Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004488

Physical Location & Seq

Genomic Coordinates Forward (+)

14012590 .. 14022296

Gene Structure 9707 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1092200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3138 bp

ATGGAGGGAGCAACTCTGCTTCGCTCTTCTCTCGCCTCCAGCAATCGCCTCTTCTTTTCCTTCCGCTCTTCCCGCCGCCTCCCCTGTTTTTCCCTCCCCTCGTCTCGCTCTTCATCTTCTTCTTCAGCTTCTGCTGTAAGAAACCACTGCCGCTGTCCGATTCCCAACCCCAGCAGACGCTCTCTGCTCCGCCCCGCCAGCAGATTACTCCCCTCCTCTGCCCCCAACGTCGCTAGAAGTTTCTCCTCCCTCTCCCCGCGCGCAGTCGCCATCCCATTCACTCAGATGAGGTCGTGGAAAAGCTCGGGTTCGAGAAAGTCTCTGAGGAGTTCATTGGAGAGTGTAAATCCAAAGCTCTGCTCTTCAGGCACAAGAAGACCGGGTGCCCAAGTAATTTCGGTGTCCAATGATGACGAGAATAAGGTTTTCGGCATTGTTTTTCGGACCCCGCCGAACGATTCCACTGGGATCCCACACATCCTTGAACACAGTGTATTGTGTGGGTCAAGAAAGTACCCCTTGAAAGAACCATTTGTTGAGTTGTTGAAAGGGAGCTTGAATACGTTTCTGAATGCATTTACTTATCCGGATAGAACTTGCTACCTGGTAGCTTCCACAAATACAAAGGATTTCTATAACCTTGTCGATGTATATCTGGATGCTGTCTTCTTTCCTAATTGTGTGGAGGACTTCAGGACTTTCCAGCAGGAGGGTTGGCATTACGAGCTCAATGATCCTTCTTACGATATATCGTATAAAGGAGTTGTTTTTAATGAGATGAAAGGGGTCTATTCCCAGCCTGATAATATACTCTGGCGGACTGCTCAACAGGCTCTTTTTCCGGACAACACCTATGCTGTTGACAGTGGGGGTGATCCAACAGTTATTCCTAAACTGACATTTGAAGAATTCAAGGAGTTCCACCGTAAGTATTACCATCCCAGCAATGCCCGGATATGGTTTTATGGAGATGATGATCCAACTGAACGTCTGCGCATTTTGAGTGAGTACCTAGATATGTTCAATGCAAGTTCATCTCCAAATGAATCAAGAATTCAACCGCAGAAACTATTTTCAGAACCAATTAGGATTTCTGAGAAATATCCTGCTGGTGAAGATGGTGACTTGAGGAAGAAAAACATGGTGTGCCTCAATTGGTTGCTTGCTGATAATCCCCTAGATTTGGAGACTGAGCTTACGCTTGGGTTTCTGGATCATCTTATGTTGGGAACGCCGGCTTCTCCATTGAGGAAAATATTGCTTGAGAGTGGGTTGGGTGATCTCCTTCAACCTCTATTTAGTATTGGTTTGAAGGGTGTTTCAGAAGATGATATTCAAAAGGTTGAAGAAGTTGTCATGAGTACACTTAAAAAGTTAGCAGAGGAAGGTTTTTATACGGAAGCTGTAGAAGCATCCATGAATACGATTGAGTTTTCCCTGAGGGAAAACAACAAGGGATCATTTCCTCGTGGCTTGTCACTAATGGTCCGATCCATAGGTAGATGGATATATGATTTGGATCCGTTTGAGCCATTAAAGTATGAGAAACCTTTGCTGGCACTGAAAGCCAGAATAGAAGCTGAAGGCTCTAAGGCTGTTTTCTCTCCCTTAATCAAGAAATTCATTCTGGACAATCCCCATCGAGTTGTGGTAGAAATGCAGCCTGATCCAGAGAAAGCTTCTCGTGATGAAGAGGCTGAGAAAGAAGTATTGCAGAAAGTTAAATCCTGTATGACAGAAGAAGATCTTGCTGAGCTAGCACGTGCTACACAGGAGCTGCAACTGCGGCAGGAAACTCCTGACCTTCCGGAAGCTTTGACAAGTGTTCCAAGTCTCTCTTTGCAAGACATTTCAAGGGAACCTACTCGTATTCCTACAGTTGGGGATATCAATGGCGCGAAAGTTCTGCGGCATGACCTCTTCACAAATGCTGTCCTGTACATTGAAGTTGTGTTCAATATGAGTTCACTAAAGCAAGAGCTACTTCCTTTGGTACCTCTTTTTTGCCAATCGTTGCTAGAGATGGGTACAAAAGATTTGAGTTTTGTGCAACTGAATCAATTGACCGGAAGGAAAACTGGAGGAATATCAGTTTATCCAATGACATCATCTGTACATGGCAAGGCGGATCCCTGCAGCCATATAATTGTTCGTGGAAAAGCTATGGCAGGACGTGCTGATGATCTATTTCACCTGTTCAATTGTGTTCTTCAAGAAGTCCAGTTTACAGACCAGCAGCGGTTTAAGCAGTTTGTCTCCCAAAGCAAAGCGAGAATGGAGAACCGATTGAGAGGTGGTGGTCATGGAATTGCTGCTTCAAGGATATGCAAAGCTAAATACTGTGGGGTGGATTTCAGAACAAATGGGTGGTGTCAGTTTCTTCAAGCTCTTGAAGAGAAAGTTGATCAAGATTGGGATGGAATTTCTTCATCTCTTGAGGAGATCCGCAAGTCCTTGCTTTCTAGGCAAGGTTCCATAGTAAATATCACTGCTGAAGGGAAGAATCTCACCAACTCTGAGAAGTTCATGAGCAAATTTCTTGATTTGCTGCCTAACAGCTCTCCTGTTGCGACTGCTTCTTGGAATGCTCAATTACCTTCAGCAAATGAAGCCATTGTAATCCCTACTCAGGTTAATTATGTTGGAAAGGCTGGTAACATTTATGACGCTGGTTATCGGCTTAGTGGGAGTGCACATGTTATCTCCAAATACATTAGTAATACATGGTTGTGGGATCGTGTAAGAGTTAGTGGTGGAGCATATGGAGGATTTTGTGACTTTGATAGTCACTCAGGAGTATTCTCCTTCTTATCTTATCGGGACCCCAACTTGTTAAAGACACTTGGTACTCTTTCCAAGTCCATCATTGGGACAATCGGAGATGTTGATTCGTACCAGCTTCCTGATGCCAAAGGTTATAGTAGTTTGTTGCGATATTTATTGGGAATCACAGAAGAAGAAAGGCAAGTAAGACGAGCAGAGATACTGTCAACAAGCTTGAAGGACTTCAAGGAATTTGCAAATGCGATCGACGTAGTCAAAGACAAAGGGGTCGTGGTTGCAGTGGCATCTCCAGATGACGTCGATGCAGCACAAAAGGAGCGAAACGACTTCTTTCAAGTAAAAAAAGCCCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

1046

Amino Acids

116.87

Weight (kDa)

6.62

Isoelectric Point (pI)

47.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	151 - 225	3.8e-06	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	298 - 474	2.3e-22	Peptidase M16 inactive domain
M16C_assoc	PF08367	553 - 775	4.3e-64	Peptidase M16C associated
PreP_C	PF22516	869 - 1024	4.2e-65	PreP C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT1G49630 AT3G19170 AT3G19170
fragaria_vesca	FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380 FvH4_2g06380
malus_domestica	MD05G1078000.v1.1 MD10G1092200.v1.1 MD11G1150900.v1.1
prunus_persica	Prupe.8G122100_v2.0.a1 Prupe.8G122200_v2.0.a1
pyrus_communis	pycom05g07050 pycom05g07160 pycom10g07580
rosa_chinensis	RchiOBHm_Chr6g0258261
rosa_laevigata	RLG00000014634
rosa_multiflora	Rmu_sc0004230.1_g000015
rosa_roxburghii	Rroxscaffold_178G00437220 Rroxscaffold_7G00205380
rosa_rugosa	Rorug05G0579700
rosa_samantha	Rh6AG097600 Rh6BG088700 Rh6CG084700 Rh6DG080800
rosa_wichuraiana	Rw6G008290 Rw6G008350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3050
AatII	GACGTC	1 cut(s)	3084
Acc16I	TGCGCA	1 cut(s)	995
Acc65I	GGTACC	1 cut(s)	1993
AccB1I	GGYRCC	2 cut(s)	383, 1993
AccBSI	CCGCTC	1 cut(s)	66
AccII	CGCG	3 cut(s)	259, 261, 1898
AccIII	TCCGGA	3 cut(s)	586, 841, 1807
AcsI	RAATTY	6 cut(s)	908, 1053, 1619, 2421, 2534, 3014
AcuI	CTGAAG	6 cut(s)	108, 348, 676, 1602, 2514, 2583
AcvI	CACGTG	1 cut(s)	1763
AcyI	GRCGYC	1 cut(s)	3081
AfaI	GTAC	9 cut(s)	515, 1010, 1363, 1940, 1995, 2029, 2115, 2848, 2894
AfiI	CCNNNNNNNGG	6 cut(s)	709, 957, 1183, 1880, 2629, 2868
AflIII	ACRYGT	1 cut(s)	2695
AjiI	CACGTC	1 cut(s)	2174
AjnI	CCWGG	1 cut(s)	603
AjuI	GAANNNNNNNTTGG	2 cut(s)	1075, 1107
Alw21I	GWGCWC	2 cut(s)	729, 2695
Alw26I	GTCTC	5 cut(s)	108, 324, 1181, 1838, 2260
Alw44I	GTGCAC	1 cut(s)	2691
AlwNI	CAGNNNCTG	2 cut(s)	131, 1777
Ama87I	CYCGRG	1 cut(s)	304
Aor13HI	TCCGGA	3 cut(s)	586, 841, 1807
ApaLI	GTGCAC	1 cut(s)	2691
ApeKI	GCWGC	7 cut(s)	1660, 1777, 2136, 2236, 2312, 2548, 3089
ApoI	RAATTY	6 cut(s)	908, 1053, 1619, 2421, 2534, 3014
Asp700I	GAANNNNTTC	2 cut(s)	2425, 3005
Asp718I	GGTACC	1 cut(s)	1993
AspLEI	GCGC	4 cut(s)	261, 263, 996, 1898
AspS9I	GGNCC	3 cut(s)	444, 1486, 2821
AsuC2I	CCSGG	2 cut(s)	381, 952
AsuHPI	GGTGA	6 cut(s)	884, 1124, 1133, 1289, 2183, 2500
AsuNHI	GCTAGC	1 cut(s)	1756
AvaI	CYCGRG	1 cut(s)	304
AvaII	GGWCC	3 cut(s)	444, 1486, 2821
AxyI	CCTNAGG	1 cut(s)	1439
BaeGI	GKGCMC	2 cut(s)	388, 2695
BamHI	GGATCC	3 cut(s)	468, 1519, 2128
BanI	GGYRCC	2 cut(s)	383, 1993
BanII	GRGCYC	1 cut(s)	729
BauI	CACGAG	2 cut(s)	1467, 1683
BbrPI	CACGTG	1 cut(s)	1763
BbsI	GAAGAC	2 cut(s)	382, 658
Bbv12I	GWGCWC	2 cut(s)	729, 2695
BbvI	GCAGC	7 cut(s)	1672, 1764, 2148, 2248, 2299, 2535, 3101
BccI	CCATC	8 cut(s)	278, 945, 1112, 1497, 1647, 2017, 2411, 2870
BcgI	CGANNNNNNTGC	2 cut(s)	1888, 1922
BciT130I	CCWGG	1 cut(s)	605
BclI	TGATCA	1 cut(s)	2404
BcnI	CCSGG	2 cut(s)	381, 952
BcoDI	GTCTC	5 cut(s)	108, 324, 1181, 1838, 2260
BfaI	CTAG	7 cut(s)	234, 1013, 1178, 1757, 2018, 2463, 3136
BfmI	CTRYAG	3 cut(s)	1404, 1875, 2134
BglII	AGATCT	1 cut(s)	1744
BlpI	GCTNAGC	1 cut(s)	1752
Bme1390I	CCNGG	3 cut(s)	381, 605, 952
Bme18I	GGWCC	3 cut(s)	444, 1486, 2821
BmeT110I	CYCGRG	1 cut(s)	304
BmgBI	CACGTC	1 cut(s)	2174
BmgT120I	GGNCC	3 cut(s)	444, 1486, 2821
BmrFI	CCNGG	3 cut(s)	381, 605, 952
BmrI	ACTGGG	1 cut(s)	474
BmsI	GCATC	5 cut(s)	649, 1421, 2896, 3076, 3077
BmtI	GCTAGC	1 cut(s)	1760
BmuI	ACTGGG	1 cut(s)	474
BpiI	GAAGAC	2 cut(s)	382, 658
BplI	GAGNNNNNCTC	2 cut(s)	304, 336
BpmI	CTGGAG	3 cut(s)	22, 2100, 3057
Bpu1102I	GCTNAGC	1 cut(s)	1752
BpuEI	CTTGAG	3 cut(s)	1147, 1283, 2456
BpuMI	CCSGG	2 cut(s)	381, 952
BsaAI	YACGTR	1 cut(s)	1763
BsaBI	GATNNNNATC	3 cut(s)	651, 975, 1518
BsaHI	GRCGYC	1 cut(s)	3081
BsaWI	WCCGGW	4 cut(s)	586, 841, 1807, 2066
Bsc4I	CCNNNNNNNGG	6 cut(s)	709, 957, 1183, 1880, 2629, 2868
Bse118I	RCCGGY	1 cut(s)	1234
Bse1I	ACTGG	3 cut(s)	469, 2083, 2221
Bse21I	CCTNAGG	1 cut(s)	1439
Bse3DI	GCAATG	1 cut(s)	952
Bse8I	GATNNNNATC	3 cut(s)	651, 975, 1518
BseAI	TCCGGA	3 cut(s)	586, 841, 1807
BseBI	CCWGG	1 cut(s)	605
BseGI	GGATG	6 cut(s)	270, 477, 664, 937, 1412, 2422
BseJI	GATNNNNATC	3 cut(s)	651, 975, 1518
BseLI	CCNNNNNNNGG	6 cut(s)	709, 957, 1183, 1880, 2629, 2868
BseMI	GCAATG	1 cut(s)	952
BseNI	ACTGG	3 cut(s)	469, 2083, 2221
BsePI	GCGCGC	1 cut(s)	259
BseRI	GAGGAG	4 cut(s)	205, 235, 340, 2453
BseSI	GKGCMC	2 cut(s)	388, 2695
BseXI	GCAGC	7 cut(s)	1672, 1764, 2148, 2248, 2299, 2535, 3101
BseYI	CCCAGC	3 cut(s)	170, 795, 941
Bsh1236I	CGCG	3 cut(s)	259, 261, 1898
Bsh1285I	CGRYCG	1 cut(s)	3030
BshNI	GGYRCC	2 cut(s)	383, 1993
BsiEI	CGRYCG	1 cut(s)	3030
BsiHKAI	GWGCWC	2 cut(s)	729, 2695
BsiHKCI	CYCGRG	1 cut(s)	304
BsiSI	CCGG	7 cut(s)	380, 587, 842, 952, 1235, 1808, 2067
BslFI	GGGAC	2 cut(s)	2834, 2884
BslI	CCNNNNNNNGG	6 cut(s)	709, 957, 1183, 1880, 2629, 2868
BsmAI	GTCTC	5 cut(s)	108, 324, 1181, 1838, 2260
BsmBI	CGTCTC	1 cut(s)	108
BsmFI	GGGAC	2 cut(s)	2834, 2884
BsmI	GAATGC	2 cut(s)	577, 2590
BsoBI	CYCGRG	1 cut(s)	304
Bsp1286I	GDGCHC	3 cut(s)	388, 729, 2695
Bsp13I	TCCGGA	3 cut(s)	586, 841, 1807
Bsp1407I	TGTACA	2 cut(s)	1938, 2113
Bsp1720I	GCTNAGC	1 cut(s)	1752
BspEI	TCCGGA	3 cut(s)	586, 841, 1807
BspFNI	CGCG	3 cut(s)	259, 261, 1898
BspHI	TCATGA	2 cut(s)	1356, 2526
BspMAI	CTGCAG	1 cut(s)	2138
BspOI	GCTAGC	1 cut(s)	1760
BspQI	GCTCTTC	4 cut(s)	31, 73, 115, 367
BspT107I	GGYRCC	2 cut(s)	383, 1993
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	1 cut(s)	952
BsrFI	RCCGGY	1 cut(s)	1234
BsrGI	TGTACA	2 cut(s)	1938, 2113
BsrI	ACTGG	3 cut(s)	469, 2083, 2221
BssAI	RCCGGY	1 cut(s)	1234
BssHII	GCGCGC	1 cut(s)	259
BssNI	GRCGYC	1 cut(s)	3081
BssSI	CACGAG	2 cut(s)	1467, 1683
Bst2BI	CACGAG	2 cut(s)	1467, 1683
Bst2UI	CCWGG	1 cut(s)	605
Bst4CI	ACNGT	7 cut(s)	491, 866, 883, 926, 1879, 2342, 2988
Bst6I	CTCTTC	8 cut(s)	31, 56, 73, 115, 367, 1686, 1925, 2388
BstACI	GRCGYC	1 cut(s)	3081
BstAPI	GCANNNNNTGC	1 cut(s)	2174
BstAUI	TGTACA	2 cut(s)	1938, 2113
BstBAI	YACGTR	1 cut(s)	1763
BstC8I	GCNNGC	6 cut(s)	199, 261, 1164, 1236, 1557, 1758
BstF5I	GGATG	6 cut(s)	270, 477, 664, 937, 1412, 2422
BstFNI	CGCG	3 cut(s)	259, 261, 1898
BstHHI	GCGC	4 cut(s)	261, 263, 996, 1898
BstMAI	GTCTC	5 cut(s)	108, 324, 1181, 1838, 2260
BstMCI	CGRYCG	1 cut(s)	3030
BstNI	CCWGG	1 cut(s)	605
BstNSI	RCATGY	1 cut(s)	2699
BstSCI	CCNGG	3 cut(s)	379, 603, 950
BstSFI	CTRYAG	3 cut(s)	1404, 1875, 2134
BstSLI	GKGCMC	2 cut(s)	388, 2695
BstUI	CGCG	3 cut(s)	259, 261, 1898
BstV1I	GCAGC	7 cut(s)	1672, 1764, 2148, 2248, 2299, 2535, 3101
BstV2I	GAAGAC	2 cut(s)	382, 658
BstX2I	RGATCY	5 cut(s)	468, 1519, 1744, 2128, 2442
BstYI	RGATCY	5 cut(s)	468, 1519, 1744, 2128, 2442
Bsu36I	CCTNAGG	1 cut(s)	1439
BtrI	CACGTC	1 cut(s)	2174
BtsCI	GGATG	6 cut(s)	270, 477, 664, 937, 1412, 2422
BtsI	GCAGTG	3 cut(s)	145, 2487, 3069
BtsIMutI	CAGTG	7 cut(s)	145, 462, 496, 871, 1559, 2487, 3069
Cac8I	GCNNGC	6 cut(s)	199, 261, 1164, 1236, 1557, 1758
CaiI	CAGNNNCTG	2 cut(s)	131, 1777
CciI	TCATGA	2 cut(s)	1356, 2526
CfoI	GCGC	4 cut(s)	261, 263, 996, 1898
Cfr10I	RCCGGY	1 cut(s)	1234
Cfr13I	GGNCC	3 cut(s)	444, 1486, 2821
CseI	GACGC	2 cut(s)	186, 2675
CsiI	ACCWGGT	1 cut(s)	603
Csp6I	GTAC	9 cut(s)	514, 1009, 1362, 1939, 1994, 2028, 2114, 2847, 2893
CviAII	CATG	9 cut(s)	1141, 1357, 1417, 1913, 2117, 2303, 2527, 2696, 2724
CviQI	GTAC	9 cut(s)	514, 1009, 1362, 1939, 1994, 2028, 2114, 2847, 2893
DrdI	GACNNNNNNGTC	1 cut(s)	3050
DseDI	GACNNNNNNGTC	1 cut(s)	3050
Eam1104I	CTCTTC	8 cut(s)	31, 56, 73, 115, 367, 1686, 1925, 2388
EarI	CTCTTC	8 cut(s)	31, 56, 73, 115, 367, 1686, 1925, 2388
EciI	GGCGGA	3 cut(s)	179, 832, 2141
Ecl136II	GAGCTC	1 cut(s)	727
Eco24I	GRGCYC	1 cut(s)	729
Eco32I	GATATC	1 cut(s)	1888
Eco47I	GGWCC	3 cut(s)	444, 1486, 2821
Eco53kI	GAGCTC	1 cut(s)	727
Eco57I	CTGAAG	6 cut(s)	108, 348, 676, 1602, 2514, 2583
Eco72I	CACGTG	1 cut(s)	1763
Eco81I	CCTNAGG	1 cut(s)	1439
Eco88I	CYCGRG	1 cut(s)	304
EcoICRI	GAGCTC	1 cut(s)	727
EcoO109I	RGGNCCY	1 cut(s)	2821
EcoRI	GAATTC	2 cut(s)	908, 1053
EcoRII	CCWGG	1 cut(s)	603
EcoRV	GATATC	1 cut(s)	1888
EcoT22I	ATGCAT	1 cut(s)	577
EcoT38I	GRGCYC	1 cut(s)	729
Esp3I	CGTCTC	1 cut(s)	108
FaeI	CATG	9 cut(s)	1144, 1360, 1420, 1916, 2120, 2306, 2530, 2699, 2727
FalI	AAGNNNNNCTT	8 cut(s)	503, 535, 1731, 1763, 1822, 1854, 1968, 2000
FaqI	GGGAC	2 cut(s)	2834, 2884
FatI	CATG	9 cut(s)	1140, 1356, 1416, 1912, 2116, 2302, 2526, 2695, 2723
FauI	CCCGC	4 cut(s)	80, 202, 264, 456
FauNDI	CATATG	1 cut(s)	2761
FbaI	TGATCA	1 cut(s)	2404
FokI	GGATG	6 cut(s)	257, 464, 671, 924, 1399, 2429
FriOI	GRGCYC	1 cut(s)	729
FspBI	CTAG	7 cut(s)	234, 1013, 1178, 1757, 2018, 2463, 3136
FspI	TGCGCA	1 cut(s)	995
GlaI	GCGC	4 cut(s)	260, 262, 995, 1897
GsaI	CCCAGC	3 cut(s)	174, 799, 945
GsuI	CTGGAG	3 cut(s)	22, 2100, 3057
HapII	CCGG	7 cut(s)	380, 587, 842, 952, 1235, 1808, 2067
HgaI	GACGC	2 cut(s)	186, 2675
HhaI	GCGC	4 cut(s)	261, 263, 996, 1898
Hin1I	GRCGYC	1 cut(s)	3081
Hin1II	CATG	9 cut(s)	1144, 1360, 1420, 1916, 2120, 2306, 2530, 2699, 2727
Hin6I	GCGC	4 cut(s)	259, 261, 994, 1896
HinP1I	GCGC	4 cut(s)	259, 261, 994, 1896
HincII	GTYRAC	2 cut(s)	862, 2991
HindII	GTYRAC	2 cut(s)	862, 2991
HindIII	AAGCTT	3 cut(s)	1677, 1812, 2995
HinfI	GANTC	7 cut(s)	160, 458, 1046, 2056, 2503, 2888, 2946
HpaII	CCGG	7 cut(s)	380, 587, 842, 952, 1235, 1808, 2067
HphI	GGTGA	6 cut(s)	884, 1124, 1133, 1289, 2183, 2500
Hpy166II	GTNNAC	6 cut(s)	862, 1364, 1967, 2226, 2693, 2991
Hpy8I	GTNNAC	6 cut(s)	862, 1364, 1967, 2226, 2693, 2991
Hpy99I	CGWCG	3 cut(s)	233, 3035, 3086
HpyCH4III	ACNGT	7 cut(s)	491, 866, 883, 926, 1879, 2342, 2988
HpyCH4IV	ACGT	7 cut(s)	228, 563, 988, 1762, 2173, 3033, 3081
HpySE526I	ACGT	7 cut(s)	228, 563, 988, 1762, 2173, 3033, 3081
Hsp92I	GRCGYC	1 cut(s)	3081
Hsp92II	CATG	9 cut(s)	1144, 1360, 1420, 1916, 2120, 2306, 2530, 2699, 2727
HspAI	GCGC	4 cut(s)	259, 261, 994, 1896
KflI	GGGWCCC	1 cut(s)	2821
Kpn2I	TCCGGA	3 cut(s)	586, 841, 1807
KpnI	GGTACC	1 cut(s)	1997
KroI	GCCGGC	1 cut(s)	1234
KroNI	GCCGGC	1 cut(s)	1236
Ksp22I	TGATCA	1 cut(s)	2404
LguI	GCTCTTC	4 cut(s)	31, 73, 115, 367
LmnI	GCTCC	6 cut(s)	8, 192, 552, 1774, 2756, 3100
Lsp1109I	GCAGC	7 cut(s)	1672, 1764, 2148, 2248, 2299, 2535, 3101
LweI	GCATC	5 cut(s)	649, 1421, 2896, 3076, 3077
MabI	ACCWGGT	1 cut(s)	603
MaeI	CTAG	7 cut(s)	234, 1013, 1178, 1757, 2018, 2463, 3136
MaeII	ACGT	7 cut(s)	228, 563, 988, 1762, 2173, 3033, 3081
MaeIII	GTNAC	5 cut(s)	1121, 1476, 2654, 2774, 2786
MbiI	CCGCTC	1 cut(s)	66
MfeI	CAATTG	3 cut(s)	1153, 2060, 2200
MflI	RGATCY	5 cut(s)	468, 1519, 1744, 2128, 2442
MhlI	GDGCHC	3 cut(s)	388, 729, 2695
MmeI	TCCRAC	3 cut(s)	902, 1004, 2623
Mph1103I	ATGCAT	1 cut(s)	577
MroI	TCCGGA	3 cut(s)	586, 841, 1807
MroNI	GCCGGC	1 cut(s)	1234
MroXI	GAANNNNTTC	2 cut(s)	2425, 3005
MseI	TTAA	8 cut(s)	771, 1368, 1535, 1610, 1722, 2244, 2634, 2834
MslI	CAYNNNNRTG	2 cut(s)	284, 2728
MspA1I	CMGCKG	2 cut(s)	153, 2239
MspI	CCGG	7 cut(s)	380, 587, 842, 952, 1235, 1808, 2067
MspR9I	CCNGG	3 cut(s)	381, 605, 952
MunI	CAATTG	3 cut(s)	1153, 2060, 2200
Mva1269I	GAATGC	2 cut(s)	577, 2590
MvaI	CCWGG	1 cut(s)	605
MvnI	CGCG	3 cut(s)	259, 261, 1898
NaeI	GCCGGC	1 cut(s)	1236
NciI	CCSGG	2 cut(s)	381, 952
NdeI	CATATG	1 cut(s)	2761
NgoMIV	GCCGGC	1 cut(s)	1234
NheI	GCTAGC	1 cut(s)	1756
NlaIII	CATG	9 cut(s)	1144, 1360, 1420, 1916, 2120, 2306, 2530, 2699, 2727
NmuCI	GTSAC	4 cut(s)	1121, 1476, 2774, 2786
NsbI	TGCGCA	1 cut(s)	995
NsiI	ATGCAT	1 cut(s)	577
NspI	RCATGY	1 cut(s)	2699
PagI	TCATGA	2 cut(s)	1356, 2526
PauI	GCGCGC	1 cut(s)	259
PciI	ACATGT	1 cut(s)	2695
PciSI	GCTCTTC	4 cut(s)	31, 73, 115, 367
PctI	GAATGC	2 cut(s)	577, 2590
PdiI	GCCGGC	1 cut(s)	1236
PdmI	GAANNNNTTC	2 cut(s)	2425, 3005
PfeI	GAWTC	7 cut(s)	160, 458, 1046, 2056, 2503, 2888, 2946
PflFI	GACNNNGTC	1 cut(s)	3035
Ple19I	CGATCG	1 cut(s)	3030
PmaCI	CACGTG	1 cut(s)	1763
PmlI	CACGTG	1 cut(s)	1763
Ppu21I	YACGTR	1 cut(s)	1763
PpuMI	RGGWCCY	1 cut(s)	2821
PscI	ACATGT	1 cut(s)	2695
Psp124BI	GAGCTC	1 cut(s)	729
Psp5II	RGGWCCY	1 cut(s)	2821
Psp6I	CCWGG	1 cut(s)	603
PspCI	CACGTG	1 cut(s)	1763
PspFI	CCCAGC	3 cut(s)	170, 795, 941
PspGI	CCWGG	1 cut(s)	603
PspPI	GGNCC	3 cut(s)	444, 1486, 2821
PspPPI	RGGWCCY	1 cut(s)	2821
PstI	CTGCAG	1 cut(s)	2138
PstNI	CAGNNNCTG	2 cut(s)	131, 1777
PsuI	RGATCY	5 cut(s)	468, 1519, 1744, 2128, 2442
PsyI	GACNNNGTC	1 cut(s)	3035
PteI	GCGCGC	1 cut(s)	259
PvuI	CGATCG	1 cut(s)	3030
RsaI	GTAC	9 cut(s)	515, 1010, 1363, 1940, 1995, 2029, 2115, 2848, 2894
RsaNI	GTAC	9 cut(s)	514, 1009, 1362, 1939, 1994, 2028, 2114, 2847, 2893
RseI	CAYNNNNRTG	2 cut(s)	284, 2728
SacI	GAGCTC	1 cut(s)	729
SapI	GCTCTTC	4 cut(s)	31, 73, 115, 367
SaqAI	TTAA	8 cut(s)	771, 1368, 1535, 1610, 1722, 2244, 2634, 2834
Sau96I	GGNCC	3 cut(s)	444, 1486, 2821
ScrFI	CCNGG	3 cut(s)	381, 605, 952
SduI	GDGCHC	3 cut(s)	388, 729, 2695
SexAI	ACCWGGT	1 cut(s)	603
SfaNI	GCATC	5 cut(s)	649, 1421, 2896, 3076, 3077
SfcI	CTRYAG	3 cut(s)	1404, 1875, 2134
SinI	GGWCC	3 cut(s)	444, 1486, 2821
SmiMI	CAYNNNNRTG	2 cut(s)	284, 2728
SmlI	CTYRAG	3 cut(s)	1126, 1262, 2435
SmoI	CTYRAG	3 cut(s)	1126, 1262, 2435
SspI	AATATT	1 cut(s)	1257
SspMI	CTAG	7 cut(s)	234, 1013, 1178, 1757, 2018, 2463, 3136
SstI	GAGCTC	1 cut(s)	729
StyD4I	CCNGG	3 cut(s)	379, 603, 950
TaaI	ACNGT	7 cut(s)	491, 866, 883, 926, 1879, 2342, 2988
TaiI	ACGT	7 cut(s)	231, 566, 991, 1765, 2176, 3036, 3084
TaqI	TCGA	5 cut(s)	311, 645, 1642, 3030, 3084
TatI	WGTACW	3 cut(s)	1361, 1938, 2113
TauI	GCSGC	4 cut(s)	78, 153, 1789, 1912
TfiI	GAWTC	7 cut(s)	160, 458, 1046, 2056, 2503, 2888, 2946
Tru1I	TTAA	8 cut(s)	771, 1368, 1535, 1610, 1722, 2244, 2634, 2834
Tru9I	TTAA	8 cut(s)	771, 1368, 1535, 1610, 1722, 2244, 2634, 2834
TscAI	CASTG	7 cut(s)	152, 469, 496, 871, 1566, 2494, 3069
TseFI	GTSAC	4 cut(s)	1121, 1476, 2774, 2786
TseI	GCWGC	7 cut(s)	1660, 1777, 2136, 2236, 2312, 2548, 3089
Tsp45I	GTSAC	4 cut(s)	1121, 1476, 2774, 2786
TspGWI	ACGGA	2 cut(s)	1412, 1512
TspRI	CASTG	7 cut(s)	152, 469, 496, 871, 1566, 2494, 3069
Tth111I	GACNNNGTC	1 cut(s)	3035
VneI	GTGCAC	1 cut(s)	2691
VpaK11BI	GGWCC	3 cut(s)	444, 1486, 2821
XapI	RAATTY	6 cut(s)	908, 1053, 1619, 2421, 2534, 3014
XceI	RCATGY	1 cut(s)	2699
XmnI	GAANNNNTTC	2 cut(s)	2425, 3005
XspI	CTAG	7 cut(s)	234, 1013, 1178, 1757, 2018, 2463, 3136
ZraI	GACGTC	1 cut(s)	3082
Zsp2I	ATGCAT	1 cut(s)	577

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.