MD13G1105700.v1.1

D-cysteine desulfhydrase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002795
Physical Location & Seq
Reverse (-)
7588343 .. 7590877
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1105700.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGAGGATGAAAGTTGAATGCTTTACCAGTAAGAGCGCGATTTCGGCAGCAATTAACCGCGGCTTTCACTCCGGCGTCGTTTCGAAAGGCCGTCGGATTCAGTGTGTTTCCAATTTGAATTTGAGTGGTGGAGATTTTGTGTCAAAATTGCTTGATAGAAGATGGGCATTGCCTGCCCCTGATGCCAAAATTCACCAAATAGTGTTTTCTAGCAACAAAGTAGCAGAGCCAGGCAAAGCCTTGTCTGATGTTTCCTTCTCCAACATTACCAACCCATCTTTTATGATGAGCAAGAATGATCAAGAACCGTCTTTTTTTGTTGTGAGGGATGATTTGTTGCATCCTTTGATAAATGGTAATAAGGCGCGAAAGTTGGACGCGCTGATTCCCCTCGTGGAAGATCATTCCGTGACTGATGTGGTCACATGCGGAGGCTGTCAAAGCGCACATACAGCAGCTGTTGCTGTTTCATGTTCAGAGAGAGGACTAAAACCACACTTGCTTCTACGAGGGGAGCAGCCAGAAATACTGACCGGCTATAACTTGATTTCAACAGTGTATGGCAGTGTCACATATGTTCCGAGATCTCTGTATGCCAACAGGGAAAAGATGCTAAAGAGCCATGCTGATGATTTGGCAGGCAGTAGTAGTAGTGTCGTATGGTTTGATGATATTTTGGAGGCTTCTTTGAGAGAAGATGATGGTTCTCAGAATTTTTTGCAAAAGGATGCTTGGAGGAGTGACCGTCCTCGTAAAATTGCAATTGTTAACGAAGGTGCAGGAGATGTTGTAGCATTACTAGGTCTGATTCGCCTAGTGCAGTACTTATCCCAGAATCACTTACTTGGGAAAGAGAGGCCCTTGAGATTTGTTGTAGATTCTGGAACTGGGACAACTGCTGTTGGTTTAGCACTTGGAGCCATATGTTTGGGGCTTCCATGGGAGGTTACCGCAGTGATGCTAGCTGATACAGTTGACGGGTATAAACATCAGGAGAAGCGCTTGATTTCCGATTTCAAGAGGCATTTTGGTTCTCAAATTGACAGCTGCTTCGATGAAATGGATAAAGGAATTGTGCACTGGGTAGAACGTCGCCATCCAAGAAAGTTTGGCAATGTTTTAGAAGGGGAAGTAGAGGCATGCCAACACATTGCACAACAAACCGGTATTTTGGTGGATCCAGTGTATACACTCGCTGCGTGGGAGATGGCAACAATCCTTCGTGAACAGGAAGCAGAAGGAGGTGCAACGGTGGTGATGATTCACACTGGAGGCACTCTAGGCTTGTTTGGATTAGCACAGAGGTACAGATCTTACTTCTCTAACCTCAAAACTGGACTGTCCAAGTCGAGAGGGAGTTGA

Protein Analysis

454

Amino Acids

49.68

Weight (kDa)

7.66

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 103 - 424 1.2e-09 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1187
AccII CGCG 4 cut(s) 38, 60, 367, 380
AciI CCGC 4 cut(s) 58, 60, 429, 951
AclWI GGATC 2 cut(s) 1172, 1185
AcsI RAATTY 3 cut(s) 118, 189, 712
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 824, 1307
AfeI AGCGCT 1 cut(s) 1001
AgeI ACCGGT 1 cut(s) 1163
AgsI TTSAA 4 cut(s) 17, 118, 552, 1018
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 3 cut(s) 458, 965, 1047
AluI AGCT 3 cut(s) 458, 965, 1047
Alw21I GWGCWC 1 cut(s) 1080
Alw44I GTGCAC 1 cut(s) 1076
AlwI GGATC 2 cut(s) 1172, 1185
AlwNI CAGNNNCTG 1 cut(s) 458
Aor51HI AGCGCT 1 cut(s) 1001
AoxI GGCC 2 cut(s) 88, 857
ApaLI GTGCAC 1 cut(s) 1076
ApeKI GCWGC 5 cut(s) 47, 455, 517, 1047, 1196
ApoI RAATTY 3 cut(s) 118, 189, 712
ArsI GACNNNNNNTTYG 2 cut(s) 1034, 1066
AsiGI ACCGGT 1 cut(s) 1163
AspLEI GCGC 5 cut(s) 38, 367, 382, 446, 1002
AspS9I GGNCC 1 cut(s) 858
AsuHPI GGTGA 2 cut(s) 185, 1267
AsuII TTCGAA 1 cut(s) 83
AsuNHI GCTAGC 1 cut(s) 961
BaeGI GKGCMC 1 cut(s) 1080
BamHI GGATCC 1 cut(s) 1177
BauI CACGAG 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 1080
BbvI GCAGC 5 cut(s) 59, 467, 529, 1034, 1183
BccI CCATC 5 cut(s) 156, 283, 695, 1104, 1201
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 231
BclI TGATCA 1 cut(s) 298
BfaI CTAG 5 cut(s) 210, 800, 815, 962, 1280
BfoI RGCGCY 1 cut(s) 1003
BglII AGATCT 2 cut(s) 584, 1310
BisI GCNGC 6 cut(s) 48, 61, 456, 518, 1048, 1197
BlsI GCNGC 6 cut(s) 49, 62, 457, 519, 1049, 1198
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 858
BmiI GGNNCC 2 cut(s) 919, 1179
BmrFI CCNGG 1 cut(s) 231
BmrI ACTGGG 2 cut(s) 897, 1090
BmsI GCATC 5 cut(s) 172, 349, 600, 718, 948
BmtI GCTAGC 1 cut(s) 965
BmuI ACTGGG 2 cut(s) 897, 1090
BpmI CTGGAG 1 cut(s) 1290
Bpu14I TTCGAA 1 cut(s) 83
BpuEI CTTGAG 1 cut(s) 883
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 58, 938
BsaWI WCCGGW 1 cut(s) 1163
BsaXI ACNNNNNCTCC 6 cut(s) 123, 153, 730, 760, 774, 804
Bse118I RCCGGY 2 cut(s) 533, 1163
Bse1I ACTGG 6 cut(s) 27, 892, 1085, 1181, 1273, 1339
Bse3DI GCAATG 3 cut(s) 167, 1120, 1149
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 58, 938
BseGI GGATG 5 cut(s) 12, 334, 340, 733, 1096
BseMI GCAATG 3 cut(s) 167, 1120, 1149
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 6 cut(s) 27, 892, 1085, 1181, 1273, 1339
BseRI GAGGAG 1 cut(s) 751
BseSI GKGCMC 1 cut(s) 1080
BseXI GCAGC 5 cut(s) 59, 467, 529, 1034, 1183
BsgI GTGCAG 2 cut(s) 798, 839
Bsh1236I CGCG 4 cut(s) 38, 60, 367, 380
BshFI GGCC 2 cut(s) 90, 859
BshTI ACCGGT 1 cut(s) 1163
BsiHKAI GWGCWC 1 cut(s) 1080
BsiSI CCGG 3 cut(s) 72, 534, 1164
BslFI GGGAC 1 cut(s) 904
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 2 cut(s) 90, 859
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 1080
Bsp143I GATC 5 cut(s) 298, 400, 584, 1177, 1310
Bsp19I CCATGG 1 cut(s) 938
BspACI CCGC 4 cut(s) 58, 60, 429, 951
BspANI GGCC 2 cut(s) 90, 859
BspCNI CTCAG 1 cut(s) 721
BspFNI CGCG 4 cut(s) 38, 60, 367, 380
BspLI GGNNCC 2 cut(s) 919, 1179
BspOI GCTAGC 1 cut(s) 965
BspPI GGATC 2 cut(s) 1172, 1185
BspT104I TTCGAA 1 cut(s) 83
BsrDI GCAATG 3 cut(s) 167, 1120, 1149
BsrFI RCCGGY 2 cut(s) 533, 1163
BsrI ACTGG 6 cut(s) 27, 892, 1085, 1181, 1273, 1339
BssAI RCCGGY 2 cut(s) 533, 1163
BssECI CCNNGG 2 cut(s) 58, 938
BssMI GATC 5 cut(s) 298, 400, 584, 1177, 1310
BssNAI GTATAC 1 cut(s) 1188
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 392
BssT1I CCWWGG 1 cut(s) 938
Bst1107I GTATAC 1 cut(s) 1188
Bst2BI CACGAG 1 cut(s) 392
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 6 cut(s) 309, 556, 746, 973, 1252, 1341
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 2 cut(s) 173, 461
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 4 cut(s) 174, 640, 963, 1141
BstDEI CTNAG 1 cut(s) 708
BstDSI CCRYGG 2 cut(s) 58, 938
BstEII GGTNACC 1 cut(s) 946
BstF5I GGATG 5 cut(s) 12, 334, 340, 733, 1096
BstFNI CGCG 4 cut(s) 38, 60, 367, 380
BstH2I RGCGCY 1 cut(s) 1003
BstHHI GCGC 5 cut(s) 38, 367, 382, 446, 1002
BstKTI GATC 5 cut(s) 301, 403, 587, 1180, 1313
BstMBI GATC 5 cut(s) 298, 400, 584, 1177, 1310
BstMWI GCNNNNNNNGC 8 cut(s) 44, 173, 182, 441, 452, 461, 917, 1281
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 2 cut(s) 429, 1143
BstPI GGTNACC 1 cut(s) 946
BstSCI CCNGG 1 cut(s) 229
BstSLI GKGCMC 1 cut(s) 1080
BstUI CGCG 4 cut(s) 38, 60, 367, 380
BstV1I GCAGC 5 cut(s) 59, 467, 529, 1034, 1183
BstX2I RGATCY 3 cut(s) 584, 1177, 1310
BstXI CCANNNNNNTGG 1 cut(s) 928
BstYI RGATCY 3 cut(s) 584, 1177, 1310
BstZ17I GTATAC 1 cut(s) 1188
BsuRI GGCC 2 cut(s) 90, 859
BtgI CCRYGG 2 cut(s) 58, 938
BtsCI GGATG 5 cut(s) 12, 334, 340, 733, 1096
BtsI GCAGTG 2 cut(s) 571, 960
BtsIMutI CAGTG 7 cut(s) 107, 561, 571, 960, 1078, 1188, 1266
Cac8I GCNNGC 4 cut(s) 174, 640, 963, 1141
CaiI CAGNNNCTG 1 cut(s) 458
CfoI GCGC 5 cut(s) 38, 367, 382, 446, 1002
Cfr10I RCCGGY 2 cut(s) 533, 1163
Cfr13I GGNCC 1 cut(s) 858
Cfr42I CCGCGG 1 cut(s) 61
CseI GACGC 2 cut(s) 64, 386
Csp6I GTAC 2 cut(s) 823, 1306
CspAI ACCGGT 1 cut(s) 1163
CviAII CATG 5 cut(s) 426, 471, 623, 939, 1140
CviQI GTAC 2 cut(s) 823, 1306
DdeI CTNAG 1 cut(s) 708
DpnI GATC 5 cut(s) 300, 402, 586, 1179, 1312
DpnII GATC 5 cut(s) 298, 400, 584, 1177, 1310
Eco130I CCWWGG 1 cut(s) 938
Eco47III AGCGCT 1 cut(s) 1001
Eco91I GGTNACC 1 cut(s) 946
EcoO109I RGGNCCY 1 cut(s) 858
EcoO65I GGTNACC 1 cut(s) 946
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 938
ErhI CCWWGG 1 cut(s) 938
FaeI CATG 5 cut(s) 429, 474, 626, 942, 1143
FaqI GGGAC 1 cut(s) 904
FatI CATG 5 cut(s) 425, 470, 622, 938, 1139
FauNDI CATATG 2 cut(s) 574, 923
FbaI TGATCA 1 cut(s) 298
FblI GTMKAC 1 cut(s) 1187
Fnu4HI GCNGC 6 cut(s) 48, 61, 456, 518, 1048, 1197
FokI GGATG 5 cut(s) 19, 327, 341, 740, 1083
Fsp4HI GCNGC 6 cut(s) 48, 61, 456, 518, 1048, 1197
FspBI CTAG 5 cut(s) 210, 800, 815, 962, 1280
GlaI GCGC 5 cut(s) 37, 366, 381, 445, 1001
GluI GCNGC 6 cut(s) 48, 61, 456, 518, 1048, 1197
GsuI CTGGAG 1 cut(s) 1290
HaeII RGCGCY 1 cut(s) 1003
HaeIII GGCC 2 cut(s) 90, 859
HapII CCGG 3 cut(s) 72, 534, 1164
HgaI GACGC 2 cut(s) 64, 386
HhaI GCGC 5 cut(s) 38, 367, 382, 446, 1002
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 5 cut(s) 429, 474, 626, 942, 1143
Hin6I GCGC 5 cut(s) 36, 365, 380, 444, 1000
HinP1I GCGC 5 cut(s) 36, 365, 380, 444, 1000
HincII GTYRAC 2 cut(s) 769, 976
HindII GTYRAC 2 cut(s) 769, 976
HinfI GANTC 6 cut(s) 97, 385, 808, 835, 878, 1261
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 3 cut(s) 72, 534, 1164
HphI GGTGA 2 cut(s) 185, 1267
Hpy166II GTNNAC 5 cut(s) 769, 976, 1078, 1188, 1226
Hpy188I TCNGA 7 cut(s) 96, 247, 478, 582, 711, 807, 1012
Hpy188III TCNNGA 6 cut(s) 302, 882, 992, 1018, 1223, 1350
Hpy8I GTNNAC 5 cut(s) 769, 976, 1078, 1188, 1226
Hpy99I CGWCG 3 cut(s) 80, 96, 1095
HpyAV CCTTC 5 cut(s) 265, 767, 1118, 1229, 1232
HpyCH4III ACNGT 6 cut(s) 309, 556, 746, 973, 1252, 1341
HpyCH4IV ACGT 1 cut(s) 1090
HpyCH4V TGCA 8 cut(s) 340, 721, 761, 779, 820, 1078, 1154, 1247
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 173, 182, 441, 452, 461, 917, 1281
HpyF3I CTNAG 1 cut(s) 708
HpySE526I ACGT 1 cut(s) 1090
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 5 cut(s) 429, 474, 626, 942, 1143
HspAI GCGC 5 cut(s) 36, 365, 380, 444, 1000
Ksp22I TGATCA 1 cut(s) 298
KspAI GTTAAC 1 cut(s) 769
KspI CCGCGG 1 cut(s) 61
Kzo9I GATC 5 cut(s) 298, 400, 584, 1177, 1310
LmnI GCTCC 2 cut(s) 514, 917
Lsp1109I GCAGC 5 cut(s) 59, 467, 529, 1034, 1183
LweI GCATC 5 cut(s) 172, 349, 600, 718, 948
MaeI CTAG 5 cut(s) 210, 800, 815, 962, 1280
MaeII ACGT 1 cut(s) 1090
MaeIII GTNAC 5 cut(s) 409, 421, 568, 740, 946
MalI GATC 5 cut(s) 300, 402, 586, 1179, 1312
MboI GATC 5 cut(s) 298, 400, 584, 1177, 1310
MboII GAAGA 3 cut(s) 171, 410, 707
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 3 cut(s) 584, 1177, 1310
MhlI GDGCHC 1 cut(s) 1080
MmeI TCCRAC 3 cut(s) 74, 285, 354
MseI TTAA 2 cut(s) 54, 768
MslI CAYNNNNRTG 1 cut(s) 627
MspA1I CMGCKG 3 cut(s) 60, 458, 1047
MspI CCGG 3 cut(s) 72, 534, 1164
MspR9I CCNGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 4 cut(s) 38, 60, 367, 380
MwoI GCNNNNNNNGC 8 cut(s) 44, 173, 182, 441, 452, 461, 917, 1281
NcoI CCATGG 1 cut(s) 938
NdeI CATATG 2 cut(s) 574, 923
NdeII GATC 5 cut(s) 298, 400, 584, 1177, 1310
NheI GCTAGC 1 cut(s) 961
NlaIII CATG 5 cut(s) 429, 474, 626, 942, 1143
NlaIV GGNNCC 2 cut(s) 919, 1179
NmuCI GTSAC 4 cut(s) 409, 421, 568, 740
NspI RCATGY 2 cut(s) 429, 1143
NspV TTCGAA 1 cut(s) 83
PaeI GCATGC 1 cut(s) 1143
PctI GAATGC 1 cut(s) 23
PfeI GAWTC 6 cut(s) 97, 385, 808, 835, 878, 1261
PinAI ACCGGT 1 cut(s) 1163
PkrI GCNGC 6 cut(s) 49, 62, 457, 519, 1049, 1198
Psp6I CCWGG 1 cut(s) 229
PspEI GGTNACC 1 cut(s) 946
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 919, 1179
PspPI GGNCC 1 cut(s) 858
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 3 cut(s) 584, 1177, 1310
PvuII CAGCTG 2 cut(s) 458, 1047
RsaI GTAC 2 cut(s) 824, 1307
RsaNI GTAC 2 cut(s) 823, 1306
RseI CAYNNNNRTG 1 cut(s) 627
SacII CCGCGG 1 cut(s) 61
SaqAI TTAA 2 cut(s) 54, 768
SatI GCNGC 6 cut(s) 48, 61, 456, 518, 1048, 1197
Sau3AI GATC 5 cut(s) 298, 400, 584, 1177, 1310
Sau96I GGNCC 1 cut(s) 858
ScaI AGTACT 1 cut(s) 824
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 1 cut(s) 1080
SfaNI GCATC 5 cut(s) 172, 349, 600, 718, 948
Sfr303I CCGCGG 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 1 cut(s) 862
SmoI CTYRAG 1 cut(s) 862
SphI GCATGC 1 cut(s) 1143
SsiI CCGC 4 cut(s) 58, 60, 429, 951
SspMI CTAG 5 cut(s) 210, 800, 815, 962, 1280
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 1 cut(s) 938
TaaI ACNGT 6 cut(s) 309, 556, 746, 973, 1252, 1341
TaiI ACGT 1 cut(s) 1093
TaqI TCGA 3 cut(s) 83, 1053, 1349
TatI WGTACW 1 cut(s) 822
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 6 cut(s) 97, 385, 808, 835, 878, 1261
Tru1I TTAA 2 cut(s) 54, 768
Tru9I TTAA 2 cut(s) 54, 768
TscAI CASTG 7 cut(s) 107, 561, 571, 960, 1085, 1188, 1273
TseFI GTSAC 4 cut(s) 409, 421, 568, 740
TseI GCWGC 5 cut(s) 47, 455, 517, 1047, 1196
Tsp45I GTSAC 4 cut(s) 409, 421, 568, 740
TspDTI ATGAA 3 cut(s) 23, 459, 1071
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 7 cut(s) 107, 561, 571, 960, 1085, 1188, 1273
VneI GTGCAC 1 cut(s) 1076
XapI RAATTY 3 cut(s) 118, 189, 712
XceI RCATGY 2 cut(s) 429, 1143
XmiI GTMKAC 1 cut(s) 1187
XspI CTAG 5 cut(s) 210, 800, 815, 962, 1280
ZrmI AGTACT 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.