Rmu_sc0006801.1_g000005

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006801.1
OG0009051
Physical Location & Seq
Reverse (-)
27804 .. 33913
6110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006801.1_g000005.1.cds

Sequence Viewer

Length: 3666 bp
atgaccggtgaggtgtcatgctcatcaggtcaatggatccagcaagagttgaccccagttgtccagaatatgggaaagaatgtgcgcagaaatgtctctgtacagacgggggaggagttttccttggagtttcttcaggattgtggtgcggtgagaagagttccttctgcacctgatacagttctgaattgtgacaagggggctggattgaattataatcagaattctacagtggcatatcaggatcttagtggtattttggggttgaggagaatggagtctgagcgtgcatctgacacatctgagtttgttgcggcaaatgggtcatgtaaagagattgaaaatgaggctcatgttgataagctaagcagacacagcgtgaaggaggttgatggtggagaaggatcaaggaaggctgttggagaactaaattgtgatcgagctggttttcggccaactgctccacctgtgaacgtgtctgagtctcctcattctaacaactttaatcggtcaggagtgttgcatggttctcagtctgggaagatgaaatttctttgcagttttggtggaaagattttacccaggcctagtgatgggaaactcagatatgttgggggagagacgcgttttatttctattaggaatggtgtttcatgggaagagcttgtgaagaaaacttccggtttttgcagccagcctcatatgataaaataccagcttccaaatgaggatcttgatgctcttatatctgtgtcatctgatgaagatcttcaaaatatgatagaggagtatcatgggcttgaaaggcatgacaattctcagaggccacgggtctttttgattcctctgggtgagtctgaaaattgttccattgaggttgacaccatacattcgagtaaccctgattaccaatatgttgctgctgtaaatgatttggtagatccaagttctaggaaaactagtggaggacagaatttgagtagtgaagcaagtcaacagggaacaaaggctttggtattccataaggagattcagagtaattttaaagccttgcatcatagtcaaattttgagtgattcccagaatatgactgggtcttcaattcaatcacctctttctcctattccacatcaacaagctgatgataggcaatggggccaatctcatggagggcattgtcacaaccacaatcacagcaaaactgtggtgtctacttcagctgttggccaaaacgttggtgactttgatgggttctcctgcgagatgccagtgcataaggaaagtagatttctttccgacaagcctgtctcacagccagaagatctaatagctcaattgtcaggttccattggttgtcatcatggcatgccacatgcactttctgattcaaaactacaagagaatggagggaggtctgcatactgttctcaagaaggaatgaacttatcacactccttaaactttgcaaacgctcaattgttatcattccagaactcaggaattgcacaagaaaagccaacccaactgcatgaaaacagtgaccctttcaatccttgggtacaaaacaagttagaggatattgagtcggtaggactgcagagaagactggatttgccaaattcttctccatgtttagaatcattgggcaggaatgaacatactcagaacggttctggtgacgttcatgacaaattccgaacagctataaagaaagattctatgactttggaactcccaaataagttgaacgaaaaggatattttcttgcatcatgatgacaccctttatggggaaagatcacctgtcactgggttggaatgtccgaagagattgcctaatataaaccccaatataacatctacttttgcctctggggttgtggttcctgcagccagtgatttcatacctttggtagataatacagtgcatgaccccaagaacttccaacttggcaaaacccctgccagtcttcttgtcacgagtgaagcaactgccaatgaccaggattgtgctttgacagggatggtaaatgttgaacaaggacacgctgtttcagtggctaagaatcctgaagtttcaggccgatttccaagcactagagaacactcctgtgatgggcgttctttgggtgatctaatttcagggttgtccaatggaccaatttcccatgaacctgtacatccacaatttgttgcaagccaacatgatataggtttccaagaacccctgctcacaagctctgcaaatttttgcccatcgacagttcttgatgatcttgagttccaaaagagtgatcatatggtgttgcagaagccaatccaaaatgctgctttcaaaagagaagtatccctccttgatgatgattttgtgtgttatcctgctaccagtgttgagaatttgagcttgaatggcgaaaatgtctctttggcacaaacacaagcccaaacaatgagtaatcataagggacagttggatacagtaataactgcggaagatgtaaacagcggcgttactcctggcattcagtctttattcgtggtgtccccatatgttgttgacgaatccattggtgatctcatatccccaactgcaacagaggtggagagcatcattcaagagtctgagtttgaggatgataagggtgatatgggagacaagaatgattcatttagtgatgcaatgatagttgaaatggaagccaccctctatggtttgcagatcattagaaatgctgatcttgaagaactacgggaattgggatctggtacatatggaactgtttatcacggaaaatggcggggatcggatgttgctataaagagaattaaaaagggctgctttgcagggagatcatcagggaaagatcaattgacaaaagacttttggagagaggcacagatcctttcaaatcttcatcatccaaatgtggttgcattttatgggatagtacctgatgcagcaggaggaacattagcaactgtagctgaatatatggtgaatggatcccttaggcatgccctacttaagaagaatagatcacttgaccggcgtaaaaagcttataattgctatggatgcagcttttggcatggaatacttgcattcaaagaatattgtccattttgatttgaagtgtgacaatttgctagtcaacttgagggatcctcaacgacctatatgtaaggttggagattttggattatcaagaatcaaacgcaatactttggtatccggtggtgtgcgaggaacacttccatggatggcaccagaattgttgaacggtagcagcagccgggtctctgagaaggttgatgttttctcatttggaatttcaatgtgggagatcttgactggagaggaaccgtatgcaaatatgcattgtggtgccatcattgggggaattgtaaagaacactcttcgaccacctattccagagcgatgtgattctgagtggaggacgctaatggaagagtgctggtcacctgatccagaaagtcggccgtcatttactgagataactaacaagttgcgagctatgtctaacacacttcagggaaaggcttctgttgacattcttcaggtacgaggatcatctagtattgctccaatgaaaacccttctctttgagtacgaaaactttgagggttggtttgtccccaacatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1221

Amino Acids

133.95

Weight (kDa)

5.42

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 216, 3062
AasI GACNNNNNNGTC 1 cut(s) 1304
Acc16I TGCGCA 1 cut(s) 86
AccB1I GGYRCC 2 cut(s) 3262, 3383
AccB7I CCANNNNNTGG 1 cut(s) 70
AccI GTMKAC 1 cut(s) 1211
AccII CGCG 1 cut(s) 625
AciI CCGC 5 cut(s) 149, 314, 2489, 2505, 2797
AclI AACGTT 1 cut(s) 1233
AcoI YGGCCR 3 cut(s) 452, 1225, 3497
AcsI RAATTY 9 cut(s) 223, 548, 973, 1065, 1618, 1691, 2245, 2395, 3327
AcuI CTGAAG 5 cut(s) 119, 1200, 2091, 3533, 3560
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 7 cut(s) 102, 1560, 2178, 2767, 2949, 3582, 3629
AfiI CCNNNNNNNGG 7 cut(s) 70, 593, 1788, 1789, 1808, 2115, 3393
AflII CTTAAG 1 cut(s) 3023
AflIII ACRYGT 2 cut(s) 474, 623
AgeI ACCGGT 1 cut(s) 5
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 3 cut(s) 581, 2001, 2515
AleI CACNNNNGTG 1 cut(s) 2109
Alw26I GTCTC 7 cut(s) 100, 489, 614, 1312, 2425, 2646, 3301
AoxI GGCC 7 cut(s) 452, 584, 824, 1156, 1225, 2080, 3497
ApeKI GCWGC 9 cut(s) 690, 920, 1889, 2327, 2835, 2957, 3077, 3285, 3288
ApoI RAATTY 9 cut(s) 223, 548, 973, 1065, 1618, 1691, 2245, 2395, 3327
AsiGI ACCGGT 1 cut(s) 5
Asp700I GAANNNNTTC 2 cut(s) 768, 3559
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 2 cut(s) 1156, 2156
AsuC2I CCSGG 1 cut(s) 3293
AvaII GGWCC 1 cut(s) 2156
AxyI CCTNAGG 1 cut(s) 3008
BaeI ACNNNNGTAYC 2 cut(s) 3210, 3243
BalI TGGCCA 1 cut(s) 1227
BamHI GGATCC 3 cut(s) 36, 3002, 3160
BanI GGYRCC 2 cut(s) 3262, 3383
BauI CACGAG 1 cut(s) 1978
BbsI GAAGAC 3 cut(s) 1089, 1609, 1961
BbvI GCAGC 9 cut(s) 702, 907, 1901, 2314, 2822, 2969, 3089, 3297, 3300
BccI CCATC 8 cut(s) 386, 587, 1241, 2017, 2108, 2263, 3253, 3395
BceAI ACGGC 1 cut(s) 3484
BcgI CGANNNNNNTGC 2 cut(s) 1813, 1847
BciT130I CCWGG 3 cut(s) 583, 2003, 2517
BciVI GTATCC 3 cut(s) 2356, 2467, 3238
BclI TGATCA 1 cut(s) 2293
BcnI CCSGG 1 cut(s) 3293
BcoDI GTCTC 7 cut(s) 100, 489, 614, 1312, 2425, 2646, 3301
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 6 cut(s) 588, 951, 960, 2097, 3146, 3594
BfmI CTRYAG 4 cut(s) 228, 1595, 1887, 2979
BfrI CTTAAG 1 cut(s) 3023
BfuI GTATCC 3 cut(s) 2356, 2467, 3238
BglII AGATCT 3 cut(s) 766, 1321, 3342
BlpI GCTNAGC 1 cut(s) 365
Bme1390I CCNGG 4 cut(s) 583, 2003, 2517, 3293
Bme18I GGWCC 1 cut(s) 2156
BmgT120I GGNCC 2 cut(s) 1156, 2156
BmiI GGNNCC 9 cut(s) 38, 1157, 1345, 1884, 3004, 3162, 3264, 3360, 3385
BmrFI CCNGG 4 cut(s) 583, 2003, 2517, 3293
BmrI ACTGGG 3 cut(s) 50, 1101, 1818
BmsI GCATC 9 cut(s) 299, 727, 1063, 1254, 1777, 2616, 2665, 2944, 3064
BmuI ACTGGG 3 cut(s) 50, 1101, 1818
BpiI GAAGAC 3 cut(s) 1089, 1609, 1961
BplI GAGNNNNNCTC 2 cut(s) 3148, 3180
BpmI CTGGAG 1 cut(s) 3372
Bpu1102I GCTNAGC 1 cut(s) 365
BpuEI CTTGAG 3 cut(s) 1413, 2297, 3175
BpuMI CCSGG 1 cut(s) 3293
BsaBI GATNNNNATC 1 cut(s) 765
BsaI GGTCTC 1 cut(s) 3301
BsaJI CCNNGG 5 cut(s) 123, 581, 827, 1553, 3254
BsaWI WCCGGW 3 cut(s) 5, 680, 3230
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 593, 1788, 1789, 1808, 2115, 3393
Bse118I RCCGGY 2 cut(s) 5, 3045
Bse1I ACTGG 9 cut(s) 56, 1096, 1268, 1611, 1813, 1893, 1965, 2385, 3355
Bse21I CCTNAGG 1 cut(s) 3008
Bse3DI GCAATG 2 cut(s) 1157, 2685
Bse8I GATNNNNATC 1 cut(s) 765
BseBI CCWGG 3 cut(s) 583, 2003, 2517
BseDI CCNNGG 5 cut(s) 123, 581, 827, 1553, 3254
BseGI GGATG 7 cut(s) 2028, 2179, 2638, 2812, 2917, 3079, 3264
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 7 cut(s) 70, 593, 1788, 1789, 1808, 2115, 3393
BseMI GCAATG 2 cut(s) 1157, 2685
BseNI ACTGG 9 cut(s) 56, 1096, 1268, 1611, 1813, 1893, 1965, 2385, 3355
BseRI GAGGAG 4 cut(s) 128, 283, 477, 800
BseX3I CGGCCG 1 cut(s) 3497
BseXI GCAGC 9 cut(s) 702, 907, 1901, 2314, 2822, 2969, 3089, 3297, 3300
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 625
Bsh1285I CGRYCG 1 cut(s) 3500
BshFI GGCC 7 cut(s) 454, 586, 826, 1158, 1227, 2082, 3499
BshNI GGYRCC 2 cut(s) 3262, 3383
BshTI ACCGGT 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 3500
BsiSI CCGG 5 cut(s) 6, 681, 3046, 3231, 3292
BslFI GGGAC 3 cut(s) 2478, 2527, 3638
BslI CCNNNNNNNGG 7 cut(s) 70, 593, 1788, 1789, 1808, 2115, 3393
BsmAI GTCTC 7 cut(s) 100, 489, 614, 1312, 2425, 2646, 3301
BsmBI CGTCTC 1 cut(s) 614
BsmFI GGGAC 3 cut(s) 2478, 2527, 3638
BsmI GAATGC 2 cut(s) 2520, 3100
BsnI GGCC 7 cut(s) 454, 586, 826, 1158, 1227, 2082, 3499
Bso31I GGTCTC 1 cut(s) 3301
Bsp1407I TGTACA 2 cut(s) 100, 2176
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 3254
BspACI CCGC 5 cut(s) 149, 314, 2489, 2505, 2797
BspANI GGCC 7 cut(s) 454, 586, 826, 1158, 1227, 2082, 3499
BspFNI CGCG 1 cut(s) 625
BspHI TCATGA 2 cut(s) 1684, 1771
BspLI GGNNCC 9 cut(s) 38, 1157, 1345, 1884, 3004, 3162, 3264, 3360, 3385
BspMAI CTGCAG 2 cut(s) 1599, 1891
BspQI GCTCTTC 1 cut(s) 654
BspT107I GGYRCC 2 cut(s) 3262, 3383
BspTI CTTAAG 1 cut(s) 3023
BspTNI GGTCTC 1 cut(s) 3301
BsrDI GCAATG 2 cut(s) 1157, 2685
BsrFI RCCGGY 2 cut(s) 5, 3045
BsrGI TGTACA 2 cut(s) 100, 2176
BsrI ACTGG 9 cut(s) 56, 1096, 1268, 1611, 1813, 1893, 1965, 2385, 3355
BssAI RCCGGY 2 cut(s) 5, 3045
BssECI CCNNGG 5 cut(s) 123, 581, 827, 1553, 3254
BssSI CACGAG 1 cut(s) 1978
BssT1I CCWWGG 3 cut(s) 123, 1553, 3254
Bst2BI CACGAG 1 cut(s) 1978
Bst2UI CCWGG 3 cut(s) 583, 2003, 2517
Bst6I CTCTTC 5 cut(s) 151, 654, 1820, 3420, 3462
BstAFI CTTAAG 1 cut(s) 3023
BstAUI TGTACA 2 cut(s) 100, 2176
BstC8I GCNNGC 6 cut(s) 288, 695, 1367, 2197, 3015, 3531
BstDSI CCRYGG 2 cut(s) 827, 3254
BstEII GGTNACC 1 cut(s) 3477
BstF5I GGATG 7 cut(s) 2028, 2179, 2638, 2812, 2917, 3079, 3264
BstFNI CGCG 1 cut(s) 625
BstHHI GCGC 1 cut(s) 87
BstMAI GTCTC 7 cut(s) 100, 489, 614, 1312, 2425, 2646, 3301
BstMCI CGRYCG 1 cut(s) 3500
BstMWI GCNNNNNNNGC 6 cut(s) 375, 805, 2409, 2981, 3055, 3074
BstNI CCWGG 3 cut(s) 583, 2003, 2517
BstNSI RCATGY 3 cut(s) 1369, 1376, 3017
BstPI GGTNACC 1 cut(s) 3477
BstSCI CCNGG 4 cut(s) 581, 2001, 2515, 3291
BstSFI CTRYAG 4 cut(s) 228, 1595, 1887, 2979
BstUI CGCG 1 cut(s) 625
BstV1I GCAGC 9 cut(s) 702, 907, 1901, 2314, 2822, 2969, 3089, 3297, 3300
BstV2I GAAGAC 3 cut(s) 1089, 1609, 1961
BstXI CCANNNNNNTGG 2 cut(s) 1166, 1235
BstZI CGGCCG 1 cut(s) 3497
Bsu36I CCTNAGG 1 cut(s) 3008
BsuI GTATCC 3 cut(s) 2356, 2467, 3238
BsuRI GGCC 7 cut(s) 454, 586, 826, 1158, 1227, 2082, 3499
BtgI CCRYGG 2 cut(s) 827, 3254
BtgZI GCGATG 1 cut(s) 3451
BtsCI GGATG 7 cut(s) 2028, 2179, 2638, 2812, 2917, 3079, 3264
BtsIMutI CAGTG 8 cut(s) 237, 1275, 1543, 1806, 1900, 1929, 2061, 2392
Cac8I GCNNGC 6 cut(s) 288, 695, 1367, 2197, 3015, 3531
CciI TCATGA 2 cut(s) 1684, 1771
CfoI GCGC 1 cut(s) 87
Cfr10I RCCGGY 2 cut(s) 5, 3045
Cfr13I GGNCC 2 cut(s) 1156, 2156
CseI GACGC 2 cut(s) 631, 3466
Csp6I GTAC 7 cut(s) 101, 1559, 2177, 2766, 2948, 3581, 3628
CspAI ACCGGT 1 cut(s) 5
CspCI CAANNNNNGTGG 2 cut(s) 2580, 2615
CviQI GTAC 7 cut(s) 101, 1559, 2177, 2766, 2948, 3581, 3628
DraI TTTAAA 1 cut(s) 1045
DraIII CACNNNGTG 1 cut(s) 379
DrdI GACNNNNNNGTC 1 cut(s) 1304
DseDI GACNNNNNNGTC 1 cut(s) 1304
EaeI YGGCCR 3 cut(s) 452, 1225, 3497
EagI CGGCCG 1 cut(s) 3497
Eam1104I CTCTTC 5 cut(s) 151, 654, 1820, 3420, 3462
EarI CTCTTC 5 cut(s) 151, 654, 1820, 3420, 3462
EclXI CGGCCG 1 cut(s) 3497
Eco130I CCWWGG 3 cut(s) 123, 1553, 3254
Eco147I AGGCCT 1 cut(s) 586
Eco31I GGTCTC 1 cut(s) 3301
Eco47I GGWCC 1 cut(s) 2156
Eco52I CGGCCG 1 cut(s) 3497
Eco57I CTGAAG 5 cut(s) 119, 1200, 2091, 3533, 3560
Eco81I CCTNAGG 1 cut(s) 3008
Eco91I GGTNACC 1 cut(s) 3477
EcoO65I GGTNACC 1 cut(s) 3477
EcoRI GAATTC 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 581, 2001, 2515
EcoT14I CCWWGG 3 cut(s) 123, 1553, 3254
EcoT22I ATGCAT 1 cut(s) 3378
ErhI CCWWGG 3 cut(s) 123, 1553, 3254
Esp3I CGTCTC 1 cut(s) 614
FalI AAGNNNNNCTT 4 cut(s) 148, 180, 2822, 2854
FaqI GGGAC 3 cut(s) 2478, 2527, 3638
FauI CCCGC 1 cut(s) 2790
FauNDI CATATG 4 cut(s) 702, 2298, 2548, 2770
FbaI TGATCA 1 cut(s) 2293
FblI GTMKAC 1 cut(s) 1211
FokI GGATG 7 cut(s) 2035, 2166, 2645, 2819, 2904, 3086, 3271
FspBI CTAG 6 cut(s) 588, 951, 960, 2097, 3146, 3594
FspI TGCGCA 1 cut(s) 86
GlaI GCGC 1 cut(s) 86
GsuI CTGGAG 1 cut(s) 3372
HaeIII GGCC 7 cut(s) 454, 586, 826, 1158, 1227, 2082, 3499
HapII CCGG 5 cut(s) 6, 681, 3046, 3231, 3292
HgaI GACGC 2 cut(s) 631, 3466
HhaI GCGC 1 cut(s) 87
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 6 cut(s) 51, 880, 995, 2557, 3151, 3568
HindII GTYRAC 6 cut(s) 51, 880, 995, 2557, 3151, 3568
HindIII AAGCTT 1 cut(s) 3056
HpaII CCGG 5 cut(s) 6, 681, 3046, 3231, 3292
Hpy166II GTNNAC 9 cut(s) 51, 472, 880, 995, 1212, 2500, 2557, 3151, 3568
Hpy8I GTNNAC 9 cut(s) 51, 472, 880, 995, 1212, 2500, 2557, 3151, 3568
HpyAV CCTTC 7 cut(s) 174, 376, 395, 406, 1427, 3298, 3626
HpyCH4IV ACGT 3 cut(s) 474, 1233, 1680
HpyF10VI GCNNNNNNNGC 6 cut(s) 375, 805, 2409, 2981, 3055, 3074
HpySE526I ACGT 3 cut(s) 474, 1233, 1680
HspAI GCGC 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 2293
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 2 cut(s) 466, 3607
Lsp1109I GCAGC 9 cut(s) 702, 907, 1901, 2314, 2822, 2969, 3089, 3297, 3300
LweI GCATC 9 cut(s) 299, 727, 1063, 1254, 1777, 2616, 2665, 2944, 3064
MaeI CTAG 6 cut(s) 588, 951, 960, 2097, 3146, 3594
MaeII ACGT 3 cut(s) 474, 1233, 1680
MfeI CAATTG 3 cut(s) 1334, 1475, 2867
MlsI TGGCCA 1 cut(s) 1227
MluI ACGCGT 1 cut(s) 623
MluNI TGGCCA 1 cut(s) 1227
MlyI GAGTC 5 cut(s) 287, 491, 863, 1592, 2627
MmeI TCCRAC 6 cut(s) 400, 1320, 1794, 1969, 2451, 3166
Mox20I TGGCCA 1 cut(s) 1227
Mph1103I ATGCAT 1 cut(s) 3378
MroXI GAANNNNTTC 2 cut(s) 768, 3559
MscI TGGCCA 1 cut(s) 1227
MseI TTAA 5 cut(s) 504, 1044, 1457, 2826, 3024
MslI CAYNNNNRTG 5 cut(s) 1773, 2109, 2922, 3253, 3381
Msp20I TGGCCA 1 cut(s) 1227
MspA1I CMGCKG 2 cut(s) 1220, 2505
MspCI CTTAAG 1 cut(s) 3023
MspI CCGG 5 cut(s) 6, 681, 3046, 3231, 3292
MspR9I CCNGG 4 cut(s) 583, 2003, 2517, 3293
MunI CAATTG 3 cut(s) 1334, 1475, 2867
Mva1269I GAATGC 2 cut(s) 2520, 3100
MvaI CCWGG 3 cut(s) 583, 2003, 2517
MvnI CGCG 1 cut(s) 625
MwoI GCNNNNNNNGC 6 cut(s) 375, 805, 2409, 2981, 3055, 3074
NciI CCSGG 1 cut(s) 3293
NcoI CCATGG 1 cut(s) 3254
NdeI CATATG 4 cut(s) 702, 2298, 2548, 2770
NlaIV GGNNCC 9 cut(s) 38, 1157, 1345, 1884, 3004, 3162, 3264, 3360, 3385
NmuCI GTSAC 9 cut(s) 191, 1178, 1238, 1538, 1676, 1804, 1975, 3134, 3477
NsbI TGCGCA 1 cut(s) 86
NsiI ATGCAT 1 cut(s) 3378
NspI RCATGY 3 cut(s) 1369, 1376, 3017
OliI CACNNNNGTG 1 cut(s) 2109
PaeI GCATGC 2 cut(s) 1369, 3017
PagI TCATGA 2 cut(s) 1684, 1771
PceI AGGCCT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 654
PctI GAATGC 2 cut(s) 2520, 3100
PdmI GAANNNNTTC 2 cut(s) 768, 3559
PflFI GACNNNGTC 1 cut(s) 1093
PflMI CCANNNNNTGG 1 cut(s) 70
PinAI ACCGGT 1 cut(s) 5
PleI GAGTC 5 cut(s) 286, 490, 862, 1591, 2626
PpsI GAGTC 5 cut(s) 286, 490, 862, 1591, 2626
PsiI TTATAA 2 cut(s) 216, 3062
Psp1406I AACGTT 1 cut(s) 1233
Psp6I CCWGG 3 cut(s) 581, 2001, 2515
PspEI GGTNACC 1 cut(s) 3477
PspGI CCWGG 3 cut(s) 581, 2001, 2515
PspN4I GGNNCC 9 cut(s) 38, 1157, 1345, 1884, 3004, 3162, 3264, 3360, 3385
PspPI GGNCC 2 cut(s) 1156, 2156
PstI CTGCAG 2 cut(s) 1599, 1891
PsyI GACNNNGTC 1 cut(s) 1093
PvuII CAGCTG 1 cut(s) 1220
RsaI GTAC 7 cut(s) 102, 1560, 2178, 2767, 2949, 3582, 3629
RsaNI GTAC 7 cut(s) 101, 1559, 2177, 2766, 2948, 3581, 3628
RseI CAYNNNNRTG 5 cut(s) 1773, 2109, 2922, 3253, 3381
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 5 cut(s) 504, 1044, 1457, 2826, 3024
Sau96I GGNCC 2 cut(s) 1156, 2156
SchI GAGTC 5 cut(s) 287, 491, 863, 1592, 2627
ScrFI CCNGG 4 cut(s) 583, 2003, 2517, 3293
SfaNI GCATC 9 cut(s) 299, 727, 1063, 1254, 1777, 2616, 2665, 2944, 3064
SfcI CTRYAG 4 cut(s) 228, 1595, 1887, 2979
SinI GGWCC 1 cut(s) 2156
SmiMI CAYNNNNRTG 5 cut(s) 1773, 2109, 2922, 3253, 3381
SmlI CTYRAG 4 cut(s) 1428, 2276, 3023, 3154
SmoI CTYRAG 4 cut(s) 1428, 2276, 3023, 3154
SpeI ACTAGT 1 cut(s) 959
SphI GCATGC 2 cut(s) 1369, 3017
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 5 cut(s) 149, 314, 2489, 2505, 2797
SspI AATATT 1 cut(s) 3112
SspMI CTAG 6 cut(s) 588, 951, 960, 2097, 3146, 3594
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 4 cut(s) 581, 2001, 2515, 3291
StyI CCWWGG 3 cut(s) 123, 1553, 3254
TaiI ACGT 3 cut(s) 477, 1236, 1683
TaqI TCGA 4 cut(s) 439, 893, 2258, 3418
TaqII GACCGA 1 cut(s) 498
TatI WGTACW 2 cut(s) 100, 2176
TauI GCSGC 2 cut(s) 317, 2508
Tru1I TTAA 5 cut(s) 504, 1044, 1457, 2826, 3024
Tru9I TTAA 5 cut(s) 504, 1044, 1457, 2826, 3024
TscAI CASTG 8 cut(s) 237, 1275, 1543, 1813, 1900, 1929, 2061, 2392
TseFI GTSAC 9 cut(s) 191, 1178, 1238, 1538, 1676, 1804, 1975, 3134, 3477
TseI GCWGC 9 cut(s) 690, 920, 1889, 2327, 2835, 2957, 3077, 3285, 3288
Tsp45I GTSAC 9 cut(s) 191, 1178, 1238, 1538, 1676, 1804, 1975, 3134, 3477
TspGWI ACGGA 1 cut(s) 2802
TspRI CASTG 8 cut(s) 237, 1275, 1543, 1813, 1900, 1929, 2061, 2392
Tth111I GACNNNGTC 1 cut(s) 1093
Van91I CCANNNNNTGG 1 cut(s) 70
Vha464I CTTAAG 1 cut(s) 3023
VpaK11BI GGWCC 1 cut(s) 2156
XapI RAATTY 9 cut(s) 223, 548, 973, 1065, 1618, 1691, 2245, 2395, 3327
XceI RCATGY 3 cut(s) 1369, 1376, 3017
XcmI CCANNNNNNNNNTGG 1 cut(s) 1088
XmiI GTMKAC 1 cut(s) 1211
XmnI GAANNNNTTC 2 cut(s) 768, 3559
XspI CTAG 6 cut(s) 588, 951, 960, 2097, 3146, 3594
Zsp2I ATGCAT 1 cut(s) 3378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.