Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Reverse (-)

54939915 .. 54949771

Gene Structure 9857 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00205380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3513 bp

ATGGAGAGAGCAGCTCTCCTTCGCTCTTCTCTTTCCTGCACTAATAGAGCCTTCTTCTCCTTCCGCCCCCCTCTATCCCATTCTTTCGATTCTCTCGCTCCTCCTCTTCTTCTTCTTCTGCTCTCAGAACCCATCGCCACCGTCCGATTCTCCGACCCTCCTCCGGCCGCCCCCTCCGCCGCACCAGCCTCTTACCACTCCCCGCCGCTTCCACCCACTTCCCCACGCGCTTCTCCTCCCTCTCCCTACGCGCTTGTAGAGTTCTCTGGGGTTTCCGATGAGGTTGCGGAACAGCTGGGATTCGAGAAGGTCGCTGAAGAGTTTATAGGAGAGTGTAAATCCAAAGCTCTGCTCTTTAAGCACAAAAAGACCGGTGCACAGGTGATCTCTGTTTCCAACGACGATGAGAACAAGGTTTTCGGCATTGTTTTCCGAACACCACCAAATGATTCGACTGGAATCCCACACATTTTGGAACACAGTGTGTTGTGTGGTTCTAGGAAATACCCTTTGAAAGAGCCGTTTGTGGAACTGTTGAAAGGGAGCTTGAATACTTTTCTCAATGCATTCACCTATCCTGATAGGACTTGCTACCCTGTTGCCTCCACAAATTCAAAGGATTTCTATAATCTGGTCGATGTGTACCTGGATGCTGTGTTCTTTCCAAAATGCGTGGAGGATTTCCAGACTTTCCAGCAGGAGGGCTGGCACTATGAGCTCAACGATCCTTCTGAAGACATATCTTATAAAGGAGTTGCTCTTTTTCCAGACAATACCTATGGTGTGGACAGTGGAGGTGATCCAAAAGTTATTCCCAAACTGACATATGAGGAATTTAAGTTAGACTTTCTTATCTGTGGAAAGATGGTGATGTTATCTTCTTTCACCCCAAGTACAGAATTTCACCGTAAATATTACCATCCGAGCAATGCCAGGATTTGGTTTTATGGAGATGATGATCCAACTGAGCGCCTACGCATTTTAAGTGAGTACCTAGATATGTTTGATGCAAGTTCAGCTCCAAATGAATCGAGGGTTCAAACACAAAAACTATTTTCGGAACCAGTTAGGATTTCTGAGACATATCCTGCTGGTGAAGGTGGTGACTTAAAGAAAAAAGACATGGTATGCATCAATTGGCTGCTCTCTGAGAAACCCTTAGACTTGGAAACTGAGCTTGCCCTTGGGTTTTTGGATCATCTTATGTTGGGAACTCCTGCCTCTCCATTGAGGAAAATATTACTGGAAAGTGGCTTGGGAGAAGCTATCATTGGAGGTGGGGTTGAAGATGAGCTACTTCAACCTCAATTTAGTATTGGGTTGAAGGGTGTTTCTCAAGATGATATTCAAAAGGTAGAAGAATTAGTCATGAGTACACTTAAAAAATTGGCGGACGAAGGGTTCGATACAGCTGCTGTAGAAGCATCCATGAATACCATTGAGTTTTCTCTCAGGGAAAACAACACCGGATCATTTCCTCGTGGGTTGTCCTTAATGCTTCGATCCATGGGTAAGTGGATATATGATATGGATCCATTTCAGCCATTGAAGTACGAGAAACCTTTACTGGCACTAAAAGCAAGAATAGAACAGGAAGGCTCTAAAGCCGTTTTCTCTCCTTTAATAGAGAAATTCATTTTGAACAATCCTCATCGAGTTGTGGTAGAAATGCAGCCTGACCCAGAAAAAGCTTCTCGTGATGAAGCAGCTGAGAAAGAAATTTTGGAGAAAGTAAAAGCAGGTATGACAGAAGAAGATCTTGCTGAGCTAGCACGCGCTACACAAGAGCTGAAACTGAAGCAGGAAACTCCTGATCCACCAGAAGCTCTGAGAAGTGTTCCAAGCCTCTCTCTACGTGACATTCCAAAAGAACCTATTCTTGTTCCCACAGAGGTTGGGGAGATCAGTGGAGTTAAAGTTTTGCAGCATGACCTGTTCACAAATGATGTTCTCTACACTGAAGTTGTGTTCAATATGAGTTCACTAAAGCAAGAGCTCCTTCCTTTGGTACCTCTCTTTTGTCAATCATTGCTGGAGATGGGAACCAAAGATTTGAGTTTTGTACAACTTAATCAGTTGATTGGAAGAAAAACTGGAGGGATATCAGTTTATCCACTGACATCATCTGTCCGGGGCAAGGAAGATGCCTGTAGCCATATAATTGTTCGAGGCAAAGCCATGGCAGGACGTGCTGATGATCTATTTCATCTGATCAACTGTATTCTTCAAGAAGTCCAATTTACAGACCAACAGCGGTTTAAGCAGTTTGTTTCCCAAAGCAAAGCTAGAATGGAGAACCGATTGAGAGGTAGCGGTCATGGAATTGCTGCTGCAAGGATGGATGCAAAGCTAAATGTCGCGGGTTGGATTTCTGAACAAATGGGTGGTTTCAGTTACTTGGAGTTTCTACAAGCTCTTGAAGAGAAAGTTGATGATGATTGGGAGAGAATATCTTCATCTCTTGAGGAGATTCGCAAGTCCTTACTTTCACGGGAAGGTTGCTTGATTAATATGACTGCTGAAGGAAAGAATCTTACCAACTCTCAGAAGTTCGTTGGCAAATTTCTGGATTTGCTGCCTCGCAGTTCTCCCATTGCAACAACTACTTGGAATGCTCAACTACCTTCTACAAATGAAGCCATTGTTATACCTACTCAGGTTAATTATGTTGGAAAAGCAGCCAACATTTATGAGACTGGGTATCAACTTAATGGAACTGCATATGTTATCTCCAAATACATAAGTAATACATGGTTGTGGGATCGTGTACGAGTTAGTGGTGGAGCATATGGAGGCTTTTGCGACTTTGATAGTCACTCCGGTGTGTTCTCCTTCTTATCTTATCGCGACCCCAACTTGTTAAAGACACTTGATATATATGATGGAACTGGGGAATTTCTTAGACAACTAGATATGGATGAAGAAACTCTCACAAAGTCCATTATTGGAACAATTGGAGACGTTGATTCATACCAGCTTCCTGATGCCAAAGGTTATAGTAGTTTGATGCGGCATTTATTGGGAGTTTCAGATGAGGAAAGGAAAATTAGACGAGAAGAAATATTGTCAACCAGCTTGAAGGACTTCAAGGAGTTTGCAAATGCAATTGATGCAGTTAAAGACAAAGGGGTTGCTGTTGCAGTGGCATCTCCAGATGATGTGGATGCAGCACAGAAGGAAAGATCAAACTTATTTGAAAGTTTTGCTCGTCTTGTTTTTGGGTCTGCTCAATTCCTCAACATCTCCAGCAGATCCGGAAGTCAAAGTCACAAGAGAAAATCTCTTCTTTCGGTAGTTGGCAATGACTTGGATGCGGTGGTAGTAGATGCCCACCCTTTTGTTGGGATTTCTGATTGTGATATTGATGGCGAGGTTGTAGTTGAGAGTGTTGTTGGTGTTGTTAAAATTGAATTGGGTCAGAGAGGCATTTGTGACTTTGAATTTAGGCTCTTGGGGAAGGAAGATGAGCCAGAATATGAGGAAGGACAAGGAGAAGCAGATGATGAAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

1170

Amino Acids

130.03

Weight (kDa)

4.89

Isoelectric Point (pI)

42.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	148 - 223	4.3e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	300 - 478	4.8e-23	Peptidase M16 inactive domain
M16C_assoc	PF08367	557 - 806	3.5e-77	Peptidase M16C associated
PreP_C	PF22516	878 - 1050	2.6e-79	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	747
Acc36I	ACCTGC	1 cut(s)	1730
Acc65I	GGTACC	1 cut(s)	2008
AccB1I	GGYRCC	1 cut(s)	2008
AccB7I	CCANNNNNTGG	1 cut(s)	939
AccII	CGCG	5 cut(s)	228, 251, 1776, 2360, 2847
AccIII	TCCGGA	1 cut(s)	3254
AcoI	YGGCCR	1 cut(s)	165
AcsI	RAATTY	8 cut(s)	610, 833, 899, 1631, 1719, 2561, 2894, 3440
AcuI	CTGAAG	5 cut(s)	336, 753, 1817, 1980, 2541
AdeI	CACNNNGTG	1 cut(s)	484
AfaI	GTAC	8 cut(s)	644, 895, 992, 1375, 1553, 2010, 2064, 2769
AfiI	CCNNNNNNNGG	6 cut(s)	163, 700, 939, 2005, 2137, 3341
AgeI	ACCGGT	1 cut(s)	371
AjiI	CACGTC	1 cut(s)	2189
AjnI	CCWGG	2 cut(s)	645, 932
AjuI	GAANNNNNNNTTGG	4 cut(s)	1254, 1286, 1706, 1738
AleI	CACNNNNGTG	1 cut(s)	2820
AloI	GAACNNNNNNTCC	4 cut(s)	641, 673, 1385, 1417
Alw21I	GWGCWC	3 cut(s)	379, 720, 1998
Alw26I	GTCTC	3 cut(s)	1073, 2687, 2952
Alw44I	GTGCAC	1 cut(s)	375
AlwNI	CAGNNNCTG	1 cut(s)	1415
Aor13HI	TCCGGA	1 cut(s)	3254
AoxI	GGCC	1 cut(s)	165
ApaLI	GTGCAC	1 cut(s)	375
ApoI	RAATTY	8 cut(s)	610, 833, 899, 1631, 1719, 2561, 2894, 3440
ArsI	GACNNNNNNTTYG	2 cut(s)	1385, 1417
AseI	ATTAAT	1 cut(s)	2508
AsiGI	ACCGGT	1 cut(s)	371
Asp700I	GAANNNNTTC	3 cut(s)	1875, 2452, 3083
Asp718I	GGTACC	1 cut(s)	2008
AspLEI	GCGC	4 cut(s)	230, 253, 972, 1778
AsuC2I	CCSGG	1 cut(s)	2132
AsuHPI	GGTGA	8 cut(s)	394, 562, 809, 877, 880, 896, 1106, 1115
AsuNHI	GCTAGC	1 cut(s)	1768
BaeGI	GKGCMC	1 cut(s)	379
BamHI	GGATCC	1 cut(s)	1531
BanI	GGYRCC	1 cut(s)	2008
BanII	GRGCYC	2 cut(s)	720, 1998
BarI	GAAGNNNNNNTAC	2 cut(s)	1278, 1310
BauI	CACGAG	2 cut(s)	1479, 1695
BbsI	GAAGAC	1 cut(s)	741
Bbv12I	GWGCWC	3 cut(s)	379, 720, 1998
BccI	CCATC	7 cut(s)	140, 859, 927, 2032, 2332, 2876, 3359
BceAI	ACGGC	2 cut(s)	505, 1592
BcgI	CGANNNNNNTGC	4 cut(s)	266, 300, 1394, 1428
BciT130I	CCWGG	2 cut(s)	647, 934
BclI	TGATCA	1 cut(s)	2211
BcnI	CCSGG	1 cut(s)	2132
BcoDI	GTCTC	3 cut(s)	1073, 2687, 2952
BfaI	CTAG	5 cut(s)	498, 995, 1769, 2286, 2909
BfmI	CTRYAG	2 cut(s)	1416, 2149
BfoI	RGCGCY	1 cut(s)	973
BfuAI	ACCTGC	1 cut(s)	1730
BglII	AGATCT	1 cut(s)	1756
BlpI	GCTNAGC	1 cut(s)	1764
Bme1390I	CCNGG	3 cut(s)	647, 934, 2132
BmgBI	CACGTC	1 cut(s)	2189
BmiI	GGNNCC	4 cut(s)	1062, 1533, 2010, 2044
BmrFI	CCNGG	3 cut(s)	647, 934, 2132
BmrI	ACTGGG	2 cut(s)	2706, 2898
BmtI	GCTAGC	1 cut(s)	1772
BmuI	ACTGGG	2 cut(s)	2706, 2898
BpiI	GAAGAC	1 cut(s)	741
BplI	GAGNNNNNCTC	4 cut(s)	30, 32, 3265, 3297
BpmI	CTGGAG	4 cut(s)	2054, 2115, 3135, 3229
Bpu1102I	GCTNAGC	1 cut(s)	1764
BpuEI	CTTGAG	2 cut(s)	1320, 2483
BpuMI	CCSGG	1 cut(s)	2132
BsaAI	YACGTR	1 cut(s)	1856
BsaBI	GATNNNNATC	2 cut(s)	957, 1530
BsaJI	CCNNGG	4 cut(s)	1183, 1506, 2131, 2178
BsaWI	WCCGGW	4 cut(s)	371, 1466, 2819, 3254
BsaXI	ACNNNNNCTCC	4 cut(s)	1266, 1296, 1902, 1932
Bsc4I	CCNNNNNNNGG	6 cut(s)	163, 700, 939, 2005, 2137, 3341
Bse118I	RCCGGY	1 cut(s)	371
Bse1I	ACTGG	7 cut(s)	460, 1064, 1248, 1572, 2098, 2701, 2893
Bse3DI	GCAATG	4 cut(s)	934, 2027, 2592, 3307
Bse8I	GATNNNNATC	2 cut(s)	957, 1530
BseAI	TCCGGA	1 cut(s)	3254
BseBI	CCWGG	2 cut(s)	647, 934
BseDI	CCNNGG	4 cut(s)	1183, 1506, 2131, 2178
BseGI	GGATG	8 cut(s)	655, 919, 1424, 2343, 2347, 2923, 3169, 3316
BseJI	GATNNNNATC	2 cut(s)	957, 1530
BseLI	CCNNNNNNNGG	6 cut(s)	163, 700, 939, 2005, 2137, 3341
BseMI	GCAATG	4 cut(s)	934, 2027, 2592, 3307
BseNI	ACTGG	7 cut(s)	460, 1064, 1248, 1572, 2098, 2701, 2893
BseRI	GAGGAG	5 cut(s)	90, 93, 150, 225, 2480
BseSI	GKGCMC	1 cut(s)	379
BseX3I	CGGCCG	1 cut(s)	165
BseYI	CCCAGC	1 cut(s)	295
BsgI	GTGCAG	1 cut(s)	22
Bsh1236I	CGCG	5 cut(s)	228, 251, 1776, 2360, 2847
Bsh1285I	CGRYCG	1 cut(s)	168
BshFI	GGCC	1 cut(s)	167
BshNI	GGYRCC	1 cut(s)	2008
BshTI	ACCGGT	1 cut(s)	371
BsiEI	CGRYCG	1 cut(s)	168
BsiHKAI	GWGCWC	3 cut(s)	379, 720, 1998
BsiSI	CCGG	6 cut(s)	164, 372, 1467, 2131, 2820, 3255
BslI	CCNNNNNNNGG	6 cut(s)	163, 700, 939, 2005, 2137, 3341
BsmAI	GTCTC	3 cut(s)	1073, 2687, 2952
BsmBI	CGTCTC	1 cut(s)	2952
BsmI	GAATGC	2 cut(s)	566, 2617
BsnI	GGCC	1 cut(s)	167
Bsp1286I	GDGCHC	3 cut(s)	379, 720, 1998
Bsp13I	TCCGGA	1 cut(s)	3254
Bsp1407I	TGTACA	1 cut(s)	2062
Bsp1720I	GCTNAGC	1 cut(s)	1764
Bsp19I	CCATGG	2 cut(s)	1506, 2178
Bsp68I	TCGCGA	1 cut(s)	2847
BspANI	GGCC	1 cut(s)	167
BspEI	TCCGGA	1 cut(s)	3254
BspFNI	CGCG	5 cut(s)	228, 251, 1776, 2360, 2847
BspHI	TCATGA	1 cut(s)	1368
BspLI	GGNNCC	4 cut(s)	1062, 1533, 2010, 2044
BspMI	ACCTGC	1 cut(s)	1730
BspOI	GCTAGC	1 cut(s)	1772
BspQI	GCTCTTC	1 cut(s)	31
BspT107I	GGYRCC	1 cut(s)	2008
BsrDI	GCAATG	4 cut(s)	934, 2027, 2592, 3307
BsrFI	RCCGGY	1 cut(s)	371
BsrGI	TGTACA	1 cut(s)	2062
BsrI	ACTGG	7 cut(s)	460, 1064, 1248, 1572, 2098, 2701, 2893
BssAI	RCCGGY	1 cut(s)	371
BssECI	CCNNGG	4 cut(s)	1183, 1506, 2131, 2178
BssSI	CACGAG	2 cut(s)	1479, 1695
BssT1I	CCWWGG	3 cut(s)	1183, 1506, 2178
Bst2BI	CACGAG	2 cut(s)	1479, 1695
Bst2UI	CCWGG	2 cut(s)	647, 934
Bst4CI	ACNGT	6 cut(s)	142, 482, 534, 791, 908, 2219
Bst6I	CTCTTC	5 cut(s)	31, 111, 312, 2415, 3288
BstAPI	GCANNNNNTGC	2 cut(s)	2189, 3110
BstAUI	TGTACA	1 cut(s)	2062
BstBAI	YACGTR	1 cut(s)	1856
BstC8I	GCNNGC	4 cut(s)	707, 1179, 1770, 1774
BstDSI	CCRYGG	2 cut(s)	1506, 2178
BstF5I	GGATG	8 cut(s)	655, 919, 1424, 2343, 2347, 2923, 3169, 3316
BstFNI	CGCG	5 cut(s)	228, 251, 1776, 2360, 2847
BstH2I	RGCGCY	1 cut(s)	973
BstHHI	GCGC	4 cut(s)	230, 253, 972, 1778
BstMAI	GTCTC	3 cut(s)	1073, 2687, 2952
BstMCI	CGRYCG	1 cut(s)	168
BstNI	CCWGG	2 cut(s)	647, 934
BstSCI	CCNGG	3 cut(s)	645, 932, 2130
BstSFI	CTRYAG	2 cut(s)	1416, 2149
BstSLI	GKGCMC	1 cut(s)	379
BstUI	CGCG	5 cut(s)	228, 251, 1776, 2360, 2847
BstV2I	GAAGAC	1 cut(s)	741
BstX2I	RGATCY	3 cut(s)	1531, 1756, 3251
BstYI	RGATCY	3 cut(s)	1531, 1756, 3251
BstZI	CGGCCG	1 cut(s)	165
BsuRI	GGCC	1 cut(s)	167
BtgI	CCRYGG	2 cut(s)	1506, 2178
BtgZI	GCGATG	1 cut(s)	118
BtrI	CACGTC	1 cut(s)	2189
BtsCI	GGATG	8 cut(s)	655, 919, 1424, 2343, 2347, 2923, 3169, 3316
BtsI	GCAGTG	1 cut(s)	3147
BtsIMutI	CAGTG	6 cut(s)	487, 796, 1912, 1956, 2114, 3147
BtuMI	TCGCGA	1 cut(s)	2847
BveI	ACCTGC	1 cut(s)	1730
Cac8I	GCNNGC	4 cut(s)	707, 1179, 1770, 1774
CaiI	CAGNNNCTG	1 cut(s)	1415
CciI	TCATGA	1 cut(s)	1368
CfoI	GCGC	4 cut(s)	230, 253, 972, 1778
Cfr10I	RCCGGY	1 cut(s)	371
Csp6I	GTAC	8 cut(s)	643, 894, 991, 1374, 1552, 2009, 2063, 2768
CspAI	ACCGGT	1 cut(s)	371
CspCI	CAANNNNNGTGG	6 cut(s)	453, 488, 654, 689, 1876, 1911
CviAII	CATG	8 cut(s)	1123, 1369, 1429, 1507, 1928, 2179, 2318, 2751
CviQI	GTAC	8 cut(s)	643, 894, 991, 1374, 1552, 2009, 2063, 2768
DraIII	CACNNNGTG	1 cut(s)	484
EaeI	YGGCCR	1 cut(s)	165
EagI	CGGCCG	1 cut(s)	165
Eam1104I	CTCTTC	5 cut(s)	31, 111, 312, 2415, 3288
EarI	CTCTTC	5 cut(s)	31, 111, 312, 2415, 3288
EciI	GGCGGA	3 cut(s)	53, 166, 1406
Ecl136II	GAGCTC	2 cut(s)	718, 1996
EclXI	CGGCCG	1 cut(s)	165
Eco130I	CCWWGG	3 cut(s)	1183, 1506, 2178
Eco24I	GRGCYC	2 cut(s)	720, 1998
Eco32I	GATATC	1 cut(s)	2103
Eco52I	CGGCCG	1 cut(s)	165
Eco53kI	GAGCTC	2 cut(s)	718, 1996
Eco57I	CTGAAG	5 cut(s)	336, 753, 1817, 1980, 2541
EcoICRI	GAGCTC	2 cut(s)	718, 1996
EcoRII	CCWGG	2 cut(s)	645, 932
EcoRV	GATATC	1 cut(s)	2103
EcoT14I	CCWWGG	3 cut(s)	1183, 1506, 2178
EcoT22I	ATGCAT	2 cut(s)	568, 1133
EcoT38I	GRGCYC	2 cut(s)	720, 1998
ErhI	CCWWGG	3 cut(s)	1183, 1506, 2178
Esp3I	CGTCTC	1 cut(s)	2952
FaeI	CATG	8 cut(s)	1126, 1372, 1432, 1510, 1931, 2182, 2321, 2754
FalI	AAGNNNNNCTT	6 cut(s)	830, 862, 1743, 1775, 1983, 2015
FatI	CATG	8 cut(s)	1122, 1368, 1428, 1506, 1927, 2178, 2317, 2750
FauI	CCCGC	2 cut(s)	210, 2353
FauNDI	CATATG	3 cut(s)	826, 2722, 2788
FbaI	TGATCA	1 cut(s)	2211
FokI	GGATG	8 cut(s)	662, 906, 1411, 2350, 2354, 2930, 3176, 3323
FriOI	GRGCYC	2 cut(s)	720, 1998
FspBI	CTAG	5 cut(s)	498, 995, 1769, 2286, 2909
GlaI	GCGC	4 cut(s)	229, 252, 971, 1777
GsaI	CCCAGC	1 cut(s)	299
GsuI	CTGGAG	4 cut(s)	2054, 2115, 3135, 3229
HaeII	RGCGCY	1 cut(s)	973
HaeIII	GGCC	1 cut(s)	167
HapII	CCGG	6 cut(s)	164, 372, 1467, 2131, 2820, 3255
HhaI	GCGC	4 cut(s)	230, 253, 972, 1778
Hin1II	CATG	8 cut(s)	1126, 1372, 1432, 1510, 1931, 2182, 2321, 2754
Hin6I	GCGC	4 cut(s)	228, 251, 970, 1776
HinP1I	GCGC	4 cut(s)	228, 251, 970, 1776
HincII	GTYRAC	1 cut(s)	3069
HindII	GTYRAC	1 cut(s)	3069
HindIII	AAGCTT	1 cut(s)	1689
HinfI	GANTC	9 cut(s)	89, 147, 300, 449, 459, 1028, 2470, 2530, 2966
HpaII	CCGG	6 cut(s)	164, 372, 1467, 2131, 2820, 3255
HphI	GGTGA	8 cut(s)	394, 562, 809, 877, 880, 896, 1106, 1115
Hpy166II	GTNNAC	8 cut(s)	377, 643, 787, 1376, 1938, 1982, 2768, 3069
Hpy8I	GTNNAC	8 cut(s)	377, 643, 787, 1376, 1938, 1982, 2768, 3069
Hpy99I	CGWCG	1 cut(s)	404
HpyCH4III	ACNGT	6 cut(s)	142, 482, 534, 791, 908, 2219
HpyCH4IV	ACGT	3 cut(s)	1855, 2188, 2961
HpySE526I	ACGT	3 cut(s)	1855, 2188, 2961
Hsp92II	CATG	8 cut(s)	1126, 1372, 1432, 1510, 1931, 2182, 2321, 2754
HspAI	GCGC	4 cut(s)	228, 251, 970, 1776
Kpn2I	TCCGGA	1 cut(s)	3254
KpnI	GGTACC	1 cut(s)	2012
Ksp22I	TGATCA	1 cut(s)	2211
LguI	GCTCTTC	1 cut(s)	31
LmnI	GCTCC	5 cut(s)	103, 543, 1024, 2001, 2783
MaeI	CTAG	5 cut(s)	498, 995, 1769, 2286, 2909
MaeII	ACGT	3 cut(s)	1855, 2188, 2961
MaeIII	GTNAC	6 cut(s)	1103, 1856, 2393, 2813, 3266, 3431
MfeI	CAATTG	3 cut(s)	1135, 2952, 3105
MflI	RGATCY	3 cut(s)	1531, 1756, 3251
MhlI	GDGCHC	3 cut(s)	379, 720, 1998
MmeI	TCCRAC	5 cut(s)	177, 420, 986, 2345, 2650
Mph1103I	ATGCAT	2 cut(s)	568, 1133
MroI	TCCGGA	1 cut(s)	3254
MroXI	GAANNNNTTC	3 cut(s)	1875, 2452, 3083
MslI	CAYNNNNRTG	2 cut(s)	2755, 2820
MspA1I	CMGCKG	4 cut(s)	295, 1412, 1709, 2254
MspI	CCGG	6 cut(s)	164, 372, 1467, 2131, 2820, 3255
MspR9I	CCNGG	3 cut(s)	647, 934, 2132
MunI	CAATTG	3 cut(s)	1135, 2952, 3105
Mva1269I	GAATGC	2 cut(s)	566, 2617
MvaI	CCWGG	2 cut(s)	647, 934
MvnI	CGCG	5 cut(s)	228, 251, 1776, 2360, 2847
NciI	CCSGG	1 cut(s)	2132
NcoI	CCATGG	2 cut(s)	1506, 2178
NdeI	CATATG	3 cut(s)	826, 2722, 2788
NheI	GCTAGC	1 cut(s)	1768
NlaIII	CATG	8 cut(s)	1126, 1372, 1432, 1510, 1931, 2182, 2321, 2754
NlaIV	GGNNCC	4 cut(s)	1062, 1533, 2010, 2044
NmuCI	GTSAC	5 cut(s)	1103, 1856, 2813, 3266, 3431
NruI	TCGCGA	1 cut(s)	2847
NsiI	ATGCAT	2 cut(s)	568, 1133
OliI	CACNNNNGTG	1 cut(s)	2820
PagI	TCATGA	1 cut(s)	1368
PciSI	GCTCTTC	1 cut(s)	31
PcsI	WCGNNNNNNNCGW	1 cut(s)	1401
PctI	GAATGC	2 cut(s)	566, 2617
PdmI	GAANNNNTTC	3 cut(s)	1875, 2452, 3083
PfeI	GAWTC	9 cut(s)	89, 147, 300, 449, 459, 1028, 2470, 2530, 2966
PflMI	CCANNNNNTGG	1 cut(s)	939
PinAI	ACCGGT	1 cut(s)	371
Ppu21I	YACGTR	1 cut(s)	1856
PshBI	ATTAAT	1 cut(s)	2508
PsiI	TTATAA	1 cut(s)	747
Psp124BI	GAGCTC	2 cut(s)	720, 1998
Psp6I	CCWGG	2 cut(s)	645, 932
PspFI	CCCAGC	1 cut(s)	295
PspGI	CCWGG	2 cut(s)	645, 932
PspN4I	GGNNCC	4 cut(s)	1062, 1533, 2010, 2044
PstNI	CAGNNNCTG	1 cut(s)	1415
PsuI	RGATCY	3 cut(s)	1531, 1756, 3251
PvuII	CAGCTG	3 cut(s)	295, 1412, 1709
RruI	TCGCGA	1 cut(s)	2847
RsaI	GTAC	8 cut(s)	644, 895, 992, 1375, 1553, 2010, 2064, 2769
RsaNI	GTAC	8 cut(s)	643, 894, 991, 1374, 1552, 2009, 2063, 2768
RseI	CAYNNNNRTG	2 cut(s)	2755, 2820
SacI	GAGCTC	2 cut(s)	720, 1998
SapI	GCTCTTC	1 cut(s)	31
ScrFI	CCNGG	3 cut(s)	647, 934, 2132
SduI	GDGCHC	3 cut(s)	379, 720, 1998
SfcI	CTRYAG	2 cut(s)	1416, 2149
SmiMI	CAYNNNNRTG	2 cut(s)	2755, 2820
SmlI	CTYRAG	2 cut(s)	1335, 2462
SmoI	CTYRAG	2 cut(s)	1335, 2462
SspI	AATATT	3 cut(s)	914, 1239, 3063
SspMI	CTAG	5 cut(s)	498, 995, 1769, 2286, 2909
SstI	GAGCTC	2 cut(s)	720, 1998
StyD4I	CCNGG	3 cut(s)	645, 932, 2130
StyI	CCWWGG	3 cut(s)	1183, 1506, 2178
TaaI	ACNGT	6 cut(s)	142, 482, 534, 791, 908, 2219
TaiI	ACGT	3 cut(s)	1858, 2191, 2964
TaqI	TCGA	9 cut(s)	87, 303, 452, 636, 1031, 1404, 1501, 1654, 2167
TatI	WGTACW	3 cut(s)	893, 1373, 2062
TauI	GCSGC	4 cut(s)	170, 182, 208, 3013
TfiI	GAWTC	9 cut(s)	89, 147, 300, 449, 459, 1028, 2470, 2530, 2966
TscAI	CASTG	6 cut(s)	487, 796, 1912, 1963, 2121, 3147
TseFI	GTSAC	5 cut(s)	1103, 1856, 2813, 3266, 3431
Tsp45I	GTSAC	5 cut(s)	1103, 1856, 2813, 3266, 3431
TspDTI	ATGAA	9 cut(s)	1041, 1445, 1624, 1716, 2195, 2445, 2649, 2934, 2958
TspRI	CASTG	6 cut(s)	487, 796, 1912, 1963, 2121, 3147
Van91I	CCANNNNNTGG	1 cut(s)	939
VneI	GTGCAC	1 cut(s)	375
VspI	ATTAAT	1 cut(s)	2508
XapI	RAATTY	8 cut(s)	610, 833, 899, 1631, 1719, 2561, 2894, 3440
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3338
XmnI	GAANNNNTTC	3 cut(s)	1875, 2452, 3083
XspI	CTAG	5 cut(s)	498, 995, 1769, 2286, 2909
Zsp2I	ATGCAT	2 cut(s)	568, 1133

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.