pycom16g16580

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010269
Physical Location & Seq
Reverse (-)
12457550 .. 12458127
578 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16580.1

Sequence Viewer

Length: 459 bp
ATGAGTGAACTTGTTCCTCAAGTTGCCTCCACTGCTATTACCTCCAAATTTTTTAACCCAAGAGCAGAATACAGTGTACCCCAGCTCCAAAGAAATCAGAAAACCCAGAGAGAGAGATCGAAACTCGACAAGCCAGCAGATCAACAAGCAATGGAGTCCATCTTCGGTTTGGTAGGCGATGGCTTCGCTGTCGTGGCAGCGGACACTTCAGCCGTGCACAGCATACTGGTCCACAAGTCCAACGAAGACAAGATCATGGTGCTCGATTCCCACAAGCTCGTCGCTGCTAGCGGCGAGCCCGGCGACAGGGTTCAGTTCACCGAATACATACAGAAGAACGTGGCATTGTACCAGTTCCGGAACGGGATTCCGTTGACCACCGCTGCTGCTGCCAATTTCACCCGCGGTGAGCTCGCCACTGCCTTGCGAAAGGTATGGGCTTTTTCTTGGATACGTTAA

Protein Analysis

153

Amino Acids

16.84

Weight (kDa)

9.3

Isoelectric Point (pI)

24.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 53 - 140 2.2e-16 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22630 AT4G14800 AT4G14800
fragaria_vesca FvH4_4g15960 FvH4_7g08790
malus_domestica MD13G1196600.v1.1 MD16G1197000.v1.1
prunus_persica Prupe.1G017400_v2.0.a1 Prupe.1G017400_v2.0.a1
pyrus_communis pycom01g08470 pycom16g16580
rosa_chinensis RchiOBHm_Chr4g0415561
rosa_laevigata RLG00000008063
rosa_multiflora Rmu_sc0004599.1_g000004
rosa_roxburghii Rroxscaffold_5G00359380
rosa_rugosa Rorug04G0134700
rosa_samantha Rh4AG196100 Rh4BG194800 Rh4CG206800 Rh4DG193600
rosa_wichuraiana Rw4G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 405
AccIII TCCGGA 1 cut(s) 357
AciI CCGC 5 cut(s) 200, 291, 381, 403, 405
AcsI RAATTY 1 cut(s) 47
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 2 cut(s) 78, 350
AfiI CCNNNNNNNGG 1 cut(s) 306
AluBI AGCT 3 cut(s) 85, 277, 412
AluI AGCT 3 cut(s) 85, 277, 412
Alw21I GWGCWC 3 cut(s) 219, 264, 414
Alw44I GTGCAC 1 cut(s) 215
Aor13HI TCCGGA 1 cut(s) 357
ApaLI GTGCAC 1 cut(s) 215
ApeKI GCWGC 5 cut(s) 197, 284, 383, 386, 389
ApoI RAATTY 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 229
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 3 cut(s) 310, 391, 419
AsuNHI GCTAGC 1 cut(s) 287
AvaII GGWCC 1 cut(s) 229
BaeGI GKGCMC 1 cut(s) 219
BanII GRGCYC 2 cut(s) 300, 414
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 3 cut(s) 219, 264, 414
BbvI GCAGC 5 cut(s) 209, 271, 370, 373, 376
BccI CCATC 2 cut(s) 167, 173
BceAI ACGGC 1 cut(s) 197
BciVI GTATCC 1 cut(s) 444
BcnI CCSGG 1 cut(s) 300
BfaI CTAG 1 cut(s) 288
BfuI GTATCC 1 cut(s) 444
BisI GCNGC 6 cut(s) 198, 285, 292, 384, 387, 390
BlsI GCNGC 6 cut(s) 199, 286, 293, 385, 388, 391
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 1 cut(s) 229
BmrFI CCNGG 1 cut(s) 300
BmtI GCTAGC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 403
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse1I ACTGG 2 cut(s) 231, 352
Bse3DI GCAATG 1 cut(s) 156
BseAI TCCGGA 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 403
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMI GCAATG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 231, 352
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 5 cut(s) 209, 271, 370, 373, 376
BseYI CCCAGC 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 405
BsiHKAI GWGCWC 3 cut(s) 219, 264, 414
BsiSI CCGG 2 cut(s) 300, 358
BslI CCNNNNNNNGG 1 cut(s) 306
Bsp1286I GDGCHC 4 cut(s) 219, 264, 300, 414
Bsp13I TCCGGA 1 cut(s) 357
Bsp143I GATC 3 cut(s) 116, 139, 252
BspACI CCGC 5 cut(s) 200, 291, 381, 403, 405
BspEI TCCGGA 1 cut(s) 357
BspFNI CGCG 1 cut(s) 405
BspOI GCTAGC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 156
BsrI ACTGG 2 cut(s) 231, 352
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 3 cut(s) 116, 139, 252
Bst4CI ACNGT 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 135, 289, 296, 414
BstDSI CCRYGG 1 cut(s) 403
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 3 cut(s) 119, 142, 255
BstMBI GATC 3 cut(s) 116, 139, 252
BstMWI GCNNNNNNNGC 4 cut(s) 32, 194, 300, 389
BstSCI CCNGG 1 cut(s) 298
BstSLI GKGCMC 1 cut(s) 219
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 5 cut(s) 209, 271, 370, 373, 376
BstV2I GAAGAC 1 cut(s) 252
BsuI GTATCC 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 403
BtgZI GCGATG 1 cut(s) 192
BtsI GCAGTG 2 cut(s) 30, 417
BtsIMutI CAGTG 3 cut(s) 30, 79, 417
Cac8I GCNNGC 4 cut(s) 135, 289, 296, 414
Cfr13I GGNCC 1 cut(s) 229
Cfr42I CCGCGG 1 cut(s) 406
Csp6I GTAC 2 cut(s) 77, 349
CviAII CATG 1 cut(s) 256
CviJI RGCY 8 cut(s) 85, 133, 183, 212, 277, 298, 412, 440
CviKI_1 RGCY 8 cut(s) 85, 133, 183, 212, 277, 298, 412, 440
CviQI GTAC 2 cut(s) 77, 349
DpnI GATC 3 cut(s) 118, 141, 254
DpnII GATC 3 cut(s) 116, 139, 252
Ecl136II GAGCTC 1 cut(s) 412
Eco24I GRGCYC 2 cut(s) 300, 414
Eco47I GGWCC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 412
EcoT38I GRGCYC 2 cut(s) 300, 414
FaeI CATG 1 cut(s) 259
FaiI YATR 4 cut(s) 224, 257, 329, 436
FatI CATG 1 cut(s) 255
FauI CCCGC 1 cut(s) 410
Fnu4HI GCNGC 6 cut(s) 198, 285, 292, 384, 387, 390
FriOI GRGCYC 2 cut(s) 300, 414
Fsp4HI GCNGC 6 cut(s) 198, 285, 292, 384, 387, 390
FspBI CTAG 1 cut(s) 288
GluI GCNGC 6 cut(s) 198, 285, 292, 384, 387, 390
GsaI CCCAGC 1 cut(s) 85
HapII CCGG 2 cut(s) 300, 358
Hin1II CATG 1 cut(s) 259
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 3 cut(s) 155, 266, 367
HpaII CCGG 2 cut(s) 300, 358
HphI GGTGA 3 cut(s) 310, 391, 419
Hpy166II GTNNAC 6 cut(s) 8, 77, 217, 232, 318, 375
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 1 cut(s) 358
Hpy8I GTNNAC 6 cut(s) 8, 77, 217, 232, 318, 375
Hpy99I CGWCG 1 cut(s) 284
HpyCH4III ACNGT 1 cut(s) 74
HpyCH4IV ACGT 2 cut(s) 339, 454
HpyCH4V TGCA 1 cut(s) 217
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 194, 300, 389
HpySE526I ACGT 2 cut(s) 339, 454
Hsp92II CATG 1 cut(s) 259
Kpn2I TCCGGA 1 cut(s) 357
KspI CCGCGG 1 cut(s) 406
Kzo9I GATC 3 cut(s) 116, 139, 252
LmnI GCTCC 1 cut(s) 90
LpnPI CCDG 8 cut(s) 95, 119, 147, 212, 292, 313, 365, 371
Lsp1109I GCAGC 5 cut(s) 209, 271, 370, 373, 376
MaeI CTAG 1 cut(s) 288
MaeII ACGT 2 cut(s) 339, 454
MalI GATC 3 cut(s) 118, 141, 254
MboI GATC 3 cut(s) 116, 139, 252
MboII GAAGA 3 cut(s) 154, 257, 346
MhlI GDGCHC 4 cut(s) 219, 264, 300, 414
MluCI AATT 2 cut(s) 47, 394
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 3 cut(s) 27, 37, 52
MroI TCCGGA 1 cut(s) 357
MroXI GAANNNNTTC 1 cut(s) 12
MseI TTAA 2 cut(s) 54, 457
MspA1I CMGCKG 3 cut(s) 200, 383, 405
MspI CCGG 2 cut(s) 300, 358
MspR9I CCNGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 4 cut(s) 32, 194, 300, 389
NciI CCSGG 1 cut(s) 300
NdeII GATC 3 cut(s) 116, 139, 252
NheI GCTAGC 1 cut(s) 287
NlaIII CATG 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 266, 367
PkrI GCNGC 6 cut(s) 199, 286, 293, 385, 388, 391
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 414
PspFI CCCAGC 1 cut(s) 81
PspPI GGNCC 1 cut(s) 229
RsaI GTAC 2 cut(s) 78, 350
RsaNI GTAC 2 cut(s) 77, 349
SacI GAGCTC 1 cut(s) 414
SacII CCGCGG 1 cut(s) 406
SaqAI TTAA 2 cut(s) 54, 457
SatI GCNGC 6 cut(s) 198, 285, 292, 384, 387, 390
Sau3AI GATC 3 cut(s) 116, 139, 252
Sau96I GGNCC 1 cut(s) 229
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 4 cut(s) 219, 264, 300, 414
SetI ASST 7 cut(s) 44, 87, 279, 342, 414, 435, 457
Sfr303I CCGCGG 1 cut(s) 406
SgrBI CCGCGG 1 cut(s) 406
SinI GGWCC 1 cut(s) 229
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 2 cut(s) 47, 394
SsiI CCGC 5 cut(s) 200, 291, 381, 403, 405
SspMI CTAG 1 cut(s) 288
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 74
TaiI ACGT 2 cut(s) 342, 457
TaqI TCGA 3 cut(s) 119, 126, 264
TasI AATT 2 cut(s) 47, 394
TauI GCSGC 1 cut(s) 294
TfiI GAWTC 2 cut(s) 266, 367
Tru1I TTAA 2 cut(s) 54, 457
Tru9I TTAA 2 cut(s) 54, 457
TscAI CASTG 3 cut(s) 37, 79, 424
TseI GCWGC 5 cut(s) 197, 284, 383, 386, 389
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 3 cut(s) 37, 79, 424
VneI GTGCAC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 1 cut(s) 47
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.