MD10G1168800.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009051
Physical Location & Seq
Reverse (-)
26076199 .. 26083273
7075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1168800.v1.1.491

Sequence Viewer

Length: 3831 bp
ATGCAGCTAAGTAGGTCCGAGCACTTGTTTGATACCATGGCCAATGAGGTGCCTGGCCCATCAGGTCAATGGATCCAGAGAGAAGCGAAGTTTGTTGTCCCAAAAACAGTAAAGAATGTGCATAAGAATGTCTCTGTGCAGACAGGTGAGGAATTTTCCATGGAGTTTCTTCAGGATCGATCTGCTGCAGGAAGAGTTCCTGCTGTGTCAGATATGGTTGAAAATTGTGAAAATGGGGTTTTGTCGAATTATAATAAGAATAAGCAGCTGGGATATCAGGATTGTACTGGTCTTCTGGGGTTGAGAAGACTGGATTATGAGTGTGCATCTGATATGTCTGAGTTTGTCTCGGCAAAAGGGTTGTGTAAAGATATTGAAAGCGAGGCTTGTGTTGACAAGTTAGGCAGATGCAACAGGGAGGAGGTTGATAGCGGACAAGGATCAAGGAAGGCTATTGGAGAACTGAATGTTGACAGAGTTGGTTTTGGACCAACTGCCTTACCTATATTTATGTCTGAGTCCCCTCATTCCAACACTGTCAATGGGTCAGGAGCGGTGGATAGTTCTCAATCTGGGAAGCTGAAGTTTCTTTGCAGTTTTGGTGGAAAAATCTTGCCCAGGCCTAGCGATGGAAAACTCAGATATGTTGGGGGAGAGACGCGCATTATTTCCTTCAGGAAAAGTATTTCATGGGAAGAGCTTGTGAAGAAAACTACCAGTTTTTGCAACCAGCCTCATACAATCAAGTACCAGCTCCCAAGTGAGGATCTTGATTCTCTTATATCCATGTCTTCTGATGAAGATCTTCAAAATATGATGGAGGAGTACCATGGGCTCGAAAGGCATGAGGGTTCTCAGAGACCTCGAATCTTTTTGATTCCTTTAGGTGAATCTGAAAACACATCTTCCTTTGAGGCGGATAGCATACAGCAATGTAACCCTGATTACCAGTATGTTGCTGCTGTAAATGGCATGATAGGTCCTAGTCCTAGGAAAAACAGTGGAGGGCAGAATTTGACTACTGAAGCAAGTCAACAAGGAACCAAGACTGTCCTATTTTCTATGGAAATTAAGAGTGACCTTAAAGCTCTGCATCCCAATCAAATTTTAAGTGAACCCCAGAATATTAATCGGTCTGCCATTCAATCTCCCTTTTCTCTAATTCTGCATCAGCGAGGAGGTTCCAAGGGTGTTCATTTACAATCATATGGTCTAAACTCATGCCAGGGTAGTAATGAGAGTAGCAGTTCTTTTATAAGTGCTCAACCACCACAAGAGAATTCCAGCAATAGCACGGAAGGTTATAAAATTCATCCACAGGGGGCAGTAACTGTTATGGACTATCACCACCCTCGTAAGCAAGCTGATGATGCTCAACCGGGCCAATACCATGGAGGACATAGTCTCAACCACAATCCCAGCAAAGATCCCATGTCTACTTTAGCTGTCGGTCAAAAGGTTGGTGATTTTGATGGATTCTCCCATGAGAGGCCCATGCATGAGGAAAGAATATGTCCTCCTGAGCCTATCTCACACCAAGAAGATTCAAAGATTATGTTGTCAGAATCTAATGATTATGTTGATTGTCATTGTGGCATGCATCATGCATATTCCGATTCAAAACTGCATGAGAATGGAGGGAGGTCTGTATACTGTTCACAAGAAGGAATAAGCTCATCGTCCCCCTTAAGTTTTGCAAAGGCTCAATCGTCATCGCTGCTGAACTCAGGTATTTCACAAGAAAAGCCGACCCTACTGTGTGACAATATTGAATCTTTGAATCCTCGGTTACACAACCAGTTACATGGTATGGAGTCAATAGGATTGCAGAGCAGACTGGATTTGCCGAACTCATCTCCATGTTTAGAATCACTGGGCAAGAATGAACATACTCCCAAATGTAATGGTGATTTCCATGAAAAATGCCGGACAGCTAAAAAGAAAGATTCTCTGACTTTGGATCTGACGAAGAAGGTCAATCAAAAGGATCCTTTCTTACATCAAGATGAAACCCTTTATGGGACAAGATCACCTGCCACTGGAGTTGATTATCGGAATGGATTTCCTAATATTATTCCCAACCCATCATCTACTTTTGCCTCTGGGGTTGTTGTTCCTGCAGCAATTAATTTGAAACCGTTGGTAAACAACAAGGTGGAGGATTCACAAAGCTTCCAGTGCGACAAAACCCCTGCCGATCTTCTTGTTACGAGTCAAAGAACTGCCAGTGATCAGGACTGTGCTTTGGCAGGGATGCCTAGTTGTGAACAAGGACATGATGTTTCAGGGGCCAGGAATTCTGAAGTTGCAGGCATATTTCCAAGCACTAAACAGCATTCTCGTGGTGAAAATTCTTTGGCTGATCTCATCTCAGGGTTGTCCAATGGCCCAGTTTCCCATGAGCCTGCACGACCACAACTTGTTGCAAGTCAAAAGGATATGGGTTTCCAAGAACCCCTGCCTATAAACTCTGCAAATATGCATCCAATGACAGTTCTTCGTGATCCTGTGCTGGAAAAGAGTGACCACATGGTGTTGCCTAATCCACCCCAAGATGTTGTGTTTAAAAGACAGGTCTCTCTTCTCGATGATGACTTTGTAAATTACCCTGATAAGAATGTTGAGAAGTTGAGCTCACATGTCGAAGATGTTTGTTTGGCACAATCAAAACCCCCAACAATGAGGAATGATAAAAAGCAGTTGGAGTCGGTGATAAATGTGGAAGACATAACTAATGGTGTTACTTCTGGCATCCAGTCTTCATCACCAGTTTCCCCATATGCTTTCGACGAACCCATTGGTGATCTCATATCTCCTACTGCAACAGAGGTGGAGAGCATCAATCCAGATTCTGAGTATGAGGATGATAAGGCTGATGAAGACAACAAAAATGAAACATTTAGTGATGCAATGATCGCTGAAATGGAAGCCAGCATCTATGGCTTGCAGATTATAAAAAATGCTGATCTTGAAGAATTACGGGAATTAGGTTCTGGTACATATGGAACTGTTTATCATGGAAAATGGCGGGGAACAGATGTTGCTATAAAGCGAATAAAAAAGAGCTGTTTTGCAGGAAGATCATCAGAGCAAGACCGTCTGACAAAAGACTTCTGGAGAGAGGCGCAAATCCTTTCAGCTCTCCATCATCCAAATGTGGTTGCATTATATGGGGTAGTACCTGATGGAGCTGGAGGAACGTTGGCAACTGTAACTGAGTACATGGTGAACGGATCACTTAGGCATGCCCTACTTAAAAAGAATAGATCACTTGACCGTCGTAGAAAGCTTATAATTGCCATGGATGCTGCTTTTGGCATGGAATACTTGCATTCAAAGAATATTGTTCATTTTGATTTGAAGTGTGACAATCTGCTAGTCAACTTGAGGGATCCTCAACGACCTATATGCAAGGTTGGAGATTTTGGTTTATCGAGAATCAAACGCAATACTTTGGTATCTGGTGGTGTGCGAGGAACTCTTCCTTGGATGGCACCAGAACTGTTAAATGGCAGCAGCAGCCGGGTTTCTGAGAAGGTTGATGTTTTCTCATTTGGAATTTCACTATGGGAGATCTTGACCGGGGAGGAACCATATGCCAATATGCATTGTGGTGCCATCATTGGTGGGATTGTGAAGAACATTCTTCGACCTCCTATTCCTGAACGGTGTGATTCCGGGTGGAAGAACCTGATGGAGCAGTGCTGGTCACCTGATCCTGGCATCCGTCCATCATTCACCGAGATAACTAAGAGGTTGCGAGCTATGTCCAATGCACTTCAGATGAAGGTACCTAGCAATCAGACGAGACAGAGGAAGCCCAACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1277

Amino Acids

140.43

Weight (kDa)

5.83

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 210 - 292 2.1e-20 PB1 domain
Pkinase PF00069 991 - 1251 2e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 992 - 1253 3e-62 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 252, 1256, 1305, 2954, 3293
AarI CACCTGC 1 cut(s) 2041
Acc36I ACCTGC 1 cut(s) 2041
Acc65I GGTACC 1 cut(s) 3790
AccB1I GGYRCC 4 cut(s) 49, 3493, 3614, 3790
AccBSI CCGCTC 1 cut(s) 554
AccI GTMKAC 2 cut(s) 1436, 1650
AccII CGCG 1 cut(s) 661
AciI CCGC 4 cut(s) 432, 554, 917, 3028
AclI AACGTT 2 cut(s) 3200, 3825
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 8 cut(s) 152, 1012, 1104, 1279, 1308, 2296, 2350, 3558
AcuI CTGAAG 6 cut(s) 155, 602, 658, 1044, 2322, 3764
AdeI CACNNNGTG 1 cut(s) 2533
AfaI GTAC 7 cut(s) 286, 749, 827, 2998, 3180, 3221, 3792
AfiI CCNNNNNNNGG 6 cut(s) 629, 763, 1488, 2019, 2039, 3719
AflII CTTAAG 1 cut(s) 1687
AflIII ACRYGT 1 cut(s) 2638
AjnI CCWGG 5 cut(s) 52, 617, 1224, 2291, 3718
AjuI GAANNNNNNNTTGG 2 cut(s) 3469, 3501
Alw21I GWGCWC 3 cut(s) 24, 1264, 2636
Alw26I GTCTC 7 cut(s) 136, 352, 650, 853, 1409, 2581, 3802
AlwNI CAGNNNCTG 2 cut(s) 1331, 2852
AoxI GGCC 7 cut(s) 39, 55, 620, 1381, 1490, 2289, 2386
ApoI RAATTY 8 cut(s) 152, 1012, 1104, 1279, 1308, 2296, 2350, 3558
AseI ATTAAT 2 cut(s) 1128, 2127
Asp700I GAANNNNTTC 1 cut(s) 804
Asp718I GGTACC 1 cut(s) 3790
AspA2I CCTAGG 1 cut(s) 989
AspLEI GCGC 2 cut(s) 663, 3127
AspS9I GGNCC 8 cut(s) 15, 56, 488, 980, 1381, 1491, 2289, 2387
AsuC2I CCSGG 4 cut(s) 1380, 3524, 3583, 3679
AvaII GGWCC 3 cut(s) 15, 488, 980
AvrII CCTAGG 1 cut(s) 989
BaeI ACNNNNGTAYC 2 cut(s) 3441, 3474
BalI TGGCCA 1 cut(s) 41
BamHI GGATCC 3 cut(s) 72, 1987, 3391
BanI GGYRCC 4 cut(s) 49, 3493, 3614, 3790
BanII GRGCYC 2 cut(s) 837, 2636
BauI CACGAG 1 cut(s) 2340
BbsI GAAGAC 6 cut(s) 284, 313, 783, 2730, 2751, 2886
Bbv12I GWGCWC 3 cut(s) 24, 1264, 2636
BcgI CGANNNNNNTGC 2 cut(s) 3390, 3424
BciT130I CCWGG 5 cut(s) 54, 619, 1226, 2293, 3720
BclI TGATCA 1 cut(s) 2230
BcnI CCSGG 4 cut(s) 1380, 3524, 3583, 3679
BcoDI GTCTC 7 cut(s) 136, 352, 650, 853, 1409, 2581, 3802
BfaI CTAG 6 cut(s) 624, 984, 990, 2259, 3377, 3795
BfmI CTRYAG 2 cut(s) 186, 2118
BfrI CTTAAG 1 cut(s) 1687
BfuAI ACCTGC 1 cut(s) 2041
BglII AGATCT 2 cut(s) 802, 3573
BlnI CCTAGG 1 cut(s) 989
Bme1390I CCNGG 9 cut(s) 54, 619, 1226, 1380, 2293, 3524, 3583, 3679, 3720
Bme18I GGWCC 3 cut(s) 15, 488, 980
BmgT120I GGNCC 8 cut(s) 15, 56, 488, 980, 1381, 1491, 2289, 2387
BmrFI CCNGG 9 cut(s) 54, 619, 1226, 1380, 2293, 3524, 3583, 3679, 3720
BmrI ACTGGG 2 cut(s) 1883, 2384
BmuI ACTGGG 2 cut(s) 1883, 2384
BpiI GAAGAC 6 cut(s) 284, 313, 783, 2730, 2751, 2886
BplI GAGNNNNNCTC 6 cut(s) 332, 364, 1514, 1546, 3379, 3411
BpmI CTGGAG 3 cut(s) 2061, 3136, 3213
Bpu10I CCTNAGC 1 cut(s) 1521
BpuEI CTTGAG 1 cut(s) 3406
BpuMI CCSGG 4 cut(s) 1380, 3524, 3583, 3679
Bsa29I ATCGAT 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 801
BsaI GGTCTC 2 cut(s) 853, 2581
Bsc4I CCNNNNNNNGG 6 cut(s) 629, 763, 1488, 2019, 2039, 3719
Bse3DI GCAATG 2 cut(s) 938, 2916
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 5 cut(s) 54, 619, 1226, 2293, 3720
BseCI ATCGAT 1 cut(s) 178
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 6 cut(s) 629, 763, 1488, 2019, 2039, 3719
BseMI GCAATG 2 cut(s) 938, 2916
BseRI GAGGAG 3 cut(s) 434, 836, 1191
BseYI CCCAGC 2 cut(s) 268, 1418
BsgI GTGCAG 2 cut(s) 158, 2391
Bsh1236I CGCG 1 cut(s) 661
BshFI GGCC 7 cut(s) 41, 57, 622, 1383, 1492, 2291, 2388
BshNI GGYRCC 4 cut(s) 49, 3493, 3614, 3790
BshVI ATCGAT 1 cut(s) 178
BsiHKAI GWGCWC 3 cut(s) 24, 1264, 2636
BsiSI CCGG 5 cut(s) 1379, 1927, 3523, 3582, 3678
BslFI GGGAC 4 cut(s) 83, 505, 1666, 2035
BslI CCNNNNNNNGG 6 cut(s) 629, 763, 1488, 2019, 2039, 3719
BsmAI GTCTC 7 cut(s) 136, 352, 650, 853, 1409, 2581, 3802
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 4 cut(s) 83, 505, 1666, 2035
BsmI GAATGC 2 cut(s) 2335, 3331
BsnI GGCC 7 cut(s) 41, 57, 622, 1383, 1492, 2291, 2388
Bso31I GGTCTC 2 cut(s) 853, 2581
Bsp1286I GDGCHC 4 cut(s) 24, 837, 1264, 2636
Bsp19I CCATGG 5 cut(s) 36, 159, 829, 1390, 3300
BspACI CCGC 4 cut(s) 432, 554, 917, 3028
BspANI GGCC 7 cut(s) 41, 57, 622, 1383, 1492, 2291, 2388
BspDI ATCGAT 1 cut(s) 178
BspFNI CGCG 1 cut(s) 661
BspMAI CTGCAG 2 cut(s) 190, 2122
BspMI ACCTGC 1 cut(s) 2041
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 4 cut(s) 49, 3493, 3614, 3790
BspTI CTTAAG 1 cut(s) 1687
BspTNI GGTCTC 2 cut(s) 853, 2581
BsrBI CCGCTC 1 cut(s) 554
BsrDI GCAATG 2 cut(s) 938, 2916
BssNAI GTATAC 1 cut(s) 1651
BssSI CACGAG 1 cut(s) 2340
BssT1I CCWWGG 8 cut(s) 36, 159, 829, 989, 1185, 1390, 3300, 3485
Bst1107I GTATAC 1 cut(s) 1651
Bst2BI CACGAG 1 cut(s) 2340
Bst2UI CCWGG 5 cut(s) 54, 619, 1226, 2293, 3720
Bst6I CTCTTC 4 cut(s) 187, 690, 2586, 3486
BstAFI CTTAAG 1 cut(s) 1687
BstC8I GCNNGC 8 cut(s) 1362, 1598, 2311, 2406, 2932, 2945, 3246, 3762
BstDSI CCRYGG 5 cut(s) 36, 159, 829, 1390, 3300
BstEII GGTNACC 1 cut(s) 3708
BstFNI CGCG 1 cut(s) 661
BstHHI GCGC 2 cut(s) 663, 3127
BstMAI GTCTC 7 cut(s) 136, 352, 650, 853, 1409, 2581, 3802
BstMWI GCNNNNNNNGC 7 cut(s) 841, 1370, 2178, 2915, 2940, 3305, 3519
BstNI CCWGG 5 cut(s) 54, 619, 1226, 2293, 3720
BstNSI RCATGY 3 cut(s) 1600, 2642, 3248
BstPI GGTNACC 1 cut(s) 3708
BstSCI CCNGG 9 cut(s) 52, 617, 1224, 1378, 2291, 3522, 3581, 3677, 3718
BstSFI CTRYAG 2 cut(s) 186, 2118
BstUI CGCG 1 cut(s) 661
BstV2I GAAGAC 6 cut(s) 284, 313, 783, 2730, 2751, 2886
BstX2I RGATCY 8 cut(s) 72, 766, 802, 1426, 1960, 1987, 3391, 3573
BstXI CCANNNNNNTGG 2 cut(s) 1391, 1805
BstYI RGATCY 8 cut(s) 72, 766, 802, 1426, 1960, 1987, 3391, 3573
BstZ17I GTATAC 1 cut(s) 1651
Bsu15I ATCGAT 1 cut(s) 178
BsuRI GGCC 7 cut(s) 41, 57, 622, 1383, 1492, 2291, 2388
BsuTUI ATCGAT 1 cut(s) 178
BtgI CCRYGG 5 cut(s) 36, 159, 829, 1390, 3300
BtgZI GCGATG 2 cut(s) 642, 1698
BtsI GCAGTG 1 cut(s) 3707
BtsIMutI CAGTG 7 cut(s) 534, 1006, 1871, 2037, 2183, 2233, 3707
BveI ACCTGC 1 cut(s) 2041
Cac8I GCNNGC 8 cut(s) 1362, 1598, 2311, 2406, 2932, 2945, 3246, 3762
CaiI CAGNNNCTG 2 cut(s) 1331, 2852
CfoI GCGC 2 cut(s) 663, 3127
Cfr13I GGNCC 8 cut(s) 15, 56, 488, 980, 1381, 1491, 2289, 2387
ClaI ATCGAT 1 cut(s) 178
CseI GACGC 1 cut(s) 667
Csp6I GTAC 7 cut(s) 285, 748, 826, 2997, 3179, 3220, 3791
CspCI CAANNNNNGTGG 4 cut(s) 2026, 2061, 2811, 2846
CviQI GTAC 7 cut(s) 285, 748, 826, 2997, 3179, 3220, 3791
DraI TTTAAA 1 cut(s) 2566
DraIII CACNNNGTG 1 cut(s) 2533
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 4 cut(s) 187, 690, 2586, 3486
EarI CTCTTC 4 cut(s) 187, 690, 2586, 3486
EciI GGCGGA 1 cut(s) 932
Ecl136II GAGCTC 1 cut(s) 2634
Eco130I CCWWGG 8 cut(s) 36, 159, 829, 989, 1185, 1390, 3300, 3485
Eco147I AGGCCT 1 cut(s) 622
Eco24I GRGCYC 2 cut(s) 837, 2636
Eco31I GGTCTC 2 cut(s) 853, 2581
Eco32I GATATC 1 cut(s) 275
Eco47I GGWCC 3 cut(s) 15, 488, 980
Eco53kI GAGCTC 1 cut(s) 2634
Eco57I CTGAAG 6 cut(s) 155, 602, 658, 1044, 2322, 3764
Eco91I GGTNACC 1 cut(s) 3708
EcoICRI GAGCTC 1 cut(s) 2634
EcoO109I RGGNCCY 1 cut(s) 980
EcoO65I GGTNACC 1 cut(s) 3708
EcoRI GAATTC 2 cut(s) 1279, 2296
EcoRII CCWGG 5 cut(s) 52, 617, 1224, 2291, 3718
EcoRV GATATC 1 cut(s) 275
EcoT14I CCWWGG 8 cut(s) 36, 159, 829, 989, 1185, 1390, 3300, 3485
EcoT22I ATGCAT 5 cut(s) 1500, 1602, 1609, 2484, 3609
EcoT38I GRGCYC 2 cut(s) 837, 2636
ErhI CCWWGG 8 cut(s) 36, 159, 829, 989, 1185, 1390, 3300, 3485
Esp3I CGTCTC 1 cut(s) 650
FalI AAGNNNNNCTT 2 cut(s) 370, 402
FaqI GGGAC 4 cut(s) 83, 505, 1666, 2035
FauI CCCGC 1 cut(s) 3021
FauNDI CATATG 4 cut(s) 1207, 2779, 3001, 3595
FbaI TGATCA 1 cut(s) 2230
FblI GTMKAC 2 cut(s) 1436, 1650
FriOI GRGCYC 2 cut(s) 837, 2636
FspBI CTAG 6 cut(s) 624, 984, 990, 2259, 3377, 3795
GlaI GCGC 2 cut(s) 662, 3126
GsaI CCCAGC 2 cut(s) 272, 1422
GsuI CTGGAG 3 cut(s) 2061, 3136, 3213
HaeIII GGCC 7 cut(s) 41, 57, 622, 1383, 1492, 2291, 2388
HapII CCGG 5 cut(s) 1379, 1927, 3523, 3582, 3678
HgaI GACGC 1 cut(s) 667
HhaI GCGC 2 cut(s) 663, 3127
Hin6I GCGC 2 cut(s) 661, 3125
HinP1I GCGC 2 cut(s) 661, 3125
HincII GTYRAC 4 cut(s) 394, 472, 1034, 3382
HindII GTYRAC 4 cut(s) 394, 472, 1034, 3382
HindIII AAGCTT 2 cut(s) 2170, 3287
HpaII CCGG 5 cut(s) 1379, 1927, 3523, 3582, 3678
Hpy99I CGWCG 2 cut(s) 2792, 3282
HpyAV CCTTC 7 cut(s) 442, 682, 1292, 1658, 1966, 3529, 3781
HpyCH4IV ACGT 2 cut(s) 3200, 3825
HpyF10VI GCNNNNNNNGC 7 cut(s) 841, 1370, 2178, 2915, 2940, 3305, 3519
HpySE526I ACGT 2 cut(s) 3200, 3825
HspAI GCGC 2 cut(s) 661, 3125
KpnI GGTACC 1 cut(s) 3794
Ksp22I TGATCA 1 cut(s) 2230
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 4 cut(s) 551, 759, 3188, 3697
MaeI CTAG 6 cut(s) 624, 984, 990, 2259, 3377, 3795
MaeII ACGT 2 cut(s) 3200, 3825
MbiI CCGCTC 1 cut(s) 554
MflI RGATCY 8 cut(s) 72, 766, 802, 1426, 1960, 1987, 3391, 3573
MhlI GDGCHC 4 cut(s) 24, 837, 1264, 2636
MlsI TGGCCA 1 cut(s) 41
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 4 cut(s) 527, 1823, 2221, 2714
MmeI TCCRAC 3 cut(s) 555, 2682, 3397
Mox20I TGGCCA 1 cut(s) 41
Mph1103I ATGCAT 5 cut(s) 1500, 1602, 1609, 2484, 3609
MroXI GAANNNNTTC 1 cut(s) 804
MscI TGGCCA 1 cut(s) 41
MseI TTAA 9 cut(s) 1071, 1083, 1109, 1128, 1688, 2127, 2565, 3255, 3506
MslI CAYNNNNRTG 7 cut(s) 126, 1632, 1858, 2004, 2340, 3153, 3612
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 268
MspCI CTTAAG 1 cut(s) 1687
MspI CCGG 5 cut(s) 1379, 1927, 3523, 3582, 3678
MspR9I CCNGG 9 cut(s) 54, 619, 1226, 1380, 2293, 3524, 3583, 3679, 3720
Mva1269I GAATGC 2 cut(s) 2335, 3331
MvaI CCWGG 5 cut(s) 54, 619, 1226, 2293, 3720
MvnI CGCG 1 cut(s) 661
MwoI GCNNNNNNNGC 7 cut(s) 841, 1370, 2178, 2915, 2940, 3305, 3519
NciI CCSGG 4 cut(s) 1380, 3524, 3583, 3679
NcoI CCATGG 5 cut(s) 36, 159, 829, 1390, 3300
NdeI CATATG 4 cut(s) 1207, 2779, 3001, 3595
NmeAIII GCCGAG 1 cut(s) 329
NmuCI GTSAC 5 cut(s) 1076, 1760, 2522, 3365, 3708
NsiI ATGCAT 5 cut(s) 1500, 1602, 1609, 2484, 3609
NspI RCATGY 3 cut(s) 1600, 2642, 3248
PaeI GCATGC 2 cut(s) 1600, 3248
PaqCI CACCTGC 1 cut(s) 2041
PceI AGGCCT 1 cut(s) 622
PciI ACATGT 1 cut(s) 2638
PciSI GCTCTTC 1 cut(s) 690
PctI GAATGC 2 cut(s) 2335, 3331
PdmI GAANNNNTTC 1 cut(s) 804
PflFI GACNNNGTC 1 cut(s) 1401
PleI GAGTC 4 cut(s) 526, 1822, 2220, 2713
PpsI GAGTC 4 cut(s) 526, 1822, 2220, 2713
PpuMI RGGWCCY 1 cut(s) 980
PscI ACATGT 1 cut(s) 2638
PshBI ATTAAT 2 cut(s) 1128, 2127
PsiI TTATAA 5 cut(s) 252, 1256, 1305, 2954, 3293
Psp124BI GAGCTC 1 cut(s) 2636
Psp1406I AACGTT 2 cut(s) 3200, 3825
Psp5II RGGWCCY 1 cut(s) 980
Psp6I CCWGG 5 cut(s) 52, 617, 1224, 2291, 3718
PspEI GGTNACC 1 cut(s) 3708
PspFI CCCAGC 2 cut(s) 268, 1418
PspGI CCWGG 5 cut(s) 52, 617, 1224, 2291, 3718
PspPI GGNCC 8 cut(s) 15, 56, 488, 980, 1381, 1491, 2289, 2387
PspPPI RGGWCCY 1 cut(s) 980
PstI CTGCAG 2 cut(s) 190, 2122
PstNI CAGNNNCTG 2 cut(s) 1331, 2852
PsuI RGATCY 8 cut(s) 72, 766, 802, 1426, 1960, 1987, 3391, 3573
PsyI GACNNNGTC 1 cut(s) 1401
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 7 cut(s) 286, 749, 827, 2998, 3180, 3221, 3792
RsaNI GTAC 7 cut(s) 285, 748, 826, 2997, 3179, 3220, 3791
RseI CAYNNNNRTG 7 cut(s) 126, 1632, 1858, 2004, 2340, 3153, 3612
SacI GAGCTC 1 cut(s) 2636
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 9 cut(s) 1071, 1083, 1109, 1128, 1688, 2127, 2565, 3255, 3506
Sau96I GGNCC 8 cut(s) 15, 56, 488, 980, 1381, 1491, 2289, 2387
SchI GAGTC 4 cut(s) 527, 1823, 2221, 2714
ScrFI CCNGG 9 cut(s) 54, 619, 1226, 1380, 2293, 3524, 3583, 3679, 3720
SduI GDGCHC 4 cut(s) 24, 837, 1264, 2636
SfcI CTRYAG 2 cut(s) 186, 2118
SinI GGWCC 3 cut(s) 15, 488, 980
SmiMI CAYNNNNRTG 7 cut(s) 126, 1632, 1858, 2004, 2340, 3153, 3612
SmlI CTYRAG 2 cut(s) 1687, 3385
SmoI CTYRAG 2 cut(s) 1687, 3385
SphI GCATGC 2 cut(s) 1600, 3248
SseBI AGGCCT 1 cut(s) 622
SsiI CCGC 4 cut(s) 432, 554, 917, 3028
SspI AATATT 4 cut(s) 1126, 1768, 2071, 3343
SspMI CTAG 6 cut(s) 624, 984, 990, 2259, 3377, 3795
SstI GAGCTC 1 cut(s) 2636
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 9 cut(s) 52, 617, 1224, 1378, 2291, 3522, 3581, 3677, 3718
StyI CCWWGG 8 cut(s) 36, 159, 829, 989, 1185, 1390, 3300, 3485
TaiI ACGT 2 cut(s) 3203, 3828
TaqI TCGA 9 cut(s) 178, 245, 837, 865, 2586, 2643, 2787, 3434, 3649
TaqII GACCGA 2 cut(s) 1122, 1439
TatI WGTACW 2 cut(s) 284, 3219
Tru1I TTAA 9 cut(s) 1071, 1083, 1109, 1128, 1688, 2127, 2565, 3255, 3506
Tru9I TTAA 9 cut(s) 1071, 1083, 1109, 1128, 1688, 2127, 2565, 3255, 3506
TscAI CASTG 7 cut(s) 541, 1006, 1878, 2044, 2183, 2233, 3707
TseFI GTSAC 5 cut(s) 1076, 1760, 2522, 3365, 3708
Tsp45I GTSAC 5 cut(s) 1076, 1760, 2522, 3365, 3708
TspGWI ACGGA 3 cut(s) 1310, 3246, 3716
TspRI CASTG 7 cut(s) 541, 1006, 1878, 2044, 2183, 2233, 3707
Tth111I GACNNNGTC 1 cut(s) 1401
Vha464I CTTAAG 1 cut(s) 1687
VpaK11BI GGWCC 3 cut(s) 15, 488, 980
VspI ATTAAT 2 cut(s) 1128, 2127
XapI RAATTY 8 cut(s) 152, 1012, 1104, 1279, 1308, 2296, 2350, 3558
XceI RCATGY 3 cut(s) 1600, 2642, 3248
XcmI CCANNNNNNNNNTGG 1 cut(s) 66
XmaJI CCTAGG 1 cut(s) 989
XmiI GTMKAC 2 cut(s) 1436, 1650
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 6 cut(s) 624, 984, 990, 2259, 3377, 3795
Zsp2I ATGCAT 5 cut(s) 1500, 1602, 1609, 2484, 3609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.