCNGC 4 cut(s) 285, 737, 813, 828
Sau3AI GATC 5 cut(s) 301, 493, 648, 697, 868
Sau96I GGNCC 4 cut(s) 209, 233, 275, 558
SduI GDGCHC 1 cut(s) 441
SfaNI GCATC 4 cut(s) 73, 493, 559, 725
SinI GGWCC 4 cut(s) 209, 233, 275, 558
SmiMI CAYNNNNRTG 2 cut(s) 138, 489
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 6 cut(s) 64, 146, 162, 175, 590, 789
SsiI CCGC 2 cut(s) 362, 377
SspI AATATT 1 cut(s) 672
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 3 cut(s) 36, 213, 403
TaiI ACGT 1 cut(s) 784
TaqI TCGA 4 cut(s) 94, 152, 225, 871
TaqII GACCGA 1 cut(s) 889
TasI AATT 6 cut(s) 64, 146, 162, 175, 590, 789
TfiI GAWTC 5 cut(s) 203, 227, 316, 368, 455
Tru1I TTAA 4 cut(s) 99, 138, 594, 654
Tru9I TTAA 4 cut(s) 99, 138, 594, 654
TseI GCWGC 4 cut(s) 284, 736, 812, 827
TspDTI ATGAA 2 cut(s) 35, 527
Van91I CCANNNNNTGG 1 cut(s) 785
VpaK11BI GGWCC 4 cut(s) 209, 233, 275, 558
XapI RAATTY 3 cut(s) 64, 146, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.