Rh6BG284500

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009051
Physical Location & Seq
Reverse (-)
50823045 .. 50830324
7280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG284500.1

Sequence Viewer

Length: 3258 bp
ATGCAGCTAAGTAGGTCTGAGAACTTGCTTGGTACCATGACCGGTGAGGTGTCATGCTCATCAGGTCAATGGATCCAGCAAGAGTCGACCCCAGTTGTCCAGAATATGGGAAAGAATGTGCGCAGAAATGTCTCTGTGCAGACAGGGGAGGAGTTTTCCTTGGAGTTTCTTCAGGATTGTGGTGCGGTGAGAAGAGTTCCTTCTGCACCTGATACAGTTTTGAATTGTGAAAAGGGGGCTGGATTGAATTATAATCAGAATTCTACAGTGGCATATCAGGATCTTAGTGGTATTTTGGGGTTGAGGAGAATGGAGTCTGAGTGTGCATCTGACACATCTGAGTTTGTTGCGGCAAATGGGTCATGTAAAGAGATTGAAAATGAGGCTCATGTTGATAAGCTAAGCAGACACAGCGTGAAGGAGGTTGATGGTCGAGAAGGATCAAGGAAGGCTGTTGGAGAACTAAATTTTGATCGAGCTGGTTCTCGGCCAACTGCTCCACCTGTGAACGTGTCTGAGTCTCCTCATTCTAACAACTTTAATCGGTCAGGAGTGTTGCATGGTTCTCAGTCTGGGAAGATGAAATTTCTTTGCAGTTTTGGCGGAAAGATTTTACCCAGGCCTAGTGATGGGAAACTCAGATATGTTGGGGGAGAGACGCGTTTTATTTCTATTAGGAATTGTGTTTCATGGGAAGAGCTTGTGAAGAAAACTTCCGGTTTTTGCAGCCAGCCTCATATGATAAAATACCAGCTTCCAAATGAGGATCTTGATGCTCTTATATCTGTGTCATCTGATGAAGATCTTCAAAATATGATAGAGGAGTATCATGGGCTTGAAAGGCATGAGAGTTCTCAGAGGCCACGGGTCTTTTTGATTCCTCTGGGTGAGTCTGAAAATTGTTCTATTGAGGTTGACACCATACATTCGAGTAACCCTGATTACCAATATGTTGCTGCTGTAAATGATTTGGTAGATCCAAGTTCTAGGAAAACTAGTGGAGGACAGAATTTGAGTAGTGAAGCAACTCAACAGGGAACAAAGACTTTGGTATTCCATAAGGAGATTCAGAGTAATTTTAAAGCCTTGCATCATAGTCAAATTTTGAGTGAGTCCCAGAATATGACTGGGTCTTCAATTCAATCACCTCTTTCTCCTATTCCACATCAACAAGCTGATGATAGGCAATGGGGCCAATCTCATGGAGGGCATTGTCACAACCACAATCACAGCAAAACTGTGGTGTCTACGTCAGCTGTTGGCCAAAACTTTGGTGACTTTGATGGGTTCTCCCACGAGATGCCAGTGCATAAGGAAAGTAGATTTCTTTCTGACAAGCCTGTCTCACAGCCAGAAGATCTAATAGCTCAGTTGTCAGGTTCCATTGGTTGTCATCATGGCATGCCACATGCACTTTCTGATTCAAAACTACAAGAGAATGGAGGGAGGTCTGCATACTGTTCTCAAGAAGGAATGAACTTATCACACTCCTTAAACTTTGCAAACGCTCAATTGTTATCATTCCAGAACTCAGGAATTGCACAAGAAAAGCCAACCCAACTGCATGAAAACAGTGACCCATTCAATCCTTGGGTACAAAACAAGTTAGAGGATATTGAGTCGGTAGGACTGCAGAGAAGAATAGATTTGCCAAATTCTTCTCCATGTTTAGAATCATTGGGCAGGAATGAACATACTCAGAACGGTTCTGGTGACGTTCATGACAAATTCCGAACAGCTATAAAGAAAGATTCTATGACTTTGGAACTCCCAAATAAGTTGAACGAAAAGGATATTTTCTTGCATCATGATGACACCCTTTATGGGGAAAGATCACCTGTCACTGGGGTGGAATGTCCAAAGAGATTGCCTAATATAAACCCCAATATAACATCTACTTTTGCCTCTGGGGTTGTGGTTCCTGCAGCCAGTGATTTCATACCTTTGGTAGATAATACAGTGCATGACCCCAAGAACTTCCAACTTGGCAAAACCCCTGCCAGTCTTCTTGTCACGAGTGAAGCAACTGCCAATGACCAGGATTGTACTTTGACAGGGATGGTAAATGTTGAACAAGGACACGCTGTTTCAGCGGCTAAGAATCCTGAAGTTTCAGGCCGATTTCCAAGCACTAGAGAACACTCCTGTGATGGACGTTCTTTGGGTGATCTAATCTCAGGGCTGTCCAATGGACCAATTTCCCATGAACCTGTACATCCACAATTTGTTGCAAGTCAACATGATATAGGTTTCCAAGAACCCCTGCTCACAAGCTCTGCAAATTTTTCCCCATCAACAGTTCTTGATGATCTTGAGTTCCAAAAGAGTGATCATATGGTGTTGCAGAAGCCAATCCAAAATGCTGCTTTTAAAAGAGAAGTATCCCTCCTTGATGATGATTTTGTGTGTTATCCTGCTACCAGTGTTGAGAATTTGAGCTTGAATGGCGAAAATGTCTCTTTGGCACAGACACAAGCCCAAACAATGAGTAATCATAAGGGACAGTTGGATACAGTGATAACTGCGGAAGATGTAAACAGTGGTGTTACTCCTGGCATTCAGTCTTTATTCGTGGTGTCCCCATATGTTGTTGACGAATCCATTGGTGATCTCATATCCCCAACTGCAACAGAGGTGGAGAGCATCATTCAAGAGTCTGAGTTTGAGGATGATAAGGGTGATATGGGAGACAAGAATGATTCATTTAGTGATGCAATGATAGTTGAAATGGAAGCCACCCTCTATGGTTTGCAGATCATTAGAAATGCTGATCTTGAAGAACTACGGGAATTGGGATCTGGTACATATGGAACTGTTTATCACGGAAAATGGCGGGGATCGGATGTTGCTATAAAGAGAATTAAAAAGGGCTGCTTTGCAGGGAGATCATCAGAGAAAAATCAATTGACAAAAGACTTTTGGAGAGAGGCACAGATCCTTTCAAATCTTCATCATCCAAATGTGGTTGCATTTTATGGGATAGTACCTGATGGAGCAGGAGGAACATTAGCAACTGTAGCTGAATATATGGTGAATGGATCCCTTAGGCATGCCCTACTTAAGAAGAATAGATCACTTGACCGGCGTAAAAAGCTTATAATTGCTATGGATGCAGCTTTTGGCATGGAATACTTGCATTCAAAGAATATTGTCCATTTTGATTTGAAGTGTGACAATTTGCTAGTCAACTTGAGGGATCCTCAACGACCTATATGTAAGGTAAATAGTACACAGACCTTATCCATGTTCAGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1085

Amino Acids

119.02

Weight (kDa)

5.44

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 210 - 292 2e-18 PB1 domain
Pkinase PF00069 926 - 1065 7.5e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 927 - 1064 4.2e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 252, 3098
AasI GACNNNNNNGTC 1 cut(s) 1340
Acc16I TGCGCA 1 cut(s) 122
Acc65I GGTACC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 32
AccB7I CCANNNNNTGG 1 cut(s) 106
AccI GTMKAC 2 cut(s) 86, 1247
AccII CGCG 1 cut(s) 661
AciI CCGC 6 cut(s) 185, 350, 603, 2093, 2525, 2833
AcoI YGGCCR 2 cut(s) 488, 1261
AcsI RAATTY 9 cut(s) 259, 466, 584, 1009, 1101, 1654, 1727, 2281, 2431
AcuI CTGAAG 2 cut(s) 155, 2127
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 7 cut(s) 34, 1596, 2047, 2214, 2803, 2985, 3229
AfiI CCNNNNNNNGG 5 cut(s) 106, 629, 1824, 1825, 1844
AflII CTTAAG 1 cut(s) 3059
AflIII ACRYGT 2 cut(s) 510, 659
AgeI ACCGGT 1 cut(s) 41
AhlI ACTAGT 1 cut(s) 995
AjnI CCWGG 3 cut(s) 617, 2037, 2551
AleI CACNNNNGTG 2 cut(s) 1847, 2145
Alw26I GTCTC 6 cut(s) 136, 525, 650, 1348, 2461, 2682
AoxI GGCC 6 cut(s) 488, 620, 860, 1192, 1261, 2116
ApeKI GCWGC 7 cut(s) 4, 726, 956, 1925, 2363, 2871, 3113
ApoI RAATTY 9 cut(s) 259, 466, 584, 1009, 1101, 1654, 1727, 2281, 2431
AsiGI ACCGGT 1 cut(s) 41
Asp700I GAANNNNTTC 1 cut(s) 804
Asp718I GGTACC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 1192, 2192
AvaII GGWCC 1 cut(s) 2192
AxyI CCTNAGG 1 cut(s) 3044
BalI TGGCCA 1 cut(s) 1263
BamHI GGATCC 3 cut(s) 72, 3038, 3196
BanI GGYRCC 1 cut(s) 32
BauI CACGAG 2 cut(s) 1295, 2014
BbsI GAAGAC 2 cut(s) 1125, 1997
BbvI GCAGC 7 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125
BccI CCATC 7 cut(s) 422, 623, 1277, 2053, 2144, 2299, 2984
BciT130I CCWGG 3 cut(s) 619, 2039, 2553
BciVI GTATCC 2 cut(s) 2392, 2503
BclI TGATCA 1 cut(s) 2329
BcoDI GTCTC 6 cut(s) 136, 525, 650, 1348, 2461, 2682
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
BfmI CTRYAG 4 cut(s) 264, 1631, 1923, 3015
BfrI CTTAAG 1 cut(s) 3059
BfuI GTATCC 2 cut(s) 2392, 2503
BglII AGATCT 2 cut(s) 802, 1357
BisI GCNGC 9 cut(s) 5, 351, 727, 957, 1926, 2094, 2364, 2872, 3114
BlpI GCTNAGC 1 cut(s) 401
BlsI GCNGC 9 cut(s) 6, 352, 728, 958, 1927, 2095, 2365, 2873, 3115
Bme1390I CCNGG 3 cut(s) 619, 2039, 2553
Bme18I GGWCC 1 cut(s) 2192
BmgT120I GGNCC 2 cut(s) 1192, 2192
BmiI GGNNCC 7 cut(s) 34, 74, 1193, 1381, 1920, 3040, 3198
BmrFI CCNGG 3 cut(s) 619, 2039, 2553
BmrI ACTGGG 3 cut(s) 86, 1137, 1854
BmsI GCATC 8 cut(s) 335, 763, 1099, 1290, 1813, 2652, 2701, 3100
BmuI ACTGGG 3 cut(s) 86, 1137, 1854
BpiI GAAGAC 2 cut(s) 1125, 1997
BplI GAGNNNNNCTC 2 cut(s) 3184, 3216
Bpu1102I GCTNAGC 1 cut(s) 401
BpuEI CTTGAG 3 cut(s) 1449, 2333, 3211
BsaBI GATNNNNATC 1 cut(s) 801
BsaJI CCNNGG 4 cut(s) 159, 617, 863, 1589
BsaWI WCCGGW 2 cut(s) 41, 716
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 629, 1824, 1825, 1844
Bse118I RCCGGY 2 cut(s) 41, 3081
Bse1I ACTGG 7 cut(s) 92, 1132, 1304, 1849, 1929, 2001, 2421
Bse21I CCTNAGG 1 cut(s) 3044
Bse3DI GCAATG 2 cut(s) 1193, 2721
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 3 cut(s) 619, 2039, 2553
BseDI CCNNGG 4 cut(s) 159, 617, 863, 1589
BseGI GGATG 6 cut(s) 2064, 2215, 2674, 2848, 2953, 3115
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 5 cut(s) 106, 629, 1824, 1825, 1844
BseMI GCAATG 2 cut(s) 1193, 2721
BseNI ACTGG 7 cut(s) 92, 1132, 1304, 1849, 1929, 2001, 2421
BseRI GAGGAG 4 cut(s) 164, 319, 513, 836
BseXI GCAGC 7 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125
BsgI GTGCAG 2 cut(s) 158, 189
Bsh1236I CGCG 1 cut(s) 661
BshFI GGCC 6 cut(s) 490, 622, 862, 1194, 1263, 2118
BshNI GGYRCC 1 cut(s) 32
BshTI ACCGGT 1 cut(s) 41
BsiSI CCGG 3 cut(s) 42, 717, 3082
BslFI GGGAC 3 cut(s) 1099, 2514, 2563
BslI CCNNNNNNNGG 5 cut(s) 106, 629, 1824, 1825, 1844
BsmAI GTCTC 6 cut(s) 136, 525, 650, 1348, 2461, 2682
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 3 cut(s) 1099, 2514, 2563
BsmI GAATGC 2 cut(s) 2556, 3136
BsnI GGCC 6 cut(s) 490, 622, 862, 1194, 1263, 2118
Bsp1407I TGTACA 1 cut(s) 2212
Bsp1720I GCTNAGC 1 cut(s) 401
BspACI CCGC 6 cut(s) 185, 350, 603, 2093, 2525, 2833
BspANI GGCC 6 cut(s) 490, 622, 862, 1194, 1263, 2118
BspFNI CGCG 1 cut(s) 661
BspHI TCATGA 2 cut(s) 1720, 1807
BspLI GGNNCC 7 cut(s) 34, 74, 1193, 1381, 1920, 3040, 3198
BspMAI CTGCAG 2 cut(s) 1635, 1927
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 3059
BsrDI GCAATG 2 cut(s) 1193, 2721
BsrFI RCCGGY 2 cut(s) 41, 3081
BsrGI TGTACA 1 cut(s) 2212
BsrI ACTGG 7 cut(s) 92, 1132, 1304, 1849, 1929, 2001, 2421
BssAI RCCGGY 2 cut(s) 41, 3081
BssECI CCNNGG 4 cut(s) 159, 617, 863, 1589
BssSI CACGAG 2 cut(s) 1295, 2014
BssT1I CCWWGG 2 cut(s) 159, 1589
Bst2BI CACGAG 2 cut(s) 1295, 2014
Bst2UI CCWGG 3 cut(s) 619, 2039, 2553
Bst6I CTCTTC 2 cut(s) 187, 690
BstAFI CTTAAG 1 cut(s) 3059
BstAUI TGTACA 1 cut(s) 2212
BstC8I GCNNGC 3 cut(s) 731, 1403, 3051
BstDSI CCRYGG 1 cut(s) 863
BstF5I GGATG 6 cut(s) 2064, 2215, 2674, 2848, 2953, 3115
BstFNI CGCG 1 cut(s) 661
BstHHI GCGC 1 cut(s) 123
BstMAI GTCTC 6 cut(s) 136, 525, 650, 1348, 2461, 2682
BstMWI GCNNNNNNNGC 8 cut(s) 411, 600, 841, 2090, 2445, 3017, 3091, 3110
BstNI CCWGG 3 cut(s) 619, 2039, 2553
BstNSI RCATGY 3 cut(s) 1405, 1412, 3053
BstSCI CCNGG 3 cut(s) 617, 2037, 2551
BstSFI CTRYAG 4 cut(s) 264, 1631, 1923, 3015
BstUI CGCG 1 cut(s) 661
BstV1I GCAGC 7 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125
BstV2I GAAGAC 2 cut(s) 1125, 1997
BstXI CCANNNNNNTGG 2 cut(s) 1202, 1271
Bsu36I CCTNAGG 1 cut(s) 3044
BsuI GTATCC 2 cut(s) 2392, 2503
BsuRI GGCC 6 cut(s) 490, 622, 862, 1194, 1263, 2118
BtgI CCRYGG 1 cut(s) 863
BtsCI GGATG 6 cut(s) 2064, 2215, 2674, 2848, 2953, 3115
Cac8I GCNNGC 3 cut(s) 731, 1403, 3051
CciI TCATGA 2 cut(s) 1720, 1807
CfoI GCGC 1 cut(s) 123
Cfr10I RCCGGY 2 cut(s) 41, 3081
Cfr13I GGNCC 2 cut(s) 1192, 2192
CseI GACGC 1 cut(s) 667
Csp6I GTAC 7 cut(s) 33, 1595, 2046, 2213, 2802, 2984, 3228
CspAI ACCGGT 1 cut(s) 41
CspCI CAANNNNNGTGG 2 cut(s) 2616, 2651
CviQI GTAC 7 cut(s) 33, 1595, 2046, 2213, 2802, 2984, 3228
DraI TTTAAA 2 cut(s) 1081, 2371
DraIII CACNNNGTG 1 cut(s) 415
DrdI GACNNNNNNGTC 1 cut(s) 1340
DseDI GACNNNNNNGTC 1 cut(s) 1340
EaeI YGGCCR 2 cut(s) 488, 1261
Eam1104I CTCTTC 2 cut(s) 187, 690
EarI CTCTTC 2 cut(s) 187, 690
EciI GGCGGA 1 cut(s) 618
Eco130I CCWWGG 2 cut(s) 159, 1589
Eco147I AGGCCT 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 2192
Eco57I CTGAAG 2 cut(s) 155, 2127
Eco81I CCTNAGG 1 cut(s) 3044
EcoRI GAATTC 1 cut(s) 259
EcoRII CCWGG 3 cut(s) 617, 2037, 2551
EcoT14I CCWWGG 2 cut(s) 159, 1589
ErhI CCWWGG 2 cut(s) 159, 1589
Esp3I CGTCTC 1 cut(s) 650
FalI AAGNNNNNCTT 4 cut(s) 184, 216, 2858, 2890
FaqI GGGAC 3 cut(s) 1099, 2514, 2563
FauI CCCGC 1 cut(s) 2826
FauNDI CATATG 4 cut(s) 738, 2334, 2584, 2806
FbaI TGATCA 1 cut(s) 2329
FblI GTMKAC 2 cut(s) 86, 1247
Fnu4HI GCNGC 9 cut(s) 5, 351, 727, 957, 1926, 2094, 2364, 2872, 3114
FokI GGATG 6 cut(s) 2071, 2202, 2681, 2855, 2940, 3122
Fsp4HI GCNGC 9 cut(s) 5, 351, 727, 957, 1926, 2094, 2364, 2872, 3114
FspBI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
FspI TGCGCA 1 cut(s) 122
GlaI GCGC 1 cut(s) 122
GluI GCNGC 9 cut(s) 5, 351, 727, 957, 1926, 2094, 2364, 2872, 3114
HaeIII GGCC 6 cut(s) 490, 622, 862, 1194, 1263, 2118
HapII CCGG 3 cut(s) 42, 717, 3082
HgaI GACGC 1 cut(s) 667
HhaI GCGC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 5 cut(s) 87, 916, 2237, 2593, 3187
HindII GTYRAC 5 cut(s) 87, 916, 2237, 2593, 3187
HindIII AAGCTT 1 cut(s) 3092
HpaII CCGG 3 cut(s) 42, 717, 3082
Hpy166II GTNNAC 9 cut(s) 87, 508, 916, 1248, 2237, 2536, 2593, 3187, 3230
Hpy8I GTNNAC 9 cut(s) 87, 508, 916, 1248, 2237, 2536, 2593, 3187, 3230
HpyAV CCTTC 5 cut(s) 210, 412, 431, 442, 1463
HpyCH4IV ACGT 4 cut(s) 510, 1250, 1716, 2155
HpyF10VI GCNNNNNNNGC 8 cut(s) 411, 600, 841, 2090, 2445, 3017, 3091, 3110
HpySE526I ACGT 4 cut(s) 510, 1250, 1716, 2155
HspAI GCGC 1 cut(s) 121
KpnI GGTACC 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 2329
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 2 cut(s) 502, 2993
Lsp1109I GCAGC 7 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125
LweI GCATC 8 cut(s) 335, 763, 1099, 1290, 1813, 2652, 2701, 3100
MaeI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
MaeII ACGT 4 cut(s) 510, 1250, 1716, 2155
MaeIII GTNAC 9 cut(s) 932, 1214, 1274, 1574, 1712, 1840, 2011, 2545, 3170
MfeI CAATTG 2 cut(s) 1511, 2903
MlsI TGGCCA 1 cut(s) 1263
MluI ACGCGT 1 cut(s) 659
MluNI TGGCCA 1 cut(s) 1263
MlyI GAGTC 7 cut(s) 92, 323, 527, 899, 1121, 1628, 2663
MmeI TCCRAC 3 cut(s) 436, 2005, 2487
Mox20I TGGCCA 1 cut(s) 1263
MroXI GAANNNNTTC 1 cut(s) 804
MscI TGGCCA 1 cut(s) 1263
MseI TTAA 6 cut(s) 540, 1080, 1493, 2370, 2862, 3060
MslI CAYNNNNRTG 4 cut(s) 1809, 1847, 2145, 2958
Msp20I TGGCCA 1 cut(s) 1263
MspA1I CMGCKG 2 cut(s) 1256, 2093
MspCI CTTAAG 1 cut(s) 3059
MspI CCGG 3 cut(s) 42, 717, 3082
MspR9I CCNGG 3 cut(s) 619, 2039, 2553
MunI CAATTG 2 cut(s) 1511, 2903
Mva1269I GAATGC 2 cut(s) 2556, 3136
MvaI CCWGG 3 cut(s) 619, 2039, 2553
MvnI CGCG 1 cut(s) 661
MwoI GCNNNNNNNGC 8 cut(s) 411, 600, 841, 2090, 2445, 3017, 3091, 3110
NdeI CATATG 4 cut(s) 738, 2334, 2584, 2806
NlaIV GGNNCC 7 cut(s) 34, 74, 1193, 1381, 1920, 3040, 3198
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 7 cut(s) 1214, 1274, 1574, 1712, 1840, 2011, 3170
NsbI TGCGCA 1 cut(s) 122
NspI RCATGY 3 cut(s) 1405, 1412, 3053
OliI CACNNNNGTG 2 cut(s) 1847, 2145
PaeI GCATGC 2 cut(s) 1405, 3053
PagI TCATGA 2 cut(s) 1720, 1807
PceI AGGCCT 1 cut(s) 622
PciSI GCTCTTC 1 cut(s) 690
PctI GAATGC 2 cut(s) 2556, 3136
PdmI GAANNNNTTC 1 cut(s) 804
PfeI GAWTC 8 cut(s) 877, 1066, 1421, 1673, 1751, 2101, 2597, 2699
PflFI GACNNNGTC 1 cut(s) 1129
PflMI CCANNNNNTGG 1 cut(s) 106
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 9 cut(s) 6, 352, 728, 958, 1927, 2095, 2365, 2873, 3115
PleI GAGTC 7 cut(s) 91, 322, 526, 898, 1120, 1627, 2662
PpsI GAGTC 7 cut(s) 91, 322, 526, 898, 1120, 1627, 2662
PsiI TTATAA 2 cut(s) 252, 3098
Psp6I CCWGG 3 cut(s) 617, 2037, 2551
PspGI CCWGG 3 cut(s) 617, 2037, 2551
PspN4I GGNNCC 7 cut(s) 34, 74, 1193, 1381, 1920, 3040, 3198
PspPI GGNCC 2 cut(s) 1192, 2192
PstI CTGCAG 2 cut(s) 1635, 1927
PsyI GACNNNGTC 1 cut(s) 1129
PvuII CAGCTG 1 cut(s) 1256
RsaI GTAC 7 cut(s) 34, 1596, 2047, 2214, 2803, 2985, 3229
RsaNI GTAC 7 cut(s) 33, 1595, 2046, 2213, 2802, 2984, 3228
RseI CAYNNNNRTG 4 cut(s) 1809, 1847, 2145, 2958
SalI GTCGAC 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 6 cut(s) 540, 1080, 1493, 2370, 2862, 3060
SatI GCNGC 9 cut(s) 5, 351, 727, 957, 1926, 2094, 2364, 2872, 3114
Sau96I GGNCC 2 cut(s) 1192, 2192
SchI GAGTC 7 cut(s) 92, 323, 527, 899, 1121, 1628, 2663
ScrFI CCNGG 3 cut(s) 619, 2039, 2553
SfaNI GCATC 8 cut(s) 335, 763, 1099, 1290, 1813, 2652, 2701, 3100
SfcI CTRYAG 4 cut(s) 264, 1631, 1923, 3015
SinI GGWCC 1 cut(s) 2192
SmiMI CAYNNNNRTG 4 cut(s) 1809, 1847, 2145, 2958
SmlI CTYRAG 4 cut(s) 1464, 2312, 3059, 3190
SmoI CTYRAG 4 cut(s) 1464, 2312, 3059, 3190
SpeI ACTAGT 1 cut(s) 995
SphI GCATGC 2 cut(s) 1405, 3053
SseBI AGGCCT 1 cut(s) 622
SsiI CCGC 6 cut(s) 185, 350, 603, 2093, 2525, 2833
SspI AATATT 1 cut(s) 3148
SspMI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 3 cut(s) 617, 2037, 2551
StyI CCWWGG 2 cut(s) 159, 1589
TaiI ACGT 4 cut(s) 513, 1253, 1719, 2158
TaqI TCGA 4 cut(s) 86, 433, 475, 929
TaqII GACCGA 1 cut(s) 534
TatI WGTACW 3 cut(s) 2045, 2212, 3227
TauI GCSGC 2 cut(s) 353, 2096
TfiI GAWTC 8 cut(s) 877, 1066, 1421, 1673, 1751, 2101, 2597, 2699
Tru1I TTAA 6 cut(s) 540, 1080, 1493, 2370, 2862, 3060
Tru9I TTAA 6 cut(s) 540, 1080, 1493, 2370, 2862, 3060
TseFI GTSAC 7 cut(s) 1214, 1274, 1574, 1712, 1840, 2011, 3170
TseI GCWGC 7 cut(s) 4, 726, 956, 1925, 2363, 2871, 3113
Tsp45I GTSAC 7 cut(s) 1214, 1274, 1574, 1712, 1840, 2011, 3170
TspGWI ACGGA 1 cut(s) 2838
Tth111I GACNNNGTC 1 cut(s) 1129
Van91I CCANNNNNTGG 1 cut(s) 106
Vha464I CTTAAG 1 cut(s) 3059
VpaK11BI GGWCC 1 cut(s) 2192
XapI RAATTY 9 cut(s) 259, 466, 584, 1009, 1101, 1654, 1727, 2281, 2431
XceI RCATGY 3 cut(s) 1405, 1412, 3053
XcmI CCANNNNNNNNNTGG 2 cut(s) 1124, 1587
XmiI GTMKAC 2 cut(s) 86, 1247
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.