RLG00000012871

sodium metabolite cotransporter BASS3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008339
Physical Location & Seq
Reverse (-)
23378723 .. 23381215
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012871

Sequence Viewer

Length: 1254 bp
ATGTCCACTTCTTCCATACCCTTCATTTCTCTCGCACCCAACAGCTCAAAACTTCTGCTAAAGCCAAGTACATGCCCACCGAGACTGACACTATCAAGCTCTTCATTTTCGAAGAGGGGGTTCAGAAATGGGGGTCTGTCGATAAAGGGTTGCTCGACGTCGCCGTTTATAGGAAGAGTTGGGTCGCAGCGGAGGGAAGGAAACTCTTCGTTGCTTTCGTTTGGGATAAACCCACCAACGGCTATGTCCAAAGGAGCCGAACACAGTGGTGAGGACTCGTCTCAGGTCTTATCCGCAATGCTTCCATTTGTGGTTGCTGCCACTGCTATTGCTGCTTTGGCTCAGCCTGCCACGTTTACTTGGGTATCCAAGGATTTATACGCCCCTGCTCTTGGTGGGATCATGCTCTCCATTGGAATCAAACTTTCGGTCGATGATTTTGCTCTTGCTGTCAAAAGACCTTTACGACTCTCTATTGGGTTTATCGCGCAGTATGTGCTGAAACCGGCTCTAGGGGTGTTAATTGCAATGGCATTTGGCGTGCCTAGAATATTCTACGCTGGGTTTGTCCTCACCACTTGTGTTGCAGGGGCACAGCTGTCTAGTTATGCTAGTTTCTTGAGCAAAGGAGATGTGGCCTTGAGTATCCTCCTCACTAGCTCCACCACAATTGCCTCGGTGATCTTTACCCCTCTATTGACAGGCCTTTTAATTGGTTCTGTTGTTCCGGTCGATGCTGTTGCCATGGCAAAGTCAATATTGCAGGTTGTTCTTGTTCCAGTCACTATTGGCCTTGCGCTGAATACTTATGCCAAACCAGTTGTCACCATCCTTCGACCAGTGATGCCATTTGTTGCTATGATCTGTACTTCATTGTGCATTGGCAGTCCTCTTGCAATTAATCAAAGACAAATTCTGTCTATGGATGGTCTCCGATTGATATTTCCTGTTTTAGCATTTCATGCAGTGGCCTTTACTGTTGGATACTGGGTATCCAAAATTCCAAGTGTAAGGCAAGAAGAAGAAGTTAGCAGAACACTTTCATTATGCACAGGAATGCAAAGCTCCACCCTGGCGGGTCTCCTTGCAAGCCAGTTCCTTGGGAGCAGTCAAGCCGTTCCTCCAGCATGTTCGGTTGTTGCCATGGCCATCATGGGCCTTTTTCTTGCCTCTTTCTGGGGCACGGGTGCTCGAATTCGAGACCTTTCTTCCCTACTAATGCCTCAAACCAGTTCTACCATGAAGGCTTCGTAG

Protein Analysis

418

Amino Acids

43.58

Weight (kDa)

9.89

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 129 - 307 1.6e-33 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 754
AccII CGCG 1 cut(s) 488
AciI CCGC 3 cut(s) 190, 294, 1076
AclWI GGATC 1 cut(s) 407
AcoI YGGCCR 1 cut(s) 1146
AcsI RAATTY 3 cut(s) 912, 999, 1194
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 2 cut(s) 70, 868
AfiI CCNNNNNNNGG 5 cut(s) 170, 238, 392, 512, 1176
AjnI CCWGG 1 cut(s) 1071
AleI CACNNNNGTG 1 cut(s) 267
AluBI AGCT 5 cut(s) 45, 99, 598, 660, 1065
AluI AGCT 5 cut(s) 45, 99, 598, 660, 1065
Alw21I GWGCWC 1 cut(s) 1192
Alw26I GTCTC 5 cut(s) 76, 285, 935, 1085, 1194
AlwI GGATC 1 cut(s) 407
AoxI GGCC 6 cut(s) 636, 703, 790, 969, 1146, 1156
ApeKI GCWGC 3 cut(s) 187, 317, 332
ApoI RAATTY 3 cut(s) 912, 999, 1194
ArsI GACNNNNNNTTYG 4 cut(s) 414, 446, 807, 839
AseI ATTAAT 1 cut(s) 900
Asp700I GAANNNNTTC 2 cut(s) 205, 1039
AspLEI GCGC 2 cut(s) 490, 799
AspS9I GGNCC 1 cut(s) 1156
AsuHPI GGTGA 4 cut(s) 281, 565, 691, 817
AsuII TTCGAA 1 cut(s) 110
BaeGI GKGCMC 2 cut(s) 595, 1184
BaeI ACNNNNGTAYC 2 cut(s) 348, 381
BalI TGGCCA 1 cut(s) 1148
Bbv12I GWGCWC 1 cut(s) 1192
BbvI GCAGC 3 cut(s) 199, 304, 319
BccI CCATC 3 cut(s) 836, 920, 1157
BceAI ACGGC 3 cut(s) 148, 255, 1100
BcgI CGANNNNNNTGC 4 cut(s) 422, 456, 722, 756
BciT130I CCWGG 1 cut(s) 1073
BciVI GTATCC 4 cut(s) 376, 656, 977, 1003
BcoDI GTCTC 5 cut(s) 76, 285, 935, 1085, 1194
BfaI CTAG 5 cut(s) 512, 546, 603, 612, 657
BfuAI ACCTGC 1 cut(s) 754
BfuI GTATCC 4 cut(s) 376, 656, 977, 1003
BisI GCNGC 3 cut(s) 188, 318, 333
BlpI GCTNAGC 1 cut(s) 342
BlsI GCNGC 3 cut(s) 189, 319, 334
Bme1390I CCNGG 1 cut(s) 1073
BmgT120I GGNCC 1 cut(s) 1156
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 1073
BmrI ACTGGG 1 cut(s) 997
BmsI GCATC 2 cut(s) 724, 834
BmuI ACTGGG 1 cut(s) 997
BpmI CTGGAG 1 cut(s) 1107
Bpu1102I GCTNAGC 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 110
BpuEI CTTGAG 2 cut(s) 640, 661
BsaHI GRCGYC 1 cut(s) 158
BsaI GGTCTC 3 cut(s) 935, 1085, 1194
BsaJI CCNNGG 6 cut(s) 369, 675, 744, 1071, 1099, 1143
BsaWI WCCGGW 1 cut(s) 727
Bsc4I CCNNNNNNNGG 5 cut(s) 170, 238, 392, 512, 1176
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 6 cut(s) 779, 818, 839, 992, 1093, 1230
Bse3DI GCAATG 2 cut(s) 303, 534
BseBI CCWGG 1 cut(s) 1073
BseDI CCNNGG 6 cut(s) 369, 675, 744, 1071, 1099, 1143
BseGI GGATG 2 cut(s) 828, 931
BseLI CCNNNNNNNGG 5 cut(s) 170, 238, 392, 512, 1176
BseMI GCAATG 2 cut(s) 303, 534
BseMII CTCAG 2 cut(s) 296, 356
BseNI ACTGG 6 cut(s) 779, 818, 839, 992, 1093, 1230
BseRI GAGGAG 1 cut(s) 641
BseSI GKGCMC 2 cut(s) 595, 1184
BseXI GCAGC 3 cut(s) 199, 304, 319
BseYI CCCAGC 1 cut(s) 560
Bsh1236I CGCG 1 cut(s) 488
Bsh1285I CGRYCG 2 cut(s) 432, 732
BshFI GGCC 6 cut(s) 638, 705, 792, 971, 1148, 1158
BsiEI CGRYCG 2 cut(s) 432, 732
BsiHKAI GWGCWC 1 cut(s) 1192
BsiSI CCGG 2 cut(s) 506, 728
BslI CCNNNNNNNGG 5 cut(s) 170, 238, 392, 512, 1176
BsmAI GTCTC 5 cut(s) 76, 285, 935, 1085, 1194
BsmBI CGTCTC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 1062
BsnI GGCC 6 cut(s) 638, 705, 792, 971, 1148, 1158
Bso31I GGTCTC 3 cut(s) 935, 1085, 1194
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 3 cut(s) 595, 1184, 1192
Bsp143I GATC 3 cut(s) 399, 681, 861
Bsp1720I GCTNAGC 1 cut(s) 342
Bsp19I CCATGG 2 cut(s) 744, 1143
BspACI CCGC 3 cut(s) 190, 294, 1076
BspANI GGCC 6 cut(s) 638, 705, 792, 971, 1148, 1158
BspCNI CTCAG 2 cut(s) 295, 355
BspFNI CGCG 1 cut(s) 488
BspLI GGNNCC 1 cut(s) 256
BspMI ACCTGC 1 cut(s) 754
BspPI GGATC 1 cut(s) 407
BspQI GCTCTTC 1 cut(s) 106
BspT104I TTCGAA 1 cut(s) 110
BspTNI GGTCTC 3 cut(s) 935, 1085, 1194
BsrDI GCAATG 2 cut(s) 303, 534
BsrFI RCCGGY 1 cut(s) 505
BsrI ACTGG 6 cut(s) 779, 818, 839, 992, 1093, 1230
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 6 cut(s) 369, 675, 744, 1071, 1099, 1143
BssMI GATC 3 cut(s) 399, 681, 861
BssNI GRCGYC 1 cut(s) 158
BssT1I CCWWGG 4 cut(s) 369, 744, 1099, 1143
Bst2UI CCWGG 1 cut(s) 1073
Bst4CI ACNGT 2 cut(s) 266, 979
Bst6I CTCTTC 4 cut(s) 106, 107, 169, 211
BstACI GRCGYC 1 cut(s) 158
BstAPI GCANNNNNTGC 2 cut(s) 496, 962
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 3 cut(s) 348, 542, 1090
BstDEI CTNAG 2 cut(s) 282, 342
BstDSI CCRYGG 2 cut(s) 744, 1143
BstF5I GGATG 2 cut(s) 828, 931
BstFNI CGCG 1 cut(s) 488
BstHHI GCGC 2 cut(s) 490, 799
BstKTI GATC 3 cut(s) 402, 684, 864
BstMAI GTCTC 5 cut(s) 76, 285, 935, 1085, 1194
BstMBI GATC 3 cut(s) 399, 681, 861
BstMCI CGRYCG 2 cut(s) 432, 732
BstMWI GCNNNNNNNGC 6 cut(s) 323, 332, 338, 347, 496, 962
BstNI CCWGG 1 cut(s) 1073
BstNSI RCATGY 2 cut(s) 75, 1131
BstSCI CCNGG 1 cut(s) 1071
BstSLI GKGCMC 2 cut(s) 595, 1184
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 3 cut(s) 199, 304, 319
BstXI CCANNNNNNTGG 1 cut(s) 1100
BsuI GTATCC 4 cut(s) 376, 656, 977, 1003
BsuRI GGCC 6 cut(s) 638, 705, 792, 971, 1148, 1158
BtgI CCRYGG 2 cut(s) 744, 1143
BtsCI GGATG 2 cut(s) 828, 931
BtsI GCAGTG 2 cut(s) 321, 972
BtsIMutI CAGTG 4 cut(s) 271, 321, 846, 972
BveI ACCTGC 1 cut(s) 754
Cac8I GCNNGC 3 cut(s) 348, 542, 1090
CfoI GCGC 2 cut(s) 490, 799
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 1156
Csp6I GTAC 2 cut(s) 69, 867
CspCI CAANNNNNGTGG 6 cut(s) 310, 345, 565, 600, 652, 687
CviAII CATG 8 cut(s) 72, 403, 745, 962, 1128, 1144, 1153, 1240
CviQI GTAC 2 cut(s) 69, 867
DdeI CTNAG 2 cut(s) 282, 342
DpnI GATC 3 cut(s) 401, 683, 863
DpnII GATC 3 cut(s) 399, 681, 861
EaeI YGGCCR 1 cut(s) 1146
Eam1104I CTCTTC 4 cut(s) 106, 107, 169, 211
EarI CTCTTC 4 cut(s) 106, 107, 169, 211
Eco130I CCWWGG 4 cut(s) 369, 744, 1099, 1143
Eco147I AGGCCT 1 cut(s) 705
Eco31I GGTCTC 3 cut(s) 935, 1085, 1194
EcoRI GAATTC 1 cut(s) 1194
EcoRII CCWGG 1 cut(s) 1071
EcoT14I CCWWGG 4 cut(s) 369, 744, 1099, 1143
ErhI CCWWGG 4 cut(s) 369, 744, 1099, 1143
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 8 cut(s) 75, 406, 748, 965, 1131, 1147, 1156, 1243
FatI CATG 8 cut(s) 71, 402, 744, 961, 1127, 1143, 1152, 1239
FauI CCCGC 1 cut(s) 1069
Fnu4HI GCNGC 3 cut(s) 188, 318, 333
FokI GGATG 2 cut(s) 815, 938
Fsp4HI GCNGC 3 cut(s) 188, 318, 333
FspBI CTAG 5 cut(s) 512, 546, 603, 612, 657
GlaI GCGC 2 cut(s) 489, 798
GluI GCNGC 3 cut(s) 188, 318, 333
GsaI CCCAGC 1 cut(s) 564
GsuI CTGGAG 1 cut(s) 1107
HaeIII GGCC 6 cut(s) 638, 705, 792, 971, 1148, 1158
HapII CCGG 2 cut(s) 506, 728
HhaI GCGC 2 cut(s) 490, 799
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 8 cut(s) 75, 406, 748, 965, 1131, 1147, 1156, 1243
Hin6I GCGC 2 cut(s) 488, 797
HinP1I GCGC 2 cut(s) 488, 797
HinfI GANTC 3 cut(s) 275, 417, 468
HpaII CCGG 2 cut(s) 506, 728
HphI GGTGA 4 cut(s) 281, 565, 691, 817
Hpy166II GTNNAC 2 cut(s) 6, 357
Hpy188I TCNGA 2 cut(s) 125, 935
Hpy188III TCNNGA 2 cut(s) 619, 1199
Hpy8I GTNNAC 2 cut(s) 6, 357
Hpy99I CGWCG 2 cut(s) 160, 163
HpyAV CCTTC 4 cut(s) 31, 191, 842, 1237
HpyCH4III ACNGT 2 cut(s) 266, 979
HpyCH4IV ACGT 2 cut(s) 158, 353
HpyCH4V TGCA 9 cut(s) 527, 587, 763, 879, 896, 965, 1050, 1060, 1088
HpyF10VI GCNNNNNNNGC 6 cut(s) 323, 332, 338, 347, 496, 962
HpyF3I CTNAG 2 cut(s) 282, 342
HpySE526I ACGT 2 cut(s) 158, 353
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 8 cut(s) 75, 406, 748, 965, 1131, 1147, 1156, 1243
HspAI GCGC 2 cut(s) 488, 797
Kzo9I GATC 3 cut(s) 399, 681, 861
LguI GCTCTTC 1 cut(s) 106
LmnI GCTCC 4 cut(s) 254, 665, 1070, 1104
Lsp1109I GCAGC 3 cut(s) 199, 304, 319
LweI GCATC 2 cut(s) 724, 834
MaeI CTAG 5 cut(s) 512, 546, 603, 612, 657
MaeII ACGT 2 cut(s) 158, 353
MaeIII GTNAC 2 cut(s) 781, 823
MalI GATC 3 cut(s) 401, 683, 863
MboI GATC 3 cut(s) 399, 681, 861
MboII GAAGA 8 cut(s) 3, 93, 124, 186, 198, 1031, 1034, 1200
MfeI CAATTG 1 cut(s) 669
MhlI GDGCHC 3 cut(s) 595, 1184, 1192
MlsI TGGCCA 1 cut(s) 1148
MluCI AATT 7 cut(s) 522, 669, 711, 897, 912, 999, 1194
MluNI TGGCCA 1 cut(s) 1148
MlyI GAGTC 2 cut(s) 269, 462
MmeI TCCRAC 1 cut(s) 961
Mox20I TGGCCA 1 cut(s) 1148
MroXI GAANNNNTTC 2 cut(s) 205, 1039
MscI TGGCCA 1 cut(s) 1148
MseI TTAA 3 cut(s) 521, 710, 900
MslI CAYNNNNRTG 2 cut(s) 267, 1055
Msp20I TGGCCA 1 cut(s) 1148
MspA1I CMGCKG 2 cut(s) 190, 598
MspI CCGG 2 cut(s) 506, 728
MspR9I CCNGG 1 cut(s) 1073
MunI CAATTG 1 cut(s) 669
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 1 cut(s) 1073
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 6 cut(s) 323, 332, 338, 347, 496, 962
NcoI CCATGG 2 cut(s) 744, 1143
NdeII GATC 3 cut(s) 399, 681, 861
NlaIII CATG 8 cut(s) 75, 406, 748, 965, 1131, 1147, 1156, 1243
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 781, 823
NspI RCATGY 2 cut(s) 75, 1131
NspV TTCGAA 1 cut(s) 110
OliI CACNNNNGTG 1 cut(s) 267
PceI AGGCCT 1 cut(s) 705
PciSI GCTCTTC 1 cut(s) 106
PcsI WCGNNNNNNNCGW 2 cut(s) 161, 215
PctI GAATGC 1 cut(s) 1062
PdmI GAANNNNTTC 2 cut(s) 205, 1039
PfeI GAWTC 1 cut(s) 417
PkrI GCNGC 3 cut(s) 189, 319, 334
PleI GAGTC 2 cut(s) 269, 462
PpsI GAGTC 2 cut(s) 269, 462
PshBI ATTAAT 1 cut(s) 900
Psp6I CCWGG 1 cut(s) 1071
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 1071
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 1156
PvuII CAGCTG 1 cut(s) 598
RsaI GTAC 2 cut(s) 70, 868
RsaNI GTAC 2 cut(s) 69, 867
RseI CAYNNNNRTG 2 cut(s) 267, 1055
SapI GCTCTTC 1 cut(s) 106
SaqAI TTAA 3 cut(s) 521, 710, 900
SatI GCNGC 3 cut(s) 188, 318, 333
Sau3AI GATC 3 cut(s) 399, 681, 861
Sau96I GGNCC 1 cut(s) 1156
SchI GAGTC 2 cut(s) 269, 462
ScrFI CCNGG 1 cut(s) 1073
SduI GDGCHC 3 cut(s) 595, 1184, 1192
SfaNI GCATC 2 cut(s) 724, 834
SfuI TTCGAA 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 267, 1055
SmlI CTYRAG 2 cut(s) 619, 640
SmoI CTYRAG 2 cut(s) 619, 640
Sse9I AATT 7 cut(s) 522, 669, 711, 897, 912, 999, 1194
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 3 cut(s) 190, 294, 1076
SspI AATATT 2 cut(s) 552, 759
SspMI CTAG 5 cut(s) 512, 546, 603, 612, 657
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 1 cut(s) 1071
StyI CCWWGG 4 cut(s) 369, 744, 1099, 1143
TaaI ACNGT 2 cut(s) 266, 979
TaiI ACGT 2 cut(s) 161, 356
TaqI TCGA 8 cut(s) 110, 140, 155, 432, 732, 835, 1192, 1198
TaqII GACCGA 1 cut(s) 418
TasI AATT 7 cut(s) 522, 669, 711, 897, 912, 999, 1194
TatI WGTACW 2 cut(s) 68, 866
TfiI GAWTC 1 cut(s) 417
Tru1I TTAA 3 cut(s) 521, 710, 900
Tru9I TTAA 3 cut(s) 521, 710, 900
TscAI CASTG 4 cut(s) 271, 328, 846, 972
TseFI GTSAC 2 cut(s) 781, 823
TseI GCWGC 3 cut(s) 187, 317, 332
Tsp45I GTSAC 2 cut(s) 781, 823
TspDTI ATGAA 5 cut(s) 13, 93, 861, 950, 1032
TspRI CASTG 4 cut(s) 271, 328, 846, 972
VspI ATTAAT 1 cut(s) 900
XapI RAATTY 3 cut(s) 912, 999, 1194
XceI RCATGY 2 cut(s) 75, 1131
XcmI CCANNNNNNNNNTGG 1 cut(s) 1150
XmnI GAANNNNTTC 2 cut(s) 205, 1039
XspI CTAG 5 cut(s) 512, 546, 603, 612, 657
ZraI GACGTC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.