Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010042

Physical Location & Seq

Genomic Coordinates Reverse (-)

4382198 .. 4384436

Gene Structure 2239 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G13450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGCGGCAGCTCTTGTACGCAGATTCTGCCGGGGATCATCTTTTCCGGTCTCTGGTCATGGATATAGGATGCTATCGACGGTTGAAAATGTATCTGAAAGTGGTAAATCGATGAATCTATACTCTGCTATTAATCAAGCTCTTCACATCGCTTTAGAGACCGATCCTCGGTCTTATGTTTTTGGGGAAGATGTTGGATTTGGTGGTGTCTTCCGATGTACAACAGGATTAGCTGAACGTTTTGGGAAATCTCGAGTTTTCAACACTCCTCTCTGCGAGCAAGGCATTGTTGGCTTTGGCATTGGTTTAGCTGCAATGGGAAATCGAGTTATTGCGGAAATTCAGTTTGCGGATTATATTTTCCCGGCTTTTGATCAGATTGTTAATGAGGCTGCAAAGTTCAGATACCGAAGTGGAAACCAGTTTAACTGTGGAGGACTCACTATTAGGGCACCGTACGGAGCAGTTGGTCACGGTGGACATTACCACTCGCAGTCCCCTGAAGCTTTCTTTTGTCATGTCCCTGGTATCAAAGTTGTTATTCCTCGAAGTCCACGGGAAGCTAAGGGATTATTATTGTCATCCATCCGTGATCCGAATCCCGTTGTCTTTTTCGAACCAAAGTGGCTATATCGTCAAGCGGTAGAGGATGTCCCTGAAGATGACTATATGATACCTTTATCTGAGGCAGAGGTTATGCGAGAAGGTTCCGACATAACTCTTGTTGGATGGGGAGCACAGCTCACTATCATGGAACAAGCATGTCTTGACGCAGAAAACGAAGGAATATCGTGTGAATTGATAGATCTCAAGACTCTGATCCCTTGGGATAAGGAAATCGTTGAGACCTCAGTGAGAAAGACTGGCAGACTTCTTATTAGCCATGAAGCTCCTGTTACAGGAGGCTTTGGGGCCGAGATCGCTGCAACTATAGTTGAGCGTTGCTTCTTGAGGTTAGAAGCACCTGTAAGCAGAGTTTGTGGCCTGGACACTCCTTTCCCTCTAGTGTTCGAACCTTTCTACATGCCAACCAAGAACAAGATATTGGATGCAATCAGATCCACTGTGAATTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006082 GO:0006520 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009267 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009642 GO:0009646 GO:0009743 GO:0009744 GO:0009987 GO:0009991 GO:0010033 GO:0016054 GO:0019752 GO:0030062 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0032991 GO:0033554 GO:0034285 GO:0042221 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043617 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0046395 GO:0050896 GO:0051716 GO:0070013 GO:0071496 GO:0071704 GO:0098798 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00061 ko00280 ko00333 ko00640 ko00780 ko01040 ko01100 ko01110 ko01130 ko01212 map00061 map00280 map00333 map00640 map00780 map01040 map01100 map01110 map01130 map01212

Pfam Domains

Protein Families

Transket_pyr Transketolase_C

Protein Analysis

358

Amino Acids

39.42

Weight (kDa)

5.61

Isoelectric Point (pI)

42.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transket_pyr	PF02779	38 - 212	1.7e-38	Transketolase, pyrimidine binding domain
Transketolase_C	PF02780	229 - 348	9.3e-35	Transketolase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010042)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55510 AT3G13450
fragaria_vesca	FvH4_3g45860 FvH4_3g45860 FvH4_3g45860
malus_domestica	MD03G1002200.v1.1 MD11G1002200.v1.1
prunus_persica	Prupe.6G002000_v2.0.a1 Prupe.6G002000_v2.0.a1
pyrus_communis	pycom03g00210
rosa_chinensis	RchiOBHm_Chr5g0083951
rosa_laevigata	RLG00000037067
rosa_multiflora	Rmu_ssc0000372.1_g000073
rosa_roxburghii	Rroxscaffold_1G00000670
rosa_rugosa	Rorug05G0487800 Rorug05G0487900
rosa_samantha	Rh5AG540700 Rh5BG565700 Rh5CG587600 Rh5DG574300
rosa_wichuraiana	Rw5G050280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	451
AciI	CCGC	4 cut(s)	5, 335, 350, 641
AclI	AACGTT	1 cut(s)	238
AclWI	GGATC	5 cut(s)	43, 158, 587, 814, 1053
AcsI	RAATTY	1 cut(s)	339
AcuI	CTGAAG	2 cut(s)	522, 678
AfaI	GTAC	3 cut(s)	18, 220, 458
AfiI	CCNNNNNNNGG	3 cut(s)	53, 168, 899
AgsI	TTSAA	2 cut(s)	86, 262
AjnI	CCWGG	2 cut(s)	523, 984
AjuI	GAANNNNNNNTTGG	2 cut(s)	1028, 1060
AluBI	AGCT	8 cut(s)	11, 140, 233, 311, 506, 563, 742, 890
AluI	AGCT	8 cut(s)	11, 140, 233, 311, 506, 563, 742, 890
Alw21I	GWGCWC	1 cut(s)	739
Alw26I	GTCTC	3 cut(s)	55, 152, 839
AlwI	GGATC	5 cut(s)	43, 158, 587, 814, 1053
AlwNI	CAGNNNCTG	1 cut(s)	27
Ama87I	CYCGRG	1 cut(s)	252
AoxI	GGCC	2 cut(s)	912, 982
ApeKI	GCWGC	4 cut(s)	8, 311, 392, 923
ApoI	RAATTY	1 cut(s)	339
AseI	ATTAAT	1 cut(s)	132
AspS9I	GGNCC	1 cut(s)	912
AsuC2I	CCSGG	2 cut(s)	32, 365
AsuII	TTCGAA	2 cut(s)	615, 1011
AvaI	CYCGRG	1 cut(s)	252
BaeGI	GKGCMC	1 cut(s)	454
BaeI	ACNNNNGTAYC	2 cut(s)	397, 430
BanI	GGYRCC	1 cut(s)	451
BbsI	GAAGAC	1 cut(s)	202
Bbv12I	GWGCWC	1 cut(s)	739
BbvI	GCAGC	4 cut(s)	20, 298, 379, 910
BccI	CCATC	2 cut(s)	593, 723
BcgI	CGANNNNNNTGC	2 cut(s)	905, 939
BciT130I	CCWGG	2 cut(s)	525, 986
BclI	TGATCA	1 cut(s)	373
BcnI	CCSGG	2 cut(s)	32, 365
BcoDI	GTCTC	3 cut(s)	55, 152, 839
BfaI	CTAG	2 cut(s)	1004, 1075
BfmI	CTRYAG	1 cut(s)	930
BglII	AGATCT	1 cut(s)	805
BisI	GCNGC	5 cut(s)	6, 9, 312, 393, 924
BlsI	GCNGC	5 cut(s)	7, 10, 313, 394, 925
Bme1390I	CCNGG	4 cut(s)	32, 365, 525, 986
BmeT110I	CYCGRG	1 cut(s)	252
BmgT120I	GGNCC	1 cut(s)	912
BmiI	GGNNCC	3 cut(s)	453, 709, 913
BmrFI	CCNGG	4 cut(s)	32, 365, 525, 986
BmsI	GCATC	2 cut(s)	60, 1039
BpiI	GAAGAC	1 cut(s)	202
BplI	GAGNNNNNCTC	2 cut(s)	726, 758
Bpu10I	CCTNAGC	1 cut(s)	564
Bpu14I	TTCGAA	2 cut(s)	615, 1011
BpuEI	CTTGAG	2 cut(s)	794, 970
BpuMI	CCSGG	2 cut(s)	32, 365
Bsa29I	ATCGAT	1 cut(s)	110
BsaBI	GATNNNNATC	1 cut(s)	597
BsaI	GGTCTC	3 cut(s)	55, 152, 839
BsaJI	CCNNGG	5 cut(s)	31, 167, 523, 554, 824
BsaWI	WCCGGW	1 cut(s)	46
Bsc4I	CCNNNNNNNGG	3 cut(s)	53, 168, 899
Bse1I	ACTGG	2 cut(s)	421, 868
Bse3DI	GCAATG	1 cut(s)	321
Bse8I	GATNNNNATC	1 cut(s)	597
BseBI	CCWGG	2 cut(s)	525, 986
BseCI	ATCGAT	1 cut(s)	110
BseDI	CCNNGG	5 cut(s)	31, 167, 523, 554, 824
BseGI	GGATG	6 cut(s)	75, 581, 585, 655, 734, 1054
BseJI	GATNNNNATC	1 cut(s)	597
BseLI	CCNNNNNNNGG	3 cut(s)	53, 168, 899
BseMI	GCAATG	1 cut(s)	321
BseMII	CTCAG	2 cut(s)	675, 864
BseNI	ACTGG	2 cut(s)	421, 868
BseRI	GAGGAG	1 cut(s)	258
BseSI	GKGCMC	1 cut(s)	454
BseXI	GCAGC	4 cut(s)	20, 298, 379, 910
BshFI	GGCC	2 cut(s)	914, 984
BshNI	GGYRCC	1 cut(s)	451
BshVI	ATCGAT	1 cut(s)	110
BsiHKAI	GWGCWC	1 cut(s)	739
BsiHKCI	CYCGRG	1 cut(s)	252
BsiSI	CCGG	3 cut(s)	31, 47, 365
BsiWI	CGTACG	1 cut(s)	456
BslFI	GGGAC	3 cut(s)	481, 506, 638
BslI	CCNNNNNNNGG	3 cut(s)	53, 168, 899
BsmAI	GTCTC	3 cut(s)	55, 152, 839
BsmFI	GGGAC	3 cut(s)	481, 506, 638
BsnI	GGCC	2 cut(s)	914, 984
Bso31I	GGTCTC	3 cut(s)	55, 152, 839
BsoBI	CYCGRG	1 cut(s)	252
Bsp119I	TTCGAA	2 cut(s)	615, 1011
Bsp1286I	GDGCHC	2 cut(s)	454, 739
Bsp1407I	TGTACA	1 cut(s)	218
Bsp143I	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
BspACI	CCGC	4 cut(s)	5, 335, 350, 641
BspANI	GGCC	2 cut(s)	914, 984
BspCNI	CTCAG	2 cut(s)	676, 863
BspDI	ATCGAT	1 cut(s)	110
BspLI	GGNNCC	3 cut(s)	453, 709, 913
BspPI	GGATC	5 cut(s)	43, 158, 587, 814, 1053
BspQI	GCTCTTC	1 cut(s)	147
BspT104I	TTCGAA	2 cut(s)	615, 1011
BspT107I	GGYRCC	1 cut(s)	451
BspTNI	GGTCTC	3 cut(s)	55, 152, 839
BsrDI	GCAATG	1 cut(s)	321
BsrGI	TGTACA	1 cut(s)	218
BsrI	ACTGG	2 cut(s)	421, 868
BssECI	CCNNGG	5 cut(s)	31, 167, 523, 554, 824
BssMI	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
BssT1I	CCWWGG	1 cut(s)	824
Bst2UI	CCWGG	2 cut(s)	525, 986
Bst4CI	ACNGT	5 cut(s)	82, 431, 456, 476, 1066
Bst6I	CTCTTC	1 cut(s)	147
BstAPI	GCANNNNNTGC	1 cut(s)	27
BstAUI	TGTACA	1 cut(s)	218
BstBI	TTCGAA	2 cut(s)	615, 1011
BstC8I	GCNNGC	1 cut(s)	278
BstDEI	CTNAG	3 cut(s)	564, 684, 850
BstDSI	CCRYGG	1 cut(s)	554
BstENI	CCTNNNNNAGG	1 cut(s)	897
BstF5I	GGATG	6 cut(s)	75, 581, 585, 655, 734, 1054
BstKTI	GATC	8 cut(s)	38, 166, 376, 595, 808, 822, 921, 1061
BstMAI	GTCTC	3 cut(s)	55, 152, 839
BstMBI	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
BstMWI	GCNNNNNNNGC	4 cut(s)	27, 282, 291, 920
BstNI	CCWGG	2 cut(s)	525, 986
BstNSI	RCATGY	2 cut(s)	765, 1027
BstSCI	CCNGG	4 cut(s)	30, 363, 523, 984
BstSFI	CTRYAG	1 cut(s)	930
BstSLI	GKGCMC	1 cut(s)	454
BstV1I	GCAGC	4 cut(s)	20, 298, 379, 910
BstV2I	GAAGAC	1 cut(s)	202
BstX2I	RGATCY	2 cut(s)	805, 1058
BstYI	RGATCY	2 cut(s)	805, 1058
Bsu15I	ATCGAT	1 cut(s)	110
BsuRI	GGCC	2 cut(s)	914, 984
BsuTUI	ATCGAT	1 cut(s)	110
BtgI	CCRYGG	1 cut(s)	554
BtgZI	GCGATG	1 cut(s)	133
BtsCI	GGATG	6 cut(s)	75, 581, 585, 655, 734, 1054
BtsIMutI	CAGTG	2 cut(s)	858, 1062
Cac8I	GCNNGC	1 cut(s)	278
CaiI	CAGNNNCTG	1 cut(s)	27
Cfr13I	GGNCC	1 cut(s)	912
ClaI	ATCGAT	1 cut(s)	110
CseI	GACGC	1 cut(s)	779
Csp6I	GTAC	3 cut(s)	17, 219, 457
CviAII	CATG	6 cut(s)	59, 518, 751, 762, 884, 1024
CviQI	GTAC	3 cut(s)	17, 219, 457
DdeI	CTNAG	3 cut(s)	564, 684, 850
DpnI	GATC	8 cut(s)	37, 165, 375, 594, 807, 821, 920, 1060
DpnII	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
Eam1104I	CTCTTC	1 cut(s)	147
EarI	CTCTTC	1 cut(s)	147
Eco130I	CCWWGG	1 cut(s)	824
Eco31I	GGTCTC	3 cut(s)	55, 152, 839
Eco57I	CTGAAG	2 cut(s)	522, 678
Eco88I	CYCGRG	1 cut(s)	252
EcoNI	CCTNNNNNAGG	1 cut(s)	897
EcoRII	CCWGG	2 cut(s)	523, 984
EcoT14I	CCWWGG	1 cut(s)	824
ErhI	CCWWGG	1 cut(s)	824
FaeI	CATG	6 cut(s)	62, 521, 754, 765, 887, 1027
FalI	AAGNNNNNCTT	2 cut(s)	750, 782
FaqI	GGGAC	3 cut(s)	481, 506, 638
FatI	CATG	6 cut(s)	58, 517, 750, 761, 883, 1023
FbaI	TGATCA	1 cut(s)	373
Fnu4HI	GCNGC	5 cut(s)	6, 9, 312, 393, 924
FokI	GGATG	6 cut(s)	82, 568, 572, 662, 741, 1061
Fsp4HI	GCNGC	5 cut(s)	6, 9, 312, 393, 924
FspBI	CTAG	2 cut(s)	1004, 1075
GluI	GCNGC	5 cut(s)	6, 9, 312, 393, 924
HaeIII	GGCC	2 cut(s)	914, 984
HapII	CCGG	3 cut(s)	31, 47, 365
HgaI	GACGC	1 cut(s)	779
Hin1II	CATG	6 cut(s)	62, 521, 754, 765, 887, 1027
HindIII	AAGCTT	1 cut(s)	504
HinfI	GANTC	5 cut(s)	24, 115, 438, 598, 814
HpaII	CCGG	3 cut(s)	31, 47, 365
Hpy166II	GTNNAC	2 cut(s)	479, 554
Hpy188I	TCNGA	9 cut(s)	97, 215, 378, 404, 597, 685, 712, 819, 1058
Hpy188III	TCNNGA	4 cut(s)	252, 767, 811, 949
Hpy8I	GTNNAC	2 cut(s)	479, 554
Hpy99I	CGWCG	1 cut(s)	82
HpyAV	CCTTC	2 cut(s)	698, 776
HpyCH4III	ACNGT	5 cut(s)	82, 431, 456, 476, 1066
HpyCH4IV	ACGT	1 cut(s)	238
HpyCH4V	TGCA	4 cut(s)	314, 395, 926, 1052
HpyF10VI	GCNNNNNNNGC	4 cut(s)	27, 282, 291, 920
HpyF3I	CTNAG	3 cut(s)	564, 684, 850
HpySE526I	ACGT	1 cut(s)	238
Hsp92II	CATG	6 cut(s)	62, 521, 754, 765, 887, 1027
Ksp22I	TGATCA	1 cut(s)	373
Kzo9I	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
LguI	GCTCTTC	1 cut(s)	147
LmnI	GCTCC	3 cut(s)	461, 734, 895
Lsp1109I	GCAGC	4 cut(s)	20, 298, 379, 910
LweI	GCATC	2 cut(s)	60, 1039
MaeI	CTAG	2 cut(s)	1004, 1075
MaeII	ACGT	1 cut(s)	238
MaeIII	GTNAC	2 cut(s)	470, 895
MalI	GATC	8 cut(s)	37, 165, 375, 594, 807, 821, 920, 1060
MboI	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
MboII	GAAGA	4 cut(s)	134, 200, 202, 671
MflI	RGATCY	2 cut(s)	805, 1058
MhlI	GDGCHC	2 cut(s)	454, 739
MluCI	AATT	3 cut(s)	339, 797, 1069
MlyI	GAGTC	2 cut(s)	432, 808
MmeI	TCCRAC	3 cut(s)	175, 706, 735
MseI	TTAA	3 cut(s)	132, 384, 426
MslI	CAYNNNNRTG	1 cut(s)	749
MspI	CCGG	3 cut(s)	31, 47, 365
MspR9I	CCNGG	4 cut(s)	32, 365, 525, 986
MvaI	CCWGG	2 cut(s)	525, 986
MwoI	GCNNNNNNNGC	4 cut(s)	27, 282, 291, 920
NciI	CCSGG	2 cut(s)	32, 365
NdeII	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
NlaIII	CATG	6 cut(s)	62, 521, 754, 765, 887, 1027
NlaIV	GGNNCC	3 cut(s)	453, 709, 913
NmeAIII	GCCGAG	1 cut(s)	940
NmuCI	GTSAC	1 cut(s)	470
NspI	RCATGY	2 cut(s)	765, 1027
NspV	TTCGAA	2 cut(s)	615, 1011
PaeR7I	CTCGAG	1 cut(s)	252
PciSI	GCTCTTC	1 cut(s)	147
PcsI	WCGNNNNNNNCGW	1 cut(s)	777
PfeI	GAWTC	3 cut(s)	24, 115, 598
Pfl23II	CGTACG	1 cut(s)	456
PkrI	GCNGC	5 cut(s)	7, 10, 313, 394, 925
PleI	GAGTC	2 cut(s)	432, 808
PpsI	GAGTC	2 cut(s)	432, 808
PshBI	ATTAAT	1 cut(s)	132
Psp1406I	AACGTT	1 cut(s)	238
Psp6I	CCWGG	2 cut(s)	523, 984
PspGI	CCWGG	2 cut(s)	523, 984
PspLI	CGTACG	1 cut(s)	456
PspN4I	GGNNCC	3 cut(s)	453, 709, 913
PspPI	GGNCC	1 cut(s)	912
PstNI	CAGNNNCTG	1 cut(s)	27
PsuI	RGATCY	2 cut(s)	805, 1058
RsaI	GTAC	3 cut(s)	18, 220, 458
RsaNI	GTAC	3 cut(s)	17, 219, 457
RseI	CAYNNNNRTG	1 cut(s)	749
SapI	GCTCTTC	1 cut(s)	147
SaqAI	TTAA	3 cut(s)	132, 384, 426
SatI	GCNGC	5 cut(s)	6, 9, 312, 393, 924
Sau3AI	GATC	8 cut(s)	35, 163, 373, 592, 805, 819, 918, 1058
Sau96I	GGNCC	1 cut(s)	912
SchI	GAGTC	2 cut(s)	432, 808
ScrFI	CCNGG	4 cut(s)	32, 365, 525, 986
SduI	GDGCHC	2 cut(s)	454, 739
SfaNI	GCATC	2 cut(s)	60, 1039
SfcI	CTRYAG	1 cut(s)	930
Sfr274I	CTCGAG	1 cut(s)	252
SfuI	TTCGAA	2 cut(s)	615, 1011
SlaI	CTCGAG	1 cut(s)	252
SmiMI	CAYNNNNRTG	1 cut(s)	749
SmlI	CTYRAG	3 cut(s)	252, 809, 949
SmoI	CTYRAG	3 cut(s)	252, 809, 949
Sse9I	AATT	3 cut(s)	339, 797, 1069
SsiI	CCGC	4 cut(s)	5, 335, 350, 641
SspMI	CTAG	2 cut(s)	1004, 1075
StyD4I	CCNGG	4 cut(s)	30, 363, 523, 984
StyI	CCWWGG	1 cut(s)	824
TaaI	ACNGT	5 cut(s)	82, 431, 456, 476, 1066
TaiI	ACGT	1 cut(s)	241
TaqI	TCGA	7 cut(s)	77, 110, 253, 325, 547, 615, 1011
TaqII	GACCGA	2 cut(s)	159, 176
TasI	AATT	3 cut(s)	339, 797, 1069
TatI	WGTACW	1 cut(s)	218
TauI	GCSGC	1 cut(s)	8
TfiI	GAWTC	3 cut(s)	24, 115, 598
Tru1I	TTAA	3 cut(s)	132, 384, 426
Tru9I	TTAA	3 cut(s)	132, 384, 426
TscAI	CASTG	2 cut(s)	858, 1069
TseFI	GTSAC	1 cut(s)	470
TseI	GCWGC	4 cut(s)	8, 311, 392, 923
Tsp45I	GTSAC	1 cut(s)	470
TspDTI	ATGAA	2 cut(s)	128, 900
TspGWI	ACGGA	2 cut(s)	474, 578
TspRI	CASTG	2 cut(s)	858, 1069
VspI	ATTAAT	1 cut(s)	132
XagI	CCTNNNNNAGG	1 cut(s)	897
XapI	RAATTY	1 cut(s)	339
XceI	RCATGY	2 cut(s)	765, 1027
XcmI	CCANNNNNNNNNTGG	1 cut(s)	428
XhoI	CTCGAG	1 cut(s)	252
XspI	CTAG	2 cut(s)	1004, 1075

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.