AT3G22630

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010269
Physical Location & Seq
Reverse (-)
8009540 .. 8010862
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22630.1

Sequence Viewer

Length: 615 bp
ATGGAGTGTGTATTCGGTCTTGTTGGGAATGGATTCGCCATCGTTGCTGCTGATACATCGGCGGTTCATAGTATCCTTCTTCACAAAAACAACGAAGACAAGATCATGGTACTTGACTCACACAAGCTTGTTGCTGCTAGTGGCGAGCCTGGTGACCGGGTTCAGTTTACGGAGTACGTTCAGAAGAATGTGTCCTTGTACAAATTCAGAAATGGAATCCCGTTGACTACCGCCGCTGCTGCCAATTTCACTCGCGGAGAGCTCGCTACTGCTTTGAGGAAGAATCCGTATTCGGTGAATATCCTGATGGCTGGCTATGACGATGAGTCTGGTGCATCACTTTACTACATCGACTACATTGCAACTCTTCACAAAGTCGACAAGGGAGCTTTTGGTTACGGCTCCTACTTCTCCCTCTCCACGATGGACAGGCATTACCGCAGTGACATGTCTGTAGAAGAAGCCATTGAACTAGTTGACAAATGCATACTTGAGATCCGGTCAAGACTCGTTGTTGCACCACCAAACTTTGTGATCAAGATTGTTGACAAAGATGGAGCTCGTGATTACGCTTGGCGTCAGTCCGTAAAGGATGTCACAACTGCTGTTGTCTGA

Protein Analysis

204

Amino Acids

22.54

Weight (kDa)

5.95

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 3 - 182 3.5e-42 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22630 AT4G14800 AT4G14800
fragaria_vesca FvH4_4g15960 FvH4_7g08790
malus_domestica MD13G1196600.v1.1 MD16G1197000.v1.1
prunus_persica Prupe.1G017400_v2.0.a1 Prupe.1G017400_v2.0.a1
pyrus_communis pycom01g08470 pycom16g16580
rosa_chinensis RchiOBHm_Chr4g0415561
rosa_laevigata RLG00000008063
rosa_multiflora Rmu_sc0004599.1_g000004
rosa_roxburghii Rroxscaffold_5G00359380
rosa_rugosa Rorug04G0134700
rosa_samantha Rh4AG196100 Rh4BG194800 Rh4CG206800 Rh4DG193600
rosa_wichuraiana Rw4G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 378
AccII CGCG 1 cut(s) 255
AciI CCGC 5 cut(s) 62, 231, 234, 255, 439
AclWI GGATC 1 cut(s) 490
AcsI RAATTY 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 3 cut(s) 111, 176, 200
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 1 cut(s) 470
AhdI GACNNNNNGTC 1 cut(s) 325
AhlI ACTAGT 1 cut(s) 472
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 4 cut(s) 127, 262, 389, 560
AluI AGCT 4 cut(s) 127, 262, 389, 560
Alw21I GWGCWC 2 cut(s) 264, 562
AlwI GGATC 1 cut(s) 490
ApeKI GCWGC 4 cut(s) 47, 134, 236, 239
ApoI RAATTY 1 cut(s) 203
Asp700I GAANNNNTTC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 2 cut(s) 164, 307
BanII GRGCYC 2 cut(s) 264, 562
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 264, 562
BbvI GCAGC 4 cut(s) 34, 121, 223, 226
BccI CCATC 4 cut(s) 47, 301, 418, 548
BceAI ACGGC 1 cut(s) 415
BcgI CGANNNNNNTGC 2 cut(s) 341, 375
BciT130I CCWGG 1 cut(s) 150
BciVI GTATCC 1 cut(s) 83
BclI TGATCA 1 cut(s) 534
BcnI CCSGG 1 cut(s) 158
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 2 cut(s) 138, 473
BfmI CTRYAG 1 cut(s) 453
BfuI GTATCC 1 cut(s) 83
BisI GCNGC 5 cut(s) 48, 135, 234, 237, 240
BlsI GCNGC 5 cut(s) 49, 136, 235, 238, 241
Bme1390I CCNGG 2 cut(s) 150, 158
BmeRI GACNNNNNGTC 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 2 cut(s) 150, 158
BmsI GCATC 1 cut(s) 344
BpiI GAAGAC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 512
BpuMI CCSGG 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 577
BsaWI WCCGGW 1 cut(s) 498
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 150
BseGI GGATG 1 cut(s) 598
BseMI GCAATG 1 cut(s) 357
BseXI GCAGC 4 cut(s) 34, 121, 223, 226
Bsh1236I CGCG 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 264, 562
BsiSI CCGG 2 cut(s) 157, 499
Bsp1286I GDGCHC 2 cut(s) 264, 562
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 3 cut(s) 102, 495, 534
BspACI CCGC 5 cut(s) 62, 231, 234, 255, 439
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 357
BsrGI TGTACA 1 cut(s) 198
BssMI GATC 3 cut(s) 102, 495, 534
BssNI GRCGYC 1 cut(s) 577
BssSI CACGAG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 150
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 577
BstAUI TGTACA 1 cut(s) 198
BstC8I GCNNGC 3 cut(s) 146, 264, 313
BstEII GGTNACC 1 cut(s) 152
BstF5I GGATG 1 cut(s) 598
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 3 cut(s) 105, 498, 537
BstMBI GATC 3 cut(s) 102, 495, 534
BstMWI GCNNNNNNNGC 2 cut(s) 44, 239
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 1 cut(s) 451
BstPI GGTNACC 1 cut(s) 152
BstSCI CCNGG 2 cut(s) 148, 156
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 4 cut(s) 34, 121, 223, 226
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 495
BstYI RGATCY 1 cut(s) 495
BsuI GTATCC 1 cut(s) 83
BtsCI GGATG 1 cut(s) 598
BtsI GCAGTG 1 cut(s) 448
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 3 cut(s) 146, 264, 313
CseI GACGC 1 cut(s) 566
Csp6I GTAC 3 cut(s) 110, 175, 199
CviAII CATG 2 cut(s) 106, 448
CviJI RGCY 9 cut(s) 127, 148, 262, 311, 315, 389, 402, 464, 560
CviKI_1 RGCY 9 cut(s) 127, 148, 262, 311, 315, 389, 402, 464, 560
CviQI GTAC 3 cut(s) 110, 175, 199
DpnI GATC 3 cut(s) 104, 497, 536
DpnII GATC 3 cut(s) 102, 495, 534
DriI GACNNNNNGTC 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 372
Eam1105I GACNNNNNGTC 1 cut(s) 325
EarI CTCTTC 1 cut(s) 372
Ecl136II GAGCTC 2 cut(s) 262, 560
Eco24I GRGCYC 2 cut(s) 264, 562
Eco53kI GAGCTC 2 cut(s) 262, 560
Eco91I GGTNACC 1 cut(s) 152
EcoICRI GAGCTC 2 cut(s) 262, 560
EcoO65I GGTNACC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 2 cut(s) 264, 562
FaeI CATG 2 cut(s) 109, 451
FaiI YATR 5 cut(s) 69, 107, 318, 449, 488
FatI CATG 2 cut(s) 105, 447
FbaI TGATCA 1 cut(s) 534
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 5 cut(s) 48, 135, 234, 237, 240
FokI GGATG 1 cut(s) 605
FriOI GRGCYC 2 cut(s) 264, 562
Fsp4HI GCNGC 5 cut(s) 48, 135, 234, 237, 240
FspBI CTAG 2 cut(s) 138, 473
GluI GCNGC 5 cut(s) 48, 135, 234, 237, 240
HapII CCGG 2 cut(s) 157, 499
HgaI GACGC 1 cut(s) 566
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 2 cut(s) 109, 451
HincII GTYRAC 4 cut(s) 225, 379, 478, 547
HindII GTYRAC 4 cut(s) 225, 379, 478, 547
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 6 cut(s) 33, 116, 216, 283, 326, 507
HpaII CCGG 2 cut(s) 157, 499
HphI GGTGA 2 cut(s) 164, 307
Hpy166II GTNNAC 5 cut(s) 168, 225, 379, 478, 547
Hpy188I TCNGA 3 cut(s) 183, 209, 614
Hpy188III TCNNGA 4 cut(s) 304, 504, 538, 563
Hpy8I GTNNAC 5 cut(s) 168, 225, 379, 478, 547
HpyAV CCTTC 1 cut(s) 86
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 4 cut(s) 335, 362, 486, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 239
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 2 cut(s) 109, 451
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 3 cut(s) 102, 495, 534
LmnI GCTCC 3 cut(s) 386, 407, 557
LpnPI CCDG 8 cut(s) 135, 162, 170, 297, 315, 317, 415, 512
Lsp1109I GCAGC 4 cut(s) 34, 121, 223, 226
LweI GCATC 1 cut(s) 344
MaeI CTAG 2 cut(s) 138, 473
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 4 cut(s) 152, 395, 443, 595
MalI GATC 3 cut(s) 104, 497, 536
MboI GATC 3 cut(s) 102, 495, 534
MboII GAAGA 6 cut(s) 71, 107, 196, 292, 359, 470
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 2 cut(s) 264, 562
MluCI AATT 2 cut(s) 203, 244
MlyI GAGTC 3 cut(s) 110, 335, 501
MnlI CCTC 2 cut(s) 270, 425
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 2 cut(s) 157, 499
MspR9I CCNGG 2 cut(s) 150, 158
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 2 cut(s) 44, 239
NciI CCSGG 1 cut(s) 158
NdeII GATC 3 cut(s) 102, 495, 534
NlaIII CATG 2 cut(s) 109, 451
NlaIV GGNNCC 1 cut(s) 403
NmuCI GTSAC 3 cut(s) 152, 443, 595
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 1 cut(s) 451
PciI ACATGT 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 3 cut(s) 33, 216, 283
PkrI GCNGC 5 cut(s) 49, 136, 235, 238, 241
PleI GAGTC 3 cut(s) 110, 334, 501
PpsI GAGTC 3 cut(s) 110, 334, 501
PscI ACATGT 1 cut(s) 447
Psp124BI GAGCTC 2 cut(s) 264, 562
Psp6I CCWGG 1 cut(s) 148
PspEI GGTNACC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 403
PsuI RGATCY 1 cut(s) 495
RsaI GTAC 3 cut(s) 111, 176, 200
RsaNI GTAC 3 cut(s) 110, 175, 199
SacI GAGCTC 2 cut(s) 264, 562
SalI GTCGAC 1 cut(s) 377
SatI GCNGC 5 cut(s) 48, 135, 234, 237, 240
Sau3AI GATC 3 cut(s) 102, 495, 534
SchI GAGTC 3 cut(s) 110, 335, 501
ScrFI CCNGG 2 cut(s) 150, 158
SduI GDGCHC 2 cut(s) 264, 562
SetI ASST 5 cut(s) 129, 180, 264, 391, 562
SfaNI GCATC 1 cut(s) 344
SfcI CTRYAG 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
SpeI ACTAGT 1 cut(s) 472
Sse9I AATT 2 cut(s) 203, 244
SsiI CCGC 5 cut(s) 62, 231, 234, 255, 439
SspMI CTAG 2 cut(s) 138, 473
SstI GAGCTC 2 cut(s) 264, 562
StyD4I CCNGG 2 cut(s) 148, 156
TaiI ACGT 1 cut(s) 180
TaqI TCGA 2 cut(s) 351, 378
TaqII GACCGA 1 cut(s) 5
TasI AATT 2 cut(s) 203, 244
TatI WGTACW 1 cut(s) 198
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 3 cut(s) 33, 216, 283
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 3 cut(s) 152, 443, 595
TseI GCWGC 4 cut(s) 47, 134, 236, 239
Tsp45I GTSAC 3 cut(s) 152, 443, 595
TspDTI ATGAA 1 cut(s) 56
TspGWI ACGGA 3 cut(s) 185, 276, 574
TspRI CASTG 1 cut(s) 448
XapI RAATTY 1 cut(s) 203
XceI RCATGY 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 378
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 2 cut(s) 138, 473
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.