AT3G25440

CRM domain-containing protein At3g25440

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007087
Physical Location & Seq
Forward (+)
9223956 .. 9225774
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25440.5

Sequence Viewer

Length: 1335 bp
ATGGGGTTTCTGACGGCGGCGATTAGGGTTTCGACATCAGCAGCCTCAATGCTTCTACGCCGTAAATCTCGGCAGCTAGGGTTCCTAGCCTCCTCCTCGCCGCTTTTTTCCAGCTTTAATTCCATGAATCGCACTATCAGCTCGTGTAATATTGTGTGCAAGGTTTTGCATAAGGAATCTCTTACTAGACCAATGTGGAACGTTAGCTTCTTAAGATCCTCTTCGTTTCATTCAACTCCAGCTCGTGAGACCGGTGATGATGACATTAGCAAATCTGAGAACAGTTCGAGTCAAGATGGAGACAGTTGTACTAAATTAAAGAGGAAGAAGCTGAAAGGTAAGCGTGCGGTTGTGCGGTGGCTAAAGTTTTTCCGGTGGAAGAAAAAGAAAGAATTTGAAAGAATGACTTCTGAAGAAAAGATACTTAACAAACTAAGAAAGGCTCGAAAGAAAGAGGAACGGCTTATGGAGACGATGAAGAAGCTGGAACCTTCAGAGTCAGCAGAAACGACACATGACCCTGAGATATTGACTCCAGAAGAACATTTTTATTATCTGAAAATGGGTTTGAAGTGCAAGAATTATGTTCCTGTAGGGAGACGTGGGATTTACCAAGGAGTGATTCTGAACATGCATCTTCACTGGAAGAAGCATCAGACGCTACAGGTTGTGATCAAAACGTTTACACCTGATGAAGTGAAGGAGATTGCTGTTGAGTTAGCGAGGTTGACTGGAGGGATTGTGCTTGATGTACATGAAGGTAACACGATAATTATGTACAGAGGGAAGAACTATGTGCAACCTCCTACTGAAATCATGTCGCCGAGGATTACTCTTCCGAGGAAAAAGGCTTTGGATAAGTCTAAATGTAGGGATGCGCTTCGTGCGGTTAGGAAGTATATTCCGAGGCTTGAACAGGAGCTCCAGTTGCTTCAAGCACAAGCTGAAACGAAGCGGGATTACACGAATGTGAAGGTTGATGATAATCAAGAGAGGTCAGAAGAGTTGAAGAAGATTATAGAAAGAAGCGAAGAGTGTTTGGAGGATGAGCAAGAAGAAGATGAAGCAGGATTAGAACTAGCGACGGATTCAGATCTTTCGGATATTTTTGAGACTGATTCGGAATTAGAAGATGCGAAAACAGAGCGACCACTTTTTCTTGAGGAGTTTGAGAAATTCCCAGCTATAAACAACAGAGAAGACGAAGACTTTGGGGATCTCGGGAAGGCTAAATCAGAAGGTGAGGAGAATGATGATGATAAGTCACCAAACTTTGATGAAGTAGATAAGATGTTTCTTCGGGCTGCTTTTCTTTTAAAGAAGAAAAGACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CRS1_YhbY EF-hand_1 EF-hand_5

Protein Analysis

444

Amino Acids

51.47

Weight (kDa)

8.62

Isoelectric Point (pI)

62.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 22 - 106 1e-15 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 17, 101, 347, 355, 887, 955
AclI AACGTT 2 cut(s) 201, 680
AclWI GGATC 2 cut(s) 210, 1224
AcsI RAATTY 2 cut(s) 392, 1175
AcuI CTGAAG 2 cut(s) 432, 477
AfaI GTAC 3 cut(s) 310, 753, 779
AflII CTTAAG 1 cut(s) 211
AgeI ACCGGT 1 cut(s) 251
AgsI TTSAA 6 cut(s) 234, 398, 571, 914, 935, 1009
AjiI CACGTC 1 cut(s) 602
AjuI GAANNNNNNNTTGG 4 cut(s) 606, 638, 836, 868
AleI CACNNNNGTG 1 cut(s) 968
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 5 cut(s) 242, 294, 464, 592, 1106
AlwI GGATC 2 cut(s) 210, 1224
Ama87I CYCGRG 1 cut(s) 1220
ApeKI GCWGC 3 cut(s) 41, 73, 1304
ApoI RAATTY 2 cut(s) 392, 1175
ArsI GACNNNNNNTTYG 2 cut(s) 1193, 1225
AsiGI ACCGGT 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 1 cut(s) 880
AsuHPI GGTGA 3 cut(s) 266, 1253, 1257
AvaI CYCGRG 1 cut(s) 1220
BanII GRGCYC 1 cut(s) 924
BarI GAAGNNNNNNTAC 4 cut(s) 405, 437, 944, 976
BauI CACGAG 2 cut(s) 142, 243
BbsI GAAGAC 2 cut(s) 1206, 1212
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 3 cut(s) 53, 85, 1291
BccI CCATC 1 cut(s) 290
BceAI ACGGC 3 cut(s) 30, 45, 476
BclI TGATCA 1 cut(s) 672
BcoDI GTCTC 5 cut(s) 242, 294, 464, 592, 1106
BfaI CTAG 4 cut(s) 77, 86, 186, 1079
BfmI CTRYAG 2 cut(s) 591, 662
BfrI CTTAAG 1 cut(s) 211
BglII AGATCT 1 cut(s) 1093
BisI GCNGC 5 cut(s) 18, 42, 74, 101, 1305
BlsI GCNGC 5 cut(s) 19, 43, 75, 102, 1306
BmeT110I CYCGRG 1 cut(s) 1220
BmgBI CACGTC 1 cut(s) 602
BmiI GGNNCC 2 cut(s) 83, 489
BmsI GCATC 4 cut(s) 643, 661, 865, 1123
BpiI GAAGAC 2 cut(s) 1206, 1212
BplI GAGNNNNNCTC 2 cut(s) 817, 849
BpmI CTGGAG 4 cut(s) 222, 519, 753, 908
BpuEI CTTGAG 1 cut(s) 1181
BsaBI GATNNNNATC 2 cut(s) 984, 1092
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 4 cut(s) 613, 824, 839, 905
BsaWI WCCGGW 2 cut(s) 251, 372
BsaXI ACNNNNNCTCC 6 cut(s) 726, 756, 906, 911, 936, 941
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 3 cut(s) 647, 736, 925
Bse8I GATNNNNATC 2 cut(s) 984, 1092
BseDI CCNNGG 4 cut(s) 613, 824, 839, 905
BseGI GGATG 2 cut(s) 880, 1051
BseJI GATNNNNATC 2 cut(s) 984, 1092
BseMII CTCAG 2 cut(s) 267, 513
BseNI ACTGG 3 cut(s) 647, 736, 925
BseRI GAGGAG 4 cut(s) 82, 85, 1178, 1259
BseXI GCAGC 3 cut(s) 53, 85, 1291
BseYI CCCAGC 1 cut(s) 1180
BshTI ACCGGT 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 924
BsiHKCI CYCGRG 1 cut(s) 1220
BsiSI CCGG 2 cut(s) 252, 373
BsmAI GTCTC 5 cut(s) 242, 294, 464, 592, 1106
BsmBI CGTCTC 2 cut(s) 464, 592
Bso31I GGTCTC 1 cut(s) 242
BsoBI CYCGRG 1 cut(s) 1220
Bsp1286I GDGCHC 1 cut(s) 924
Bsp1407I TGTACA 2 cut(s) 751, 777
Bsp143I GATC 4 cut(s) 215, 672, 1093, 1216
BspACI CCGC 6 cut(s) 17, 101, 347, 355, 887, 955
BspCNI CTCAG 2 cut(s) 268, 514
BspLI GGNNCC 2 cut(s) 83, 489
BspPI GGATC 2 cut(s) 210, 1224
BspTI CTTAAG 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 251
BsrGI TGTACA 2 cut(s) 751, 777
BsrI ACTGG 3 cut(s) 647, 736, 925
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 4 cut(s) 613, 824, 839, 905
BssMI GATC 4 cut(s) 215, 672, 1093, 1216
BssSI CACGAG 2 cut(s) 142, 243
BssT1I CCWWGG 1 cut(s) 613
Bst2BI CACGAG 2 cut(s) 142, 243
Bst4CI ACNGT 2 cut(s) 284, 305
Bst6I CTCTTC 4 cut(s) 226, 840, 996, 1026
BstAFI CTTAAG 1 cut(s) 211
BstAUI TGTACA 2 cut(s) 751, 777
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 3 cut(s) 276, 434, 522
BstF5I GGATG 2 cut(s) 880, 1051
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 4 cut(s) 218, 675, 1096, 1219
BstMAI GTCTC 5 cut(s) 242, 294, 464, 592, 1106
BstMBI GATC 4 cut(s) 215, 672, 1093, 1216
BstMWI GCNNNNNNNGC 4 cut(s) 138, 658, 884, 928
BstNSI RCATGY 1 cut(s) 634
BstSFI CTRYAG 2 cut(s) 591, 662
BstV1I GCAGC 3 cut(s) 53, 85, 1291
BstV2I GAAGAC 2 cut(s) 1206, 1212
BstX2I RGATCY 3 cut(s) 215, 1093, 1216
BstYI RGATCY 3 cut(s) 215, 1093, 1216
BtrI CACGTC 1 cut(s) 602
BtsCI GGATG 2 cut(s) 880, 1051
BtsIMutI CAGTG 1 cut(s) 640
Cac8I GCNNGC 1 cut(s) 345
CfoI GCGC 1 cut(s) 880
Cfr10I RCCGGY 1 cut(s) 251
CseI GACGC 1 cut(s) 667
Csp6I GTAC 3 cut(s) 309, 752, 778
CspAI ACCGGT 1 cut(s) 251
CviAII CATG 5 cut(s) 124, 515, 631, 755, 817
CviQI GTAC 3 cut(s) 309, 752, 778
DdeI CTNAG 3 cut(s) 276, 434, 522
DpnI GATC 4 cut(s) 217, 674, 1095, 1218
DpnII GATC 4 cut(s) 215, 672, 1093, 1216
DraI TTTAAA 1 cut(s) 1317
Eam1104I CTCTTC 4 cut(s) 226, 840, 996, 1026
EarI CTCTTC 4 cut(s) 226, 840, 996, 1026
Ecl136II GAGCTC 1 cut(s) 922
Eco130I CCWWGG 1 cut(s) 613
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 2 cut(s) 432, 477
Eco88I CYCGRG 1 cut(s) 1220
EcoICRI GAGCTC 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 613
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 1 cut(s) 613
Esp3I CGTCTC 2 cut(s) 464, 592
FaeI CATG 5 cut(s) 127, 518, 634, 758, 820
FalI AAGNNNNNCTT 4 cut(s) 205, 237, 391, 423
FatI CATG 5 cut(s) 123, 514, 630, 754, 816
FauI CCCGC 1 cut(s) 948
FbaI TGATCA 1 cut(s) 672
Fnu4HI GCNGC 5 cut(s) 18, 42, 74, 101, 1305
FokI GGATG 2 cut(s) 887, 1058
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 5 cut(s) 18, 42, 74, 101, 1305
FspBI CTAG 4 cut(s) 77, 86, 186, 1079
GlaI GCGC 1 cut(s) 879
GluI GCNGC 5 cut(s) 18, 42, 74, 101, 1305
GsaI CCCAGC 1 cut(s) 1184
GsuI CTGGAG 4 cut(s) 222, 519, 753, 908
HapII CCGG 2 cut(s) 252, 373
HgaI GACGC 1 cut(s) 667
HhaI GCGC 1 cut(s) 880
Hin1II CATG 5 cut(s) 127, 518, 634, 758, 820
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HinfI GANTC 8 cut(s) 127, 176, 289, 497, 532, 622, 1088, 1118
HpaII CCGG 2 cut(s) 252, 373
HphI GGTGA 3 cut(s) 266, 1253, 1257
Hpy166II GTNNAC 2 cut(s) 684, 729
Hpy188III TCNNGA 6 cut(s) 245, 293, 536, 989, 1160, 1222
Hpy8I GTNNAC 2 cut(s) 684, 729
Hpy99I CGWCG 1 cut(s) 1087
HpyAV CCTTC 6 cut(s) 501, 694, 752, 967, 1219, 1232
HpyCH4III ACNGT 2 cut(s) 284, 305
HpyCH4IV ACGT 3 cut(s) 201, 601, 680
HpyCH4V TGCA 5 cut(s) 159, 169, 576, 634, 799
HpyF10VI GCNNNNNNNGC 4 cut(s) 138, 658, 884, 928
HpyF3I CTNAG 3 cut(s) 276, 434, 522
HpySE526I ACGT 3 cut(s) 201, 601, 680
Hsp92II CATG 5 cut(s) 127, 518, 634, 758, 820
HspAI GCGC 1 cut(s) 878
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 4 cut(s) 215, 672, 1093, 1216
LmnI GCTCC 2 cut(s) 919, 927
Lsp1109I GCAGC 3 cut(s) 53, 85, 1291
LweI GCATC 4 cut(s) 643, 661, 865, 1123
MaeI CTAG 4 cut(s) 77, 86, 186, 1079
MaeII ACGT 3 cut(s) 201, 601, 680
MaeIII GTNAC 2 cut(s) 761, 1263
MalI GATC 4 cut(s) 217, 674, 1095, 1218
MboI GATC 4 cut(s) 215, 672, 1093, 1216
MflI RGATCY 3 cut(s) 215, 1093, 1216
MhlI GDGCHC 1 cut(s) 924
MluCI AATT 7 cut(s) 118, 314, 392, 580, 771, 1124, 1175
MlyI GAGTC 3 cut(s) 298, 506, 526
Mph1103I ATGCAT 1 cut(s) 636
MroXI GAANNNNTTC 1 cut(s) 406
MseI TTAA 5 cut(s) 117, 212, 317, 426, 1316
MslI CAYNNNNRTG 1 cut(s) 968
MspCI CTTAAG 1 cut(s) 211
MspI CCGG 2 cut(s) 252, 373
MwoI GCNNNNNNNGC 4 cut(s) 138, 658, 884, 928
NdeII GATC 4 cut(s) 215, 672, 1093, 1216
NlaIII CATG 5 cut(s) 127, 518, 634, 758, 820
NlaIV GGNNCC 2 cut(s) 83, 489
NmeAIII GCCGAG 2 cut(s) 49, 849
NmuCI GTSAC 1 cut(s) 1263
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 634
OliI CACNNNNGTG 1 cut(s) 968
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 5 cut(s) 127, 176, 622, 1088, 1118
PinAI ACCGGT 1 cut(s) 251
PkrI GCNGC 5 cut(s) 19, 43, 75, 102, 1306
PleI GAGTC 3 cut(s) 297, 505, 526
PpsI GAGTC 3 cut(s) 297, 505, 526
Psp124BI GAGCTC 1 cut(s) 924
Psp1406I AACGTT 2 cut(s) 201, 680
PspFI CCCAGC 1 cut(s) 1180
PspN4I GGNNCC 2 cut(s) 83, 489
PsuI RGATCY 3 cut(s) 215, 1093, 1216
RsaI GTAC 3 cut(s) 310, 753, 779
RsaNI GTAC 3 cut(s) 309, 752, 778
RseI CAYNNNNRTG 1 cut(s) 968
SacI GAGCTC 1 cut(s) 924
SaqAI TTAA 5 cut(s) 117, 212, 317, 426, 1316
SatI GCNGC 5 cut(s) 18, 42, 74, 101, 1305
Sau3AI GATC 4 cut(s) 215, 672, 1093, 1216
SchI GAGTC 3 cut(s) 298, 506, 526
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 4 cut(s) 643, 661, 865, 1123
SfcI CTRYAG 2 cut(s) 591, 662
SmiMI CAYNNNNRTG 1 cut(s) 968
SmlI CTYRAG 2 cut(s) 211, 1160
SmoI CTYRAG 2 cut(s) 211, 1160
Sse9I AATT 7 cut(s) 118, 314, 392, 580, 771, 1124, 1175
SsiI CCGC 6 cut(s) 17, 101, 347, 355, 887, 955
SspI AATATT 1 cut(s) 151
SspMI CTAG 4 cut(s) 77, 86, 186, 1079
SstI GAGCTC 1 cut(s) 924
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 2 cut(s) 284, 305
TaiI ACGT 3 cut(s) 204, 604, 683
TaqI TCGA 3 cut(s) 32, 287, 445
TasI AATT 7 cut(s) 118, 314, 392, 580, 771, 1124, 1175
TatI WGTACW 3 cut(s) 308, 751, 777
TauI GCSGC 2 cut(s) 20, 103
TfiI GAWTC 5 cut(s) 127, 176, 622, 1088, 1118
Tru1I TTAA 5 cut(s) 117, 212, 317, 426, 1316
Tru9I TTAA 5 cut(s) 117, 212, 317, 426, 1316
TscAI CASTG 1 cut(s) 647
TseFI GTSAC 1 cut(s) 1263
TseI GCWGC 3 cut(s) 41, 73, 1304
Tsp45I GTSAC 1 cut(s) 1263
TspDTI ATGAA 7 cut(s) 140, 218, 491, 708, 771, 1077, 1293
TspGWI ACGGA 1 cut(s) 1100
TspRI CASTG 1 cut(s) 647
Vha464I CTTAAG 1 cut(s) 211
XapI RAATTY 2 cut(s) 392, 1175
XceI RCATGY 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 406
XspI CTAG 4 cut(s) 77, 86, 186, 1079
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.