AT3G26115

D-cysteine desulfhydrase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002795
Physical Location & Seq
Forward (+)
9542268 .. 9544497
2230 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G26115.3

Sequence Viewer

Length: 1284 bp
ATGAAGGTCCAACGGTCAACTTTTTTGGCGGTGACCGGAAAATCAAATCTTCATCACTTTCACTCGTCTTCTCAGAGAATTCCCATTACTTCCGAGATCGATTCACGAGAGTTTGTGTCTAAATTACTCGATAGAAAATGGGGATTACAGTGTCCTGCATCTCCAATTCAGCAGATTAGTGTTTCTTCTGTCAAAGGAATCGATAAATTCTCCTTCTTGAACAACACTCGTCCACATCTCGGAGATGAAATGTCGAAGTCGAAGCAAGGATCTTCTTTTTACATTCTTAGAGATGATCTTTTACATCCTCTTGTGAATGGTAACAAAGCTAGAAAGCTTGATGCTTTGTTACCACTTGTTGAAGATCATAAAGTGACTGATTTGGTTACATGTGGAGGTTGTCAAAGTGCACATACAGCTGCTGTTGCTGTTTCTTGTGCTGAGAGAGGTCTAAGATCACATTTGCTACTGCGTGGAGAACAGCCTGATGTTTTGACTGGTTATAACCTTGTTTCAACTATGTATGGAAATGTTCAGTATGTTCCAAGATCGAGGTATGCGAATAGAGAGGAAATGCTAAGAACTTATGCGGATCTTGTTGCTGGGGAAGATGGTACTGTCTTATGGGCTAAAGACATTGTTGAGGGACGTGACACTATGAATGTTGCAAAAATGGATGATTTTTCGAGTATGAAAACTTCTCGTAGAAAGGTTTTGATCGTTAATGAAGGCGCAGGAGATGCTCTTGCATTACTTGGTATGTTTCGGTTAGTGCAGCATTTGTCACAGGATCATTTACTCGGGAAGAAGAGGCCGGTTAAATTTGTAGTGGATGCTGGTACTGGAACAACTGCTGTGGGTTTAGGAGTTGCAGCTATGTCTTTAGGGCTTCCATGGGAAATCAATGCTGTGATGCTAGCTGATACACTCAAAAATTACAAGAGACATGAAGATCACTTGATAGCAGAATTTTCGAGGCAGTTTCCTGGCTCAGTCTTCTGCAGCGGCTTGGATATGAATCAAATGATCAAATGGATAGATCGTCAACACCCGAGAAAATTTGGGAAGGTCTTGGAAGGAGAAGTGGAGATGTGTCGGAAAATTGCACAGGAGACAGGAGTTTTAGTGGATCCAATGTATACTTTAGCCGCTTGGGAGACAGCAACAGAGCTTGTGCAGGACGAGAAATCAAGCATTGTAGTGATGCTTCACACTGGAGGCACTCTAGGGATGTTTGGTCTTGCTCAAAGATACAAAACTTGCTTCACTAACTTGAAAGATTAG

Protein Analysis

427

Amino Acids

47.43

Weight (kDa)

8.7

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 8 - 323 5.2e-11 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AccI GTMKAC 1 cut(s) 1139
AciI CCGC 4 cut(s) 29, 590, 1005, 1149
AclWI GGATC 5 cut(s) 277, 600, 798, 1124, 1137
AcsI RAATTY 5 cut(s) 78, 206, 821, 968, 1058
AfaI GTAC 2 cut(s) 616, 841
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 389
AgsI TTSAA 4 cut(s) 220, 362, 516, 1276
AjiI CACGTC 1 cut(s) 650
AjnI CCWGG 1 cut(s) 985
AluBI AGCT 6 cut(s) 329, 337, 419, 875, 920, 1171
AluI AGCT 6 cut(s) 329, 337, 419, 875, 920, 1171
Alw21I GWGCWC 1 cut(s) 412
Alw26I GTCTC 3 cut(s) 937, 1106, 1151
Alw44I GTGCAC 1 cut(s) 408
AlwI GGATC 5 cut(s) 277, 600, 798, 1124, 1137
AlwNI CAGNNNCTG 1 cut(s) 422
Ama87I CYCGRG 2 cut(s) 800, 1051
AoxI GGCC 1 cut(s) 812
ApaLI GTGCAC 1 cut(s) 408
ApeKI GCWGC 4 cut(s) 419, 775, 872, 1002
ApoI RAATTY 5 cut(s) 78, 206, 821, 968, 1058
AspLEI GCGC 1 cut(s) 734
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 43
AsuNHI GCTAGC 1 cut(s) 916
AvaI CYCGRG 2 cut(s) 800, 1051
AvaII GGWCC 1 cut(s) 7
BaeGI GKGCMC 1 cut(s) 412
BamHI GGATCC 1 cut(s) 1129
BarI GAAGNNNNNNTAC 4 cut(s) 682, 714, 1191, 1223
BauI CACGAG 1 cut(s) 105
BbsI GAAGAC 2 cut(s) 60, 988
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 4 cut(s) 406, 787, 884, 1014
BccI CCATC 1 cut(s) 605
BcgI CGANNNNNNTGC 2 cut(s) 954, 988
BciT130I CCWGG 1 cut(s) 987
BclI TGATCA 1 cut(s) 1026
BcoDI GTCTC 3 cut(s) 937, 1106, 1151
BfaI CTAG 3 cut(s) 330, 917, 1226
BfmI CTRYAG 1 cut(s) 1000
BisI GCNGC 6 cut(s) 420, 776, 873, 1003, 1006, 1149
BlsI GCNGC 6 cut(s) 421, 777, 874, 1004, 1007, 1150
Bme1390I CCNGG 1 cut(s) 987
Bme18I GGWCC 1 cut(s) 7
BmeT110I CYCGRG 2 cut(s) 800, 1051
BmgBI CACGTC 1 cut(s) 650
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 1131
BmrFI CCNGG 1 cut(s) 987
BmsI GCATC 6 cut(s) 167, 331, 730, 823, 903, 1194
BmtI GCTAGC 1 cut(s) 920
BpiI GAAGAC 2 cut(s) 60, 988
BpmI CTGGAG 1 cut(s) 1236
Bsa29I ATCGAT 2 cut(s) 99, 201
BsaBI GATNNNNATC 1 cut(s) 1017
BsaJI CCNNGG 1 cut(s) 893
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 1110, 1140
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 814
Bse1I ACTGG 3 cut(s) 502, 847, 1219
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 1 cut(s) 987
BseCI ATCGAT 2 cut(s) 99, 201
BseDI CCNNGG 1 cut(s) 893
BseGI GGATG 4 cut(s) 304, 682, 838, 1236
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 3 cut(s) 86, 432, 1005
BseNI ACTGG 3 cut(s) 502, 847, 1219
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 4 cut(s) 406, 787, 884, 1014
BseYI CCCAGC 1 cut(s) 602
BsgI GTGCAG 2 cut(s) 794, 1196
BshFI GGCC 1 cut(s) 814
BshVI ATCGAT 2 cut(s) 99, 201
BsiHKAI GWGCWC 1 cut(s) 412
BsiHKCI CYCGRG 2 cut(s) 800, 1051
BsiSI CCGG 2 cut(s) 36, 815
BslFI GGGAC 1 cut(s) 660
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 3 cut(s) 937, 1106, 1151
BsmFI GGGAC 1 cut(s) 660
BsnI GGCC 1 cut(s) 814
BsoBI CYCGRG 2 cut(s) 800, 1051
Bsp1286I GDGCHC 1 cut(s) 412
Bsp19I CCATGG 1 cut(s) 893
BspACI CCGC 4 cut(s) 29, 590, 1005, 1149
BspANI GGCC 1 cut(s) 814
BspCNI CTCAG 3 cut(s) 85, 433, 1004
BspDI ATCGAT 2 cut(s) 99, 201
BspLI GGNNCC 1 cut(s) 1131
BspMAI CTGCAG 1 cut(s) 1004
BspOI GCTAGC 1 cut(s) 920
BspPI GGATC 5 cut(s) 277, 600, 798, 1124, 1137
BsrFI RCCGGY 1 cut(s) 814
BsrI ACTGG 3 cut(s) 502, 847, 1219
BssAI RCCGGY 1 cut(s) 814
BssECI CCNNGG 1 cut(s) 893
BssNAI GTATAC 1 cut(s) 1140
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 893
Bst1107I GTATAC 1 cut(s) 1140
Bst2BI CACGAG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 987
Bst4CI ACNGT 3 cut(s) 15, 150, 619
Bst6I CTCTTC 1 cut(s) 803
BstAPI GCANNNNNTGC 1 cut(s) 740
BstC8I GCNNGC 1 cut(s) 918
BstDEI CTNAG 6 cut(s) 72, 287, 441, 452, 578, 991
BstDSI CCRYGG 1 cut(s) 893
BstEII GGTNACC 1 cut(s) 31
BstF5I GGATG 4 cut(s) 304, 682, 838, 1236
BstHHI GCGC 1 cut(s) 734
BstMAI GTCTC 3 cut(s) 937, 1106, 1151
BstMWI GCNNNNNNNGC 3 cut(s) 416, 425, 740
BstNI CCWGG 1 cut(s) 987
BstNSI RCATGY 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 985
BstSFI CTRYAG 1 cut(s) 1000
BstSLI GKGCMC 1 cut(s) 412
BstV1I GCAGC 4 cut(s) 406, 787, 884, 1014
BstV2I GAAGAC 2 cut(s) 60, 988
BstX2I RGATCY 3 cut(s) 269, 592, 1129
BstYI RGATCY 3 cut(s) 269, 592, 1129
BstZ17I GTATAC 1 cut(s) 1140
Bsu15I ATCGAT 2 cut(s) 99, 201
BsuRI GGCC 1 cut(s) 814
BsuTUI ATCGAT 2 cut(s) 99, 201
BtgI CCRYGG 1 cut(s) 893
BtrI CACGTC 1 cut(s) 650
BtsCI GGATG 4 cut(s) 304, 682, 838, 1236
BtsIMutI CAGTG 2 cut(s) 155, 1212
Cac8I GCNNGC 1 cut(s) 918
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 734
Cfr10I RCCGGY 1 cut(s) 814
Cfr13I GGNCC 1 cut(s) 7
ClaI ATCGAT 2 cut(s) 99, 201
Csp6I GTAC 2 cut(s) 615, 840
CspCI CAANNNNNGTGG 2 cut(s) 837, 872
CviAII CATG 3 cut(s) 390, 894, 947
CviQI GTAC 2 cut(s) 615, 840
DdeI CTNAG 6 cut(s) 72, 287, 441, 452, 578, 991
Eam1104I CTCTTC 1 cut(s) 803
EarI CTCTTC 1 cut(s) 803
Eco130I CCWWGG 1 cut(s) 893
Eco47I GGWCC 1 cut(s) 7
Eco88I CYCGRG 2 cut(s) 800, 1051
Eco91I GGTNACC 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 985
EcoT14I CCWWGG 1 cut(s) 893
ErhI CCWWGG 1 cut(s) 893
FaeI CATG 3 cut(s) 393, 897, 950
FaqI GGGAC 1 cut(s) 660
FatI CATG 3 cut(s) 389, 893, 946
FbaI TGATCA 1 cut(s) 1026
FblI GTMKAC 1 cut(s) 1139
Fnu4HI GCNGC 6 cut(s) 420, 776, 873, 1003, 1006, 1149
FokI GGATG 4 cut(s) 291, 689, 845, 1243
Fsp4HI GCNGC 6 cut(s) 420, 776, 873, 1003, 1006, 1149
FspBI CTAG 3 cut(s) 330, 917, 1226
GlaI GCGC 1 cut(s) 733
GluI GCNGC 6 cut(s) 420, 776, 873, 1003, 1006, 1149
GsaI CCCAGC 1 cut(s) 606
GsuI CTGGAG 1 cut(s) 1236
HaeIII GGCC 1 cut(s) 814
HapII CCGG 2 cut(s) 36, 815
HhaI GCGC 1 cut(s) 734
Hin1II CATG 3 cut(s) 393, 897, 950
Hin6I GCGC 1 cut(s) 732
HinP1I GCGC 1 cut(s) 732
HincII GTYRAC 2 cut(s) 18, 1046
HindII GTYRAC 2 cut(s) 18, 1046
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 3 cut(s) 101, 198, 1018
HpaII CCGG 2 cut(s) 36, 815
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 5 cut(s) 18, 233, 410, 1046, 1140
Hpy188I TCNGA 4 cut(s) 75, 94, 242, 1098
Hpy188III TCNNGA 3 cut(s) 105, 217, 802
Hpy8I GTNNAC 5 cut(s) 18, 233, 410, 1046, 1140
HpyAV CCTTC 4 cut(s) 223, 722, 1060, 1070
HpyCH4III ACNGT 3 cut(s) 15, 150, 619
HpyCH4IV ACGT 1 cut(s) 649
HpyCH4V TGCA 9 cut(s) 158, 410, 668, 749, 775, 872, 1002, 1106, 1177
HpyF10VI GCNNNNNNNGC 3 cut(s) 416, 425, 740
HpyF3I CTNAG 6 cut(s) 72, 287, 441, 452, 578, 991
HpySE526I ACGT 1 cut(s) 649
Hsp92II CATG 3 cut(s) 393, 897, 950
HspAI GCGC 1 cut(s) 732
Ksp22I TGATCA 1 cut(s) 1026
Lsp1109I GCAGC 4 cut(s) 406, 787, 884, 1014
LweI GCATC 6 cut(s) 167, 331, 730, 823, 903, 1194
MaeI CTAG 3 cut(s) 330, 917, 1226
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 7 cut(s) 31, 320, 348, 373, 385, 650, 783
MflI RGATCY 3 cut(s) 269, 592, 1129
MhlI GDGCHC 1 cut(s) 412
MluCI AATT 9 cut(s) 78, 122, 165, 206, 821, 934, 968, 1058, 1101
MmeI TCCRAC 2 cut(s) 34, 1076
MnlI CCTC 9 cut(s) 318, 389, 440, 546, 562, 637, 804, 969, 1211
MseI TTAA 2 cut(s) 723, 819
MslI CAYNNNNRTG 1 cut(s) 1199
MspA1I CMGCKG 2 cut(s) 419, 1005
MspI CCGG 2 cut(s) 36, 815
MspR9I CCNGG 1 cut(s) 987
MvaI CCWGG 1 cut(s) 987
MwoI GCNNNNNNNGC 3 cut(s) 416, 425, 740
NcoI CCATGG 1 cut(s) 893
NheI GCTAGC 1 cut(s) 916
NlaIII CATG 3 cut(s) 393, 897, 950
NlaIV GGNNCC 1 cut(s) 1131
NmuCI GTSAC 4 cut(s) 31, 373, 650, 783
NspI RCATGY 1 cut(s) 393
PciI ACATGT 1 cut(s) 389
PcsI WCGNNNNNNNCGW 1 cut(s) 557
PfeI GAWTC 3 cut(s) 101, 198, 1018
PkrI GCNGC 6 cut(s) 421, 777, 874, 1004, 1007, 1150
PscI ACATGT 1 cut(s) 389
PsiI TTATAA 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 985
PspEI GGTNACC 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 1 cut(s) 985
PspN4I GGNNCC 1 cut(s) 1131
PspPI GGNCC 1 cut(s) 7
PstI CTGCAG 1 cut(s) 1004
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 3 cut(s) 269, 592, 1129
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 616, 841
RsaNI GTAC 2 cut(s) 615, 840
RseI CAYNNNNRTG 1 cut(s) 1199
SaqAI TTAA 2 cut(s) 723, 819
SatI GCNGC 6 cut(s) 420, 776, 873, 1003, 1006, 1149
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 1 cut(s) 987
SduI GDGCHC 1 cut(s) 412
SfaNI GCATC 6 cut(s) 167, 331, 730, 823, 903, 1194
SfcI CTRYAG 1 cut(s) 1000
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 1199
Sse9I AATT 9 cut(s) 78, 122, 165, 206, 821, 934, 968, 1058, 1101
SsiI CCGC 4 cut(s) 29, 590, 1005, 1149
SspMI CTAG 3 cut(s) 330, 917, 1226
StyD4I CCNGG 1 cut(s) 985
StyI CCWWGG 1 cut(s) 893
TaaI ACNGT 3 cut(s) 15, 150, 619
TaiI ACGT 1 cut(s) 652
TaqI TCGA 8 cut(s) 99, 129, 201, 254, 260, 551, 686, 974
TasI AATT 9 cut(s) 78, 122, 165, 206, 821, 934, 968, 1058, 1101
TauI GCSGC 2 cut(s) 1008, 1151
TfiI GAWTC 3 cut(s) 101, 198, 1018
Tru1I TTAA 2 cut(s) 723, 819
Tru9I TTAA 2 cut(s) 723, 819
TscAI CASTG 2 cut(s) 155, 1219
TseFI GTSAC 4 cut(s) 31, 373, 650, 783
TseI GCWGC 4 cut(s) 419, 775, 872, 1002
Tsp45I GTSAC 4 cut(s) 31, 373, 650, 783
TspDTI ATGAA 8 cut(s) 17, 41, 261, 674, 707, 741, 963, 1031
TspRI CASTG 2 cut(s) 155, 1219
VneI GTGCAC 1 cut(s) 408
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 5 cut(s) 78, 206, 821, 968, 1058
XceI RCATGY 1 cut(s) 393
XmiI GTMKAC 1 cut(s) 1139
XspI CTAG 3 cut(s) 330, 917, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.