MD10G1151500.v1.1

sodium metabolite cotransporter BASS3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008339
Physical Location & Seq
Forward (+)
23846304 .. 23849172
2869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1151500.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGTCCACCACTCCCATCTCCTATCCAATAACCTCAAGACCCTTTCTTTCTCTCGCACCCGCCACCGCAGCCTCAAAACTCTTTGCACGCCCGAATTCCCCCACCAAAAGCTCTCTATTTTCCTCCCCTTCGTCTTCCTCCTATCATTTCCTCAATAGGGTATTCAGAAATGGGGCTGCTCTGTCGATAAGAGCTTGCTCGACGTCGCCGTTTATCGGCAGAGTCGGGTCGCAGTGGAGGGAAGGCAACGCGTCGCTGCTGTCGTTTGGGATAAACCCACCAGCTCAAATATCCAAAAGGGCTTCCGACGACTCGTCGTCGTCTCAGGTCTTATCTGCAATGCTTCCTTTCGTCGTTGCTGCTACCGCCATTGCTGCTCTGGCTCAGCCGTCAACGTTCACTTGGGTATCCAAGGATTTATATGCCCCTGCTCTTGGTGGGATCATGCTGTCAATTGGAATCAAACTGTCGATTGATGATTTTGCTCTTGCATTCAAAAGACCTTTACCCCTCTCTGTTGGGTTTATCGCGCAGTATGTACTGAAACCGGTACTTGGGGTACTAATTGCTAACGCATTTGGCGTGCCAAGGATGTTCTACGCTGGCTTTGTCCTCACTGCTTGTGTTGCAGGGGCTCAGCTGTCTAGCTACGCTAGTTTCTTGAGCAAAGGAGACGTGGCCTTGAGCATTCTCCTCACCAGCACCACCACCATTGCTTCTGTGATCGTAACGCCTTTACTAACCGGCCTTTTAATCGGTTCTGTTGTTCCGGTTGATGCTGTTGCCATGTCAAAGTCGATATTGCAGGTGGTTCTTTTTCCGGTCACAATTGGTCTTGTGCTCAATACTTATGCAAAACCAGTTGTCAATTTCCTTCGACCAGTGATGCCAGTTGTTGCTATGATATGCACTTCATTGTGCATTGGCAGTCCTCTTGCAATTAACCGGAGTCAAATTCTATCTGTAGAGGGTTTCCGGTTGATATTTCCAGTCATAGCATTTCATGCGGTGGCCTTTACACTTGGATACTGGGTATCCAAAACTCCAGGTTTGAGGCAAGAAGAAGAAGTTAGCAGGACACTTTCCTTATGCACGGGAATGCAGAGTTCCACTTTGGCAGGGTTACTTGCAAGCCAATTCCTTGGGAGCAGCCAGGCCGTTCCTCCAGCATGTTCGGTTGTTGCCATGGCCATCGTGGGCCTTTTTCTTGCGTCTTTCTGGGGCACCGGCTCTCAAATCAGAGACCTTCCGTCCCTACTAACACCTCGAACAGGTTCTCCAGTGAAGGCTTAG

Protein Analysis

431

Amino Acids

45.08

Weight (kDa)

9.95

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 143 - 321 1.7e-34 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 796
AatII GACGTC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 796
AccB1I GGYRCC 1 cut(s) 1223
AccII CGCG 2 cut(s) 251, 530
AciI CCGC 4 cut(s) 60, 66, 366, 1007
AclI AACGTT 1 cut(s) 395
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 1 cut(s) 1188
AcsI RAATTY 2 cut(s) 94, 954
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 3 cut(s) 540, 552, 561
AfiI CCNNNNNNNGG 5 cut(s) 157, 215, 434, 554, 1271
AflIII ACRYGT 1 cut(s) 249
AgeI ACCGGT 1 cut(s) 547
AgsI TTSAA 1 cut(s) 496
AhdI GACNNNNNGTC 3 cut(s) 313, 316, 1249
AjiI CACGTC 1 cut(s) 676
AjnI CCWGG 2 cut(s) 1045, 1152
AloI GAACNNNNNNTCC 2 cut(s) 1261, 1293
AluBI AGCT 5 cut(s) 111, 194, 284, 640, 648
AluI AGCT 5 cut(s) 111, 194, 284, 640, 648
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 3 cut(s) 327, 666, 1236
AlwI GGATC 1 cut(s) 449
AoxI GGCC 6 cut(s) 678, 745, 1011, 1155, 1188, 1198
ApeKI GCWGC 6 cut(s) 68, 176, 256, 359, 374, 1149
ApoI RAATTY 2 cut(s) 94, 954
AsiGI ACCGGT 1 cut(s) 547
Asp700I GAANNNNTTC 1 cut(s) 1273
AspLEI GCGC 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 1198
AsuHPI GGTGA 1 cut(s) 688
BaeGI GKGCMC 1 cut(s) 1226
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BalI TGGCCA 1 cut(s) 1190
BanI GGYRCC 1 cut(s) 1223
BanII GRGCYC 1 cut(s) 637
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 6 cut(s) 80, 163, 243, 346, 361, 1161
BccI CCATC 2 cut(s) 23, 1199
BceAI ACGGC 3 cut(s) 193, 373, 1142
BciT130I CCWGG 2 cut(s) 1047, 1154
BciVI GTATCC 3 cut(s) 418, 1019, 1045
BcoDI GTCTC 3 cut(s) 327, 666, 1236
BfaI CTAG 2 cut(s) 645, 654
BfmI CTRYAG 1 cut(s) 963
BfuAI ACCTGC 1 cut(s) 796
BfuI GTATCC 3 cut(s) 418, 1019, 1045
BisI GCNGC 6 cut(s) 69, 177, 257, 360, 375, 1150
BlpI GCTNAGC 2 cut(s) 384, 636
BlsI GCNGC 6 cut(s) 70, 178, 258, 361, 376, 1151
Bme1390I CCNGG 2 cut(s) 1047, 1154
BmeRI GACNNNNNGTC 3 cut(s) 313, 316, 1249
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 1 cut(s) 1198
BmiI GGNNCC 1 cut(s) 1225
BmrFI CCNGG 2 cut(s) 1047, 1154
BmrI ACTGGG 1 cut(s) 1039
BmsI GCATC 2 cut(s) 766, 876
BmuI ACTGGG 1 cut(s) 1039
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 3 cut(s) 1029, 1149, 1263
Bpu1102I GCTNAGC 2 cut(s) 384, 636
BpuEI CTTGAG 3 cut(s) 19, 682, 703
BsaHI GRCGYC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 1236
BsaJI CCNNGG 4 cut(s) 411, 587, 1141, 1185
BsaWI WCCGGW 5 cut(s) 547, 769, 820, 945, 975
BsaXI ACNNNNNCTCC 2 cut(s) 1261, 1291
Bsc4I CCNNNNNNNGG 5 cut(s) 157, 215, 434, 554, 1271
Bse118I RCCGGY 3 cut(s) 547, 743, 1226
Bse1I ACTGG 6 cut(s) 860, 881, 890, 989, 1034, 1280
Bse3DI GCAATG 3 cut(s) 345, 369, 711
BseBI CCWGG 2 cut(s) 1047, 1154
BseDI CCNNGG 4 cut(s) 411, 587, 1141, 1185
BseGI GGATG 1 cut(s) 597
BseLI CCNNNNNNNGG 5 cut(s) 157, 215, 434, 554, 1271
BseMI GCAATG 3 cut(s) 345, 369, 711
BseMII CTCAG 3 cut(s) 338, 398, 650
BseNI ACTGG 6 cut(s) 860, 881, 890, 989, 1034, 1280
BseRI GAGGAG 1 cut(s) 683
BseSI GKGCMC 1 cut(s) 1226
BseXI GCAGC 6 cut(s) 80, 163, 243, 346, 361, 1161
Bsh1236I CGCG 2 cut(s) 251, 530
BshFI GGCC 6 cut(s) 680, 747, 1013, 1157, 1190, 1200
BshNI GGYRCC 1 cut(s) 1223
BshTI ACCGGT 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 843
BsiSI CCGG 7 cut(s) 548, 744, 770, 821, 946, 976, 1227
BslFI GGGAC 1 cut(s) 1237
BslI CCNNNNNNNGG 5 cut(s) 157, 215, 434, 554, 1271
BsmAI GTCTC 3 cut(s) 327, 666, 1236
BsmBI CGTCTC 2 cut(s) 327, 666
BsmFI GGGAC 1 cut(s) 1237
BsmI GAATGC 3 cut(s) 491, 687, 1104
BsnI GGCC 6 cut(s) 680, 747, 1013, 1157, 1190, 1200
Bso31I GGTCTC 1 cut(s) 1236
Bsp1286I GDGCHC 3 cut(s) 637, 843, 1226
Bsp143I GATC 2 cut(s) 441, 723
Bsp1720I GCTNAGC 2 cut(s) 384, 636
Bsp19I CCATGG 1 cut(s) 1185
BspACI CCGC 4 cut(s) 60, 66, 366, 1007
BspANI GGCC 6 cut(s) 680, 747, 1013, 1157, 1190, 1200
BspCNI CTCAG 3 cut(s) 337, 397, 649
BspFNI CGCG 2 cut(s) 251, 530
BspLI GGNNCC 1 cut(s) 1225
BspMI ACCTGC 1 cut(s) 796
BspPI GGATC 1 cut(s) 449
BspT107I GGYRCC 1 cut(s) 1223
BspTNI GGTCTC 1 cut(s) 1236
BsrDI GCAATG 3 cut(s) 345, 369, 711
BsrFI RCCGGY 3 cut(s) 547, 743, 1226
BsrI ACTGG 6 cut(s) 860, 881, 890, 989, 1034, 1280
BssAI RCCGGY 3 cut(s) 547, 743, 1226
BssECI CCNNGG 4 cut(s) 411, 587, 1141, 1185
BssMI GATC 2 cut(s) 441, 723
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 4 cut(s) 411, 587, 1141, 1185
Bst2UI CCWGG 2 cut(s) 1047, 1154
Bst4CI ACNGT 1 cut(s) 468
BstACI GRCGYC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 1004
BstC8I GCNNGC 5 cut(s) 88, 196, 584, 604, 1132
BstDEI CTNAG 4 cut(s) 324, 384, 636, 1290
BstDSI CCRYGG 1 cut(s) 1185
BstENI CCTNNNNNAGG 1 cut(s) 1269
BstF5I GGATG 1 cut(s) 597
BstFNI CGCG 2 cut(s) 251, 530
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 2 cut(s) 444, 726
BstMAI GTCTC 3 cut(s) 327, 666, 1236
BstMBI GATC 2 cut(s) 441, 723
BstMWI GCNNNNNNNGC 6 cut(s) 68, 365, 374, 380, 626, 1004
BstNI CCWGG 2 cut(s) 1047, 1154
BstNSI RCATGY 1 cut(s) 1173
BstSCI CCNGG 2 cut(s) 1045, 1152
BstSFI CTRYAG 1 cut(s) 963
BstSLI GKGCMC 1 cut(s) 1226
BstUI CGCG 2 cut(s) 251, 530
BstV1I GCAGC 6 cut(s) 80, 163, 243, 346, 361, 1161
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 1142
BsuI GTATCC 3 cut(s) 418, 1019, 1045
BsuRI GGCC 6 cut(s) 680, 747, 1013, 1157, 1190, 1200
BtgI CCRYGG 1 cut(s) 1185
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 1 cut(s) 597
BtsI GCAGTG 2 cut(s) 239, 615
BtsIMutI CAGTG 4 cut(s) 239, 615, 888, 1287
BveI ACCTGC 1 cut(s) 796
Cac8I GCNNGC 5 cut(s) 88, 196, 584, 604, 1132
CfoI GCGC 1 cut(s) 532
Cfr10I RCCGGY 3 cut(s) 547, 743, 1226
Cfr13I GGNCC 1 cut(s) 1198
CseI GACGC 2 cut(s) 240, 1200
Csp6I GTAC 3 cut(s) 539, 551, 560
CspAI ACCGGT 1 cut(s) 547
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 5 cut(s) 445, 787, 1004, 1170, 1186
CviQI GTAC 3 cut(s) 539, 551, 560
DdeI CTNAG 4 cut(s) 324, 384, 636, 1290
DpnI GATC 2 cut(s) 443, 725
DpnII GATC 2 cut(s) 441, 723
DriI GACNNNNNGTC 3 cut(s) 313, 316, 1249
EaeI YGGCCR 1 cut(s) 1188
Eam1105I GACNNNNNGTC 3 cut(s) 313, 316, 1249
Eco130I CCWWGG 4 cut(s) 411, 587, 1141, 1185
Eco24I GRGCYC 1 cut(s) 637
Eco31I GGTCTC 1 cut(s) 1236
EcoNI CCTNNNNNAGG 1 cut(s) 1269
EcoRI GAATTC 1 cut(s) 94
EcoRII CCWGG 2 cut(s) 1045, 1152
EcoT14I CCWWGG 4 cut(s) 411, 587, 1141, 1185
EcoT38I GRGCYC 1 cut(s) 637
ErhI CCWWGG 4 cut(s) 411, 587, 1141, 1185
Esp3I CGTCTC 2 cut(s) 327, 666
FaeI CATG 5 cut(s) 448, 790, 1007, 1173, 1189
FaqI GGGAC 1 cut(s) 1237
FatI CATG 5 cut(s) 444, 786, 1003, 1169, 1185
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 6 cut(s) 69, 177, 257, 360, 375, 1150
FokI GGATG 1 cut(s) 604
FriOI GRGCYC 1 cut(s) 637
Fsp4HI GCNGC 6 cut(s) 69, 177, 257, 360, 375, 1150
FspBI CTAG 2 cut(s) 645, 654
GlaI GCGC 1 cut(s) 531
GluI GCNGC 6 cut(s) 69, 177, 257, 360, 375, 1150
GsuI CTGGAG 3 cut(s) 1029, 1149, 1263
HaeIII GGCC 6 cut(s) 680, 747, 1013, 1157, 1190, 1200
HapII CCGG 7 cut(s) 548, 744, 770, 821, 946, 976, 1227
HgaI GACGC 2 cut(s) 240, 1200
HhaI GCGC 1 cut(s) 532
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 5 cut(s) 448, 790, 1007, 1173, 1189
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HincII GTYRAC 1 cut(s) 393
HindII GTYRAC 1 cut(s) 393
HinfI GANTC 4 cut(s) 222, 311, 459, 949
HpaII CCGG 7 cut(s) 548, 744, 770, 821, 946, 976, 1227
HphI GGTGA 1 cut(s) 688
Hpy166II GTNNAC 3 cut(s) 6, 393, 399
Hpy188I TCNGA 3 cut(s) 167, 307, 1241
Hpy188III TCNNGA 2 cut(s) 36, 661
Hpy8I GTNNAC 3 cut(s) 6, 393, 399
Hpy99I CGWCG 7 cut(s) 205, 208, 256, 311, 319, 322, 356
HpyAV CCTTC 5 cut(s) 138, 236, 884, 1256, 1279
HpyCH4III ACNGT 1 cut(s) 468
HpyCH4IV ACGT 3 cut(s) 203, 395, 675
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 365, 374, 380, 626, 1004
HpyF3I CTNAG 4 cut(s) 324, 384, 636, 1290
HpySE526I ACGT 3 cut(s) 203, 395, 675
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 5 cut(s) 448, 790, 1007, 1173, 1189
HspAI GCGC 1 cut(s) 530
Kzo9I GATC 2 cut(s) 441, 723
LmnI GCTCC 1 cut(s) 1146
Lsp1109I GCAGC 6 cut(s) 80, 163, 243, 346, 361, 1161
LweI GCATC 2 cut(s) 766, 876
MaeI CTAG 2 cut(s) 645, 654
MaeII ACGT 3 cut(s) 203, 395, 675
MaeIII GTNAC 3 cut(s) 727, 823, 1122
MalI GATC 2 cut(s) 443, 725
MboI GATC 2 cut(s) 441, 723
MboII GAAGA 3 cut(s) 126, 1073, 1076
MfeI CAATTG 2 cut(s) 453, 828
MhlI GDGCHC 3 cut(s) 637, 843, 1226
MlsI TGGCCA 1 cut(s) 1190
MluCI AATT 8 cut(s) 94, 453, 564, 828, 868, 939, 954, 1136
MluI ACGCGT 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 1190
MlyI GAGTC 3 cut(s) 231, 305, 958
MmeI TCCRAC 1 cut(s) 330
Mox20I TGGCCA 1 cut(s) 1190
MroXI GAANNNNTTC 1 cut(s) 1273
MscI TGGCCA 1 cut(s) 1190
MseI TTAA 2 cut(s) 752, 942
MslI CAYNNNNRTG 1 cut(s) 1097
Msp20I TGGCCA 1 cut(s) 1190
MspA1I CMGCKG 1 cut(s) 640
MspI CCGG 7 cut(s) 548, 744, 770, 821, 946, 976, 1227
MspR9I CCNGG 2 cut(s) 1047, 1154
MunI CAATTG 2 cut(s) 453, 828
Mva1269I GAATGC 3 cut(s) 491, 687, 1104
MvaI CCWGG 2 cut(s) 1047, 1154
MvnI CGCG 2 cut(s) 251, 530
MwoI GCNNNNNNNGC 6 cut(s) 68, 365, 374, 380, 626, 1004
NcoI CCATGG 1 cut(s) 1185
NdeII GATC 2 cut(s) 441, 723
NlaIII CATG 5 cut(s) 448, 790, 1007, 1173, 1189
NlaIV GGNNCC 1 cut(s) 1225
NmuCI GTSAC 1 cut(s) 823
NspI RCATGY 1 cut(s) 1173
PaqCI CACCTGC 1 cut(s) 796
PcsI WCGNNNNNNNCGW 3 cut(s) 206, 260, 579
PctI GAATGC 3 cut(s) 491, 687, 1104
PdmI GAANNNNTTC 1 cut(s) 1273
PfeI GAWTC 1 cut(s) 459
PinAI ACCGGT 1 cut(s) 547
PkrI GCNGC 6 cut(s) 70, 178, 258, 361, 376, 1151
PleI GAGTC 3 cut(s) 230, 305, 957
PpsI GAGTC 3 cut(s) 230, 305, 957
Psp1406I AACGTT 1 cut(s) 395
Psp6I CCWGG 2 cut(s) 1045, 1152
PspGI CCWGG 2 cut(s) 1045, 1152
PspN4I GGNNCC 1 cut(s) 1225
PspPI GGNCC 1 cut(s) 1198
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 3 cut(s) 540, 552, 561
RsaNI GTAC 3 cut(s) 539, 551, 560
RseI CAYNNNNRTG 1 cut(s) 1097
SaqAI TTAA 2 cut(s) 752, 942
SatI GCNGC 6 cut(s) 69, 177, 257, 360, 375, 1150
Sau3AI GATC 2 cut(s) 441, 723
Sau96I GGNCC 1 cut(s) 1198
SchI GAGTC 3 cut(s) 231, 305, 958
ScrFI CCNGG 2 cut(s) 1047, 1154
SduI GDGCHC 3 cut(s) 637, 843, 1226
SfaNI GCATC 2 cut(s) 766, 876
SfcI CTRYAG 1 cut(s) 963
SmiMI CAYNNNNRTG 1 cut(s) 1097
SmlI CTYRAG 3 cut(s) 34, 661, 682
SmoI CTYRAG 3 cut(s) 34, 661, 682
Sse9I AATT 8 cut(s) 94, 453, 564, 828, 868, 939, 954, 1136
SsiI CCGC 4 cut(s) 60, 66, 366, 1007
SspMI CTAG 2 cut(s) 645, 654
StyD4I CCNGG 2 cut(s) 1045, 1152
StyI CCWWGG 4 cut(s) 411, 587, 1141, 1185
TaaI ACNGT 1 cut(s) 468
TaiI ACGT 3 cut(s) 206, 398, 678
TaqI TCGA 6 cut(s) 185, 200, 470, 797, 877, 1267
TasI AATT 8 cut(s) 94, 453, 564, 828, 868, 939, 954, 1136
TatI WGTACW 1 cut(s) 538
TfiI GAWTC 1 cut(s) 459
Tru1I TTAA 2 cut(s) 752, 942
Tru9I TTAA 2 cut(s) 752, 942
TscAI CASTG 4 cut(s) 239, 622, 888, 1287
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 6 cut(s) 68, 176, 256, 359, 374, 1149
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 2 cut(s) 903, 992
TspGWI ACGGA 1 cut(s) 1239
TspRI CASTG 4 cut(s) 239, 622, 888, 1287
XagI CCTNNNNNAGG 1 cut(s) 1269
XapI RAATTY 2 cut(s) 94, 954
XceI RCATGY 1 cut(s) 1173
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 1192
XmnI GAANNNNTTC 1 cut(s) 1273
XspI CTAG 2 cut(s) 645, 654
ZraI GACGTC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.