Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Reverse (-)

9183646 .. 9192935

Gene Structure 9290 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG080800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3159 bp

ATGGAGAGAGCAGCTCTCCTTCGCTCTTCTCTTTCCTGCACTAATAGAGCCTTCTTCTCCTTCCGCCCCCCCTCTATCCCATTCTTTCGATTCTCTCGCTCCTCCTCTTCTTCTTCTTCTGCTCTCAGAACCCATCGCCACCGTCCGATTCTCCGACCCTCCTCCGGCCGCCCCCTCCGCCGCACCAGCCTCTTACCACTCCCCGCCGCTTCCACCCACTTCGCCACGCGCTTCTCCTCCCTCTCCCCACGCGCTGTCGCCACGCCCTTAACCCCCTCGCCGTCAGAGTTCTCTGGGGTTTCCGATGAGGTTGCGGAACAGCTGGGATTCGAGAAGGTCGCCGAAGAGTTTATAGGAGAGTGTAAATCCAAAGCTCTGCTCTTTAAGCACAAAAAGACCGGTGCTCAGGTGATCTCTGTTTCCAACGACGATGAGAACAAGGTTTTCGGCATTGTTTTCCGAACACCACCAAATGATTCGACTGGAATCCCACACATTTTGGAGCACAGTGTGTTGTGTGGTTCTAGGAAATACCCTTTGAAAGAGCCGTTTGTGGAATTGTTGAAAGGGAGCTTGAATACTTTTCTCAATGCATTCACCTATCCTGATAGGACTTGCTACCCTGTTGCCTCCACAAATTCAAAGGATTTCTATAATCTGGTCGATGTGTACCTGGATGCTGTGTTCTTTCCAAAATGCGTGGAGGATTTCCAGACTTTCCAGCAGGAGGGCTGGCACTATGAGCTCAACGATCCTTCTGAAGACATATCTTACAAAGGAGTTGTTTTCAATGAGATGAAAGGGGTGTACTCCCAGCCTGATAATATATTAGGGCGGATTGCTCAACAGGCTCTTTTTCCAGACAACACCTATGGTGTGGACAGTGGAGGTGATCCAAAAGTTATTCCCAAGCTGACTTATGAGGAATTTAAGGAATTTCACCGTAAATATTACCATCCGAGCAATGCCAGGATTTGGTTCTATGGAGATGATGATCCAACTGAGCGCCTACGCATTTTAAGTGAGTACCTAGACATGTTTGATGCAAGTTCAGCTCCAAATGAATCAAGGGTTCAAACACAAAAACTATTTTCGGAACCAGTTAGGATTTCTGAGACATATCCTTCTGGTGAAGGCGGCGACTTAAAGAAAAAAAGCATGGTATGCATCAATTGGCTGCTCTCTGAGAAACCCCTGGACTTGGAAACTGAGCTTGCCCTTGGGTTTTTAGATCATCTTATGTTGGGAACTCCTGCGTCTCCATTGAGGAAAATATTGCTGGAAAGTGGCTTGGGAGAAGCAATCATTGGAGGCGGGGTTGAAGATGAGCTTCTTCAACCTCAATTTAGTATCGGGTTGAAGGGTGTTTCTCAAGATAATATTCAAAAGGTAGAAGAATTAGTCATGAGTACACTTAAAAAATTAGCAGAAGAAGGGTTCGACACAGCTGCTGTAGAAGCATCCATGAATACCATTGAGTTTTCTCTCAGGGAAAACAACACCGGATCATTTCCTCGTGGTTTGTCCTTAATGCTTCGATCCATGGGAAAGTGGATATATGATATGGATCCATTTCAGCCATTGAAGTATGAGAAACCTTTACTGGCACTAAAAGCAAGAATCGAACAGGAAGGCTCTAAAGCCGTTTTCTCTCCTTTAATAGAGAAATTTATTTTGAACAATCTTCATCGAGTTGTGGTAGAAATGCAGCCTGATCCAGAAAAAGCTTCTCGTGATGAAGCAGCTGAGAAAGAAATTTTGGAGAAAGTAAAAGCAGGTATGACGGAAGAAGATCTTGCTGAGCTAGCACGCGCTACACAAGAACTGAAACTGAAGCAGGAAACTCCTGATCCACCAGAAGCTCTGAGAAGTGTTCCAAGCCTCTCTCTGCTTGACATTCCAAAGGAACCTATTCCTGTTCCCACAGAGGTTGGGGATATCAATGGAGTTAAAGTTTTGCAGCATGACCTTTTCACAAATGATGTTCTCTACACTGAAGTTGTGTTCAATATGAGTTCACTCAAGCAAGAGCTCCTTCCTTTGGTACCTCTTTTTTGTCAATCATTGCTGGAGATGGGAACCAAAGATTTGAGTTTTGTGGAACTTAATCAGTTGATTGGAAGAAAAACTGGAGGGATATCAGTTTATCCACTGACATCATCTGTCCGGGGCAAGAAGGATCCCTGTAGCCATATAATTGTTCGAGGCAAAGCCATGGCAGGACGTGCGGCTGATCTATTTCATCTGATCAACTGTATTCTTCAAGAAGTCCAATTTACAGACCAGCAGCGGTTTAAGCAGTTTGTTTCCCAAAGCAAAGCTAGAATGGAGAACCGATTGAGAGGTAGCGGTCATGGAATTGCTGCTGCAAGGATGGATGCGAAGCTGAATGTTGCGGGGTGGATTTCTGAACAAATGGGTGGTTTCAGTTACTTGGAGTTTCTACAAGCTCTTGAAGAAAAAGTTGATAATGATTGGGAGAGAATATCTTCATCTCTTGAGGAGATTCGCAAGTCCTTACTTTCACAGGAGGGTTGCTTGATTAATATGACTGCTGAAGGAAAGAATCTTACCAACTCTGAGAAGTTTGTTGGCGAATTTCTTAATTTGCTGCCTCACAAATCTCCCCTTGCAACAACTACTTGGAATGCTCGATTACCTTCTATAAATGAAGCCATTGTTATACCTACTCAGGTTAATTATGTTGGAAAAGCAGCCAACATTTATGACACTGGGTATCAACTTAATGGAAGTGCATATGTTATCTCTAAATACATAAGTAATACATGGTTGTGGGATCGTGTACGAGTTAGTGGTGGAGCATATGGAGGTTTTTGCGACTTCGATAGTCACTCCGGTGTGTTCTCCTTCTTATCTTATCGTGACCCCAACTTGTTAAAGACACTTGATATATATGATGGAACTGGGGAATTTCTACGACAACTAGATTTGGATGAAGAAACTCTCACAAAGTCCATCATTGGAACAATTGGAGATGTTGATTCATACCAGCTTCCTGATGCCAAAGGTTATAGTAGGTATGTGGAGATAACTTCCTCTTTCTATAGAGTTGAGATTCTTATCAGCCATTATGATACAAAAGGGAACAAGAAAACAATTGTTTTATCTTTAAATATGCCTGTAGATGGAAACAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

1052

Amino Acids

117.75

Weight (kDa)

5.5

Isoelectric Point (pI)

44.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	157 - 232	3.5e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	304 - 490	3.2e-27	Peptidase M16 inactive domain
M16C_assoc	PF08367	569 - 818	2e-76	Peptidase M16C associated
PreP_C	PF22516	890 - 1017	2.3e-62	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1766
Acc65I	GGTACC	1 cut(s)	2044
AccB1I	GGYRCC	1 cut(s)	2044
AccB7I	CCANNNNNTGG	1 cut(s)	975
AccII	CGCG	3 cut(s)	229, 252, 1812
AcoI	YGGCCR	1 cut(s)	166
AcsI	RAATTY	7 cut(s)	637, 926, 935, 1667, 1755, 2597, 2930
AcuI	CTGAAG	4 cut(s)	780, 1853, 2016, 2577
AdeI	CACNNNGTG	1 cut(s)	511
AfaI	GTAC	6 cut(s)	671, 809, 1028, 1411, 2046, 2805
AfiI	CCNNNNNNNGG	6 cut(s)	164, 727, 975, 1201, 2041, 3146
AflIII	ACRYGT	1 cut(s)	1035
AgeI	ACCGGT	1 cut(s)	398
AjiI	CACGTC	1 cut(s)	2225
AjnI	CCWGG	3 cut(s)	672, 968, 1194
AjuI	GAANNNNNNNTTGG	4 cut(s)	1290, 1322, 1742, 1774
AleI	CACNNNNGTG	1 cut(s)	2856
AloI	GAACNNNNNNTCC	2 cut(s)	668, 700
Alw21I	GWGCWC	4 cut(s)	406, 507, 747, 2034
Alw26I	GTCTC	2 cut(s)	1109, 1263
AlwNI	CAGNNNCTG	1 cut(s)	1451
AoxI	GGCC	1 cut(s)	166
ApoI	RAATTY	7 cut(s)	637, 926, 935, 1667, 1755, 2597, 2930
AseI	ATTAAT	1 cut(s)	2544
AsiGI	ACCGGT	1 cut(s)	398
Asp700I	GAANNNNTTC	2 cut(s)	1911, 2488
Asp718I	GGTACC	1 cut(s)	2044
AspLEI	GCGC	4 cut(s)	231, 254, 1008, 1814
AsuC2I	CCSGG	1 cut(s)	2168
AsuHPI	GGTGA	5 cut(s)	421, 589, 902, 932, 1142
AsuNHI	GCTAGC	1 cut(s)	1804
BamHI	GGATCC	2 cut(s)	1567, 2179
BanI	GGYRCC	1 cut(s)	2044
BanII	GRGCYC	2 cut(s)	747, 2034
BauI	CACGAG	2 cut(s)	1515, 1731
BbsI	GAAGAC	1 cut(s)	768
Bbv12I	GWGCWC	4 cut(s)	406, 507, 747, 2034
BccI	CCATC	7 cut(s)	141, 963, 2068, 2368, 2912, 2984, 3140
BceAI	ACGGC	3 cut(s)	265, 532, 1628
BcgI	CGANNNNNNTGC	4 cut(s)	293, 327, 1430, 1464
BciT130I	CCWGG	3 cut(s)	674, 970, 1196
BclI	TGATCA	1 cut(s)	2247
BcnI	CCSGG	1 cut(s)	2168
BcoDI	GTCTC	2 cut(s)	1109, 1263
BfaI	CTAG	5 cut(s)	525, 1031, 1805, 2322, 2945
BfmI	CTRYAG	4 cut(s)	1452, 2185, 3064, 3141
BfoI	RGCGCY	1 cut(s)	1009
BfuAI	ACCTGC	1 cut(s)	1766
BglII	AGATCT	1 cut(s)	1792
BlpI	GCTNAGC	1 cut(s)	1800
Bme1390I	CCNGG	4 cut(s)	674, 970, 1196, 2168
BmgBI	CACGTC	1 cut(s)	2225
BmiI	GGNNCC	6 cut(s)	1098, 1569, 1908, 2046, 2080, 2181
BmrFI	CCNGG	4 cut(s)	674, 970, 1196, 2168
BmrI	ACTGGG	2 cut(s)	2742, 2934
BmsI	GCATC	6 cut(s)	667, 1033, 1176, 1469, 2368, 3010
BmtI	GCTAGC	1 cut(s)	1808
BmuI	ACTGGG	2 cut(s)	2742, 2934
BpiI	GAAGAC	1 cut(s)	768
BplI	GAGNNNNNCTC	2 cut(s)	30, 32
BpmI	CTGGAG	2 cut(s)	2090, 2151
Bpu10I	CCTNAGC	1 cut(s)	405
Bpu1102I	GCTNAGC	1 cut(s)	1800
BpuEI	CTTGAG	3 cut(s)	1356, 2006, 2519
BpuMI	CCSGG	1 cut(s)	2168
BsaBI	GATNNNNATC	3 cut(s)	993, 1566, 3080
BsaJI	CCNNGG	5 cut(s)	1194, 1219, 1542, 2167, 2214
BsaWI	WCCGGW	3 cut(s)	398, 1502, 2855
BsaXI	ACNNNNNCTCC	4 cut(s)	1302, 1332, 1938, 1968
Bsc4I	CCNNNNNNNGG	6 cut(s)	164, 727, 975, 1201, 2041, 3146
Bse118I	RCCGGY	1 cut(s)	398
Bse1I	ACTGG	6 cut(s)	487, 1100, 1608, 2134, 2737, 2929
Bse3DI	GCAATG	2 cut(s)	970, 2063
Bse8I	GATNNNNATC	3 cut(s)	993, 1566, 3080
BseBI	CCWGG	3 cut(s)	674, 970, 1196
BseDI	CCNNGG	5 cut(s)	1194, 1219, 1542, 2167, 2214
BseGI	GGATG	6 cut(s)	682, 955, 1460, 2379, 2383, 2959
BseJI	GATNNNNATC	3 cut(s)	993, 1566, 3080
BseLI	CCNNNNNNNGG	6 cut(s)	164, 727, 975, 1201, 2041, 3146
BseMI	GCAATG	2 cut(s)	970, 2063
BseNI	ACTGG	6 cut(s)	487, 1100, 1608, 2134, 2737, 2929
BseRI	GAGGAG	5 cut(s)	91, 94, 151, 226, 2516
BseX3I	CGGCCG	1 cut(s)	166
BseYI	CCCAGC	2 cut(s)	322, 813
BsgI	GTGCAG	1 cut(s)	22
Bsh1236I	CGCG	3 cut(s)	229, 252, 1812
Bsh1285I	CGRYCG	1 cut(s)	169
BshFI	GGCC	1 cut(s)	168
BshNI	GGYRCC	1 cut(s)	2044
BshTI	ACCGGT	1 cut(s)	398
BsiEI	CGRYCG	1 cut(s)	169
BsiHKAI	GWGCWC	4 cut(s)	406, 507, 747, 2034
BsiSI	CCGG	5 cut(s)	165, 399, 1503, 2167, 2856
BslI	CCNNNNNNNGG	6 cut(s)	164, 727, 975, 1201, 2041, 3146
BsmAI	GTCTC	2 cut(s)	1109, 1263
BsmBI	CGTCTC	1 cut(s)	1263
BsmI	GAATGC	2 cut(s)	593, 2653
BsnI	GGCC	1 cut(s)	168
Bsp1286I	GDGCHC	4 cut(s)	406, 507, 747, 2034
Bsp1720I	GCTNAGC	1 cut(s)	1800
Bsp19I	CCATGG	2 cut(s)	1542, 2214
BspANI	GGCC	1 cut(s)	168
BspFNI	CGCG	3 cut(s)	229, 252, 1812
BspHI	TCATGA	1 cut(s)	1404
BspLI	GGNNCC	6 cut(s)	1098, 1569, 1908, 2046, 2080, 2181
BspMI	ACCTGC	1 cut(s)	1766
BspOI	GCTAGC	1 cut(s)	1808
BspQI	GCTCTTC	1 cut(s)	31
BspT107I	GGYRCC	1 cut(s)	2044
BsrDI	GCAATG	2 cut(s)	970, 2063
BsrFI	RCCGGY	1 cut(s)	398
BsrI	ACTGG	6 cut(s)	487, 1100, 1608, 2134, 2737, 2929
BssAI	RCCGGY	1 cut(s)	398
BssECI	CCNNGG	5 cut(s)	1194, 1219, 1542, 2167, 2214
BssSI	CACGAG	2 cut(s)	1515, 1731
BssT1I	CCWWGG	3 cut(s)	1219, 1542, 2214
Bst2BI	CACGAG	2 cut(s)	1515, 1731
Bst2UI	CCWGG	3 cut(s)	674, 970, 1196
Bst4CI	ACNGT	5 cut(s)	143, 509, 884, 944, 2255
Bst6I	CTCTTC	3 cut(s)	31, 112, 339
BstAPI	GCANNNNNTGC	2 cut(s)	1164, 2225
BstC8I	GCNNGC	4 cut(s)	734, 1215, 1806, 1810
BstDSI	CCRYGG	2 cut(s)	1542, 2214
BstF5I	GGATG	6 cut(s)	682, 955, 1460, 2379, 2383, 2959
BstFNI	CGCG	3 cut(s)	229, 252, 1812
BstH2I	RGCGCY	1 cut(s)	1009
BstHHI	GCGC	4 cut(s)	231, 254, 1008, 1814
BstMAI	GTCTC	2 cut(s)	1109, 1263
BstMCI	CGRYCG	1 cut(s)	169
BstNI	CCWGG	3 cut(s)	674, 970, 1196
BstNSI	RCATGY	1 cut(s)	1039
BstSCI	CCNGG	4 cut(s)	672, 968, 1194, 2166
BstSFI	CTRYAG	4 cut(s)	1452, 2185, 3064, 3141
BstUI	CGCG	3 cut(s)	229, 252, 1812
BstV2I	GAAGAC	1 cut(s)	768
BstX2I	RGATCY	3 cut(s)	1567, 1792, 2179
BstYI	RGATCY	3 cut(s)	1567, 1792, 2179
BstZI	CGGCCG	1 cut(s)	166
BsuRI	GGCC	1 cut(s)	168
BtgI	CCRYGG	2 cut(s)	1542, 2214
BtgZI	GCGATG	1 cut(s)	119
BtrI	CACGTC	1 cut(s)	2225
BtsCI	GGATG	6 cut(s)	682, 955, 1460, 2379, 2383, 2959
BtsIMutI	CAGTG	5 cut(s)	514, 889, 1992, 2150, 2730
BveI	ACCTGC	1 cut(s)	1766
Cac8I	GCNNGC	4 cut(s)	734, 1215, 1806, 1810
CaiI	CAGNNNCTG	1 cut(s)	1451
CciI	TCATGA	1 cut(s)	1404
CfoI	GCGC	4 cut(s)	231, 254, 1008, 1814
Cfr10I	RCCGGY	1 cut(s)	398
CseI	GACGC	1 cut(s)	1245
Csp6I	GTAC	6 cut(s)	670, 808, 1027, 1410, 2045, 2804
CspAI	ACCGGT	1 cut(s)	398
CspCI	CAANNNNNGTGG	6 cut(s)	480, 515, 681, 716, 1912, 1947
CviAII	CATG	9 cut(s)	1036, 1159, 1405, 1465, 1543, 1964, 2215, 2354, 2787
CviQI	GTAC	6 cut(s)	670, 808, 1027, 1410, 2045, 2804
DraI	TTTAAA	1 cut(s)	3132
DraIII	CACNNNGTG	1 cut(s)	511
EaeI	YGGCCR	1 cut(s)	166
EagI	CGGCCG	1 cut(s)	166
Eam1104I	CTCTTC	3 cut(s)	31, 112, 339
EarI	CTCTTC	3 cut(s)	31, 112, 339
EciI	GGCGGA	3 cut(s)	53, 167, 850
Ecl136II	GAGCTC	2 cut(s)	745, 2032
EclXI	CGGCCG	1 cut(s)	166
Eco130I	CCWWGG	3 cut(s)	1219, 1542, 2214
Eco24I	GRGCYC	2 cut(s)	747, 2034
Eco32I	GATATC	2 cut(s)	1939, 2139
Eco52I	CGGCCG	1 cut(s)	166
Eco53kI	GAGCTC	2 cut(s)	745, 2032
Eco57I	CTGAAG	4 cut(s)	780, 1853, 2016, 2577
EcoICRI	GAGCTC	2 cut(s)	745, 2032
EcoRII	CCWGG	3 cut(s)	672, 968, 1194
EcoRV	GATATC	2 cut(s)	1939, 2139
EcoT14I	CCWWGG	3 cut(s)	1219, 1542, 2214
EcoT22I	ATGCAT	2 cut(s)	595, 1169
EcoT38I	GRGCYC	2 cut(s)	747, 2034
ErhI	CCWWGG	3 cut(s)	1219, 1542, 2214
Esp3I	CGTCTC	1 cut(s)	1263
FaeI	CATG	9 cut(s)	1039, 1162, 1408, 1468, 1546, 1967, 2218, 2357, 2790
FalI	AAGNNNNNCTT	6 cut(s)	1314, 1346, 1779, 1811, 2019, 2051
FatI	CATG	9 cut(s)	1035, 1158, 1404, 1464, 1542, 1963, 2214, 2353, 2786
FauI	CCCGC	3 cut(s)	211, 1307, 2389
FauNDI	CATATG	2 cut(s)	2758, 2824
FbaI	TGATCA	1 cut(s)	2247
FokI	GGATG	6 cut(s)	689, 942, 1447, 2386, 2390, 2966
FriOI	GRGCYC	2 cut(s)	747, 2034
FspBI	CTAG	5 cut(s)	525, 1031, 1805, 2322, 2945
GlaI	GCGC	4 cut(s)	230, 253, 1007, 1813
GsaI	CCCAGC	2 cut(s)	326, 817
GsuI	CTGGAG	2 cut(s)	2090, 2151
HaeII	RGCGCY	1 cut(s)	1009
HaeIII	GGCC	1 cut(s)	168
HapII	CCGG	5 cut(s)	165, 399, 1503, 2167, 2856
HgaI	GACGC	1 cut(s)	1245
HhaI	GCGC	4 cut(s)	231, 254, 1008, 1814
Hin1II	CATG	9 cut(s)	1039, 1162, 1408, 1468, 1546, 1967, 2218, 2357, 2790
Hin6I	GCGC	4 cut(s)	229, 252, 1006, 1812
HinP1I	GCGC	4 cut(s)	229, 252, 1006, 1812
HindIII	AAGCTT	1 cut(s)	1725
HpaII	CCGG	5 cut(s)	165, 399, 1503, 2167, 2856
HphI	GGTGA	5 cut(s)	421, 589, 902, 932, 1142
Hpy166II	GTNNAC	6 cut(s)	670, 808, 880, 1412, 2018, 2804
Hpy8I	GTNNAC	6 cut(s)	670, 808, 880, 1412, 2018, 2804
Hpy99I	CGWCG	1 cut(s)	431
HpyCH4III	ACNGT	5 cut(s)	143, 509, 884, 944, 2255
HpyCH4IV	ACGT	1 cut(s)	2224
HpyCH4V	TGCA	9 cut(s)	39, 593, 1046, 1167, 1708, 1960, 2369, 2633, 2756
HpySE526I	ACGT	1 cut(s)	2224
Hsp92II	CATG	9 cut(s)	1039, 1162, 1408, 1468, 1546, 1967, 2218, 2357, 2790
HspAI	GCGC	4 cut(s)	229, 252, 1006, 1812
KpnI	GGTACC	1 cut(s)	2048
Ksp22I	TGATCA	1 cut(s)	2247
LguI	GCTCTTC	1 cut(s)	31
LmnI	GCTCC	6 cut(s)	104, 502, 570, 1060, 2037, 2819
LweI	GCATC	6 cut(s)	667, 1033, 1176, 1469, 2368, 3010
MaeI	CTAG	5 cut(s)	525, 1031, 1805, 2322, 2945
MaeII	ACGT	1 cut(s)	2224
MaeIII	GTNAC	3 cut(s)	2429, 2849, 2882
MfeI	CAATTG	3 cut(s)	1171, 2988, 3117
MflI	RGATCY	3 cut(s)	1567, 1792, 2179
MhlI	GDGCHC	4 cut(s)	406, 507, 747, 2034
MmeI	TCCRAC	4 cut(s)	178, 447, 1022, 2686
Mph1103I	ATGCAT	2 cut(s)	595, 1169
MroXI	GAANNNNTTC	2 cut(s)	1911, 2488
MslI	CAYNNNNRTG	2 cut(s)	2791, 2856
MspA1I	CMGCKG	4 cut(s)	322, 1448, 1745, 2290
MspI	CCGG	5 cut(s)	165, 399, 1503, 2167, 2856
MspR9I	CCNGG	4 cut(s)	674, 970, 1196, 2168
MunI	CAATTG	3 cut(s)	1171, 2988, 3117
Mva1269I	GAATGC	2 cut(s)	593, 2653
MvaI	CCWGG	3 cut(s)	674, 970, 1196
MvnI	CGCG	3 cut(s)	229, 252, 1812
NciI	CCSGG	1 cut(s)	2168
NcoI	CCATGG	2 cut(s)	1542, 2214
NdeI	CATATG	2 cut(s)	2758, 2824
NheI	GCTAGC	1 cut(s)	1804
NlaIII	CATG	9 cut(s)	1039, 1162, 1408, 1468, 1546, 1967, 2218, 2357, 2790
NlaIV	GGNNCC	6 cut(s)	1098, 1569, 1908, 2046, 2080, 2181
NmuCI	GTSAC	2 cut(s)	2849, 2882
NsiI	ATGCAT	2 cut(s)	595, 1169
NspI	RCATGY	1 cut(s)	1039
OliI	CACNNNNGTG	1 cut(s)	2856
PagI	TCATGA	1 cut(s)	1404
PciI	ACATGT	1 cut(s)	1035
PciSI	GCTCTTC	1 cut(s)	31
PctI	GAATGC	2 cut(s)	593, 2653
PdmI	GAANNNNTTC	2 cut(s)	1911, 2488
PflMI	CCANNNNNTGG	1 cut(s)	975
PinAI	ACCGGT	1 cut(s)	398
PscI	ACATGT	1 cut(s)	1035
PshBI	ATTAAT	1 cut(s)	2544
Psp124BI	GAGCTC	2 cut(s)	747, 2034
Psp6I	CCWGG	3 cut(s)	672, 968, 1194
PspFI	CCCAGC	2 cut(s)	322, 813
PspGI	CCWGG	3 cut(s)	672, 968, 1194
PspN4I	GGNNCC	6 cut(s)	1098, 1569, 1908, 2046, 2080, 2181
PstNI	CAGNNNCTG	1 cut(s)	1451
PsuI	RGATCY	3 cut(s)	1567, 1792, 2179
PvuII	CAGCTG	3 cut(s)	322, 1448, 1745
RsaI	GTAC	6 cut(s)	671, 809, 1028, 1411, 2046, 2805
RsaNI	GTAC	6 cut(s)	670, 808, 1027, 1410, 2045, 2804
RseI	CAYNNNNRTG	2 cut(s)	2791, 2856
SacI	GAGCTC	2 cut(s)	747, 2034
SapI	GCTCTTC	1 cut(s)	31
ScrFI	CCNGG	4 cut(s)	674, 970, 1196, 2168
SduI	GDGCHC	4 cut(s)	406, 507, 747, 2034
SfaNI	GCATC	6 cut(s)	667, 1033, 1176, 1469, 2368, 3010
SfcI	CTRYAG	4 cut(s)	1452, 2185, 3064, 3141
SmiMI	CAYNNNNRTG	2 cut(s)	2791, 2856
SmlI	CTYRAG	3 cut(s)	1371, 2021, 2498
SmoI	CTYRAG	3 cut(s)	1371, 2021, 2498
SspI	AATATT	3 cut(s)	950, 1275, 1381
SspMI	CTAG	5 cut(s)	525, 1031, 1805, 2322, 2945
SstI	GAGCTC	2 cut(s)	747, 2034
StyD4I	CCNGG	4 cut(s)	672, 968, 1194, 2166
StyI	CCWWGG	3 cut(s)	1219, 1542, 2214
TaaI	ACNGT	5 cut(s)	143, 509, 884, 944, 2255
TaiI	ACGT	1 cut(s)	2227
TatI	WGTACW	2 cut(s)	807, 1409
TauI	GCSGC	5 cut(s)	171, 183, 209, 1140, 2231
TscAI	CASTG	5 cut(s)	514, 889, 1999, 2157, 2737
TseFI	GTSAC	2 cut(s)	2849, 2882
Tsp45I	GTSAC	2 cut(s)	2849, 2882
TspGWI	ACGGA	1 cut(s)	1799
TspRI	CASTG	5 cut(s)	514, 889, 1999, 2157, 2737
Van91I	CCANNNNNTGG	1 cut(s)	975
VspI	ATTAAT	1 cut(s)	2544
XapI	RAATTY	7 cut(s)	637, 926, 935, 1667, 1755, 2597, 2930
XceI	RCATGY	1 cut(s)	1039
XmnI	GAANNNNTTC	2 cut(s)	1911, 2488
XspI	CTAG	5 cut(s)	525, 1031, 1805, 2322, 2945
Zsp2I	ATGCAT	2 cut(s)	595, 1169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.