MD05G1158500.v1.1

sodium metabolite cotransporter BASS3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008339
Physical Location & Seq
Forward (+)
28760718 .. 28763592
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1158500.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGTCCACCACTCCTATCTCCTATCCAATAACCTCAAGACCCTTCCTTTCTCTCACACCCGCCACCGCAGCCTCAAAACTCTTTGCAAACCCGAATTACCACACCCAAAGCTCTCTCTTTTCCTCCTCTTCGTCTTCCTCCAGTCATTTCCCCAAAAGGGTATTCAGAAATGGGGTTTCTCTGTCGATAAGAGCTTGCTCGACGTCGCCGTTTATCGGCAGAGTCGGGTCGCAGAGGAGGGAAGGCAACGCGTCGATGCTGTCGTTTGGGATAAACCCATCAGCTCAAATATCCAAAGGGGCTTCCGATGGCTCGTCGTCTCAGGTCTTATCTGCAATGCTTCCTTTCGTCGTTGCTGCTACCGCCATTGCTGCTCTGGCTCAGCCGTCAACGTTCACTTGGGTATCCAAGGATTTATATGCCCCTGCTCTTGGTGGGATCATGCTGTCAATTGGAATCAAACTTTCGATTGATGATTTTGCTCTTGCATTCAAAAGACCTTTACCACTCTCTGTTGGGTTTATCGCGCAGTATGTGCTGAAACCGCTACTTGGGGTACTAATTGCAAACGCATTTGGCGTGCCGAGGATGTTCTACGCTGGCTTTGTTCTCACCGCTTGTGTTGCAGGGGCTCAGCTATCTAGCTACGCTAGTTTCTTGAGCAAAGGAGATGTGGCCTTGAGCATTCTCCTTACCAGCACCACCACCATTGCTTCCGTGATTGCAACGCCTTTACTAACTGGCCTTTTAATCGGTTCTGTTGTTCCGGTTGATGCTGTTGCCATGTCAAAGTCAATATTGCAGGTGGTTCTTGTTCCAGTTACAATTGGTCTTGTGCTCAATACTTATGCAAAACCGGTAGTCAATTTCCTTCGACCAGTGATGCCAGTTGTTGCTATGATTTGCACTTCATTGTGCATTGGTAGTCCTCTTGCAATTAATCGGAGTCAAATTCTATCCGTAGAAGGTTTCCGGTTGATATTTCCAGTCATAGCATTTCACGCTGTGGCCTTTACACTTGGATACTGGGTATCCAAATCTCCAGGTTTGAGGCAAGAAGAAGAAGTTAGCAGGACACTTTCCTTATGCACAGGAATGCAGAGTTCCACTTTGGCAGGGCTCCTTGCAAGCCAGTTCCTTGGGAGCAGCCAGGCCGTTCCTCCAGCATGTTCAGTTGTTGCCATGGCCATCATGGGCCTTTTTCTTGCATCTTTCTGGGGCACCGGCTCTCGAATCAGAGACCTGCTGTCCCTACTAACGCCTCAAACAGGTTCTCCAATGAAGGCGTAG

Protein Analysis

430

Amino Acids

44.88

Weight (kDa)

9.95

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 142 - 320 2.5e-34 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 793
AatII GACGTC 1 cut(s) 206
Acc36I ACCTGC 2 cut(s) 793, 1251
AccB1I GGYRCC 1 cut(s) 1220
AccII CGCG 2 cut(s) 251, 527
AciI CCGC 5 cut(s) 60, 66, 363, 545, 615
AclI AACGTT 1 cut(s) 392
AclWI GGATC 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 1185
AcsI RAATTY 1 cut(s) 951
AcyI GRCGYC 1 cut(s) 203
AdeI CACNNNGTG 1 cut(s) 1006
AfaI GTAC 1 cut(s) 558
AfiI CCNNNNNNNGG 5 cut(s) 157, 215, 431, 551, 1268
AflIII ACRYGT 1 cut(s) 249
AgeI ACCGGT 1 cut(s) 856
AgsI TTSAA 1 cut(s) 493
AhdI GACNNNNNGTC 1 cut(s) 1246
AjnI CCWGG 2 cut(s) 1042, 1149
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 5 cut(s) 111, 194, 284, 637, 645
AluI AGCT 5 cut(s) 111, 194, 284, 637, 645
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 2 cut(s) 324, 1233
AlwI GGATC 1 cut(s) 446
AoxI GGCC 6 cut(s) 675, 742, 1008, 1152, 1185, 1195
ApeKI GCWGC 4 cut(s) 68, 356, 371, 1146
ApoI RAATTY 1 cut(s) 951
AseI ATTAAT 1 cut(s) 939
AsiGI ACCGGT 1 cut(s) 856
AspLEI GCGC 1 cut(s) 529
AspS9I GGNCC 1 cut(s) 1195
AsuHPI GGTGA 1 cut(s) 604
BaeGI GKGCMC 1 cut(s) 1223
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BalI TGGCCA 1 cut(s) 1187
BanI GGYRCC 1 cut(s) 1220
BanII GRGCYC 2 cut(s) 634, 1122
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 4 cut(s) 80, 343, 358, 1158
BccI CCATC 3 cut(s) 286, 302, 1196
BceAI ACGGC 3 cut(s) 193, 370, 1139
BciT130I CCWGG 2 cut(s) 1044, 1151
BciVI GTATCC 3 cut(s) 415, 1016, 1042
BcoDI GTCTC 2 cut(s) 324, 1233
BfaI CTAG 2 cut(s) 642, 651
BfuAI ACCTGC 2 cut(s) 793, 1251
BfuI GTATCC 3 cut(s) 415, 1016, 1042
BisI GCNGC 4 cut(s) 69, 357, 372, 1147
BlpI GCTNAGC 2 cut(s) 381, 633
BlsI GCNGC 4 cut(s) 70, 358, 373, 1148
Bme1390I CCNGG 2 cut(s) 1044, 1151
BmeRI GACNNNNNGTC 1 cut(s) 1246
BmgT120I GGNCC 1 cut(s) 1195
BmiI GGNNCC 2 cut(s) 1121, 1222
BmrFI CCNGG 2 cut(s) 1044, 1151
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 4 cut(s) 246, 763, 873, 1217
BmuI ACTGGG 1 cut(s) 1036
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 3 cut(s) 124, 1026, 1146
Bpu1102I GCTNAGC 2 cut(s) 381, 633
BpuEI CTTGAG 3 cut(s) 19, 679, 700
BsaHI GRCGYC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 1233
BsaJI CCNNGG 4 cut(s) 408, 584, 1138, 1182
BsaWI WCCGGW 3 cut(s) 766, 856, 972
BsaXI ACNNNNNCTCC 2 cut(s) 1258, 1288
Bsc4I CCNNNNNNNGG 5 cut(s) 157, 215, 431, 551, 1268
Bse118I RCCGGY 2 cut(s) 856, 1223
Bse1I ACTGG 8 cut(s) 141, 745, 818, 878, 887, 986, 1031, 1132
Bse3DI GCAATG 3 cut(s) 342, 366, 708
BseBI CCWGG 2 cut(s) 1044, 1151
BseDI CCNNGG 4 cut(s) 408, 584, 1138, 1182
BseGI GGATG 1 cut(s) 594
BseLI CCNNNNNNNGG 5 cut(s) 157, 215, 431, 551, 1268
BseMI GCAATG 3 cut(s) 342, 366, 708
BseMII CTCAG 3 cut(s) 335, 395, 647
BseNI ACTGG 8 cut(s) 141, 745, 818, 878, 887, 986, 1031, 1132
BseRI GAGGAG 2 cut(s) 115, 250
BseSI GKGCMC 1 cut(s) 1223
BseXI GCAGC 4 cut(s) 80, 343, 358, 1158
Bsh1236I CGCG 2 cut(s) 251, 527
BshFI GGCC 6 cut(s) 677, 744, 1010, 1154, 1187, 1197
BshNI GGYRCC 1 cut(s) 1220
BshTI ACCGGT 1 cut(s) 856
BsiHKAI GWGCWC 1 cut(s) 840
BsiSI CCGG 4 cut(s) 767, 857, 973, 1224
BslFI GGGAC 1 cut(s) 1234
BslI CCNNNNNNNGG 5 cut(s) 157, 215, 431, 551, 1268
BsmAI GTCTC 2 cut(s) 324, 1233
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 1234
BsmI GAATGC 3 cut(s) 488, 684, 1101
BsnI GGCC 6 cut(s) 677, 744, 1010, 1154, 1187, 1197
Bso31I GGTCTC 1 cut(s) 1233
Bsp1286I GDGCHC 4 cut(s) 634, 840, 1122, 1223
Bsp143I GATC 1 cut(s) 438
Bsp1720I GCTNAGC 2 cut(s) 381, 633
Bsp19I CCATGG 1 cut(s) 1182
BspACI CCGC 5 cut(s) 60, 66, 363, 545, 615
BspANI GGCC 6 cut(s) 677, 744, 1010, 1154, 1187, 1197
BspCNI CTCAG 3 cut(s) 334, 394, 646
BspFNI CGCG 2 cut(s) 251, 527
BspLI GGNNCC 2 cut(s) 1121, 1222
BspMI ACCTGC 2 cut(s) 793, 1251
BspPI GGATC 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 1220
BspTNI GGTCTC 1 cut(s) 1233
BsrDI GCAATG 3 cut(s) 342, 366, 708
BsrFI RCCGGY 2 cut(s) 856, 1223
BsrI ACTGG 8 cut(s) 141, 745, 818, 878, 887, 986, 1031, 1132
BssAI RCCGGY 2 cut(s) 856, 1223
BssECI CCNNGG 4 cut(s) 408, 584, 1138, 1182
BssMI GATC 1 cut(s) 438
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 3 cut(s) 408, 1138, 1182
Bst2UI CCWGG 2 cut(s) 1044, 1151
Bst6I CTCTTC 1 cut(s) 133
BstACI GRCGYC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 535
BstC8I GCNNGC 4 cut(s) 196, 581, 601, 1129
BstDEI CTNAG 3 cut(s) 321, 381, 633
BstDSI CCRYGG 1 cut(s) 1182
BstENI CCTNNNNNAGG 1 cut(s) 1266
BstF5I GGATG 1 cut(s) 594
BstFNI CGCG 2 cut(s) 251, 527
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 1 cut(s) 441
BstMAI GTCTC 2 cut(s) 324, 1233
BstMBI GATC 1 cut(s) 438
BstMWI GCNNNNNNNGC 8 cut(s) 68, 362, 371, 377, 535, 544, 623, 1001
BstNI CCWGG 2 cut(s) 1044, 1151
BstNSI RCATGY 1 cut(s) 1170
BstSCI CCNGG 2 cut(s) 1042, 1149
BstSLI GKGCMC 1 cut(s) 1223
BstUI CGCG 2 cut(s) 251, 527
BstV1I GCAGC 4 cut(s) 80, 343, 358, 1158
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 1139
BsuI GTATCC 3 cut(s) 415, 1016, 1042
BsuRI GGCC 6 cut(s) 677, 744, 1010, 1154, 1187, 1197
BtgI CCRYGG 1 cut(s) 1182
BtsCI GGATG 1 cut(s) 594
BtsIMutI CAGTG 1 cut(s) 885
BveI ACCTGC 2 cut(s) 793, 1251
Cac8I GCNNGC 4 cut(s) 196, 581, 601, 1129
CfoI GCGC 1 cut(s) 529
Cfr10I RCCGGY 2 cut(s) 856, 1223
Cfr13I GGNCC 1 cut(s) 1195
CseI GACGC 1 cut(s) 240
Csp6I GTAC 1 cut(s) 557
CspAI ACCGGT 1 cut(s) 856
CspCI CAANNNNNGTGG 2 cut(s) 691, 726
CviAII CATG 5 cut(s) 442, 784, 1167, 1183, 1192
CviQI GTAC 1 cut(s) 557
DdeI CTNAG 3 cut(s) 321, 381, 633
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
DraIII CACNNNGTG 1 cut(s) 1006
DriI GACNNNNNGTC 1 cut(s) 1246
EaeI YGGCCR 1 cut(s) 1185
Eam1104I CTCTTC 1 cut(s) 133
Eam1105I GACNNNNNGTC 1 cut(s) 1246
EarI CTCTTC 1 cut(s) 133
Eco130I CCWWGG 3 cut(s) 408, 1138, 1182
Eco24I GRGCYC 2 cut(s) 634, 1122
Eco31I GGTCTC 1 cut(s) 1233
EcoNI CCTNNNNNAGG 1 cut(s) 1266
EcoRII CCWGG 2 cut(s) 1042, 1149
EcoT14I CCWWGG 3 cut(s) 408, 1138, 1182
EcoT38I GRGCYC 2 cut(s) 634, 1122
ErhI CCWWGG 3 cut(s) 408, 1138, 1182
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 5 cut(s) 445, 787, 1170, 1186, 1195
FaqI GGGAC 1 cut(s) 1234
FatI CATG 5 cut(s) 441, 783, 1166, 1182, 1191
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 4 cut(s) 69, 357, 372, 1147
FokI GGATG 1 cut(s) 601
FriOI GRGCYC 2 cut(s) 634, 1122
Fsp4HI GCNGC 4 cut(s) 69, 357, 372, 1147
FspBI CTAG 2 cut(s) 642, 651
GlaI GCGC 1 cut(s) 528
GluI GCNGC 4 cut(s) 69, 357, 372, 1147
GsuI CTGGAG 3 cut(s) 124, 1026, 1146
HaeIII GGCC 6 cut(s) 677, 744, 1010, 1154, 1187, 1197
HapII CCGG 4 cut(s) 767, 857, 973, 1224
HgaI GACGC 1 cut(s) 240
HhaI GCGC 1 cut(s) 529
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 5 cut(s) 445, 787, 1170, 1186, 1195
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HinfI GANTC 4 cut(s) 222, 456, 946, 1233
HpaII CCGG 4 cut(s) 767, 857, 973, 1224
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 3 cut(s) 6, 390, 396
Hpy188I TCNGA 4 cut(s) 167, 307, 945, 1238
Hpy188III TCNNGA 3 cut(s) 36, 658, 1230
Hpy8I GTNNAC 3 cut(s) 6, 390, 396
Hpy99I CGWCG 5 cut(s) 205, 208, 256, 319, 353
HpyAV CCTTC 5 cut(s) 52, 236, 881, 959, 1276
HpyCH4IV ACGT 2 cut(s) 203, 392
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 362, 371, 377, 535, 544, 623, 1001
HpyF3I CTNAG 3 cut(s) 321, 381, 633
HpySE526I ACGT 2 cut(s) 203, 392
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 5 cut(s) 445, 787, 1170, 1186, 1195
HspAI GCGC 1 cut(s) 527
Kzo9I GATC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 1125, 1143
Lsp1109I GCAGC 4 cut(s) 80, 343, 358, 1158
LweI GCATC 4 cut(s) 246, 763, 873, 1217
MaeI CTAG 2 cut(s) 642, 651
MaeII ACGT 2 cut(s) 203, 392
MaeIII GTNAC 1 cut(s) 820
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 4 cut(s) 120, 126, 1070, 1073
MfeI CAATTG 2 cut(s) 450, 825
MhlI GDGCHC 4 cut(s) 634, 840, 1122, 1223
MlsI TGGCCA 1 cut(s) 1187
MluCI AATT 7 cut(s) 94, 450, 561, 825, 865, 936, 951
MluI ACGCGT 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 1187
MlyI GAGTC 2 cut(s) 231, 955
Mox20I TGGCCA 1 cut(s) 1187
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 2 cut(s) 749, 939
MslI CAYNNNNRTG 1 cut(s) 1094
Msp20I TGGCCA 1 cut(s) 1187
MspI CCGG 4 cut(s) 767, 857, 973, 1224
MspR9I CCNGG 2 cut(s) 1044, 1151
MunI CAATTG 2 cut(s) 450, 825
Mva1269I GAATGC 3 cut(s) 488, 684, 1101
MvaI CCWGG 2 cut(s) 1044, 1151
MvnI CGCG 2 cut(s) 251, 527
MwoI GCNNNNNNNGC 8 cut(s) 68, 362, 371, 377, 535, 544, 623, 1001
NcoI CCATGG 1 cut(s) 1182
NdeII GATC 1 cut(s) 438
NlaIII CATG 5 cut(s) 445, 787, 1170, 1186, 1195
NlaIV GGNNCC 2 cut(s) 1121, 1222
NmeAIII GCCGAG 1 cut(s) 609
NspI RCATGY 1 cut(s) 1170
PaqCI CACCTGC 1 cut(s) 793
PcsI WCGNNNNNNNCGW 3 cut(s) 206, 260, 576
PctI GAATGC 3 cut(s) 488, 684, 1101
PfeI GAWTC 2 cut(s) 456, 1233
PinAI ACCGGT 1 cut(s) 856
PkrI GCNGC 4 cut(s) 70, 358, 373, 1148
PleI GAGTC 2 cut(s) 230, 954
PpsI GAGTC 2 cut(s) 230, 954
PshBI ATTAAT 1 cut(s) 939
Psp1406I AACGTT 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 1042, 1149
PspGI CCWGG 2 cut(s) 1042, 1149
PspN4I GGNNCC 2 cut(s) 1121, 1222
PspPI GGNCC 1 cut(s) 1195
RsaI GTAC 1 cut(s) 558
RsaNI GTAC 1 cut(s) 557
RseI CAYNNNNRTG 1 cut(s) 1094
SaqAI TTAA 2 cut(s) 749, 939
SatI GCNGC 4 cut(s) 69, 357, 372, 1147
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 1 cut(s) 1195
SchI GAGTC 2 cut(s) 231, 955
ScrFI CCNGG 2 cut(s) 1044, 1151
SduI GDGCHC 4 cut(s) 634, 840, 1122, 1223
SfaNI GCATC 4 cut(s) 246, 763, 873, 1217
SmiMI CAYNNNNRTG 1 cut(s) 1094
SmlI CTYRAG 3 cut(s) 34, 658, 679
SmoI CTYRAG 3 cut(s) 34, 658, 679
Sse9I AATT 7 cut(s) 94, 450, 561, 825, 865, 936, 951
SsiI CCGC 5 cut(s) 60, 66, 363, 545, 615
SspI AATATT 1 cut(s) 798
SspMI CTAG 2 cut(s) 642, 651
StyD4I CCNGG 2 cut(s) 1042, 1149
StyI CCWWGG 3 cut(s) 408, 1138, 1182
TaiI ACGT 2 cut(s) 206, 395
TaqI TCGA 6 cut(s) 185, 200, 254, 467, 874, 1231
TasI AATT 7 cut(s) 94, 450, 561, 825, 865, 936, 951
TfiI GAWTC 2 cut(s) 456, 1233
Tru1I TTAA 2 cut(s) 749, 939
Tru9I TTAA 2 cut(s) 749, 939
TscAI CASTG 1 cut(s) 885
TseI GCWGC 4 cut(s) 68, 356, 371, 1146
TspDTI ATGAA 1 cut(s) 900
TspGWI ACGGA 2 cut(s) 706, 949
TspRI CASTG 1 cut(s) 885
VspI ATTAAT 1 cut(s) 939
XagI CCTNNNNNAGG 1 cut(s) 1266
XapI RAATTY 1 cut(s) 951
XceI RCATGY 1 cut(s) 1170
XcmI CCANNNNNNNNNTGG 2 cut(s) 373, 1189
XspI CTAG 2 cut(s) 642, 651
ZraI GACGTC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.