Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Reverse (-)

13617796 .. 13626590

Gene Structure 8795 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ23158

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3261 bp

ATGGAGAGAGCAGCTCTCCTTCGCTCTTCTCTTTCCTGCACTAATAGAGCCTTCTTCTCCTTCCGCCCCCCCTCTATCCCATTCTTTCGATTCTCTCGCTCCTCCTCCTCCTCCTCTTCTTCTTCTTCTGCTCTCAGACCCCATCGCCACCGTCCGATTCTCCGACCCTCCTCCGGCCGCCCCCTCCGCCGCACCAGCCTCTTACCACTCCCCGCCGCTTCCACCCACTTCGCCACGCGCTTCTCCTCCCTCTCCCCACGCGCTGTCGCCACGCCCTTAACCCCCTCGCCGTCAGAGTTCTCTGGGGTTTCCGATGAGGTTGCGGAACAGCTGGGATTCGAGAAGGTCGCCGAAGAGTTTATAGGAGAGTGTAAATCCAAAGCTCTGCTCTTTAAGCACAAAAAGACCGGTGCTCAGGTGATCTCTGTTTCCAACGACGATGAGAACAAGGTTTTCGGCATTGTTTTCCGAACACCACCAAATGATTCGACTGGAATCCCACACATTTTGGAGCACAGTGTGTTGTGTGGTTCTAGGAAATACCCTTTGAAAGAGCCGTTTGTGGAATTGTTGAAAGGGAGCTTGAATACTTTTCTCAATGCATTCACCTATCCTGATAGGACTTGCTACCCTGTTGCCTCCACAAATTCAAAGGATTTCTATAATCTGGTCGATGTGTACCTGGATGCTGTGTTCTTTCCAAAATGTATGGAGGATTTCCAGACTTTCCAGCAGGAGGGCTGGCACTATGAGCTCAACGATCCTTCTGAAGACATATCTTATAAAGGAGTTGTTTTCAATGAGATGAAAGGGGTGTACTCCCAGCCTGATAATATATTAGGGCGGATTGCTCAACAGGCTCTTTTTCCAGACAACACCTATGGTGTGGACAGTGGAGGTGATCCAAAAGTTATTCCCAAGCTGACTTATGAGGAATTTAAGGAATTTCACCGTAAATATTACCATCCGAGCAATGCCAGGATTTGGTTCTATGGAGATGATGATCCAACTGAGCGCCTACGCATTTTAAGTGAGTACCTAGACATGTTTGATGCAAGTTCAGCTCCAAATGAATCAAGGGTTCAAACACAAAAACTATTTTCGGAACCAGTTAGGATTTCTGAGACATATCCTTCTGGTGAAGGCGGCGACTTAAAGAAAAAAGGCATGGTATGCATCAATTGGCTGCTCTCTGAGAAACCCCTGGACTTGGAAACTGAGCTTGCCCTTGGGTTTTTAGATCATCTTATGTTGGGAACTCCTGCGTCTCCATTGAGGAAAATATTGCTGGAAAGTGGCTTGGGAGAAGCTATCATTGGAGGCGGGGTTGAAGATGAGCTTCTTCAACCTCAATTTAGTATCGGGTTGAAGGGTGTTTCTCAAGATAATATTCAAAAGGTAGAAGAATTAGTAATGAGTACACTTAAAAAATTAGCGGAAGAAGGGTTCGATACAGCTGCTGTAGAAGCATCCATGAATACCATTGAGTTTTCTCTCAGGGAAAACAACACCGGATCATTTCCTCGTGGTTTGTCCTTAATGCTTCGATCCATGGGAAAGTGGATATATGATATGGATCCATTTCAGCCATTGAAGTATGAGAAACCTTTACTGGCACTAAAAGCAAGAATAGAACAGGAAGGCTCTAAAGCCGTTTTCTCTCCTTTAATAGAGAAATTTATTTTGAACAATCTTCATCGAGTTGTGGTAGAAATGCAGCCTGATCCAGAAAAAGCTTCTCGTGATGAAGCAGCTGAGAAAGAAATTTTGGAGAAAGTAAAAGCAGGTATGACGGAAGAAGATCTTGCTGAGCTAGCACGCGCTACACAAGAACTGAAACTGAAGCAGGAAACTCCTGATCCACCAGAAGCTCTGAGAAGTGTTCCAAGCCTCTCTCTGCTTGACATTCCAAAGGAACCTATTCCTGTTCCCACAGAGGTTGGGGATATCAATGGAGTTAAAGTTTTGCAGCATGACCTTTTTACAAATGATGTTCTCTACACTGAAGTTGTGTTCAATATGAGTTCACTCAAGCAAGAGCTCCTTCCTTTGGTACCTCTTTTTTGTCAATCATTGCTGGAGATGGGAACCAAAGATTTGAGTTTTGTGGAACTTAATCAGTTGATTGGAAGAAAAACTGGAGGGATATCAGTTTATCCACTGACATCATCTGTCCGGGGCAAGAAGGATCCCTGTAGCCATATAATTGTTCGAGGCAAAGCCATGGCAGGACGTGCGGCTGATCTATTTCATCTGATCAACTGTATTCTTCAAGAAGTCCAATTTACAGACCAGCAGCGGTTTAAGCAGTTTGTTTCCCAAAGCAAAGCTAGAATGGAGAACCGATTGAGAGGTAGCGGTCATGGAATTGCTGCTGCAAGGATGGATGCAAAGCTAAATGTTGCGGGGTGGATTTCTGAACAAATGGGTGGTTTCAGTTACTTGGAGTTTCTACAAGCTCTTGAAGAAAAAGTTGATAATGATTGGGAGAGAATATCTTCATCTCTTGAGGAGATTCGCAAGTCCTTACTTTCACAGGAGGGTTGCTTGATTAATATGACTGCTGAAGGAAAGAATCTTACCAACTCTGAGAAGTTTGTTGGCGAATTTCTTAATTTGCTGCCTCACAAATCTCCCCTTGCAACAACTACTTGGAATGCTCGATTACCTTCTATAAATGAAGCCATTGTTATACCTACTCAGGTTAATTATGTTGGAAAAGCAGCCAACATTTATGACACTGGGTATCAACTTAATGGAAGTGCATATGTTATCTCTAAATACATAAGTAATACATGGTTGTGGGATCGTGTACGAGTTAGTGGTGGAGCATATGGAGGCTTTTGCGACTTCGATAGTCACTCCGGTGTGTTCTCCTTCTTATCTTATCGTGACCCCAACTTGTTAAAGACACTTGATATATATGATGGAACTGGGGAATTTCTACGTCAACTAGATTTGGATGAAGAAACTCTCACAAAGTCCATCATTGGAACAATTGGAGATGTTGATTCATACCAGCTTCCTGATGCCAAAGGTTATAGTAGTTTGATGCGGTATTTATTGGGAGTTTCAGATGAGGAAAGGAAAATTAGGCGAGAAGAGATATTGTCAACAAGCTTGAAGGACTTCAAGGAGTTTGCAAATGCAATTGATGCAGTTAAAGACAAAGGGGTTGCTGTTGCAGTGGCATCTCCAGATGATGTTGATGCAGCACAAAAGGAAAGATCAAACTTATTTGAAGTGAAGAAAGCCCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

1086

Amino Acids

121.32

Weight (kDa)

5.43

Isoelectric Point (pI)

44.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	160 - 238	2.8e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	307 - 493	6e-27	Peptidase M16 inactive domain
M16C_assoc	PF08367	572 - 821	2.1e-76	Peptidase M16C associated
PreP_C	PF22516	893 - 1065	6.8e-81	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	783
Acc36I	ACCTGC	1 cut(s)	1775
Acc65I	GGTACC	1 cut(s)	2053
AccB1I	GGYRCC	1 cut(s)	2053
AccB7I	CCANNNNNTGG	1 cut(s)	984
AccII	CGCG	3 cut(s)	238, 261, 1821
AcoI	YGGCCR	1 cut(s)	175
AcsI	RAATTY	7 cut(s)	646, 935, 944, 1676, 1764, 2606, 2939
AcuI	CTGAAG	4 cut(s)	789, 1862, 2025, 2586
AdeI	CACNNNGTG	1 cut(s)	520
AfaI	GTAC	6 cut(s)	680, 818, 1037, 1420, 2055, 2814
AfiI	CCNNNNNNNGG	5 cut(s)	173, 736, 984, 1210, 2050
AflIII	ACRYGT	1 cut(s)	1044
AgeI	ACCGGT	1 cut(s)	407
AjiI	CACGTC	1 cut(s)	2234
AjnI	CCWGG	3 cut(s)	681, 977, 1203
AjuI	GAANNNNNNNTTGG	4 cut(s)	1299, 1331, 1751, 1783
AleI	CACNNNNGTG	1 cut(s)	2865
AloI	GAACNNNNNNTCC	4 cut(s)	677, 709, 1430, 1462
Alw21I	GWGCWC	4 cut(s)	415, 516, 756, 2043
Alw26I	GTCTC	2 cut(s)	1118, 1272
AlwNI	CAGNNNCTG	1 cut(s)	1460
AoxI	GGCC	1 cut(s)	175
ApoI	RAATTY	7 cut(s)	646, 935, 944, 1676, 1764, 2606, 2939
AseI	ATTAAT	1 cut(s)	2553
AsiGI	ACCGGT	1 cut(s)	407
Asp700I	GAANNNNTTC	3 cut(s)	1920, 2497, 3128
Asp718I	GGTACC	1 cut(s)	2053
AspLEI	GCGC	4 cut(s)	240, 263, 1017, 1823
AsuC2I	CCSGG	1 cut(s)	2177
AsuHPI	GGTGA	5 cut(s)	430, 598, 911, 941, 1151
AsuNHI	GCTAGC	1 cut(s)	1813
BamHI	GGATCC	2 cut(s)	1576, 2188
BanI	GGYRCC	1 cut(s)	2053
BanII	GRGCYC	2 cut(s)	756, 2043
BauI	CACGAG	2 cut(s)	1524, 1740
BbsI	GAAGAC	1 cut(s)	777
Bbv12I	GWGCWC	4 cut(s)	415, 516, 756, 2043
BccI	CCATC	6 cut(s)	150, 972, 2077, 2377, 2921, 2993
BceAI	ACGGC	3 cut(s)	274, 541, 1637
BcgI	CGANNNNNNTGC	4 cut(s)	302, 336, 1439, 1473
BciT130I	CCWGG	3 cut(s)	683, 979, 1205
BclI	TGATCA	1 cut(s)	2256
BcnI	CCSGG	1 cut(s)	2177
BcoDI	GTCTC	2 cut(s)	1118, 1272
BfaI	CTAG	5 cut(s)	534, 1040, 1814, 2331, 2954
BfmI	CTRYAG	2 cut(s)	1461, 2194
BfoI	RGCGCY	1 cut(s)	1018
BfuAI	ACCTGC	1 cut(s)	1775
BglII	AGATCT	1 cut(s)	1801
BlpI	GCTNAGC	1 cut(s)	1809
Bme1390I	CCNGG	4 cut(s)	683, 979, 1205, 2177
BmgBI	CACGTC	1 cut(s)	2234
BmiI	GGNNCC	6 cut(s)	1107, 1578, 1917, 2055, 2089, 2190
BmrFI	CCNGG	4 cut(s)	683, 979, 1205, 2177
BmrI	ACTGGG	2 cut(s)	2751, 2943
BmtI	GCTAGC	1 cut(s)	1817
BmuI	ACTGGG	2 cut(s)	2751, 2943
BpiI	GAAGAC	1 cut(s)	777
BplI	GAGNNNNNCTC	2 cut(s)	30, 32
BpmI	CTGGAG	3 cut(s)	2099, 2160, 3180
Bpu10I	CCTNAGC	1 cut(s)	414
Bpu1102I	GCTNAGC	1 cut(s)	1809
BpuEI	CTTGAG	3 cut(s)	1365, 2015, 2528
BpuMI	CCSGG	1 cut(s)	2177
BsaBI	GATNNNNATC	2 cut(s)	1002, 1575
BsaJI	CCNNGG	5 cut(s)	1203, 1228, 1551, 2176, 2223
BsaWI	WCCGGW	3 cut(s)	407, 1511, 2864
BsaXI	ACNNNNNCTCC	4 cut(s)	1311, 1341, 1947, 1977
Bsc4I	CCNNNNNNNGG	5 cut(s)	173, 736, 984, 1210, 2050
Bse118I	RCCGGY	1 cut(s)	407
Bse1I	ACTGG	6 cut(s)	496, 1109, 1617, 2143, 2746, 2938
Bse3DI	GCAATG	2 cut(s)	979, 2072
Bse8I	GATNNNNATC	2 cut(s)	1002, 1575
BseBI	CCWGG	3 cut(s)	683, 979, 1205
BseDI	CCNNGG	5 cut(s)	1203, 1228, 1551, 2176, 2223
BseGI	GGATG	6 cut(s)	691, 964, 1469, 2388, 2392, 2968
BseJI	GATNNNNATC	2 cut(s)	1002, 1575
BseLI	CCNNNNNNNGG	5 cut(s)	173, 736, 984, 1210, 2050
BseMI	GCAATG	2 cut(s)	979, 2072
BseNI	ACTGG	6 cut(s)	496, 1109, 1617, 2143, 2746, 2938
BseRI	GAGGAG	8 cut(s)	91, 94, 97, 100, 103, 160, 235, 2525
BseX3I	CGGCCG	1 cut(s)	175
BseYI	CCCAGC	2 cut(s)	331, 822
BsgI	GTGCAG	1 cut(s)	22
Bsh1236I	CGCG	3 cut(s)	238, 261, 1821
Bsh1285I	CGRYCG	1 cut(s)	178
BshFI	GGCC	1 cut(s)	177
BshNI	GGYRCC	1 cut(s)	2053
BshTI	ACCGGT	1 cut(s)	407
BsiEI	CGRYCG	1 cut(s)	178
BsiHKAI	GWGCWC	4 cut(s)	415, 516, 756, 2043
BsiSI	CCGG	5 cut(s)	174, 408, 1512, 2176, 2865
BslI	CCNNNNNNNGG	5 cut(s)	173, 736, 984, 1210, 2050
BsmAI	GTCTC	2 cut(s)	1118, 1272
BsmBI	CGTCTC	1 cut(s)	1272
BsmI	GAATGC	2 cut(s)	602, 2662
BsnI	GGCC	1 cut(s)	177
Bsp1286I	GDGCHC	4 cut(s)	415, 516, 756, 2043
Bsp1720I	GCTNAGC	1 cut(s)	1809
Bsp19I	CCATGG	2 cut(s)	1551, 2223
BspANI	GGCC	1 cut(s)	177
BspFNI	CGCG	3 cut(s)	238, 261, 1821
BspLI	GGNNCC	6 cut(s)	1107, 1578, 1917, 2055, 2089, 2190
BspMI	ACCTGC	1 cut(s)	1775
BspOI	GCTAGC	1 cut(s)	1817
BspQI	GCTCTTC	1 cut(s)	31
BspT107I	GGYRCC	1 cut(s)	2053
BsrDI	GCAATG	2 cut(s)	979, 2072
BsrFI	RCCGGY	1 cut(s)	407
BsrI	ACTGG	6 cut(s)	496, 1109, 1617, 2143, 2746, 2938
BssAI	RCCGGY	1 cut(s)	407
BssECI	CCNNGG	5 cut(s)	1203, 1228, 1551, 2176, 2223
BssSI	CACGAG	2 cut(s)	1524, 1740
BssT1I	CCWWGG	3 cut(s)	1228, 1551, 2223
Bst2BI	CACGAG	2 cut(s)	1524, 1740
Bst2UI	CCWGG	3 cut(s)	683, 979, 1205
Bst4CI	ACNGT	5 cut(s)	152, 518, 893, 953, 2264
Bst6I	CTCTTC	4 cut(s)	31, 121, 348, 3096
BstAPI	GCANNNNNTGC	3 cut(s)	1173, 2234, 3155
BstC8I	GCNNGC	4 cut(s)	743, 1224, 1815, 1819
BstDSI	CCRYGG	2 cut(s)	1551, 2223
BstF5I	GGATG	6 cut(s)	691, 964, 1469, 2388, 2392, 2968
BstFNI	CGCG	3 cut(s)	238, 261, 1821
BstH2I	RGCGCY	1 cut(s)	1018
BstHHI	GCGC	4 cut(s)	240, 263, 1017, 1823
BstMAI	GTCTC	2 cut(s)	1118, 1272
BstMCI	CGRYCG	1 cut(s)	178
BstNI	CCWGG	3 cut(s)	683, 979, 1205
BstNSI	RCATGY	1 cut(s)	1048
BstSCI	CCNGG	4 cut(s)	681, 977, 1203, 2175
BstSFI	CTRYAG	2 cut(s)	1461, 2194
BstUI	CGCG	3 cut(s)	238, 261, 1821
BstV2I	GAAGAC	1 cut(s)	777
BstX2I	RGATCY	3 cut(s)	1576, 1801, 2188
BstYI	RGATCY	3 cut(s)	1576, 1801, 2188
BstZI	CGGCCG	1 cut(s)	175
BsuRI	GGCC	1 cut(s)	177
BtgI	CCRYGG	2 cut(s)	1551, 2223
BtgZI	GCGATG	1 cut(s)	128
BtrI	CACGTC	1 cut(s)	2234
BtsCI	GGATG	6 cut(s)	691, 964, 1469, 2388, 2392, 2968
BtsI	GCAGTG	1 cut(s)	3192
BtsIMutI	CAGTG	6 cut(s)	523, 898, 2001, 2159, 2739, 3192
BveI	ACCTGC	1 cut(s)	1775
Cac8I	GCNNGC	4 cut(s)	743, 1224, 1815, 1819
CaiI	CAGNNNCTG	1 cut(s)	1460
CfoI	GCGC	4 cut(s)	240, 263, 1017, 1823
Cfr10I	RCCGGY	1 cut(s)	407
CseI	GACGC	1 cut(s)	1254
Csp6I	GTAC	6 cut(s)	679, 817, 1036, 1419, 2054, 2813
CspAI	ACCGGT	1 cut(s)	407
CspCI	CAANNNNNGTGG	4 cut(s)	489, 524, 1921, 1956
CviAII	CATG	8 cut(s)	1045, 1168, 1474, 1552, 1973, 2224, 2363, 2796
CviQI	GTAC	6 cut(s)	679, 817, 1036, 1419, 2054, 2813
DraIII	CACNNNGTG	1 cut(s)	520
EaeI	YGGCCR	1 cut(s)	175
EagI	CGGCCG	1 cut(s)	175
Eam1104I	CTCTTC	4 cut(s)	31, 121, 348, 3096
EarI	CTCTTC	4 cut(s)	31, 121, 348, 3096
EciI	GGCGGA	3 cut(s)	53, 176, 859
Ecl136II	GAGCTC	2 cut(s)	754, 2041
EclXI	CGGCCG	1 cut(s)	175
Eco130I	CCWWGG	3 cut(s)	1228, 1551, 2223
Eco24I	GRGCYC	2 cut(s)	756, 2043
Eco32I	GATATC	2 cut(s)	1948, 2148
Eco52I	CGGCCG	1 cut(s)	175
Eco53kI	GAGCTC	2 cut(s)	754, 2041
Eco57I	CTGAAG	4 cut(s)	789, 1862, 2025, 2586
EcoICRI	GAGCTC	2 cut(s)	754, 2041
EcoRII	CCWGG	3 cut(s)	681, 977, 1203
EcoRV	GATATC	2 cut(s)	1948, 2148
EcoT14I	CCWWGG	3 cut(s)	1228, 1551, 2223
EcoT22I	ATGCAT	2 cut(s)	604, 1178
EcoT38I	GRGCYC	2 cut(s)	756, 2043
ErhI	CCWWGG	3 cut(s)	1228, 1551, 2223
Esp3I	CGTCTC	1 cut(s)	1272
FaeI	CATG	8 cut(s)	1048, 1171, 1477, 1555, 1976, 2227, 2366, 2799
FalI	AAGNNNNNCTT	6 cut(s)	1323, 1355, 1788, 1820, 2028, 2060
FatI	CATG	8 cut(s)	1044, 1167, 1473, 1551, 1972, 2223, 2362, 2795
FauI	CCCGC	3 cut(s)	220, 1316, 2398
FauNDI	CATATG	2 cut(s)	2767, 2833
FbaI	TGATCA	1 cut(s)	2256
FokI	GGATG	6 cut(s)	698, 951, 1456, 2395, 2399, 2975
FriOI	GRGCYC	2 cut(s)	756, 2043
FspBI	CTAG	5 cut(s)	534, 1040, 1814, 2331, 2954
GlaI	GCGC	4 cut(s)	239, 262, 1016, 1822
GsaI	CCCAGC	2 cut(s)	335, 826
GsuI	CTGGAG	3 cut(s)	2099, 2160, 3180
HaeII	RGCGCY	1 cut(s)	1018
HaeIII	GGCC	1 cut(s)	177
HapII	CCGG	5 cut(s)	174, 408, 1512, 2176, 2865
HgaI	GACGC	1 cut(s)	1254
HhaI	GCGC	4 cut(s)	240, 263, 1017, 1823
Hin1II	CATG	8 cut(s)	1048, 1171, 1477, 1555, 1976, 2227, 2366, 2799
Hin6I	GCGC	4 cut(s)	238, 261, 1015, 1821
HinP1I	GCGC	4 cut(s)	238, 261, 1015, 1821
HincII	GTYRAC	2 cut(s)	2951, 3114
HindII	GTYRAC	2 cut(s)	2951, 3114
HindIII	AAGCTT	2 cut(s)	1734, 3118
HinfI	GANTC	9 cut(s)	90, 157, 336, 485, 495, 1073, 2515, 2575, 3011
HpaII	CCGG	5 cut(s)	174, 408, 1512, 2176, 2865
HphI	GGTGA	5 cut(s)	430, 598, 911, 941, 1151
Hpy166II	GTNNAC	8 cut(s)	679, 817, 889, 1421, 2027, 2813, 2951, 3114
Hpy8I	GTNNAC	8 cut(s)	679, 817, 889, 1421, 2027, 2813, 2951, 3114
Hpy99I	CGWCG	1 cut(s)	440
HpyCH4III	ACNGT	5 cut(s)	152, 518, 893, 953, 2264
HpyCH4IV	ACGT	2 cut(s)	2233, 2947
HpySE526I	ACGT	2 cut(s)	2233, 2947
Hsp92II	CATG	8 cut(s)	1048, 1171, 1477, 1555, 1976, 2227, 2366, 2799
HspAI	GCGC	4 cut(s)	238, 261, 1015, 1821
KpnI	GGTACC	1 cut(s)	2057
Ksp22I	TGATCA	1 cut(s)	2256
LguI	GCTCTTC	1 cut(s)	31
LmnI	GCTCC	6 cut(s)	104, 511, 579, 1069, 2046, 2828
MaeI	CTAG	5 cut(s)	534, 1040, 1814, 2331, 2954
MaeII	ACGT	2 cut(s)	2233, 2947
MaeIII	GTNAC	3 cut(s)	2438, 2858, 2891
MfeI	CAATTG	3 cut(s)	1180, 2997, 3150
MflI	RGATCY	3 cut(s)	1576, 1801, 2188
MhlI	GDGCHC	4 cut(s)	415, 516, 756, 2043
MmeI	TCCRAC	4 cut(s)	187, 456, 1031, 2695
Mph1103I	ATGCAT	2 cut(s)	604, 1178
MroXI	GAANNNNTTC	3 cut(s)	1920, 2497, 3128
MslI	CAYNNNNRTG	2 cut(s)	2800, 2865
MspA1I	CMGCKG	4 cut(s)	331, 1457, 1754, 2299
MspI	CCGG	5 cut(s)	174, 408, 1512, 2176, 2865
MspR9I	CCNGG	4 cut(s)	683, 979, 1205, 2177
MunI	CAATTG	3 cut(s)	1180, 2997, 3150
Mva1269I	GAATGC	2 cut(s)	602, 2662
MvaI	CCWGG	3 cut(s)	683, 979, 1205
MvnI	CGCG	3 cut(s)	238, 261, 1821
NciI	CCSGG	1 cut(s)	2177
NcoI	CCATGG	2 cut(s)	1551, 2223
NdeI	CATATG	2 cut(s)	2767, 2833
NheI	GCTAGC	1 cut(s)	1813
NlaIII	CATG	8 cut(s)	1048, 1171, 1477, 1555, 1976, 2227, 2366, 2799
NlaIV	GGNNCC	6 cut(s)	1107, 1578, 1917, 2055, 2089, 2190
NmuCI	GTSAC	2 cut(s)	2858, 2891
NsiI	ATGCAT	2 cut(s)	604, 1178
NspI	RCATGY	1 cut(s)	1048
OliI	CACNNNNGTG	1 cut(s)	2865
PciI	ACATGT	1 cut(s)	1044
PciSI	GCTCTTC	1 cut(s)	31
PctI	GAATGC	2 cut(s)	602, 2662
PdmI	GAANNNNTTC	3 cut(s)	1920, 2497, 3128
PfeI	GAWTC	9 cut(s)	90, 157, 336, 485, 495, 1073, 2515, 2575, 3011
PflMI	CCANNNNNTGG	1 cut(s)	984
PinAI	ACCGGT	1 cut(s)	407
PscI	ACATGT	1 cut(s)	1044
PshBI	ATTAAT	1 cut(s)	2553
PsiI	TTATAA	1 cut(s)	783
Psp124BI	GAGCTC	2 cut(s)	756, 2043
Psp6I	CCWGG	3 cut(s)	681, 977, 1203
PspFI	CCCAGC	2 cut(s)	331, 822
PspGI	CCWGG	3 cut(s)	681, 977, 1203
PspN4I	GGNNCC	6 cut(s)	1107, 1578, 1917, 2055, 2089, 2190
PstNI	CAGNNNCTG	1 cut(s)	1460
PsuI	RGATCY	3 cut(s)	1576, 1801, 2188
PvuII	CAGCTG	3 cut(s)	331, 1457, 1754
RsaI	GTAC	6 cut(s)	680, 818, 1037, 1420, 2055, 2814
RsaNI	GTAC	6 cut(s)	679, 817, 1036, 1419, 2054, 2813
RseI	CAYNNNNRTG	2 cut(s)	2800, 2865
SacI	GAGCTC	2 cut(s)	756, 2043
SapI	GCTCTTC	1 cut(s)	31
ScrFI	CCNGG	4 cut(s)	683, 979, 1205, 2177
SduI	GDGCHC	4 cut(s)	415, 516, 756, 2043
SfcI	CTRYAG	2 cut(s)	1461, 2194
SmiMI	CAYNNNNRTG	2 cut(s)	2800, 2865
SmlI	CTYRAG	3 cut(s)	1380, 2030, 2507
SmoI	CTYRAG	3 cut(s)	1380, 2030, 2507
SspI	AATATT	3 cut(s)	959, 1284, 1390
SspMI	CTAG	5 cut(s)	534, 1040, 1814, 2331, 2954
SstI	GAGCTC	2 cut(s)	756, 2043
StyD4I	CCNGG	4 cut(s)	681, 977, 1203, 2175
StyI	CCWWGG	3 cut(s)	1228, 1551, 2223
TaaI	ACNGT	5 cut(s)	152, 518, 893, 953, 2264
TaiI	ACGT	2 cut(s)	2236, 2950
TatI	WGTACW	2 cut(s)	816, 1418
TauI	GCSGC	5 cut(s)	180, 192, 218, 1149, 2240
TfiI	GAWTC	9 cut(s)	90, 157, 336, 485, 495, 1073, 2515, 2575, 3011
TscAI	CASTG	6 cut(s)	523, 898, 2008, 2166, 2746, 3192
TseFI	GTSAC	2 cut(s)	2858, 2891
Tsp45I	GTSAC	2 cut(s)	2858, 2891
TspGWI	ACGGA	1 cut(s)	1808
TspRI	CASTG	6 cut(s)	523, 898, 2008, 2166, 2746, 3192
Van91I	CCANNNNNTGG	1 cut(s)	984
VspI	ATTAAT	1 cut(s)	2553
XapI	RAATTY	7 cut(s)	646, 935, 944, 1676, 1764, 2606, 2939
XceI	RCATGY	1 cut(s)	1048
XmnI	GAANNNNTTC	3 cut(s)	1920, 2497, 3128
XspI	CTAG	5 cut(s)	534, 1040, 1814, 2331, 2954
Zsp2I	ATGCAT	2 cut(s)	604, 1178

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.