AT3G24715

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009051
Physical Location & Seq
Forward (+)
9024943 .. 9030311
5369 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24715.2

Sequence Viewer

Length: 3354 bp
ATGTCAGATAGATGGGCTCGACAAAACGCGGAACGTCCTGCAACTACTTTGGCTGAGAGAAGAAATGTGAATAAAAACGTTTCACTTCAGACTGGTGAAGAGTTTTCAATGGAGTTTCTTAAGGATCATACTCCTGTCCAATCTCCTATCGTTGCAGGAAGAACTCATAATGATGTGCATAGATTTGGAGACCTTTACTATCAGAATCAACCACAGGGGTATGATAGTGCTGCTCGGTTTCATGAACTGAGGAGGATAGAATCTGAATGTCCTTCTGATGCATATGATTTTGGTCGAGACCCGAGGAGTACTATACGGCTTGAGAATGGCGGTTATATGCCTCATTTTAATGCTTACCACAATGTAGGTTGTGATAAAGAAGTTATTACTAGGAAAGCTTTTGGTGAAATAAATTCTAACCGAGGAGATGTCACGGGGATGTCTGCGCCTTGCGTGTTCTTGCCAGAGCGTACTCAGTCCAACAACTTTACCGGAGGAGGAGGAGATTTTGATAGATTCGGAAAAGTAAAGTTTCTTTGTAGCTTTGGTGGTAGAATTATGCCAAGATCCACTGATGAAAAACTGAAATACGTTGGAGGAGAGACGCATATTATTTCGATACGCAAGAATCTTTCGTGGGAAGAACTCAAGAAGAAGACCTCAGCTATATGCCAACAGTTGCATTCAATTAAGTATCAGCTTCCAGGAGATGAGCTTGACTCCTTGATATCAGTATCTTCGGATGAGGATCTTCAGAATATGATTGAAGAGTACAATGGACTTGAAAGGCTTGAAGGTTCACAACGGCCACGACTGTTCTTGATTCCTATTGGAGAGCCTGAAAGAAAGGCTCAGCAGAACATTCCTGATTGCCAATATGCTGCAGCTTTAAATTGTAATGCTGATCCTAATCCTAGGAACATTTCTGATGGTCAGACTTTGGCTGGTGATTCACGTTATCATATAAACAATTTGGATCGTAATCCAAGTTTCTGTAAACGAACACCGGGTCAGATGCTTCGACTGGATGCTACTGCTATGCGTCCTAATCCACTTTTCAATGGAGTTCAGTATAACATGGCTTCATATCCATCACCTCCTGTTTCTCCTTCACCATTCCAGCAAAGGGATTCAAATGGTGCATACTCACAGTTCCATGGCAACAACTCCAGCTCGGAGAGCAATAATTCCTTCACTCCAGCTCAACAAGACACGTCTATCTTCGAGGTGATTGATTCCAAATATCATCAACAGCGACCACTACCTTCAGTTAACTATCAGTCTAACAAGCAGGAGGCAGAGAACCTATATGGGATTCAGTTCCAGAATGGCTTTAATGACAAACCTGTGACTCCCAATCTTGGTCATGTTGATAATCTTTGCTTTAACCCTGAGAGGTCTGCGAATAACGGAAGGGTCTTCTATGATAAAGTGAGCATGCCCGAGGAATCTAAGGTTTCCTTCTCTGGGTCTACAAACTCAAATGATTCTTGCTTGGGAATCCCACACTCGTACTCAGACTCTACATTGGAGATTACTGGTGGACACTCTAGCTACTTTTCACAAGAGAGACAACAAAGCCCATCTTCAACATTGAACTTTACAAAGAAACAAACACAAGAAAAGCAAGTACAGGTGCATAGGAATAACGATCTAGCTGACCGTCGTACACAAAGCGATATACTCGACATGAAGTCCACTGAGGGAGGAGAGACTATGTTTAAATTTTCCCCAAGTCCACGAGGACCTAGACTATCCGGTGAAAACAAGACGTTGCATATAGACATTTCTGCTGCAGGCAATCATTATGATGAAATTTATCTAAATCAGGAAACCAAGAATCAAGGAGGGAATAGTGATACCATTTTTCATCTAGGAGGGAAAGTACTGGGAACAAGAGCAATGCCTACTGACATGGAAGGCAAGAAGTTGCCTATATATGGAAACCAAACCTCAGTTGTTGTTGATCCATGGAAGCAAATAAAGCAAGATAATGAGCGTTTGATGGCTGGAGCTGGAACTTCATCTGCTAATCTAATTTCCTTAGAGGAAGGTATAGCAGCTAATACCCCTAATGAGGAACCTGAATCTCGTGCTGCCCGAGAAAGGAACTTGGAAGTTTCAGGGATCTTTATAAGTAAGAGAGTAGTCTCTGATGAGAATTTCTTGTTCAGCATCGCCTCTGAATCACCAAACAGCAAGATTGTTCATGAGACACAAAAGAATAGAACAGAAAGAGAAGGTGGTTTAGGCCATGTGAGAATCCCAATCATGGATTTGAATCAGCCAGCGCCAGCAACAGCGAGTGAAACTTACAGTTTGAAGACACCAAGAGAAGACTCACCACAAAGCATTCCACATGCAAAGATTACCAGCGGTGATACAATATTCCTTTCTGAGGAAGCTGAAGCCAATACCGGCCAGAAAGAGAACAGTTTCAAAGACACACTCTTTGTTGAAATGGAAGCCAGTGTTTATGGCTTGCAGATAATAAAGAACGCTGATCTTGAAGACCTGACAGAACTGGGATCCGGGACATATGGAACTGTCTATCATGGAACATGGCGGGGAACGGATGTTGCTATAAAGAGAATACGGAACAGTTGCTTTGCAGGGAGATCATCTGAACAAGAACGCTTGACGAAAGATTTCTGGAGGGAAGCACAAATACTCTCAAATCTTCATCACCCAAACGTTGTTGCATTCTACGGTATAGTGCCTGATGGAACTGGAGGAACCTTGGCAACTGTTACAGAGTTCATGGTGAATGGGTCACTCAGGCATGCTCTTCTCAAAAAGGACAGATTGCTTGACACTCGGAAAAAGATAATAATTGCAATGGATGCTGCTTTTGGCATGGAGTATTTGCACTCGAAGAACATCGTCCACTTTGATCTCAAATGCGAAAACTTACTTGTCAATTTAAGGGATCCACAACGGCCTATATGCAAGGTGGGAGATCTTGGCTTATCTAGAATCAAACGTAACACTTTAGTATCTGGGGGAGTGAGAGGAACTCTTCCATGGATGGCCCCAGAACTCTTGAACGGAAGCAGCACCCGAGTTTCTGAGAAAGTTGATGTTTTTTCATATGGAATCTCGCTGTGGGAGATTCTAACCGGAGAGGAACCCTATGCAGATATGCATTGTGGAGCAATCATTGGCGGGATCGTGAAGAACACACTTCGACCTCCTATACCAAAAAGCTGTTCTCCGGAATGGAAAAAGCTGATGGAACAATGCTGGTCTGTCGACCCGGATTCTCGTCCTCCATTCACCGAAATAACATGCCGACTTCGGTCCATGTCAATGGAAGTCGTCACTAAAAGCAAAAGAAGAGAAAATAAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1117

Amino Acids

124.86

Weight (kDa)

6.26

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 192 - 274 1.3e-20 PB1 domain
Pkinase PF00069 838 - 1096 1.8e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 840 - 1099 8.7e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2135
AccI GTMKAC 2 cut(s) 1472, 3252
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 1 cut(s) 3214
AciI CCGC 5 cut(s) 29, 330, 2376, 2566, 3165
AclI AACGTT 2 cut(s) 78, 2694
AcoI YGGCCR 2 cut(s) 806, 2419
AcsI RAATTY 4 cut(s) 412, 1724, 1815, 2161
AcuI CTGAAG 4 cut(s) 71, 737, 1251, 2427
AfaI GTAC 7 cut(s) 310, 472, 773, 1514, 1632, 1669, 1887
AfiI CCNNNNNNNGG 8 cut(s) 1126, 1361, 1467, 1940, 2077, 2106, 2272, 2398
AflII CTTAAG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 1212
AhdI GACNNNNNGTC 1 cut(s) 1693
AjiI CACGTC 1 cut(s) 1215
AjnI CCWGG 1 cut(s) 703
AjuI GAANNNNNNNTTGG 2 cut(s) 3193, 3225
Alw26I GTCTC 7 cut(s) 183, 291, 596, 1564, 1706, 2155, 2207
Ama87I CYCGRG 4 cut(s) 301, 1442, 2100, 3060
Aor13HI TCCGGA 1 cut(s) 3214
AoxI GGCC 5 cut(s) 806, 2251, 2419, 2939, 3030
ApeKI GCWGC 8 cut(s) 230, 881, 884, 1793, 2060, 2096, 2846, 3054
ApoI RAATTY 4 cut(s) 412, 1724, 1815, 2161
ArsI GACNNNNNNTTYG 4 cut(s) 994, 1026, 2435, 2467
Asp700I GAANNNNTTC 2 cut(s) 2435, 2648
AspA2I CCTAGG 1 cut(s) 914
AspLEI GCGC 2 cut(s) 448, 2293
AspS9I GGNCC 3 cut(s) 1745, 3031, 3300
AsuC2I CCSGG 3 cut(s) 1008, 2533, 3257
AvaI CYCGRG 4 cut(s) 301, 1442, 2100, 3060
AvaII GGWCC 2 cut(s) 1745, 3300
AvrII CCTAGG 1 cut(s) 914
BaeI ACNNNNGTAYC 2 cut(s) 2979, 3012
BamHI GGATCC 2 cut(s) 2528, 2929
BanII GRGCYC 1 cut(s) 19
BarI GAAGNNNNNNTAC 2 cut(s) 2652, 2684
BauI CACGAG 2 cut(s) 1740, 2091
BbsI GAAGAC 5 cut(s) 662, 1411, 2330, 2343, 2517
BbvCI CCTCAGC 1 cut(s) 661
BbvI GCAGC 8 cut(s) 217, 868, 896, 1780, 2072, 2083, 2833, 3066
BccI CCATC 8 cut(s) 6, 923, 1099, 1591, 1999, 2717, 3022, 3226
BceAI ACGGC 3 cut(s) 332, 821, 2954
BciT130I CCWGG 1 cut(s) 705
BcnI CCSGG 3 cut(s) 1008, 2533, 3257
BcoDI GTCTC 7 cut(s) 183, 291, 596, 1564, 1706, 2155, 2207
BfaI CTAG 7 cut(s) 390, 915, 1551, 1655, 1749, 1874, 2973
BfmI CTRYAG 2 cut(s) 882, 1794
BfoI RGCGCY 1 cut(s) 2294
BfrI CTTAAG 1 cut(s) 119
BglII AGATCT 1 cut(s) 2959
BisI GCNGC 8 cut(s) 231, 882, 885, 1794, 2061, 2097, 2847, 3055
BlnI CCTAGG 1 cut(s) 914
BlpI GCTNAGC 1 cut(s) 852
BlsI GCNGC 8 cut(s) 232, 883, 886, 1795, 2062, 2098, 2848, 3056
BmcAI AGTACT 2 cut(s) 310, 1887
Bme1390I CCNGG 4 cut(s) 705, 1008, 2533, 3257
Bme18I GGWCC 2 cut(s) 1745, 3300
BmeRI GACNNNNNGTC 1 cut(s) 1693
BmeT110I CYCGRG 4 cut(s) 301, 1442, 2100, 3060
BmgBI CACGTC 1 cut(s) 1215
BmgT120I GGNCC 3 cut(s) 1745, 3031, 3300
BmiI GGNNCC 6 cut(s) 2082, 2530, 2737, 2931, 3033, 3129
BmrFI CCNGG 4 cut(s) 705, 1008, 2533, 3257
BmrI ACTGGG 2 cut(s) 1898, 2534
BmsI GCATC 5 cut(s) 268, 1005, 1018, 2184, 2833
BmuI ACTGGG 2 cut(s) 1898, 2534
BoxI GACNNNNGTC 1 cut(s) 3298
BpiI GAAGAC 5 cut(s) 662, 1411, 2330, 2343, 2517
BplI GAGNNNNNCTC 4 cut(s) 704, 736, 3001, 3033
BpmI CTGGAG 5 cut(s) 1153, 1182, 2031, 2674, 2751
Bpu10I CCTNAGC 1 cut(s) 661
Bpu1102I GCTNAGC 1 cut(s) 852
BpuEI CTTGAG 2 cut(s) 341, 632
BpuMI CCSGG 3 cut(s) 1008, 2533, 3257
BsaBI GATNNNNATC 4 cut(s) 747, 909, 981, 2280
BsaI GGTCTC 2 cut(s) 183, 291
BsaJI CCNNGG 8 cut(s) 302, 421, 914, 1156, 1443, 1970, 2739, 3023
BsaWI WCCGGW 4 cut(s) 491, 1757, 3119, 3214
BsaXI ACNNNNNCTCC 2 cut(s) 1869, 1899
Bsc4I CCNNNNNNNGG 8 cut(s) 1126, 1361, 1467, 1940, 2077, 2106, 2272, 2398
Bse118I RCCGGY 1 cut(s) 2417
Bse1I ACTGG 7 cut(s) 97, 1029, 1543, 1893, 2469, 2529, 2734
Bse3DI GCAATG 2 cut(s) 1908, 2844
Bse8I GATNNNNATC 4 cut(s) 747, 909, 981, 2280
BseAI TCCGGA 1 cut(s) 3214
BseBI CCWGG 1 cut(s) 705
BseDI CCNNGG 8 cut(s) 302, 421, 914, 1156, 1443, 1970, 2739, 3023
BseGI GGATG 6 cut(s) 444, 748, 1033, 2581, 2848, 3033
BseJI GATNNNNATC 4 cut(s) 747, 909, 981, 2280
BseLI CCNNNNNNNGG 8 cut(s) 1126, 1361, 1467, 1940, 2077, 2106, 2272, 2398
BseMI GCAATG 2 cut(s) 1908, 2844
BseNI ACTGG 7 cut(s) 97, 1029, 1543, 1893, 2469, 2529, 2734
BseRI GAGGAG 8 cut(s) 265, 319, 438, 510, 513, 516, 612, 1722
BseXI GCAGC 8 cut(s) 217, 868, 896, 1780, 2072, 2083, 2833, 3066
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 5 cut(s) 808, 2253, 2421, 2941, 3032
BsiHKCI CYCGRG 4 cut(s) 301, 1442, 2100, 3060
BsiSI CCGG 8 cut(s) 492, 1007, 1758, 2418, 2532, 3120, 3215, 3257
BslFI GGGAC 1 cut(s) 2548
BslI CCNNNNNNNGG 8 cut(s) 1126, 1361, 1467, 1940, 2077, 2106, 2272, 2398
BsmAI GTCTC 7 cut(s) 183, 291, 596, 1564, 1706, 2155, 2207
BsmBI CGTCTC 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 2548
BsmI GAATGC 3 cut(s) 682, 2352, 2702
BsnI GGCC 5 cut(s) 808, 2253, 2421, 2941, 3032
Bso31I GGTCTC 2 cut(s) 183, 291
BsoBI CYCGRG 4 cut(s) 301, 1442, 2100, 3060
Bsp1286I GDGCHC 1 cut(s) 19
Bsp13I TCCGGA 1 cut(s) 3214
Bsp1720I GCTNAGC 1 cut(s) 852
Bsp19I CCATGG 3 cut(s) 1156, 1970, 3023
BspACI CCGC 5 cut(s) 29, 330, 2376, 2566, 3165
BspANI GGCC 5 cut(s) 808, 2253, 2421, 2941, 3032
BspEI TCCGGA 1 cut(s) 3214
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 2 cut(s) 241, 2209
BspLI GGNNCC 6 cut(s) 2082, 2530, 2737, 2931, 3033, 3129
BspMAI CTGCAG 2 cut(s) 886, 1798
BspQI GCTCTTC 1 cut(s) 2793
BspTI CTTAAG 1 cut(s) 119
BspTNI GGTCTC 2 cut(s) 183, 291
BsrDI GCAATG 2 cut(s) 1908, 2844
BsrFI RCCGGY 1 cut(s) 2417
BsrI ACTGG 7 cut(s) 97, 1029, 1543, 1893, 2469, 2529, 2734
BssAI RCCGGY 1 cut(s) 2417
BssECI CCNNGG 8 cut(s) 302, 421, 914, 1156, 1443, 1970, 2739, 3023
BssSI CACGAG 2 cut(s) 1740, 2091
BssT1I CCWWGG 5 cut(s) 914, 1156, 1970, 2739, 3023
Bst2BI CACGAG 2 cut(s) 1740, 2091
Bst2UI CCWGG 1 cut(s) 705
Bst6I CTCTTC 5 cut(s) 93, 762, 2793, 3024, 3331
BstAFI CTTAAG 1 cut(s) 119
BstAPI GCANNNNNTGC 1 cut(s) 2843
BstC8I GCNNGC 6 cut(s) 1439, 1798, 2289, 2295, 2483, 2784
BstDSI CCRYGG 3 cut(s) 1156, 1970, 3023
BstENI CCTNNNNNAGG 1 cut(s) 2396
BstF5I GGATG 6 cut(s) 444, 748, 1033, 2581, 2848, 3033
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 2294
BstHHI GCGC 2 cut(s) 448, 2293
BstMAI GTCTC 7 cut(s) 183, 291, 596, 1564, 1706, 2155, 2207
BstMWI GCNNNNNNNGC 3 cut(s) 1179, 1984, 2843
BstNI CCWGG 1 cut(s) 705
BstNSI RCATGY 4 cut(s) 1441, 2363, 2786, 3291
BstPAI GACNNNNGTC 1 cut(s) 3298
BstSCI CCNGG 4 cut(s) 703, 1006, 2531, 3255
BstSFI CTRYAG 2 cut(s) 882, 1794
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 8 cut(s) 217, 868, 896, 1780, 2072, 2083, 2833, 3066
BstV2I GAAGAC 5 cut(s) 662, 1411, 2330, 2343, 2517
BstX2I RGATCY 6 cut(s) 566, 748, 2127, 2528, 2929, 2959
BstXI CCANNNNNNTGG 1 cut(s) 3310
BstYI RGATCY 6 cut(s) 566, 748, 2127, 2528, 2929, 2959
BsuRI GGCC 5 cut(s) 808, 2253, 2421, 2941, 3032
BtgI CCRYGG 3 cut(s) 1156, 1970, 3023
BtgZI GCGATG 1 cut(s) 2161
BtrI CACGTC 1 cut(s) 1215
BtsCI GGATG 6 cut(s) 444, 748, 1033, 2581, 2848, 3033
BtsIMutI CAGTG 3 cut(s) 570, 1698, 2476
Cac8I GCNNGC 6 cut(s) 1439, 1798, 2289, 2295, 2483, 2784
CciI TCATGA 2 cut(s) 241, 2209
CfoI GCGC 2 cut(s) 448, 2293
Cfr10I RCCGGY 1 cut(s) 2417
Cfr13I GGNCC 3 cut(s) 1745, 3031, 3300
CseI GACGC 2 cut(s) 613, 1031
Csp6I GTAC 7 cut(s) 309, 471, 772, 1513, 1631, 1668, 1886
CviQI GTAC 7 cut(s) 309, 471, 772, 1513, 1631, 1668, 1886
DraI TTTAAA 2 cut(s) 891, 1723
DriI GACNNNNNGTC 1 cut(s) 1693
EaeI YGGCCR 2 cut(s) 806, 2419
Eam1104I CTCTTC 5 cut(s) 93, 762, 2793, 3024, 3331
Eam1105I GACNNNNNGTC 1 cut(s) 1693
EarI CTCTTC 5 cut(s) 93, 762, 2793, 3024, 3331
Eco130I CCWWGG 5 cut(s) 914, 1156, 1970, 2739, 3023
Eco24I GRGCYC 1 cut(s) 19
Eco31I GGTCTC 2 cut(s) 183, 291
Eco32I GATATC 1 cut(s) 729
Eco47I GGWCC 2 cut(s) 1745, 3300
Eco57I CTGAAG 4 cut(s) 71, 737, 1251, 2427
Eco88I CYCGRG 4 cut(s) 301, 1442, 2100, 3060
EcoNI CCTNNNNNAGG 1 cut(s) 2396
EcoO109I RGGNCCY 1 cut(s) 1745
EcoRII CCWGG 1 cut(s) 703
EcoRV GATATC 1 cut(s) 729
EcoT14I CCWWGG 5 cut(s) 914, 1156, 1970, 2739, 3023
EcoT22I ATGCAT 2 cut(s) 283, 3147
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 5 cut(s) 914, 1156, 1970, 2739, 3023
Esp3I CGTCTC 1 cut(s) 596
FalI AAGNNNNNCTT 4 cut(s) 1445, 1477, 1570, 1602
FaqI GGGAC 1 cut(s) 2548
FauI CCCGC 2 cut(s) 2559, 3158
FauNDI CATATG 3 cut(s) 283, 2539, 3091
FblI GTMKAC 2 cut(s) 1472, 3252
Fnu4HI GCNGC 8 cut(s) 231, 882, 885, 1794, 2061, 2097, 2847, 3055
FokI GGATG 6 cut(s) 451, 755, 1040, 2588, 2855, 3040
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 8 cut(s) 231, 882, 885, 1794, 2061, 2097, 2847, 3055
FspBI CTAG 7 cut(s) 390, 915, 1551, 1655, 1749, 1874, 2973
GlaI GCGC 2 cut(s) 447, 2292
GluI GCNGC 8 cut(s) 231, 882, 885, 1794, 2061, 2097, 2847, 3055
GsuI CTGGAG 5 cut(s) 1153, 1182, 2031, 2674, 2751
HaeII RGCGCY 1 cut(s) 2294
HaeIII GGCC 5 cut(s) 808, 2253, 2421, 2941, 3032
HapII CCGG 8 cut(s) 492, 1007, 1758, 2418, 2532, 3120, 3215, 3257
HgaI GACGC 2 cut(s) 613, 1031
HhaI GCGC 2 cut(s) 448, 2293
Hin6I GCGC 2 cut(s) 446, 2291
HinP1I GCGC 2 cut(s) 446, 2291
HincII GTYRAC 2 cut(s) 1273, 3253
HindII GTYRAC 2 cut(s) 1273, 3253
HindIII AAGCTT 1 cut(s) 396
HpaI GTTAAC 1 cut(s) 1273
HpaII CCGG 8 cut(s) 492, 1007, 1758, 2418, 2532, 3120, 3215, 3257
Hpy99I CGWCG 1 cut(s) 1668
HpyCH4IV ACGT 8 cut(s) 34, 78, 591, 955, 1214, 1772, 2694, 2983
HpyF10VI GCNNNNNNNGC 3 cut(s) 1179, 1984, 2843
HpySE526I ACGT 8 cut(s) 34, 78, 591, 955, 1214, 1772, 2694, 2983
HspAI GCGC 2 cut(s) 446, 2291
Kpn2I TCCGGA 1 cut(s) 3214
KspAI GTTAAC 1 cut(s) 1273
LguI GCTCTTC 1 cut(s) 2793
LmnI GCTCC 2 cut(s) 2012, 3152
Lsp1109I GCAGC 8 cut(s) 217, 868, 896, 1780, 2072, 2083, 2833, 3066
LweI GCATC 5 cut(s) 268, 1005, 1018, 2184, 2833
MaeI CTAG 7 cut(s) 390, 915, 1551, 1655, 1749, 1874, 2973
MaeII ACGT 8 cut(s) 34, 78, 591, 955, 1214, 1772, 2694, 2983
MaeIII GTNAC 6 cut(s) 430, 1348, 2749, 2772, 2984, 3319
MflI RGATCY 6 cut(s) 566, 748, 2127, 2528, 2929, 2959
MhlI GDGCHC 1 cut(s) 19
MlyI GAGTC 4 cut(s) 713, 1345, 1514, 2333
MmeI TCCRAC 2 cut(s) 504, 574
Mph1103I ATGCAT 2 cut(s) 283, 3147
MroI TCCGGA 1 cut(s) 3214
MroXI GAANNNNTTC 2 cut(s) 2435, 2648
MseI TTAA 9 cut(s) 120, 348, 690, 890, 1272, 1335, 1386, 1722, 2924
MslI CAYNNNNRTG 5 cut(s) 171, 348, 437, 1809, 3308
MspA1I CMGCKG 1 cut(s) 2376
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 8 cut(s) 492, 1007, 1758, 2418, 2532, 3120, 3215, 3257
MspR9I CCNGG 4 cut(s) 705, 1008, 2533, 3257
Mva1269I GAATGC 3 cut(s) 682, 2352, 2702
MvaI CCWGG 1 cut(s) 705
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 1179, 1984, 2843
NciI CCSGG 3 cut(s) 1008, 2533, 3257
NcoI CCATGG 3 cut(s) 1156, 1970, 3023
NdeI CATATG 3 cut(s) 283, 2539, 3091
NlaIV GGNNCC 6 cut(s) 2082, 2530, 2737, 2931, 3033, 3129
NmuCI GTSAC 4 cut(s) 430, 1348, 2772, 3319
NsiI ATGCAT 2 cut(s) 283, 3147
NspI RCATGY 4 cut(s) 1441, 2363, 2786, 3291
PaeI GCATGC 2 cut(s) 1441, 2786
PagI TCATGA 2 cut(s) 241, 2209
PciSI GCTCTTC 1 cut(s) 2793
PcsI WCGNNNNNNNCGW 1 cut(s) 2098
PctI GAATGC 3 cut(s) 682, 2352, 2702
PdmI GAANNNNTTC 2 cut(s) 2435, 2648
PfoI TCCNGGA 2 cut(s) 703, 2531
PkrI GCNGC 8 cut(s) 232, 883, 886, 1795, 2062, 2098, 2848, 3056
PleI GAGTC 4 cut(s) 713, 1345, 1514, 2333
PpsI GAGTC 4 cut(s) 713, 1345, 1514, 2333
PpuMI RGGWCCY 1 cut(s) 1745
PshAI GACNNNNGTC 1 cut(s) 3298
PsiI TTATAA 1 cut(s) 2135
Psp1406I AACGTT 2 cut(s) 78, 2694
Psp5II RGGWCCY 1 cut(s) 1745
Psp6I CCWGG 1 cut(s) 703
PspGI CCWGG 1 cut(s) 703
PspN4I GGNNCC 6 cut(s) 2082, 2530, 2737, 2931, 3033, 3129
PspPI GGNCC 3 cut(s) 1745, 3031, 3300
PspPPI RGGWCCY 1 cut(s) 1745
PstI CTGCAG 2 cut(s) 886, 1798
PsuI RGATCY 6 cut(s) 566, 748, 2127, 2528, 2929, 2959
RsaI GTAC 7 cut(s) 310, 472, 773, 1514, 1632, 1669, 1887
RsaNI GTAC 7 cut(s) 309, 471, 772, 1513, 1631, 1668, 1886
RseI CAYNNNNRTG 5 cut(s) 171, 348, 437, 1809, 3308
SalI GTCGAC 1 cut(s) 3251
SapI GCTCTTC 1 cut(s) 2793
SaqAI TTAA 9 cut(s) 120, 348, 690, 890, 1272, 1335, 1386, 1722, 2924
SatI GCNGC 8 cut(s) 231, 882, 885, 1794, 2061, 2097, 2847, 3055
Sau96I GGNCC 3 cut(s) 1745, 3031, 3300
ScaI AGTACT 2 cut(s) 310, 1887
SchI GAGTC 4 cut(s) 713, 1345, 1514, 2333
ScrFI CCNGG 4 cut(s) 705, 1008, 2533, 3257
SduI GDGCHC 1 cut(s) 19
SfaNI GCATC 5 cut(s) 268, 1005, 1018, 2184, 2833
SfcI CTRYAG 2 cut(s) 882, 1794
SinI GGWCC 2 cut(s) 1745, 3300
SmiMI CAYNNNNRTG 5 cut(s) 171, 348, 437, 1809, 3308
SmlI CTYRAG 3 cut(s) 119, 320, 647
SmoI CTYRAG 3 cut(s) 119, 320, 647
SphI GCATGC 2 cut(s) 1441, 2786
SsiI CCGC 5 cut(s) 29, 330, 2376, 2566, 3165
SspI AATATT 1 cut(s) 2388
SspMI CTAG 7 cut(s) 390, 915, 1551, 1655, 1749, 1874, 2973
StyD4I CCNGG 4 cut(s) 703, 1006, 2531, 3255
StyI CCWWGG 5 cut(s) 914, 1156, 1970, 2739, 3023
TaiI ACGT 8 cut(s) 37, 81, 594, 958, 1217, 1775, 2697, 2986
TaqI TCGA 9 cut(s) 19, 295, 617, 1021, 1224, 1686, 2873, 3187, 3252
TaqII GACCGA 1 cut(s) 3288
TatI WGTACW 4 cut(s) 308, 771, 1630, 1885
Tru1I TTAA 9 cut(s) 120, 348, 690, 890, 1272, 1335, 1386, 1722, 2924
Tru9I TTAA 9 cut(s) 120, 348, 690, 890, 1272, 1335, 1386, 1722, 2924
TscAI CASTG 3 cut(s) 577, 1705, 2476
TseFI GTSAC 4 cut(s) 430, 1348, 2772, 3319
TseI GCWGC 8 cut(s) 230, 881, 884, 1793, 2060, 2096, 2846, 3054
Tsp45I GTSAC 4 cut(s) 430, 1348, 2772, 3319
TspGWI ACGGA 4 cut(s) 1425, 2588, 2611, 3063
TspRI CASTG 3 cut(s) 577, 1705, 2476
Vha464I CTTAAG 1 cut(s) 119
VpaK11BI GGWCC 2 cut(s) 1745, 3300
XagI CCTNNNNNAGG 1 cut(s) 2396
XapI RAATTY 4 cut(s) 412, 1724, 1815, 2161
XbaI TCTAGA 1 cut(s) 2972
XceI RCATGY 4 cut(s) 1441, 2363, 2786, 3291
XmaJI CCTAGG 1 cut(s) 914
XmiI GTMKAC 2 cut(s) 1472, 3252
XmnI GAANNNNTTC 2 cut(s) 2435, 2648
XspI CTAG 7 cut(s) 390, 915, 1551, 1655, 1749, 1874, 2973
ZrmI AGTACT 2 cut(s) 310, 1887
Zsp2I ATGCAT 2 cut(s) 283, 3147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.