Rw0G000360

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00009
OG0009051
Physical Location & Seq
Forward (+)
12916 .. 19559
6644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G000360.1

Sequence Viewer

Length: 3444 bp
ATGAAAGCTCTGGACCTCCAGAACCCACCTCCAGGTACTCTCAATTTTCACTTTTTCAATTTTGATCGACGCCCATATGAGTTGTGTGTGCTTCGTTTAGCTCTGGGGGCTGGATTGAATTATAATCAGAATTCTACAGTGGCATATCAGGATCTTAGTGGTATTTTGGGGTTGAGGAGAATGGAGTCTGAGCGTGCATCTGACACATCTGAGTTTGTTGCGGCAAATGGGTCATGTAAAGAGATTGAAAATGAGGCTCATGTTGATAAGCTAAGCAGACACAGAGTGAAGGAGGTTGATGGTGGAGAAGGATCAAGGAAGGCTGTTGGAGAACTAAATTGTGATCGAGCTGGTTTTCGGCCAACTGCTCCACCTGTGAACGTGTCTGAGTCTCCTCATTCTAACAACTTTAATCGGTCAGGAGTGTTGCATGGTTCTCAGTCTGGGAAGATGAAATTTCTTTGCAGTTTTGGTGGAAAGATTTTACCCAGGCCTAGTGATGGGAAACTCAGATATGTTGGGGGAGAGACGCGTTTTATTTCTATTAGGAATGGTGTTTCATGGGAAGAGCTTGTGAAGAAAACTTCCGGTTTTTGCAGCCAGCCTCATATGATAAAATACCAGCTTCCAAATGAGGATCTTGATGCTCTTATATCTGTGTCATCTGATGAAGATCTTCAAAATATGATAGAGGAGCCACGGGTCTTTTTGATTCCTCTGGGTGAGTCTGAAAATTGTTCCATTGAGGTTGACACCATACATTCGAGTAACCCTGATTACCAATATGTTGCTGCTGTAAATGATTTGGTAGATCCAAGTTCTAGGAAAACTAGTGGAGGACAGAATTTGAGTAGTGAAGCAAGTCAACAGGGAACAAAGGCTTTGGTATTCCATAAGGAGATTCAGAGTAATTTTAAAGCCTTGCATCATAGTCAAATTTTGAGTGATTCCCTGAATATGACTGGGTCTTCAATTCAATCACCTCTTTCTCCTATTCCACATCAACAAGCTGATGATAGGCAATGGGGCCAATCTCATGGAGGGCATTGTCACAACCACAATCACAGCAAAACTGTGGTGTCTACTTCAGCTGTTGGCCAAAACGTTGGTGACTTTGATGGGTTCTCCTGCGAGATGCCAGTGCATAAGGAAAGTAGATTTCTTTCCGACAAGCCTGTCTCACAGCCAGAAGATCTAATAGCTCAGTTGTCAGGTTCCATTGGTTGTCATCATGGCATGCCACATGCACTTTCTGATTCAAAACTACAAGAGAATGGAGGGAGTTCTGCATACTGTTCTCAAGAAGGAATGAACTTATCACACTCCTTAAACTTTGCAAACGCTCAATTGTTATCATTCCAGAACTCAGGAATTGCACAAGAAAAGCCAACCCAACTGCATGAAAACAGTGACCCTTTCAATCCTTGGGTACAAAACAAGTTAGAGGATATTGAGTCGGTAGGACTGCAGAGAAGACTGGATTTGCCAAATTCTTCTCCATGTTTAGAATCATTGGGCAGGAATGAACATACTCAGAATGGTTCTGGTGACGTTCATGACAAATTCAGAACAGCTATAAAGAAAGATTCTATGACTTTGGAACTCCCAAATAAGTTGAACGAAAAGGATATTTTCTTGCATCATGATGACACCCTTTATGGGGAAAGATCACCTGTCACTGGGTTGGAATGTCCGAAGAGATTGCCGAATATAAACCCCAATATAACATCTACTTTTGCCTCTGGGGGTGTGGTTCCTGCAGCCAGTGATTTCATACCTTTGGTAGATAAAACAGTGCATGACCCCAAGAACTTCCAACTTGGCAAAACCCCTGCCAGTCTTCTTGTCACGAGTGAAGCAACTGCCAATGACCAGGATTGTGCTTTGACAGGGATGGTAAATGTTGAACAAGGACACGCTGTTTCAGCGGCTAAGAATCCTGAAGTTTCAGGCCGATTTCCAAGCACTAGAGAACACTCCTGTGATGGACGTTCTTTGGGTGATCTAATCTCAGGGTTGTCCAATGGACCAATTTCCCATGAACCTGTACATCCACAATTTGTTGCAAGTCAACATGATATAGGTTTCCAAGAACCCCTGCTCACAAGCTCTGCAAATTTTTACCCATCGACAGTTCTTGATGATCTTGAGTTCCAAAAGAGTGATCATATGGTGTTGCAGAAGCCAATCCAAAATGCTGCTTTTAAAAGAGAAGTATCCCTCCTTGATGATGATTTTGTGTGTTATCCTGCTACCAGTGTTGAGAATTTGAGCTTGAATGGCGAAAATGTCTCTTTGGCACAAACACAAGCCCAAACAATGAGTAATCATAAGGGACAGTTGGATACAGTAATAACTGCGGAAGATGTAAACAGTGGTGTTACTCCTGGCATTCAGTCTTTATTCGTGGTGTCCCCATATGTTGTTGACGAATCCATTGGTGATCTCATATCCCCAACTGCAACAGAGGTGGAGAGCATCATTCAAGAGTCTGAGTTTGAGGATGATAAGGGTGATATGGGAGACAAGAATGATTCATTTAGTGATGCAATGATAGTTGAAATGGAAGCCACCCTCTATGGTTTGCAGATCATTAGAAATGCTGATCTTGAAGAACTACGGGAATTGGGATCTGGTACATATGGAACTGTTTATCACGGAAAATGGCGGGGATCGGATGTTGCTATAAAGAGAATTAAAAAGGGCTGCTTTGCAGGGAGATCATCAGGGAAAGATCAATTGACAAAAGACTTTTGGAGAGAGGCACAGATCCTTTCAAATCTTCATCATCCAAATGTGGTTGCATTTTATGGGATAGTACCTGATGCAGCAGGAGGAACATTAGCAACTGTAGCTGAATATATGGTGAATGGATCCCTTAGGCATGCCCTACTTAAGAAGAATAGATCACTTGACCGGCGTAAAAAGCTTATAATTGCTATGGATGCAGCTTTTGGCATGGAATACTTGCATTCAAAGAATATTGTCCATTTTGATTTGAAGTGTGACAATTTGCTAGTCAACTTGAGGGATCCTCAACGGCCTATATGTAAGGTTGGAGATTTTGGATTATCAAGAATCAAACGCAATACTTTGGTATCCGGTGGTGTGCGAGGAACACTTCCATGGATGGCACCAGAATTGTTGAACGGTAGCAGCAGCCGGGTCTCTGAGAAGGTTGATGTTTTCTCATTTGGAATTTCAATGTGGGAGATCTTGACTGGAGAGGAACCGTATGCAAATATGCATTGTGGTGCCATCATTGGGGGAATTGTAAAGAACACTCTTCGACCACCTATTCCAGAGCGATGTGATTCTGAGTGGAGGACGCTAATGGAAGAGTGCTGGTCACCTGATCCAGAAAGTCGGCCGTCATTTACTGAGATAACTAACAAGTTGCGAGCTATGTCTAACACACTTCAGGGAAAGGCTTCTGGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1147

Amino Acids

125.7

Weight (kDa)

5.54

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 167 - 234 3e-17 PB1 domain
Pkinase PF00069 871 - 1131 1.4e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 872 - 1133 1.7e-63 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 123, 2933
AasI GACNNNNNNGTC 1 cut(s) 1175
AccB1I GGYRCC 2 cut(s) 3133, 3254
AccI GTMKAC 1 cut(s) 1082
AccII CGCG 1 cut(s) 532
AciI CCGC 4 cut(s) 221, 1928, 2360, 2668
AclI AACGTT 1 cut(s) 1104
AcoI YGGCCR 3 cut(s) 359, 1096, 3368
AcsI RAATTY 9 cut(s) 130, 455, 844, 936, 1489, 1562, 2116, 2266, 3198
AcuI CTGAAG 3 cut(s) 1071, 1962, 3404
AcyI GRCGYC 1 cut(s) 70
AdeI CACNNNGTG 1 cut(s) 286
AfaI GTAC 6 cut(s) 37, 1431, 2049, 2638, 2820, 3439
AfiI CCNNNNNNNGG 6 cut(s) 32, 500, 1659, 1660, 1679, 3264
AflII CTTAAG 1 cut(s) 2894
AflIII ACRYGT 2 cut(s) 381, 530
AhlI ACTAGT 1 cut(s) 830
AjnI CCWGG 4 cut(s) 31, 488, 1872, 2386
AleI CACNNNNGTG 1 cut(s) 1980
Alw26I GTCTC 6 cut(s) 396, 521, 1183, 2296, 2517, 3172
AoxI GGCC 7 cut(s) 359, 491, 1027, 1096, 1951, 3041, 3368
ApeKI GCWGC 9 cut(s) 597, 791, 1760, 2198, 2706, 2828, 2948, 3156, 3159
ApoI RAATTY 9 cut(s) 130, 455, 844, 936, 1489, 1562, 2116, 2266, 3198
Asp700I GAANNNNTTC 2 cut(s) 675, 3430
AspS9I GGNCC 3 cut(s) 13, 1027, 2027
AsuC2I CCSGG 1 cut(s) 3164
AvaII GGWCC 2 cut(s) 13, 2027
AxyI CCTNAGG 1 cut(s) 2879
BaeI ACNNNNGTAYC 2 cut(s) 3081, 3114
BalI TGGCCA 1 cut(s) 1098
BamHI GGATCC 2 cut(s) 2873, 3031
BanI GGYRCC 2 cut(s) 3133, 3254
BauI CACGAG 1 cut(s) 1849
BbsI GAAGAC 3 cut(s) 960, 1480, 1832
BbvI GCAGC 9 cut(s) 609, 778, 1772, 2185, 2693, 2840, 2960, 3168, 3171
BccI CCATC 8 cut(s) 293, 494, 1112, 1888, 1979, 2134, 3124, 3266
BceAI ACGGC 2 cut(s) 3056, 3355
BcgI CGANNNNNNTGC 2 cut(s) 1684, 1718
BciT130I CCWGG 4 cut(s) 33, 490, 1874, 2388
BciVI GTATCC 3 cut(s) 2227, 2338, 3109
BclI TGATCA 1 cut(s) 2164
BcnI CCSGG 1 cut(s) 3164
BcoDI GTCTC 6 cut(s) 396, 521, 1183, 2296, 2517, 3172
BcuI ACTAGT 1 cut(s) 830
BfaI CTAG 5 cut(s) 495, 822, 831, 1968, 3017
BfmI CTRYAG 4 cut(s) 135, 1466, 1758, 2850
BfrI CTTAAG 1 cut(s) 2894
BfuI GTATCC 3 cut(s) 2227, 2338, 3109
BglII AGATCT 3 cut(s) 673, 1192, 3213
BlpI GCTNAGC 1 cut(s) 272
Bme1390I CCNGG 5 cut(s) 33, 490, 1874, 2388, 3164
Bme18I GGWCC 2 cut(s) 13, 2027
BmgT120I GGNCC 3 cut(s) 13, 1027, 2027
BmiI GGNNCC 9 cut(s) 696, 1028, 1216, 1755, 2875, 3033, 3135, 3231, 3256
BmrFI CCNGG 5 cut(s) 33, 490, 1874, 2388, 3164
BmrI ACTGGG 2 cut(s) 972, 1689
BmsI GCATC 9 cut(s) 206, 634, 934, 1125, 1648, 2487, 2536, 2815, 2935
BmuI ACTGGG 2 cut(s) 972, 1689
BpiI GAAGAC 3 cut(s) 960, 1480, 1832
BplI GAGNNNNNCTC 2 cut(s) 3019, 3051
BpmI CTGGAG 2 cut(s) 15, 3243
Bpu1102I GCTNAGC 1 cut(s) 272
BpuEI CTTGAG 3 cut(s) 1284, 2168, 3046
BpuMI CCSGG 1 cut(s) 3164
BsaAI YACGTR 1 cut(s) 3441
BsaBI GATNNNNATC 1 cut(s) 672
BsaHI GRCGYC 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 3172
BsaJI CCNNGG 4 cut(s) 488, 698, 1424, 3125
BsaWI WCCGGW 2 cut(s) 587, 3101
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 500, 1659, 1660, 1679, 3264
Bse118I RCCGGY 1 cut(s) 2916
Bse1I ACTGG 8 cut(s) 967, 1139, 1482, 1684, 1764, 1836, 2256, 3226
Bse21I CCTNAGG 1 cut(s) 2879
Bse3DI GCAATG 2 cut(s) 1028, 2556
Bse8I GATNNNNATC 1 cut(s) 672
BseBI CCWGG 4 cut(s) 33, 490, 1874, 2388
BseDI CCNNGG 4 cut(s) 488, 698, 1424, 3125
BseGI GGATG 7 cut(s) 1899, 2050, 2509, 2683, 2788, 2950, 3135
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 6 cut(s) 32, 500, 1659, 1660, 1679, 3264
BseMI GCAATG 2 cut(s) 1028, 2556
BseNI ACTGG 8 cut(s) 967, 1139, 1482, 1684, 1764, 1836, 2256, 3226
BseRI GAGGAG 3 cut(s) 190, 384, 707
BseX3I CGGCCG 1 cut(s) 3368
BseXI GCAGC 9 cut(s) 609, 778, 1772, 2185, 2693, 2840, 2960, 3168, 3171
Bsh1236I CGCG 1 cut(s) 532
Bsh1285I CGRYCG 1 cut(s) 3371
BshFI GGCC 7 cut(s) 361, 493, 1029, 1098, 1953, 3043, 3370
BshNI GGYRCC 2 cut(s) 3133, 3254
BsiEI CGRYCG 1 cut(s) 3371
BsiSI CCGG 4 cut(s) 588, 2917, 3102, 3163
BslFI GGGAC 2 cut(s) 2349, 2398
BslI CCNNNNNNNGG 6 cut(s) 32, 500, 1659, 1660, 1679, 3264
BsmAI GTCTC 6 cut(s) 396, 521, 1183, 2296, 2517, 3172
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 2 cut(s) 2349, 2398
BsmI GAATGC 2 cut(s) 2391, 2971
BsnI GGCC 7 cut(s) 361, 493, 1029, 1098, 1953, 3043, 3370
Bso31I GGTCTC 1 cut(s) 3172
Bsp1407I TGTACA 1 cut(s) 2047
Bsp1720I GCTNAGC 1 cut(s) 272
Bsp19I CCATGG 1 cut(s) 3125
BspACI CCGC 4 cut(s) 221, 1928, 2360, 2668
BspANI GGCC 7 cut(s) 361, 493, 1029, 1098, 1953, 3043, 3370
BspFNI CGCG 1 cut(s) 532
BspHI TCATGA 2 cut(s) 1555, 1642
BspLI GGNNCC 9 cut(s) 696, 1028, 1216, 1755, 2875, 3033, 3135, 3231, 3256
BspMAI CTGCAG 2 cut(s) 1470, 1762
BspQI GCTCTTC 1 cut(s) 561
BspT107I GGYRCC 2 cut(s) 3133, 3254
BspTI CTTAAG 1 cut(s) 2894
BspTNI GGTCTC 1 cut(s) 3172
BsrDI GCAATG 2 cut(s) 1028, 2556
BsrFI RCCGGY 1 cut(s) 2916
BsrGI TGTACA 1 cut(s) 2047
BsrI ACTGG 8 cut(s) 967, 1139, 1482, 1684, 1764, 1836, 2256, 3226
BssAI RCCGGY 1 cut(s) 2916
BssECI CCNNGG 4 cut(s) 488, 698, 1424, 3125
BssNI GRCGYC 1 cut(s) 70
BssSI CACGAG 1 cut(s) 1849
BssT1I CCWWGG 2 cut(s) 1424, 3125
Bst2BI CACGAG 1 cut(s) 1849
Bst2UI CCWGG 4 cut(s) 33, 490, 1874, 2388
Bst6I CTCTTC 4 cut(s) 561, 1691, 3291, 3333
BstACI GRCGYC 1 cut(s) 70
BstAFI CTTAAG 1 cut(s) 2894
BstAUI TGTACA 1 cut(s) 2047
BstBAI YACGTR 1 cut(s) 3441
BstC8I GCNNGC 5 cut(s) 195, 602, 1238, 2886, 3402
BstDSI CCRYGG 2 cut(s) 698, 3125
BstEII GGTNACC 1 cut(s) 3348
BstF5I GGATG 7 cut(s) 1899, 2050, 2509, 2683, 2788, 2950, 3135
BstFNI CGCG 1 cut(s) 532
BstMAI GTCTC 6 cut(s) 396, 521, 1183, 2296, 2517, 3172
BstMCI CGRYCG 1 cut(s) 3371
BstMWI GCNNNNNNNGC 6 cut(s) 107, 1925, 2280, 2852, 2926, 2945
BstNI CCWGG 4 cut(s) 33, 490, 1874, 2388
BstNSI RCATGY 3 cut(s) 1240, 1247, 2888
BstPI GGTNACC 1 cut(s) 3348
BstSCI CCNGG 5 cut(s) 31, 488, 1872, 2386, 3162
BstSFI CTRYAG 4 cut(s) 135, 1466, 1758, 2850
BstSNI TACGTA 1 cut(s) 3441
BstUI CGCG 1 cut(s) 532
BstV1I GCAGC 9 cut(s) 609, 778, 1772, 2185, 2693, 2840, 2960, 3168, 3171
BstV2I GAAGAC 3 cut(s) 960, 1480, 1832
BstXI CCANNNNNNTGG 2 cut(s) 1037, 1106
BstZI CGGCCG 1 cut(s) 3368
Bsu36I CCTNAGG 1 cut(s) 2879
BsuI GTATCC 3 cut(s) 2227, 2338, 3109
BsuRI GGCC 7 cut(s) 361, 493, 1029, 1098, 1953, 3043, 3370
BtgI CCRYGG 2 cut(s) 698, 3125
BtgZI GCGATG 1 cut(s) 3322
BtsCI GGATG 7 cut(s) 1899, 2050, 2509, 2683, 2788, 2950, 3135
BtsIMutI CAGTG 8 cut(s) 144, 1146, 1414, 1677, 1771, 1800, 2263, 2380
Cac8I GCNNGC 5 cut(s) 195, 602, 1238, 2886, 3402
CciI TCATGA 2 cut(s) 1555, 1642
Cfr10I RCCGGY 1 cut(s) 2916
Cfr13I GGNCC 3 cut(s) 13, 1027, 2027
CseI GACGC 3 cut(s) 78, 538, 3337
Csp6I GTAC 6 cut(s) 36, 1430, 2048, 2637, 2819, 3438
CspCI CAANNNNNGTGG 2 cut(s) 2451, 2486
CviQI GTAC 6 cut(s) 36, 1430, 2048, 2637, 2819, 3438
DraI TTTAAA 2 cut(s) 916, 2206
DraIII CACNNNGTG 1 cut(s) 286
DrdI GACNNNNNNGTC 1 cut(s) 1175
DseDI GACNNNNNNGTC 1 cut(s) 1175
EaeI YGGCCR 3 cut(s) 359, 1096, 3368
EagI CGGCCG 1 cut(s) 3368
Eam1104I CTCTTC 4 cut(s) 561, 1691, 3291, 3333
EarI CTCTTC 4 cut(s) 561, 1691, 3291, 3333
EclXI CGGCCG 1 cut(s) 3368
Eco105I TACGTA 1 cut(s) 3441
Eco130I CCWWGG 2 cut(s) 1424, 3125
Eco147I AGGCCT 1 cut(s) 493
Eco31I GGTCTC 1 cut(s) 3172
Eco47I GGWCC 2 cut(s) 13, 2027
Eco52I CGGCCG 1 cut(s) 3368
Eco57I CTGAAG 3 cut(s) 1071, 1962, 3404
Eco81I CCTNAGG 1 cut(s) 2879
Eco91I GGTNACC 1 cut(s) 3348
EcoO65I GGTNACC 1 cut(s) 3348
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 4 cut(s) 31, 488, 1872, 2386
EcoT14I CCWWGG 2 cut(s) 1424, 3125
EcoT22I ATGCAT 1 cut(s) 3249
ErhI CCWWGG 2 cut(s) 1424, 3125
Esp3I CGTCTC 1 cut(s) 521
FalI AAGNNNNNCTT 2 cut(s) 2693, 2725
FaqI GGGAC 2 cut(s) 2349, 2398
FauI CCCGC 1 cut(s) 2661
FauNDI CATATG 5 cut(s) 76, 609, 2169, 2419, 2641
FbaI TGATCA 1 cut(s) 2164
FblI GTMKAC 1 cut(s) 1082
FokI GGATG 7 cut(s) 1906, 2037, 2516, 2690, 2775, 2957, 3142
FspBI CTAG 5 cut(s) 495, 822, 831, 1968, 3017
GsuI CTGGAG 2 cut(s) 15, 3243
HaeIII GGCC 7 cut(s) 361, 493, 1029, 1098, 1953, 3043, 3370
HapII CCGG 4 cut(s) 588, 2917, 3102, 3163
HgaI GACGC 3 cut(s) 78, 538, 3337
Hin1I GRCGYC 1 cut(s) 70
HincII GTYRAC 5 cut(s) 751, 866, 2072, 2428, 3022
HindII GTYRAC 5 cut(s) 751, 866, 2072, 2428, 3022
HindIII AAGCTT 1 cut(s) 2927
HpaII CCGG 4 cut(s) 588, 2917, 3102, 3163
Hpy166II GTNNAC 8 cut(s) 379, 751, 866, 1083, 2072, 2371, 2428, 3022
Hpy8I GTNNAC 8 cut(s) 379, 751, 866, 1083, 2072, 2371, 2428, 3022
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 5 cut(s) 283, 302, 313, 1298, 3169
HpyCH4IV ACGT 5 cut(s) 381, 1104, 1551, 1990, 3440
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 1925, 2280, 2852, 2926, 2945
HpySE526I ACGT 5 cut(s) 381, 1104, 1551, 1990, 3440
Hsp92I GRCGYC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 2164
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 373, 694
Lsp1109I GCAGC 9 cut(s) 609, 778, 1772, 2185, 2693, 2840, 2960, 3168, 3171
LweI GCATC 9 cut(s) 206, 634, 934, 1125, 1648, 2487, 2536, 2815, 2935
MaeI CTAG 5 cut(s) 495, 822, 831, 1968, 3017
MaeII ACGT 5 cut(s) 381, 1104, 1551, 1990, 3440
MfeI CAATTG 2 cut(s) 1346, 2738
MlsI TGGCCA 1 cut(s) 1098
MluI ACGCGT 1 cut(s) 530
MluNI TGGCCA 1 cut(s) 1098
MlyI GAGTC 5 cut(s) 194, 398, 734, 1463, 2498
MmeI TCCRAC 6 cut(s) 307, 1191, 1665, 1840, 2322, 3037
Mox20I TGGCCA 1 cut(s) 1098
Mph1103I ATGCAT 1 cut(s) 3249
MroXI GAANNNNTTC 2 cut(s) 675, 3430
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 6 cut(s) 411, 915, 1328, 2205, 2697, 2895
MslI CAYNNNNRTG 5 cut(s) 1644, 1980, 2793, 3124, 3252
Msp20I TGGCCA 1 cut(s) 1098
MspA1I CMGCKG 2 cut(s) 1091, 1928
MspCI CTTAAG 1 cut(s) 2894
MspI CCGG 4 cut(s) 588, 2917, 3102, 3163
MspR9I CCNGG 5 cut(s) 33, 490, 1874, 2388, 3164
MunI CAATTG 2 cut(s) 1346, 2738
Mva1269I GAATGC 2 cut(s) 2391, 2971
MvaI CCWGG 4 cut(s) 33, 490, 1874, 2388
MvnI CGCG 1 cut(s) 532
MwoI GCNNNNNNNGC 6 cut(s) 107, 1925, 2280, 2852, 2926, 2945
NciI CCSGG 1 cut(s) 3164
NcoI CCATGG 1 cut(s) 3125
NdeI CATATG 5 cut(s) 76, 609, 2169, 2419, 2641
NlaIV GGNNCC 9 cut(s) 696, 1028, 1216, 1755, 2875, 3033, 3135, 3231, 3256
NmuCI GTSAC 8 cut(s) 1049, 1109, 1409, 1547, 1675, 1846, 3005, 3348
NsiI ATGCAT 1 cut(s) 3249
NspI RCATGY 3 cut(s) 1240, 1247, 2888
OliI CACNNNNGTG 1 cut(s) 1980
PaeI GCATGC 2 cut(s) 1240, 2888
PagI TCATGA 2 cut(s) 1555, 1642
PceI AGGCCT 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 561
PctI GAATGC 2 cut(s) 2391, 2971
PdmI GAANNNNTTC 2 cut(s) 675, 3430
PflFI GACNNNGTC 1 cut(s) 964
PleI GAGTC 5 cut(s) 193, 397, 733, 1462, 2497
PpsI GAGTC 5 cut(s) 193, 397, 733, 1462, 2497
Ppu21I YACGTR 1 cut(s) 3441
PsiI TTATAA 2 cut(s) 123, 2933
Psp1406I AACGTT 1 cut(s) 1104
Psp6I CCWGG 4 cut(s) 31, 488, 1872, 2386
PspEI GGTNACC 1 cut(s) 3348
PspGI CCWGG 4 cut(s) 31, 488, 1872, 2386
PspN4I GGNNCC 9 cut(s) 696, 1028, 1216, 1755, 2875, 3033, 3135, 3231, 3256
PspPI GGNCC 3 cut(s) 13, 1027, 2027
PstI CTGCAG 2 cut(s) 1470, 1762
PsyI GACNNNGTC 1 cut(s) 964
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 6 cut(s) 37, 1431, 2049, 2638, 2820, 3439
RsaNI GTAC 6 cut(s) 36, 1430, 2048, 2637, 2819, 3438
RseI CAYNNNNRTG 5 cut(s) 1644, 1980, 2793, 3124, 3252
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 6 cut(s) 411, 915, 1328, 2205, 2697, 2895
Sau96I GGNCC 3 cut(s) 13, 1027, 2027
SchI GAGTC 5 cut(s) 194, 398, 734, 1463, 2498
ScrFI CCNGG 5 cut(s) 33, 490, 1874, 2388, 3164
SfaNI GCATC 9 cut(s) 206, 634, 934, 1125, 1648, 2487, 2536, 2815, 2935
SfcI CTRYAG 4 cut(s) 135, 1466, 1758, 2850
SinI GGWCC 2 cut(s) 13, 2027
SmiMI CAYNNNNRTG 5 cut(s) 1644, 1980, 2793, 3124, 3252
SmlI CTYRAG 4 cut(s) 1299, 2147, 2894, 3025
SmoI CTYRAG 4 cut(s) 1299, 2147, 2894, 3025
SnaBI TACGTA 1 cut(s) 3441
SpeI ACTAGT 1 cut(s) 830
SphI GCATGC 2 cut(s) 1240, 2888
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 4 cut(s) 221, 1928, 2360, 2668
SspI AATATT 1 cut(s) 2983
SspMI CTAG 5 cut(s) 495, 822, 831, 1968, 3017
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 5 cut(s) 31, 488, 1872, 2386, 3162
StyI CCWWGG 2 cut(s) 1424, 3125
TaiI ACGT 5 cut(s) 384, 1107, 1554, 1993, 3443
TaqI TCGA 5 cut(s) 67, 346, 764, 2129, 3289
TaqII GACCGA 1 cut(s) 405
TatI WGTACW 1 cut(s) 2047
TauI GCSGC 2 cut(s) 224, 1931
Tru1I TTAA 6 cut(s) 411, 915, 1328, 2205, 2697, 2895
Tru9I TTAA 6 cut(s) 411, 915, 1328, 2205, 2697, 2895
TscAI CASTG 8 cut(s) 144, 1146, 1414, 1684, 1771, 1800, 2263, 2380
TseFI GTSAC 8 cut(s) 1049, 1109, 1409, 1547, 1675, 1846, 3005, 3348
TseI GCWGC 9 cut(s) 597, 791, 1760, 2198, 2706, 2828, 2948, 3156, 3159
Tsp45I GTSAC 8 cut(s) 1049, 1109, 1409, 1547, 1675, 1846, 3005, 3348
TspGWI ACGGA 1 cut(s) 2673
TspRI CASTG 8 cut(s) 144, 1146, 1414, 1684, 1771, 1800, 2263, 2380
Tth111I GACNNNGTC 1 cut(s) 964
Vha464I CTTAAG 1 cut(s) 2894
VpaK11BI GGWCC 2 cut(s) 13, 2027
XapI RAATTY 9 cut(s) 130, 455, 844, 936, 1489, 1562, 2116, 2266, 3198
XceI RCATGY 3 cut(s) 1240, 1247, 2888
XmiI GTMKAC 1 cut(s) 1082
XmnI GAANNNNTTC 2 cut(s) 675, 3430
XspI CTAG 5 cut(s) 495, 822, 831, 1968, 3017
Zsp2I ATGCAT 1 cut(s) 3249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.