pycom01g14400

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005979
Physical Location & Seq
Reverse (-)
14573760 .. 14576343
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14400.1

Sequence Viewer

Length: 1155 bp
ATGGTATCAACTACTTTTCTGAAACTTGTGTCACCTTGCTGGAAACCATCTTTCGAGGGTGAAAATTCTAATAGGGGGGACGTGAGTGGTCGAATTGATGGTTTGTTGTGGCATAAAGATTCAGGGCATCATGTTAATGGAGATTTCTCGATGGCAGTAATTCAAGCAAACAATTTGTTGGAAGACCATAGCCAACTGGAATCGGGACCATTGAGCTCCCTCGAATCCGGTCCTCATGGAACATTTGTTGGAATTTATGATGGACACGGAGGTCCAGAAACAGCCCGGTTTTTAAACGAACACCTGTTCAACAATATCAAGAAGTTCACATCAGAGAATCAAGGAATGTCAGCCGATGTTATCACCAAAGCATTTTTGGCAACCGAAGAGGAGTTCCTCTCTCTAGTAAAGAAGCAGTGGGCAATCAAGCCACTGCTTGCTTCTGTTGGTGCATGTTGTTTAGTAGGCATAGTATGTAGTGGGCTGCTATACATAGCAAATGCGGGGGATTCTCGTGTGGTGCTAGGAAGACTGGAAAAGACCGTTAAGCAGGTAAAAGCAGTTCAGTTATCGATTGAGCACAATGCAAGTCATGAATCTGTGAGAGAGGAGTTGCACTCGTTGCATCCCGATGATCCACAGATTGTGATGTTAAAGCACAAGGTGTGGCGCGTGAAGGGTTTGATACAGGTTTCAAGATCCATCGGCGATGCCTACCTGAAGAGGCAAGAATTTAACAAAGAACCTCTATTGGCAAAGTTCAGATTATCACAACCCTTCAACAAGCCGATCCTTAAAGCTGAGCCAACTATCCTAGTCCAAAAACTCTACCCTGAGGATCAGTTTCTCATATTTGCATCAGACGGCCTATGGGAGCAGTTAAGCAATCAGGACGCAGTTGACATTGTCCAGAGCTATCCGCGTAATGGTATTGCAAGGAAACTTGTGAAAGCAGCGCTTCGTGAAGCAGCAAAGAAGAGAGAAATGAGATATTCGGACTTAAAAAAGATAGACCGAGGGGTGAGGAGACATTTTCACGACGACATCACAGTGGTAGTTCTGTTCCTAGATTCGCATCTGGTCAGTCATAGCTACGGGCACGGGCCTCTGCTTTCCGTTAAGGCAGGTGGTGGTGTCCCTGCCACCGGCACCTAG

Protein Analysis

385

Amino Acids

42.58

Weight (kDa)

8.51

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 71 - 325 1.4e-37 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1115
AasI GACNNNNNNGTC 1 cut(s) 270
Acc36I ACCTGC 2 cut(s) 541, 1115
AccB1I GGYRCC 1 cut(s) 1148
AccII CGCG 2 cut(s) 672, 922
AciI CCGC 2 cut(s) 503, 920
AclWI GGATC 4 cut(s) 629, 693, 784, 846
AcsI RAATTY 3 cut(s) 64, 252, 731
AcuI CTGAAG 1 cut(s) 740
AdeI CACNNNGTG 1 cut(s) 664
AfeI AGCGCT 1 cut(s) 957
AfiI CCNNNNNNNGG 2 cut(s) 926, 1145
AgsI TTSAA 4 cut(s) 164, 310, 696, 781
AjiI CACGTC 1 cut(s) 82
AluBI AGCT 4 cut(s) 216, 800, 915, 1092
AluI AGCT 4 cut(s) 216, 800, 915, 1092
Alw21I GWGCWC 2 cut(s) 218, 582
Alw26I GTCTC 1 cut(s) 1021
AlwI GGATC 4 cut(s) 629, 693, 784, 846
Aor51HI AGCGCT 1 cut(s) 957
AoxI GGCC 2 cut(s) 865, 1103
ApeKI GCWGC 3 cut(s) 484, 953, 968
ApoI RAATTY 3 cut(s) 64, 252, 731
AspLEI GCGC 2 cut(s) 672, 958
AspS9I GGNCC 4 cut(s) 206, 230, 272, 1103
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 4 cut(s) 24, 71, 355, 1033
AvaII GGWCC 3 cut(s) 206, 230, 272
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 1 cut(s) 1101
BanI GGYRCC 1 cut(s) 1148
BanII GRGCYC 1 cut(s) 218
BauI CACGAG 1 cut(s) 513
BbsI GAAGAC 2 cut(s) 189, 535
Bbv12I GWGCWC 2 cut(s) 218, 582
BbvI GCAGC 3 cut(s) 471, 965, 980
BccI CCATC 5 cut(s) 55, 92, 145, 254, 710
BceAI ACGGC 1 cut(s) 880
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 1021
BfaI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
BfoI RGCGCY 1 cut(s) 959
BfuAI ACCTGC 2 cut(s) 541, 1115
BisI GCNGC 3 cut(s) 485, 954, 969
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 3 cut(s) 486, 955, 970
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 3 cut(s) 206, 230, 272
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 206, 230, 272, 1103
BmiI GGNNCC 2 cut(s) 207, 1150
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 5 cut(s) 136, 634, 700, 866, 1084
BpiI GAAGAC 2 cut(s) 189, 535
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 602, 634
Bpu1102I GCTNAGC 1 cut(s) 801
BpuMI CCSGG 1 cut(s) 286
Bsa29I ATCGAT 1 cut(s) 572
BsaBI GATNNNNATC 1 cut(s) 1074
BsaJI CCNNGG 1 cut(s) 1015
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 2 cut(s) 926, 1145
Bse118I RCCGGY 1 cut(s) 1145
Bse1I ACTGG 2 cut(s) 201, 537
Bse21I CCTNAGG 1 cut(s) 834
Bse8I GATNNNNATC 1 cut(s) 1074
BseCI ATCGAT 1 cut(s) 572
BseDI CCNNGG 1 cut(s) 1015
BseGI GGATG 1 cut(s) 625
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 2 cut(s) 926, 1145
BseMII CTCAG 2 cut(s) 792, 825
BseNI ACTGG 2 cut(s) 201, 537
BseRI GAGGAG 3 cut(s) 404, 623, 1039
BseSI GKGCMC 1 cut(s) 1101
BseXI GCAGC 3 cut(s) 471, 965, 980
Bsh1236I CGCG 2 cut(s) 672, 922
BshFI GGCC 2 cut(s) 867, 1105
BshNI GGYRCC 1 cut(s) 1148
BshVI ATCGAT 1 cut(s) 572
BsiHKAI GWGCWC 2 cut(s) 218, 582
BsiSI CCGG 3 cut(s) 228, 286, 1146
BslFI GGGAC 3 cut(s) 92, 219, 1121
BslI CCNNNNNNNGG 2 cut(s) 926, 1145
BsmAI GTCTC 1 cut(s) 1021
BsmFI GGGAC 3 cut(s) 92, 219, 1121
BsnI GGCC 2 cut(s) 867, 1105
Bsp1286I GDGCHC 3 cut(s) 218, 582, 1101
Bsp143I GATC 4 cut(s) 634, 698, 789, 838
Bsp1720I GCTNAGC 1 cut(s) 801
BspACI CCGC 2 cut(s) 503, 920
BspANI GGCC 2 cut(s) 867, 1105
BspCNI CTCAG 2 cut(s) 793, 826
BspDI ATCGAT 1 cut(s) 572
BspFNI CGCG 2 cut(s) 672, 922
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 2 cut(s) 207, 1150
BspMI ACCTGC 2 cut(s) 541, 1115
BspPI GGATC 4 cut(s) 629, 693, 784, 846
BspT107I GGYRCC 1 cut(s) 1148
BsrFI RCCGGY 1 cut(s) 1145
BsrI ACTGG 2 cut(s) 201, 537
BssAI RCCGGY 1 cut(s) 1145
BssECI CCNNGG 1 cut(s) 1015
BssMI GATC 4 cut(s) 634, 698, 789, 838
BssSI CACGAG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 544, 1051
Bst6I CTCTTC 3 cut(s) 381, 716, 971
BstAPI GCANNNNNTGC 1 cut(s) 622
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 2 cut(s) 801, 834
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 2 cut(s) 672, 922
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 2 cut(s) 672, 958
BstKTI GATC 4 cut(s) 637, 701, 792, 841
BstMAI GTCTC 1 cut(s) 1021
BstMBI GATC 4 cut(s) 634, 698, 789, 838
BstMWI GCNNNNNNNGC 2 cut(s) 377, 622
BstNSI RCATGY 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 284
BstSLI GKGCMC 1 cut(s) 1101
BstUI CGCG 2 cut(s) 672, 922
BstV1I GCAGC 3 cut(s) 471, 965, 980
BstV2I GAAGAC 2 cut(s) 189, 535
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
Bsu15I ATCGAT 1 cut(s) 572
Bsu36I CCTNAGG 1 cut(s) 834
BsuRI GGCC 2 cut(s) 867, 1105
BsuTUI ATCGAT 1 cut(s) 572
BtgZI GCGATG 1 cut(s) 723
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 625
BtsI GCAGTG 2 cut(s) 422, 431
BtsIMutI CAGTG 3 cut(s) 422, 431, 1056
BveI ACCTGC 2 cut(s) 541, 1115
Cac8I GCNNGC 1 cut(s) 438
CciI TCATGA 1 cut(s) 592
CfoI GCGC 2 cut(s) 672, 958
Cfr10I RCCGGY 1 cut(s) 1145
Cfr13I GGNCC 4 cut(s) 206, 230, 272, 1103
ClaI ATCGAT 1 cut(s) 572
CseI GACGC 1 cut(s) 902
CviAII CATG 4 cut(s) 131, 236, 453, 593
DdeI CTNAG 2 cut(s) 801, 834
DpnI GATC 4 cut(s) 636, 700, 791, 840
DpnII GATC 4 cut(s) 634, 698, 789, 838
DraI TTTAAA 1 cut(s) 294
DraIII CACNNNGTG 1 cut(s) 664
DrdI GACNNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 270
Eam1104I CTCTTC 3 cut(s) 381, 716, 971
EarI CTCTTC 3 cut(s) 381, 716, 971
Ecl136II GAGCTC 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 218
Eco47I GGWCC 3 cut(s) 206, 230, 272
Eco47III AGCGCT 1 cut(s) 957
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 740
Eco81I CCTNAGG 1 cut(s) 834
EcoICRI GAGCTC 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 218
FaeI CATG 4 cut(s) 134, 239, 456, 596
FalI AAGNNNNNCTT 2 cut(s) 942, 974
FaqI GGGAC 3 cut(s) 92, 219, 1121
FatI CATG 4 cut(s) 130, 235, 452, 592
FauI CCCGC 1 cut(s) 496
Fnu4HI GCNGC 3 cut(s) 485, 954, 969
FokI GGATG 1 cut(s) 612
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 3 cut(s) 485, 954, 969
FspBI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
GlaI GCGC 2 cut(s) 671, 957
GluI GCNGC 3 cut(s) 485, 954, 969
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 2 cut(s) 867, 1105
HapII CCGG 3 cut(s) 228, 286, 1146
HgaI GACGC 1 cut(s) 902
HhaI GCGC 2 cut(s) 672, 958
Hin1II CATG 4 cut(s) 134, 239, 456, 596
Hin6I GCGC 2 cut(s) 670, 956
HinP1I GCGC 2 cut(s) 670, 956
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HinfI GANTC 7 cut(s) 119, 200, 224, 337, 509, 596, 1070
HpaII CCGG 3 cut(s) 228, 286, 1146
HphI GGTGA 4 cut(s) 24, 71, 355, 1033
Hpy166II GTNNAC 2 cut(s) 327, 901
Hpy188I TCNGA 5 cut(s) 21, 334, 764, 862, 997
Hpy8I GTNNAC 2 cut(s) 327, 901
Hpy99I CGWCG 1 cut(s) 1043
HpyAV CCTTC 2 cut(s) 670, 787
HpyCH4III ACNGT 2 cut(s) 544, 1051
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 6 cut(s) 452, 587, 616, 625, 857, 935
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 622
HpyF3I CTNAG 2 cut(s) 801, 834
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 4 cut(s) 134, 239, 456, 596
HspAI GCGC 2 cut(s) 670, 956
Kzo9I GATC 4 cut(s) 634, 698, 789, 838
LmnI GCTCC 2 cut(s) 221, 874
Lsp1109I GCAGC 3 cut(s) 471, 965, 980
LweI GCATC 5 cut(s) 136, 634, 700, 866, 1084
MaeI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 30
MalI GATC 4 cut(s) 636, 700, 791, 840
MboI GATC 4 cut(s) 634, 698, 789, 838
MboII GAAGA 5 cut(s) 194, 398, 540, 733, 988
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 3 cut(s) 218, 582, 1101
MluCI AATT 6 cut(s) 64, 93, 159, 172, 252, 731
MmeI TCCRAC 2 cut(s) 159, 229
MseI TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
MslI CAYNNNNRTG 3 cut(s) 135, 630, 1049
MspI CCGG 3 cut(s) 228, 286, 1146
MspR9I CCNGG 1 cut(s) 286
MvnI CGCG 2 cut(s) 672, 922
MwoI GCNNNNNNNGC 2 cut(s) 377, 622
NciI CCSGG 1 cut(s) 286
NdeII GATC 4 cut(s) 634, 698, 789, 838
NlaIII CATG 4 cut(s) 134, 239, 456, 596
NlaIV GGNNCC 2 cut(s) 207, 1150
NmuCI GTSAC 1 cut(s) 30
NspI RCATGY 1 cut(s) 456
PagI TCATGA 1 cut(s) 592
PaqCI CACCTGC 1 cut(s) 1115
PfeI GAWTC 7 cut(s) 119, 200, 224, 337, 509, 596, 1070
PflFI GACNNNGTC 1 cut(s) 905
PkrI GCNGC 3 cut(s) 486, 955, 970
Psp124BI GAGCTC 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 207, 1150
PspPI GGNCC 4 cut(s) 206, 230, 272, 1103
PsrI GAACNNNNNNTAC 2 cut(s) 546, 578
PsuI RGATCY 1 cut(s) 698
PsyI GACNNNGTC 1 cut(s) 905
RseI CAYNNNNRTG 3 cut(s) 135, 630, 1049
SacI GAGCTC 1 cut(s) 218
SaqAI TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
SatI GCNGC 3 cut(s) 485, 954, 969
Sau3AI GATC 4 cut(s) 634, 698, 789, 838
Sau96I GGNCC 4 cut(s) 206, 230, 272, 1103
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 3 cut(s) 218, 582, 1101
SfaNI GCATC 5 cut(s) 136, 634, 700, 866, 1084
SinI GGWCC 3 cut(s) 206, 230, 272
SmiMI CAYNNNNRTG 3 cut(s) 135, 630, 1049
Sse9I AATT 6 cut(s) 64, 93, 159, 172, 252, 731
SsiI CCGC 2 cut(s) 503, 920
SspMI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 284
TaaI ACNGT 2 cut(s) 544, 1051
TaiI ACGT 1 cut(s) 84
TaqI TCGA 5 cut(s) 54, 91, 149, 222, 572
TaqII GACCGA 1 cut(s) 1029
TasI AATT 6 cut(s) 64, 93, 159, 172, 252, 731
TfiI GAWTC 7 cut(s) 119, 200, 224, 337, 509, 596, 1070
Tru1I TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
Tru9I TTAA 9 cut(s) 135, 293, 546, 653, 735, 795, 881, 1001, 1119
TscAI CASTG 3 cut(s) 422, 438, 1056
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 3 cut(s) 484, 953, 968
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 2 cut(s) 282, 1105
TspRI CASTG 3 cut(s) 422, 438, 1056
Tth111I GACNNNGTC 1 cut(s) 905
VpaK11BI GGWCC 3 cut(s) 206, 230, 272
XapI RAATTY 3 cut(s) 64, 252, 731
XceI RCATGY 1 cut(s) 456
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XspI CTAG 5 cut(s) 404, 524, 815, 1067, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.