Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Reverse (-)

14323652 .. 14330088

Gene Structure 6437 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1150900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGGAGGGAGCAACTCTGCTTCGCTCTTCTCTCGCCTCCAGCAACCGCCTCTTCTTCTCCTTCCGCTCTTCCCGCCGCTTCGCCCTCCCCTCATCTCGCTCTTCTTCTTCCACAGCTTCTGCTGTAAGAAACCACCGCCACCGTCCGATTCTCAACCCCCGCAGACGCTCCCTGCTCCACCGCGCCAGCAGATTACTCCCCTCCTCTGCCCCCAACGTCGCTAGAAGCTTCTCCTCCCTCTCCCCGCGTGCAGTCGCCACCCCCTTCACTCAGTCCCCTTCAGAGTTTTCTGGGGTGCAAGATGAAGTCGTGGAAAAGCTCGGGTTCGAGAAGGTCTCTGAGGAGTTCATTGGAGAGTGTAAATCCAAAGCTTTGCTTTTCAGGCACAAAAAGACCGGTGCCCAAGTCATTTCGGTGTCCAACGACGACGAGAATAAGGTTTTCGGCATTGTTTTTCGGACCCCTCCGAATGATTCCACTGGGATCCCTCACATTCTGGAACACAGTGTGCTGTGTGGATCAAGAAAGTACCCTTTGAAAGAACCATTTGTTGAATTGTTGAAAGGGAGCTTGAATACGTTTCTGAATGCATTCACATATCCGGATAGGACTTGCTACCCAGTTGCTTCCACAAATACAAAGGATTTCTATAACCTTGTCGATGTATATCTGGATGCCGTCTTCTTTCCTAAATGTGTGGAGGACTTCCGGACTTTCCAACAGGAGGGTTGGCATTACGAGCTCAATGATCCTTCTGAAGATATATCTTATAAAGGAGTTGTTTTTAATGAGATGAAAGGGGTCTATTCCCAGCCCGATAATATACTTGGTCGGACTGCTCAACAGGCTCTTTTTCCGGACAATACCTATGGTGTTGACAGTGGAGGTGATCCAACCGTTATTCCTAAACTGACATTTGATGAGTTCAAGGAGTTCCACCGTAAATATTACCATCCCAGCAATGCCAGGATCTGGTTTTATGGAGATGATGATCCAACGGAACGCCTGCGCATTTTGAGTGAGTACCTAGATATGTTCGATGCAAGTTCATCTCCAAATGAATCAAGAATTCAACCGCAAAAACTATTTTCCAAACCAATTTGGATTTCTGGGAAATATCCTGCTGGTGAAGGTGGTGACTTAAGGAAGAAAAACATGGTATGCCTCAATTGGTTGCTCGCTGAGAATCCCCTAGACTTGGAGACTGAGCTTACGCTTGGGTTTCTGGATCATCTTATGTTGGGAACGCCTGCTTCTCCATTGAGGAAAATATTGCTGGAGAGTGGCTTGGGAGAGGCCATAGTTGGTGGTGGAGTTGAAGATGAGCTCCTACAACCCCAATTTAGTATTGGGTTGAAGGGCGTTTCGGAAGGTGACATTCAAAAGGTAGAAGAAGTAGTCATGAGTACACTTAAAAAGTTGGCAGAGGAAGGTTTTGATACAGAAGCTGTAGAAGCATCCATGAATACGATTGAGTTCTCTCTGAGGGAAAACAACACAGGATCATTTCCTCGTGGTTTGTCACTAATGCTCCGGTCCATGGGTAAATGGATATATGATATGGATCCATTTGGGCCATTAAAGTATGAGAAACCTTTACTGGCACTGAAAGCCAGGATAGAAGCTGAAGGCTCGAAGGCTGTTTTCTCTCCCTTAATAGAGAAATTTATTTTGAACAATCCCCATAGAGTTGTGGTAGAAATGCAGCCTGATCCAGAGAAAGCTTCTCGTGATGAAGAGTCTGAGAAAGAAATCTTGCAAAAAGTTAAATCAGGTATGACAGAAGAAGATCTTGCTGAGCTAGCTCGTGCTACACACGAGCTGCGACTGAGGCAGGAAACTCCTGACCCTCCGGAAGCTTTGAGAAGTGTTCCAAGTCTCTCTCTGCAAGACATTCCAAAGGAACCTACTCGTGTCCCCACACAGGTTGGGGATATCAATGGTGTGAAAGTTCTGCAGCATGACCTCTTTACAAATGATGTCCTGTACACTGAAGTTGTGTTCAATATGAGTTCACTAAAGCAAGAGCTTCTTCCTTTGGTACCTCTTTTTTGCCAATCATTGTTAGAGATGGGCACAAAAGACTTAAGTTTTGTGCAACTTAATCAGTTGATTGGAAGGAAAACTGGAGGAATATCAGTTTATCCAATGACATCATCTGTACGGGGCAAGGAGGAACCCTGTAGCCATATAATTGTTCGAGGAAAAGCCATGGCAGGACGTGCTGATGACCTATTTCAGCTGGTATACTTTGTTCCTTTTCTTTTTAGAGTTGGCCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

761

Amino Acids

85.29

Weight (kDa)

5.78

Isoelectric Point (pI)

51.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	156 - 231	2.2e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	303 - 489	4.2e-26	Peptidase M16 inactive domain
M16C_assoc	PF08367	568 - 749	1.5e-51	Peptidase M16C associated

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	771
Acc16I	TGCGCA	1 cut(s)	1010
Acc65I	GGTACC	1 cut(s)	2041
AccB1I	GGYRCC	2 cut(s)	398, 2041
AccB7I	CCANNNNNTGG	1 cut(s)	972
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	1 cut(s)	2247
AccII	CGCG	2 cut(s)	183, 247
AccIII	TCCGGA	4 cut(s)	601, 708, 856, 1852
AciI	CCGC	9 cut(s)	46, 64, 73, 76, 136, 160, 181, 245, 1076
AcoI	YGGCCR	1 cut(s)	2275
AcsI	RAATTY	2 cut(s)	1068, 1664
AcuI	CTGAAG	4 cut(s)	264, 777, 1647, 2013
AdeI	CACNNNGTG	1 cut(s)	508
AfaI	GTAC	6 cut(s)	530, 1025, 1408, 1988, 2043, 2163
AfiI	CCNNNNNNNGG	5 cut(s)	724, 972, 1198, 1540, 1924
AflII	CTTAAG	2 cut(s)	1141, 2086
AgeI	ACCGGT	1 cut(s)	395
AjiI	CACGTC	1 cut(s)	2222
AjnI	CCWGG	2 cut(s)	965, 1613
AjuI	GAANNNNNNNTTGG	2 cut(s)	1090, 1122
Alw21I	GWGCWC	2 cut(s)	744, 1329
Alw26I	GTCTC	3 cut(s)	340, 1196, 1883
AlwNI	CAGNNNCTG	3 cut(s)	119, 972, 1448
Ama87I	CYCGRG	1 cut(s)	320
Aor13HI	TCCGGA	4 cut(s)	601, 708, 856, 1852
AoxI	GGCC	3 cut(s)	1296, 1574, 2275
ApeKI	GCWGC	3 cut(s)	1705, 1822, 1957
ApoI	RAATTY	2 cut(s)	1068, 1664
ArsI	GACNNNNNNTTYG	2 cut(s)	2075, 2107
AsiGI	ACCGGT	1 cut(s)	395
Asp700I	GAANNNNTTC	1 cut(s)	590
Asp718I	GGTACC	1 cut(s)	2041
AspLEI	GCGC	2 cut(s)	185, 1011
AspS9I	GGNCC	3 cut(s)	459, 1536, 1574
AsuHPI	GGTGA	4 cut(s)	899, 1139, 1148, 1385
AsuNHI	GCTAGC	1 cut(s)	1801
AvaI	CYCGRG	1 cut(s)	320
AvaII	GGWCC	2 cut(s)	459, 1536
BaeGI	GKGCMC	2 cut(s)	403, 2078
BalI	TGGCCA	1 cut(s)	2277
BamHI	GGATCC	2 cut(s)	483, 1564
BanI	GGYRCC	2 cut(s)	398, 2041
BanII	GRGCYC	2 cut(s)	744, 1329
BauI	CACGAG	5 cut(s)	1512, 1728, 1806, 1817, 1911
BbsI	GAAGAC	1 cut(s)	673
Bbv12I	GWGCWC	2 cut(s)	744, 1329
BbvI	GCAGC	3 cut(s)	1717, 1809, 1969
BccI	CCATC	2 cut(s)	960, 2065
BceAI	ACGGC	1 cut(s)	662
BciT130I	CCWGG	2 cut(s)	967, 1615
BcoDI	GTCTC	3 cut(s)	340, 1196, 1883
BfaI	CTAG	4 cut(s)	222, 1028, 1193, 1802
BfmI	CTRYAG	3 cut(s)	1449, 1955, 2182
BfrI	CTTAAG	2 cut(s)	1141, 2086
BglII	AGATCT	1 cut(s)	1789
BisI	GCNGC	4 cut(s)	76, 1706, 1823, 1958
BlpI	GCTNAGC	1 cut(s)	1797
BlsI	GCNGC	4 cut(s)	77, 1707, 1824, 1959
Bme1390I	CCNGG	2 cut(s)	967, 1615
Bme18I	GGWCC	2 cut(s)	459, 1536
BmeT110I	CYCGRG	1 cut(s)	320
BmgBI	CACGTC	1 cut(s)	2222
BmgT120I	GGNCC	3 cut(s)	459, 1536, 1574
BmiI	GGNNCC	7 cut(s)	400, 461, 485, 1566, 1905, 2043, 2178
BmrFI	CCNGG	2 cut(s)	967, 1615
BmrI	ACTGGG	2 cut(s)	489, 614
BmsI	GCATC	3 cut(s)	664, 1030, 1466
BmtI	GCTAGC	1 cut(s)	1805
BmuI	ACTGGG	2 cut(s)	489, 614
BpiI	GAAGAC	1 cut(s)	673
BplI	GAGNNNNNCTC	2 cut(s)	320, 352
BpmI	CTGGAG	3 cut(s)	22, 1298, 2148
Bpu1102I	GCTNAGC	1 cut(s)	1797
BsaBI	GATNNNNATC	3 cut(s)	666, 990, 1563
BsaI	GGTCTC	1 cut(s)	340
BsaJI	CCNNGG	2 cut(s)	1539, 2211
BsaWI	WCCGGW	6 cut(s)	395, 601, 708, 856, 1533, 1852
BsaXI	ACNNNNNCTCC	2 cut(s)	1515, 1545
Bsc4I	CCNNNNNNNGG	5 cut(s)	724, 972, 1198, 1540, 1924
Bse118I	RCCGGY	1 cut(s)	395
Bse1I	ACTGG	4 cut(s)	484, 620, 1605, 2131
Bse3DI	GCAATG	1 cut(s)	967
Bse8I	GATNNNNATC	3 cut(s)	666, 990, 1563
BseAI	TCCGGA	4 cut(s)	601, 708, 856, 1852
BseBI	CCWGG	2 cut(s)	967, 1615
BseDI	CCNNGG	2 cut(s)	1539, 2211
BseGI	GGATG	3 cut(s)	679, 952, 1457
BseJI	GATNNNNATC	3 cut(s)	666, 990, 1563
BseLI	CCNNNNNNNGG	5 cut(s)	724, 972, 1198, 1540, 1924
BseMI	GCAATG	1 cut(s)	967
BseMII	CTCAG	8 cut(s)	284, 330, 1173, 1197, 1475, 1734, 1788, 1820
BseNI	ACTGG	4 cut(s)	484, 620, 1605, 2131
BseRI	GAGGAG	3 cut(s)	193, 223, 356
BseSI	GKGCMC	2 cut(s)	403, 2078
BseXI	GCAGC	3 cut(s)	1717, 1809, 1969
BseYI	CCCAGC	2 cut(s)	810, 956
BsgI	GTGCAG	1 cut(s)	270
Bsh1236I	CGCG	2 cut(s)	183, 247
BshFI	GGCC	3 cut(s)	1298, 1576, 2277
BshNI	GGYRCC	2 cut(s)	398, 2041
BshTI	ACCGGT	1 cut(s)	395
BsiHKAI	GWGCWC	2 cut(s)	744, 1329
BsiHKCI	CYCGRG	1 cut(s)	320
BsiSI	CCGG	6 cut(s)	396, 602, 709, 857, 1534, 1853
BslFI	GGGAC	2 cut(s)	259, 1901
BslI	CCNNNNNNNGG	5 cut(s)	724, 972, 1198, 1540, 1924
BsmAI	GTCTC	3 cut(s)	340, 1196, 1883
BsmFI	GGGAC	2 cut(s)	259, 1901
BsmI	GAATGC	2 cut(s)	590, 592
BsnI	GGCC	3 cut(s)	1298, 1576, 2277
Bso31I	GGTCTC	1 cut(s)	340
BsoBI	CYCGRG	1 cut(s)	320
Bsp1286I	GDGCHC	4 cut(s)	403, 744, 1329, 2078
Bsp13I	TCCGGA	4 cut(s)	601, 708, 856, 1852
Bsp1407I	TGTACA	1 cut(s)	1986
Bsp1720I	GCTNAGC	1 cut(s)	1797
Bsp19I	CCATGG	2 cut(s)	1539, 2211
BspACI	CCGC	9 cut(s)	46, 64, 73, 76, 136, 160, 181, 245, 1076
BspANI	GGCC	3 cut(s)	1298, 1576, 2277
BspCNI	CTCAG	8 cut(s)	283, 331, 1174, 1198, 1476, 1735, 1789, 1821
BspEI	TCCGGA	4 cut(s)	601, 708, 856, 1852
BspFNI	CGCG	2 cut(s)	183, 247
BspHI	TCATGA	1 cut(s)	1401
BspLI	GGNNCC	7 cut(s)	400, 461, 485, 1566, 1905, 2043, 2178
BspMAI	CTGCAG	1 cut(s)	1959
BspOI	GCTAGC	1 cut(s)	1805
BspQI	GCTCTTC	3 cut(s)	31, 73, 106
BspT107I	GGYRCC	2 cut(s)	398, 2041
BspTI	CTTAAG	2 cut(s)	1141, 2086
BspTNI	GGTCTC	1 cut(s)	340
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	1 cut(s)	967
BsrFI	RCCGGY	1 cut(s)	395
BsrGI	TGTACA	1 cut(s)	1986
BsrI	ACTGG	4 cut(s)	484, 620, 1605, 2131
BssAI	RCCGGY	1 cut(s)	395
BssECI	CCNNGG	2 cut(s)	1539, 2211
BssNAI	GTATAC	1 cut(s)	2248
BssSI	CACGAG	5 cut(s)	1512, 1728, 1806, 1817, 1911
BssT1I	CCWWGG	2 cut(s)	1539, 2211
Bst1107I	GTATAC	1 cut(s)	2248
Bst2BI	CACGAG	5 cut(s)	1512, 1728, 1806, 1817, 1911
Bst2UI	CCWGG	2 cut(s)	967, 1615
Bst4CI	ACNGT	5 cut(s)	143, 506, 881, 898, 941
Bst6I	CTCTTC	5 cut(s)	31, 56, 73, 106, 1731
BstAFI	CTTAAG	2 cut(s)	1141, 2086
BstAPI	GCANNNNNTGC	1 cut(s)	2222
BstAUI	TGTACA	1 cut(s)	1986
BstC8I	GCNNGC	6 cut(s)	187, 249, 1007, 1179, 1251, 1803
BstDEI	CTNAG	8 cut(s)	270, 339, 1182, 1206, 1484, 1743, 1797, 1829
BstDSI	CCRYGG	2 cut(s)	1539, 2211
BstF5I	GGATG	3 cut(s)	679, 952, 1457
BstFNI	CGCG	2 cut(s)	183, 247
BstHHI	GCGC	2 cut(s)	185, 1011
BstMAI	GTCTC	3 cut(s)	340, 1196, 1883
BstMWI	GCNNNNNNNGC	9 cut(s)	72, 382, 739, 845, 1454, 1610, 1802, 1831, 2222
BstNI	CCWGG	2 cut(s)	967, 1615
BstSCI	CCNGG	2 cut(s)	965, 1613
BstSFI	CTRYAG	3 cut(s)	1449, 1955, 2182
BstSLI	GKGCMC	2 cut(s)	403, 2078
BstUI	CGCG	2 cut(s)	183, 247
BstV1I	GCAGC	3 cut(s)	1717, 1809, 1969
BstV2I	GAAGAC	1 cut(s)	673
BstX2I	RGATCY	4 cut(s)	483, 969, 1564, 1789
BstYI	RGATCY	4 cut(s)	483, 969, 1564, 1789
BstZ17I	GTATAC	1 cut(s)	2248
BsuRI	GGCC	3 cut(s)	1298, 1576, 2277
BtgI	CCRYGG	2 cut(s)	1539, 2211
BtrI	CACGTC	1 cut(s)	2222
BtsCI	GGATG	3 cut(s)	679, 952, 1457
BtsIMutI	CAGTG	5 cut(s)	477, 511, 886, 1604, 1989
Cac8I	GCNNGC	6 cut(s)	187, 249, 1007, 1179, 1251, 1803
CaiI	CAGNNNCTG	3 cut(s)	119, 972, 1448
CciI	TCATGA	1 cut(s)	1401
CfoI	GCGC	2 cut(s)	185, 1011
Cfr10I	RCCGGY	1 cut(s)	395
Cfr13I	GGNCC	3 cut(s)	459, 1536, 1574
CseI	GACGC	1 cut(s)	174
Csp6I	GTAC	6 cut(s)	529, 1024, 1407, 1987, 2042, 2162
CspAI	ACCGGT	1 cut(s)	395
CspCI	CAANNNNNGTGG	2 cut(s)	1909, 1944
CviAII	CATG	6 cut(s)	1156, 1402, 1462, 1540, 1961, 2212
CviQI	GTAC	6 cut(s)	529, 1024, 1407, 1987, 2042, 2162
DdeI	CTNAG	8 cut(s)	270, 339, 1182, 1206, 1484, 1743, 1797, 1829
DraIII	CACNNNGTG	1 cut(s)	508
EaeI	YGGCCR	1 cut(s)	2275
Eam1104I	CTCTTC	5 cut(s)	31, 56, 73, 106, 1731
EarI	CTCTTC	5 cut(s)	31, 56, 73, 106, 1731
Ecl136II	GAGCTC	2 cut(s)	742, 1327
Eco130I	CCWWGG	2 cut(s)	1539, 2211
Eco24I	GRGCYC	2 cut(s)	744, 1329
Eco31I	GGTCTC	1 cut(s)	340
Eco32I	GATATC	1 cut(s)	1936
Eco47I	GGWCC	2 cut(s)	459, 1536
Eco53kI	GAGCTC	2 cut(s)	742, 1327
Eco57I	CTGAAG	4 cut(s)	264, 777, 1647, 2013
Eco88I	CYCGRG	1 cut(s)	320
EcoICRI	GAGCTC	2 cut(s)	742, 1327
EcoRI	GAATTC	1 cut(s)	1068
EcoRII	CCWGG	2 cut(s)	965, 1613
EcoRV	GATATC	1 cut(s)	1936
EcoT14I	CCWWGG	2 cut(s)	1539, 2211
EcoT22I	ATGCAT	1 cut(s)	592
EcoT38I	GRGCYC	2 cut(s)	744, 1329
ErhI	CCWWGG	2 cut(s)	1539, 2211
FaeI	CATG	6 cut(s)	1159, 1405, 1465, 1543, 1964, 2215
FalI	AAGNNNNNCTT	6 cut(s)	360, 392, 1776, 1808, 2016, 2048
FaqI	GGGAC	2 cut(s)	259, 1901
FatI	CATG	6 cut(s)	1155, 1401, 1461, 1539, 1960, 2211
FauI	CCCGC	3 cut(s)	80, 167, 252
FblI	GTMKAC	1 cut(s)	2247
Fnu4HI	GCNGC	4 cut(s)	76, 1706, 1823, 1958
FokI	GGATG	3 cut(s)	686, 939, 1444
FriOI	GRGCYC	2 cut(s)	744, 1329
Fsp4HI	GCNGC	4 cut(s)	76, 1706, 1823, 1958
FspBI	CTAG	4 cut(s)	222, 1028, 1193, 1802
FspI	TGCGCA	1 cut(s)	1010
GlaI	GCGC	2 cut(s)	184, 1010
GluI	GCNGC	4 cut(s)	76, 1706, 1823, 1958
GsaI	CCCAGC	2 cut(s)	814, 960
GsuI	CTGGAG	3 cut(s)	22, 1298, 2148
HaeIII	GGCC	3 cut(s)	1298, 1576, 2277
HapII	CCGG	6 cut(s)	396, 602, 709, 857, 1534, 1853
HgaI	GACGC	1 cut(s)	174
HhaI	GCGC	2 cut(s)	185, 1011
Hin1II	CATG	6 cut(s)	1159, 1405, 1465, 1543, 1964, 2215
Hin6I	GCGC	2 cut(s)	183, 1009
HinP1I	GCGC	2 cut(s)	183, 1009
HincII	GTYRAC	1 cut(s)	877
HindII	GTYRAC	1 cut(s)	877
HindIII	AAGCTT	4 cut(s)	226, 369, 1722, 1857
HinfI	GANTC	5 cut(s)	148, 473, 1061, 1186, 1739
HpaII	CCGG	6 cut(s)	396, 602, 709, 857, 1534, 1853
HphI	GGTGA	4 cut(s)	899, 1139, 1148, 1385
Hpy166II	GTNNAC	5 cut(s)	877, 1409, 1989, 2015, 2248
Hpy8I	GTNNAC	5 cut(s)	877, 1409, 1989, 2015, 2248
Hpy99I	CGWCG	3 cut(s)	221, 428, 431
HpyCH4III	ACNGT	5 cut(s)	143, 506, 881, 898, 941
HpyCH4IV	ACGT	3 cut(s)	216, 578, 2221
HpyCH4V	TGCA	9 cut(s)	251, 298, 590, 1043, 1705, 1759, 1888, 1957, 2098
HpyF10VI	GCNNNNNNNGC	9 cut(s)	72, 382, 739, 845, 1454, 1610, 1802, 1831, 2222
HpyF3I	CTNAG	8 cut(s)	270, 339, 1182, 1206, 1484, 1743, 1797, 1829
HpySE526I	ACGT	3 cut(s)	216, 578, 2221
Hsp92II	CATG	6 cut(s)	1159, 1405, 1465, 1543, 1964, 2215
HspAI	GCGC	2 cut(s)	183, 1009
Kpn2I	TCCGGA	4 cut(s)	601, 708, 856, 1852
KpnI	GGTACC	1 cut(s)	2045
LguI	GCTCTTC	3 cut(s)	31, 73, 106
LmnI	GCTCC	6 cut(s)	8, 173, 180, 567, 1332, 1536
Lsp1109I	GCAGC	3 cut(s)	1717, 1809, 1969
LweI	GCATC	3 cut(s)	664, 1030, 1466
MaeI	CTAG	4 cut(s)	222, 1028, 1193, 1802
MaeII	ACGT	3 cut(s)	216, 578, 2221
MaeIII	GTNAC	3 cut(s)	1136, 1373, 1521
MbiI	CCGCTC	1 cut(s)	66
MfeI	CAATTG	1 cut(s)	1168
MflI	RGATCY	4 cut(s)	483, 969, 1564, 1789
MhlI	GDGCHC	4 cut(s)	403, 744, 1329, 2078
MlsI	TGGCCA	1 cut(s)	2277
MluCI	AATT	7 cut(s)	554, 1068, 1098, 1168, 1340, 1664, 2193
MluNI	TGGCCA	1 cut(s)	2277
MlyI	GAGTC	1 cut(s)	1748
MmeI	TCCRAC	5 cut(s)	444, 742, 812, 917, 1019
Mox20I	TGGCCA	1 cut(s)	2277
Mph1103I	ATGCAT	1 cut(s)	592
MroI	TCCGGA	4 cut(s)	601, 708, 856, 1852
MroXI	GAANNNNTTC	1 cut(s)	590
MscI	TGGCCA	1 cut(s)	2277
MseI	TTAA	8 cut(s)	786, 1142, 1413, 1580, 1655, 1767, 2087, 2103
MslI	CAYNNNNRTG	1 cut(s)	413
Msp20I	TGGCCA	1 cut(s)	2277
MspA1I	CMGCKG	1 cut(s)	2242
MspCI	CTTAAG	2 cut(s)	1141, 2086
MspI	CCGG	6 cut(s)	396, 602, 709, 857, 1534, 1853
MspR9I	CCNGG	2 cut(s)	967, 1615
MunI	CAATTG	1 cut(s)	1168
Mva1269I	GAATGC	2 cut(s)	590, 592
MvaI	CCWGG	2 cut(s)	967, 1615
MvnI	CGCG	2 cut(s)	183, 247
MwoI	GCNNNNNNNGC	9 cut(s)	72, 382, 739, 845, 1454, 1610, 1802, 1831, 2222
NcoI	CCATGG	2 cut(s)	1539, 2211
NheI	GCTAGC	1 cut(s)	1801
NlaIII	CATG	6 cut(s)	1159, 1405, 1465, 1543, 1964, 2215
NlaIV	GGNNCC	7 cut(s)	400, 461, 485, 1566, 1905, 2043, 2178
NmuCI	GTSAC	3 cut(s)	1136, 1373, 1521
NsbI	TGCGCA	1 cut(s)	1010
NsiI	ATGCAT	1 cut(s)	592
PagI	TCATGA	1 cut(s)	1401
PciSI	GCTCTTC	3 cut(s)	31, 73, 106
PctI	GAATGC	2 cut(s)	590, 592
PdmI	GAANNNNTTC	1 cut(s)	590
PfeI	GAWTC	4 cut(s)	148, 473, 1061, 1186
PflMI	CCANNNNNTGG	1 cut(s)	972
PinAI	ACCGGT	1 cut(s)	395
PkrI	GCNGC	4 cut(s)	77, 1707, 1824, 1959
PleI	GAGTC	1 cut(s)	1747
PpsI	GAGTC	1 cut(s)	1747
PsiI	TTATAA	1 cut(s)	771
Psp124BI	GAGCTC	2 cut(s)	744, 1329
Psp6I	CCWGG	2 cut(s)	965, 1613
PspFI	CCCAGC	2 cut(s)	810, 956
PspGI	CCWGG	2 cut(s)	965, 1613
PspN4I	GGNNCC	7 cut(s)	400, 461, 485, 1566, 1905, 2043, 2178
PspPI	GGNCC	3 cut(s)	459, 1536, 1574
PsrI	GAACNNNNNNTAC	2 cut(s)	2238, 2270
PstI	CTGCAG	1 cut(s)	1959
PstNI	CAGNNNCTG	3 cut(s)	119, 972, 1448
PsuI	RGATCY	4 cut(s)	483, 969, 1564, 1789
PvuII	CAGCTG	1 cut(s)	2242
RsaI	GTAC	6 cut(s)	530, 1025, 1408, 1988, 2043, 2163
RsaNI	GTAC	6 cut(s)	529, 1024, 1407, 1987, 2042, 2162
RseI	CAYNNNNRTG	1 cut(s)	413
SacI	GAGCTC	2 cut(s)	744, 1329
SapI	GCTCTTC	3 cut(s)	31, 73, 106
SaqAI	TTAA	8 cut(s)	786, 1142, 1413, 1580, 1655, 1767, 2087, 2103
SatI	GCNGC	4 cut(s)	76, 1706, 1823, 1958
Sau96I	GGNCC	3 cut(s)	459, 1536, 1574
SchI	GAGTC	1 cut(s)	1748
ScrFI	CCNGG	2 cut(s)	967, 1615
SduI	GDGCHC	4 cut(s)	403, 744, 1329, 2078
SfaNI	GCATC	3 cut(s)	664, 1030, 1466
SfcI	CTRYAG	3 cut(s)	1449, 1955, 2182
SinI	GGWCC	2 cut(s)	459, 1536
SmiMI	CAYNNNNRTG	1 cut(s)	413
SmlI	CTYRAG	2 cut(s)	1141, 2086
SmoI	CTYRAG	2 cut(s)	1141, 2086
Sse9I	AATT	7 cut(s)	554, 1068, 1098, 1168, 1340, 1664, 2193
SsiI	CCGC	9 cut(s)	46, 64, 73, 76, 136, 160, 181, 245, 1076
SspI	AATATT	2 cut(s)	947, 1272
SspMI	CTAG	4 cut(s)	222, 1028, 1193, 1802
SstI	GAGCTC	2 cut(s)	744, 1329
StyD4I	CCNGG	2 cut(s)	965, 1613
StyI	CCWWGG	2 cut(s)	1539, 2211
TaaI	ACNGT	5 cut(s)	143, 506, 881, 898, 941
TaiI	ACGT	3 cut(s)	219, 581, 2224
TaqI	TCGA	5 cut(s)	327, 660, 1038, 1634, 2200
TasI	AATT	7 cut(s)	554, 1068, 1098, 1168, 1340, 1664, 2193
TatI	WGTACW	2 cut(s)	1406, 1986
TauI	GCSGC	1 cut(s)	78
TfiI	GAWTC	4 cut(s)	148, 473, 1061, 1186
Tru1I	TTAA	8 cut(s)	786, 1142, 1413, 1580, 1655, 1767, 2087, 2103
Tru9I	TTAA	8 cut(s)	786, 1142, 1413, 1580, 1655, 1767, 2087, 2103
TscAI	CASTG	5 cut(s)	484, 511, 886, 1611, 1996
TseFI	GTSAC	3 cut(s)	1136, 1373, 1521
TseI	GCWGC	3 cut(s)	1705, 1822, 1957
Tsp45I	GTSAC	3 cut(s)	1136, 1373, 1521
TspDTI	ATGAA	7 cut(s)	318, 337, 809, 1038, 1074, 1478, 1749
TspGWI	ACGGA	1 cut(s)	1013
TspRI	CASTG	5 cut(s)	484, 511, 886, 1611, 1996
Van91I	CCANNNNNTGG	1 cut(s)	972
Vha464I	CTTAAG	2 cut(s)	1141, 2086
VpaK11BI	GGWCC	2 cut(s)	459, 1536
XapI	RAATTY	2 cut(s)	1068, 1664
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1346
XmiI	GTMKAC	1 cut(s)	2247
XmnI	GAANNNNTTC	1 cut(s)	590
XspI	CTAG	4 cut(s)	222, 1028, 1193, 1802
Zsp2I	ATGCAT	1 cut(s)	592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.