MD13G1196600.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010269
Physical Location & Seq
Reverse (-)
17078140 .. 17081064
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1196600.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGAGTCCATCTTCGGCTTGGTAGGCGATGGCTTTGCCGTCGTGGCGGCGGACACTTCAGCCGTGCATAGCATACTGGTGCACAAGTCCAACGAGGACAAGATCATGGTGCTCGACTCCCACAAGCTCGTCGCCGCCAGCGGCGAGCCCGGCGACAGGGTTCAGTTTACGGAGTACATACAGAAGAACGTGGCTTTGTACCAGTTCCGGAATGGGATTCCGTTGACCACCGCTGCTGCTGCCAATTTCACCCGCGGCGAGCTTGCCACCGCCTTGCGAAAGAATCCGTACATGGTGAACATCTTGATGGCTGGTTTTGATAAGGAGGCGGGGGCATCCCTTTACTACATCGATTACATTGCCTCTCTTCACAAGGTTGAGAAGGGAGCCTTCGGATATGGGTCGTACTTTGCGCTCTCACTGATGGACAGGCACTACCACAGTGGCATGTCCGTGGAGGAAGCAATTGACTTGGTTGACAAGTGCATCATCGAGATCCGGTCAAGACTAGTGGTGGCTCCCCCAAACTTTGTGATCAAGATTGTTGACAAGGACGGGGCAAGGGAGTATGCATGGCGGGAGTCCATCAGAGATATCAACGCTGCTGCTGCATGA

Protein Analysis

205

Amino Acids

22.41

Weight (kDa)

5.86

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 3 - 182 3e-41 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22630 AT4G14800 AT4G14800
fragaria_vesca FvH4_4g15960 FvH4_7g08790
malus_domestica MD13G1196600.v1.1 MD16G1197000.v1.1
prunus_persica Prupe.1G017400_v2.0.a1 Prupe.1G017400_v2.0.a1
pyrus_communis pycom01g08470 pycom16g16580
rosa_chinensis RchiOBHm_Chr4g0415561
rosa_laevigata RLG00000008063
rosa_multiflora Rmu_sc0004599.1_g000004
rosa_roxburghii Rroxscaffold_5G00359380
rosa_rugosa Rorug04G0134700
rosa_samantha Rh4AG196100 Rh4BG194800 Rh4CG206800 Rh4DG193600
rosa_wichuraiana Rw4G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AccIII TCCGGA 1 cut(s) 207
AclWI GGATC 1 cut(s) 490
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 4 cut(s) 176, 200, 290, 407
AfiI CCNNNNNNNGG 1 cut(s) 156
AhlI ACTAGT 1 cut(s) 508
AluBI AGCT 2 cut(s) 127, 262
AluI AGCT 2 cut(s) 127, 262
Alw21I GWGCWC 2 cut(s) 84, 114
Alw44I GTGCAC 1 cut(s) 80
AlwI GGATC 1 cut(s) 490
Aor13HI TCCGGA 1 cut(s) 207
ApaLI GTGCAC 1 cut(s) 80
ApeKI GCWGC 6 cut(s) 233, 236, 239, 602, 605, 608
AspLEI GCGC 1 cut(s) 415
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 241, 307
BaeGI GKGCMC 1 cut(s) 84
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 2 cut(s) 84, 114
BbvI GCAGC 6 cut(s) 220, 223, 226, 589, 592, 595
BccI CCATC 5 cut(s) 17, 23, 301, 418, 593
BceAI ACGGC 2 cut(s) 23, 47
BcgI CGANNNNNNTGC 4 cut(s) 17, 51, 341, 375
BclI TGATCA 1 cut(s) 534
BcnI CCSGG 1 cut(s) 150
BcuI ACTAGT 1 cut(s) 508
BfaI CTAG 1 cut(s) 509
BglI GCCNNNNNGGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 150
BmiI GGNNCC 2 cut(s) 388, 519
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 2 cut(s) 344, 495
BpuMI CCSGG 1 cut(s) 150
Bsa29I ATCGAT 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 253, 453
BsaWI WCCGGW 2 cut(s) 207, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 81, 202
Bse3DI GCAATG 1 cut(s) 357
BseAI TCCGGA 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 351
BseDI CCNNGG 2 cut(s) 253, 453
BseGI GGATG 1 cut(s) 335
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 81, 202
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 6 cut(s) 220, 223, 226, 589, 592, 595
Bsh1236I CGCG 1 cut(s) 255
BshVI ATCGAT 1 cut(s) 351
BsiHKAI GWGCWC 2 cut(s) 84, 114
BsiSI CCGG 3 cut(s) 150, 208, 499
BslI CCNNNNNNNGG 1 cut(s) 156
Bsp1286I GDGCHC 3 cut(s) 84, 114, 150
Bsp13I TCCGGA 1 cut(s) 207
Bsp143I GATC 3 cut(s) 102, 495, 534
BspDI ATCGAT 1 cut(s) 351
BspEI TCCGGA 1 cut(s) 207
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 388, 519
BspPI GGATC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 2 cut(s) 81, 202
BssECI CCNNGG 2 cut(s) 253, 453
BssMI GATC 3 cut(s) 102, 495, 534
Bst4CI ACNGT 1 cut(s) 443
Bst6I CTCTTC 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 139, 146, 260, 264
BstDSI CCRYGG 2 cut(s) 253, 453
BstF5I GGATG 1 cut(s) 335
BstFNI CGCG 1 cut(s) 255
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 3 cut(s) 105, 498, 537
BstMBI GATC 3 cut(s) 102, 495, 534
BstMWI GCNNNNNNNGC 5 cut(s) 24, 44, 150, 239, 608
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 84
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 6 cut(s) 220, 223, 226, 589, 592, 595
BstX2I RGATCY 1 cut(s) 495
BstYI RGATCY 1 cut(s) 495
Bsu15I ATCGAT 1 cut(s) 351
BsuTUI ATCGAT 1 cut(s) 351
BtgI CCRYGG 2 cut(s) 253, 453
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 335
BtsIMutI CAGTG 2 cut(s) 419, 448
Cac8I GCNNGC 4 cut(s) 139, 146, 260, 264
CfoI GCGC 1 cut(s) 415
Cfr42I CCGCGG 1 cut(s) 256
ClaI ATCGAT 1 cut(s) 351
Csp6I GTAC 4 cut(s) 175, 199, 289, 406
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviAII CATG 5 cut(s) 106, 292, 448, 573, 612
CviQI GTAC 4 cut(s) 175, 199, 289, 406
DpnI GATC 3 cut(s) 104, 497, 536
DpnII GATC 3 cut(s) 102, 495, 534
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 42
EcoRV GATATC 1 cut(s) 595
EcoT22I ATGCAT 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 5 cut(s) 109, 295, 451, 576, 615
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FatI CATG 5 cut(s) 105, 291, 447, 572, 611
FauI CCCGC 3 cut(s) 260, 322, 570
FbaI TGATCA 1 cut(s) 534
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 1 cut(s) 150
FspBI CTAG 1 cut(s) 509
GlaI GCGC 1 cut(s) 414
HapII CCGG 3 cut(s) 150, 208, 499
HhaI GCGC 1 cut(s) 415
Hin1II CATG 5 cut(s) 109, 295, 451, 576, 615
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HincII GTYRAC 3 cut(s) 225, 478, 547
HindII GTYRAC 3 cut(s) 225, 478, 547
HinfI GANTC 5 cut(s) 5, 116, 217, 283, 581
HpaII CCGG 3 cut(s) 150, 208, 499
HphI GGTGA 2 cut(s) 241, 307
Hpy166II GTNNAC 6 cut(s) 82, 168, 225, 298, 478, 547
Hpy188I TCNGA 2 cut(s) 395, 590
Hpy188III TCNNGA 5 cut(s) 208, 304, 493, 504, 538
Hpy8I GTNNAC 6 cut(s) 82, 168, 225, 298, 478, 547
Hpy99I CGWCG 2 cut(s) 44, 134
HpyAV CCTTC 2 cut(s) 376, 400
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 5 cut(s) 67, 82, 486, 572, 611
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 44, 150, 239, 608
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 5 cut(s) 109, 295, 451, 576, 615
HspAI GCGC 1 cut(s) 413
Kpn2I TCCGGA 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 534
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 3 cut(s) 102, 495, 534
LmnI GCTCC 2 cut(s) 386, 523
LpnPI CCDG 9 cut(s) 62, 142, 151, 163, 215, 221, 297, 415, 512
Lsp1109I GCAGC 6 cut(s) 220, 223, 226, 589, 592, 595
LweI GCATC 2 cut(s) 344, 495
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 189
MalI GATC 3 cut(s) 104, 497, 536
MboI GATC 3 cut(s) 102, 495, 534
MboII GAAGA 3 cut(s) 4, 196, 359
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 3 cut(s) 84, 114, 150
MluCI AATT 2 cut(s) 244, 465
MlyI GAGTC 3 cut(s) 14, 110, 590
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 88, 319, 373, 451
Mph1103I ATGCAT 1 cut(s) 574
MroI TCCGGA 1 cut(s) 207
MslI CAYNNNNRTG 3 cut(s) 77, 305, 452
MspA1I CMGCKG 3 cut(s) 141, 233, 255
MspI CCGG 3 cut(s) 150, 208, 499
MspR9I CCNGG 1 cut(s) 150
MunI CAATTG 1 cut(s) 465
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 24, 44, 150, 239, 608
NciI CCSGG 1 cut(s) 150
NdeII GATC 3 cut(s) 102, 495, 534
NlaIII CATG 5 cut(s) 109, 295, 451, 576, 615
NlaIV GGNNCC 2 cut(s) 388, 519
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 1 cut(s) 451
PfeI GAWTC 2 cut(s) 217, 283
PleI GAGTC 3 cut(s) 13, 110, 589
PpsI GAGTC 3 cut(s) 13, 110, 589
PspN4I GGNNCC 2 cut(s) 388, 519
PsuI RGATCY 1 cut(s) 495
RsaI GTAC 4 cut(s) 176, 200, 290, 407
RsaNI GTAC 4 cut(s) 175, 199, 289, 406
RseI CAYNNNNRTG 3 cut(s) 77, 305, 452
SacII CCGCGG 1 cut(s) 256
Sau3AI GATC 3 cut(s) 102, 495, 534
SchI GAGTC 3 cut(s) 14, 110, 590
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 3 cut(s) 84, 114, 150
SetI ASST 4 cut(s) 129, 192, 264, 378
SfaNI GCATC 2 cut(s) 344, 495
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SmiMI CAYNNNNRTG 3 cut(s) 77, 305, 452
SpeI ACTAGT 1 cut(s) 508
Sse9I AATT 2 cut(s) 244, 465
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 443
TaiI ACGT 1 cut(s) 192
TaqI TCGA 3 cut(s) 114, 351, 492
TasI AATT 2 cut(s) 244, 465
TatI WGTACW 1 cut(s) 174
TauI GCSGC 4 cut(s) 50, 137, 144, 258
TfiI GAWTC 2 cut(s) 217, 283
TscAI CASTG 2 cut(s) 426, 448
TseI GCWGC 6 cut(s) 233, 236, 239, 602, 605, 608
TspGWI ACGGA 4 cut(s) 185, 210, 276, 442
TspRI CASTG 2 cut(s) 426, 448
VneI GTGCAC 1 cut(s) 80
XceI RCATGY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 2 cut(s) 16, 209
XspI CTAG 1 cut(s) 509
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.