AT3G20050
TCP Family

assists the folding of proteins upon ATP hydrolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010305
Physical Location & Seq
Reverse (-)
6998159 .. 7002435
4277 bp
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UTR
Exon/CDS
Intron
AT3G20050.1

Sequence Viewer

Length: 1638 bp
ATGTCGATCTCCGCCCAAAATCCGGATATTTCCGGCGATAGACAATCCGGCCAAGATGTTCGTACTCAGAACGTGATGGCTTGTCAAGCTGTTTCTAATATTGTTAAGACCTCACTTGGTCCCGTCGGCCTCGACAAGATGTTGGTGGATGATATTGGTGATGTGACTATTACAAATGATGGTGCTACTATTCTTAGGATGTTGGAGGTCGAGCACCCTGCTGCTAAGGTTCTTGTCGAGTTAGCTGAACTTCAAGATAGAGAAGTCGGTGATGGGACTACATCTGTTGTTATTGTAGCTGCTGAGTTGTTGAAGAGGGCAAATGATTTGGTCAGGAACAAAATACATCCAACATCAATTATCAGTGGATATAGGCTAGCAATGAGAGAATCTTGCAAATACATAGAAGAGAAGTTGGTGACCAAGGTCGAAAAGCTTGGGAAAGTTCCATTGATAAACTGTGCCAAAACCAGCATGTCCTCCAAACTGATATCTGGTGACAGCGACTTCTTTGCCAATTTGGTTGTGGAAGCCGTTCTTTCAGTAAAGATGACAAATCAGCGGGGAGAGATAAAATACCCTATCAAGGGCATTAATATTCTGAAAGCTCATGGACAAAGTGCAAGAGACAGCTATTTGTTAAATGGATATGCACTCAATACTGGTCGTGCTGCCCAAGGGATGCCATTACGAGTGTCCCCAGCAAAGATTGCTTGCCTTGACTTCAATCTGCAGAAGACAAAAATGCAGCTGGGTGTCCAAGTTGTTGTTAATGACCCACGAGAACTTGAAAAGATCCGTCAAAGAGAAGCTGACATGACAAAAGAGCGGATAGAGAAACTTCTCAAAGCTGGGGCCAATGTAATTCTGACCACAAAGGGGATTGACGACATGGCACTTAAATACTTTGTAGAGGCAGGAGCTATTGCTGTTAGGCGTGTGCGCAAAGAAGACATGCGTCATGTTGCCAAGGCGACAGGAGCGACTCTGGTCACCACTTTTGCTGACATGGAGGGCGAAGAAACATTTGATCCAGCTCACCTTGGATCTGCTGATGAGGTTGTTGAAGAGAGAATCGCAGATGATGATGTTATATTGATAAAGGGAACCAAAACAAGTAGTGCGGTCTCGTTGATCTTAAGAGGTGCAAATGACTACATGCTTGATGAGATGGAGAGAGCACTGCATGACGCTTTATGTATTGTCAAGAGGACCCTTGAGTCCAACACGGTGGTTGCGGGTGGAGGTGCAGTTGAATCAGCATTGTCTGTGTATCTGGAGCACCTTGCTACAACCTTGGGCTCTCGCGAACAATTGGCCATCGCTGAATTTGCTGATGCGCTATTGATTATACCAAAGGTGCTTGCAGTAAACGCTGCTAAGGATGCAACCGAATTGGTAGCTAAACTAAGAGCGTACCACCACACTGCACAAACCAAGGCTGATAAGAAGCACTATTCAAGCATGGGACTTGACCTCGTAAACGGAACCATCCGCAACAATTTGGAAGCTGGAGTGATCGAACCAGCTATGAGCAAAGTGAAAATTATCCAGTTTGCAACAGAGGCAGCCATAACGATTCTACGAATTGACGACATGATCAAACTGGTGAAGGATGAGAGCCAAGGCGAAGAATAA

Protein Analysis

545

Amino Acids

59.23

Weight (kDa)

5.93

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 31 - 535 6.4e-160 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1219
Acc16I TGCGCA 1 cut(s) 944
AccBSI CCGCTC 1 cut(s) 829
AccII CGCG 1 cut(s) 1308
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 6 cut(s) 12, 562, 829, 1124, 1238, 1495
AclWI GGATC 3 cut(s) 790, 1025, 1054
AcoI YGGCCR 2 cut(s) 49, 1317
AcsI RAATTY 1 cut(s) 1328
AfaI GTAC 2 cut(s) 64, 1418
AfiI CCNNNNNNNGG 4 cut(s) 22, 586, 587, 879
AflII CTTAAG 1 cut(s) 1138
AgsI TTSAA 7 cut(s) 254, 313, 727, 791, 1067, 1256, 1461
Alw21I GWGCWC 3 cut(s) 216, 1183, 1284
Alw26I GTCTC 2 cut(s) 621, 1132
AlwI GGATC 3 cut(s) 790, 1025, 1054
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 4 cut(s) 49, 127, 855, 1317
ApeKI GCWGC 6 cut(s) 221, 299, 671, 748, 1376, 1568
ApoI RAATTY 1 cut(s) 1328
AseI ATTAAT 1 cut(s) 594
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 2 cut(s) 945, 1342
AspS9I GGNCC 3 cut(s) 119, 855, 1212
AsuHPI GGTGA 7 cut(s) 170, 281, 430, 509, 985, 1031, 1621
AsuNHI GCTAGC 1 cut(s) 376
AvaII GGWCC 2 cut(s) 119, 1212
BalI TGGCCA 1 cut(s) 1319
BanII GRGCYC 1 cut(s) 1304
BauI CACGAG 1 cut(s) 780
BbsI GAAGAC 2 cut(s) 743, 957
Bbv12I GWGCWC 3 cut(s) 216, 1183, 1284
BbvI GCAGC 6 cut(s) 208, 286, 658, 760, 1363, 1580
BccI CCATC 6 cut(s) 70, 173, 266, 1165, 1328, 1499
BceAI ACGGC 1 cut(s) 518
BcgI CGANNNNNNTGC 4 cut(s) 200, 234, 494, 528
BclI TGATCA 1 cut(s) 1599
BcoDI GTCTC 2 cut(s) 621, 1132
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 731
BfrI CTTAAG 1 cut(s) 1138
BisI GCNGC 6 cut(s) 222, 300, 672, 749, 1377, 1569
BlsI GCNGC 6 cut(s) 223, 301, 673, 750, 1378, 1570
Bme18I GGWCC 2 cut(s) 119, 1212
BmgT120I GGNCC 3 cut(s) 119, 855, 1212
BmiI GGNNCC 5 cut(s) 121, 856, 1108, 1214, 1489
BmsI GCATC 3 cut(s) 672, 1327, 1375
BmtI GCTAGC 1 cut(s) 380
BoxI GACNNNNGTC 3 cut(s) 425, 957, 989
BpiI GAAGAC 2 cut(s) 743, 957
BpmI CTGGAG 2 cut(s) 1298, 1533
Bpu10I CCTNAGC 2 cut(s) 225, 1380
BpuEI CTTGAG 1 cut(s) 1238
BsaI GGTCTC 1 cut(s) 1132
BsaJI CCNNGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
BsaWI WCCGGW 1 cut(s) 22
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 586, 587, 879
Bse1I ACTGG 3 cut(s) 667, 1552, 1611
Bse3DI GCAATG 1 cut(s) 387
BseAI TCCGGA 1 cut(s) 22
BseDI CCNNGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
BseGI GGATG 7 cut(s) 154, 204, 346, 687, 1390, 1491, 1621
BseLI CCNNNNNNNGG 4 cut(s) 22, 586, 587, 879
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 80, 294
BseNI ACTGG 3 cut(s) 667, 1552, 1611
BseXI GCAGC 6 cut(s) 208, 286, 658, 760, 1363, 1580
BseYI CCCAGC 3 cut(s) 700, 751, 851
BsgI GTGCAG 2 cut(s) 1269, 1413
Bsh1236I CGCG 1 cut(s) 1308
BshFI GGCC 4 cut(s) 51, 129, 857, 1319
BsiHKAI GWGCWC 3 cut(s) 216, 1183, 1284
BsiSI CCGG 3 cut(s) 23, 33, 48
BslFI GGGAC 4 cut(s) 105, 289, 682, 1482
BslI CCNNNNNNNGG 4 cut(s) 22, 586, 587, 879
BsmAI GTCTC 2 cut(s) 621, 1132
BsmFI GGGAC 4 cut(s) 105, 289, 682, 1482
BsnI GGCC 4 cut(s) 51, 129, 857, 1319
Bso31I GGTCTC 1 cut(s) 1132
Bsp1286I GDGCHC 4 cut(s) 216, 1183, 1284, 1304
Bsp13I TCCGGA 1 cut(s) 22
Bsp143I GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
Bsp68I TCGCGA 1 cut(s) 1308
BspACI CCGC 6 cut(s) 12, 562, 829, 1124, 1238, 1495
BspANI GGCC 4 cut(s) 51, 129, 857, 1319
BspCNI CTCAG 2 cut(s) 79, 295
BspEI TCCGGA 1 cut(s) 22
BspFNI CGCG 1 cut(s) 1308
BspLI GGNNCC 5 cut(s) 121, 856, 1108, 1214, 1489
BspMAI CTGCAG 1 cut(s) 735
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 3 cut(s) 790, 1025, 1054
BspTI CTTAAG 1 cut(s) 1138
BspTNI GGTCTC 1 cut(s) 1132
BsrBI CCGCTC 1 cut(s) 829
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 3 cut(s) 667, 1552, 1611
BssECI CCNNGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
BssMI GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
BssSI CACGAG 1 cut(s) 780
BssT1I CCWWGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
Bst2BI CACGAG 1 cut(s) 780
Bst4CI ACNGT 2 cut(s) 461, 1231
Bst6I CTCTTC 3 cut(s) 308, 402, 1062
BstAFI CTTAAG 1 cut(s) 1138
BstAPI GCANNNNNTGC 1 cut(s) 710
BstC8I GCNNGC 3 cut(s) 378, 715, 1365
BstDEI CTNAG 6 cut(s) 66, 194, 225, 303, 1380, 1409
BstEII GGTNACC 2 cut(s) 418, 991
BstF5I GGATG 7 cut(s) 154, 204, 346, 687, 1390, 1491, 1621
BstFNI CGCG 1 cut(s) 1308
BstHHI GCGC 2 cut(s) 945, 1342
BstKTI GATC 7 cut(s) 9, 798, 1033, 1049, 1137, 1521, 1602
BstMAI GTCTC 2 cut(s) 621, 1132
BstMBI GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
BstMWI GCNNNNNNNGC 7 cut(s) 86, 710, 980, 1331, 1373, 1385, 1565
BstNSI RCATGY 3 cut(s) 478, 958, 1162
BstPAI GACNNNNGTC 3 cut(s) 425, 957, 989
BstPI GGTNACC 2 cut(s) 418, 991
BstSFI CTRYAG 1 cut(s) 731
BstUI CGCG 1 cut(s) 1308
BstV1I GCAGC 6 cut(s) 208, 286, 658, 760, 1363, 1580
BstV2I GAAGAC 2 cut(s) 743, 957
BstX2I RGATCY 2 cut(s) 795, 1046
BstXI CCANNNNNNTGG 1 cut(s) 1231
BstYI RGATCY 2 cut(s) 795, 1046
BsuRI GGCC 4 cut(s) 51, 129, 857, 1319
BtgZI GCGATG 1 cut(s) 1306
BtsCI GGATG 7 cut(s) 154, 204, 346, 687, 1390, 1491, 1621
BtsI GCAGTG 2 cut(s) 1181, 1425
BtsIMutI CAGTG 3 cut(s) 370, 1181, 1425
BtuMI TCGCGA 1 cut(s) 1308
Cac8I GCNNGC 3 cut(s) 378, 715, 1365
CfoI GCGC 2 cut(s) 945, 1342
Cfr13I GGNCC 3 cut(s) 119, 855, 1212
CseI GACGC 2 cut(s) 947, 1199
Csp6I GTAC 2 cut(s) 63, 1417
CviQI GTAC 2 cut(s) 63, 1417
DdeI CTNAG 6 cut(s) 66, 194, 225, 303, 1380, 1409
DpnI GATC 7 cut(s) 8, 797, 1032, 1048, 1136, 1520, 1601
DpnII GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
DrdI GACNNNNNNGTC 1 cut(s) 1219
DseDI GACNNNNNNGTC 1 cut(s) 1219
EaeI YGGCCR 2 cut(s) 49, 1317
Eam1104I CTCTTC 3 cut(s) 308, 402, 1062
EarI CTCTTC 3 cut(s) 308, 402, 1062
Eco130I CCWWGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
Eco24I GRGCYC 1 cut(s) 1304
Eco31I GGTCTC 1 cut(s) 1132
Eco32I GATATC 1 cut(s) 492
Eco47I GGWCC 2 cut(s) 119, 1212
Eco91I GGTNACC 2 cut(s) 418, 991
EcoO109I RGGNCCY 1 cut(s) 1212
EcoO65I GGTNACC 2 cut(s) 418, 991
EcoRV GATATC 1 cut(s) 492
EcoT14I CCWWGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
EcoT38I GRGCYC 1 cut(s) 1304
ErhI CCWWGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
FalI AAGNNNNNCTT 2 cut(s) 522, 554
FaqI GGGAC 4 cut(s) 105, 289, 682, 1482
FauI CCCGC 2 cut(s) 555, 1231
FbaI TGATCA 1 cut(s) 1599
Fnu4HI GCNGC 6 cut(s) 222, 300, 672, 749, 1377, 1569
FokI GGATG 7 cut(s) 161, 211, 333, 694, 1397, 1478, 1628
FriOI GRGCYC 1 cut(s) 1304
Fsp4HI GCNGC 6 cut(s) 222, 300, 672, 749, 1377, 1569
FspBI CTAG 1 cut(s) 377
FspI TGCGCA 1 cut(s) 944
GlaI GCGC 2 cut(s) 944, 1341
GluI GCNGC 6 cut(s) 222, 300, 672, 749, 1377, 1569
GsaI CCCAGC 3 cut(s) 704, 755, 855
GsuI CTGGAG 2 cut(s) 1298, 1533
HaeIII GGCC 4 cut(s) 51, 129, 857, 1319
HapII CCGG 3 cut(s) 23, 33, 48
HgaI GACGC 2 cut(s) 947, 1199
HhaI GCGC 2 cut(s) 945, 1342
Hin6I GCGC 2 cut(s) 943, 1340
HinP1I GCGC 2 cut(s) 943, 1340
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 6 cut(s) 389, 985, 1074, 1220, 1256, 1579
HpaII CCGG 3 cut(s) 23, 33, 48
HphI GGTGA 7 cut(s) 170, 281, 430, 509, 985, 1031, 1621
Hpy166II GTNNAC 2 cut(s) 1372, 1483
Hpy188I TCNGA 3 cut(s) 69, 603, 870
Hpy188III TCNNGA 6 cut(s) 23, 254, 334, 1207, 1277, 1307
Hpy8I GTNNAC 2 cut(s) 1372, 1483
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 1606
HpyCH4III ACNGT 2 cut(s) 461, 1231
HpyCH4IV ACGT 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 710, 980, 1331, 1373, 1385, 1565
HpyF3I CTNAG 6 cut(s) 66, 194, 225, 303, 1380, 1409
HpySE526I ACGT 1 cut(s) 72
HspAI GCGC 2 cut(s) 943, 1340
Kpn2I TCCGGA 1 cut(s) 22
Ksp22I TGATCA 1 cut(s) 1599
Kzo9I GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
LmnI GCTCC 3 cut(s) 920, 980, 1279
Lsp1109I GCAGC 6 cut(s) 208, 286, 658, 760, 1363, 1580
LweI GCATC 3 cut(s) 672, 1327, 1375
MaeI CTAG 1 cut(s) 377
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 4 cut(s) 163, 418, 497, 991
MalI GATC 7 cut(s) 8, 797, 1032, 1048, 1136, 1520, 1601
MbiI CCGCTC 1 cut(s) 829
MboI GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
MboII GAAGA 6 cut(s) 325, 419, 748, 962, 1031, 1079
MfeI CAATTG 1 cut(s) 1313
MflI RGATCY 2 cut(s) 795, 1046
MhlI GDGCHC 4 cut(s) 216, 1183, 1284, 1304
MlsI TGGCCA 1 cut(s) 1319
MluCI AATT 9 cut(s) 357, 517, 864, 1313, 1328, 1394, 1501, 1545, 1587
MluNI TGGCCA 1 cut(s) 1319
MlyI GAGTC 2 cut(s) 979, 1229
MmeI TCCRAC 3 cut(s) 183, 374, 1248
Mox20I TGGCCA 1 cut(s) 1319
MroI TCCGGA 1 cut(s) 22
MroXI GAANNNNTTC 1 cut(s) 534
MscI TGGCCA 1 cut(s) 1319
MseI TTAA 6 cut(s) 105, 594, 641, 771, 900, 1139
Msp20I TGGCCA 1 cut(s) 1319
MspA1I CMGCKG 2 cut(s) 562, 751
MspCI CTTAAG 1 cut(s) 1138
MspI CCGG 3 cut(s) 23, 33, 48
MunI CAATTG 1 cut(s) 1313
MvnI CGCG 1 cut(s) 1308
MwoI GCNNNNNNNGC 7 cut(s) 86, 710, 980, 1331, 1373, 1385, 1565
NdeII GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
NheI GCTAGC 1 cut(s) 376
NlaIV GGNNCC 5 cut(s) 121, 856, 1108, 1214, 1489
NmuCI GTSAC 4 cut(s) 163, 418, 497, 991
NruI TCGCGA 1 cut(s) 1308
NsbI TGCGCA 1 cut(s) 944
NspI RCATGY 3 cut(s) 478, 958, 1162
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 4 cut(s) 389, 1074, 1256, 1579
PkrI GCNGC 6 cut(s) 223, 301, 673, 750, 1378, 1570
PleI GAGTC 2 cut(s) 979, 1228
PpsI GAGTC 2 cut(s) 979, 1228
PpuMI RGGWCCY 1 cut(s) 1212
PshAI GACNNNNGTC 3 cut(s) 425, 957, 989
PshBI ATTAAT 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 1212
PspEI GGTNACC 2 cut(s) 418, 991
PspFI CCCAGC 3 cut(s) 700, 751, 851
PspN4I GGNNCC 5 cut(s) 121, 856, 1108, 1214, 1489
PspPI GGNCC 3 cut(s) 119, 855, 1212
PspPPI RGGWCCY 1 cut(s) 1212
PstI CTGCAG 1 cut(s) 735
PsuI RGATCY 2 cut(s) 795, 1046
PvuII CAGCTG 1 cut(s) 751
RruI TCGCGA 1 cut(s) 1308
RsaI GTAC 2 cut(s) 64, 1418
RsaNI GTAC 2 cut(s) 63, 1417
SaqAI TTAA 6 cut(s) 105, 594, 641, 771, 900, 1139
SatI GCNGC 6 cut(s) 222, 300, 672, 749, 1377, 1569
Sau3AI GATC 7 cut(s) 6, 795, 1030, 1046, 1134, 1518, 1599
Sau96I GGNCC 3 cut(s) 119, 855, 1212
SchI GAGTC 2 cut(s) 979, 1229
SduI GDGCHC 4 cut(s) 216, 1183, 1284, 1304
SfaNI GCATC 3 cut(s) 672, 1327, 1375
SfcI CTRYAG 1 cut(s) 731
SinI GGWCC 2 cut(s) 119, 1212
SmlI CTYRAG 2 cut(s) 1138, 1217
SmoI CTYRAG 2 cut(s) 1138, 1217
Sse9I AATT 9 cut(s) 357, 517, 864, 1313, 1328, 1394, 1501, 1545, 1587
SsiI CCGC 6 cut(s) 12, 562, 829, 1124, 1238, 1495
SspI AATATT 2 cut(s) 100, 598
SspMI CTAG 1 cut(s) 377
StyI CCWWGG 7 cut(s) 423, 676, 969, 1042, 1296, 1437, 1624
TaaI ACNGT 2 cut(s) 461, 1231
TaiI ACGT 1 cut(s) 75
TaqI TCGA 6 cut(s) 5, 132, 210, 237, 429, 1521
TasI AATT 9 cut(s) 357, 517, 864, 1313, 1328, 1394, 1501, 1545, 1587
TfiI GAWTC 4 cut(s) 389, 1074, 1256, 1579
Tru1I TTAA 6 cut(s) 105, 594, 641, 771, 900, 1139
Tru9I TTAA 6 cut(s) 105, 594, 641, 771, 900, 1139
TscAI CASTG 3 cut(s) 370, 1188, 1432
TseFI GTSAC 4 cut(s) 163, 418, 497, 991
TseI GCWGC 6 cut(s) 221, 299, 671, 748, 1376, 1568
Tsp45I GTSAC 4 cut(s) 163, 418, 497, 991
TspGWI ACGGA 2 cut(s) 788, 1500
TspRI CASTG 3 cut(s) 370, 1188, 1432
Vha464I CTTAAG 1 cut(s) 1138
VpaK11BI GGWCC 2 cut(s) 119, 1212
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 1 cut(s) 1328
XceI RCATGY 3 cut(s) 478, 958, 1162
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.