pycom07g17570

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005979
Physical Location & Seq
Reverse (-)
20094576 .. 20097190
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17570.1

Sequence Viewer

Length: 1167 bp
ATGGTATCAGCTAAGTTAATGAGGCTTGTGTCACCTTGCTGGAAACCTTCTGTCGAGGGTGAAAATTCTAATAGGGGGGACGTGAGTGGTCGAATTGATGGTTTGTTGTGGCATAAAGATTCAGGACATCATGTTAATGGAGATTTCTCAATGGCAGTAATTCAAGCAAACAACTTGTTGGAAGACCATAGCCAACTAGAATCAGGGCCATTGAGCTCACTCGACTCGGGTCCTCATGGAACATTTGTTGGAATTTATGATGGACACGGAGGTCCAGAAACAGCCAGGTTTTTAAATGAACGTCTGTTCAATAACATCAAGACTTTCCGTGGTGCAGAGTTCACATCAGAGAATCAGGGAATGTCAGCTGATGTTATCACGAAAGCATTTTTGGCGACCGAAGAGGAGTTCCTCTCTCTAGTAAAGAAGCAGTGGACAATCAAGCCACTGCTTGCTTCTGTCGGTGCATGTTGTTTGGTAGGCATAGTATGTAGTGGGCTGCTATACATAGCAAATGCGGGGGATTCTCGTGTGGTGCTAGGAAAACTGGAAAAGACCGTTAAACAGGTAAAAGCGGTTCAGCTATCGATTGAGCACAATGCAAGTTATGAATCTGTGAGAGAGGAGTTGCGTTCGTTGCATCCCGATGATCCACAGATTGTGTTATTAAAGCACAAAGTGTGGCGCGTGAAGGGTTTGATACAGATTTCAAGATCCATCGGTGCTGCCTACCTAAAGAGGCAAGAATTTAACAAAGAACCTCTATTGGCAAAGTTCAGATTATCACAACCCTTCAACAAGCCGATCCTTAAAGCTGAGCCAACAATATTAGTCCAAAAACTCTACCCCGAAGATCAGTTTCTCATATTTGCTTCAGACGGCCTATGGGAGCAGTTAAGCAATCAGGAGGCAGTTGACATTGTCCAGAGTTATCCGCGTAATGGTAGCGCAAGGAAACTTGTCAAAGCTGCACTTCGCGAAGCAGCGAAGAAGAGAGAAATGAGATATTCGGACTTAAAAAAGATAGACCGAGGGGTGAGGAGACATTTTCACGACGACATCACAGTGGTAGTTCTGTTCCTAGATTCGCATCTGGTCAGTCGTAGCTACTGCCACGGGCCTCTGCTATCGGTTAAGGCAGCTGGTGGTGTCCCTGCCGGCACCTAG

Protein Analysis

389

Amino Acids

43.05

Weight (kDa)

9.02

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 71 - 329 1.1e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 1160
AccII CGCG 3 cut(s) 687, 937, 978
AciI CCGC 3 cut(s) 518, 575, 935
AclWI GGATC 3 cut(s) 644, 708, 799
AcsI RAATTY 3 cut(s) 64, 252, 746
AcuI CTGAAG 1 cut(s) 858
AdeI CACNNNGTG 1 cut(s) 679
AfiI CCNNNNNNNGG 1 cut(s) 941
AgsI TTSAA 4 cut(s) 164, 310, 711, 796
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 284
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 8 cut(s) 11, 216, 368, 583, 815, 968, 1107, 1142
AluI AGCT 8 cut(s) 11, 216, 368, 583, 815, 968, 1107, 1142
Alw21I GWGCWC 2 cut(s) 218, 597
Alw26I GTCTC 1 cut(s) 1036
AlwI GGATC 3 cut(s) 644, 708, 799
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 3 cut(s) 206, 880, 1118
ApeKI GCWGC 5 cut(s) 499, 725, 968, 983, 1139
ApoI RAATTY 3 cut(s) 64, 252, 746
AspLEI GCGC 2 cut(s) 687, 950
AspS9I GGNCC 4 cut(s) 206, 230, 272, 1118
AsuHPI GGTGA 3 cut(s) 24, 71, 1048
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 2 cut(s) 230, 272
BanI GGYRCC 1 cut(s) 1160
BanII GRGCYC 1 cut(s) 218
BauI CACGAG 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 2 cut(s) 218, 597
BbvI GCAGC 5 cut(s) 486, 712, 955, 995, 1151
BccI CCATC 3 cut(s) 92, 254, 725
BceAI ACGGC 1 cut(s) 895
BciT130I CCWGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 1036
BfaI CTAG 5 cut(s) 197, 419, 539, 1082, 1165
BisI GCNGC 5 cut(s) 500, 726, 969, 984, 1140
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 5 cut(s) 501, 727, 970, 985, 1141
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 2 cut(s) 230, 272
BmeT110I CYCGRG 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 206, 230, 272, 1118
BmiI GGNNCC 2 cut(s) 231, 1162
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 649, 1099
BoxI GACNNNNGTC 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 189
BplI GAGNNNNNCTC 2 cut(s) 398, 430
Bpu1102I GCTNAGC 1 cut(s) 816
Bsa29I ATCGAT 1 cut(s) 587
BsaBI GATNNNNATC 1 cut(s) 1089
BsaJI CCNNGG 3 cut(s) 328, 1030, 1114
Bsc4I CCNNNNNNNGG 1 cut(s) 941
Bse118I RCCGGY 1 cut(s) 1157
Bse1I ACTGG 1 cut(s) 552
Bse8I GATNNNNATC 1 cut(s) 1089
BseBI CCWGG 1 cut(s) 286
BseCI ATCGAT 1 cut(s) 587
BseDI CCNNGG 3 cut(s) 328, 1030, 1114
BseGI GGATG 1 cut(s) 640
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 1 cut(s) 941
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 3 cut(s) 419, 638, 1054
BseXI GCAGC 5 cut(s) 486, 712, 955, 995, 1151
BsgI GTGCAG 2 cut(s) 354, 954
Bsh1236I CGCG 3 cut(s) 687, 937, 978
Bsh1285I CGRYCG 1 cut(s) 399
BshFI GGCC 3 cut(s) 208, 882, 1120
BshNI GGYRCC 1 cut(s) 1160
BshVI ATCGAT 1 cut(s) 587
BsiEI CGRYCG 1 cut(s) 399
BsiHKAI GWGCWC 2 cut(s) 218, 597
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 1 cut(s) 1158
BslFI GGGAC 2 cut(s) 92, 1136
BslI CCNNNNNNNGG 1 cut(s) 941
BsmAI GTCTC 1 cut(s) 1036
BsmFI GGGAC 2 cut(s) 92, 1136
BsnI GGCC 3 cut(s) 208, 882, 1120
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 2 cut(s) 218, 597
Bsp143I GATC 4 cut(s) 649, 713, 804, 853
Bsp1720I GCTNAGC 1 cut(s) 816
Bsp68I TCGCGA 1 cut(s) 978
BspACI CCGC 3 cut(s) 518, 575, 935
BspANI GGCC 3 cut(s) 208, 882, 1120
BspCNI CTCAG 1 cut(s) 808
BspDI ATCGAT 1 cut(s) 587
BspFNI CGCG 3 cut(s) 687, 937, 978
BspLI GGNNCC 2 cut(s) 231, 1162
BspPI GGATC 3 cut(s) 644, 708, 799
BspT107I GGYRCC 1 cut(s) 1160
BsrFI RCCGGY 1 cut(s) 1157
BsrI ACTGG 1 cut(s) 552
BssAI RCCGGY 1 cut(s) 1157
BssECI CCNNGG 3 cut(s) 328, 1030, 1114
BssMI GATC 4 cut(s) 649, 713, 804, 853
BssSI CACGAG 1 cut(s) 528
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 2 cut(s) 559, 1066
Bst6I CTCTTC 2 cut(s) 396, 986
BstC8I GCNNGC 2 cut(s) 453, 1159
BstDEI CTNAG 2 cut(s) 12, 816
BstDSI CCRYGG 2 cut(s) 328, 1114
BstF5I GGATG 1 cut(s) 640
BstFNI CGCG 3 cut(s) 687, 937, 978
BstHHI GCGC 2 cut(s) 687, 950
BstKTI GATC 4 cut(s) 652, 716, 807, 856
BstMAI GTCTC 1 cut(s) 1036
BstMBI GATC 4 cut(s) 649, 713, 804, 853
BstMCI CGRYCG 1 cut(s) 399
BstMWI GCNNNNNNNGC 2 cut(s) 392, 637
BstNI CCWGG 1 cut(s) 286
BstNSI RCATGY 1 cut(s) 471
BstPAI GACNNNNGTC 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 284
BstUI CGCG 3 cut(s) 687, 937, 978
BstV1I GCAGC 5 cut(s) 486, 712, 955, 995, 1151
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 1 cut(s) 713
BstYI RGATCY 1 cut(s) 713
Bsu15I ATCGAT 1 cut(s) 587
BsuRI GGCC 3 cut(s) 208, 882, 1120
BsuTUI ATCGAT 1 cut(s) 587
BtgI CCRYGG 2 cut(s) 328, 1114
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 640
BtsI GCAGTG 2 cut(s) 437, 446
BtsIMutI CAGTG 3 cut(s) 437, 446, 1071
BtuMI TCGCGA 1 cut(s) 978
Cac8I GCNNGC 2 cut(s) 453, 1159
CfoI GCGC 2 cut(s) 687, 950
Cfr10I RCCGGY 1 cut(s) 1157
Cfr13I GGNCC 4 cut(s) 206, 230, 272, 1118
ClaI ATCGAT 1 cut(s) 587
CviAII CATG 3 cut(s) 131, 236, 468
DdeI CTNAG 2 cut(s) 12, 816
DpnI GATC 4 cut(s) 651, 715, 806, 855
DpnII GATC 4 cut(s) 649, 713, 804, 853
DraI TTTAAA 1 cut(s) 294
DraIII CACNNNGTG 1 cut(s) 679
DrdI GACNNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 270
Eam1104I CTCTTC 2 cut(s) 396, 986
EarI CTCTTC 2 cut(s) 396, 986
Ecl136II GAGCTC 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 230, 272
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 858
Eco88I CYCGRG 1 cut(s) 226
EcoICRI GAGCTC 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 284
EcoT38I GRGCYC 1 cut(s) 218
FaeI CATG 3 cut(s) 134, 239, 471
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FaqI GGGAC 2 cut(s) 92, 1136
FatI CATG 3 cut(s) 130, 235, 467
FauI CCCGC 1 cut(s) 511
Fnu4HI GCNGC 5 cut(s) 500, 726, 969, 984, 1140
FokI GGATG 1 cut(s) 627
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 5 cut(s) 500, 726, 969, 984, 1140
FspBI CTAG 5 cut(s) 197, 419, 539, 1082, 1165
GlaI GCGC 2 cut(s) 686, 949
GluI GCNGC 5 cut(s) 500, 726, 969, 984, 1140
HaeIII GGCC 3 cut(s) 208, 882, 1120
HapII CCGG 1 cut(s) 1158
HhaI GCGC 2 cut(s) 687, 950
Hin1II CATG 3 cut(s) 134, 239, 471
Hin6I GCGC 2 cut(s) 685, 948
HinP1I GCGC 2 cut(s) 685, 948
HincII GTYRAC 1 cut(s) 916
HindII GTYRAC 1 cut(s) 916
HinfI GANTC 7 cut(s) 119, 200, 224, 352, 524, 611, 1085
HpaII CCGG 1 cut(s) 1158
HphI GGTGA 3 cut(s) 24, 71, 1048
Hpy166II GTNNAC 3 cut(s) 342, 435, 916
Hpy188I TCNGA 4 cut(s) 349, 779, 877, 1012
Hpy8I GTNNAC 3 cut(s) 342, 435, 916
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 3 cut(s) 57, 685, 802
HpyCH4III ACNGT 2 cut(s) 559, 1066
HpyCH4IV ACGT 2 cut(s) 81, 301
HpyCH4V TGCA 5 cut(s) 335, 467, 602, 640, 971
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 637
HpyF3I CTNAG 2 cut(s) 12, 816
HpySE526I ACGT 2 cut(s) 81, 301
Hsp92II CATG 3 cut(s) 134, 239, 471
HspAI GCGC 2 cut(s) 685, 948
KroI GCCGGC 1 cut(s) 1157
KroNI GCCGGC 1 cut(s) 1159
Kzo9I GATC 4 cut(s) 649, 713, 804, 853
LmnI GCTCC 1 cut(s) 889
Lsp1109I GCAGC 5 cut(s) 486, 712, 955, 995, 1151
LweI GCATC 2 cut(s) 649, 1099
MaeI CTAG 5 cut(s) 197, 419, 539, 1082, 1165
MaeII ACGT 2 cut(s) 81, 301
MaeIII GTNAC 1 cut(s) 30
MalI GATC 4 cut(s) 651, 715, 806, 855
MboI GATC 4 cut(s) 649, 713, 804, 853
MboII GAAGA 5 cut(s) 194, 413, 863, 1000, 1003
MflI RGATCY 1 cut(s) 713
MhlI GDGCHC 2 cut(s) 218, 597
MluCI AATT 5 cut(s) 64, 93, 159, 252, 746
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 2 cut(s) 159, 229
MroNI GCCGGC 1 cut(s) 1157
MslI CAYNNNNRTG 3 cut(s) 135, 645, 1064
MspA1I CMGCKG 2 cut(s) 368, 1142
MspI CCGG 1 cut(s) 1158
MspR9I CCNGG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 286
MvnI CGCG 3 cut(s) 687, 937, 978
MwoI GCNNNNNNNGC 2 cut(s) 392, 637
NaeI GCCGGC 1 cut(s) 1159
NdeII GATC 4 cut(s) 649, 713, 804, 853
NgoMIV GCCGGC 1 cut(s) 1157
NlaIII CATG 3 cut(s) 134, 239, 471
NlaIV GGNNCC 2 cut(s) 231, 1162
NmuCI GTSAC 1 cut(s) 30
NruI TCGCGA 1 cut(s) 978
NspI RCATGY 1 cut(s) 471
PdiI GCCGGC 1 cut(s) 1159
PfeI GAWTC 6 cut(s) 119, 200, 352, 524, 611, 1085
PflFI GACNNNGTC 1 cut(s) 920
PkrI GCNGC 5 cut(s) 501, 727, 970, 985, 1141
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PpuMI RGGWCCY 1 cut(s) 230
PshAI GACNNNNGTC 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 2 cut(s) 231, 1162
PspPI GGNCC 4 cut(s) 206, 230, 272, 1118
PspPPI RGGWCCY 1 cut(s) 230
PsrI GAACNNNNNNTAC 2 cut(s) 561, 593
PsuI RGATCY 1 cut(s) 713
PsyI GACNNNGTC 1 cut(s) 920
PvuII CAGCTG 2 cut(s) 368, 1142
RruI TCGCGA 1 cut(s) 978
RseI CAYNNNNRTG 3 cut(s) 135, 645, 1064
SacI GAGCTC 1 cut(s) 218
SatI GCNGC 5 cut(s) 500, 726, 969, 984, 1140
Sau3AI GATC 4 cut(s) 649, 713, 804, 853
Sau96I GGNCC 4 cut(s) 206, 230, 272, 1118
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 2 cut(s) 218, 597
SfaNI GCATC 2 cut(s) 649, 1099
SinI GGWCC 2 cut(s) 230, 272
SmiMI CAYNNNNRTG 3 cut(s) 135, 645, 1064
Sse9I AATT 5 cut(s) 64, 93, 159, 252, 746
SsiI CCGC 3 cut(s) 518, 575, 935
SspI AATATT 1 cut(s) 828
SspMI CTAG 5 cut(s) 197, 419, 539, 1082, 1165
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 284
TaaI ACNGT 2 cut(s) 559, 1066
TaiI ACGT 2 cut(s) 84, 304
TaqI TCGA 4 cut(s) 54, 91, 222, 587
TaqII GACCGA 2 cut(s) 413, 1044
TasI AATT 5 cut(s) 64, 93, 159, 252, 746
TfiI GAWTC 6 cut(s) 119, 200, 352, 524, 611, 1085
TscAI CASTG 3 cut(s) 437, 453, 1071
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 5 cut(s) 499, 725, 968, 983, 1139
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 2 cut(s) 312, 624
TspGWI ACGGA 2 cut(s) 282, 317
TspRI CASTG 3 cut(s) 437, 453, 1071
Tth111I GACNNNGTC 1 cut(s) 920
VpaK11BI GGWCC 2 cut(s) 230, 272
XapI RAATTY 3 cut(s) 64, 252, 746
XceI RCATGY 1 cut(s) 471
XspI CTAG 5 cut(s) 197, 419, 539, 1082, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.