Rroxscaffold_7G00186080

sodium metabolite cotransporter BASS3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008339
Physical Location & Seq
Reverse (-)
25497056 .. 25501705
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00186080.1

Sequence Viewer

Length: 1260 bp
ATGTCCACTTCTTCCATACCCTTCATTTCTCTCACACCCAACAGCTCAAAACTTCTGCTAAAGCCAAACACATGCACACCGAGACTGAGACTATCAAGCTCTTCATTTTCGTGTGGTTCGTTCTCGAAGAGGGGGTTCAGAAATGGGGGTCTGACGATAAAGGGTTGCTCGACGTCGCCGTTTATAGGAAGAGTTGGGTCGCAGCGGAGGGAAGGGAACTCGTCGTTGCTTTCGTTTGGGATAAACCCACCAACGGCTATGTCCAAAGGAGCCGAACAGAGTGGTGAGGACTCGTCTCAGGTCTTATCCGCAATGCTTCCATTTGTGGTTGCTGCCACTGCTATTGCTGCTTTGGCTCAGCCTGCCACGTTTACTTGGGTATCCAAGGATTTATACGCCCCTGCTCTTGGTGGGATCATGCTCTCCATTGGAATCAAACTTTCGGTCGATGATTTTGCTCTTGCTGTCAAAAGACCTTTACCACTCTCTATTGGGTTTATCGCACAGTATGTGCTGAAACCGGCTCTAGGGGTGTTAATTGCAATGGCATTTGGCGTGCCTAGAATATTCTACGCTGGGTTTGTCCTCACCACTTGTGTTGCAGGGGCACAGCTGTCTAGTTATGCTAGTTTCTTGAGCAAAGGAGATGTGGCCTTGAGTATCCTCCTCACTAGCTCCACCACAATTGCCTCGGTGATCTTTACCCCTCTACTGACAGGCCTTTTAATTGGTTCTGTTGTTCCGGTTGATGCTGTTGCCATGGCAAAGTCAATATTGCAGGTTGTTCTTGTTCCAGTCACTATTGGCCTTGCGCTGAATACTTATGCCAAACCAGTTGTCACCATCCTTCGACCAGTGATGCCATTTGTTGCTATGATCTGTACTTCATTGTGCATTGGCAGTCCTCTTGCAATTAATCAAAGACAAATTCTGTCTATGGATGGTCTCCGATTGATATTTCCTGTTTTAGCATTTCATGCAGTGGCCTTTACTGTTGGATACTGGGTATCCAAAATTCCAAGTGTAAGGCAAGAAGAAGAAGTTAGCAGAACACTTTCATTATGCACAGGAATGCAAAGCTCCACCCTGGCTGGTCTCCTTGCAAGCCAGTTCCTTGGGAGCAGTCAAGCCGTTCCTCCAGCATGTTCGGTTGTTGCCATGGCCATCATGGGCCTTTTTCTTGCCTCTTTCTGGGGCACGGGTGCTCGGTCGAGACCTTTCTTCCCTACTAATGCCTCAAACCAGTCAATGGATCACTGA

Protein Analysis

419

Amino Acids

43.89

Weight (kDa)

9.77

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 134 - 312 1.1e-33 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 176
Acc36I ACCTGC 1 cut(s) 769
AccB7I CCANNNNNTGG 1 cut(s) 1249
AciI CCGC 2 cut(s) 205, 309
AclWI GGATC 1 cut(s) 422
AcoI YGGCCR 1 cut(s) 1161
AcsI RAATTY 2 cut(s) 927, 1014
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 1 cut(s) 883
AfiI CCNNNNNNNGG 6 cut(s) 185, 253, 407, 527, 1191, 1249
AjnI CCWGG 1 cut(s) 1086
AluBI AGCT 5 cut(s) 45, 99, 613, 675, 1080
AluI AGCT 5 cut(s) 45, 99, 613, 675, 1080
Alw21I GWGCWC 1 cut(s) 1207
Alw26I GTCTC 6 cut(s) 76, 82, 300, 950, 1100, 1207
AlwI GGATC 1 cut(s) 422
AoxI GGCC 6 cut(s) 651, 718, 805, 984, 1161, 1171
ApeKI GCWGC 3 cut(s) 202, 332, 347
ApoI RAATTY 2 cut(s) 927, 1014
ArsI GACNNNNNNTTYG 4 cut(s) 429, 461, 822, 854
AseI ATTAAT 1 cut(s) 915
Asp700I GAANNNNTTC 1 cut(s) 1054
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 1 cut(s) 1171
AsuHPI GGTGA 4 cut(s) 296, 580, 706, 832
BaeGI GKGCMC 2 cut(s) 610, 1199
BaeI ACNNNNGTAYC 2 cut(s) 363, 396
BalI TGGCCA 1 cut(s) 1163
Bbv12I GWGCWC 1 cut(s) 1207
BbvI GCAGC 3 cut(s) 214, 319, 334
BccI CCATC 3 cut(s) 851, 935, 1172
BceAI ACGGC 3 cut(s) 163, 270, 1115
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 1 cut(s) 1088
BciVI GTATCC 4 cut(s) 391, 671, 992, 1018
BcoDI GTCTC 6 cut(s) 76, 82, 300, 950, 1100, 1207
BfaI CTAG 5 cut(s) 527, 561, 618, 627, 672
BfuAI ACCTGC 1 cut(s) 769
BfuI GTATCC 4 cut(s) 391, 671, 992, 1018
BisI GCNGC 3 cut(s) 203, 333, 348
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 3 cut(s) 204, 334, 349
Bme1390I CCNGG 1 cut(s) 1088
BmgT120I GGNCC 1 cut(s) 1171
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 1088
BmrI ACTGGG 1 cut(s) 1012
BmsI GCATC 2 cut(s) 739, 849
BmuI ACTGGG 1 cut(s) 1012
BpmI CTGGAG 1 cut(s) 1122
Bpu1102I GCTNAGC 1 cut(s) 357
BpuEI CTTGAG 2 cut(s) 655, 676
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 3 cut(s) 950, 1100, 1207
BsaJI CCNNGG 6 cut(s) 384, 690, 759, 1086, 1114, 1158
BsaWI WCCGGW 1 cut(s) 742
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 253, 407, 527, 1191, 1249
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 6 cut(s) 794, 833, 854, 1007, 1108, 1243
Bse3DI GCAATG 2 cut(s) 318, 549
BseBI CCWGG 1 cut(s) 1088
BseDI CCNNGG 6 cut(s) 384, 690, 759, 1086, 1114, 1158
BseGI GGATG 2 cut(s) 843, 946
BseLI CCNNNNNNNGG 6 cut(s) 185, 253, 407, 527, 1191, 1249
BseMI GCAATG 2 cut(s) 318, 549
BseMII CTCAG 3 cut(s) 77, 311, 371
BseNI ACTGG 6 cut(s) 794, 833, 854, 1007, 1108, 1243
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 2 cut(s) 610, 1199
BseXI GCAGC 3 cut(s) 214, 319, 334
BseYI CCCAGC 1 cut(s) 575
Bsh1285I CGRYCG 2 cut(s) 447, 1211
BshFI GGCC 6 cut(s) 653, 720, 807, 986, 1163, 1173
BsiEI CGRYCG 2 cut(s) 447, 1211
BsiHKAI GWGCWC 1 cut(s) 1207
BsiSI CCGG 2 cut(s) 521, 743
BslI CCNNNNNNNGG 6 cut(s) 185, 253, 407, 527, 1191, 1249
BsmAI GTCTC 6 cut(s) 76, 82, 300, 950, 1100, 1207
BsmBI CGTCTC 1 cut(s) 300
BsmI GAATGC 1 cut(s) 1077
BsnI GGCC 6 cut(s) 653, 720, 807, 986, 1163, 1173
Bso31I GGTCTC 3 cut(s) 950, 1100, 1207
Bsp1286I GDGCHC 3 cut(s) 610, 1199, 1207
Bsp143I GATC 4 cut(s) 414, 696, 876, 1252
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 2 cut(s) 759, 1158
BspACI CCGC 2 cut(s) 205, 309
BspANI GGCC 6 cut(s) 653, 720, 807, 986, 1163, 1173
BspCNI CTCAG 3 cut(s) 78, 310, 370
BspLI GGNNCC 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 769
BspPI GGATC 1 cut(s) 422
BspQI GCTCTTC 1 cut(s) 106
BspTNI GGTCTC 3 cut(s) 950, 1100, 1207
BsrDI GCAATG 2 cut(s) 318, 549
BsrFI RCCGGY 1 cut(s) 520
BsrI ACTGG 6 cut(s) 794, 833, 854, 1007, 1108, 1243
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 6 cut(s) 384, 690, 759, 1086, 1114, 1158
BssMI GATC 4 cut(s) 414, 696, 876, 1252
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 4 cut(s) 384, 759, 1114, 1158
Bst2UI CCWGG 1 cut(s) 1088
Bst4CI ACNGT 2 cut(s) 507, 994
Bst6I CTCTTC 3 cut(s) 106, 122, 184
BstACI GRCGYC 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 977
BstC8I GCNNGC 3 cut(s) 363, 557, 1105
BstDEI CTNAG 3 cut(s) 86, 297, 357
BstDSI CCRYGG 2 cut(s) 759, 1158
BstF5I GGATG 2 cut(s) 843, 946
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 4 cut(s) 417, 699, 879, 1255
BstMAI GTCTC 6 cut(s) 76, 82, 300, 950, 1100, 1207
BstMBI GATC 4 cut(s) 414, 696, 876, 1252
BstMCI CGRYCG 2 cut(s) 447, 1211
BstMWI GCNNNNNNNGC 5 cut(s) 338, 347, 353, 362, 977
BstNI CCWGG 1 cut(s) 1088
BstNSI RCATGY 2 cut(s) 75, 1146
BstSCI CCNGG 1 cut(s) 1086
BstSLI GKGCMC 2 cut(s) 610, 1199
BstV1I GCAGC 3 cut(s) 214, 319, 334
BstXI CCANNNNNNTGG 1 cut(s) 1115
BsuI GTATCC 4 cut(s) 391, 671, 992, 1018
BsuRI GGCC 6 cut(s) 653, 720, 807, 986, 1163, 1173
BtgI CCRYGG 2 cut(s) 759, 1158
BtsCI GGATG 2 cut(s) 843, 946
BtsI GCAGTG 2 cut(s) 336, 987
BtsIMutI CAGTG 4 cut(s) 336, 861, 987, 1255
BveI ACCTGC 1 cut(s) 769
Cac8I GCNNGC 3 cut(s) 363, 557, 1105
CfoI GCGC 1 cut(s) 814
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 1171
Csp6I GTAC 1 cut(s) 882
CspCI CAANNNNNGTGG 6 cut(s) 325, 360, 580, 615, 667, 702
CviAII CATG 7 cut(s) 72, 418, 760, 977, 1143, 1159, 1168
CviQI GTAC 1 cut(s) 882
DdeI CTNAG 3 cut(s) 86, 297, 357
DpnI GATC 4 cut(s) 416, 698, 878, 1254
DpnII GATC 4 cut(s) 414, 696, 876, 1252
EaeI YGGCCR 1 cut(s) 1161
Eam1104I CTCTTC 3 cut(s) 106, 122, 184
EarI CTCTTC 3 cut(s) 106, 122, 184
Eco130I CCWWGG 4 cut(s) 384, 759, 1114, 1158
Eco147I AGGCCT 1 cut(s) 720
Eco31I GGTCTC 3 cut(s) 950, 1100, 1207
EcoRII CCWGG 1 cut(s) 1086
EcoT14I CCWWGG 4 cut(s) 384, 759, 1114, 1158
ErhI CCWWGG 4 cut(s) 384, 759, 1114, 1158
Esp3I CGTCTC 1 cut(s) 300
FaeI CATG 7 cut(s) 75, 421, 763, 980, 1146, 1162, 1171
FatI CATG 7 cut(s) 71, 417, 759, 976, 1142, 1158, 1167
Fnu4HI GCNGC 3 cut(s) 203, 333, 348
FokI GGATG 2 cut(s) 830, 953
Fsp4HI GCNGC 3 cut(s) 203, 333, 348
FspBI CTAG 5 cut(s) 527, 561, 618, 627, 672
GlaI GCGC 1 cut(s) 813
GluI GCNGC 3 cut(s) 203, 333, 348
GsaI CCCAGC 1 cut(s) 579
GsuI CTGGAG 1 cut(s) 1122
HaeIII GGCC 6 cut(s) 653, 720, 807, 986, 1163, 1173
HapII CCGG 2 cut(s) 521, 743
HhaI GCGC 1 cut(s) 814
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 7 cut(s) 75, 421, 763, 980, 1146, 1162, 1171
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HinfI GANTC 2 cut(s) 290, 432
HpaII CCGG 2 cut(s) 521, 743
HphI GGTGA 4 cut(s) 296, 580, 706, 832
Hpy166II GTNNAC 2 cut(s) 6, 372
Hpy188I TCNGA 3 cut(s) 140, 153, 950
Hpy188III TCNNGA 3 cut(s) 124, 634, 1212
Hpy8I GTNNAC 2 cut(s) 6, 372
Hpy99I CGWCG 3 cut(s) 175, 178, 226
HpyAV CCTTC 3 cut(s) 31, 206, 857
HpyCH4III ACNGT 2 cut(s) 507, 994
HpyCH4IV ACGT 2 cut(s) 173, 368
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 347, 353, 362, 977
HpyF3I CTNAG 3 cut(s) 86, 297, 357
HpySE526I ACGT 2 cut(s) 173, 368
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 7 cut(s) 75, 421, 763, 980, 1146, 1162, 1171
HspAI GCGC 1 cut(s) 812
Kzo9I GATC 4 cut(s) 414, 696, 876, 1252
LguI GCTCTTC 1 cut(s) 106
LmnI GCTCC 4 cut(s) 269, 680, 1085, 1119
Lsp1109I GCAGC 3 cut(s) 214, 319, 334
LweI GCATC 2 cut(s) 739, 849
MaeI CTAG 5 cut(s) 527, 561, 618, 627, 672
MaeII ACGT 2 cut(s) 173, 368
MaeIII GTNAC 2 cut(s) 796, 838
MalI GATC 4 cut(s) 416, 698, 878, 1254
MboI GATC 4 cut(s) 414, 696, 876, 1252
MboII GAAGA 7 cut(s) 3, 93, 139, 201, 1046, 1049, 1213
MfeI CAATTG 1 cut(s) 684
MhlI GDGCHC 3 cut(s) 610, 1199, 1207
MlsI TGGCCA 1 cut(s) 1163
MluCI AATT 6 cut(s) 537, 684, 726, 912, 927, 1014
MluNI TGGCCA 1 cut(s) 1163
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 976
Mox20I TGGCCA 1 cut(s) 1163
MroXI GAANNNNTTC 1 cut(s) 1054
MscI TGGCCA 1 cut(s) 1163
MseI TTAA 3 cut(s) 536, 725, 915
MslI CAYNNNNRTG 2 cut(s) 109, 1070
Msp20I TGGCCA 1 cut(s) 1163
MspA1I CMGCKG 2 cut(s) 205, 613
MspI CCGG 2 cut(s) 521, 743
MspR9I CCNGG 1 cut(s) 1088
MunI CAATTG 1 cut(s) 684
Mva1269I GAATGC 1 cut(s) 1077
MvaI CCWGG 1 cut(s) 1088
MwoI GCNNNNNNNGC 5 cut(s) 338, 347, 353, 362, 977
NcoI CCATGG 2 cut(s) 759, 1158
NdeII GATC 4 cut(s) 414, 696, 876, 1252
NlaIII CATG 7 cut(s) 75, 421, 763, 980, 1146, 1162, 1171
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 2 cut(s) 796, 838
NspI RCATGY 2 cut(s) 75, 1146
PceI AGGCCT 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 106
PcsI WCGNNNNNNNCGW 3 cut(s) 116, 176, 230
PctI GAATGC 1 cut(s) 1077
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 1 cut(s) 432
PflMI CCANNNNNTGG 1 cut(s) 1249
PkrI GCNGC 3 cut(s) 204, 334, 349
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PshBI ATTAAT 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 1086
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 1 cut(s) 1086
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 1 cut(s) 1171
PvuII CAGCTG 1 cut(s) 613
RsaI GTAC 1 cut(s) 883
RsaNI GTAC 1 cut(s) 882
RseI CAYNNNNRTG 2 cut(s) 109, 1070
SapI GCTCTTC 1 cut(s) 106
SaqAI TTAA 3 cut(s) 536, 725, 915
SatI GCNGC 3 cut(s) 203, 333, 348
Sau3AI GATC 4 cut(s) 414, 696, 876, 1252
Sau96I GGNCC 1 cut(s) 1171
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 1088
SduI GDGCHC 3 cut(s) 610, 1199, 1207
SfaNI GCATC 2 cut(s) 739, 849
SmiMI CAYNNNNRTG 2 cut(s) 109, 1070
SmlI CTYRAG 2 cut(s) 634, 655
SmoI CTYRAG 2 cut(s) 634, 655
Sse9I AATT 6 cut(s) 537, 684, 726, 912, 927, 1014
SseBI AGGCCT 1 cut(s) 720
SsiI CCGC 2 cut(s) 205, 309
SspI AATATT 2 cut(s) 567, 774
SspMI CTAG 5 cut(s) 527, 561, 618, 627, 672
StuI AGGCCT 1 cut(s) 720
StyD4I CCNGG 1 cut(s) 1086
StyI CCWWGG 4 cut(s) 384, 759, 1114, 1158
TaaI ACNGT 2 cut(s) 507, 994
TaiI ACGT 2 cut(s) 176, 371
TaqI TCGA 5 cut(s) 125, 170, 447, 850, 1211
TaqII GACCGA 2 cut(s) 433, 1197
TasI AATT 6 cut(s) 537, 684, 726, 912, 927, 1014
TatI WGTACW 1 cut(s) 881
TfiI GAWTC 1 cut(s) 432
Tru1I TTAA 3 cut(s) 536, 725, 915
Tru9I TTAA 3 cut(s) 536, 725, 915
TscAI CASTG 3 cut(s) 343, 861, 987
TseFI GTSAC 2 cut(s) 796, 838
TseI GCWGC 3 cut(s) 202, 332, 347
Tsp45I GTSAC 2 cut(s) 796, 838
TspDTI ATGAA 5 cut(s) 13, 93, 876, 965, 1047
TspRI CASTG 3 cut(s) 343, 861, 987
Van91I CCANNNNNTGG 1 cut(s) 1249
VspI ATTAAT 1 cut(s) 915
XapI RAATTY 2 cut(s) 927, 1014
XceI RCATGY 2 cut(s) 75, 1146
XcmI CCANNNNNNNNNTGG 1 cut(s) 1165
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 5 cut(s) 527, 561, 618, 627, 672
ZraI GACGTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.