Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Reverse (-)

14070450 .. 14094260

Gene Structure 23811 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G008290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3330 bp

ATGTCATCTTCCATAAGAGCATCATCATCAATAAAATTATCATCAATGAAATCATTATCATCGACTACTGTTCTTTCAATACCAGCCCTGCTCAAAGTAATAGTTTCAGGTCCTACATCTTCTCTACATAACGAAGCAGCTCTCCTTCGCTCTTCTCTTTCCTGCACTAATAGAGCCTTCTTCTCCTTCCGCCTCCCCTCCATCCCATTCTTTCGATTCTCTCGCTCCTCCTCCTCTTCTTCTTCTTCTGCTCTCAGAACCCATCGCCGCCGTCCGATTCTCCGACCCTCCTCCGGCCGCCCCCTCCGCCGCACCAGCCTCTTACCACTCCCCGCCGCTTCCACCCACTTCGCCACGCGCTTCTCCTCCCTCTCCCTACGCGCTGTCGCCACGCCCTTAACCCCCTCGCCGTCAGAGTTCTCTGGGGTTTCCGATGAGGTTGCGGAACAGCTGGGATTCGAGAAGGTCGCCGAAGAGTTTATAGGAGAGTGTAAATCCAAAGCTCTTCTCTTTAAGCACAAAAAGACCGGTGCTCAGGTGATCTCTGTTTCCAACGACGATGAGAACAAGGTTTTCGGCATTGTTTTCCGAACACCACCAAATGATTCGACTGGAATCCCACACATTTTGGAACACAGTGTGTTGTGTGGTTCTAGGAAATACCCTTTGAAAGAGCCGTTTGTGGAACTGTTGAAAGGGAGCTTGAATACTTTTCTCAATGCATTCACCTATCCTGATAGGACTTGCTACCCTGTTGCCTCCACAAATTCAAAGGATTTCTATAATCTGGTCGATGTGTACCTGGATGCTGTGTTCTTTCCAAAATGCGTGGAGGATTTCCAGACTTTCCAGCAGGAGGGCTGGCACTATGAGCTCAACGATCCTTCTGAAGACATATCTTATAAAGGAGTTGTTTTCAATGAGATGAAAGGGGTGTACTCACAGCCTGATAATATATTAGGGCGGATTGCTCAACAGGCTCTTTTTCCAGACAACACCTATGGTGTGGACAGTGGAGGTGATCCAAAAGTTATTCCCAAGCTGACTTATGAGGAATTTAAGGAATTTCACCGTAAATATTACCATCCGAGCAATGCCAGGATTTGGTTCTATGGAGATGATGATCCAACTGAGCGTCTACGCATTTTAAGTGAGTACCTAGACATGTTTGATGCAAGTTCAGCTCCAAATGAATCAAGGGTTCAAACACAAAAACTATTTTCGGAACCAGTTAGGATTTCTGAGACATATCCTTCTGGTGAAGGCGGCGACTTAAAGAAAAAAGGCATGCTTGCCCTTGGGTTTTTAGATCATCTTATGTTGGGAACTCCTGCATCTCCATTGAGGAAAATATTGCTGGAAAGTGGCTTGGGAGAAGCTATCATTGGAGGCGGGGTTGAAGATGAGCTTCTTCAACCTCAATTTAGTATCGGGTTGAAGGGTGTTTCTCAAGATAATATTCAAAAGGTAGAAGAATTAGTCATGAGTACACTTAAAAAATTAGCGGAAGAAGGGTTCGATACAGCTGCTGTAGAAGCATCCATGAATACCATTGAGTTTTCTCTCAGGGAAAACAACACCGGATCATTTCCTCGTGGTTTGTCCTTAATGCTTCGATCCATGGGAAAGTGGATATATGATATGGATCCATTTCAGCCATTGAAGTATGAGAAACCTTTACTGGCACTAAAAGCAAGAATAGAACAGGAAGGCTCTAAAGCCGTTTTCTCTCCTTTAATAGAGAAATTTATTTTGAACAATCTTCATCGAGTTGTGGTAGAAATGCAGCCTGATCCAGAAAAAGCTTCTCGTGATGAAGCAGCTGAGAAAGAAATTTTGGAGAAAGTAAAAGCAGGTATGACGGAAGAAGATCTTGCTGAGCTAGCACGCGCTACACAAGAATTGAAACTGAAGCAGGAAACTCCTGATCCACCAGAAGCTCTGAGAAGTGTTCCAAGCCTCTCTCTGCTTGACATTCCAAAGGAACCTATTCCTGTTCCCACAGAGGTTGGGGATATCAATGGAGTTAAAGTTTTGCAGCATGACCTTTTCACAAATGATGTTCTCTACACTGAAGTTGTGTTCAATATGAGTTCACTCAAGCAAGAGCTCCTTCCTTTGGTACCTCTTTTTTGTCAATCATTGCTGGAGATGGGAACCAAAGATTTGAGTTTTGTGGAACTTAATCAGTTGATTGGAAGAAAAACTGGAGGGATATCAGTTTATCCACTGACATCATCTGTCCGGGGCAAGAAGGATCCCTGTAGCCATATAATTGTTCGAGGCAAAGCCATGGCAGGACGTGCGGCTGATCTATTTCATCTGATCAACTGTATTCTTCAAGAAGTCCAATTTACAGACCAGCTGCGGTTTAAGCAGTTTGTTTCCCAAAGCAAAGCTAGAATGGAGAACCGATTGAGAGGTAGCGGTCATGGAATTGCTGCTGCAAGGATGGATGCAAAGCTAAATGTTGCGGGGTGGATTTCTGAGCAAATGGGTGGTTTCAGTTACTTGGAGTTTCTACAAGCTCTTGAAGAAAAAGTTGATAATGATTGGGAGAGAATATCTTCATCTCTTGAGGAGATTCGCAAGTCCTTACTTTCACAGGAGGGTTGCTTGATTAATATGACTGCTGAAGGAAAGAATCTTACCAACTCTGAGAAGTTTGTTGGCGAATTTCTTAATTTGCTGCCTCACAAATCTCCCCTTGCAACAACTACTTGGAATGCTCGATTACCTTCTATAAATGAAGCCATTGTTATACCTACTCAGGTTAATTATGTTGGAAAAGCAGCCAACATTTATGACACTGGGTATCAACTTAATGGAAGTGCATATGTTATCTCTAAATACATAAGTAATACATGGTTGTGGGATCGTGTACGAGTTAGTGGTGGAGCATATGGAGGTTTTTGCGACTTCGACAGTCACTCCGGTGTGTTCTCCTTCTTATCTTATCGTGACCCCAACTTGTTAAAGACACTTGATATATATGATGGAACTGGGGAATTTCTACGACAACTAGATTTGGATGAAGAAACTCTCACAAAGTCCATCATTGGAACAATTGGAGATGTTGATTCATACCAGCTTCCTGATGCCAAAGGTTATAGTAGTTTGATGCGGTATTTATTGGGAGTTTCAGATGAGGAAAGGAAAATTAGGCGAGAAGAGATATTGTCAACAAGCTTGAAGGACTTCAAGGAGTTTGCAAATGCAATTGATGCAGTTAAAGACAAAGGGGTTTCTGTTGCAGTGGCATCTCCAGATGATGTTGATGCAGCACAAAAGGAAAGATCAAACTTATTTGAAGTAAAGAAAGCCCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

1109

Amino Acids

123.38

Weight (kDa)

5.69

Isoelectric Point (pI)

44.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	200 - 275	3.7e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	347 - 516	6.2e-23	Peptidase M16 inactive domain
M16C_assoc	PF08367	595 - 844	1.2e-75	Peptidase M16C associated
PreP_C	PF22516	916 - 1088	1.1e-80	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	903
Acc36I	ACCTGC	1 cut(s)	1844
Acc65I	GGTACC	1 cut(s)	2122
AccB1I	GGYRCC	1 cut(s)	2122
AccB7I	CCANNNNNTGG	1 cut(s)	1104
AccI	GTMKAC	1 cut(s)	1138
AccII	CGCG	3 cut(s)	358, 381, 1890
AcoI	YGGCCR	1 cut(s)	295
AcsI	RAATTY	7 cut(s)	766, 1055, 1064, 1745, 1833, 2675, 3008
AcuI	CTGAAG	4 cut(s)	909, 1931, 2094, 2655
AdeI	CACNNNGTG	1 cut(s)	640
AfaI	GTAC	6 cut(s)	800, 938, 1157, 1489, 2124, 2883
AfiI	CCNNNNNNNGG	4 cut(s)	293, 856, 1104, 2119
AflIII	ACRYGT	1 cut(s)	1164
AgeI	ACCGGT	1 cut(s)	527
AjiI	CACGTC	1 cut(s)	2303
AjnI	CCWGG	2 cut(s)	801, 1097
AjuI	GAANNNNNNNTTGG	4 cut(s)	1368, 1400, 1820, 1852
AleI	CACNNNNGTG	1 cut(s)	2934
AloI	GAACNNNNNNTCC	4 cut(s)	797, 829, 1499, 1531
Alw21I	GWGCWC	3 cut(s)	535, 876, 2112
Alw26I	GTCTC	1 cut(s)	1238
AlwNI	CAGNNNCTG	1 cut(s)	1529
AoxI	GGCC	1 cut(s)	295
ApoI	RAATTY	7 cut(s)	766, 1055, 1064, 1745, 1833, 2675, 3008
AseI	ATTAAT	1 cut(s)	2622
AsiGI	ACCGGT	1 cut(s)	527
Asp700I	GAANNNNTTC	3 cut(s)	1989, 2566, 3197
Asp718I	GGTACC	1 cut(s)	2122
AspLEI	GCGC	3 cut(s)	360, 383, 1892
AspS9I	GGNCC	1 cut(s)	110
AsuC2I	CCSGG	1 cut(s)	2246
AsuHPI	GGTGA	5 cut(s)	550, 718, 1031, 1061, 1271
AsuNHI	GCTAGC	1 cut(s)	1882
AvaII	GGWCC	1 cut(s)	110
BamHI	GGATCC	2 cut(s)	1645, 2257
BanI	GGYRCC	1 cut(s)	2122
BanII	GRGCYC	2 cut(s)	876, 2112
BauI	CACGAG	2 cut(s)	1593, 1809
BbsI	GAAGAC	1 cut(s)	897
Bbv12I	GWGCWC	3 cut(s)	535, 876, 2112
BccI	CCATC	7 cut(s)	209, 270, 1092, 2146, 2446, 2990, 3062
BceAI	ACGGC	4 cut(s)	255, 394, 661, 1706
BcgI	CGANNNNNNTGC	4 cut(s)	422, 456, 1508, 1542
BciT130I	CCWGG	2 cut(s)	803, 1099
BclI	TGATCA	1 cut(s)	2325
BcnI	CCSGG	1 cut(s)	2246
BcoDI	GTCTC	1 cut(s)	1238
BfaI	CTAG	5 cut(s)	654, 1160, 1883, 2400, 3023
BfmI	CTRYAG	2 cut(s)	1530, 2263
BfuAI	ACCTGC	1 cut(s)	1844
BglII	AGATCT	1 cut(s)	1870
BlpI	GCTNAGC	1 cut(s)	1878
Bme1390I	CCNGG	3 cut(s)	803, 1099, 2246
Bme18I	GGWCC	1 cut(s)	110
BmgBI	CACGTC	1 cut(s)	2303
BmgT120I	GGNCC	1 cut(s)	110
BmiI	GGNNCC	6 cut(s)	1227, 1647, 1986, 2124, 2158, 2259
BmrFI	CCNGG	3 cut(s)	803, 1099, 2246
BmrI	ACTGGG	2 cut(s)	2820, 3012
BmtI	GCTAGC	1 cut(s)	1886
BmuI	ACTGGG	2 cut(s)	2820, 3012
BpiI	GAAGAC	1 cut(s)	897
BpmI	CTGGAG	3 cut(s)	2168, 2229, 3249
Bpu10I	CCTNAGC	1 cut(s)	534
Bpu1102I	GCTNAGC	1 cut(s)	1878
BpuEI	CTTGAG	3 cut(s)	1434, 2084, 2597
BpuMI	CCSGG	1 cut(s)	2246
BsaBI	GATNNNNATC	2 cut(s)	1122, 1644
BsaJI	CCNNGG	4 cut(s)	1297, 1620, 2245, 2292
BsaWI	WCCGGW	3 cut(s)	527, 1580, 2933
BsaXI	ACNNNNNCTCC	6 cut(s)	1380, 1410, 2016, 2046, 2915, 2945
Bsc4I	CCNNNNNNNGG	4 cut(s)	293, 856, 1104, 2119
Bse118I	RCCGGY	1 cut(s)	527
Bse1I	ACTGG	6 cut(s)	616, 1229, 1686, 2212, 2815, 3007
Bse3DI	GCAATG	2 cut(s)	1099, 2141
Bse8I	GATNNNNATC	2 cut(s)	1122, 1644
BseBI	CCWGG	2 cut(s)	803, 1099
BseDI	CCNNGG	4 cut(s)	1297, 1620, 2245, 2292
BseGI	GGATG	7 cut(s)	201, 811, 1084, 1538, 2457, 2461, 3037
BseJI	GATNNNNATC	2 cut(s)	1122, 1644
BseLI	CCNNNNNNNGG	4 cut(s)	293, 856, 1104, 2119
BseMI	GCAATG	2 cut(s)	1099, 2141
BseNI	ACTGG	6 cut(s)	616, 1229, 1686, 2212, 2815, 3007
BseRI	GAGGAG	6 cut(s)	217, 220, 223, 280, 355, 2594
BseX3I	CGGCCG	1 cut(s)	295
BseYI	CCCAGC	1 cut(s)	451
BsgI	GTGCAG	1 cut(s)	148
Bsh1236I	CGCG	3 cut(s)	358, 381, 1890
Bsh1285I	CGRYCG	1 cut(s)	298
BshFI	GGCC	1 cut(s)	297
BshNI	GGYRCC	1 cut(s)	2122
BshTI	ACCGGT	1 cut(s)	527
BsiEI	CGRYCG	1 cut(s)	298
BsiHKAI	GWGCWC	3 cut(s)	535, 876, 2112
BsiSI	CCGG	5 cut(s)	294, 528, 1581, 2245, 2934
BslI	CCNNNNNNNGG	4 cut(s)	293, 856, 1104, 2119
BsmAI	GTCTC	1 cut(s)	1238
BsmI	GAATGC	2 cut(s)	722, 2731
BsnI	GGCC	1 cut(s)	297
Bsp1286I	GDGCHC	3 cut(s)	535, 876, 2112
Bsp1720I	GCTNAGC	1 cut(s)	1878
Bsp19I	CCATGG	2 cut(s)	1620, 2292
BspANI	GGCC	1 cut(s)	297
BspFNI	CGCG	3 cut(s)	358, 381, 1890
BspHI	TCATGA	1 cut(s)	1482
BspLI	GGNNCC	6 cut(s)	1227, 1647, 1986, 2124, 2158, 2259
BspMI	ACCTGC	1 cut(s)	1844
BspOI	GCTAGC	1 cut(s)	1886
BspQI	GCTCTTC	2 cut(s)	157, 510
BspT107I	GGYRCC	1 cut(s)	2122
BsrDI	GCAATG	2 cut(s)	1099, 2141
BsrFI	RCCGGY	1 cut(s)	527
BsrI	ACTGG	6 cut(s)	616, 1229, 1686, 2212, 2815, 3007
BssAI	RCCGGY	1 cut(s)	527
BssECI	CCNNGG	4 cut(s)	1297, 1620, 2245, 2292
BssSI	CACGAG	2 cut(s)	1593, 1809
BssT1I	CCWWGG	3 cut(s)	1297, 1620, 2292
Bst2BI	CACGAG	2 cut(s)	1593, 1809
Bst2UI	CCWGG	2 cut(s)	803, 1099
Bst4CI	ACNGT	7 cut(s)	70, 638, 690, 1013, 1073, 2333, 2927
Bst6I	CTCTTC	5 cut(s)	157, 241, 468, 510, 3165
BstAPI	GCANNNNNTGC	2 cut(s)	2303, 3224
BstC8I	GCNNGC	5 cut(s)	863, 1289, 1293, 1884, 1888
BstDSI	CCRYGG	2 cut(s)	1620, 2292
BstF5I	GGATG	7 cut(s)	201, 811, 1084, 1538, 2457, 2461, 3037
BstFNI	CGCG	3 cut(s)	358, 381, 1890
BstHHI	GCGC	3 cut(s)	360, 383, 1892
BstMAI	GTCTC	1 cut(s)	1238
BstMCI	CGRYCG	1 cut(s)	298
BstNI	CCWGG	2 cut(s)	803, 1099
BstNSI	RCATGY	2 cut(s)	1168, 1291
BstSCI	CCNGG	3 cut(s)	801, 1097, 2244
BstSFI	CTRYAG	2 cut(s)	1530, 2263
BstUI	CGCG	3 cut(s)	358, 381, 1890
BstV2I	GAAGAC	1 cut(s)	897
BstX2I	RGATCY	3 cut(s)	1645, 1870, 2257
BstYI	RGATCY	3 cut(s)	1645, 1870, 2257
BstZI	CGGCCG	1 cut(s)	295
BsuRI	GGCC	1 cut(s)	297
BtgI	CCRYGG	2 cut(s)	1620, 2292
BtgZI	GCGATG	1 cut(s)	248
BtrI	CACGTC	1 cut(s)	2303
BtsCI	GGATG	7 cut(s)	201, 811, 1084, 1538, 2457, 2461, 3037
BtsI	GCAGTG	1 cut(s)	3261
BtsIMutI	CAGTG	6 cut(s)	643, 1018, 2070, 2228, 2808, 3261
BveI	ACCTGC	1 cut(s)	1844
Cac8I	GCNNGC	5 cut(s)	863, 1289, 1293, 1884, 1888
CaiI	CAGNNNCTG	1 cut(s)	1529
CciI	TCATGA	1 cut(s)	1482
CfoI	GCGC	3 cut(s)	360, 383, 1892
Cfr10I	RCCGGY	1 cut(s)	527
Cfr13I	GGNCC	1 cut(s)	110
CseI	GACGC	1 cut(s)	1124
Csp6I	GTAC	6 cut(s)	799, 937, 1156, 1488, 2123, 2882
CspAI	ACCGGT	1 cut(s)	527
CspCI	CAANNNNNGTGG	6 cut(s)	609, 644, 810, 845, 1990, 2025
CviAII	CATG	9 cut(s)	1165, 1288, 1483, 1543, 1621, 2042, 2293, 2432, 2865
CviQI	GTAC	6 cut(s)	799, 937, 1156, 1488, 2123, 2882
DraIII	CACNNNGTG	1 cut(s)	640
EaeI	YGGCCR	1 cut(s)	295
EagI	CGGCCG	1 cut(s)	295
Eam1104I	CTCTTC	5 cut(s)	157, 241, 468, 510, 3165
EarI	CTCTTC	5 cut(s)	157, 241, 468, 510, 3165
EciI	GGCGGA	3 cut(s)	179, 296, 979
Ecl136II	GAGCTC	2 cut(s)	874, 2110
EclXI	CGGCCG	1 cut(s)	295
Eco130I	CCWWGG	3 cut(s)	1297, 1620, 2292
Eco24I	GRGCYC	2 cut(s)	876, 2112
Eco32I	GATATC	2 cut(s)	2017, 2217
Eco47I	GGWCC	1 cut(s)	110
Eco52I	CGGCCG	1 cut(s)	295
Eco53kI	GAGCTC	2 cut(s)	874, 2110
Eco57I	CTGAAG	4 cut(s)	909, 1931, 2094, 2655
EcoICRI	GAGCTC	2 cut(s)	874, 2110
EcoO109I	RGGNCCY	1 cut(s)	110
EcoRII	CCWGG	2 cut(s)	801, 1097
EcoRV	GATATC	2 cut(s)	2017, 2217
EcoT14I	CCWWGG	3 cut(s)	1297, 1620, 2292
EcoT22I	ATGCAT	1 cut(s)	724
EcoT38I	GRGCYC	2 cut(s)	876, 2112
ErhI	CCWWGG	3 cut(s)	1297, 1620, 2292
FaeI	CATG	9 cut(s)	1168, 1291, 1486, 1546, 1624, 2045, 2296, 2435, 2868
FalI	AAGNNNNNCTT	8 cut(s)	1275, 1307, 1392, 1424, 1857, 1889, 2097, 2129
FatI	CATG	9 cut(s)	1164, 1287, 1482, 1542, 1620, 2041, 2292, 2431, 2864
FauI	CCCGC	3 cut(s)	340, 1385, 2467
FauNDI	CATATG	2 cut(s)	2836, 2902
FbaI	TGATCA	1 cut(s)	2325
FblI	GTMKAC	1 cut(s)	1138
FokI	GGATG	7 cut(s)	188, 818, 1071, 1525, 2464, 2468, 3044
FriOI	GRGCYC	2 cut(s)	876, 2112
FspBI	CTAG	5 cut(s)	654, 1160, 1883, 2400, 3023
GlaI	GCGC	3 cut(s)	359, 382, 1891
GsaI	CCCAGC	1 cut(s)	455
GsuI	CTGGAG	3 cut(s)	2168, 2229, 3249
HaeIII	GGCC	1 cut(s)	297
HapII	CCGG	5 cut(s)	294, 528, 1581, 2245, 2934
HgaI	GACGC	1 cut(s)	1124
HhaI	GCGC	3 cut(s)	360, 383, 1892
Hin1II	CATG	9 cut(s)	1168, 1291, 1486, 1546, 1624, 2045, 2296, 2435, 2868
Hin6I	GCGC	3 cut(s)	358, 381, 1890
HinP1I	GCGC	3 cut(s)	358, 381, 1890
HincII	GTYRAC	1 cut(s)	3183
HindII	GTYRAC	1 cut(s)	3183
HindIII	AAGCTT	2 cut(s)	1803, 3187
HinfI	GANTC	9 cut(s)	216, 277, 456, 605, 615, 1193, 2584, 2644, 3080
HpaII	CCGG	5 cut(s)	294, 528, 1581, 2245, 2934
HphI	GGTGA	5 cut(s)	550, 718, 1031, 1061, 1271
Hpy166II	GTNNAC	8 cut(s)	799, 937, 1009, 1139, 1490, 2096, 2882, 3183
Hpy8I	GTNNAC	8 cut(s)	799, 937, 1009, 1139, 1490, 2096, 2882, 3183
Hpy99I	CGWCG	1 cut(s)	560
HpyCH4III	ACNGT	7 cut(s)	70, 638, 690, 1013, 1073, 2333, 2927
HpyCH4IV	ACGT	1 cut(s)	2302
HpySE526I	ACGT	1 cut(s)	2302
Hsp92II	CATG	9 cut(s)	1168, 1291, 1486, 1546, 1624, 2045, 2296, 2435, 2868
HspAI	GCGC	3 cut(s)	358, 381, 1890
KpnI	GGTACC	1 cut(s)	2126
Ksp22I	TGATCA	1 cut(s)	2325
LguI	GCTCTTC	2 cut(s)	157, 510
LmnI	GCTCC	5 cut(s)	230, 699, 1189, 2115, 2897
MaeI	CTAG	5 cut(s)	654, 1160, 1883, 2400, 3023
MaeII	ACGT	1 cut(s)	2302
MaeIII	GTNAC	3 cut(s)	2507, 2927, 2960
MfeI	CAATTG	2 cut(s)	3066, 3219
MflI	RGATCY	3 cut(s)	1645, 1870, 2257
MhlI	GDGCHC	3 cut(s)	535, 876, 2112
MmeI	TCCRAC	4 cut(s)	307, 576, 1151, 2764
Mph1103I	ATGCAT	1 cut(s)	724
MroXI	GAANNNNTTC	3 cut(s)	1989, 2566, 3197
MslI	CAYNNNNRTG	2 cut(s)	2869, 2934
MspA1I	CMGCKG	4 cut(s)	451, 1526, 1823, 2365
MspI	CCGG	5 cut(s)	294, 528, 1581, 2245, 2934
MspR9I	CCNGG	3 cut(s)	803, 1099, 2246
MunI	CAATTG	2 cut(s)	3066, 3219
Mva1269I	GAATGC	2 cut(s)	722, 2731
MvaI	CCWGG	2 cut(s)	803, 1099
MvnI	CGCG	3 cut(s)	358, 381, 1890
NciI	CCSGG	1 cut(s)	2246
NcoI	CCATGG	2 cut(s)	1620, 2292
NdeI	CATATG	2 cut(s)	2836, 2902
NheI	GCTAGC	1 cut(s)	1882
NlaIII	CATG	9 cut(s)	1168, 1291, 1486, 1546, 1624, 2045, 2296, 2435, 2868
NlaIV	GGNNCC	6 cut(s)	1227, 1647, 1986, 2124, 2158, 2259
NmuCI	GTSAC	2 cut(s)	2927, 2960
NsiI	ATGCAT	1 cut(s)	724
NspI	RCATGY	2 cut(s)	1168, 1291
OliI	CACNNNNGTG	1 cut(s)	2934
PaeI	GCATGC	1 cut(s)	1291
PagI	TCATGA	1 cut(s)	1482
PciI	ACATGT	1 cut(s)	1164
PciSI	GCTCTTC	2 cut(s)	157, 510
PctI	GAATGC	2 cut(s)	722, 2731
PdmI	GAANNNNTTC	3 cut(s)	1989, 2566, 3197
PfeI	GAWTC	9 cut(s)	216, 277, 456, 605, 615, 1193, 2584, 2644, 3080
PflMI	CCANNNNNTGG	1 cut(s)	1104
PinAI	ACCGGT	1 cut(s)	527
PpuMI	RGGWCCY	1 cut(s)	110
PscI	ACATGT	1 cut(s)	1164
PshBI	ATTAAT	1 cut(s)	2622
PsiI	TTATAA	1 cut(s)	903
Psp124BI	GAGCTC	2 cut(s)	876, 2112
Psp5II	RGGWCCY	1 cut(s)	110
Psp6I	CCWGG	2 cut(s)	801, 1097
PspFI	CCCAGC	1 cut(s)	451
PspGI	CCWGG	2 cut(s)	801, 1097
PspN4I	GGNNCC	6 cut(s)	1227, 1647, 1986, 2124, 2158, 2259
PspPI	GGNCC	1 cut(s)	110
PspPPI	RGGWCCY	1 cut(s)	110
PstNI	CAGNNNCTG	1 cut(s)	1529
PsuI	RGATCY	3 cut(s)	1645, 1870, 2257
PvuII	CAGCTG	4 cut(s)	451, 1526, 1823, 2365
RsaI	GTAC	6 cut(s)	800, 938, 1157, 1489, 2124, 2883
RsaNI	GTAC	6 cut(s)	799, 937, 1156, 1488, 2123, 2882
RseI	CAYNNNNRTG	2 cut(s)	2869, 2934
SacI	GAGCTC	2 cut(s)	876, 2112
SapI	GCTCTTC	2 cut(s)	157, 510
Sau96I	GGNCC	1 cut(s)	110
ScrFI	CCNGG	3 cut(s)	803, 1099, 2246
SduI	GDGCHC	3 cut(s)	535, 876, 2112
SfcI	CTRYAG	2 cut(s)	1530, 2263
SinI	GGWCC	1 cut(s)	110
SmiMI	CAYNNNNRTG	2 cut(s)	2869, 2934
SmlI	CTYRAG	3 cut(s)	1449, 2099, 2576
SmoI	CTYRAG	3 cut(s)	1449, 2099, 2576
SphI	GCATGC	1 cut(s)	1291
SspI	AATATT	3 cut(s)	1079, 1353, 1459
SspMI	CTAG	5 cut(s)	654, 1160, 1883, 2400, 3023
SstI	GAGCTC	2 cut(s)	876, 2112
StyD4I	CCNGG	3 cut(s)	801, 1097, 2244
StyI	CCWWGG	3 cut(s)	1297, 1620, 2292
TaaI	ACNGT	7 cut(s)	70, 638, 690, 1013, 1073, 2333, 2927
TaiI	ACGT	1 cut(s)	2305
TatI	WGTACW	2 cut(s)	936, 1487
TauI	GCSGC	6 cut(s)	270, 300, 312, 338, 1269, 2309
TfiI	GAWTC	9 cut(s)	216, 277, 456, 605, 615, 1193, 2584, 2644, 3080
TscAI	CASTG	6 cut(s)	643, 1018, 2077, 2235, 2815, 3261
TseFI	GTSAC	2 cut(s)	2927, 2960
Tsp45I	GTSAC	2 cut(s)	2927, 2960
TspGWI	ACGGA	1 cut(s)	1877
TspRI	CASTG	6 cut(s)	643, 1018, 2077, 2235, 2815, 3261
Van91I	CCANNNNNTGG	1 cut(s)	1104
VpaK11BI	GGWCC	1 cut(s)	110
VspI	ATTAAT	1 cut(s)	2622
XapI	RAATTY	7 cut(s)	766, 1055, 1064, 1745, 1833, 2675, 3008
XceI	RCATGY	2 cut(s)	1168, 1291
XmiI	GTMKAC	1 cut(s)	1138
XmnI	GAANNNNTTC	3 cut(s)	1989, 2566, 3197
XspI	CTAG	5 cut(s)	654, 1160, 1883, 2400, 3023
Zsp2I	ATGCAT	1 cut(s)	724

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.