RchiOBHm_Chr1g0359741

sodium metabolite cotransporter BASS3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008339
Physical Location & Seq
Reverse (-)
51602641 .. 51603877
1237 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58477

Sequence Viewer

Length: 597 bp
ATGTACAAGAATTGTACCTACAATTTTCGTGTGGTTCGTTTTCGAAGAGGGGGGGGGTTCAGAAATGGGGGTCTGTCGATAAAGGGTTGCTCGACGTCGCCGTTTATAGGAAGAGTTGGGTCACAGCGGAGGGATGGAAACTCTTCGTTGCTTTCGTTTGGGATAAACCCACCAACGGCTATGTCCAAAGGAGCCGAACAGAGTAGTGAGGACTCGTCTCAGGTCTTATCCGCAATGCTTCCATTTGTGGTTGCTGCCACTGCTATTGCTGCTTTGGCTCAGCCTGCCACGTTTACTTGGATATCCAAGGATTTATACGCCCCTGCTCTTGGTGGGATCATGCTCTCCATTGGAATCAAACTTTCCGTCGATGATTTTGCTCTTGCTGTCAAAAGACCTTTACCACTCTCTATTGGGTTTATCGCGCAGTATGTGCTGAAACCGGCTCTAGGGGTGTTAATTGCAATGGCATTTGGCGTGCCTAGAATATTCTACGCTGGGTTTGTCCTCACCACTTGTGTTGCAGGGGCACAGCTGTCTAGTTATGCTAGTTTCTTGAGCAAAGGAGATGTGGCCTTGAGTATTCCTCCTCACTAG

Protein Analysis

198

Amino Acids

20.86

Weight (kDa)

9.91

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 108 - 195 1.5e-17 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 98
AccII CGCG 1 cut(s) 425
AciI CCGC 2 cut(s) 127, 231
AclWI GGATC 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 2 cut(s) 5, 16
AfiI CCNNNNNNNGG 4 cut(s) 107, 175, 329, 449
AluBI AGCT 1 cut(s) 535
AluI AGCT 1 cut(s) 535
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 1 cut(s) 344
AoxI GGCC 1 cut(s) 573
ApeKI GCWGC 2 cut(s) 254, 269
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
Asp700I GAANNNNTTC 1 cut(s) 142
AspLEI GCGC 1 cut(s) 427
AsuHPI GGTGA 1 cut(s) 502
AsuII TTCGAA 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 532
BbvI GCAGC 2 cut(s) 241, 256
BccI CCATC 1 cut(s) 128
BceAI ACGGC 2 cut(s) 85, 192
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BcoDI GTCTC 1 cut(s) 222
BfaI CTAG 5 cut(s) 449, 483, 540, 549, 595
BisI GCNGC 2 cut(s) 255, 270
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 2 cut(s) 256, 271
BmiI GGNNCC 1 cut(s) 193
BplI GAGNNNNNCTC 1 cut(s) 571
Bpu1102I GCTNAGC 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 577
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 306
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 175, 329, 449
Bse118I RCCGGY 1 cut(s) 442
Bse3DI GCAATG 2 cut(s) 240, 471
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 139
BseLI CCNNNNNNNGG 4 cut(s) 107, 175, 329, 449
BseMI GCAATG 2 cut(s) 240, 471
BseMII CTCAG 2 cut(s) 233, 293
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 2 cut(s) 241, 256
BseYI CCCAGC 1 cut(s) 497
Bsh1236I CGCG 1 cut(s) 425
BshFI GGCC 1 cut(s) 575
BsiSI CCGG 1 cut(s) 443
BslI CCNNNNNNNGG 4 cut(s) 107, 175, 329, 449
BsmAI GTCTC 1 cut(s) 222
BsmBI CGTCTC 1 cut(s) 222
BsnI GGCC 1 cut(s) 575
Bsp119I TTCGAA 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 532
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 336
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 2 cut(s) 127, 231
BspANI GGCC 1 cut(s) 575
BspCNI CTCAG 2 cut(s) 232, 292
BspFNI CGCG 1 cut(s) 425
BspLI GGNNCC 1 cut(s) 193
BspPI GGATC 1 cut(s) 344
BspT104I TTCGAA 1 cut(s) 43
BsrDI GCAATG 2 cut(s) 240, 471
BsrFI RCCGGY 1 cut(s) 442
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 1 cut(s) 336
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 306
Bst6I CTCTTC 3 cut(s) 40, 106, 148
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 433
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 43
BstC8I GCNNGC 2 cut(s) 285, 479
BstDEI CTNAG 2 cut(s) 219, 279
BstF5I GGATG 1 cut(s) 139
BstFNI CGCG 1 cut(s) 425
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 1 cut(s) 339
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 1 cut(s) 336
BstMWI GCNNNNNNNGC 5 cut(s) 260, 269, 275, 284, 433
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 241, 256
BsuRI GGCC 1 cut(s) 575
BtsCI GGATG 1 cut(s) 139
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 285, 479
CfoI GCGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 442
Csp6I GTAC 2 cut(s) 4, 15
CspCI CAANNNNNGTGG 4 cut(s) 247, 282, 502, 537
CviAII CATG 1 cut(s) 340
CviJI RGCY 7 cut(s) 179, 194, 278, 283, 446, 535, 575
CviKI_1 RGCY 7 cut(s) 179, 194, 278, 283, 446, 535, 575
CviQI GTAC 2 cut(s) 4, 15
DdeI CTNAG 2 cut(s) 219, 279
DpnI GATC 1 cut(s) 338
DpnII GATC 1 cut(s) 336
Eam1104I CTCTTC 3 cut(s) 40, 106, 148
EarI CTCTTC 3 cut(s) 40, 106, 148
Eco130I CCWWGG 1 cut(s) 306
Eco32I GATATC 1 cut(s) 303
EcoRV GATATC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 1 cut(s) 343
FaiI YATR 6 cut(s) 107, 182, 316, 341, 432, 546
FatI CATG 1 cut(s) 339
Fnu4HI GCNGC 2 cut(s) 255, 270
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 2 cut(s) 255, 270
FspBI CTAG 5 cut(s) 449, 483, 540, 549, 595
GlaI GCGC 1 cut(s) 426
GluI GCNGC 2 cut(s) 255, 270
GsaI CCCAGC 1 cut(s) 501
HaeIII GGCC 1 cut(s) 575
HapII CCGG 1 cut(s) 443
HhaI GCGC 1 cut(s) 427
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 2 cut(s) 212, 354
HpaII CCGG 1 cut(s) 443
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 62
Hpy188III TCNNGA 1 cut(s) 556
Hpy8I GTNNAC 1 cut(s) 294
Hpy99I CGWCG 3 cut(s) 97, 100, 371
HpyCH4IV ACGT 2 cut(s) 95, 290
HpyCH4V TGCA 2 cut(s) 464, 524
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 269, 275, 284, 433
HpyF3I CTNAG 2 cut(s) 219, 279
HpySE526I ACGT 2 cut(s) 95, 290
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 1 cut(s) 425
Kzo9I GATC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 6 cut(s) 206, 297, 336, 456, 483, 510
Lsp1109I GCAGC 2 cut(s) 241, 256
MaeI CTAG 5 cut(s) 449, 483, 540, 549, 595
MaeII ACGT 2 cut(s) 95, 290
MaeIII GTNAC 1 cut(s) 120
MalI GATC 1 cut(s) 338
MboI GATC 1 cut(s) 336
MboII GAAGA 3 cut(s) 57, 123, 135
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 3 cut(s) 10, 22, 459
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 5 cut(s) 41, 123, 202, 518, 597
MroXI GAANNNNTTC 1 cut(s) 142
MseI TTAA 1 cut(s) 458
MspA1I CMGCKG 2 cut(s) 127, 535
MspI CCGG 1 cut(s) 443
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 5 cut(s) 260, 269, 275, 284, 433
NdeII GATC 1 cut(s) 336
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 120
NspV TTCGAA 1 cut(s) 43
PcsI WCGNNNNNNNCGW 3 cut(s) 34, 98, 152
PdmI GAANNNNTTC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 354
PkrI GCNGC 2 cut(s) 256, 271
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 2 cut(s) 5, 16
RsaNI GTAC 2 cut(s) 4, 15
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 2 cut(s) 255, 270
Sau3AI GATC 1 cut(s) 336
SchI GAGTC 1 cut(s) 206
SduI GDGCHC 1 cut(s) 532
SetI ASST 6 cut(s) 20, 98, 225, 293, 400, 537
SfuI TTCGAA 1 cut(s) 43
SmlI CTYRAG 2 cut(s) 556, 577
SmoI CTYRAG 2 cut(s) 556, 577
Sse9I AATT 3 cut(s) 10, 22, 459
SsiI CCGC 2 cut(s) 127, 231
SspI AATATT 1 cut(s) 489
SspMI CTAG 5 cut(s) 449, 483, 540, 549, 595
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 2 cut(s) 98, 293
TaqI TCGA 4 cut(s) 43, 77, 92, 369
TasI AATT 3 cut(s) 10, 22, 459
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 1 cut(s) 354
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 120
TseI GCWGC 2 cut(s) 254, 269
Tsp45I GTSAC 1 cut(s) 120
TspGWI ACGGA 1 cut(s) 355
TspRI CASTG 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 142
XspI CTAG 5 cut(s) 449, 483, 540, 549, 595
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.