FvH4_4g15960

Proteasome subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010269
Physical Location & Seq
Forward (+)
19902453 .. 19906327
3875 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15960.t1

Sequence Viewer

Length: 660 bp
ATGGAGTCAATCTTCGGGTTGGTTGGGAACAACTTCGCCATAGTGGCAGCGGACACGTCGGCGGTGCACAGCATACTGGTGCACAAGACCAACGAGGACAAGATCATGGTCCTCGATTCCCACAAGCTCATCGCCGCCAGCGGTGAGCCCGGCGACAGGGTTCAGTTCACGGAGTACATCCAGAAGAACGTTTCGCTCTATCAGTTTCGTAATGGGATTCCTCTTACCACCGCCGCCGCTGCCAATTTCACCCGCGGTGAGCTCGCCACCGCTTTGCGCAAGAACCCATACCATGTGAACATTCTCATGGCTGGATATGATAAAGAGAAAGGCGCAGAACTGTACTACATTGATTACATTGCTTCCCTCCACAAGGTTGACAAGGGCGCCTTTGGTTATGGTTCCTATTTTGCTCTCTCCCTCATGGATCGACTCTATCACAAAGGTATGTCTGTTGATGAGGCTATTGCCTTAATTGACAAGATTATCATTGAGATACGATCAAGGCTTGTTGTCGCTCCACCCAACTTTGTCATCAAGTTAGTGGACAAGGATGGAGCTAGGGAGTATGCATGGCGTGAGTCCATCAAGGATGCTGGTGTGGCTGCTGCCTGCTTGAGGCGACTTTATTGGCTCAACAACCCATCAGATCCCAAATGA

Protein Analysis

220

Amino Acids

24.37

Weight (kDa)

7.8

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 3 - 180 1.4e-39 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22630 AT4G14800 AT4G14800
fragaria_vesca FvH4_4g15960 FvH4_7g08790
malus_domestica MD13G1196600.v1.1 MD16G1197000.v1.1
prunus_persica Prupe.1G017400_v2.0.a1 Prupe.1G017400_v2.0.a1
pyrus_communis pycom01g08470 pycom16g16580
rosa_chinensis RchiOBHm_Chr4g0415561
rosa_laevigata RLG00000008063
rosa_multiflora Rmu_sc0004599.1_g000004
rosa_roxburghii Rroxscaffold_5G00359380
rosa_rugosa Rorug04G0134700
rosa_samantha Rh4AG196100 Rh4BG194800 Rh4CG206800 Rh4DG193600
rosa_wichuraiana Rw4G016670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 386
AccII CGCG 1 cut(s) 255
AclI AACGTT 1 cut(s) 189
AclWI GGATC 2 cut(s) 435, 644
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 2 cut(s) 176, 344
AfiI CCNNNNNNNGG 3 cut(s) 156, 373, 618
AflIII ACRYGT 1 cut(s) 54
AjiI CACGTC 1 cut(s) 57
AluBI AGCT 3 cut(s) 127, 262, 560
AluI AGCT 3 cut(s) 127, 262, 560
Alw21I GWGCWC 3 cut(s) 69, 84, 264
Alw44I GTGCAC 2 cut(s) 65, 80
AlwI GGATC 2 cut(s) 435, 644
ApaLI GTGCAC 2 cut(s) 65, 80
ApeKI GCWGC 4 cut(s) 47, 239, 605, 608
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 3 cut(s) 279, 335, 389
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 3 cut(s) 155, 241, 269
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 2 cut(s) 69, 84
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 2 cut(s) 150, 264
Bbv12I GWGCWC 3 cut(s) 69, 84, 264
BbvI GCAGC 4 cut(s) 59, 226, 592, 595
BccI CCATC 3 cut(s) 548, 593, 652
BcnI CCSGG 1 cut(s) 150
BfaI CTAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 390
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 7 cut(s) 48, 135, 234, 237, 240, 606, 609
BlsI GCNGC 7 cut(s) 49, 136, 235, 238, 241, 607, 610
Bme1390I CCNGG 1 cut(s) 150
Bme18I GGWCC 1 cut(s) 109
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 2 cut(s) 388, 403
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 637
BpuMI CCSGG 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 253
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 373, 618
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 3 cut(s) 177, 559, 598
BseLI CCNNNNNNNGG 3 cut(s) 156, 373, 618
BseMI GCAATG 1 cut(s) 357
BseNI ACTGG 1 cut(s) 81
BseSI GKGCMC 2 cut(s) 69, 84
BseXI GCAGC 4 cut(s) 59, 226, 592, 595
Bsh1236I CGCG 1 cut(s) 255
BshNI GGYRCC 1 cut(s) 386
BsiHKAI GWGCWC 3 cut(s) 69, 84, 264
BsiSI CCGG 1 cut(s) 150
BslI CCNNNNNNNGG 3 cut(s) 156, 373, 618
Bsp1286I GDGCHC 4 cut(s) 69, 84, 150, 264
Bsp143I GATC 4 cut(s) 102, 427, 500, 649
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 388, 403
BspPI GGATC 2 cut(s) 435, 644
BspT107I GGYRCC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 357
BsrI ACTGG 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 4 cut(s) 102, 427, 500, 649
BssNI GRCGYC 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 3 cut(s) 139, 264, 613
BstDSI CCRYGG 1 cut(s) 253
BstENI CCTNNNNNAGG 2 cut(s) 371, 616
BstF5I GGATG 3 cut(s) 177, 559, 598
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 3 cut(s) 279, 335, 389
BstKTI GATC 4 cut(s) 105, 430, 503, 652
BstMBI GATC 4 cut(s) 102, 427, 500, 649
BstMWI GCNNNNNNNGC 3 cut(s) 44, 239, 602
BstSCI CCNGG 1 cut(s) 148
BstSLI GKGCMC 2 cut(s) 69, 84
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 4 cut(s) 59, 226, 592, 595
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BtgI CCRYGG 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 115
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 3 cut(s) 177, 559, 598
Cac8I GCNNGC 3 cut(s) 139, 264, 613
CfoI GCGC 3 cut(s) 279, 335, 389
Cfr13I GGNCC 1 cut(s) 109
Cfr42I CCGCGG 1 cut(s) 256
Csp6I GTAC 2 cut(s) 175, 343
CviAII CATG 5 cut(s) 106, 293, 307, 424, 573
CviJI RGCY 9 cut(s) 127, 148, 262, 311, 464, 508, 560, 605, 634
CviKI_1 RGCY 9 cut(s) 127, 148, 262, 311, 464, 508, 560, 605, 634
CviQI GTAC 2 cut(s) 175, 343
DinI GGCGCC 1 cut(s) 388
DpnI GATC 4 cut(s) 104, 429, 502, 651
DpnII GATC 4 cut(s) 102, 427, 500, 649
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 2 cut(s) 150, 264
Eco47I GGWCC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 262
EcoNI CCTNNNNNAGG 2 cut(s) 371, 616
EcoT22I ATGCAT 1 cut(s) 574
EcoT38I GRGCYC 2 cut(s) 150, 264
EgeI GGCGCC 1 cut(s) 388
EheI GGCGCC 1 cut(s) 388
FaeI CATG 5 cut(s) 109, 296, 310, 427, 576
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FatI CATG 5 cut(s) 105, 292, 306, 423, 572
FauI CCCGC 1 cut(s) 260
Fnu4HI GCNGC 7 cut(s) 48, 135, 234, 237, 240, 606, 609
FokI GGATG 3 cut(s) 164, 566, 605
FriOI GRGCYC 2 cut(s) 150, 264
Fsp4HI GCNGC 7 cut(s) 48, 135, 234, 237, 240, 606, 609
FspBI CTAG 1 cut(s) 561
FspI TGCGCA 1 cut(s) 278
GlaI GCGC 3 cut(s) 278, 334, 388
GluI GCNGC 7 cut(s) 48, 135, 234, 237, 240, 606, 609
HaeII RGCGCY 1 cut(s) 390
HapII CCGG 1 cut(s) 150
HhaI GCGC 3 cut(s) 279, 335, 389
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 5 cut(s) 109, 296, 310, 427, 576
Hin6I GCGC 3 cut(s) 277, 333, 387
HinP1I GCGC 3 cut(s) 277, 333, 387
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 5 cut(s) 5, 116, 217, 432, 581
HpaII CCGG 1 cut(s) 150
HphI GGTGA 3 cut(s) 155, 241, 269
Hpy166II GTNNAC 6 cut(s) 67, 82, 168, 298, 379, 547
Hpy188I TCNGA 1 cut(s) 649
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 6 cut(s) 67, 82, 168, 298, 379, 547
Hpy99I CGWCG 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 2 cut(s) 56, 189
HpyCH4V TGCA 3 cut(s) 67, 82, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 239, 602
HpySE526I ACGT 2 cut(s) 56, 189
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 5 cut(s) 109, 296, 310, 427, 576
HspAI GCGC 3 cut(s) 277, 333, 387
KasI GGCGCC 1 cut(s) 386
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 4 cut(s) 102, 427, 500, 649
LmnI GCTCC 2 cut(s) 523, 557
LpnPI CCDG 8 cut(s) 62, 142, 151, 163, 194, 297, 582, 625
Lsp1109I GCAGC 4 cut(s) 59, 226, 592, 595
LweI GCATC 1 cut(s) 583
MaeI CTAG 1 cut(s) 561
MaeII ACGT 2 cut(s) 56, 189
MalI GATC 4 cut(s) 104, 429, 502, 651
MboI GATC 4 cut(s) 102, 427, 500, 649
MboII GAAGA 2 cut(s) 4, 196
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 4 cut(s) 69, 84, 150, 264
MluCI AATT 2 cut(s) 244, 474
Mly113I GGCGCC 1 cut(s) 387
MlyI GAGTC 3 cut(s) 14, 426, 590
MnlI CCTC 7 cut(s) 88, 122, 231, 377, 431, 454, 612
Mph1103I ATGCAT 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 2 cut(s) 77, 305
MspA1I CMGCKG 4 cut(s) 50, 141, 239, 255
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 3 cut(s) 44, 239, 602
NarI GGCGCC 1 cut(s) 387
NciI CCSGG 1 cut(s) 150
NdeII GATC 4 cut(s) 102, 427, 500, 649
NlaIII CATG 5 cut(s) 109, 296, 310, 427, 576
NlaIV GGNNCC 2 cut(s) 388, 403
NsbI TGCGCA 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 2 cut(s) 116, 217
PkrI GCNGC 7 cut(s) 49, 136, 235, 238, 241, 607, 610
PleI GAGTC 3 cut(s) 13, 426, 589
PluTI GGCGCC 1 cut(s) 390
PpsI GAGTC 3 cut(s) 13, 426, 589
Psp124BI GAGCTC 1 cut(s) 264
Psp1406I AACGTT 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 388, 403
PspPI GGNCC 1 cut(s) 109
PsuI RGATCY 1 cut(s) 649
RsaI GTAC 2 cut(s) 176, 344
RsaNI GTAC 2 cut(s) 175, 343
RseI CAYNNNNRTG 2 cut(s) 77, 305
SacI GAGCTC 1 cut(s) 264
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 7 cut(s) 48, 135, 234, 237, 240, 606, 609
Sau3AI GATC 4 cut(s) 102, 427, 500, 649
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 3 cut(s) 14, 426, 590
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 4 cut(s) 69, 84, 150, 264
SetI ASST 7 cut(s) 59, 129, 192, 264, 378, 448, 562
SfaNI GCATC 1 cut(s) 583
SfoI GGCGCC 1 cut(s) 388
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 2 cut(s) 77, 305
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
Sse9I AATT 2 cut(s) 244, 474
SspDI GGCGCC 1 cut(s) 386
SspMI CTAG 1 cut(s) 561
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 2 cut(s) 59, 192
TaqI TCGA 2 cut(s) 114, 430
TasI AATT 2 cut(s) 244, 474
TatI WGTACW 2 cut(s) 174, 342
TauI GCSGC 3 cut(s) 137, 236, 239
TfiI GAWTC 2 cut(s) 116, 217
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TseI GCWGC 4 cut(s) 47, 239, 605, 608
TspGWI ACGGA 1 cut(s) 185
VneI GTGCAC 2 cut(s) 65, 80
VpaK11BI GGWCC 1 cut(s) 109
XagI CCTNNNNNAGG 2 cut(s) 371, 616
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 561
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.