MD05G1179200.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009051
Physical Location & Seq
Reverse (-)
30686161 .. 30693014
6854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1179200.v1.1.491

Sequence Viewer

Length: 3864 bp
ATGACAGCTTCCCAGCTCCCCAAACCCAGCTGCAACACACAGCTAAGTAGGTCCGAGCACTTGCTTGATACCATGACCAATGAGGTGCCTGGCACATCAGGTCAATGGATCCAGAAAGAAGCGACGTTTGTTGTCCCAAACACAGTAAAGAATGTACATAAGAATGTCTCTGTACAGACAGGTGAGGAATTTTCCATGGAGTTTCTTCAGGATCGTTCTGCAGTTAGAAGAGTTCCTGTTGTGACTGATATGGTTGAGAATCGTGAAAATGGGGTTGGGTTGAATTATAATCAGAATAATCGGCTGGGATATCAGGATCTTACTGATCTTCTGGGGTTGAGGAGAATGGATTCTGAGTGTGCATCTGATATGTCTGACTTTATCTCAGCAAAAGGGTCCTGTAAAGATATTGAAAGCGAGGCTTGTCTTGACAAGTTAGGCAGATGCAACAGGGAGGAGGTTGATAGCGGACAAGGATCAAGGAAGGCTTTTGGAGAACTGAATGTCGACAGAGCTGGGTTTGGACCAACTGCCTTACCTATATATATGTCTGAGTCCCATCATTCCAACACTGTTAATGGGTCAGGAGCATTGGATGGTTCTCAATCTGGGAAGATGAAGTTTCTTTGCAGTTTTGGTGGAAAAATTTTGCCCAGGCCTAGCGATGGGAAACTCAGATATGTTGGGGGAGAGACGCGCATTATTTCCTTCAGGAAAAGTATTTCATGGGAAGAGCTTGTGAAGAAAACTTCCAGTTTTTGCAACCAACCTCATACAATCAAGTACCAGCTCCCAAGCGAGGATCTTGATGCTCTTATATCCGTTTCTTCTGATGAAGATCTTCAAAATATGATAGAGGAGTACCATGGCCTCGAAAGGCATGAGGGTTCTCAAAGACCTCGAATCTTTTTGATTCCGTTAGGTGAATCTGAAAACACATCTTCCTTTGAGGCGGATAGCATACAGCAAAGTAACCCTGATTACCAATATGTTGCTGCTGTAAATGGCATGATAGATCCTAGTCCTAGGAAAAACATTGGAGGGAAGAATTTGACTACTGAAGCAAGTCAACAGGGAACCAACACTGTCCTATTCCCTATGGAAATTATGAGTGATTTTAAAGCTTTGCATCCTAATCAAATTTTAAGTGAACCCCAGGATATGACTCGGTCTGCCATTCAGTCTTCCTTTTCTCCCATTCTGCATCAGCGAGGAGATTCCAAGGGTGTTCATTTACAATCACATGGTCTAAACTCGTGCCAGGGCAGTAATGAGAGTAGCAGTTCTTTTATGAGTGCTCAACCACCACAGGATAACTCCAGCAATAGCACGGAAGGTTATAAAATTCATCCACAGGGGGTAGTAACTCTTATGGATTATCACCACCCTTGTAAGCAAGCTGATGATGGACAACTGGGCCTATACCATGGAGGACATAGTCTCAACCACAATCCCAGCAAAGATCCCATGTCTACTTTAGTTGTCGGTCAAAATGTTGGTGATTTTGATGGATTCTCCCATGAGATGCCAGTGCAGAAGGAAAGAATATTTCCTCCTGAGCCTGTCTCACACCAAGAAGATTCAAAGAATATGTTGTCCGGATCTAATGATTACGTTGATTGTCATCCTGGCATGCATCATGCATATTCTGATTCAAAACTGCAAGAGAATGGAGGGAGGTCTGTATATTGTTCACAAGAAGGAATGAGCCCACCGTCCCCCTTAATTTTTGCAAAGGCTCAATCATCATTGCTGCTGAACTCAGTTATTTCACAAGAAAAGCCGACTCTACTGCGTGACAATATTGAATCTTTGAATCCTAGGTTACACAATCAGTTACATGGTACGGAGTCGATAGGATTGCACAGCAGACTGGATTTGCCGAATTCATCTCCATGTTTAGAATCACTGGGCAGGAATGAAGATTCTCCCAAATGTAATGATTTTCATGAAAAATGCCGGACATTTAAACAGAAAGATTCTTTGACATTGGAGCAGACAAAGAAGGTCAATCAAAAGGATCCTTTCTTACATCAAGATGAAACCCTTTATGGGACAAGATCACCTCCCACTGAAGTTGATTATCAGAATGGATTTCCTAATATCATTCCCGACCCATCATCTACTTTTACCTCTGGGGTTGTTGTTCCTGCAACAATTAATTTGAAACCGTTGGTAAACAAGAAGGTGGAGGATTCACAAAGATTCCAGGGCGACAAAACCCCTGCCAATCTTCTTGTCACGAGTCAAAGAACTGCCAATGATCAGGACTGTGCTTTGGCAGGGATGCCTAGTGGTGAAAAAGGGCATGATGTTTCAGGGGCCAGGAATTCTGAAGTTGCAGGCATATTTCCAAGTACTGAACAGCATTCTCGTGATGAAAATTCTTTGGCTGATCTCATCTCAGGGTTGTCCAATGGCCAAGTTTCCCATGAGCCTGCACGACCAGAACTTGTTGCAAGTCAAAAGGATATGCGTTTCCAAGAACCCTTGCGTATGAACTCTGCACATGTGCACCCAGTGACAGTTCTTCATGATCCTGTACTGGAAAAGAGTGACCACATGGTGTTGCATAAGCCAGTTCAAGATGTTGCGTTTAAAAGACAGGTCTCTCTTCTTGATGATGACTTTGTAAATTACCCTGATAAGAATGCTGAGAAGTTGAGCTCAAATGTCGAAGATGTTAGTTTGGCACCAAAAAAACCCCCAACAATGAGGAATGATAAAAAACAGTTGGAGTCGGTGAAAATTGTGGAAGACATAACGAACGGTATTACTTCTGGCGTCCAGTCTTCATCACTAGTTCCCCCATATGCTGCGGATGAACCCGTTGGTGATCTCATATCTCCTACTACAACAGAGGTGGAGAGCGTCAATGAAGAGTCTGAGTATGAGGATGATAAGGCTGATGAAGAAGACAAAACTGAATCATTTAGTGATGCAATGATCGCTGAAATGGAAGCCAGCATCTATGGCTTGCAGATTATAAAAAATGCTGATCTTGAAGAATTACGGGAATTAGGATCTGGTACATATGGAACTGTTTATCATGGAAAATGGCGGGGAACAGATGTTGCTATAAAGCGAATAAAAAAGAGCTGTTTTGCAGGAAGATCATCAGAGCAAGAACGATTGACAAAAGACTTCTGGAGAGAGGCGCAAATCCTTTCAGCTCTCCATCATCCAAATGTGGTTGCATTCTATGGAGTAGTACCTGATGGAGCTGGAGGAACGTTGGCAACTGTGACTGAGTACATGGTGAATGGATCACTGAGGCATGCCCTACTAAAAAAGGATAGATCACTTGACCGTCGTAGAAAGCTTATAATTGCCATGGATGCAGCTTTTGGCATGGAATACTTGCATTCAAAGAATATTGTCCATTTTGATTTAAAGTGTGACAATCTGCTAGTCAACTTGAGGGATCCTCAACGACCTATATGCAAGGTTGGAGATTTTGGTTTATCGAGAATCAAACGCAATACTTTGGTATCTGGTGGTGTGCGAGGAACTCTTCCTTGGATGGCACCAGAACTGTTAAATGGCAGCAGCATCCGGGTTTCCGAGAAGGTTGATGTTTTCTCATTTGGAATTTCAATGTGGGAGATCTTGACCGGGGAGGAACCATATGCCAATATGCATTGCGGTGCCATCATTGGTGGAATTGTGAAGAACACTCTTCGACCTCCTATTCCAGAACGTTGTGATTCTGAGTGGAAGAACCTGATGGAACAGTGCTGGGCACCTGATCCTGACATCCGTCCATCATTCACCGAGATAACTAACAGGTTGCGAGCTATGTCCAACGCACTTCAGGCGAAGGTAGCTAGCAATCAGACAAGACAGATGAAACCCAATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1288

Amino Acids

142.36

Weight (kDa)

5.54

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 222 - 304 1.9e-21 PB1 domain
Pkinase PF00069 1002 - 1262 6.7e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1003 - 1264 4.1e-64 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 288, 1341, 2987, 3326
AccB1I GGYRCC 5 cut(s) 85, 2692, 3526, 3647, 3742
AccI GTMKAC 2 cut(s) 507, 1472
AccII CGCG 1 cut(s) 697
AccIII TCCGGA 1 cut(s) 1598
AciI CCGC 5 cut(s) 468, 953, 2819, 3061, 3645
AclI AACGTT 2 cut(s) 3233, 3700
AcoI YGGCCR 1 cut(s) 2419
AcsI RAATTY 9 cut(s) 188, 645, 1048, 1140, 1344, 1885, 2329, 2383, 3591
AcuI CTGAAG 6 cut(s) 191, 694, 1080, 2094, 2355, 3797
AcyI GRCGYC 1 cut(s) 2784
AdeI CACNNNGTG 2 cut(s) 2521, 2566
AfiI CCNNNNNNNGG 3 cut(s) 665, 799, 2052
AflIII ACRYGT 1 cut(s) 2509
AhlI ACTAGT 1 cut(s) 2800
AjnI CCWGG 7 cut(s) 88, 653, 1155, 1260, 1627, 2208, 2324
AjuI GAANNNNNNNTTGG 4 cut(s) 258, 290, 3502, 3534
Alw21I GWGCWC 4 cut(s) 60, 1300, 2517, 2669
Alw26I GTCTC 5 cut(s) 172, 686, 1445, 1570, 2614
Alw44I GTGCAC 1 cut(s) 2513
Aor13HI TCCGGA 1 cut(s) 1598
AoxI GGCC 5 cut(s) 656, 868, 1417, 2322, 2419
ApaLI GTGCAC 1 cut(s) 2513
ApeKI GCWGC 7 cut(s) 30, 995, 1753, 2816, 3341, 3546, 3549
ApoI RAATTY 9 cut(s) 188, 645, 1048, 1140, 1344, 1885, 2329, 2383, 3591
AseI ATTAAT 1 cut(s) 2160
Asp700I GAANNNNTTC 2 cut(s) 349, 840
AspA2I CCTAGG 2 cut(s) 1025, 1820
AspLEI GCGC 2 cut(s) 699, 3160
AspS9I GGNCC 5 cut(s) 51, 396, 524, 1417, 2322
AsuC2I CCSGG 2 cut(s) 3557, 3616
AsuNHI GCTAGC 1 cut(s) 3827
AvaII GGWCC 3 cut(s) 51, 396, 524
AvrII CCTAGG 2 cut(s) 1025, 1820
BaeGI GKGCMC 2 cut(s) 2517, 3745
BaeI ACNNNNGTAYC 2 cut(s) 3474, 3507
BalI TGGCCA 1 cut(s) 2421
BamHI GGATCC 3 cut(s) 108, 2020, 3424
BanI GGYRCC 5 cut(s) 85, 2692, 3526, 3647, 3742
BanII GRGCYC 2 cut(s) 1712, 2669
BauI CACGAG 3 cut(s) 1255, 2242, 2373
BbsI GAAGAC 4 cut(s) 1176, 2763, 2784, 2922
Bbv12I GWGCWC 4 cut(s) 60, 1300, 2517, 2669
BbvI GCAGC 7 cut(s) 17, 982, 1740, 2803, 3353, 3558, 3561
BcgI CGANNNNNNTGC 6 cut(s) 1774, 1808, 1843, 1877, 3423, 3457
BciT130I CCWGG 7 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326
BclI TGATCA 1 cut(s) 2263
BcnI CCSGG 2 cut(s) 3557, 3616
BcoDI GTCTC 5 cut(s) 172, 686, 1445, 1570, 2614
BcuI ACTAGT 1 cut(s) 2800
BfaI CTAG 8 cut(s) 660, 1020, 1026, 1821, 2292, 2801, 3410, 3828
BfmI CTRYAG 1 cut(s) 219
BglII AGATCT 2 cut(s) 838, 3606
BisI GCNGC 7 cut(s) 31, 996, 1754, 2817, 3342, 3547, 3550
BlnI CCTAGG 2 cut(s) 1025, 1820
BlsI GCNGC 7 cut(s) 32, 997, 1755, 2818, 3343, 3548, 3551
BmcAI AGTACT 1 cut(s) 2359
Bme1390I CCNGG 9 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326, 3557, 3616
Bme18I GGWCC 3 cut(s) 51, 396, 524
BmgT120I GGNCC 5 cut(s) 51, 396, 524, 1417, 2322
BmrFI CCNGG 9 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326, 3557, 3616
BmrI ACTGGG 3 cut(s) 1424, 1919, 2513
BmtI GCTAGC 1 cut(s) 3831
BmuI ACTGGG 3 cut(s) 1424, 1919, 2513
BoxI GACNNNNGTC 1 cut(s) 3759
BpiI GAAGAC 4 cut(s) 1176, 2763, 2784, 2922
BplI GAGNNNNNCTC 4 cut(s) 1550, 1582, 3412, 3444
BpmI CTGGAG 3 cut(s) 1303, 3169, 3246
Bpu10I CCTNAGC 1 cut(s) 1557
BpuEI CTTGAG 1 cut(s) 3439
BpuMI CCSGG 2 cut(s) 3557, 3616
BsaBI GATNNNNATC 2 cut(s) 837, 1623
BsaHI GRCGYC 1 cut(s) 2784
BsaI GGTCTC 1 cut(s) 2614
BsaWI WCCGGW 1 cut(s) 1598
Bsc4I CCNNNNNNNGG 3 cut(s) 665, 799, 2052
Bse1I ACTGG 9 cut(s) 753, 1419, 1529, 1878, 1914, 2519, 2550, 2579, 2788
Bse3DI GCAATG 3 cut(s) 1748, 2949, 3640
Bse8I GATNNNNATC 2 cut(s) 837, 1623
BseAI TCCGGA 1 cut(s) 1598
BseBI CCWGG 7 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326
BseJI GATNNNNATC 2 cut(s) 837, 1623
BseLI CCNNNNNNNGG 3 cut(s) 665, 799, 2052
BseMI GCAATG 3 cut(s) 1748, 2949, 3640
BseNI ACTGG 9 cut(s) 753, 1419, 1529, 1878, 1914, 2519, 2550, 2579, 2788
BseRI GAGGAG 4 cut(s) 355, 470, 872, 1227
BseSI GKGCMC 2 cut(s) 2517, 3745
BseXI GCAGC 7 cut(s) 17, 982, 1740, 2803, 3353, 3558, 3561
BseYI CCCAGC 6 cut(s) 12, 26, 304, 515, 1454, 3738
BsgI GTGCAG 3 cut(s) 1553, 2424, 2490
Bsh1236I CGCG 1 cut(s) 697
BshFI GGCC 5 cut(s) 658, 870, 1419, 2324, 2421
BshNI GGYRCC 5 cut(s) 85, 2692, 3526, 3647, 3742
BsiHKAI GWGCWC 4 cut(s) 60, 1300, 2517, 2669
BsiSI CCGG 4 cut(s) 1599, 1960, 3556, 3615
BslFI GGGAC 4 cut(s) 119, 541, 1702, 2068
BslI CCNNNNNNNGG 3 cut(s) 665, 799, 2052
BsmAI GTCTC 5 cut(s) 172, 686, 1445, 1570, 2614
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 4 cut(s) 119, 541, 1702, 2068
BsmI GAATGC 4 cut(s) 2368, 2656, 3197, 3364
BsnI GGCC 5 cut(s) 658, 870, 1419, 2324, 2421
Bso31I GGTCTC 1 cut(s) 2614
Bsp1286I GDGCHC 6 cut(s) 60, 1300, 1712, 2517, 2669, 3745
Bsp13I TCCGGA 1 cut(s) 1598
Bsp1407I TGTACA 2 cut(s) 154, 172
Bsp19I CCATGG 4 cut(s) 195, 865, 1426, 3333
BspACI CCGC 5 cut(s) 468, 953, 2819, 3061, 3645
BspANI GGCC 5 cut(s) 658, 870, 1419, 2324, 2421
BspEI TCCGGA 1 cut(s) 1598
BspFNI CGCG 1 cut(s) 697
BspHI TCATGA 2 cut(s) 1948, 2533
BspMAI CTGCAG 1 cut(s) 223
BspOI GCTAGC 1 cut(s) 3831
BspQI GCTCTTC 1 cut(s) 726
BspT107I GGYRCC 5 cut(s) 85, 2692, 3526, 3647, 3742
BspTNI GGTCTC 1 cut(s) 2614
BsrDI GCAATG 3 cut(s) 1748, 2949, 3640
BsrGI TGTACA 2 cut(s) 154, 172
BsrI ACTGG 9 cut(s) 753, 1419, 1529, 1878, 1914, 2519, 2550, 2579, 2788
BssNI GRCGYC 1 cut(s) 2784
BssSI CACGAG 3 cut(s) 1255, 2242, 2373
BssT1I CCWWGG 8 cut(s) 195, 865, 1025, 1221, 1426, 1820, 3333, 3518
Bst2BI CACGAG 3 cut(s) 1255, 2242, 2373
Bst2UI CCWGG 7 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326
Bst6I CTCTTC 6 cut(s) 223, 726, 2619, 2874, 3519, 3684
BstACI GRCGYC 1 cut(s) 2784
BstAUI TGTACA 2 cut(s) 154, 172
BstC8I GCNNGC 9 cut(s) 1398, 1634, 2344, 2439, 2965, 2978, 3279, 3795, 3829
BstDSI CCRYGG 4 cut(s) 195, 865, 1426, 3333
BstFNI CGCG 1 cut(s) 697
BstHHI GCGC 2 cut(s) 699, 3160
BstMAI GTCTC 5 cut(s) 172, 686, 1445, 1570, 2614
BstMWI GCNNNNNNNGC 5 cut(s) 2948, 2973, 3338, 3815, 3824
BstNI CCWGG 7 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326
BstNSI RCATGY 3 cut(s) 1636, 2513, 3281
BstPAI GACNNNNGTC 1 cut(s) 3759
BstSCI CCNGG 9 cut(s) 88, 653, 1155, 1260, 1627, 2208, 2324, 3555, 3614
BstSFI CTRYAG 1 cut(s) 219
BstSLI GKGCMC 2 cut(s) 2517, 3745
BstUI CGCG 1 cut(s) 697
BstV1I GCAGC 7 cut(s) 17, 982, 1740, 2803, 3353, 3558, 3561
BstV2I GAAGAC 4 cut(s) 1176, 2763, 2784, 2922
BsuRI GGCC 5 cut(s) 658, 870, 1419, 2324, 2421
BtgI CCRYGG 4 cut(s) 195, 865, 1426, 3333
BtgZI GCGATG 1 cut(s) 678
BtsIMutI CAGTG 8 cut(s) 570, 1083, 1536, 1907, 2070, 2526, 3269, 3740
Cac8I GCNNGC 9 cut(s) 1398, 1634, 2344, 2439, 2965, 2978, 3279, 3795, 3829
CciI TCATGA 2 cut(s) 1948, 2533
CfoI GCGC 2 cut(s) 699, 3160
Cfr13I GGNCC 5 cut(s) 51, 396, 524, 1417, 2322
CseI GACGC 3 cut(s) 703, 2773, 2860
CspCI CAANNNNNGTGG 4 cut(s) 2059, 2094, 2844, 2879
DraI TTTAAA 4 cut(s) 1120, 1969, 2599, 3393
DraIII CACNNNGTG 2 cut(s) 2521, 2566
EaeI YGGCCR 1 cut(s) 2419
Eam1104I CTCTTC 6 cut(s) 223, 726, 2619, 2874, 3519, 3684
EarI CTCTTC 6 cut(s) 223, 726, 2619, 2874, 3519, 3684
EciI GGCGGA 1 cut(s) 968
Ecl136II GAGCTC 1 cut(s) 2667
Eco130I CCWWGG 8 cut(s) 195, 865, 1025, 1221, 1426, 1820, 3333, 3518
Eco147I AGGCCT 1 cut(s) 658
Eco24I GRGCYC 2 cut(s) 1712, 2669
Eco31I GGTCTC 1 cut(s) 2614
Eco32I GATATC 1 cut(s) 311
Eco47I GGWCC 3 cut(s) 51, 396, 524
Eco53kI GAGCTC 1 cut(s) 2667
Eco57I CTGAAG 6 cut(s) 191, 694, 1080, 2094, 2355, 3797
EcoICRI GAGCTC 1 cut(s) 2667
EcoO109I RGGNCCY 1 cut(s) 396
EcoRI GAATTC 2 cut(s) 1885, 2329
EcoRII CCWGG 7 cut(s) 88, 653, 1155, 1260, 1627, 2208, 2324
EcoRV GATATC 1 cut(s) 311
EcoT14I CCWWGG 8 cut(s) 195, 865, 1025, 1221, 1426, 1820, 3333, 3518
EcoT22I ATGCAT 3 cut(s) 1638, 1645, 3642
EcoT38I GRGCYC 2 cut(s) 1712, 2669
ErhI CCWWGG 8 cut(s) 195, 865, 1025, 1221, 1426, 1820, 3333, 3518
Esp3I CGTCTC 1 cut(s) 686
FalI AAGNNNNNCTT 4 cut(s) 406, 438, 472, 504
FaqI GGGAC 4 cut(s) 119, 541, 1702, 2068
FauI CCCGC 1 cut(s) 3054
FauNDI CATATG 3 cut(s) 2812, 3034, 3628
FbaI TGATCA 1 cut(s) 2263
FblI GTMKAC 2 cut(s) 507, 1472
Fnu4HI GCNGC 7 cut(s) 31, 996, 1754, 2817, 3342, 3547, 3550
FriOI GRGCYC 2 cut(s) 1712, 2669
Fsp4HI GCNGC 7 cut(s) 31, 996, 1754, 2817, 3342, 3547, 3550
FspBI CTAG 8 cut(s) 660, 1020, 1026, 1821, 2292, 2801, 3410, 3828
GlaI GCGC 2 cut(s) 698, 3159
GluI GCNGC 7 cut(s) 31, 996, 1754, 2817, 3342, 3547, 3550
GsaI CCCAGC 6 cut(s) 16, 30, 308, 519, 1458, 3742
GsuI CTGGAG 3 cut(s) 1303, 3169, 3246
HaeIII GGCC 5 cut(s) 658, 870, 1419, 2324, 2421
HapII CCGG 4 cut(s) 1599, 1960, 3556, 3615
HgaI GACGC 3 cut(s) 703, 2773, 2860
HhaI GCGC 2 cut(s) 699, 3160
Hin1I GRCGYC 1 cut(s) 2784
Hin6I GCGC 2 cut(s) 697, 3158
HinP1I GCGC 2 cut(s) 697, 3158
HincII GTYRAC 3 cut(s) 508, 1070, 3415
HindII GTYRAC 3 cut(s) 508, 1070, 3415
HindIII AAGCTT 2 cut(s) 1122, 3320
HpaII CCGG 4 cut(s) 1599, 1960, 3556, 3615
Hpy166II GTNNAC 8 cut(s) 508, 1070, 1151, 1473, 1694, 2179, 2515, 3415
Hpy8I GTNNAC 8 cut(s) 508, 1070, 1151, 1473, 1694, 2179, 2515, 3415
Hpy99I CGWCG 2 cut(s) 127, 3315
HpyAV CCTTC 9 cut(s) 478, 718, 1328, 1531, 1694, 1999, 2179, 3562, 3814
HpyCH4IV ACGT 4 cut(s) 125, 1614, 3233, 3700
HpyF10VI GCNNNNNNNGC 5 cut(s) 2948, 2973, 3338, 3815, 3824
HpySE526I ACGT 4 cut(s) 125, 1614, 3233, 3700
Hsp92I GRCGYC 1 cut(s) 2784
HspAI GCGC 2 cut(s) 697, 3158
Kpn2I TCCGGA 1 cut(s) 1598
Ksp22I TGATCA 1 cut(s) 2263
LguI GCTCTTC 1 cut(s) 726
LmnI GCTCC 5 cut(s) 21, 587, 795, 1993, 3221
Lsp1109I GCAGC 7 cut(s) 17, 982, 1740, 2803, 3353, 3558, 3561
MaeI CTAG 8 cut(s) 660, 1020, 1026, 1821, 2292, 2801, 3410, 3828
MaeII ACGT 4 cut(s) 125, 1614, 3233, 3700
MhlI GDGCHC 6 cut(s) 60, 1300, 1712, 2517, 2669, 3745
MlsI TGGCCA 1 cut(s) 2421
MluNI TGGCCA 1 cut(s) 2421
MlyI GAGTC 7 cut(s) 563, 1159, 1780, 1859, 2254, 2747, 2891
MmeI TCCRAC 4 cut(s) 591, 2715, 3430, 3828
Mox20I TGGCCA 1 cut(s) 2421
Mph1103I ATGCAT 3 cut(s) 1638, 1645, 3642
MroI TCCGGA 1 cut(s) 1598
MroXI GAANNNNTTC 2 cut(s) 349, 840
MscI TGGCCA 1 cut(s) 2421
MseI TTAA 9 cut(s) 576, 1119, 1145, 1724, 1968, 2160, 2598, 3392, 3539
MslI CAYNNNNRTG 6 cut(s) 162, 1894, 2037, 2373, 3186, 3645
Msp20I TGGCCA 1 cut(s) 2421
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 4 cut(s) 1599, 1960, 3556, 3615
MspR9I CCNGG 9 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326, 3557, 3616
Mva1269I GAATGC 4 cut(s) 2368, 2656, 3197, 3364
MvaI CCWGG 7 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326
MvnI CGCG 1 cut(s) 697
MwoI GCNNNNNNNGC 5 cut(s) 2948, 2973, 3338, 3815, 3824
NciI CCSGG 2 cut(s) 3557, 3616
NcoI CCATGG 4 cut(s) 195, 865, 1426, 3333
NdeI CATATG 3 cut(s) 2812, 3034, 3628
NheI GCTAGC 1 cut(s) 3827
NmuCI GTSAC 7 cut(s) 241, 1796, 2239, 2521, 2555, 3244, 3398
NsiI ATGCAT 3 cut(s) 1638, 1645, 3642
NspI RCATGY 3 cut(s) 1636, 2513, 3281
PaeI GCATGC 2 cut(s) 1636, 3281
PagI TCATGA 2 cut(s) 1948, 2533
PceI AGGCCT 1 cut(s) 658
PciI ACATGT 1 cut(s) 2509
PciSI GCTCTTC 1 cut(s) 726
PctI GAATGC 4 cut(s) 2368, 2656, 3197, 3364
PdmI GAANNNNTTC 2 cut(s) 349, 840
PflFI GACNNNGTC 2 cut(s) 1168, 1437
PkrI GCNGC 7 cut(s) 32, 997, 1755, 2818, 3343, 3548, 3551
PleI GAGTC 7 cut(s) 562, 1159, 1780, 1858, 2253, 2746, 2890
PpsI GAGTC 7 cut(s) 562, 1159, 1780, 1858, 2253, 2746, 2890
PpuMI RGGWCCY 1 cut(s) 396
PscI ACATGT 1 cut(s) 2509
PshAI GACNNNNGTC 1 cut(s) 3759
PshBI ATTAAT 1 cut(s) 2160
PsiI TTATAA 4 cut(s) 288, 1341, 2987, 3326
Psp124BI GAGCTC 1 cut(s) 2669
Psp1406I AACGTT 2 cut(s) 3233, 3700
Psp5II RGGWCCY 1 cut(s) 396
Psp6I CCWGG 7 cut(s) 88, 653, 1155, 1260, 1627, 2208, 2324
PspFI CCCAGC 6 cut(s) 12, 26, 304, 515, 1454, 3738
PspGI CCWGG 7 cut(s) 88, 653, 1155, 1260, 1627, 2208, 2324
PspPI GGNCC 5 cut(s) 51, 396, 524, 1417, 2322
PspPPI RGGWCCY 1 cut(s) 396
PstI CTGCAG 1 cut(s) 223
PsyI GACNNNGTC 2 cut(s) 1168, 1437
PvuII CAGCTG 1 cut(s) 30
RseI CAYNNNNRTG 6 cut(s) 162, 1894, 2037, 2373, 3186, 3645
SacI GAGCTC 1 cut(s) 2669
SalI GTCGAC 1 cut(s) 506
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 9 cut(s) 576, 1119, 1145, 1724, 1968, 2160, 2598, 3392, 3539
SatI GCNGC 7 cut(s) 31, 996, 1754, 2817, 3342, 3547, 3550
Sau96I GGNCC 5 cut(s) 51, 396, 524, 1417, 2322
ScaI AGTACT 1 cut(s) 2359
SchI GAGTC 7 cut(s) 563, 1159, 1780, 1859, 2254, 2747, 2891
ScrFI CCNGG 9 cut(s) 90, 655, 1157, 1262, 1629, 2210, 2326, 3557, 3616
SduI GDGCHC 6 cut(s) 60, 1300, 1712, 2517, 2669, 3745
SfcI CTRYAG 1 cut(s) 219
SinI GGWCC 3 cut(s) 51, 396, 524
SmiMI CAYNNNNRTG 6 cut(s) 162, 1894, 2037, 2373, 3186, 3645
SmlI CTYRAG 1 cut(s) 3418
SmoI CTYRAG 1 cut(s) 3418
SpeI ACTAGT 1 cut(s) 2800
SphI GCATGC 2 cut(s) 1636, 3281
SseBI AGGCCT 1 cut(s) 658
SsiI CCGC 5 cut(s) 468, 953, 2819, 3061, 3645
SspI AATATT 3 cut(s) 1548, 1804, 3376
SspMI CTAG 8 cut(s) 660, 1020, 1026, 1821, 2292, 2801, 3410, 3828
SstI GAGCTC 1 cut(s) 2669
StuI AGGCCT 1 cut(s) 658
StyD4I CCNGG 9 cut(s) 88, 653, 1155, 1260, 1627, 2208, 2324, 3555, 3614
StyI CCWWGG 8 cut(s) 195, 865, 1025, 1221, 1426, 1820, 3333, 3518
TaiI ACGT 4 cut(s) 128, 1617, 3236, 3703
TaqI TCGA 7 cut(s) 507, 873, 901, 1853, 2676, 3467, 3682
TaqII GACCGA 2 cut(s) 1158, 1475
TatI WGTACW 5 cut(s) 154, 172, 2357, 2542, 3252
Tru1I TTAA 9 cut(s) 576, 1119, 1145, 1724, 1968, 2160, 2598, 3392, 3539
Tru9I TTAA 9 cut(s) 576, 1119, 1145, 1724, 1968, 2160, 2598, 3392, 3539
TscAI CASTG 8 cut(s) 577, 1090, 1536, 1914, 2077, 2526, 3276, 3740
TseFI GTSAC 7 cut(s) 241, 1796, 2239, 2521, 2555, 3244, 3398
TseI GCWGC 7 cut(s) 30, 995, 1753, 2816, 3341, 3546, 3549
Tsp45I GTSAC 7 cut(s) 241, 1796, 2239, 2521, 2555, 3244, 3398
TspGWI ACGGA 5 cut(s) 811, 906, 1346, 1862, 3749
TspRI CASTG 8 cut(s) 577, 1090, 1536, 1914, 2077, 2526, 3276, 3740
Tth111I GACNNNGTC 2 cut(s) 1168, 1437
VneI GTGCAC 1 cut(s) 2513
VpaK11BI GGWCC 3 cut(s) 51, 396, 524
VspI ATTAAT 1 cut(s) 2160
XapI RAATTY 9 cut(s) 188, 645, 1048, 1140, 1344, 1885, 2329, 2383, 3591
XceI RCATGY 3 cut(s) 1636, 2513, 3281
XmaJI CCTAGG 2 cut(s) 1025, 1820
XmiI GTMKAC 2 cut(s) 507, 1472
XmnI GAANNNNTTC 2 cut(s) 349, 840
XspI CTAG 8 cut(s) 660, 1020, 1026, 1821, 2292, 2801, 3410, 3828
ZrmI AGTACT 1 cut(s) 2359
Zsp2I ATGCAT 3 cut(s) 1638, 1645, 3642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.