MD06G1047900.v1.1
TCP Family

assists the folding of proteins upon ATP hydrolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010305
Physical Location & Seq
Forward (+)
6478523 .. 6485538
7016 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1047900.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGACCATGTCCTCTCAGACCCCCGATATCTTGGGCGACCGTCAGTACGGTCAGGACGTCCGCACTCAAAATGTGATGGCCTGCCAAGCTGTTGCAAACATTGTCAAATCTTCACTCGGCCCCGTTGGCCTCGACAAGATGCTTGTCGATGATATTGGTGATGTGACTATTACTAATGATGGAGCCACTATACTCAAGATGTTAGAAGTTGAGCATCCTGCTGCCAAGGTGCTTGTGGAGTTGGCTGAGCTCCAAGATCGAGAAGTTGGAGATGGGACGACGTCAGTGGTGATTGTAGCTGCAGAGTTGCTTAAGAGAGCAAATGATCTTGTAAGGAATAAGATTCACCCAACATCCATAATCAGTGGTTACAGACTTGCTATGAGGGAAGCATGCAAATATGTTGAGGAAAAATTAGCTGTGAAGGTTGACAAGCTTGGAAAAGATTCTCTAGTAAATTGTGCAAAGACAAGCATGTCCTCAAAGTTGATAGGCGGTGACAGTGACTTCTTTGCAAATATGGTTGTCGATGCAGTACAAGCTGTAAAGATGACCAATGCTCGGGGGGAAGTCAAGTACCCAATCAAGGGGATTAATGTTTTGAAAGCTCATGGAAAAAGTGCAAGAGATAGCTATCTCTTAACTGGTTTTGCACTAAATACAGGCCGTGCTGCACAAGGAATGCCACTTAGAGTTTCCCCTGCAAAGATTGCCTGTCTTGACTTTAATCTTCAGAAAACCAAAATGCAAATGGGTGTCCAAGTTTTAGTTAGTGATCCCAGGGAGCTTGAAAAAATTCGCCAAAGAGAAGCTGATATGACAAAAGAGCGTATTGAAAAACTTCTGAAAGCTGGAGCAAATGTTATTCTGACTACAAAGGGGATTGATGACATGGCACTCAAGGCAAGATTCTTTTGTGATTTGTTCCTTTCTTATTTGAAAATTTATTTTGTGGAGGCTGGGGCTATCGCTGTAAGACGTGTTCAGAAAGAAGATATGCGCCATGTTGCTAAGGCTACAGGTGCAACAATGGTTTCAACTTTCGCTGATATGGAAGGGGAGGAAACATTTGATACGTCACTTCTTGGATCTGCCGATGAAGTTGTGGAGGAGCGCATTTCTGATGATGATGTGATTTTGATAAAAGGAACAAAAAATAACAGTGCGGTGTCATTGATCCTCAGAGGTGCAAATGACTATATGCTTGATGAGATGGAAAGAGCCCTTCATGATGCTTTGTCTATTGTCAAGAGGACTCTAGAATCTAACATGGTGGTACCAGGTGGAGGTGCAGTTGAGTCCGCATTGTCTGTGTATTTGGAGAGCTTTGCTACAACATTAGGATCACGTGAGCAGTTGGCAATTGCTGAGTTTGCTGAAGCTTTGTTGATTATACCAAAGGTGCTTGCCGTCAATGCTGCCAAGGATTCTACAGATCTAGTTTCAATACTACGGGCCAACCACCACAGGGCACAAACCAAGACAACAACCAAGACTGACAAGAAGGATAAGGATTATTCTAGCATGGGCTTAGACCTATTAAAGGGAGAAGTTCGTAACAACCTTGAAGCCGGAGTCATTGAGCCTGCAATGAGTAAAGTGAAGATAATTCAGTTTGCAACTGAAGCAGCTATTACCATTCTCCGAATTGATGACATGATCAGGCTAGTCAAGGATGAAAGTCAAGAGGGTTAA

Protein Analysis

565

Amino Acids

61.51

Weight (kDa)

5.85

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 31 - 555 6.1e-155 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 475
AatII GACGTC 2 cut(s) 60, 284
Acc65I GGTACC 1 cut(s) 1276
AccB1I GGYRCC 1 cut(s) 1276
AciI CCGC 4 cut(s) 61, 495, 1166, 1302
AclWI GGATC 4 cut(s) 770, 1096, 1171, 1351
AcsI RAATTY 2 cut(s) 795, 942
AcuI CTGAAG 3 cut(s) 716, 1398, 1644
AcvI CACGTG 1 cut(s) 1349
AcyI GRCGYC 2 cut(s) 57, 281
AfaI GTAC 4 cut(s) 47, 537, 578, 1278
AfiI CCNNNNNNNGG 6 cut(s) 561, 586, 587, 1286, 1468, 1543
AflII CTTAAG 1 cut(s) 311
AflIII ACRYGT 1 cut(s) 979
AgsI TTSAA 7 cut(s) 604, 791, 836, 940, 1038, 1446, 1568
AjiI CACGTC 1 cut(s) 980
AjnI CCWGG 2 cut(s) 779, 1279
Alw21I GWGCWC 1 cut(s) 252
AlwI GGATC 4 cut(s) 770, 1096, 1171, 1351
Ama87I CYCGRG 1 cut(s) 561
AoxI GGCC 5 cut(s) 78, 118, 127, 664, 1455
ApeKI GCWGC 5 cut(s) 221, 299, 671, 1418, 1628
ApoI RAATTY 2 cut(s) 795, 942
AseI ATTAAT 1 cut(s) 594
Asp700I GAANNNNTTC 3 cut(s) 445, 795, 840
Asp718I GGTACC 1 cut(s) 1276
AspLEI GCGC 2 cut(s) 1002, 1116
AspS9I GGNCC 2 cut(s) 119, 1455
AsuHPI GGTGA 4 cut(s) 170, 301, 338, 509
AvaI CYCGRG 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 1474
BanI GGYRCC 1 cut(s) 1276
BanII GRGCYC 2 cut(s) 252, 1225
BbrPI CACGTG 1 cut(s) 1349
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 5 cut(s) 208, 286, 658, 1405, 1640
BccI CCATC 4 cut(s) 70, 173, 266, 1207
BceAI ACGGC 2 cut(s) 651, 1394
BciT130I CCWGG 2 cut(s) 781, 1281
BclI TGATCA 1 cut(s) 1659
BfaI CTAG 5 cut(s) 452, 1259, 1439, 1521, 1667
BfmI CTRYAG 3 cut(s) 300, 1017, 1431
BfrI CTTAAG 1 cut(s) 311
BglI GCCNNNNNGGC 1 cut(s) 126
BglII AGATCT 1 cut(s) 1435
BisI GCNGC 5 cut(s) 222, 300, 672, 1419, 1629
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 5 cut(s) 223, 301, 673, 1420, 1630
Bme1390I CCNGG 2 cut(s) 781, 1281
BmeT110I CYCGRG 1 cut(s) 561
BmgBI CACGTC 1 cut(s) 980
BmgT120I GGNCC 2 cut(s) 119, 1455
BmiI GGNNCC 3 cut(s) 121, 184, 1278
BmrFI CCNGG 2 cut(s) 781, 1281
BmsI GCATC 4 cut(s) 129, 223, 520, 1222
BpmI CTGGAG 1 cut(s) 873
Bpu10I CCTNAGC 1 cut(s) 1011
Bpu1102I GCTNAGC 1 cut(s) 246
BpuEI CTTGAG 2 cut(s) 179, 884
BsaAI YACGTR 1 cut(s) 1349
BsaBI GATNNNNATC 1 cut(s) 633
BsaHI GRCGYC 2 cut(s) 57, 281
BsaJI CCNNGG 4 cut(s) 225, 779, 780, 1422
BsaXI ACNNNNNCTCC 4 cut(s) 846, 876, 1278, 1308
Bsc4I CCNNNNNNNGG 6 cut(s) 561, 586, 587, 1286, 1468, 1543
Bse1I ACTGG 1 cut(s) 649
Bse3DI GCAATG 1 cut(s) 1596
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 2 cut(s) 781, 1281
BseDI CCNNGG 4 cut(s) 225, 779, 780, 1422
BseGI GGATG 3 cut(s) 214, 353, 1681
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 6 cut(s) 561, 586, 587, 1286, 1468, 1543
BseMI GCAATG 1 cut(s) 1596
BseMII CTCAG 4 cut(s) 29, 237, 1195, 1359
BseNI ACTGG 1 cut(s) 649
BseRI GAGGAG 1 cut(s) 1124
BseSI GKGCMC 1 cut(s) 1474
BseXI GCAGC 5 cut(s) 208, 286, 658, 1405, 1640
BseYI CCCAGC 1 cut(s) 959
BsgI GTGCAG 2 cut(s) 657, 1311
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 5 cut(s) 80, 120, 129, 666, 1457
BshNI GGYRCC 1 cut(s) 1276
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 1 cut(s) 1572
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 6 cut(s) 561, 586, 587, 1286, 1468, 1543
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 1 cut(s) 687
BsnI GGCC 5 cut(s) 80, 120, 129, 666, 1457
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 3 cut(s) 252, 1225, 1474
Bsp143I GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 4 cut(s) 61, 495, 1166, 1302
BspANI GGCC 5 cut(s) 80, 120, 129, 666, 1457
BspCNI CTCAG 4 cut(s) 28, 238, 1194, 1360
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 3 cut(s) 121, 184, 1278
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 4 cut(s) 770, 1096, 1171, 1351
BspT107I GGYRCC 1 cut(s) 1276
BspTI CTTAAG 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 1596
BsrI ACTGG 1 cut(s) 649
BssECI CCNNGG 4 cut(s) 225, 779, 780, 1422
BssMI GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
BssNI GRCGYC 2 cut(s) 57, 281
BssT1I CCWWGG 2 cut(s) 225, 1422
Bst2UI CCWGG 2 cut(s) 781, 1281
Bst4CI ACNGT 4 cut(s) 41, 50, 503, 1163
BstACI GRCGYC 2 cut(s) 57, 281
BstAFI CTTAAG 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 710
BstBAI YACGTR 1 cut(s) 1349
BstC8I GCNNGC 4 cut(s) 82, 394, 1407, 1587
BstDEI CTNAG 7 cut(s) 15, 246, 689, 1011, 1181, 1368, 1531
BstENI CCTNNNNNAGG 1 cut(s) 1541
BstF5I GGATG 3 cut(s) 214, 353, 1681
BstHHI GCGC 2 cut(s) 1002, 1116
BstKTI GATC 8 cut(s) 259, 328, 778, 1091, 1179, 1346, 1438, 1662
BstMBI GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 9 cut(s) 86, 126, 539, 710, 902, 1022, 1373, 1415, 1625
BstNI CCWGG 2 cut(s) 781, 1281
BstNSI RCATGY 2 cut(s) 396, 478
BstSCI CCNGG 2 cut(s) 779, 1279
BstSFI CTRYAG 3 cut(s) 300, 1017, 1431
BstSLI GKGCMC 1 cut(s) 1474
BstV1I GCAGC 5 cut(s) 208, 286, 658, 1405, 1640
BstX2I RGATCY 2 cut(s) 1088, 1435
BstYI RGATCY 2 cut(s) 1088, 1435
BsuRI GGCC 5 cut(s) 80, 120, 129, 666, 1457
BtrI CACGTC 1 cut(s) 980
BtsCI GGATG 3 cut(s) 214, 353, 1681
BtsIMutI CAGTG 4 cut(s) 291, 370, 508, 1168
Cac8I GCNNGC 4 cut(s) 82, 394, 1407, 1587
CciI TCATGA 1 cut(s) 1228
CfoI GCGC 2 cut(s) 1002, 1116
Cfr13I GGNCC 2 cut(s) 119, 1455
CsiI ACCWGGT 1 cut(s) 1279
Csp6I GTAC 4 cut(s) 46, 536, 577, 1277
CviQI GTAC 4 cut(s) 46, 536, 577, 1277
DdeI CTNAG 7 cut(s) 15, 246, 689, 1011, 1181, 1368, 1531
DpnI GATC 8 cut(s) 258, 327, 777, 1090, 1178, 1345, 1437, 1661
DpnII GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
DrdI GACNNNNNNGTC 1 cut(s) 475
DseDI GACNNNNNNGTC 1 cut(s) 475
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 225, 1422
Eco24I GRGCYC 2 cut(s) 252, 1225
Eco32I GATATC 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 3 cut(s) 716, 1398, 1644
Eco72I CACGTG 1 cut(s) 1349
Eco88I CYCGRG 1 cut(s) 561
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 1541
EcoRII CCWGG 2 cut(s) 779, 1279
EcoRV GATATC 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 225, 1422
EcoT38I GRGCYC 2 cut(s) 252, 1225
ErhI CCWWGG 2 cut(s) 225, 1422
FaqI GGGAC 1 cut(s) 289
FbaI TGATCA 1 cut(s) 1659
Fnu4HI GCNGC 5 cut(s) 222, 300, 672, 1419, 1629
FokI GGATG 3 cut(s) 201, 340, 1688
FriOI GRGCYC 2 cut(s) 252, 1225
Fsp4HI GCNGC 5 cut(s) 222, 300, 672, 1419, 1629
FspBI CTAG 5 cut(s) 452, 1259, 1439, 1521, 1667
GlaI GCGC 2 cut(s) 1001, 1115
GluI GCNGC 5 cut(s) 222, 300, 672, 1419, 1629
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 1 cut(s) 873
HaeIII GGCC 5 cut(s) 80, 120, 129, 666, 1457
HapII CCGG 1 cut(s) 1572
HhaI GCGC 2 cut(s) 1002, 1116
Hin1I GRCGYC 2 cut(s) 57, 281
Hin6I GCGC 2 cut(s) 1000, 1114
HinP1I GCGC 2 cut(s) 1000, 1114
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HindIII AAGCTT 2 cut(s) 434, 1380
HinfI GANTC 8 cut(s) 343, 446, 909, 1255, 1262, 1298, 1427, 1575
HpaII CCGG 1 cut(s) 1572
HphI GGTGA 4 cut(s) 170, 301, 338, 509
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 8 cut(s) 18, 735, 846, 870, 987, 1123, 1184, 1646
Hpy188III TCNNGA 8 cut(s) 53, 196, 260, 719, 1229, 1249, 1259, 1685
Hpy8I GTNNAC 1 cut(s) 430
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 4 cut(s) 418, 1049, 1235, 1498
HpyCH4III ACNGT 4 cut(s) 41, 50, 503, 1163
HpyCH4IV ACGT 5 cut(s) 57, 281, 979, 1076, 1348
HpyF10VI GCNNNNNNNGC 9 cut(s) 86, 126, 539, 710, 902, 1022, 1373, 1415, 1625
HpyF3I CTNAG 7 cut(s) 15, 246, 689, 1011, 1181, 1368, 1531
HpySE526I ACGT 5 cut(s) 57, 281, 979, 1076, 1348
Hsp92I GRCGYC 2 cut(s) 57, 281
HspAI GCGC 2 cut(s) 1000, 1114
KpnI GGTACC 1 cut(s) 1280
Ksp22I TGATCA 1 cut(s) 1659
Kzo9I GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
LmnI GCTCC 5 cut(s) 182, 255, 784, 854, 1111
Lsp1109I GCAGC 5 cut(s) 208, 286, 658, 1405, 1640
LweI GCATC 4 cut(s) 129, 223, 520, 1222
MabI ACCWGGT 1 cut(s) 1279
MaeI CTAG 5 cut(s) 452, 1259, 1439, 1521, 1667
MaeII ACGT 5 cut(s) 57, 281, 979, 1076, 1348
MaeIII GTNAC 6 cut(s) 163, 368, 497, 503, 1077, 1556
MalI GATC 8 cut(s) 258, 327, 777, 1090, 1178, 1345, 1437, 1661
MboI GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
MboII GAAGA 4 cut(s) 102, 722, 1004, 1615
MfeI CAATTG 1 cut(s) 1362
MflI RGATCY 2 cut(s) 1088, 1435
MhlI GDGCHC 3 cut(s) 252, 1225, 1474
MluCI AATT 7 cut(s) 413, 457, 795, 942, 1362, 1608, 1647
MlyI GAGTC 3 cut(s) 1249, 1307, 1584
MmeI TCCRAC 1 cut(s) 247
MroXI GAANNNNTTC 3 cut(s) 445, 795, 840
MseI TTAA 6 cut(s) 312, 594, 641, 726, 1541, 1693
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 1 cut(s) 1572
MspR9I CCNGG 2 cut(s) 781, 1281
MunI CAATTG 1 cut(s) 1362
Mva1269I GAATGC 1 cut(s) 687
MvaI CCWGG 2 cut(s) 781, 1281
MwoI GCNNNNNNNGC 9 cut(s) 86, 126, 539, 710, 902, 1022, 1373, 1415, 1625
NdeII GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
NlaIV GGNNCC 3 cut(s) 121, 184, 1278
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 4 cut(s) 163, 497, 503, 1077
NspI RCATGY 2 cut(s) 396, 478
PaeI GCATGC 1 cut(s) 396
PagI TCATGA 1 cut(s) 1228
PasI CCCWGGG 1 cut(s) 780
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 687
PdmI GAANNNNTTC 3 cut(s) 445, 795, 840
PfeI GAWTC 5 cut(s) 343, 446, 909, 1262, 1427
PflFI GACNNNGTC 2 cut(s) 7, 280
PkrI GCNGC 5 cut(s) 223, 301, 673, 1420, 1630
PleI GAGTC 3 cut(s) 1249, 1306, 1583
PmaCI CACGTG 1 cut(s) 1349
PmlI CACGTG 1 cut(s) 1349
PpsI GAGTC 3 cut(s) 1249, 1306, 1583
Ppu21I YACGTR 1 cut(s) 1349
PshBI ATTAAT 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 779, 1279
PspCI CACGTG 1 cut(s) 1349
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 2 cut(s) 779, 1279
PspN4I GGNNCC 3 cut(s) 121, 184, 1278
PspPI GGNCC 2 cut(s) 119, 1455
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PstI CTGCAG 1 cut(s) 304
PsuI RGATCY 2 cut(s) 1088, 1435
PsyI GACNNNGTC 2 cut(s) 7, 280
RsaI GTAC 4 cut(s) 47, 537, 578, 1278
RsaNI GTAC 4 cut(s) 46, 536, 577, 1277
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 6 cut(s) 312, 594, 641, 726, 1541, 1693
SatI GCNGC 5 cut(s) 222, 300, 672, 1419, 1629
Sau3AI GATC 8 cut(s) 256, 325, 775, 1088, 1176, 1343, 1435, 1659
Sau96I GGNCC 2 cut(s) 119, 1455
SchI GAGTC 3 cut(s) 1249, 1307, 1584
ScrFI CCNGG 2 cut(s) 781, 1281
SduI GDGCHC 3 cut(s) 252, 1225, 1474
SexAI ACCWGGT 1 cut(s) 1279
SfaNI GCATC 4 cut(s) 129, 223, 520, 1222
SfcI CTRYAG 3 cut(s) 300, 1017, 1431
SfiI GGCCNNNNNGGCC 1 cut(s) 126
SmlI CTYRAG 3 cut(s) 194, 311, 899
SmoI CTYRAG 3 cut(s) 194, 311, 899
SphI GCATGC 1 cut(s) 396
Sse9I AATT 7 cut(s) 413, 457, 795, 942, 1362, 1608, 1647
SsiI CCGC 4 cut(s) 61, 495, 1166, 1302
SspMI CTAG 5 cut(s) 452, 1259, 1439, 1521, 1667
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 779, 1279
StyI CCWWGG 2 cut(s) 225, 1422
TaaI ACNGT 4 cut(s) 41, 50, 503, 1163
TaiI ACGT 5 cut(s) 60, 284, 982, 1079, 1351
TaqI TCGA 4 cut(s) 132, 147, 259, 528
TasI AATT 7 cut(s) 413, 457, 795, 942, 1362, 1608, 1647
TatI WGTACW 1 cut(s) 535
TfiI GAWTC 5 cut(s) 343, 446, 909, 1262, 1427
Tru1I TTAA 6 cut(s) 312, 594, 641, 726, 1541, 1693
Tru9I TTAA 6 cut(s) 312, 594, 641, 726, 1541, 1693
TscAI CASTG 4 cut(s) 291, 370, 508, 1168
TseFI GTSAC 4 cut(s) 163, 497, 503, 1077
TseI GCWGC 5 cut(s) 221, 299, 671, 1418, 1628
Tsp45I GTSAC 4 cut(s) 163, 497, 503, 1077
TspDTI ATGAA 3 cut(s) 1113, 1217, 1692
TspRI CASTG 4 cut(s) 291, 370, 508, 1168
Tth111I GACNNNGTC 2 cut(s) 7, 280
Vha464I CTTAAG 1 cut(s) 311
VspI ATTAAT 1 cut(s) 594
XagI CCTNNNNNAGG 1 cut(s) 1541
XapI RAATTY 2 cut(s) 795, 942
XbaI TCTAGA 1 cut(s) 1258
XceI RCATGY 2 cut(s) 396, 478
XcmI CCANNNNNNNNNTGG 2 cut(s) 232, 748
XmnI GAANNNNTTC 3 cut(s) 445, 795, 840
XspI CTAG 5 cut(s) 452, 1259, 1439, 1521, 1667
ZraI GACGTC 2 cut(s) 58, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.