AT4G14800

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010269
Physical Location & Seq
Forward (+)
8500193 .. 8502589
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14800.1

Sequence Viewer

Length: 600 bp
ATGGAGTGTGTATTTGGTCTAGTTGGGAATGGATTCGCTATCGTGGCGGCGGATACATCAGCAGTTCACAGTATTCTTCTTCACAAGAACAAAGAGGACAAGATCATGACTCTTGACTCTCACAAGCTCGTCGCCGCTAGTGGTGAGCCTGGTGACCGGGTTCAGTTTACGGAGTATGTTCAGAAGAATGTGTCACTGTATCAGTTTCGTAATGGGATTCCTTTGAGTACTGCTGCTGCTGCTAACTTCACTCGTGGAGAACTTGCCACTGCTTTGAGAAAGAATCCATATTCGGTGAACATCCTGATGGCTGGCTATGACAAAGAAGCCGGCGCATCTCTATACTACATTGACTACATTGCAACCCTCCACAAGGTTGATAAGGGAGCATTTGGATACGGCTCATACTTCTCTCTCTCTACAATGGACAGACACTACCGCAGCGACATGTCTGTTGAAGAAGCCATTGAATTGGTGGACAAATGCATACTCGAGATCAGGTCAAGGCTGGTCATTGCACCACCAAACTTTGTTATCAAGATTGTTGACAAGGACGGAGCCCGTGAGTACGGATGGCGTATCAGCACTGCTGATGCCTAA

Protein Analysis

199

Amino Acids

21.98

Weight (kDa)

6.21

Isoelectric Point (pI)

26.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 3 - 182 5.8e-42 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22630 AT4G14800 AT4G14800
fragaria_vesca FvH4_4g15960 FvH4_7g08790
malus_domestica MD13G1196600.v1.1 MD16G1197000.v1.1
prunus_persica Prupe.1G017400_v2.0.a1 Prupe.1G017400_v2.0.a1
pyrus_communis pycom01g08470 pycom16g16580
rosa_chinensis RchiOBHm_Chr4g0415561
rosa_laevigata RLG00000008063
rosa_multiflora Rmu_sc0004599.1_g000004
rosa_roxburghii Rroxscaffold_5G00359380
rosa_rugosa Rorug04G0134700
rosa_samantha Rh4AG196100 Rh4BG194800 Rh4CG206800 Rh4DG193600
rosa_wichuraiana Rw4G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 47, 50, 135, 439
AfaI GTAC 2 cut(s) 229, 569
AfiI CCNNNNNNNGG 1 cut(s) 373
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 2 cut(s) 458, 470
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 491
ApeKI GCWGC 4 cut(s) 233, 236, 239, 441
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 155, 164, 307
AvaI CYCGRG 1 cut(s) 491
BanII GRGCYC 1 cut(s) 562
BauI CACGAG 1 cut(s) 252
BbvI GCAGC 4 cut(s) 220, 223, 226, 453
BccI CCATC 2 cut(s) 301, 567
BceAI ACGGC 1 cut(s) 415
BciT130I CCWGG 1 cut(s) 150
BciVI GTATCC 2 cut(s) 46, 389
BcnI CCSGG 1 cut(s) 158
BfaI CTAG 2 cut(s) 20, 138
BfuI GTATCC 2 cut(s) 46, 389
BisI GCNGC 6 cut(s) 48, 135, 234, 237, 240, 442
BlsI GCNGC 6 cut(s) 49, 136, 235, 238, 241, 443
BmcAI AGTACT 1 cut(s) 229
Bme1390I CCNGG 2 cut(s) 150, 158
BmeT110I CYCGRG 1 cut(s) 491
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 2 cut(s) 150, 158
BmsI GCATC 2 cut(s) 344, 583
BpuMI CCSGG 1 cut(s) 158
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse118I RCCGGY 1 cut(s) 329
Bse3DI GCAATG 2 cut(s) 357, 513
BseBI CCWGG 1 cut(s) 150
BseGI GGATG 2 cut(s) 300, 578
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 2 cut(s) 357, 513
BseXI GCAGC 4 cut(s) 220, 223, 226, 453
BsiHKCI CYCGRG 1 cut(s) 491
BsiSI CCGG 2 cut(s) 157, 330
BslI CCNNNNNNNGG 1 cut(s) 373
BsoBI CYCGRG 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 2 cut(s) 102, 495
BspACI CCGC 4 cut(s) 47, 50, 135, 439
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 559
BsrDI GCAATG 2 cut(s) 357, 513
BsrFI RCCGGY 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 329
BssMI GATC 2 cut(s) 102, 495
BssSI CACGAG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 71, 198
BstC8I GCNNGC 2 cut(s) 313, 331
BstEII GGTNACC 1 cut(s) 152
BstENI CCTNNNNNAGG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 300, 578
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 2 cut(s) 105, 498
BstMBI GATC 2 cut(s) 102, 495
BstMWI GCNNNNNNNGC 2 cut(s) 44, 239
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 1 cut(s) 451
BstPI GGTNACC 1 cut(s) 152
BstSCI CCNGG 2 cut(s) 148, 156
BstV1I GCAGC 4 cut(s) 220, 223, 226, 453
BstXI CCANNNNNNTGG 1 cut(s) 472
BsuI GTATCC 2 cut(s) 46, 389
BtsCI GGATG 2 cut(s) 300, 578
BtsI GCAGTG 2 cut(s) 267, 585
BtsIMutI CAGTG 3 cut(s) 194, 267, 585
Cac8I GCNNGC 2 cut(s) 313, 331
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 335
Cfr10I RCCGGY 1 cut(s) 329
Csp6I GTAC 2 cut(s) 228, 568
CviAII CATG 2 cut(s) 106, 448
CviJI RGCY 9 cut(s) 127, 148, 311, 315, 329, 402, 464, 508, 560
CviKI_1 RGCY 9 cut(s) 127, 148, 311, 315, 329, 402, 464, 508, 560
CviQI GTAC 2 cut(s) 228, 568
DpnI GATC 2 cut(s) 104, 497
DpnII GATC 2 cut(s) 102, 495
EciI GGCGGA 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 562
Eco88I CYCGRG 1 cut(s) 491
Eco91I GGTNACC 1 cut(s) 152
EcoNI CCTNNNNNAGG 1 cut(s) 371
EcoO65I GGTNACC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 562
FaeI CATG 2 cut(s) 109, 451
FaiI YATR 8 cut(s) 107, 177, 289, 318, 343, 406, 449, 488
FatI CATG 2 cut(s) 105, 447
Fnu4HI GCNGC 6 cut(s) 48, 135, 234, 237, 240, 442
FokI GGATG 2 cut(s) 287, 585
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 6 cut(s) 48, 135, 234, 237, 240, 442
FspBI CTAG 2 cut(s) 20, 138
GlaI GCGC 1 cut(s) 334
GluI GCNGC 6 cut(s) 48, 135, 234, 237, 240, 442
HapII CCGG 2 cut(s) 157, 330
HhaI GCGC 1 cut(s) 335
Hin1II CATG 2 cut(s) 109, 451
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 5 cut(s) 33, 109, 116, 217, 283
HpaII CCGG 2 cut(s) 157, 330
HphI GGTGA 3 cut(s) 155, 164, 307
Hpy166II GTNNAC 5 cut(s) 67, 168, 298, 478, 547
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 5 cut(s) 106, 113, 304, 493, 538
Hpy8I GTNNAC 5 cut(s) 67, 168, 298, 478, 547
Hpy99I CGWCG 1 cut(s) 134
HpyCH4III ACNGT 2 cut(s) 71, 198
HpyCH4V TGCA 3 cut(s) 362, 486, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 239
Hsp92II CATG 2 cut(s) 109, 451
HspAI GCGC 1 cut(s) 333
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 2 cut(s) 102, 495
LmnI GCTCC 2 cut(s) 386, 557
LpnPI CCDG 8 cut(s) 135, 162, 170, 297, 317, 343, 484, 494
Lsp1109I GCAGC 4 cut(s) 220, 223, 226, 453
LweI GCATC 2 cut(s) 344, 583
MaeI CTAG 2 cut(s) 20, 138
MaeIII GTNAC 2 cut(s) 152, 192
MalI GATC 2 cut(s) 104, 497
MboI GATC 2 cut(s) 102, 495
MboII GAAGA 4 cut(s) 68, 71, 196, 470
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 1 cut(s) 470
MlyI GAGTC 2 cut(s) 103, 110
MnlI CCTC 2 cut(s) 88, 377
Mph1103I ATGCAT 1 cut(s) 488
MroNI GCCGGC 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 2 cut(s) 157, 330
MspR9I CCNGG 2 cut(s) 150, 158
MvaI CCWGG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 44, 239
NaeI GCCGGC 1 cut(s) 331
NciI CCSGG 1 cut(s) 158
NdeII GATC 2 cut(s) 102, 495
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 2 cut(s) 109, 451
NlaIV GGNNCC 1 cut(s) 559
NmuCI GTSAC 2 cut(s) 152, 192
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 491
PagI TCATGA 1 cut(s) 105
PciI ACATGT 1 cut(s) 447
PdiI GCCGGC 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 3 cut(s) 33, 217, 283
PkrI GCNGC 6 cut(s) 49, 136, 235, 238, 241, 443
PleI GAGTC 2 cut(s) 103, 110
PpsI GAGTC 2 cut(s) 103, 110
PscI ACATGT 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 148
PspEI GGTNACC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 1 cut(s) 559
RsaI GTAC 2 cut(s) 229, 569
RsaNI GTAC 2 cut(s) 228, 568
RseI CAYNNNNRTG 1 cut(s) 305
SatI GCNGC 6 cut(s) 48, 135, 234, 237, 240, 442
Sau3AI GATC 2 cut(s) 102, 495
ScaI AGTACT 1 cut(s) 229
SchI GAGTC 2 cut(s) 103, 110
ScrFI CCNGG 2 cut(s) 150, 158
SduI GDGCHC 1 cut(s) 562
SetI ASST 3 cut(s) 129, 378, 503
SfaNI GCATC 2 cut(s) 344, 583
Sfr274I CTCGAG 1 cut(s) 491
SlaI CTCGAG 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 1 cut(s) 470
SsiI CCGC 4 cut(s) 47, 50, 135, 439
SspMI CTAG 2 cut(s) 20, 138
StyD4I CCNGG 2 cut(s) 148, 156
TaaI ACNGT 2 cut(s) 71, 198
TaqI TCGA 1 cut(s) 492
TasI AATT 1 cut(s) 470
TatI WGTACW 1 cut(s) 227
TauI GCSGC 2 cut(s) 50, 137
TfiI GAWTC 3 cut(s) 33, 217, 283
TscAI CASTG 3 cut(s) 201, 274, 592
TseFI GTSAC 2 cut(s) 152, 192
TseI GCWGC 4 cut(s) 233, 236, 239, 441
Tsp45I GTSAC 2 cut(s) 152, 192
TspGWI ACGGA 3 cut(s) 185, 570, 585
TspRI CASTG 3 cut(s) 201, 274, 592
XagI CCTNNNNNAGG 1 cut(s) 371
XceI RCATGY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XhoI CTCGAG 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 2 cut(s) 20, 138
ZrmI AGTACT 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.