MD01G1115100.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005979
Physical Location & Seq
Reverse (-)
22924881 .. 22928714
3834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1115100.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGGTATCAACTACTTTTCTGAAGCTTGTGTCACCTTGCTGGAAACCGTCTTTCGAGGGTGAAAATTCTAATAGGGGGGACGTGAGTGGTCGAATTGATGGTTTGTTGTGGTATAAAGACTCAGGGCATCATGTTAATGGAGATTTCTCGATGGCAGTAATTCAAGCAAACAATTTGTTGGAAGACCATAGCCAACTGGAATCGGGGCCATTGAGCTCCCTCGAATCAGGTCCTCATGGAACATTTGTTGGAATTTATGATGGACACGGAGGTCCAGAAACAGCCCGGTTTTTAAATGAAAACCTGTTCAACAATATCAAGAAGTTCACGTCAGAGAATCAGGGAATGTCAGCTGATGTTATCACCAAGGCATTTTTGGCGACCGAAGAGGAGTTCCTCTCTCTAGTAAAGAAGCAGTGGGCAATCAAGCCACTACTTGCTTCTGTTGGTGCATGTTGTTTAGTAGGCATAGTATGTAGTGGGCTGCTATACATAGCAAATGCGGGGGATTCTCGTGTTGTGCTAGGAAGACTGGAAAAGACCATTAAACAGGTAAAAGCAGTTCAGTTATCAATCGAGCACAATGCAAGTCATGAATCTGTGAGAGAGGAGTTGCACTCGTTGCATCCTGATGATCCACAGATTGTGATGTTAAAGCACAAGGTGTGGCGCGTGAAGGGTTTGATACAGGTTTCAAGATCCATCGGCGATGCCTACCTGAAGAGGCAAGAGTTTAACAAAGAACCTCTATTGGCAAAGTTCAGATTATCACAACCCTTCAACAAGCCGATCCTTAAAGCTGAGCCAACAATCCTAGTCCAAAAACTCTACCCGGAAGATCAGTTTCTCATATTTGCATCAGACGGCCTATGGGAGCAGTTAAGCAATCAGGACGCAGTTGACATTGTCCAGAGCTATCCGCGTAATGGTATTGCAAGGAAACTTGTGAAAGCCGCGCTTCATGAAGCAGCAAAGAAGAGAGAAATGAGATATTCGGACTTAAAAAAGATAGACCGAGGGGTGAGGAGACATTTTCACGACGACATCACAGTGGTAGTTCTGTTCCTAGATTCGCATCTGGTCAGTCATAGCTACGGGCACGGGCCTCTGCTTTCGGTTAAGGCAGGTGGTGGTGTCCCTGCCACCAGCACCTAG

Protein Analysis

385

Amino Acids

42.61

Weight (kDa)

8.22

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 71 - 325 2.6e-37 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1115
AasI GACNNNNNNGTC 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 1115
AccII CGCG 3 cut(s) 672, 922, 956
AciI CCGC 3 cut(s) 503, 920, 954
AclWI GGATC 3 cut(s) 629, 693, 784
AcsI RAATTY 2 cut(s) 64, 252
AcuI CTGAAG 2 cut(s) 41, 740
AdeI CACNNNGTG 1 cut(s) 664
AfiI CCNNNNNNNGG 1 cut(s) 926
AgsI TTSAA 4 cut(s) 164, 310, 696, 781
AjiI CACGTC 2 cut(s) 82, 330
AluBI AGCT 6 cut(s) 25, 216, 353, 800, 915, 1092
AluI AGCT 6 cut(s) 25, 216, 353, 800, 915, 1092
Alw21I GWGCWC 2 cut(s) 218, 582
Alw26I GTCTC 1 cut(s) 1021
AlwI GGATC 3 cut(s) 629, 693, 784
AoxI GGCC 3 cut(s) 206, 865, 1103
ApeKI GCWGC 2 cut(s) 484, 968
ApoI RAATTY 2 cut(s) 64, 252
AspLEI GCGC 2 cut(s) 672, 958
AspS9I GGNCC 4 cut(s) 206, 230, 272, 1103
AsuC2I CCSGG 2 cut(s) 286, 833
AsuHPI GGTGA 4 cut(s) 24, 71, 355, 1033
AvaII GGWCC 2 cut(s) 230, 272
BaeGI GKGCMC 1 cut(s) 1101
BanII GRGCYC 1 cut(s) 218
BauI CACGAG 1 cut(s) 513
BbsI GAAGAC 2 cut(s) 189, 535
Bbv12I GWGCWC 2 cut(s) 218, 582
BbvI GCAGC 2 cut(s) 471, 980
BccI CCATC 4 cut(s) 92, 145, 254, 710
BceAI ACGGC 1 cut(s) 880
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BcnI CCSGG 2 cut(s) 286, 833
BcoDI GTCTC 1 cut(s) 1021
BfaI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
BfuAI ACCTGC 1 cut(s) 1115
BisI GCNGC 3 cut(s) 485, 954, 969
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 3 cut(s) 486, 955, 970
Bme1390I CCNGG 2 cut(s) 286, 833
Bme18I GGWCC 2 cut(s) 230, 272
BmgBI CACGTC 2 cut(s) 82, 330
BmgT120I GGNCC 4 cut(s) 206, 230, 272, 1103
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 286, 833
BmsI GCATC 5 cut(s) 136, 634, 700, 866, 1084
BpiI GAAGAC 2 cut(s) 189, 535
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 602, 634
Bpu1102I GCTNAGC 1 cut(s) 801
BpuMI CCSGG 2 cut(s) 286, 833
BsaBI GATNNNNATC 1 cut(s) 1074
BsaJI CCNNGG 2 cut(s) 366, 1015
Bsc4I CCNNNNNNNGG 1 cut(s) 926
Bse1I ACTGG 2 cut(s) 201, 537
Bse8I GATNNNNATC 1 cut(s) 1074
BseDI CCNNGG 2 cut(s) 366, 1015
BseGI GGATG 1 cut(s) 625
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 1 cut(s) 926
BseMII CTCAG 2 cut(s) 135, 792
BseNI ACTGG 2 cut(s) 201, 537
BseRI GAGGAG 3 cut(s) 404, 623, 1039
BseSI GKGCMC 1 cut(s) 1101
BseXI GCAGC 2 cut(s) 471, 980
Bsh1236I CGCG 3 cut(s) 672, 922, 956
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 3 cut(s) 208, 867, 1105
BsiEI CGRYCG 1 cut(s) 384
BsiHKAI GWGCWC 2 cut(s) 218, 582
BsiSI CCGG 2 cut(s) 286, 833
BslFI GGGAC 2 cut(s) 92, 1121
BslI CCNNNNNNNGG 1 cut(s) 926
BsmAI GTCTC 1 cut(s) 1021
BsmFI GGGAC 2 cut(s) 92, 1121
BsnI GGCC 3 cut(s) 208, 867, 1105
Bsp1286I GDGCHC 3 cut(s) 218, 582, 1101
Bsp143I GATC 4 cut(s) 634, 698, 789, 838
Bsp1720I GCTNAGC 1 cut(s) 801
BspACI CCGC 3 cut(s) 503, 920, 954
BspANI GGCC 3 cut(s) 208, 867, 1105
BspCNI CTCAG 2 cut(s) 134, 793
BspFNI CGCG 3 cut(s) 672, 922, 956
BspHI TCATGA 2 cut(s) 592, 961
BspLI GGNNCC 1 cut(s) 207
BspMI ACCTGC 1 cut(s) 1115
BspPI GGATC 3 cut(s) 629, 693, 784
BsrI ACTGG 2 cut(s) 201, 537
BssECI CCNNGG 2 cut(s) 366, 1015
BssMI GATC 4 cut(s) 634, 698, 789, 838
BssSI CACGAG 1 cut(s) 513
BssT1I CCWWGG 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 48, 1051
Bst6I CTCTTC 3 cut(s) 381, 716, 971
BstAPI GCANNNNNTGC 1 cut(s) 622
BstDEI CTNAG 2 cut(s) 121, 801
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 3 cut(s) 672, 922, 956
BstHHI GCGC 2 cut(s) 672, 958
BstKTI GATC 4 cut(s) 637, 701, 792, 841
BstMAI GTCTC 1 cut(s) 1021
BstMBI GATC 4 cut(s) 634, 698, 789, 838
BstMCI CGRYCG 1 cut(s) 384
BstMWI GCNNNNNNNGC 2 cut(s) 377, 622
BstNSI RCATGY 1 cut(s) 456
BstSCI CCNGG 2 cut(s) 284, 831
BstSLI GKGCMC 1 cut(s) 1101
BstUI CGCG 3 cut(s) 672, 922, 956
BstV1I GCAGC 2 cut(s) 471, 980
BstV2I GAAGAC 2 cut(s) 189, 535
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
BsuRI GGCC 3 cut(s) 208, 867, 1105
BtgZI GCGATG 1 cut(s) 723
BtrI CACGTC 2 cut(s) 82, 330
BtsCI GGATG 1 cut(s) 625
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 2 cut(s) 422, 1056
BveI ACCTGC 1 cut(s) 1115
CciI TCATGA 2 cut(s) 592, 961
CfoI GCGC 2 cut(s) 672, 958
Cfr13I GGNCC 4 cut(s) 206, 230, 272, 1103
CseI GACGC 1 cut(s) 902
CviAII CATG 5 cut(s) 131, 236, 453, 593, 962
DdeI CTNAG 2 cut(s) 121, 801
DpnI GATC 4 cut(s) 636, 700, 791, 840
DpnII GATC 4 cut(s) 634, 698, 789, 838
DraI TTTAAA 1 cut(s) 294
DraIII CACNNNGTG 1 cut(s) 664
DrdI GACNNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 270
Eam1104I CTCTTC 3 cut(s) 381, 716, 971
EarI CTCTTC 3 cut(s) 381, 716, 971
Ecl136II GAGCTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 230, 272
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 2 cut(s) 41, 740
EcoICRI GAGCTC 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 218
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 5 cut(s) 134, 239, 456, 596, 965
FalI AAGNNNNNCTT 2 cut(s) 942, 974
FaqI GGGAC 2 cut(s) 92, 1121
FatI CATG 5 cut(s) 130, 235, 452, 592, 961
FauI CCCGC 1 cut(s) 496
Fnu4HI GCNGC 3 cut(s) 485, 954, 969
FokI GGATG 1 cut(s) 612
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 3 cut(s) 485, 954, 969
FspBI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
GlaI GCGC 2 cut(s) 671, 957
GluI GCNGC 3 cut(s) 485, 954, 969
HaeIII GGCC 3 cut(s) 208, 867, 1105
HapII CCGG 2 cut(s) 286, 833
HgaI GACGC 1 cut(s) 902
HhaI GCGC 2 cut(s) 672, 958
Hin1II CATG 5 cut(s) 134, 239, 456, 596, 965
Hin6I GCGC 2 cut(s) 670, 956
HinP1I GCGC 2 cut(s) 670, 956
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 7 cut(s) 119, 200, 224, 337, 509, 596, 1070
HpaII CCGG 2 cut(s) 286, 833
HphI GGTGA 4 cut(s) 24, 71, 355, 1033
Hpy166II GTNNAC 2 cut(s) 327, 901
Hpy188I TCNGA 5 cut(s) 21, 334, 764, 862, 997
Hpy8I GTNNAC 2 cut(s) 327, 901
Hpy99I CGWCG 1 cut(s) 1043
HpyAV CCTTC 2 cut(s) 670, 787
HpyCH4III ACNGT 2 cut(s) 48, 1051
HpyCH4IV ACGT 2 cut(s) 81, 329
HpyCH4V TGCA 6 cut(s) 452, 587, 616, 625, 857, 935
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 622
HpyF3I CTNAG 2 cut(s) 121, 801
HpySE526I ACGT 2 cut(s) 81, 329
Hsp92II CATG 5 cut(s) 134, 239, 456, 596, 965
HspAI GCGC 2 cut(s) 670, 956
Kzo9I GATC 4 cut(s) 634, 698, 789, 838
LmnI GCTCC 2 cut(s) 221, 874
Lsp1109I GCAGC 2 cut(s) 471, 980
LweI GCATC 5 cut(s) 136, 634, 700, 866, 1084
MaeI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
MaeII ACGT 2 cut(s) 81, 329
MaeIII GTNAC 1 cut(s) 30
MalI GATC 4 cut(s) 636, 700, 791, 840
MboI GATC 4 cut(s) 634, 698, 789, 838
MboII GAAGA 6 cut(s) 194, 398, 540, 733, 848, 988
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 3 cut(s) 218, 582, 1101
MluCI AATT 5 cut(s) 64, 93, 159, 172, 252
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 2 cut(s) 159, 229
MseI TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
MslI CAYNNNNRTG 3 cut(s) 135, 630, 1049
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 286, 833
MspR9I CCNGG 2 cut(s) 286, 833
MvnI CGCG 3 cut(s) 672, 922, 956
MwoI GCNNNNNNNGC 2 cut(s) 377, 622
NciI CCSGG 2 cut(s) 286, 833
NdeII GATC 4 cut(s) 634, 698, 789, 838
NlaIII CATG 5 cut(s) 134, 239, 456, 596, 965
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 30
NspI RCATGY 1 cut(s) 456
PagI TCATGA 2 cut(s) 592, 961
PaqCI CACCTGC 1 cut(s) 1115
PfeI GAWTC 6 cut(s) 200, 224, 337, 509, 596, 1070
PflFI GACNNNGTC 1 cut(s) 905
PkrI GCNGC 3 cut(s) 486, 955, 970
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 4 cut(s) 206, 230, 272, 1103
PspPPI RGGWCCY 1 cut(s) 230
PsrI GAACNNNNNNTAC 2 cut(s) 546, 578
PsuI RGATCY 1 cut(s) 698
PsyI GACNNNGTC 1 cut(s) 905
PvuII CAGCTG 1 cut(s) 353
RseI CAYNNNNRTG 3 cut(s) 135, 630, 1049
SacI GAGCTC 1 cut(s) 218
SaqAI TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
SatI GCNGC 3 cut(s) 485, 954, 969
Sau3AI GATC 4 cut(s) 634, 698, 789, 838
Sau96I GGNCC 4 cut(s) 206, 230, 272, 1103
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 2 cut(s) 286, 833
SduI GDGCHC 3 cut(s) 218, 582, 1101
SfaNI GCATC 5 cut(s) 136, 634, 700, 866, 1084
SinI GGWCC 2 cut(s) 230, 272
SmiMI CAYNNNNRTG 3 cut(s) 135, 630, 1049
Sse9I AATT 5 cut(s) 64, 93, 159, 172, 252
SsiI CCGC 3 cut(s) 503, 920, 954
SspMI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 2 cut(s) 284, 831
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 48, 1051
TaiI ACGT 2 cut(s) 84, 332
TaqI TCGA 5 cut(s) 54, 91, 149, 222, 576
TaqII GACCGA 2 cut(s) 398, 1029
TasI AATT 5 cut(s) 64, 93, 159, 172, 252
TauI GCSGC 1 cut(s) 956
TfiI GAWTC 6 cut(s) 200, 224, 337, 509, 596, 1070
Tru1I TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
Tru9I TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
TscAI CASTG 2 cut(s) 422, 1056
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 2 cut(s) 484, 968
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 4 cut(s) 312, 609, 950, 978
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 2 cut(s) 422, 1056
Tth111I GACNNNGTC 1 cut(s) 905
VpaK11BI GGWCC 2 cut(s) 230, 272
XapI RAATTY 2 cut(s) 64, 252
XceI RCATGY 1 cut(s) 456
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XspI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.