AT3G25410

sodium metabolite cotransporter BASS3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008339
Physical Location & Seq
Reverse (-)
9214278 .. 9216663
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25410.1

Sequence Viewer

Length: 1296 bp
ATGACTTTAATCGCTTCTCTGTCTCTCCCACCACCAGCTATACAAAGACAATCACTTTCTGAATTTCATAATCTTCCCGCTAAATCTCAATCCTTCAATTGTCAATTCCGGTCTTCTAGTTCACCTTTATCTTCTCTCCATTCTTCATCGCTTAGTAACTTTCTAGATTTTAGACTAAGGCGGAGAAACAGCGGATTGGTTCCAGTGGTGGCGTGTTCAACGACGCCGTTTATGGGCAGAGTTGGATTACATTGGAGAGATGGGAATATGTCTCTGTTATCGTTTTGTGGTGGAACTGATGTTACAGAGAAAGCTGATTCGTCTCAGTTTTGGTCTGCTTTGCTTCCATTTGTTGTTGCTCTCACTGCCGTTGCTGCTCTCTCTTACCCACCTAGTTTCACTTGGGTATCTAAAGACCTCTATGCTCCTGCTCTTGGAGGGATTATGTTATCTATTGGAATTCAGCTTTCAGTTGACGATTTTGCTCTTGCATTCAAAAGACCAGTGCCGTTATCTGTTGGGTTTGTTGCTCAGTACGTTTTGAAACCGCTTCTCGGGGTTTTAGTTGCTAATGCATTTGGGATGCCGAGGACCTTCTACGCTGGTTTTATACTCACGTGTTGTGTGGCTGGGGCTCAGTTGTCTAGTTACGCGAGTTCTCTGAGTAAAGCTGATGTGGCCATGAGCATTCTTCTCACTAGTTCTACAACTATTGCTTCTGTAATCTTTACACCTTTGCTTAGTGGTCTTCTAATTGGATCAGTAGTTCCTGTTGATGCAGTTGCCATGTCGAAGTCTATACTTCAGGTGGTACTTGTTCCAATCACTCTTGGCCTTGTGTTAAATACTTATGCGAAGCCTGTGGTCACTCTTCTTCAACCTGTGATGCCGTTTGTTGCTATGGTATGCACTTCGTTGTGCATTGGAAGCCCACTTTCGATTAATCGCAGTCAGATTCTGTCAGCTGAAGGTCTTGGATTGATTGTTCCAATTGTTACATTCCATGCCGTAGCATTTGCCTTAGGATATTGGTTCTCTAAGATCCCAGGCTTACGACAAGAGGAAGAAGTAAGCCGGACTATCTCCCTTTGCACGGGAATGCAAAGCTCCACATTAGCCGGGCTTCTTGCAAGCCAGTTCCTTGGTAGCAGCCAAGCCGTGCCAGCAGCTTGCTCTGTAGTTGTAATGGCCATAATGGGTCTATGCCTTGCCTCTTTCTGGGGCAATGGGTTTCGAATCAGGGACGTTCTATCGCTATCAACACCGCAAAGCACTGGCTACACCGCTGAATCATGA

Protein Analysis

431

Amino Acids

45.56

Weight (kDa)

8.94

Isoelectric Point (pI)

56.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 143 - 321 2.5e-33 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 653
AciI CCGC 6 cut(s) 78, 181, 192, 548, 1265, 1284
AclWI GGATC 2 cut(s) 766, 1036
AcoI YGGCCR 2 cut(s) 678, 1188
AcsI RAATTY 2 cut(s) 62, 459
AcuI CTGAAG 2 cut(s) 788, 987
AcvI CACGTG 1 cut(s) 618
AcyI GRCGYC 1 cut(s) 224
AfaI GTAC 2 cut(s) 536, 813
AfiI CCNNNNNNNGG 5 cut(s) 233, 434, 554, 1093, 1218
AflIII ACRYGT 1 cut(s) 617
AgsI TTSAA 5 cut(s) 97, 219, 496, 544, 878
AhlI ACTAGT 1 cut(s) 698
AjnI CCWGG 1 cut(s) 1045
AloI GAACNNNNNNTCC 4 cut(s) 750, 782, 969, 1001
AluBI AGCT 7 cut(s) 38, 314, 466, 671, 965, 1107, 1169
AluI AGCT 7 cut(s) 38, 314, 466, 671, 965, 1107, 1169
Alw26I GTCTC 3 cut(s) 27, 276, 327
AlwI GGATC 2 cut(s) 766, 1036
AlwNI CAGNNNCTG 1 cut(s) 958
Ama87I CYCGRG 1 cut(s) 554
AoxI GGCC 3 cut(s) 678, 832, 1188
ApeKI GCWGC 3 cut(s) 374, 1149, 1166
ApoI RAATTY 2 cut(s) 62, 459
AseI ATTAAT 1 cut(s) 942
AspS9I GGNCC 1 cut(s) 591
AsuC2I CCSGG 1 cut(s) 1120
AsuHPI GGTGA 1 cut(s) 114
AsuII TTCGAA 1 cut(s) 1234
AvaI CYCGRG 1 cut(s) 554
AvaII GGWCC 1 cut(s) 591
AxyI CCTNAGG 1 cut(s) 1021
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BalI TGGCCA 2 cut(s) 680, 1190
BanII GRGCYC 1 cut(s) 637
BbrPI CACGTG 1 cut(s) 618
BbsI GAAGAC 2 cut(s) 105, 740
BbvI GCAGC 3 cut(s) 361, 1161, 1178
BccI CCATC 1 cut(s) 254
BceAI ACGGC 6 cut(s) 211, 353, 493, 874, 992, 1142
BciT130I CCWGG 1 cut(s) 1047
BcnI CCSGG 1 cut(s) 1120
BcoDI GTCTC 3 cut(s) 27, 276, 327
BcuI ACTAGT 1 cut(s) 698
BfaI CTAG 5 cut(s) 117, 164, 393, 645, 699
BfmI CTRYAG 1 cut(s) 1176
BisI GCNGC 3 cut(s) 375, 1150, 1167
BlsI GCNGC 3 cut(s) 376, 1151, 1168
Bme1390I CCNGG 2 cut(s) 1047, 1120
Bme18I GGWCC 1 cut(s) 591
BmeT110I CYCGRG 1 cut(s) 554
BmgT120I GGNCC 1 cut(s) 591
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 1047, 1120
BmsI GCATC 3 cut(s) 573, 766, 876
BpiI GAAGAC 2 cut(s) 105, 740
Bpu14I TTCGAA 1 cut(s) 1234
BpuMI CCSGG 1 cut(s) 1120
BsaAI YACGTR 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 587, 1045, 1141
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 5 cut(s) 233, 434, 554, 1093, 1218
Bse1I ACTGG 4 cut(s) 203, 503, 1135, 1279
Bse21I CCTNAGG 1 cut(s) 1021
Bse3DI GCAATG 1 cut(s) 1231
BseBI CCWGG 1 cut(s) 1047
BseDI CCNNGG 3 cut(s) 587, 1045, 1141
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 5 cut(s) 233, 434, 554, 1093, 1218
BseMI GCAATG 1 cut(s) 1231
BseMII CTCAG 4 cut(s) 338, 545, 650, 653
BseNI ACTGG 4 cut(s) 203, 503, 1135, 1279
BseXI GCAGC 3 cut(s) 361, 1161, 1178
BseYI CCCAGC 1 cut(s) 629
Bsh1236I CGCG 1 cut(s) 653
BshFI GGCC 3 cut(s) 680, 834, 1190
BsiHKCI CYCGRG 1 cut(s) 554
BsiSI CCGG 3 cut(s) 109, 1075, 1119
BslFI GGGAC 1 cut(s) 1256
BslI CCNNNNNNNGG 5 cut(s) 233, 434, 554, 1093, 1218
BsmAI GTCTC 3 cut(s) 27, 276, 327
BsmBI CGTCTC 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 1256
BsmI GAATGC 3 cut(s) 491, 687, 1104
BsnI GGCC 3 cut(s) 680, 834, 1190
BsoBI CYCGRG 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 1234
Bsp1286I GDGCHC 1 cut(s) 637
Bsp143I GATC 2 cut(s) 758, 1041
BspACI CCGC 6 cut(s) 78, 181, 192, 548, 1265, 1284
BspANI GGCC 3 cut(s) 680, 834, 1190
BspCNI CTCAG 4 cut(s) 337, 544, 649, 654
BspFNI CGCG 1 cut(s) 653
BspHI TCATGA 1 cut(s) 1292
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 2 cut(s) 766, 1036
BspT104I TTCGAA 1 cut(s) 1234
BsrDI GCAATG 1 cut(s) 1231
BsrI ACTGG 4 cut(s) 203, 503, 1135, 1279
BssECI CCNNGG 3 cut(s) 587, 1045, 1141
BssMI GATC 2 cut(s) 758, 1041
BssNI GRCGYC 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 1141
Bst2UI CCWGG 1 cut(s) 1047
Bst6I CTCTTC 1 cut(s) 876
BstACI GRCGYC 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 618
BstBI TTCGAA 1 cut(s) 1234
BstC8I GCNNGC 3 cut(s) 1132, 1164, 1171
BstDEI CTNAG 9 cut(s) 152, 176, 324, 531, 636, 662, 740, 1021, 1038
BstF5I GGATG 1 cut(s) 588
BstFNI CGCG 1 cut(s) 653
BstKTI GATC 2 cut(s) 761, 1044
BstMAI GTCTC 3 cut(s) 27, 276, 327
BstMBI GATC 2 cut(s) 758, 1041
BstMWI GCNNNNNNNGC 5 cut(s) 365, 374, 677, 927, 1163
BstNI CCWGG 1 cut(s) 1047
BstSCI CCNGG 2 cut(s) 1045, 1118
BstSFI CTRYAG 1 cut(s) 1176
BstUI CGCG 1 cut(s) 653
BstV1I GCAGC 3 cut(s) 361, 1161, 1178
BstV2I GAAGAC 2 cut(s) 105, 740
BstX2I RGATCY 1 cut(s) 1041
BstXI CCANNNNNNTGG 1 cut(s) 1142
BstYI RGATCY 1 cut(s) 1041
Bsu36I CCTNAGG 1 cut(s) 1021
BsuRI GGCC 3 cut(s) 680, 834, 1190
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 1 cut(s) 588
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 4 cut(s) 210, 363, 510, 1272
Cac8I GCNNGC 3 cut(s) 1132, 1164, 1171
CaiI CAGNNNCTG 1 cut(s) 958
CciI TCATGA 1 cut(s) 1292
Cfr13I GGNCC 1 cut(s) 591
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 535, 812
CviAII CATG 4 cut(s) 682, 787, 1004, 1293
CviQI GTAC 2 cut(s) 535, 812
DdeI CTNAG 9 cut(s) 152, 176, 324, 531, 636, 662, 740, 1021, 1038
DpnI GATC 2 cut(s) 760, 1043
DpnII GATC 2 cut(s) 758, 1041
EaeI YGGCCR 2 cut(s) 678, 1188
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
EciI GGCGGA 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 1141
Eco24I GRGCYC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 591
Eco57I CTGAAG 2 cut(s) 788, 987
Eco72I CACGTG 1 cut(s) 618
Eco81I CCTNAGG 1 cut(s) 1021
Eco88I CYCGRG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 591
EcoRI GAATTC 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 1045
EcoT14I CCWWGG 1 cut(s) 1141
EcoT22I ATGCAT 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 637
ErhI CCWWGG 1 cut(s) 1141
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 4 cut(s) 685, 790, 1007, 1296
FaqI GGGAC 1 cut(s) 1256
FatI CATG 4 cut(s) 681, 786, 1003, 1292
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 3 cut(s) 375, 1150, 1167
FokI GGATG 1 cut(s) 595
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 3 cut(s) 375, 1150, 1167
FspBI CTAG 5 cut(s) 117, 164, 393, 645, 699
GluI GCNGC 3 cut(s) 375, 1150, 1167
GsaI CCCAGC 1 cut(s) 633
HaeIII GGCC 3 cut(s) 680, 834, 1190
HapII CCGG 3 cut(s) 109, 1075, 1119
HgaI GACGC 1 cut(s) 232
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 4 cut(s) 685, 790, 1007, 1296
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 4 cut(s) 317, 955, 1236, 1289
HpaII CCGG 3 cut(s) 109, 1075, 1119
HphI GGTGA 1 cut(s) 114
Hpy166II GTNNAC 2 cut(s) 122, 475
Hpy188I TCNGA 3 cut(s) 61, 663, 954
Hpy188III TCNNGA 2 cut(s) 164, 1293
Hpy8I GTNNAC 2 cut(s) 122, 475
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 3 cut(s) 103, 604, 962
HpyCH4IV ACGT 3 cut(s) 537, 617, 1245
HpyCH4V TGCA 8 cut(s) 491, 575, 779, 909, 921, 1092, 1102, 1130
HpyF10VI GCNNNNNNNGC 5 cut(s) 365, 374, 677, 927, 1163
HpyF3I CTNAG 9 cut(s) 152, 176, 324, 531, 636, 662, 740, 1021, 1038
HpySE526I ACGT 3 cut(s) 537, 617, 1245
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 4 cut(s) 685, 790, 1007, 1296
Kzo9I GATC 2 cut(s) 758, 1041
LmnI GCTCC 2 cut(s) 430, 1112
Lsp1109I GCAGC 3 cut(s) 361, 1161, 1178
LweI GCATC 3 cut(s) 573, 766, 876
MaeI CTAG 5 cut(s) 117, 164, 393, 645, 699
MaeII ACGT 3 cut(s) 537, 617, 1245
MaeIII GTNAC 5 cut(s) 155, 301, 647, 865, 994
MalI GATC 2 cut(s) 760, 1043
MboI GATC 2 cut(s) 758, 1041
MboII GAAGA 9 cut(s) 65, 105, 123, 135, 683, 740, 863, 866, 1076
MfeI CAATTG 2 cut(s) 97, 990
MflI RGATCY 1 cut(s) 1041
MhlI GDGCHC 1 cut(s) 637
MlsI TGGCCA 2 cut(s) 680, 1190
MluCI AATT 6 cut(s) 62, 97, 104, 459, 753, 990
MluNI TGGCCA 2 cut(s) 680, 1190
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 5 cut(s) 428, 431, 582, 1054, 1222
Mox20I TGGCCA 2 cut(s) 680, 1190
Mph1103I ATGCAT 1 cut(s) 577
MscI TGGCCA 2 cut(s) 680, 1190
MseI TTAA 3 cut(s) 8, 842, 942
MslI CAYNNNNRTG 1 cut(s) 1097
Msp20I TGGCCA 2 cut(s) 680, 1190
MspA1I CMGCKG 3 cut(s) 192, 965, 1286
MspI CCGG 3 cut(s) 109, 1075, 1119
MspR9I CCNGG 2 cut(s) 1047, 1120
MunI CAATTG 2 cut(s) 97, 990
Mva1269I GAATGC 3 cut(s) 491, 687, 1104
MvaI CCWGG 1 cut(s) 1047
MvnI CGCG 1 cut(s) 653
MwoI GCNNNNNNNGC 5 cut(s) 365, 374, 677, 927, 1163
NciI CCSGG 1 cut(s) 1120
NdeII GATC 2 cut(s) 758, 1041
NlaIII CATG 4 cut(s) 685, 790, 1007, 1296
NlaIV GGNNCC 1 cut(s) 201
NmeAIII GCCGAG 1 cut(s) 612
NmuCI GTSAC 1 cut(s) 865
NsiI ATGCAT 1 cut(s) 577
NspV TTCGAA 1 cut(s) 1234
PagI TCATGA 1 cut(s) 1292
PctI GAATGC 3 cut(s) 491, 687, 1104
PfeI GAWTC 4 cut(s) 317, 955, 1236, 1289
PkrI GCNGC 3 cut(s) 376, 1151, 1168
PmaCI CACGTG 1 cut(s) 618
PmlI CACGTG 1 cut(s) 618
Ppu21I YACGTR 1 cut(s) 618
PpuMI RGGWCCY 1 cut(s) 591
PshBI ATTAAT 1 cut(s) 942
Psp5II RGGWCCY 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 1045
PspCI CACGTG 1 cut(s) 618
PspFI CCCAGC 1 cut(s) 629
PspGI CCWGG 1 cut(s) 1045
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 591
PspPPI RGGWCCY 1 cut(s) 591
PsrI GAACNNNNNNTAC 2 cut(s) 286, 318
PstNI CAGNNNCTG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 1041
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 2 cut(s) 536, 813
RsaNI GTAC 2 cut(s) 535, 812
RseI CAYNNNNRTG 1 cut(s) 1097
SaqAI TTAA 3 cut(s) 8, 842, 942
SatI GCNGC 3 cut(s) 375, 1150, 1167
Sau3AI GATC 2 cut(s) 758, 1041
Sau96I GGNCC 1 cut(s) 591
ScrFI CCNGG 2 cut(s) 1047, 1120
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 3 cut(s) 573, 766, 876
SfcI CTRYAG 1 cut(s) 1176
SfuI TTCGAA 1 cut(s) 1234
SinI GGWCC 1 cut(s) 591
SmiMI CAYNNNNRTG 1 cut(s) 1097
SpeI ACTAGT 1 cut(s) 698
Sse9I AATT 6 cut(s) 62, 97, 104, 459, 753, 990
SsiI CCGC 6 cut(s) 78, 181, 192, 548, 1265, 1284
SspMI CTAG 5 cut(s) 117, 164, 393, 645, 699
StyD4I CCNGG 2 cut(s) 1045, 1118
StyI CCWWGG 1 cut(s) 1141
TaiI ACGT 3 cut(s) 540, 620, 1248
TaqI TCGA 3 cut(s) 791, 938, 1234
TasI AATT 6 cut(s) 62, 97, 104, 459, 753, 990
TfiI GAWTC 4 cut(s) 317, 955, 1236, 1289
Tru1I TTAA 3 cut(s) 8, 842, 942
Tru9I TTAA 3 cut(s) 8, 842, 942
TscAI CASTG 4 cut(s) 210, 370, 510, 1279
TseFI GTSAC 1 cut(s) 865
TseI GCWGC 3 cut(s) 374, 1149, 1166
Tsp45I GTSAC 1 cut(s) 865
TspDTI ATGAA 2 cut(s) 56, 135
TspRI CASTG 4 cut(s) 210, 370, 510, 1279
VpaK11BI GGWCC 1 cut(s) 591
VspI ATTAAT 1 cut(s) 942
XapI RAATTY 2 cut(s) 62, 459
XbaI TCTAGA 1 cut(s) 163
XspI CTAG 5 cut(s) 117, 164, 393, 645, 699
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.