pycom05g16570

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009051
Physical Location & Seq
Reverse (-)
19850690 .. 19856461
5772 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16570.8

Sequence Viewer

Length: 3825 bp
ATGCAGCTAAGTATGTCCAAGCACTTGTTTGATACCATGACCAATGAGGTGCCTGGCACATCAGGTCAATGGATCCAGAAAGAAGCGACTTTTGTTGTCCCAAACACAGTAAAGAATGTACATAAGAATGTTTCTGTACAGACAGGTGAGGAATTTTCTATGGAGTTTCTTCAGGATCGTTCTGCAGCGAGAAGAGTTCCTGCTGTGACTGATATGGTTGAGAATCGCGAAAATGGGGTTGGGTTGAATTATAATCAGAATAATCGGCTGGGATATCAGGATCTTACTGATCTTCTGGGGTTGAGGAGAATGGATTCCGAGTGTGCATCTGATACGTCTGACTTTATCTCGGCAAAAGGGTCCAGTAAAGATACTGAAGGCGAGGCTTGTCTTGACAAGTTAGGCAGATGCAACAGGGAGGAGGTTGATAGCGGACAAGGATCAAGGAAGGCTTTTGGAGAACTGAATGTTGACAGAGCTGGGTTTGGACCAACTGCCTTACCTATGTATATGTCTGAGTCCCATCATTCCAACACTGTTAATGGTTCAGGAGCATTGGATGGTTCTCAATCTGGGAAGATGAAGTTTCTTTGCAGTTTTGGTGGAAAAATTTTGCCCAGGCCTAGCGATGGGAAACTCAGATATGTTGGGGGGGAGACGCGCATTATTTCCTTCAGGAAAAGTATTTCATGGGAAGAGCTTGTGAAGAAAACTTTCAGTTTTTGCAACCAACCTCATACAATCAAGTACCAGCTCCCAAGCGAGGATCTTGATGCTCTTATATCCGTTTCTTCTGATGAAGATCTTCAAAATATGATAGAGGAGTACCATGGCCTCGAAAGGCATGAGGGTTCTCAGAGACCTCGAATCTTTTTGATTCCGTTAGGTGAATCTGAAAACACATCTTCCTTTGAGGCAGATAGCATACATCAAAGTAACCCTGATTACCAATATGTTGCAGCTGTAAATGGCATGATCGATCCTAGTCCTAGGAAAAACATTGGAGGTAAGAATTCGACTACTGAAGCAAGTCAACAGGGAACCAAGACTGTCCTATTCCCTATGGAAATTAGGAGTGATTTTAAAGCTTTGCATCCTAATCAAAATTTAAGTGAACCCCAGGATATGACTCGGTCTCCCATTCAGTCTTCCTTTTCTCCCATTCTGCATCAGCAAGGAGATTCCAAGGGTGTTCATTTACAATCACATGGTCTAAACTCATGCCAGGGCAGTAATGAGAGTAGCAGTTCTTTTATAAGTGCTCAACCACCACAAGATAACTCCAGCAATAGCACGGAAGGTTATAAAATTCATCCACAGGGGGCAGTAACTCTTATGGATTATCACCACCCTTGTAAGCAAGCTGATGATGGACAACTGGGCCTATACCATGGAGGACATAGTCTCAACCACAATCCCAGCAAAGATCCCATATCTACTTTAGTTGTCGGTCAAAATGTTGGTGATTTTGATGGATTCTCCCATGAGAGGCCAGTGCAGAAGGAAAGAATATTCTCTCCTGAGCTTGTCTCACACCAAGAAGATTCAAAGAATATGTTGTCAGGATCTAATGATTACGTTGATTGTCATCCTGGCATGCATCATGCATATTCTGATTCAAAACTGCAAGAGAATGGAAGGAGGTCTGTATATTGTTCACAAGAAGGAATAAGCCCACCGTCCCCCTTAATTTTTGCAAAGGCTCAATCATCATTGCTGCTAAACTCAGTTATTTCTCAAGAAAAGCCGACTCTACTGCGTGACAATATTGAATCTTTGAATCCTAGGTTACACAATCAGTTACATGGTACGGAGTCGATAGGATTGCGCAGCAGACTGGATTTGCCGAATTCATCTCCATGTTTAGAATCACTGGGCAGGAATGAAGATTCTCCCAAATGTAATGATTTTCATGAAAAATGCCGGACAGCTAAACAGAAAGATTCTTTGACTTTGGAGCAGACGAAGAAGGTCAATCAAAAGGATCCTTTCTTACATCAAGATGAAACCCTTTATGGGACAAGATCACCTGCCACTGAAGTTGATTATCGGAATGGATTTCCTAATATAATCCCCGAACCATCATCTACTTTTGCCGCTGGGGTTGTTCCTGCAGCAATTAATTTACAACCGTTGGTAAACAAGAAGGTGGAGGATTCACAAAGCTTCCAGGGTGACAAAAGCCCTGCCGATCTTCTTGTCACGAGTCAAAGAACTGCCAATGATCAGGACTGTGCTTTGGCAGTGATGCCTAGTGGTGAAAAAGGACATGATGTTTCAGGGGCCAGGAATTCTGAAGTTGCAGGCATATTTCCAAGTACTAAACAGCATTCTCGTGATGAAAATTCTTTGGCTGATCTCATCTCAGGGTTGTCCAATGGCCAAGTTTCCCATGAGCCTGCACGACCAGAACTTGTTGCAAGTCAAAAGGATATGCGTTTCCAAGAACCCCTGCATATGAACTCTGCACATGTGCACCCAGTGACAGTTCTTCATGATCCTGTACTGGAAAAGAGTGACCACATGGTGTTGCATAAGCCAGTCCAAGATGTTGCGTTTAAAAGACAGGTCTCTCTTCTTGATGATGACTTTGTAAATTACCCTGATAAGAATGCTGAGAAGTTGAGCTCAAATGTCGAAGATGTTAGTTTGGCACCAACAAAACCCCCAACAATGAGGAATGATAAAAAACAGTTGGAGTCAGTGAAAATTGTGGAAGACATAACGAACGGTATTATTTCTGGCATTCAGTCTTCATCACCAGTTTCCCCATATGCTGTGGACGAACCTGTTGTTGATCTCATATCTCCTACTGCAACAGAGGTGGAGAGCGTCAATGAAGAGTCTGAGTATGAGGATGATAAGGCTGATGAAGAAGACAAAAATGAATCATTTAGTGATGCAATGATTGCTGAAATGGAAGCCAGCATCTATGGCTTGCAGATTATAAAAAATGCTGATCTTGAAGAATTAAGGGAATTAGGATCTGGTACATATGGAACTGTTTATCATGGAAAATGGCGGGGAACAGATGTTGCTATAAAGCGAATAAAAAAGAGCTGTTTTGCAGGAAGATCATCAGAGCAAGACCGACTGACAAAAGACTTCTGGAGAGAGGCGCAAATCCTTTCAGCTCTCCATCATCCAAATGTGGTTGCATTCTATGGAGTAGTACCTGATGGAGCTGGAGGAACGTTGGCAACTGTAACTGAGTACATGGTGAATGGATCACTGAGGCATGCCCTACTAAAAAAGGATAGATCACTTGACCGTCGTAGAAAGCTTATAATTGCCATGGATGCAGCTTTTGGCATGGAATACTTGCATTCAAAGAATATTGTCCATTTTGATTTAAAGTGTGACAATCTGCTAGTCAACTTGAGGGATCCTCAACGACCTATATGCAAGGTTGGGGATTTTGGTTTATCGAGAATCAAACGCAATACCTTGGTATCTGGTGGTGTGCGAGGAACTCTTCCTTGGATGGCACCAGAACTATTAAATGGCAGCAGCATCCGGGTTTCCGAGAAGGTTGATGTTTTCTCATTTGGAATTTCAATGTGGGAGATCTTGACCGGGGAGGAACCATATGCCAATATGCATTGCGGTGCCATCATTGGTGGAATTGTGAAGAACACTCTTCGACCTCCTATTCCAGAACGTTGTGATTCTGAGTGGAAGAACCTGATGGAACAGTGCTGGTCACCTGATCCTGACATCCGTCCATCATTCACCGAGATAACTAACAGGTTGCGAGCTATGTCCAACGCACTTCGGGCGAAGGTACCTAGCAATCAGACAAACCAGATAAAGCCCAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1275

Amino Acids

140.89

Weight (kDa)

5.61

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 252, 1256, 1305, 2948, 3287
AarI CACCTGC 1 cut(s) 2038
Acc16I TGCGCA 1 cut(s) 1829
Acc36I ACCTGC 1 cut(s) 2038
Acc65I GGTACC 1 cut(s) 3784
AccB1I GGYRCC 5 cut(s) 49, 2653, 3487, 3608, 3784
AccII CGCG 2 cut(s) 228, 661
AciI CCGC 4 cut(s) 432, 2097, 3022, 3606
AclI AACGTT 2 cut(s) 3194, 3661
AcoI YGGCCR 1 cut(s) 2380
AcsI RAATTY 9 cut(s) 152, 609, 1012, 1105, 1308, 1849, 2290, 2344, 3552
AcuI CTGAAG 6 cut(s) 155, 396, 658, 1044, 2058, 2316
AdeI CACNNNGTG 2 cut(s) 2482, 2527
AfiI CCNNNNNNNGG 4 cut(s) 629, 763, 1488, 2016
AflIII ACRYGT 1 cut(s) 2470
AgsI TTSAA 9 cut(s) 247, 809, 1548, 1620, 1772, 1780, 2966, 3330, 3558
AjnI CCWGG 7 cut(s) 52, 617, 1119, 1224, 1591, 2169, 2285
AjuI GAANNNNNNNTTGG 4 cut(s) 222, 254, 3463, 3495
Alw21I GWGCWC 3 cut(s) 1264, 2478, 2630
Alw26I GTCTC 6 cut(s) 650, 853, 1140, 1409, 1534, 2575
Alw44I GTGCAC 1 cut(s) 2474
AoxI GGCC 6 cut(s) 620, 832, 1381, 1490, 2283, 2380
ApaLI GTGCAC 1 cut(s) 2474
ApeKI GCWGC 9 cut(s) 4, 185, 959, 1717, 1830, 2114, 3302, 3507, 3510
ApoI RAATTY 9 cut(s) 152, 609, 1012, 1105, 1308, 1849, 2290, 2344, 3552
AseI ATTAAT 1 cut(s) 2121
Asp700I GAANNNNTTC 3 cut(s) 313, 713, 804
Asp718I GGTACC 1 cut(s) 3784
AspA2I CCTAGG 2 cut(s) 989, 1784
AspLEI GCGC 3 cut(s) 663, 1830, 3121
AspS9I GGNCC 4 cut(s) 360, 488, 1381, 2283
AsuC2I CCSGG 2 cut(s) 3518, 3577
AvaII GGWCC 2 cut(s) 360, 488
AvrII CCTAGG 2 cut(s) 989, 1784
BaeGI GKGCMC 1 cut(s) 2478
BaeI ACNNNNGTAYC 2 cut(s) 3435, 3468
BalI TGGCCA 1 cut(s) 2382
BamHI GGATCC 3 cut(s) 72, 1984, 3385
BanI GGYRCC 5 cut(s) 49, 2653, 3487, 3608, 3784
BanII GRGCYC 1 cut(s) 2630
BauI CACGAG 2 cut(s) 2203, 2334
BbsI GAAGAC 4 cut(s) 1140, 2724, 2745, 2883
Bbv12I GWGCWC 3 cut(s) 1264, 2478, 2630
BbvI GCAGC 9 cut(s) 16, 197, 971, 1704, 1842, 2126, 3314, 3519, 3522
BcgI CGANNNNNNTGC 6 cut(s) 1738, 1772, 1807, 1841, 3384, 3418
BciT130I CCWGG 7 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287
BclI TGATCA 1 cut(s) 2224
BcnI CCSGG 2 cut(s) 3518, 3577
BcoDI GTCTC 6 cut(s) 650, 853, 1140, 1409, 1534, 2575
BfaI CTAG 7 cut(s) 624, 984, 990, 1785, 2253, 3371, 3789
BfmI CTRYAG 2 cut(s) 183, 2112
BfuAI ACCTGC 1 cut(s) 2038
BglII AGATCT 2 cut(s) 802, 3567
BlnI CCTAGG 2 cut(s) 989, 1784
BmcAI AGTACT 1 cut(s) 2320
Bme1390I CCNGG 9 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287, 3518, 3577
Bme18I GGWCC 2 cut(s) 360, 488
BmgT120I GGNCC 4 cut(s) 360, 488, 1381, 2283
BmrFI CCNGG 9 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287, 3518, 3577
BmrI ACTGGG 3 cut(s) 1388, 1883, 2474
BmuI ACTGGG 3 cut(s) 1388, 1883, 2474
BoxI GACNNNNGTC 1 cut(s) 3720
BpiI GAAGAC 4 cut(s) 1140, 2724, 2745, 2883
BplI GAGNNNNNCTC 4 cut(s) 1514, 1546, 3373, 3405
BpmI CTGGAG 3 cut(s) 1267, 3130, 3207
Bpu10I CCTNAGC 1 cut(s) 1521
BpuEI CTTGAG 2 cut(s) 1722, 3400
BpuMI CCSGG 2 cut(s) 3518, 3577
Bsa29I ATCGAT 1 cut(s) 978
BsaBI GATNNNNATC 2 cut(s) 801, 1587
BsaI GGTCTC 3 cut(s) 853, 1140, 2575
BsaXI ACNNNNNCTCC 2 cut(s) 1120, 1150
Bsc4I CCNNNNNNNGG 4 cut(s) 629, 763, 1488, 2016
Bse1I ACTGG 9 cut(s) 363, 1383, 1493, 1842, 1878, 2480, 2511, 2540, 2762
Bse3DI GCAATG 3 cut(s) 1712, 2910, 3601
Bse8I GATNNNNATC 2 cut(s) 801, 1587
BseBI CCWGG 7 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287
BseCI ATCGAT 1 cut(s) 978
BseJI GATNNNNATC 2 cut(s) 801, 1587
BseLI CCNNNNNNNGG 4 cut(s) 629, 763, 1488, 2016
BseMI GCAATG 3 cut(s) 1712, 2910, 3601
BseNI ACTGG 9 cut(s) 363, 1383, 1493, 1842, 1878, 2480, 2511, 2540, 2762
BseRI GAGGAG 3 cut(s) 319, 434, 836
BseSI GKGCMC 1 cut(s) 2478
BseXI GCAGC 9 cut(s) 16, 197, 971, 1704, 1842, 2126, 3314, 3519, 3522
BseYI CCCAGC 4 cut(s) 268, 479, 1418, 2099
BsgI GTGCAG 3 cut(s) 1517, 2385, 2451
Bsh1236I CGCG 2 cut(s) 228, 661
BshFI GGCC 6 cut(s) 622, 834, 1383, 1492, 2285, 2382
BshNI GGYRCC 5 cut(s) 49, 2653, 3487, 3608, 3784
BshVI ATCGAT 1 cut(s) 978
BsiHKAI GWGCWC 3 cut(s) 1264, 2478, 2630
BsiSI CCGG 3 cut(s) 1924, 3517, 3576
BslFI GGGAC 4 cut(s) 83, 505, 1666, 2032
BslI CCNNNNNNNGG 4 cut(s) 629, 763, 1488, 2016
BsmAI GTCTC 6 cut(s) 650, 853, 1140, 1409, 1534, 2575
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 4 cut(s) 83, 505, 1666, 2032
BsmI GAATGC 5 cut(s) 2329, 2617, 2745, 3158, 3325
BsnI GGCC 6 cut(s) 622, 834, 1383, 1492, 2285, 2382
Bso31I GGTCTC 3 cut(s) 853, 1140, 2575
Bsp1286I GDGCHC 3 cut(s) 1264, 2478, 2630
Bsp1407I TGTACA 2 cut(s) 118, 136
Bsp19I CCATGG 3 cut(s) 829, 1390, 3294
Bsp68I TCGCGA 1 cut(s) 228
BspACI CCGC 4 cut(s) 432, 2097, 3022, 3606
BspANI GGCC 6 cut(s) 622, 834, 1383, 1492, 2285, 2382
BspDI ATCGAT 1 cut(s) 978
BspFNI CGCG 2 cut(s) 228, 661
BspHI TCATGA 2 cut(s) 1912, 2494
BspMAI CTGCAG 2 cut(s) 187, 2116
BspMI ACCTGC 1 cut(s) 2038
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 5 cut(s) 49, 2653, 3487, 3608, 3784
BspTNI GGTCTC 3 cut(s) 853, 1140, 2575
BsrDI GCAATG 3 cut(s) 1712, 2910, 3601
BsrGI TGTACA 2 cut(s) 118, 136
BsrI ACTGG 9 cut(s) 363, 1383, 1493, 1842, 1878, 2480, 2511, 2540, 2762
BssSI CACGAG 2 cut(s) 2203, 2334
BssT1I CCWWGG 8 cut(s) 829, 989, 1185, 1390, 1784, 3294, 3447, 3479
Bst2BI CACGAG 2 cut(s) 2203, 2334
Bst2UI CCWGG 7 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287
Bst6I CTCTTC 6 cut(s) 187, 690, 2580, 2835, 3480, 3645
BstAPI GCANNNNNTGC 1 cut(s) 2909
BstAUI TGTACA 2 cut(s) 118, 136
BstC8I GCNNGC 8 cut(s) 1362, 1598, 2305, 2400, 2926, 2939, 3240, 3756
BstDSI CCRYGG 3 cut(s) 829, 1390, 3294
BstEII GGTNACC 1 cut(s) 3702
BstFNI CGCG 2 cut(s) 228, 661
BstHHI GCGC 3 cut(s) 663, 1830, 3121
BstMAI GTCTC 6 cut(s) 650, 853, 1140, 1409, 1534, 2575
BstMWI GCNNNNNNNGC 4 cut(s) 2909, 2934, 3299, 3776
BstNI CCWGG 7 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287
BstNSI RCATGY 3 cut(s) 1600, 2474, 3242
BstPAI GACNNNNGTC 1 cut(s) 3720
BstPI GGTNACC 1 cut(s) 3702
BstSCI CCNGG 9 cut(s) 52, 617, 1119, 1224, 1591, 2169, 2285, 3516, 3575
BstSFI CTRYAG 2 cut(s) 183, 2112
BstSLI GKGCMC 1 cut(s) 2478
BstUI CGCG 2 cut(s) 228, 661
BstV1I GCAGC 9 cut(s) 16, 197, 971, 1704, 1842, 2126, 3314, 3519, 3522
BstV2I GAAGAC 4 cut(s) 1140, 2724, 2745, 2883
Bsu15I ATCGAT 1 cut(s) 978
BsuRI GGCC 6 cut(s) 622, 834, 1383, 1492, 2285, 2382
BsuTUI ATCGAT 1 cut(s) 978
BtgI CCRYGG 3 cut(s) 829, 1390, 3294
BtgZI GCGATG 1 cut(s) 642
BtsI GCAGTG 1 cut(s) 2250
BtsIMutI CAGTG 9 cut(s) 534, 1500, 1871, 2034, 2250, 2487, 2709, 3230, 3701
BtuMI TCGCGA 1 cut(s) 228
BveI ACCTGC 1 cut(s) 2038
Cac8I GCNNGC 8 cut(s) 1362, 1598, 2305, 2400, 2926, 2939, 3240, 3756
CciI TCATGA 2 cut(s) 1912, 2494
CfoI GCGC 3 cut(s) 663, 1830, 3121
Cfr13I GGNCC 4 cut(s) 360, 488, 1381, 2283
ClaI ATCGAT 1 cut(s) 978
CseI GACGC 2 cut(s) 667, 2821
CspCI CAANNNNNGTGG 4 cut(s) 2023, 2058, 2805, 2840
DraI TTTAAA 3 cut(s) 1084, 2560, 3354
DraIII CACNNNGTG 2 cut(s) 2482, 2527
EaeI YGGCCR 1 cut(s) 2380
Eam1104I CTCTTC 6 cut(s) 187, 690, 2580, 2835, 3480, 3645
EarI CTCTTC 6 cut(s) 187, 690, 2580, 2835, 3480, 3645
Ecl136II GAGCTC 1 cut(s) 2628
Eco130I CCWWGG 8 cut(s) 829, 989, 1185, 1390, 1784, 3294, 3447, 3479
Eco147I AGGCCT 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 2630
Eco31I GGTCTC 3 cut(s) 853, 1140, 2575
Eco32I GATATC 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 360, 488
Eco53kI GAGCTC 1 cut(s) 2628
Eco57I CTGAAG 6 cut(s) 155, 396, 658, 1044, 2058, 2316
Eco91I GGTNACC 1 cut(s) 3702
EcoICRI GAGCTC 1 cut(s) 2628
EcoO65I GGTNACC 1 cut(s) 3702
EcoRI GAATTC 3 cut(s) 1012, 1849, 2290
EcoRII CCWGG 7 cut(s) 52, 617, 1119, 1224, 1591, 2169, 2285
EcoRV GATATC 1 cut(s) 275
EcoT14I CCWWGG 8 cut(s) 829, 989, 1185, 1390, 1784, 3294, 3447, 3479
EcoT22I ATGCAT 3 cut(s) 1602, 1609, 3603
EcoT38I GRGCYC 1 cut(s) 2630
ErhI CCWWGG 8 cut(s) 829, 989, 1185, 1390, 1784, 3294, 3447, 3479
Esp3I CGTCTC 1 cut(s) 650
FalI AAGNNNNNCTT 2 cut(s) 436, 468
FaqI GGGAC 4 cut(s) 83, 505, 1666, 2032
FauI CCCGC 1 cut(s) 3015
FauNDI CATATG 4 cut(s) 2457, 2773, 2995, 3589
FbaI TGATCA 1 cut(s) 2224
FriOI GRGCYC 1 cut(s) 2630
FspBI CTAG 7 cut(s) 624, 984, 990, 1785, 2253, 3371, 3789
FspI TGCGCA 1 cut(s) 1829
GlaI GCGC 3 cut(s) 662, 1829, 3120
GsaI CCCAGC 4 cut(s) 272, 483, 1422, 2103
GsuI CTGGAG 3 cut(s) 1267, 3130, 3207
HaeIII GGCC 6 cut(s) 622, 834, 1383, 1492, 2285, 2382
HapII CCGG 3 cut(s) 1924, 3517, 3576
HgaI GACGC 2 cut(s) 667, 2821
HhaI GCGC 3 cut(s) 663, 1830, 3121
Hin6I GCGC 3 cut(s) 661, 1828, 3119
HinP1I GCGC 3 cut(s) 661, 1828, 3119
HincII GTYRAC 3 cut(s) 472, 1034, 3376
HindII GTYRAC 3 cut(s) 472, 1034, 3376
HindIII AAGCTT 3 cut(s) 1086, 2164, 3281
HpaII CCGG 3 cut(s) 1924, 3517, 3576
Hpy166II GTNNAC 8 cut(s) 472, 1034, 1115, 1658, 2140, 2476, 2782, 3376
Hpy8I GTNNAC 8 cut(s) 472, 1034, 1115, 1658, 2140, 2476, 2782, 3376
Hpy99I CGWCG 1 cut(s) 3276
HpyCH4IV ACGT 4 cut(s) 335, 1578, 3194, 3661
HpyF10VI GCNNNNNNNGC 4 cut(s) 2909, 2934, 3299, 3776
HpySE526I ACGT 4 cut(s) 335, 1578, 3194, 3661
HspAI GCGC 3 cut(s) 661, 1828, 3119
KpnI GGTACC 1 cut(s) 3788
Ksp22I TGATCA 1 cut(s) 2224
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 4 cut(s) 551, 759, 1957, 3182
Lsp1109I GCAGC 9 cut(s) 16, 197, 971, 1704, 1842, 2126, 3314, 3519, 3522
MaeI CTAG 7 cut(s) 624, 984, 990, 1785, 2253, 3371, 3789
MaeII ACGT 4 cut(s) 335, 1578, 3194, 3661
MhlI GDGCHC 3 cut(s) 1264, 2478, 2630
MlsI TGGCCA 1 cut(s) 2382
MluNI TGGCCA 1 cut(s) 2382
MlyI GAGTC 7 cut(s) 527, 1123, 1744, 1823, 2215, 2708, 2852
MmeI TCCRAC 3 cut(s) 555, 2676, 3789
Mox20I TGGCCA 1 cut(s) 2382
Mph1103I ATGCAT 3 cut(s) 1602, 1609, 3603
MroXI GAANNNNTTC 3 cut(s) 313, 713, 804
MscI TGGCCA 1 cut(s) 2382
MseI TTAA 9 cut(s) 540, 1083, 1109, 1688, 2121, 2559, 2972, 3353, 3500
MslI CAYNNNNRTG 6 cut(s) 126, 1858, 2001, 2334, 3147, 3606
Msp20I TGGCCA 1 cut(s) 2382
MspA1I CMGCKG 2 cut(s) 962, 2099
MspI CCGG 3 cut(s) 1924, 3517, 3576
MspR9I CCNGG 9 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287, 3518, 3577
Mva1269I GAATGC 5 cut(s) 2329, 2617, 2745, 3158, 3325
MvaI CCWGG 7 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287
MvnI CGCG 2 cut(s) 228, 661
MwoI GCNNNNNNNGC 4 cut(s) 2909, 2934, 3299, 3776
NciI CCSGG 2 cut(s) 3518, 3577
NcoI CCATGG 3 cut(s) 829, 1390, 3294
NdeI CATATG 4 cut(s) 2457, 2773, 2995, 3589
NmeAIII GCCGAG 1 cut(s) 329
NmuCI GTSAC 8 cut(s) 205, 1760, 2174, 2200, 2482, 2516, 3359, 3702
NruI TCGCGA 1 cut(s) 228
NsbI TGCGCA 1 cut(s) 1829
NsiI ATGCAT 3 cut(s) 1602, 1609, 3603
NspI RCATGY 3 cut(s) 1600, 2474, 3242
PaeI GCATGC 2 cut(s) 1600, 3242
PagI TCATGA 2 cut(s) 1912, 2494
PaqCI CACCTGC 1 cut(s) 2038
PceI AGGCCT 1 cut(s) 622
PciI ACATGT 1 cut(s) 2470
PciSI GCTCTTC 1 cut(s) 690
PctI GAATGC 5 cut(s) 2329, 2617, 2745, 3158, 3325
PdmI GAANNNNTTC 3 cut(s) 313, 713, 804
PflFI GACNNNGTC 2 cut(s) 1132, 1401
PleI GAGTC 7 cut(s) 526, 1123, 1744, 1822, 2214, 2707, 2851
PpsI GAGTC 7 cut(s) 526, 1123, 1744, 1822, 2214, 2707, 2851
PscI ACATGT 1 cut(s) 2470
PshAI GACNNNNGTC 1 cut(s) 3720
PshBI ATTAAT 1 cut(s) 2121
PsiI TTATAA 5 cut(s) 252, 1256, 1305, 2948, 3287
Psp124BI GAGCTC 1 cut(s) 2630
Psp1406I AACGTT 2 cut(s) 3194, 3661
Psp6I CCWGG 7 cut(s) 52, 617, 1119, 1224, 1591, 2169, 2285
PspEI GGTNACC 1 cut(s) 3702
PspFI CCCAGC 4 cut(s) 268, 479, 1418, 2099
PspGI CCWGG 7 cut(s) 52, 617, 1119, 1224, 1591, 2169, 2285
PspPI GGNCC 4 cut(s) 360, 488, 1381, 2283
PstI CTGCAG 2 cut(s) 187, 2116
PsyI GACNNNGTC 2 cut(s) 1132, 1401
PvuII CAGCTG 1 cut(s) 962
RruI TCGCGA 1 cut(s) 228
RseI CAYNNNNRTG 6 cut(s) 126, 1858, 2001, 2334, 3147, 3606
SacI GAGCTC 1 cut(s) 2630
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 9 cut(s) 540, 1083, 1109, 1688, 2121, 2559, 2972, 3353, 3500
Sau96I GGNCC 4 cut(s) 360, 488, 1381, 2283
ScaI AGTACT 1 cut(s) 2320
SchI GAGTC 7 cut(s) 527, 1123, 1744, 1823, 2215, 2708, 2852
ScrFI CCNGG 9 cut(s) 54, 619, 1121, 1226, 1593, 2171, 2287, 3518, 3577
SduI GDGCHC 3 cut(s) 1264, 2478, 2630
SfcI CTRYAG 2 cut(s) 183, 2112
SinI GGWCC 2 cut(s) 360, 488
SmiMI CAYNNNNRTG 6 cut(s) 126, 1858, 2001, 2334, 3147, 3606
SmlI CTYRAG 2 cut(s) 1737, 3379
SmoI CTYRAG 2 cut(s) 1737, 3379
SphI GCATGC 2 cut(s) 1600, 3242
SseBI AGGCCT 1 cut(s) 622
SsiI CCGC 4 cut(s) 432, 2097, 3022, 3606
SspI AATATT 3 cut(s) 1512, 1768, 3337
SspMI CTAG 7 cut(s) 624, 984, 990, 1785, 2253, 3371, 3789
SstI GAGCTC 1 cut(s) 2630
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 9 cut(s) 52, 617, 1119, 1224, 1591, 2169, 2285, 3516, 3575
StyI CCWWGG 8 cut(s) 829, 989, 1185, 1390, 1784, 3294, 3447, 3479
TaiI ACGT 4 cut(s) 338, 1581, 3197, 3664
TaqI TCGA 8 cut(s) 837, 865, 978, 1016, 1817, 2637, 3428, 3643
TaqII GACCGA 3 cut(s) 1122, 1439, 3105
TatI WGTACW 5 cut(s) 118, 136, 2318, 2503, 3213
TauI GCSGC 1 cut(s) 2099
Tru1I TTAA 9 cut(s) 540, 1083, 1109, 1688, 2121, 2559, 2972, 3353, 3500
Tru9I TTAA 9 cut(s) 540, 1083, 1109, 1688, 2121, 2559, 2972, 3353, 3500
TscAI CASTG 9 cut(s) 541, 1500, 1878, 2041, 2250, 2487, 2709, 3237, 3701
TseFI GTSAC 8 cut(s) 205, 1760, 2174, 2200, 2482, 2516, 3359, 3702
TseI GCWGC 9 cut(s) 4, 185, 959, 1717, 1830, 2114, 3302, 3507, 3510
Tsp45I GTSAC 8 cut(s) 205, 1760, 2174, 2200, 2482, 2516, 3359, 3702
TspGWI ACGGA 5 cut(s) 775, 870, 1310, 1826, 3710
TspRI CASTG 9 cut(s) 541, 1500, 1878, 2041, 2250, 2487, 2709, 3237, 3701
Tth111I GACNNNGTC 2 cut(s) 1132, 1401
VneI GTGCAC 1 cut(s) 2474
VpaK11BI GGWCC 2 cut(s) 360, 488
VspI ATTAAT 1 cut(s) 2121
XapI RAATTY 9 cut(s) 152, 609, 1012, 1105, 1308, 1849, 2290, 2344, 3552
XceI RCATGY 3 cut(s) 1600, 2474, 3242
XmaJI CCTAGG 2 cut(s) 989, 1784
XmnI GAANNNNTTC 3 cut(s) 313, 713, 804
XspI CTAG 7 cut(s) 624, 984, 990, 1785, 2253, 3371, 3789
ZrmI AGTACT 1 cut(s) 2320
Zsp2I ATGCAT 3 cut(s) 1602, 1609, 3603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.