Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Reverse (-)

6625377 .. 6632164

Gene Structure 6788 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G19170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3243 bp

ATGCTCCGAACCGTCTCTTGTTTAGCTTCGCGCAGCTCGTCCTCTCTATTCTTTCGCTTCTTTCGTCAGTTCCCTCGCTCTTACATGTCTCTCACATCGTCGACGGCAGCTCTCCGTGTCCCCAGTCGCAATCTTCGCCGAATTTCCTCTCCCTCAGTCGCCGGTAGACGTCTTTTGCTCCGACGAGGTTTGAGGATTCCCTCCGCCGCAGTTCGCAGTGTCAATGGGCAGTTCTCTCGCCTCTCTGTTCGCGCCGTCGCCACACAACCCGCTCCACTTTATCCTGATGTAGGTCAAGATGAGGCTGAGAAGCTTGGATTTGAAAAAGTGTCGGAAGAATTCATCTCAGAGTGTAAATCAAAGGCGATACTCTTCAAACACAAGAAAACTGGTTGCGAGGTGATGTCTGTGTCAAATGAGGATGAGAACAAGGTCTTCGGTGTTGTTTTCAGAACTCCTCCGAAGGATTCCACTGGCATTCCGCACATACTGGAACATAGCGTTCTATGTGGGTCAAGAAAGTACCCGGTAAAAGAGCCATTTGTTGAACTACTCAAGGGAAGTTTGCATACTTTTCTAAATGCATTCACATATCCTGATAGGACCTGCTACCCAGTTGCTTCCACCAATACAAAGGATTTTTACAATCTTGTCGATGTATATTTGGATGCTGTCTTCTTCCCCAAGTGTGTGGATGACGCTCACACCTTTCAGCAAGAGGGCTGGCACTATGAACTTAATGATCCCTCGGAAGACATATCTTACAAAGGTGTTGTTTTTAATGAGATGAAAGGGGTCTATTCACAGCCTGATAATATTTTAGGGCGAATTGCTCAACAGGCCTTATCCCCAGAAAATACATATGGTGTTGACAGTGGAGGTGATCCAAAAGATATTCCTAATCTGACATTTGAGGAATTCAAGGAGTTCCACCGTCAATATTATCACCCAAGCAACGCCAGGATCTGGTTCTATGGAGATGATGATCCCGTTCACCGCCTCCGTGTCTTGAGTGAATACTTGGATATGTTTGAAGCAAGTCCATCTCCCAATAGTTCAAAGATTAAATTTCAAAAGCTATTCTCTGAGCCTGTCAGACTGGTTGAGAAATATCCTGCTGGTCGAGATGGTGACCTTAAAAAGAAGCATATGTTGTGCGTTAACTGGCTATTGTCTGAGAAGCCCTTGGACTTGCAAACTCAACTTGCACTTGGCTTCTTGGATCATCTTATGCTGGGGACTCCTGCTTCACCACTAAGAAAGATCTTATTGGAAAGCGGTTTAGGAGAAGCTCTTGTCAGTAGTGGGTTGTCAGACGAACTCTTGCAGCCCCAGTTCGGTATTGGTTTGAAAGGTGTGTCCGAAGAGAATGTTCAAAAGGTTGAAGAGTTGATCATGGATACTCTGAAAAAGTTGGCAGAAGAAGGGTTTGACAATGATGCTGTGGAGGCATCCATGAATACAATTGAGTTTTCTTTGAGGGAAAACAACACAGGATCTTTCCCTCGTGGTTTATCACTTATGCTCCAATCTATTTCAAAATGGATATACGATATGGATCCTTTTGAGCCATTAAAGTATACGGAACCATTGAAGGCCCTGAAAACCAGAATAGCTGAGGAGGGATCCAAGGCTGTCTTTTCCCCACTGATAGAGAAGCTTATCTTGAACAACTCGCATCGTGTTACCATAGAGATGCAGCCTGATCCTGAAAAAGCTACTCAAGAGGAAGTGGAAGAGAAAAATATCCTGGAAAAAGTTAAAGCAGCTATGACAGAAGAAGATCTTGCAGAGCTAGCACGTGCTACAGAGGAGCTGAAATTGAAACAAGAAACTCCTGACCCTCCTGAAGCATTGAGATGTGTCCCAAGTTTGAACTTGGGTGACATTCCAAAAGAACCTACTTATGTTCCCACCGAGGTTGGAGATATTAATGGTGTGAAGGTTTTGCGGCATGACCTTTTCACCAATGATATTATCTATACTGAAGTAGTCTTTGATATTGGTTCGCTGAAACATGAGCTTCTTCCACTAGTACCACTTTTCTGTCAATCATTACTGGAGATGGGCACAAAAGATTTGACTTTTGTTCAACTTAATCAGTTGATTGGAAGGAAAACGGGAGGGATATCAGTTTATCCCCTTACATCATCAGTGAGGGGTAAGGATGAACCATGCAGCAAAATTATCGTACGCGGAAAATCCATGGCTGGGCGTGCTGACGACCTTTTTAACCTGATGAACTGCTTGTTGCAAGAGGTTCAATTTACAGATCAGCAGAGGTTTAAACAGTTTGTCTCTCAAAGCAGAGCACGGATGGAGAACCGATTGAGGGGAAGTGGTCATGGAATTGCTGCGGCGAGGATGGATGCAATGTTGAACATTGCTGGATGGATGTCTGAACAGATGGGTGGTCTCAGCTATCTTGAATTTTTACATACTCTTGAAAAGAAAGTGGATGAAGACTGGGAAGGAATTTCATCCTCGCTTGAGGAGATCAGAAGATCTCTCCTTGCTAGAAACGGTTGCATAGTTAATATGACTGCTGATGGGAAGTCTCTCACAAATGTCGAAAAATCTGTTGCAAAATTTCTAGATTTACTCCCTGAGAACCCGTCTGGTGGGCTTGTCACTTGGGATGGTCGACTTCCTCTGAGAAATGAAGCCATTGTAATACCAACCCAGGTAAACTATGTTGGAAAAGCGGGTAACATATACAGCACCGGGTATGAGCTTGATGGCAGTGCGTATGTTATATCAAAGCATATTAGCAATACATGGTTATGGGATCGTGTTCGTGTAAGTGGTGGTGCATATGGAGGTTTCTGTGATTTCGATTCGCATTCAGGAGTATTTTCTTACTTATCGTACCGGGATCCAAACTTGTTGAAGACACTTGATATATATGATGGAACTGGAGATTTCTTACGTGGCCTTGATGTGGATCAAGAAACACTCACTAAAGCTATTATTGGCACCATCGGTGATGTTGATTCATACCAATTACCTGATGCCAAAGGTTATAGCAGTTTGTTGAGGCATTTACTCGGAGTAACTGACGAAGAACGACAAAGAAAGCGTGAAGAGATACTAACAACAAGTTTGAAGGACTTCAAGGATTTCGCACAAGCAATAGATGTGGTTAGAGATAAAGGTGTTGCGGTGGCTGTGGCATCCGCGGAAGACATTGATGCAGCGAACAATGAACGCTCCAACTTTTTCGAGGTGAAGAAAGCTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

1080

Amino Acids

121.01

Weight (kDa)

5.48

Isoelectric Point (pI)

45.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	154 - 248	1e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	301 - 487	9.1e-29	Peptidase M16 inactive domain
M16C_assoc	PF08367	566 - 815	3.4e-77	Peptidase M16C associated
PreP_C	PF22516	887 - 1059	3.3e-79	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	172
Acc36I	ACCTGC	1 cut(s)	614
AccB1I	GGYRCC	1 cut(s)	2975
AccB7I	CCANNNNNTGG	1 cut(s)	966
AccBSI	CCGCTC	1 cut(s)	272
AccI	GTMKAC	4 cut(s)	101, 166, 1580, 2644
AccII	CGCG	4 cut(s)	31, 252, 2196, 3179
AcsI	RAATTY	7 cut(s)	141, 338, 917, 1067, 2429, 2475, 2588
AcuI	CTGAAG	2 cut(s)	1869, 2007
AcvI	CACGTG	1 cut(s)	1802
AcyI	GRCGYC	1 cut(s)	169
AfaI	GTAC	4 cut(s)	524, 2037, 2193, 2870
AfiI	CCNNNNNNNGG	7 cut(s)	290, 966, 1337, 2211, 2332, 2621, 2941
AflIII	ACRYGT	1 cut(s)	84
AhlI	ACTAGT	1 cut(s)	2032
AjnI	CCWGG	3 cut(s)	959, 1749, 2682
Alw21I	GWGCWC	1 cut(s)	2314
Alw26I	GTCTC	5 cut(s)	19, 93, 2302, 2420, 2562
AlwNI	CAGNNNCTG	1 cut(s)	966
AoxI	GGCC	3 cut(s)	840, 1596, 2932
ApeKI	GCWGC	8 cut(s)	33, 107, 1327, 1699, 1766, 2178, 2354, 3194
ApoI	RAATTY	7 cut(s)	141, 338, 917, 1067, 2429, 2475, 2588
AseI	ATTAAT	1 cut(s)	1932
Asp700I	GAANNNNTTC	1 cut(s)	3110
AspLEI	GCGC	2 cut(s)	33, 254
AspS9I	GGNCC	2 cut(s)	603, 1597
AsuC2I	CCSGG	3 cut(s)	527, 2725, 2873
AsuNHI	GCTAGC	1 cut(s)	1795
AvaII	GGWCC	1 cut(s)	603
BaeGI	GKGCMC	1 cut(s)	2072
BamHI	GGATCC	3 cut(s)	1558, 1625, 2875
BanI	GGYRCC	1 cut(s)	2975
BarI	GAAGNNNNNNTAC	2 cut(s)	553, 585
BauI	CACGAG	1 cut(s)	1506
BbrPI	CACGTG	1 cut(s)	1802
BbsI	GAAGAC	6 cut(s)	427, 667, 759, 2469, 2897, 3189
Bbv12I	GWGCWC	1 cut(s)	2314
BbvCI	CCTCAGC	1 cut(s)	1617
BbvI	GCAGC	8 cut(s)	45, 119, 1339, 1711, 1778, 2190, 2341, 3206
BceAI	ACGGC	2 cut(s)	120, 239
BcgI	CGANNNNNNTGC	4 cut(s)	218, 252, 2170, 2204
BciT130I	CCWGG	3 cut(s)	961, 1751, 2684
BciVI	GTATCC	1 cut(s)	1393
BclI	TGATCA	1 cut(s)	1392
BcnI	CCSGG	3 cut(s)	527, 2725, 2873
BcoDI	GTCTC	5 cut(s)	19, 93, 2302, 2420, 2562
BcuI	ACTAGT	1 cut(s)	2032
BfaI	CTAG	4 cut(s)	1796, 2033, 2517, 2594
BfmI	CTRYAG	1 cut(s)	1806
BfuAI	ACCTGC	1 cut(s)	614
BfuI	GTATCC	1 cut(s)	1393
BglII	AGATCT	3 cut(s)	1263, 1783, 2504
Bme1390I	CCNGG	6 cut(s)	527, 961, 1751, 2684, 2725, 2873
Bme18I	GGWCC	1 cut(s)	603
BmgT120I	GGNCC	2 cut(s)	603, 1597
BmiI	GGNNCC	5 cut(s)	1560, 1587, 1627, 2877, 2977
BmrFI	CCNGG	6 cut(s)	527, 961, 1751, 2684, 2725, 2873
BmrI	ACTGGG	4 cut(s)	117, 608, 1327, 2476
BmsI	GCATC	9 cut(s)	658, 1429, 1460, 1686, 1687, 2359, 3001, 3181, 3182
BmtI	GCTAGC	1 cut(s)	1799
BmuI	ACTGGG	4 cut(s)	117, 608, 1327, 2476
BpiI	GAAGAC	6 cut(s)	427, 667, 759, 2469, 2897, 3189
BpmI	CTGGAG	2 cut(s)	2081, 2937
Bpu10I	CCTNAGC	1 cut(s)	1617
BpuEI	CTTGAG	4 cut(s)	539, 1030, 1707, 2510
BpuMI	CCSGG	3 cut(s)	527, 2725, 2873
BsaAI	YACGTR	2 cut(s)	1802, 2930
BsaBI	GATNNNNATC	3 cut(s)	984, 1557, 2943
BsaHI	GRCGYC	1 cut(s)	169
BsaI	GGTCTC	1 cut(s)	2420
BsaJI	CCNNGG	7 cut(s)	747, 1185, 1629, 1917, 2205, 2682, 3177
BsaXI	ACNNNNNCTCC	2 cut(s)	1509, 1539
Bsc4I	CCNNNNNNNGG	7 cut(s)	290, 966, 1337, 2211, 2332, 2621, 2941
Bse118I	RCCGGY	1 cut(s)	161
Bse3DI	GCAATG	2 cut(s)	2379, 2382
Bse8I	GATNNNNATC	3 cut(s)	984, 1557, 2943
BseBI	CCWGG	3 cut(s)	961, 1751, 2684
BseDI	CCNNGG	7 cut(s)	747, 1185, 1629, 1917, 2205, 2682, 3177
BseJI	GATNNNNATC	3 cut(s)	984, 1557, 2943
BseLI	CCNNNNNNNGG	7 cut(s)	290, 966, 1337, 2211, 2332, 2621, 2941
BseMI	GCAATG	2 cut(s)	2379, 2382
BseMII	CTCAG	9 cut(s)	168, 297, 360, 1077, 1167, 1608, 2431, 2598, 2645
BseRI	GAGGAG	4 cut(s)	447, 1634, 1826, 2507
BseSI	GKGCMC	1 cut(s)	2072
BseXI	GCAGC	8 cut(s)	45, 119, 1339, 1711, 1778, 2190, 2341, 3206
BseYI	CCCAGC	2 cut(s)	1234, 2210
Bsh1236I	CGCG	4 cut(s)	31, 252, 2196, 3179
BshFI	GGCC	3 cut(s)	842, 1598, 2934
BshNI	GGYRCC	1 cut(s)	2975
BsiHKAI	GWGCWC	1 cut(s)	2314
BsiSI	CCGG	4 cut(s)	162, 527, 2724, 2872
BsiWI	CGTACG	1 cut(s)	2191
BslFI	GGGAC	3 cut(s)	104, 1252, 1850
BslI	CCNNNNNNNGG	7 cut(s)	290, 966, 1337, 2211, 2332, 2621, 2941
BsmAI	GTCTC	5 cut(s)	19, 93, 2302, 2420, 2562
BsmBI	CGTCTC	1 cut(s)	19
BsmFI	GGGAC	3 cut(s)	104, 1252, 1850
BsmI	GAATGC	3 cut(s)	477, 584, 2842
BsnI	GGCC	3 cut(s)	842, 1598, 2934
Bso31I	GGTCTC	1 cut(s)	2420
Bsp1286I	GDGCHC	2 cut(s)	2072, 2314
Bsp19I	CCATGG	1 cut(s)	2205
BspANI	GGCC	3 cut(s)	842, 1598, 2934
BspCNI	CTCAG	9 cut(s)	167, 298, 359, 1078, 1168, 1609, 2430, 2599, 2646
BspFNI	CGCG	4 cut(s)	31, 252, 2196, 3179
BspLI	GGNNCC	5 cut(s)	1560, 1587, 1627, 2877, 2977
BspMI	ACCTGC	1 cut(s)	614
BspOI	GCTAGC	1 cut(s)	1799
BspT107I	GGYRCC	1 cut(s)	2975
BspTNI	GGTCTC	1 cut(s)	2420
BsrBI	CCGCTC	1 cut(s)	272
BsrDI	GCAATG	2 cut(s)	2379, 2382
BsrFI	RCCGGY	1 cut(s)	161
BssAI	RCCGGY	1 cut(s)	161
BssECI	CCNNGG	7 cut(s)	747, 1185, 1629, 1917, 2205, 2682, 3177
BssNAI	GTATAC	1 cut(s)	1581
BssNI	GRCGYC	1 cut(s)	169
BssSI	CACGAG	1 cut(s)	1506
BssT1I	CCWWGG	3 cut(s)	1185, 1629, 2205
Bst1107I	GTATAC	1 cut(s)	1581
Bst2BI	CACGAG	1 cut(s)	1506
Bst2UI	CCWGG	3 cut(s)	961, 1751, 2684
Bst4CI	ACNGT	5 cut(s)	13, 875, 935, 2292, 2525
Bst6I	CTCTTC	5 cut(s)	377, 1359, 1380, 1731, 3078
BstACI	GRCGYC	1 cut(s)	169
BstBAI	YACGTR	2 cut(s)	1802, 2930
BstC8I	GCNNGC	3 cut(s)	725, 1797, 2217
BstDSI	CCRYGG	2 cut(s)	2205, 3177
BstEII	GGTNACC	1 cut(s)	1130
BstENI	CCTNNNNNAGG	1 cut(s)	288
BstFNI	CGCG	4 cut(s)	31, 252, 2196, 3179
BstHHI	GCGC	2 cut(s)	33, 254
BstMAI	GTCTC	5 cut(s)	19, 93, 2302, 2420, 2562
BstMWI	GCNNNNNNNGC	5 cut(s)	135, 839, 1448, 1796, 2216
BstNI	CCWGG	3 cut(s)	961, 1751, 2684
BstNSI	RCATGY	1 cut(s)	88
BstPI	GGTNACC	1 cut(s)	1130
BstSCI	CCNGG	6 cut(s)	525, 959, 1749, 2682, 2723, 2871
BstSFI	CTRYAG	1 cut(s)	1806
BstSLI	GKGCMC	1 cut(s)	2072
BstUI	CGCG	4 cut(s)	31, 252, 2196, 3179
BstV1I	GCAGC	8 cut(s)	45, 119, 1339, 1711, 1778, 2190, 2341, 3206
BstV2I	GAAGAC	6 cut(s)	427, 667, 759, 2469, 2897, 3189
BstX2I	RGATCY	8 cut(s)	963, 1263, 1496, 1558, 1625, 1783, 2504, 2875
BstXI	CCANNNNNNTGG	1 cut(s)	691
BstYI	RGATCY	8 cut(s)	963, 1263, 1496, 1558, 1625, 1783, 2504, 2875
BstZ17I	GTATAC	1 cut(s)	1581
BsuI	GTATCC	1 cut(s)	1393
BsuRI	GGCC	3 cut(s)	842, 1598, 2934
BtgI	CCRYGG	2 cut(s)	2205, 3177
BtsI	GCAGTG	2 cut(s)	223, 2749
BtsIMutI	CAGTG	6 cut(s)	223, 471, 880, 1646, 2160, 2749
BveI	ACCTGC	1 cut(s)	614
Cac8I	GCNNGC	3 cut(s)	725, 1797, 2217
CaiI	CAGNNNCTG	1 cut(s)	966
CfoI	GCGC	2 cut(s)	33, 254
Cfr10I	RCCGGY	1 cut(s)	161
Cfr13I	GGNCC	2 cut(s)	603, 1597
Cfr42I	CCGCGG	1 cut(s)	3180
CseI	GACGC	1 cut(s)	707
Csp6I	GTAC	4 cut(s)	523, 2036, 2192, 2869
CspCI	CAANNNNNGTGG	4 cut(s)	1903, 1938, 3120, 3155
CviAII	CATG	9 cut(s)	85, 1396, 1456, 1955, 2018, 2175, 2206, 2345, 2778
CviQI	GTAC	4 cut(s)	523, 2036, 2192, 2869
DraI	TTTAAA	1 cut(s)	2287
Eam1104I	CTCTTC	5 cut(s)	377, 1359, 1380, 1731, 3078
EarI	CTCTTC	5 cut(s)	377, 1359, 1380, 1731, 3078
EciI	GGCGGA	1 cut(s)	193
Eco130I	CCWWGG	3 cut(s)	1185, 1629, 2205
Eco147I	AGGCCT	1 cut(s)	842
Eco31I	GGTCTC	1 cut(s)	2420
Eco32I	GATATC	1 cut(s)	2130
Eco47I	GGWCC	1 cut(s)	603
Eco57I	CTGAAG	2 cut(s)	1869, 2007
Eco72I	CACGTG	1 cut(s)	1802
Eco91I	GGTNACC	1 cut(s)	1130
EcoNI	CCTNNNNNAGG	1 cut(s)	288
EcoO109I	RGGNCCY	2 cut(s)	603, 1597
EcoO65I	GGTNACC	1 cut(s)	1130
EcoRI	GAATTC	2 cut(s)	338, 917
EcoRII	CCWGG	3 cut(s)	959, 1749, 2682
EcoRV	GATATC	1 cut(s)	2130
EcoT14I	CCWWGG	3 cut(s)	1185, 1629, 2205
EcoT22I	ATGCAT	1 cut(s)	586
ErhI	CCWWGG	3 cut(s)	1185, 1629, 2205
Esp3I	CGTCTC	1 cut(s)	19
FaeI	CATG	9 cut(s)	88, 1399, 1459, 1958, 2021, 2178, 2209, 2348, 2781
FalI	AAGNNNNNCTT	6 cut(s)	1622, 1654, 1649, 1681, 1770, 1802
FaqI	GGGAC	3 cut(s)	104, 1252, 1850
FatI	CATG	9 cut(s)	84, 1395, 1455, 1954, 2017, 2174, 2205, 2344, 2777
FauI	CCCGC	2 cut(s)	277, 2698
FauNDI	CATATG	3 cut(s)	862, 1149, 2815
FbaI	TGATCA	1 cut(s)	1392
FblI	GTMKAC	4 cut(s)	101, 166, 1580, 2644
FspBI	CTAG	4 cut(s)	1796, 2033, 2517, 2594
GlaI	GCGC	2 cut(s)	32, 253
GsaI	CCCAGC	2 cut(s)	1238, 2214
GsuI	CTGGAG	2 cut(s)	2081, 2937
HaeIII	GGCC	3 cut(s)	842, 1598, 2934
HapII	CCGG	4 cut(s)	162, 527, 2724, 2872
HgaI	GACGC	1 cut(s)	707
HhaI	GCGC	2 cut(s)	33, 254
Hin1I	GRCGYC	1 cut(s)	169
Hin1II	CATG	9 cut(s)	88, 1399, 1459, 1958, 2021, 2178, 2209, 2348, 2781
Hin6I	GCGC	2 cut(s)	31, 252
HinP1I	GCGC	2 cut(s)	31, 252
HincII	GTYRAC	4 cut(s)	102, 871, 1162, 2645
HindII	GTYRAC	4 cut(s)	102, 871, 1162, 2645
HindIII	AAGCTT	2 cut(s)	311, 1658
HinfI	GANTC	5 cut(s)	196, 467, 1240, 2837, 2993
HpaI	GTTAAC	1 cut(s)	1162
HpaII	CCGG	4 cut(s)	162, 527, 2724, 2872
Hpy166II	GTNNAC	8 cut(s)	102, 167, 871, 994, 1162, 1581, 2645, 2689
Hpy8I	GTNNAC	8 cut(s)	102, 167, 871, 994, 1162, 1581, 2645, 2689
Hpy99I	CGWCG	4 cut(s)	103, 106, 186, 260
HpyAV	CCTTC	7 cut(s)	457, 1418, 1588, 1936, 2106, 2465, 3100
HpyCH4III	ACNGT	5 cut(s)	13, 875, 935, 2292, 2525
HpyCH4IV	ACGT	3 cut(s)	169, 1801, 2929
HpyF10VI	GCNNNNNNNGC	5 cut(s)	135, 839, 1448, 1796, 2216
HpySE526I	ACGT	3 cut(s)	169, 1801, 2929
Hsp92I	GRCGYC	1 cut(s)	169
Hsp92II	CATG	9 cut(s)	88, 1399, 1459, 1958, 2021, 2178, 2209, 2348, 2781
HspAI	GCGC	2 cut(s)	31, 252
Ksp22I	TGATCA	1 cut(s)	1392
KspAI	GTTAAC	1 cut(s)	1162
KspI	CCGCGG	1 cut(s)	3180
LmnI	GCTCC	6 cut(s)	9, 183, 277, 1530, 1813, 3215
Lsp1109I	GCAGC	8 cut(s)	45, 119, 1339, 1711, 1778, 2190, 2341, 3206
LweI	GCATC	9 cut(s)	658, 1429, 1460, 1686, 1687, 2359, 3001, 3181, 3182
MaeI	CTAG	4 cut(s)	1796, 2033, 2517, 2594
MaeII	ACGT	3 cut(s)	169, 1801, 2929
MaeIII	GTNAC	6 cut(s)	1130, 1684, 1883, 2629, 2708, 3052
MbiI	CCGCTC	1 cut(s)	272
MfeI	CAATTG	1 cut(s)	1464
MflI	RGATCY	8 cut(s)	963, 1263, 1496, 1558, 1625, 1783, 2504, 2875
MhlI	GDGCHC	2 cut(s)	2072, 2314
MlyI	GAGTC	1 cut(s)	1234
MmeI	TCCRAC	5 cut(s)	205, 312, 1903, 2677, 3237
Mph1103I	ATGCAT	1 cut(s)	586
MroXI	GAANNNNTTC	1 cut(s)	3110
MslI	CAYNNNNRTG	3 cut(s)	1694, 1858, 2782
MspA1I	CMGCKG	1 cut(s)	3179
MspI	CCGG	4 cut(s)	162, 527, 2724, 2872
MspR9I	CCNGG	6 cut(s)	527, 961, 1751, 2684, 2725, 2873
MssI	GTTTAAAC	1 cut(s)	2287
MunI	CAATTG	1 cut(s)	1464
Mva1269I	GAATGC	3 cut(s)	477, 584, 2842
MvaI	CCWGG	3 cut(s)	961, 1751, 2684
MvnI	CGCG	4 cut(s)	31, 252, 2196, 3179
MwoI	GCNNNNNNNGC	5 cut(s)	135, 839, 1448, 1796, 2216
NciI	CCSGG	3 cut(s)	527, 2725, 2873
NcoI	CCATGG	1 cut(s)	2205
NdeI	CATATG	3 cut(s)	862, 1149, 2815
NheI	GCTAGC	1 cut(s)	1795
NlaIII	CATG	9 cut(s)	88, 1399, 1459, 1958, 2021, 2178, 2209, 2348, 2781
NlaIV	GGNNCC	5 cut(s)	1560, 1587, 1627, 2877, 2977
NmuCI	GTSAC	3 cut(s)	1130, 1883, 2629
NsiI	ATGCAT	1 cut(s)	586
NspI	RCATGY	1 cut(s)	88
PceI	AGGCCT	1 cut(s)	842
PciI	ACATGT	1 cut(s)	84
PcsI	WCGNNNNNNNCGW	2 cut(s)	35, 61
PctI	GAATGC	3 cut(s)	477, 584, 2842
PdmI	GAANNNNTTC	1 cut(s)	3110
PfeI	GAWTC	4 cut(s)	196, 467, 2837, 2993
Pfl23II	CGTACG	1 cut(s)	2191
PflMI	CCANNNNNTGG	1 cut(s)	966
PfoI	TCCNGGA	1 cut(s)	1749
PleI	GAGTC	1 cut(s)	1234
PmaCI	CACGTG	1 cut(s)	1802
PmeI	GTTTAAAC	1 cut(s)	2287
PmlI	CACGTG	1 cut(s)	1802
PpsI	GAGTC	1 cut(s)	1234
Ppu21I	YACGTR	2 cut(s)	1802, 2930
PpuMI	RGGWCCY	1 cut(s)	603
PscI	ACATGT	1 cut(s)	84
PshBI	ATTAAT	1 cut(s)	1932
Psp5II	RGGWCCY	1 cut(s)	603
Psp6I	CCWGG	3 cut(s)	959, 1749, 2682
PspCI	CACGTG	1 cut(s)	1802
PspEI	GGTNACC	1 cut(s)	1130
PspFI	CCCAGC	2 cut(s)	1234, 2210
PspGI	CCWGG	3 cut(s)	959, 1749, 2682
PspLI	CGTACG	1 cut(s)	2191
PspN4I	GGNNCC	5 cut(s)	1560, 1587, 1627, 2877, 2977
PspPI	GGNCC	2 cut(s)	603, 1597
PspPPI	RGGWCCY	1 cut(s)	603
PstNI	CAGNNNCTG	1 cut(s)	966
PsuI	RGATCY	8 cut(s)	963, 1263, 1496, 1558, 1625, 1783, 2504, 2875
RsaI	GTAC	4 cut(s)	524, 2037, 2193, 2870
RsaNI	GTAC	4 cut(s)	523, 2036, 2192, 2869
RseI	CAYNNNNRTG	3 cut(s)	1694, 1858, 2782
SacII	CCGCGG	1 cut(s)	3180
SalI	GTCGAC	2 cut(s)	100, 2643
Sau96I	GGNCC	2 cut(s)	603, 1597
SchI	GAGTC	1 cut(s)	1234
ScrFI	CCNGG	6 cut(s)	527, 961, 1751, 2684, 2725, 2873
SduI	GDGCHC	2 cut(s)	2072, 2314
SfaNI	GCATC	9 cut(s)	658, 1429, 1460, 1686, 1687, 2359, 3001, 3181, 3182
SfcI	CTRYAG	1 cut(s)	1806
Sfr303I	CCGCGG	1 cut(s)	3180
SgrBI	CCGCGG	1 cut(s)	3180
SgrDI	CGTCGACG	1 cut(s)	100
SinI	GGWCC	1 cut(s)	603
SmiMI	CAYNNNNRTG	3 cut(s)	1694, 1858, 2782
SmlI	CTYRAG	4 cut(s)	554, 1009, 1722, 2489
SmoI	CTYRAG	4 cut(s)	554, 1009, 1722, 2489
SpeI	ACTAGT	1 cut(s)	2032
SseBI	AGGCCT	1 cut(s)	842
SspI	AATATT	2 cut(s)	817, 941
SspMI	CTAG	4 cut(s)	1796, 2033, 2517, 2594
StuI	AGGCCT	1 cut(s)	842
StyD4I	CCNGG	6 cut(s)	525, 959, 1749, 2682, 2723, 2871
StyI	CCWWGG	3 cut(s)	1185, 1629, 2205
TaaI	ACNGT	5 cut(s)	13, 875, 935, 2292, 2525
TaiI	ACGT	3 cut(s)	172, 1804, 2932
TaqI	TCGA	7 cut(s)	101, 654, 1123, 2571, 2644, 2835, 3222
TauI	GCSGC	3 cut(s)	209, 1954, 2360
TfiI	GAWTC	4 cut(s)	196, 467, 2837, 2993
TscAI	CASTG	6 cut(s)	223, 478, 880, 1653, 2160, 2749
TseFI	GTSAC	3 cut(s)	1130, 1883, 2629
TseI	GCWGC	8 cut(s)	33, 107, 1327, 1699, 1766, 2178, 2354, 3194
Tsp45I	GTSAC	3 cut(s)	1130, 1883, 2629
TspGWI	ACGGA	4 cut(s)	104, 992, 1598, 2329
TspRI	CASTG	6 cut(s)	223, 478, 880, 1653, 2160, 2749
Van91I	CCANNNNNTGG	1 cut(s)	966
VpaK11BI	GGWCC	1 cut(s)	603
VspI	ATTAAT	1 cut(s)	1932
XagI	CCTNNNNNAGG	1 cut(s)	288
XapI	RAATTY	7 cut(s)	141, 338, 917, 1067, 2429, 2475, 2588
XbaI	TCTAGA	1 cut(s)	2593
XceI	RCATGY	1 cut(s)	88
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1340
XmiI	GTMKAC	4 cut(s)	101, 166, 1580, 2644
XmnI	GAANNNNTTC	1 cut(s)	3110
XspI	CTAG	4 cut(s)	1796, 2033, 2517, 2594
ZraI	GACGTC	1 cut(s)	170
Zsp2I	ATGCAT	1 cut(s)	586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.