Rh4BG194800

Proteasome subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010269
Physical Location & Seq
Forward (+)
33631745 .. 33634817
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG194800.1

Sequence Viewer

Length: 615 bp
ATGGAGTCTATCTTCGGTTTGGTTGGGAACGATTTCGCCATAGTGGCGGCGGACACGTCGGCGGTTCACAGCATACTGGTGCACAAGAACAACGAGGACAAGATCATGGTGCTTGACTCGCACAAGCTAATCGCGGCCAGCGGTGAGCCCGGAGACAGGGTTCAGTTCACGGAGTACATCCAGAAGAACGTGTCGCTCTATCAGTTTCGCAATGGGATTCCTCTGACCACTGCCGCCGCAGCTAATTTCACCCGCGGTGAGCTCGCCACCGCCTTGCGTAAGAACCCATACCATGTGAACATTCTCATGGCTGGATATGATAAAGAGAAGGGGGCAGAACTGTACTACATTGATTACATTGCTTCCCTCCACAAGGTTGAAAAGGGCGCCTTTGGTTACGGGTCCTATTTTGCTCTCTCCCTTATGGATCGACTCTATCACAAAGGTATGTCTGTTGAAGAAGCCATTGCCTTAGTTGACAAGATCATTATTGAGATCCGATCAAGGCTCGTCGTTGCTCCACCTAACTTTGTCATCAAGATAGTGGACAAGGATGGAGCTAGGGAGTATGCGTGGCGTGAGTCCATCAAGGATGCTGGTGTGGCTGGTGCTTGA

Protein Analysis

204

Amino Acids

22.53

Weight (kDa)

6.31

Isoelectric Point (pI)

23.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 3 - 182 9.1e-41 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22630 AT4G14800 AT4G14800
fragaria_vesca FvH4_4g15960 FvH4_7g08790
malus_domestica MD13G1196600.v1.1 MD16G1197000.v1.1
prunus_persica Prupe.1G017400_v2.0.a1 Prupe.1G017400_v2.0.a1
pyrus_communis pycom01g08470 pycom16g16580
rosa_chinensis RchiOBHm_Chr4g0415561
rosa_laevigata RLG00000008063
rosa_multiflora Rmu_sc0004599.1_g000004
rosa_roxburghii Rroxscaffold_5G00359380
rosa_rugosa Rorug04G0134700
rosa_samantha Rh4AG196100 Rh4BG194800 Rh4CG206800 Rh4DG193600
rosa_wichuraiana Rw4G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 386
AccII CGCG 2 cut(s) 134, 255
AclWI GGATC 2 cut(s) 435, 490
AcoI YGGCCR 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 2 cut(s) 176, 344
AfiI CCNNNNNNNGG 2 cut(s) 156, 373
AflIII ACRYGT 2 cut(s) 54, 189
AgsI TTSAA 2 cut(s) 380, 458
AjiI CACGTC 1 cut(s) 57
AloI GAACNNNNNNTCC 2 cut(s) 144, 176
AluBI AGCT 4 cut(s) 127, 242, 262, 560
AluI AGCT 4 cut(s) 127, 242, 262, 560
Alw21I GWGCWC 2 cut(s) 84, 264
Alw26I GTCTC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 80
AlwI GGATC 2 cut(s) 435, 490
AoxI GGCC 1 cut(s) 135
ApaLI GTGCAC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 239
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 402
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 155, 241, 269
AvaII GGWCC 1 cut(s) 402
BaeGI GKGCMC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 2 cut(s) 150, 264
Bbv12I GWGCWC 2 cut(s) 84, 264
BbvI GCAGC 1 cut(s) 251
BccI CCATC 2 cut(s) 548, 593
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 390
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 5 cut(s) 48, 135, 234, 237, 240
BlsI GCNGC 5 cut(s) 49, 136, 235, 238, 241
Bme1390I CCNGG 1 cut(s) 150
Bme18I GGWCC 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 402
BmiI GGNNCC 2 cut(s) 388, 403
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 583
BpuMI CCSGG 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 253
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 557, 587
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 373
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 3 cut(s) 217, 357, 465
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 3 cut(s) 177, 559, 598
BseLI CCNNNNNNNGG 2 cut(s) 156, 373
BseMI GCAATG 3 cut(s) 217, 357, 465
BseNI ACTGG 1 cut(s) 81
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 1 cut(s) 251
Bsh1236I CGCG 2 cut(s) 134, 255
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 386
BsiHKAI GWGCWC 2 cut(s) 84, 264
BsiSI CCGG 1 cut(s) 150
BslI CCNNNNNNNGG 2 cut(s) 156, 373
BsmAI GTCTC 1 cut(s) 147
BsnI GGCC 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 84, 150, 264
Bsp143I GATC 5 cut(s) 102, 427, 483, 495, 500
BspANI GGCC 1 cut(s) 137
BspFNI CGCG 2 cut(s) 134, 255
BspLI GGNNCC 2 cut(s) 388, 403
BspPI GGATC 2 cut(s) 435, 490
BspT107I GGYRCC 1 cut(s) 386
BsrDI GCAATG 3 cut(s) 217, 357, 465
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 5 cut(s) 102, 427, 483, 495, 500
BssNI GRCGYC 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 139, 264
BstDEI CTNAG 1 cut(s) 472
BstDSI CCRYGG 1 cut(s) 253
BstENI CCTNNNNNAGG 1 cut(s) 371
BstF5I GGATG 3 cut(s) 177, 559, 598
BstFNI CGCG 2 cut(s) 134, 255
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 5 cut(s) 105, 430, 486, 498, 503
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 5 cut(s) 102, 427, 483, 495, 500
BstMWI GCNNNNNNNGC 4 cut(s) 44, 118, 239, 602
BstSCI CCNGG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 84
BstUI CGCG 2 cut(s) 134, 255
BstV1I GCAGC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 495
BstYI RGATCY 1 cut(s) 495
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 253
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 3 cut(s) 177, 559, 598
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 2 cut(s) 139, 264
CfoI GCGC 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 402
Cfr42I CCGCGG 1 cut(s) 256
Csp6I GTAC 2 cut(s) 175, 343
CviAII CATG 3 cut(s) 106, 293, 307
CviQI GTAC 2 cut(s) 175, 343
DdeI CTNAG 1 cut(s) 472
DinI GGCGCC 1 cut(s) 388
DpnI GATC 5 cut(s) 104, 429, 485, 497, 502
DpnII GATC 5 cut(s) 102, 427, 483, 495, 500
EaeI YGGCCR 1 cut(s) 135
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 2 cut(s) 150, 264
Eco47I GGWCC 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoNI CCTNNNNNAGG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 402
EcoT38I GRGCYC 2 cut(s) 150, 264
EgeI GGCGCC 1 cut(s) 388
EheI GGCGCC 1 cut(s) 388
FaeI CATG 3 cut(s) 109, 296, 310
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FatI CATG 3 cut(s) 105, 292, 306
FauI CCCGC 1 cut(s) 260
Fnu4HI GCNGC 5 cut(s) 48, 135, 234, 237, 240
FokI GGATG 3 cut(s) 164, 566, 605
FriOI GRGCYC 2 cut(s) 150, 264
Fsp4HI GCNGC 5 cut(s) 48, 135, 234, 237, 240
FspBI CTAG 1 cut(s) 561
GlaI GCGC 1 cut(s) 388
GluI GCNGC 5 cut(s) 48, 135, 234, 237, 240
HaeII RGCGCY 1 cut(s) 390
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 150
HhaI GCGC 1 cut(s) 389
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 3 cut(s) 109, 296, 310
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 5 cut(s) 5, 116, 217, 432, 581
HpaII CCGG 1 cut(s) 150
HphI GGTGA 3 cut(s) 155, 241, 269
Hpy166II GTNNAC 6 cut(s) 67, 82, 168, 298, 478, 547
Hpy188I TCNGA 2 cut(s) 225, 500
Hpy188III TCNNGA 2 cut(s) 181, 538
Hpy8I GTNNAC 6 cut(s) 67, 82, 168, 298, 478, 547
Hpy99I CGWCG 2 cut(s) 61, 515
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 2 cut(s) 56, 189
HpyCH4V TGCA 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 118, 239, 602
HpyF3I CTNAG 1 cut(s) 472
HpySE526I ACGT 2 cut(s) 56, 189
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 3 cut(s) 109, 296, 310
HspAI GCGC 1 cut(s) 387
KasI GGCGCC 1 cut(s) 386
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 5 cut(s) 102, 427, 483, 495, 500
LmnI GCTCC 2 cut(s) 523, 557
LpnPI CCDG 8 cut(s) 62, 142, 151, 163, 194, 297, 582, 591
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 1 cut(s) 583
MaeI CTAG 1 cut(s) 561
MaeII ACGT 2 cut(s) 56, 189
MaeIII GTNAC 1 cut(s) 395
MalI GATC 5 cut(s) 104, 429, 485, 497, 502
MboI GATC 5 cut(s) 102, 427, 483, 495, 500
MboII GAAGA 3 cut(s) 4, 196, 470
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 3 cut(s) 84, 150, 264
MluCI AATT 1 cut(s) 244
Mly113I GGCGCC 1 cut(s) 387
MlyI GAGTC 4 cut(s) 14, 110, 426, 590
MnlI CCTC 3 cut(s) 88, 231, 377
MroXI GAANNNNTTC 1 cut(s) 32
MslI CAYNNNNRTG 2 cut(s) 77, 305
MspA1I CMGCKG 2 cut(s) 141, 255
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 1 cut(s) 150
MvnI CGCG 2 cut(s) 134, 255
MwoI GCNNNNNNNGC 4 cut(s) 44, 118, 239, 602
NarI GGCGCC 1 cut(s) 387
NciI CCSGG 1 cut(s) 150
NdeII GATC 5 cut(s) 102, 427, 483, 495, 500
NlaIII CATG 3 cut(s) 109, 296, 310
NlaIV GGNNCC 2 cut(s) 388, 403
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 217
PkrI GCNGC 5 cut(s) 49, 136, 235, 238, 241
PleI GAGTC 4 cut(s) 13, 110, 426, 589
PluTI GGCGCC 1 cut(s) 390
PpsI GAGTC 4 cut(s) 13, 110, 426, 589
PpuMI RGGWCCY 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 402
PspN4I GGNNCC 2 cut(s) 388, 403
PspPI GGNCC 1 cut(s) 402
PspPPI RGGWCCY 1 cut(s) 402
PsuI RGATCY 1 cut(s) 495
RsaI GTAC 2 cut(s) 176, 344
RsaNI GTAC 2 cut(s) 175, 343
RseI CAYNNNNRTG 2 cut(s) 77, 305
SacI GAGCTC 1 cut(s) 264
SacII CCGCGG 1 cut(s) 256
SatI GCNGC 5 cut(s) 48, 135, 234, 237, 240
Sau3AI GATC 5 cut(s) 102, 427, 483, 495, 500
Sau96I GGNCC 1 cut(s) 402
SchI GAGTC 4 cut(s) 14, 110, 426, 590
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 3 cut(s) 84, 150, 264
SetI ASST 9 cut(s) 59, 129, 192, 244, 264, 378, 448, 526, 562
SfaNI GCATC 1 cut(s) 583
SfoI GGCGCC 1 cut(s) 388
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SinI GGWCC 1 cut(s) 402
SmiMI CAYNNNNRTG 2 cut(s) 77, 305
Sse9I AATT 1 cut(s) 244
SspDI GGCGCC 1 cut(s) 386
SspMI CTAG 1 cut(s) 561
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 2 cut(s) 59, 192
TaqI TCGA 1 cut(s) 430
TasI AATT 1 cut(s) 244
TatI WGTACW 2 cut(s) 174, 342
TauI GCSGC 4 cut(s) 50, 137, 236, 239
TfiI GAWTC 1 cut(s) 217
TscAI CASTG 1 cut(s) 235
TseI GCWGC 1 cut(s) 239
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 1 cut(s) 235
VneI GTGCAC 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 402
XagI CCTNNNNNAGG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.