Rw1G028930

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005979
Physical Location & Seq
Reverse (-)
56624958 .. 56627766
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028930.1

Sequence Viewer

Length: 1155 bp
ATGGTATCAGCTACACTAATGAAGATTATGGCACCGTGTTGGAGACCTTCTGTTGAAGGTGAAAATTCTAATAGAGCTGGGGATGCAAGTGGTCGAGTTAATGGTTTATTGTGGTATAAAGATTTGGGAAACCATGTTAATGGTGAATTCTCGATGGCAGTAATTCAAGCAAACAATTTGTTGGAAGACCATAGCCAACTTGAATCCGGACCGTTGAGCTTGCTCGAATCAGGTCCCCATGGAACATTTGTTGGAGTTTATGATGGACATGGCGGTCCAGAAGCAGCCCGATTTGTAAATGATCACCTGTTCAAGAATCTCAAGAAATTCACATCAGAGATTCAGGGAATGTCAGCTGATGTTATCACCAAAGCATTCTTGGCAACCGAAGAGGAATTTCTATCTTTAGTAAGGAAGCAGTGGCTGATTAAGCCACTGCTTGCTTCAGTTGGTGCATGTTGTTTGGTAGGCATAGTATGTCGTGGACTGCTATACATAGCAAATGCGGGAGATTCTCGGGCGGTGTTAGGAAGACTGGAAAAGACAGTCAAACAGGTAAAAGCTGTTCAGTTATCAGTTGAGCACAATGCAAGTATAGAATCTGTGAGAGAGGAACTGCACTCGTTGCATCCCGATGATCCTCAGATTGTGGTAATGAAGCACAAGGTGTGGCGCGTGAAGGGTCTGATACAGATTTCAAGGTCCATTGGTGATGCCTACCTGAAAAGGTCGGAATTTAACAAAGAACCTCTGTTGGCAAAGTTTAGACTATCAGAACCCTTCCACAAGCCGATCCTTAAAGCTGAGCCAACAATATTGGTGCAAAAACTCTACCCTGAAGATCAGTTTCTCATATTTGCATCGGATGGCCTATGGGAGCAGCTGAGCAATCAGGAGGCAGTTGACATTGTTCAGAACTATCCACGTCATGGAATTGCAAGGAGACTTGTCAAAGCTGCACTTTGTGAAGCAGCAAAGAAGAGAGAAATGAGATACTCAGACTTGAAAAAGATCGACCGAGGGGTGAGGAGACATTTTCACGACGATATTACCGTAATAGTTCTGTTCCTAGATACACATCTGGTCAGTCGTAGCCACTCACACGGGCATCTGCTTTCAATTAAGGGAGGTAGCGGCGTCCCCGCCAACACCTAG

Protein Analysis

384

Amino Acids

42.74

Weight (kDa)

8.82

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 77 - 323 2.4e-35 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 273
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 929
AccII CGCG 1 cut(s) 675
AccIII TCCGGA 1 cut(s) 206
AciI CCGC 5 cut(s) 273, 506, 521, 1134, 1143
AclWI GGATC 2 cut(s) 632, 787
AcsI RAATTY 5 cut(s) 64, 146, 326, 395, 734
AcuI CTGAAG 2 cut(s) 429, 858
AcyI GRCGYC 1 cut(s) 1137
AdeI CACNNNGTG 2 cut(s) 667, 965
AfiI CCNNNNNNNGG 1 cut(s) 929
AgsI TTSAA 7 cut(s) 56, 167, 203, 313, 699, 1006, 1119
AjiI CACGTC 1 cut(s) 926
AluBI AGCT 8 cut(s) 11, 77, 219, 356, 563, 803, 883, 956
AluI AGCT 8 cut(s) 11, 77, 219, 356, 563, 803, 883, 956
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 3 cut(s) 37, 937, 1024
AlwI GGATC 2 cut(s) 632, 787
AlwNI CAGNNNCTG 1 cut(s) 424
Ama87I CYCGRG 1 cut(s) 516
Aor13HI TCCGGA 1 cut(s) 206
AoxI GGCC 1 cut(s) 868
ApeKI GCWGC 4 cut(s) 284, 880, 956, 971
ApoI RAATTY 5 cut(s) 64, 146, 326, 395, 734
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 4 cut(s) 209, 233, 275, 702
AsuHPI GGTGA 6 cut(s) 71, 155, 296, 358, 722, 1036
AvaI CYCGRG 1 cut(s) 516
AvaII GGWCC 4 cut(s) 209, 233, 275, 702
BanI GGYRCC 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 192, 538
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 4 cut(s) 296, 892, 943, 983
BccI CCATC 3 cut(s) 148, 257, 860
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 3 cut(s) 37, 937, 1024
BfaI CTAG 2 cut(s) 1070, 1153
BisI GCNGC 5 cut(s) 285, 881, 957, 972, 1135
BlpI GCTNAGC 2 cut(s) 804, 884
BlsI GCNGC 5 cut(s) 286, 882, 958, 973, 1136
Bme18I GGWCC 4 cut(s) 209, 233, 275, 702
BmeT110I CYCGRG 1 cut(s) 516
BmgBI CACGTC 1 cut(s) 926
BmgT120I GGNCC 4 cut(s) 209, 233, 275, 702
BmiI GGNNCC 2 cut(s) 33, 235
BmsI GCATC 5 cut(s) 73, 637, 703, 869, 1117
BpiI GAAGAC 2 cut(s) 192, 538
Bpu1102I GCTNAGC 2 cut(s) 804, 884
BpuEI CTTGAG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 1077
BsaHI GRCGYC 1 cut(s) 1137
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 238, 1018
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse1I ACTGG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 1077
BseAI TCCGGA 1 cut(s) 206
BseDI CCNNGG 2 cut(s) 238, 1018
BseGI GGATG 3 cut(s) 88, 628, 871
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMII CTCAG 4 cut(s) 656, 795, 875, 1011
BseNI ACTGG 1 cut(s) 540
BseRI GAGGAG 1 cut(s) 1042
BseXI GCAGC 4 cut(s) 296, 892, 943, 983
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 2 cut(s) 602, 942
Bsh1236I CGCG 1 cut(s) 675
Bsh1285I CGRYCG 1 cut(s) 1018
BshFI GGCC 1 cut(s) 870
BshNI GGYRCC 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 585
BsiHKCI CYCGRG 1 cut(s) 516
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 219, 1124
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 3 cut(s) 37, 937, 1024
BsmFI GGGAC 2 cut(s) 219, 1124
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 1 cut(s) 870
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 585
Bsp13I TCCGGA 1 cut(s) 206
Bsp143I GATC 5 cut(s) 301, 637, 792, 841, 1011
Bsp1720I GCTNAGC 2 cut(s) 804, 884
Bsp19I CCATGG 1 cut(s) 238
BspACI CCGC 5 cut(s) 273, 506, 521, 1134, 1143
BspANI GGCC 1 cut(s) 870
BspCNI CTCAG 4 cut(s) 655, 796, 876, 1010
BspEI TCCGGA 1 cut(s) 206
BspFNI CGCG 1 cut(s) 675
BspLI GGNNCC 2 cut(s) 33, 235
BspPI GGATC 2 cut(s) 632, 787
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 37
BsrI ACTGG 1 cut(s) 540
BssECI CCNNGG 2 cut(s) 238, 1018
BssMI GATC 5 cut(s) 301, 637, 792, 841, 1011
BssNI GRCGYC 1 cut(s) 1137
BssT1I CCWWGG 1 cut(s) 238
Bst4CI ACNGT 4 cut(s) 36, 213, 547, 1054
Bst6I CTCTTC 2 cut(s) 384, 974
BstACI GRCGYC 1 cut(s) 1137
BstAPI GCANNNNNTGC 1 cut(s) 625
BstC8I GCNNGC 2 cut(s) 221, 441
BstDEI CTNAG 4 cut(s) 642, 804, 884, 997
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 3 cut(s) 88, 628, 871
BstFNI CGCG 1 cut(s) 675
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 5 cut(s) 304, 640, 795, 844, 1014
BstMAI GTCTC 3 cut(s) 37, 937, 1024
BstMBI GATC 5 cut(s) 301, 637, 792, 841, 1011
BstMCI CGRYCG 1 cut(s) 1018
BstMWI GCNNNNNNNGC 4 cut(s) 83, 380, 430, 625
BstNSI RCATGY 1 cut(s) 459
BstUI CGCG 1 cut(s) 675
BstV1I GCAGC 4 cut(s) 296, 892, 943, 983
BstV2I GAAGAC 2 cut(s) 192, 538
BstXI CCANNNNNNTGG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 870
BtgI CCRYGG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 926
BtsCI GGATG 3 cut(s) 88, 628, 871
BtsI GCAGTG 2 cut(s) 425, 434
BtsIMutI CAGTG 2 cut(s) 425, 434
Cac8I GCNNGC 2 cut(s) 221, 441
CaiI CAGNNNCTG 1 cut(s) 424
CfoI GCGC 1 cut(s) 675
Cfr13I GGNCC 4 cut(s) 209, 233, 275, 702
CpoI CGGWCCG 1 cut(s) 209
CseI GACGC 1 cut(s) 1126
CspI CGGWCCG 1 cut(s) 209
CviAII CATG 5 cut(s) 134, 239, 269, 456, 929
DdeI CTNAG 4 cut(s) 642, 804, 884, 997
DpnI GATC 5 cut(s) 303, 639, 794, 843, 1013
DpnII GATC 5 cut(s) 301, 637, 792, 841, 1011
DraIII CACNNNGTG 2 cut(s) 667, 965
DrdI GACNNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 384, 974
EarI CTCTTC 2 cut(s) 384, 974
Eco130I CCWWGG 1 cut(s) 238
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 4 cut(s) 209, 233, 275, 702
Eco57I CTGAAG 2 cut(s) 429, 858
Eco88I CYCGRG 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 5 cut(s) 137, 242, 272, 459, 932
FalI AAGNNNNNCTT 2 cut(s) 945, 977
FaqI GGGAC 2 cut(s) 219, 1124
FatI CATG 5 cut(s) 133, 238, 268, 455, 928
FauI CCCGC 2 cut(s) 499, 1150
FbaI TGATCA 1 cut(s) 301
Fnu4HI GCNGC 5 cut(s) 285, 881, 957, 972, 1135
FokI GGATG 3 cut(s) 95, 615, 878
Fsp4HI GCNGC 5 cut(s) 285, 881, 957, 972, 1135
FspBI CTAG 2 cut(s) 1070, 1153
GlaI GCGC 1 cut(s) 674
GluI GCNGC 5 cut(s) 285, 881, 957, 972, 1135
GsaI CCCAGC 1 cut(s) 81
HaeIII GGCC 1 cut(s) 870
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 1126
HhaI GCGC 1 cut(s) 675
Hin1I GRCGYC 1 cut(s) 1137
Hin1II CATG 5 cut(s) 137, 242, 272, 459, 932
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HinfI GANTC 6 cut(s) 203, 227, 316, 340, 512, 599
HpaII CCGG 1 cut(s) 207
HphI GGTGA 6 cut(s) 71, 155, 296, 358, 722, 1036
Hpy166II GTNNAC 2 cut(s) 485, 904
Hpy188I TCNGA 8 cut(s) 337, 645, 687, 733, 775, 865, 915, 1000
Hpy188III TCNNGA 8 cut(s) 151, 207, 278, 313, 322, 632, 893, 1040
Hpy8I GTNNAC 2 cut(s) 485, 904
Hpy99I CGWCG 1 cut(s) 1046
HpyAV CCTTC 4 cut(s) 50, 57, 673, 790
HpyCH4III ACNGT 4 cut(s) 36, 213, 547, 1054
HpyCH4IV ACGT 1 cut(s) 925
HpyCH4V TGCA 9 cut(s) 86, 455, 590, 619, 628, 823, 860, 938, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 380, 430, 625
HpyF3I CTNAG 4 cut(s) 642, 804, 884, 997
HpySE526I ACGT 1 cut(s) 925
Hsp92I GRCGYC 1 cut(s) 1137
Hsp92II CATG 5 cut(s) 137, 242, 272, 459, 932
HspAI GCGC 1 cut(s) 673
Kpn2I TCCGGA 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 5 cut(s) 301, 637, 792, 841, 1011
LmnI GCTCC 1 cut(s) 877
Lsp1109I GCAGC 4 cut(s) 296, 892, 943, 983
LweI GCATC 5 cut(s) 73, 637, 703, 869, 1117
MaeI CTAG 2 cut(s) 1070, 1153
MaeII ACGT 1 cut(s) 925
MalI GATC 5 cut(s) 303, 639, 794, 843, 1013
MboI GATC 5 cut(s) 301, 637, 792, 841, 1011
MboII GAAGA 6 cut(s) 34, 197, 401, 543, 851, 991
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 9 cut(s) 64, 146, 162, 175, 326, 395, 734, 933, 1119
MmeI TCCRAC 4 cut(s) 20, 162, 232, 711
MnlI CCTC 8 cut(s) 385, 604, 651, 759, 889, 1013, 1020, 1121
MroI TCCGGA 1 cut(s) 206
MseI TTAA 6 cut(s) 99, 138, 429, 738, 798, 1122
MslI CAYNNNNRTG 2 cut(s) 138, 633
MspA1I CMGCKG 2 cut(s) 356, 883
MspI CCGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 374
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 4 cut(s) 83, 380, 430, 625
NcoI CCATGG 1 cut(s) 238
NdeII GATC 5 cut(s) 301, 637, 792, 841, 1011
NlaIII CATG 5 cut(s) 137, 242, 272, 459, 932
NlaIV GGNNCC 2 cut(s) 33, 235
NspI RCATGY 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 1050
PctI GAATGC 1 cut(s) 374
PfeI GAWTC 6 cut(s) 203, 227, 316, 340, 512, 599
PflMI CCANNNNNTGG 1 cut(s) 929
PkrI GCNGC 5 cut(s) 286, 882, 958, 973, 1136
PpuMI RGGWCCY 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 233
PspFI CCCAGC 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 33, 235
PspPI GGNCC 4 cut(s) 209, 233, 275, 702
PspPPI RGGWCCY 1 cut(s) 233
PsrI GAACNNNNNNTAC 2 cut(s) 549, 581
PstNI CAGNNNCTG 1 cut(s) 424
PvuII CAGCTG 2 cut(s) 356, 883
RseI CAYNNNNRTG 2 cut(s) 138, 633
Rsr2I CGGWCCG 1 cut(s) 209
RsrII CGGWCCG 1 cut(s) 209
SaqAI TTAA 6 cut(s) 99, 138, 429, 738, 798, 1122
SatI GCNGC 5 cut(s) 285, 881, 957, 972, 1135
Sau3AI GATC 5 cut(s) 301, 637, 792, 841, 1011
Sau96I GGNCC 4 cut(s) 209, 233, 275, 702
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 5 cut(s) 73, 637, 703, 869, 1117
SinI GGWCC 4 cut(s) 209, 233, 275, 702
SmiMI CAYNNNNRTG 2 cut(s) 138, 633
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 9 cut(s) 64, 146, 162, 175, 326, 395, 734, 933, 1119
SsiI CCGC 5 cut(s) 273, 506, 521, 1134, 1143
SspI AATATT 1 cut(s) 816
SspMI CTAG 2 cut(s) 1070, 1153
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 4 cut(s) 36, 213, 547, 1054
TaiI ACGT 1 cut(s) 928
TaqI TCGA 4 cut(s) 94, 152, 225, 1014
TaqII GACCGA 1 cut(s) 1032
TasI AATT 9 cut(s) 64, 146, 162, 175, 326, 395, 734, 933, 1119
TauI GCSGC 1 cut(s) 1137
TfiI GAWTC 6 cut(s) 203, 227, 316, 340, 512, 599
Tru1I TTAA 6 cut(s) 99, 138, 429, 738, 798, 1122
Tru9I TTAA 6 cut(s) 99, 138, 429, 738, 798, 1122
TscAI CASTG 2 cut(s) 425, 441
TseI GCWGC 4 cut(s) 284, 880, 956, 971
TspDTI ATGAA 2 cut(s) 35, 671
TspRI CASTG 2 cut(s) 425, 441
Van91I CCANNNNNTGG 1 cut(s) 929
VpaK11BI GGWCC 4 cut(s) 209, 233, 275, 702
XapI RAATTY 5 cut(s) 64, 146, 326, 395, 734
XceI RCATGY 1 cut(s) 459
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XspI CTAG 2 cut(s) 1070, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.