Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Forward (+)

16578700 .. 16586892

Gene Structure 8193 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1078000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGGCTGATAAGACCTACTACTCTGGTGCTTGCACAACTACAAAGGAATTGTATAATCTCATTGATGTAATCCTGGATGCCGTCTTCTTTCCTAAATGTGTGGAGGACGTCCAGATTTTCCAGGAGCAGGGTTGGCATTACGAGCTCAACGACCCTTCTGGAGATATATCTTATCAAGGCGTTGTCTTCAATAAGATGAAAGAGTTCTATTCTCGGCCTGGGCCTGATAATGTACTGAGGCAGGCTGCTTGGCAGGGCCTTTTTCCAGACAGTACCTACGGGTTTGACTGTGCAGGGGATCCAAACATTATTCCCCAACTGACATTTGAGAACTTCAAGGAGTTCCACTGGAAAAACTACCATCCGAGCAACGCAAAGATATGGTTTTATGGAGATGACGATCCAACTGAACGCCTGCACATTTTAAGTGAGTACTTAGACATGTATGATGCAAGTTCAGCTCCAAATGTATCAAGGGTTAAACCACAAAGGCTATTTTCAGAGTCAGTTAGGATTTTAAAGAACTATCCTTCTGATAAATCCGGTGATTTAGAGAAGGAAAATGTGGTATGCCTCAGTTGGATGCTCTGTGATAAGCCCTTAGACACAGAAACTGAGCAAACAATTGATGTTTTGGTTTATTTCATGATGGGAAACTTGACACGAATCTTGCAGGAATATGGTTATCATGTTTATATCGAGATTGTTCATCTCCTTCAACCTGTATTTCGCATTGCGGTGTTTAATGTTTCTTGCGATGACATTTTAAAGGTAGAACTATTTGTCACAAGTATACTTGTAGTATTTGTCGAGGAAGGCTTTGATACACATTATGTAGAAGCATCCATAACTGCAATGGAGTTGTTCATTCTCAGAGGAGGCCTTAAAAAATCTCGAGGCTTGACCTTAATGCTTCTGTCCCTAGATAAATGGATACATGAGATGAAACCCTTTGAGCCTTTGAAGTTTGGAAAATCTTTAATGGAACTGAATGCCAGAATACAAAAGGAAGGATCTAAGGCTGTCCTCTCTCCTGTAATTGAGAAATTCATATTGAAGAATCCTCATAAAGTTGTGGTAGCAATGCGGCCTGATCCACAAAAGGTTTCTTCTGATGAAAAAGCTGAGAAAGAAATATTGAGGAAAGTTAAAGGTGGTATGAGAGAAGAAGATCTTGCTGAGCTGGCACGTGCTACCCAGGAGCTGCAACTGAAGCAGAAAACTCCTGACCCACCAGAAGCTTTGAGAAGTGTTCCAAGCCTATCTCTGCTAGACCTTCCAAAGGAACCTATTCATGTTCCCACAGAGGTTGCGTATGTCAATGGAGTTAAAGTATTGCAGCATGAACTCTTCACGAATGATGTCCTTTACACTGAAATTGTGTTCAATATGGGTTCACTAAAGCAAGAGCTTCTTCCTTTGGTACCTATTTTCTGTTTCACGTTACTGAAGATGGATACAAAAGACATGAGTTCTGATGAACTTCGTCACTTGATTTCAAGAAAAACGGGAGGACTAACAATCGATCCAATGATTTTGTCGGTACGGTGCAAGAAAGATCCATGCGGCCATGTAGTTGTTGGAGGGAAAGTCATGGCAGGACGTGCTGAAGACCTATTTGAACTGATCAAGATTGTTCTTCAAGAAGTACAGTTTACAGACAAGAGGCGGTTTAAGAAGATAGTTTCCCAGTTTATAGTGAAGTACGAAGAGACTGCTAATTATCATGAAATTGCTGCTGCGAGGATGGAAGCAAAGCTAAATATTTCTGGGTGGATTCGTGAACAACTTTTTGGTATCAGTCAATTGGAGTTTCTGCAAGTGCTTGGAGAGAAAGTTGATCAAGATTGGGATGGCATTTCTTCATCCCTAGAGGAGATTCGGAAGTCCATAATTTGTAGAAAAGGTTGCCTCATAAATATGATTTCTGAAAGGAAGAATCTTACCAAATCTGAGAAGTTTATTAAGAAATTTCTGGATTTGCTGCCTCATAAGTCTCCATTTGCAAGAAGAACTACATGGAAAGGTAGACTACCTTCCGAAAATGAGGCCATCATAATGCCTATTAAGGTTAATTATGTTGGAAAGTCTGTTAACATTCATGACACTGGTTATCAGCTTAATGGAAGTACACATGTTATCTCCAAATTTATTGATGATGCATGGTTGTGTGATCGGGTAGGAATTAGCGGTGGAGCATCTGCTTCATGTTACTTTGATATTTACACAGGAGTATTCTCTTTCTTGTCTGATGGGGACCCCAAGTTGTTAAAGACGCTTGACGTATATGATGGAACTGGAGATCTTCTACGACAACTAGAAATAGATGATAATACTCTTAGAAGGGCCATCATTCAGGCACTCAGAGATGATTCGTGCCCGCTTCGTGATGGAAAAAGTTGTAGTAGTTTCCTGGAGGACTATTTAATGGGAGTCACAGAAGAAGAAAGGCAAAAGAGACATAAAGAGATATTGTCAACCAGCTTGAAGGATTTCAAGAAATTTGCAGATGCACTCGACGCAGTTAAATATAAGGGGGTTGTGGTTGCAGTGGGATCGCCATTGGATATCAAAGCAGCAGTAAAGAAGCGACCTAAACTCTTCAAACAATTGATAGCACCTCTCTTCACTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

879

Amino Acids

100.06

Weight (kDa)

6.21

Isoelectric Point (pI)

46.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16_C	PF05193	107 - 284	1.7e-12	Peptidase M16 inactive domain
M16C_assoc	PF08367	362 - 609	2.3e-57	Peptidase M16C associated
PreP_C	PF22516	682 - 853	1.2e-50	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	111
Acc65I	GGTACC	1 cut(s)	1423
AccB1I	GGYRCC	1 cut(s)	1423
AccI	GTMKAC	2 cut(s)	793, 2029
AciI	CCGC	6 cut(s)	737, 1087, 1566, 1669, 2190, 2381
AclWI	GGATC	8 cut(s)	293, 306, 395, 1021, 1088, 1520, 1553, 2563
AcoI	YGGCCR	1 cut(s)	1567
AcsI	RAATTY	4 cut(s)	1046, 1970, 2147, 2501
AcuI	CTGAAG	3 cut(s)	1232, 1469, 1629
AcvI	CACGTG	1 cut(s)	1190
AcyI	GRCGYC	1 cut(s)	108
AfaI	GTAC	8 cut(s)	234, 274, 434, 1425, 1545, 1650, 1706, 2131
AfiI	CCNNNNNNNGG	2 cut(s)	127, 1282
AflIII	ACRYGT	2 cut(s)	441, 2134
AjiI	CACGTC	1 cut(s)	1604
AjnI	CCWGG	5 cut(s)	72, 120, 217, 1197, 2412
AjuI	GAANNNNNNNTTGG	2 cut(s)	1776, 1808
Alw21I	GWGCWC	1 cut(s)	147
Alw26I	GTCTC	3 cut(s)	1706, 2001, 2452
AlwI	GGATC	8 cut(s)	293, 306, 395, 1021, 1088, 1520, 1553, 2563
AlwNI	CAGNNNCTG	2 cut(s)	614, 1204
Ama87I	CYCGRG	1 cut(s)	894
AoxI	GGCC	8 cut(s)	215, 221, 256, 880, 1088, 1567, 2049, 2346
ApeKI	GCWGC	7 cut(s)	245, 1204, 1339, 1736, 1739, 1984, 2576
ApoI	RAATTY	4 cut(s)	1046, 1970, 2147, 2501
Asp700I	GAANNNNTTC	3 cut(s)	203, 1290, 2492
Asp718I	GGTACC	1 cut(s)	1423
AspS9I	GGNCC	4 cut(s)	221, 256, 2257, 2346
AsuHPI	GGTGA	1 cut(s)	557
AvaI	CYCGRG	1 cut(s)	894
AvaII	GGWCC	1 cut(s)	2257
BaeGI	GKGCMC	1 cut(s)	2381
BamHI	GGATCC	1 cut(s)	298
BanI	GGYRCC	1 cut(s)	1423
BanII	GRGCYC	1 cut(s)	147
BarI	GAAGNNNNNNTAC	2 cut(s)	2020, 2052
BbrPI	CACGTG	1 cut(s)	1190
BbsI	GAAGAC	3 cut(s)	76, 178, 1617
Bbv12I	GWGCWC	1 cut(s)	147
BbvI	GCAGC	7 cut(s)	232, 1191, 1351, 1723, 1726, 1971, 2588
BceAI	ACGGC	1 cut(s)	65
BcgI	CGANNNNNNTGC	4 cut(s)	1697, 1731, 2538, 2572
BciT130I	CCWGG	5 cut(s)	74, 122, 219, 1199, 2414
BciVI	GTATCC	2 cut(s)	927, 1450
BclI	TGATCA	2 cut(s)	1626, 1840
BcoDI	GTCTC	3 cut(s)	1706, 2001, 2452
BfaI	CTAG	4 cut(s)	923, 1271, 1871, 2318
BfuI	GTATCC	2 cut(s)	927, 1450
BglII	AGATCT	2 cut(s)	1171, 2302
BisI	GCNGC	9 cut(s)	246, 1088, 1205, 1340, 1567, 1737, 1740, 1985, 2577
BlpI	GCTNAGC	1 cut(s)	1179
BlsI	GCNGC	9 cut(s)	247, 1089, 1206, 1341, 1568, 1738, 1741, 1986, 2578
BmcAI	AGTACT	1 cut(s)	434
Bme1390I	CCNGG	5 cut(s)	74, 122, 219, 1199, 2414
Bme18I	GGWCC	1 cut(s)	2257
BmeT110I	CYCGRG	1 cut(s)	894
BmgBI	CACGTC	1 cut(s)	1604
BmgT120I	GGNCC	4 cut(s)	221, 256, 2257, 2346
BmiI	GGNNCC	5 cut(s)	300, 1287, 1425, 2258, 2259
BmrFI	CCNGG	5 cut(s)	74, 122, 219, 1199, 2414
BmrI	ACTGGG	1 cut(s)	1684
BmsI	GCATC	7 cut(s)	67, 439, 573, 851, 2149, 2207, 2500
BmuI	ACTGGG	1 cut(s)	1684
BpiI	GAAGAC	3 cut(s)	76, 178, 1617
BpmI	CTGGAG	3 cut(s)	180, 2319, 2435
Bpu1102I	GCTNAGC	1 cut(s)	1179
Bsa29I	ATCGAT	1 cut(s)	1524
BsaAI	YACGTR	1 cut(s)	1190
BsaBI	GATNNNNATC	1 cut(s)	399
BsaHI	GRCGYC	1 cut(s)	108
BsaJI	CCNNGG	2 cut(s)	218, 1197
BsaWI	WCCGGW	1 cut(s)	542
BsaXI	ACNNNNNCTCC	6 cut(s)	1317, 1347, 1575, 1605, 1821, 1851
Bsc4I	CCNNNNNNNGG	2 cut(s)	127, 1282
Bse1I	ACTGG	4 cut(s)	353, 1690, 2113, 2302
Bse3DI	GCAATG	3 cut(s)	732, 861, 1089
Bse8I	GATNNNNATC	1 cut(s)	399
BseBI	CCWGG	5 cut(s)	74, 122, 219, 1199, 2414
BseCI	ATCGAT	1 cut(s)	1524
BseDI	CCNNGG	2 cut(s)	218, 1197
BseGI	GGATG	7 cut(s)	82, 361, 588, 842, 1752, 1858, 1865
BseJI	GATNNNNATC	1 cut(s)	399
BseLI	CCNNNNNNNGG	2 cut(s)	127, 1282
BseMI	GCAATG	3 cut(s)	732, 861, 1089
BseMII	CTCAG	8 cut(s)	227, 589, 606, 886, 1116, 1170, 1944, 2377
BseNI	ACTGG	4 cut(s)	353, 1690, 2113, 2302
BseRI	GAGGAG	2 cut(s)	891, 1889
BseSI	GKGCMC	1 cut(s)	2381
BseXI	GCAGC	7 cut(s)	232, 1191, 1351, 1723, 1726, 1971, 2588
BsgI	GTGCAG	2 cut(s)	312, 401
BshFI	GGCC	8 cut(s)	217, 223, 258, 882, 1090, 1569, 2051, 2348
BshNI	GGYRCC	1 cut(s)	1423
BshVI	ATCGAT	1 cut(s)	1524
BsiHKAI	GWGCWC	1 cut(s)	147
BsiHKCI	CYCGRG	1 cut(s)	894
BsiSI	CCGG	1 cut(s)	543
BslFI	GGGAC	2 cut(s)	904, 2270
BslI	CCNNNNNNNGG	2 cut(s)	127, 1282
BsmAI	GTCTC	3 cut(s)	1706, 2001, 2452
BsmFI	GGGAC	2 cut(s)	904, 2270
BsmI	GAATGC	1 cut(s)	997
BsnI	GGCC	8 cut(s)	217, 223, 258, 882, 1090, 1569, 2051, 2348
BsoBI	CYCGRG	1 cut(s)	894
Bsp1286I	GDGCHC	2 cut(s)	147, 2381
Bsp1720I	GCTNAGC	1 cut(s)	1179
BspACI	CCGC	6 cut(s)	737, 1087, 1566, 1669, 2190, 2381
BspANI	GGCC	8 cut(s)	217, 223, 258, 882, 1090, 1569, 2051, 2348
BspCNI	CTCAG	8 cut(s)	228, 588, 607, 885, 1117, 1171, 1945, 2376
BspDI	ATCGAT	1 cut(s)	1524
BspHI	TCATGA	3 cut(s)	645, 1726, 2101
BspLI	GGNNCC	5 cut(s)	300, 1287, 1425, 2258, 2259
BspPI	GGATC	8 cut(s)	293, 306, 395, 1021, 1088, 1520, 1553, 2563
BspT107I	GGYRCC	1 cut(s)	1423
BsrDI	GCAATG	3 cut(s)	732, 861, 1089
BsrI	ACTGG	4 cut(s)	353, 1690, 2113, 2302
BssECI	CCNNGG	2 cut(s)	218, 1197
BssNAI	GTATAC	1 cut(s)	794
BssNI	GRCGYC	1 cut(s)	108
Bst1107I	GTATAC	1 cut(s)	794
Bst2UI	CCWGG	5 cut(s)	74, 122, 219, 1199, 2414
Bst4CI	ACNGT	4 cut(s)	272, 290, 1548, 1653
Bst6I	CTCTTC	4 cut(s)	1355, 1704, 2606, 2630
BstACI	GRCGYC	1 cut(s)	108
BstAPI	GCANNNNNTGC	1 cut(s)	1604
BstBAI	YACGTR	1 cut(s)	1190
BstC8I	GCNNGC	5 cut(s)	31, 243, 416, 1185, 2381
BstENI	CCTNNNNNAGG	1 cut(s)	1280
BstF5I	GGATG	7 cut(s)	82, 361, 588, 842, 1752, 1858, 1865
BstMAI	GTCTC	3 cut(s)	1706, 2001, 2452
BstMWI	GCNNNNNNNGC	7 cut(s)	133, 142, 458, 1184, 1213, 1604, 2519
BstNI	CCWGG	5 cut(s)	74, 122, 219, 1199, 2414
BstNSI	RCATGY	2 cut(s)	445, 2138
BstSCI	CCNGG	5 cut(s)	72, 120, 217, 1197, 2412
BstSLI	GKGCMC	1 cut(s)	2381
BstV1I	GCAGC	7 cut(s)	232, 1191, 1351, 1723, 1726, 1971, 2588
BstV2I	GAAGAC	3 cut(s)	76, 178, 1617
BstX2I	RGATCY	5 cut(s)	298, 1013, 1171, 1558, 2302
BstYI	RGATCY	5 cut(s)	298, 1013, 1171, 1558, 2302
BstZ17I	GTATAC	1 cut(s)	794
Bsu15I	ATCGAT	1 cut(s)	1524
BsuI	GTATCC	2 cut(s)	927, 1450
BsuRI	GGCC	8 cut(s)	217, 223, 258, 882, 1090, 1569, 2051, 2348
BsuTUI	ATCGAT	1 cut(s)	1524
BtgZI	GCGATG	1 cut(s)	771
BtrI	CACGTC	1 cut(s)	1604
BtsCI	GGATG	7 cut(s)	82, 361, 588, 842, 1752, 1858, 1865
BtsI	GCAGTG	1 cut(s)	2556
BtsIMutI	CAGTG	4 cut(s)	346, 1371, 2106, 2556
Cac8I	GCNNGC	5 cut(s)	31, 243, 416, 1185, 2381
CaiI	CAGNNNCTG	2 cut(s)	614, 1204
CciI	TCATGA	3 cut(s)	645, 1726, 2101
Cfr13I	GGNCC	4 cut(s)	221, 256, 2257, 2346
ClaI	ATCGAT	1 cut(s)	1524
CseI	GACGC	2 cut(s)	2284, 2528
Csp6I	GTAC	8 cut(s)	233, 273, 433, 1424, 1544, 1649, 1705, 2130
CspCI	CAANNNNNGTGG	2 cut(s)	1291, 1326
CviQI	GTAC	8 cut(s)	233, 273, 433, 1424, 1544, 1649, 1705, 2130
DraI	TTTAAA	2 cut(s)	519, 768
EaeI	YGGCCR	1 cut(s)	1567
Eam1104I	CTCTTC	4 cut(s)	1355, 1704, 2606, 2630
EarI	CTCTTC	4 cut(s)	1355, 1704, 2606, 2630
Ecl136II	GAGCTC	1 cut(s)	145
Eco147I	AGGCCT	1 cut(s)	882
Eco24I	GRGCYC	1 cut(s)	147
Eco32I	GATATC	1 cut(s)	2569
Eco47I	GGWCC	1 cut(s)	2257
Eco53kI	GAGCTC	1 cut(s)	145
Eco57I	CTGAAG	3 cut(s)	1232, 1469, 1629
Eco72I	CACGTG	1 cut(s)	1190
Eco88I	CYCGRG	1 cut(s)	894
EcoICRI	GAGCTC	1 cut(s)	145
EcoNI	CCTNNNNNAGG	1 cut(s)	1280
EcoO109I	RGGNCCY	2 cut(s)	256, 2257
EcoRII	CCWGG	5 cut(s)	72, 120, 217, 1197, 2412
EcoRV	GATATC	1 cut(s)	2569
EcoT22I	ATGCAT	1 cut(s)	2164
EcoT38I	GRGCYC	1 cut(s)	147
FalI	AAGNNNNNCTT	4 cut(s)	1158, 1190, 1398, 1430
FaqI	GGGAC	2 cut(s)	904, 2270
FauI	CCCGC	1 cut(s)	2388
FbaI	TGATCA	2 cut(s)	1626, 1840
FblI	GTMKAC	2 cut(s)	793, 2029
Fnu4HI	GCNGC	9 cut(s)	246, 1088, 1205, 1340, 1567, 1737, 1740, 1985, 2577
FokI	GGATG	7 cut(s)	89, 348, 595, 829, 1759, 1852, 1865
FriOI	GRGCYC	1 cut(s)	147
Fsp4HI	GCNGC	9 cut(s)	246, 1088, 1205, 1340, 1567, 1737, 1740, 1985, 2577
FspBI	CTAG	4 cut(s)	923, 1271, 1871, 2318
GluI	GCNGC	9 cut(s)	246, 1088, 1205, 1340, 1567, 1737, 1740, 1985, 2577
GsuI	CTGGAG	3 cut(s)	180, 2319, 2435
HaeIII	GGCC	8 cut(s)	217, 223, 258, 882, 1090, 1569, 2051, 2348
HapII	CCGG	1 cut(s)	543
HgaI	GACGC	2 cut(s)	2284, 2528
Hin1I	GRCGYC	1 cut(s)	108
HincII	GTYRAC	2 cut(s)	2095, 2478
HindII	GTYRAC	2 cut(s)	2095, 2478
HindIII	AAGCTT	1 cut(s)	1239
HinfI	GANTC	8 cut(s)	503, 666, 1060, 1777, 1879, 1939, 2372, 2433
HpaI	GTTAAC	1 cut(s)	2095
HpaII	CCGG	1 cut(s)	543
HphI	GGTGA	1 cut(s)	557
Hpy166II	GTNNAC	8 cut(s)	794, 1397, 1656, 1784, 2030, 2095, 2132, 2478
Hpy8I	GTNNAC	8 cut(s)	794, 1397, 1656, 1784, 2030, 2095, 2132, 2478
Hpy99I	CGWCG	1 cut(s)	2522
HpyCH4III	ACNGT	4 cut(s)	272, 290, 1548, 1653
HpyCH4IV	ACGT	5 cut(s)	108, 1189, 1442, 1603, 2283
HpyF10VI	GCNNNNNNNGC	7 cut(s)	133, 142, 458, 1184, 1213, 1604, 2519
HpySE526I	ACGT	5 cut(s)	108, 1189, 1442, 1603, 2283
Hsp92I	GRCGYC	1 cut(s)	108
KflI	GGGWCCC	1 cut(s)	2257
KpnI	GGTACC	1 cut(s)	1427
Ksp22I	TGATCA	2 cut(s)	1626, 1840
KspAI	GTTAAC	1 cut(s)	2095
LmnI	GCTCC	4 cut(s)	124, 466, 1201, 2195
Lsp1109I	GCAGC	7 cut(s)	232, 1191, 1351, 1723, 1726, 1971, 2588
LweI	GCATC	7 cut(s)	67, 439, 573, 851, 2149, 2207, 2500
MaeI	CTAG	4 cut(s)	923, 1271, 1871, 2318
MaeII	ACGT	5 cut(s)	108, 1189, 1442, 1603, 2283
MaeIII	GTNAC	5 cut(s)	784, 1443, 1487, 2210, 2434
MfeI	CAATTG	3 cut(s)	624, 1805, 2609
MflI	RGATCY	5 cut(s)	298, 1013, 1171, 1558, 2302
MhlI	GDGCHC	2 cut(s)	147, 2381
MlyI	GAGTC	2 cut(s)	512, 2442
MmeI	TCCRAC	4 cut(s)	428, 560, 1561, 2062
Mph1103I	ATGCAT	1 cut(s)	2164
MroXI	GAANNNNTTC	3 cut(s)	203, 1290, 2492
MslI	CAYNNNNRTG	4 cut(s)	737, 1919, 2057, 2167
MspI	CCGG	1 cut(s)	543
MspR9I	CCNGG	5 cut(s)	74, 122, 219, 1199, 2414
MunI	CAATTG	3 cut(s)	624, 1805, 2609
Mva1269I	GAATGC	1 cut(s)	997
MvaI	CCWGG	5 cut(s)	74, 122, 219, 1199, 2414
MwoI	GCNNNNNNNGC	7 cut(s)	133, 142, 458, 1184, 1213, 1604, 2519
NlaIV	GGNNCC	5 cut(s)	300, 1287, 1425, 2258, 2259
NmeAIII	GCCGAG	1 cut(s)	193
NmuCI	GTSAC	3 cut(s)	784, 1487, 2434
NsiI	ATGCAT	1 cut(s)	2164
NspI	RCATGY	2 cut(s)	445, 2138
PaeR7I	CTCGAG	1 cut(s)	894
PagI	TCATGA	3 cut(s)	645, 1726, 2101
PceI	AGGCCT	1 cut(s)	882
PciI	ACATGT	2 cut(s)	441, 2134
PcsI	WCGNNNNNNNCGW	1 cut(s)	147
PctI	GAATGC	1 cut(s)	997
PdmI	GAANNNNTTC	3 cut(s)	203, 1290, 2492
PfeI	GAWTC	6 cut(s)	666, 1060, 1777, 1879, 1939, 2372
PfoI	TCCNGGA	3 cut(s)	72, 120, 2412
PkrI	GCNGC	9 cut(s)	247, 1089, 1206, 1341, 1568, 1738, 1741, 1986, 2578
PleI	GAGTC	2 cut(s)	511, 2441
PmaCI	CACGTG	1 cut(s)	1190
PmlI	CACGTG	1 cut(s)	1190
PpsI	GAGTC	2 cut(s)	511, 2441
Ppu21I	YACGTR	1 cut(s)	1190
PpuMI	RGGWCCY	1 cut(s)	2257
PscI	ACATGT	2 cut(s)	441, 2134
Psp124BI	GAGCTC	1 cut(s)	147
Psp5II	RGGWCCY	1 cut(s)	2257
Psp6I	CCWGG	5 cut(s)	72, 120, 217, 1197, 2412
PspCI	CACGTG	1 cut(s)	1190
PspGI	CCWGG	5 cut(s)	72, 120, 217, 1197, 2412
PspN4I	GGNNCC	5 cut(s)	300, 1287, 1425, 2258, 2259
PspPI	GGNCC	4 cut(s)	221, 256, 2257, 2346
PspPPI	RGGWCCY	1 cut(s)	2257
PstNI	CAGNNNCTG	2 cut(s)	614, 1204
PsuI	RGATCY	5 cut(s)	298, 1013, 1171, 1558, 2302
RsaI	GTAC	8 cut(s)	234, 274, 434, 1425, 1545, 1650, 1706, 2131
RsaNI	GTAC	8 cut(s)	233, 273, 433, 1424, 1544, 1649, 1705, 2130
RseI	CAYNNNNRTG	4 cut(s)	737, 1919, 2057, 2167
SacI	GAGCTC	1 cut(s)	147
SatI	GCNGC	9 cut(s)	246, 1088, 1205, 1340, 1567, 1737, 1740, 1985, 2577
Sau96I	GGNCC	4 cut(s)	221, 256, 2257, 2346
ScaI	AGTACT	1 cut(s)	434
SchI	GAGTC	2 cut(s)	512, 2442
ScrFI	CCNGG	5 cut(s)	74, 122, 219, 1199, 2414
SduI	GDGCHC	2 cut(s)	147, 2381
SfaNI	GCATC	7 cut(s)	67, 439, 573, 851, 2149, 2207, 2500
Sfr274I	CTCGAG	1 cut(s)	894
SinI	GGWCC	1 cut(s)	2257
SlaI	CTCGAG	1 cut(s)	894
SmiMI	CAYNNNNRTG	4 cut(s)	737, 1919, 2057, 2167
SmlI	CTYRAG	1 cut(s)	894
SmoI	CTYRAG	1 cut(s)	894
SseBI	AGGCCT	1 cut(s)	882
SsiI	CCGC	6 cut(s)	737, 1087, 1566, 1669, 2190, 2381
SspI	AATATT	2 cut(s)	1137, 1765
SspMI	CTAG	4 cut(s)	923, 1271, 1871, 2318
SstI	GAGCTC	1 cut(s)	147
StuI	AGGCCT	1 cut(s)	882
StyD4I	CCNGG	5 cut(s)	72, 120, 217, 1197, 2412
TaaI	ACNGT	4 cut(s)	272, 290, 1548, 1653
TaiI	ACGT	5 cut(s)	111, 1192, 1445, 1606, 2286
TaqI	TCGA	6 cut(s)	699, 810, 895, 1524, 2517, 2632
TatI	WGTACW	4 cut(s)	232, 432, 1648, 2129
TauI	GCSGC	2 cut(s)	1090, 1569
TfiI	GAWTC	6 cut(s)	666, 1060, 1777, 1879, 1939, 2372
TscAI	CASTG	4 cut(s)	353, 1378, 2113, 2556
TseFI	GTSAC	3 cut(s)	784, 1487, 2434
TseI	GCWGC	7 cut(s)	245, 1204, 1339, 1736, 1739, 1984, 2576
Tsp45I	GTSAC	3 cut(s)	784, 1487, 2434
TspRI	CASTG	4 cut(s)	353, 1378, 2113, 2556
VpaK11BI	GGWCC	1 cut(s)	2257
XagI	CCTNNNNNAGG	1 cut(s)	1280
XapI	RAATTY	4 cut(s)	1046, 1970, 2147, 2501
XceI	RCATGY	2 cut(s)	445, 2138
XcmI	CCANNNNNNNNNTGG	2 cut(s)	853, 1577
XhoI	CTCGAG	1 cut(s)	894
XmiI	GTMKAC	2 cut(s)	793, 2029
XmnI	GAANNNNTTC	3 cut(s)	203, 1290, 2492
XspI	CTAG	4 cut(s)	923, 1271, 1871, 2318
ZraI	GACGTC	1 cut(s)	109
ZrmI	AGTACT	1 cut(s)	434
Zsp2I	ATGCAT	1 cut(s)	2164

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.