RchiOBHm_Chr4g0432431

D-cysteine desulfhydrase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002795
Physical Location & Seq
Forward (+)
56604225 .. 56606980
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40104

Sequence Viewer

Length: 1338 bp
ATGAAAGTTGAAAGCTTTCCCAGTAAGAGAGCCATTTCAGCCTTCAAACGGAGCTTTCATTCCGGCCACGTTTTGAAACAGTGTGTTTCCAATGTGAAACTGAGTAAAGAAGAGTTTGTGTCCAAATTGCTTGATAGAAGATGGACATTGCCTAGCCCTGATGCTAAAATTCACCAAATAATGCTTTCAACCAGCAAAGTACAACCACAACCAGGCAGACCCTTGTCTGATATTTCCTTCTGGAACAATACTAACCCATGTTTTAGTCATTCTATGGCAGAAAAGGACGATTCTTTCTTTATTGTGAGGGATGATTTGTTGCATCCGTTGATAAATGGGAATAAAGCAAGAAAATTGGATGCATTGATTCCCCTTGTTGAAGATCATTCCGTGACTGATGTGGTGACATGTGGAGGTTGTCAAAGTGCCCATACAGCAGCTGTTGCCGTTTCATGTTCAGAAAGAGGATTAAAACCGCATTTACTTCTACGAGGGGAGCAGCCTGAAATACTGACAGGCTATAACTTGATGTCAACAATATATGGCAATGTCACATATGTTCCAAGATCGCTTTATGCCAATAGGGAAAAGATGTTAAAGAGCCATGCTGATTATTTGGCAGGCAGTAGCGGTAACGTCCTTCGGTTTGATGATATTTTGGAGGCCTCTTTGAATCAAAGTGATGGTGCTCTGAATCTTTTGCAAAAGGATGCTAATAGAAGTGACCATCCTCGGAAAATTGTAATTGTCAATGAAGGCGCAGGAGATGTTGTAGCATTACTAGGTTTGATTCGCCTAGCGCAGTACTTATCCCAGAATCACTTACTTGGAAAAGAGAGGGTCTTGAAGTTTGTTGTAGATTCTGGAACTGGGACAACAGCTGTTGGTTTAGCTCTTGGAGCCATATGTTTGGGGCTCCCATGGGAGGTAACTGCAGTGATGTTGGCTGATTCATATGATGGGTACAGACATCAGGAGAAGCGCTTGATATCCAATTTTAAGAGGCATTTTGATACTCATATTGATCACTGCTTCGATGATATAGACAAAGGAATTGTGCACTGGGTAGAACGTTGCCACCCGAGAAAATTTGGCAATGTATTGGAAGGGGAATTAGAGGCATGCCAACATATTGCACAACAAACTGGTATTTTAGTCGACCCAGTTTACACACTTTCTGCTTGGGAGATGGCAATGGCCCTTCATAAAAAAGAAGCAGAAGTTGAAGGAGGTGTACAAGTGGTCATGCTCCACACTGGAGGCACTCTAGGCATGTTTGGATTAGCACAGAGGTACAAATCTTACTTCTCCAAGCTCAAAGTTGGATCATCCAGTTAA

Protein Analysis

445

Amino Acids

49.3

Weight (kDa)

8.03

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 100 - 420 2.5e-09 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1158
AciI CCGC 2 cut(s) 476, 630
AclI AACGTT 1 cut(s) 1072
AclWI GGATC 1 cut(s) 1333
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 2 cut(s) 168, 1088
AfaI GTAC 5 cut(s) 201, 806, 965, 1236, 1295
AfeI AGCGCT 1 cut(s) 983
AfiI CCNNNNNNNGG 3 cut(s) 48, 212, 925
AflIII ACRYGT 1 cut(s) 407
AgsI TTSAA 8 cut(s) 11, 46, 76, 189, 380, 673, 847, 1226
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 6 cut(s) 15, 54, 440, 881, 893, 1315
AluI AGCT 6 cut(s) 15, 54, 440, 881, 893, 1315
Alw21I GWGCWC 2 cut(s) 691, 1062
Alw44I GTGCAC 1 cut(s) 1058
AlwI GGATC 1 cut(s) 1333
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 1 cut(s) 1081
Aor51HI AGCGCT 1 cut(s) 983
AoxI GGCC 3 cut(s) 64, 663, 1197
ApaLI GTGCAC 1 cut(s) 1058
ApeKI GCWGC 2 cut(s) 437, 499
ApoI RAATTY 2 cut(s) 168, 1088
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 3 cut(s) 761, 802, 984
AspS9I GGNCC 1 cut(s) 1198
AsuHPI GGTGA 2 cut(s) 164, 415
AvaI CYCGRG 1 cut(s) 1081
BaeGI GKGCMC 2 cut(s) 430, 1062
BanII GRGCYC 1 cut(s) 918
BarI GAAGNNNNNNTAC 2 cut(s) 1218, 1250
Bbv12I GWGCWC 2 cut(s) 691, 1062
BbvI GCAGC 2 cut(s) 449, 511
BccI CCATC 5 cut(s) 135, 677, 735, 953, 1183
BceAI ACGGC 1 cut(s) 431
BciT130I CCWGG 1 cut(s) 213
BclI TGATCA 1 cut(s) 1024
BfaI CTAG 4 cut(s) 153, 782, 797, 1268
BfmI CTRYAG 1 cut(s) 933
BfoI RGCGCY 1 cut(s) 985
BisI GCNGC 2 cut(s) 438, 500
BlsI GCNGC 2 cut(s) 439, 501
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 1 cut(s) 1198
BmiI GGNNCC 2 cut(s) 901, 917
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 4 cut(s) 15, 879, 1072, 1157
BmsI GCATC 4 cut(s) 151, 331, 349, 700
BmuI ACTGGG 4 cut(s) 15, 879, 1072, 1157
BoxI GACNNNNGTC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 1278
BsaJI CCNNGG 2 cut(s) 731, 920
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 212, 925
Bse1I ACTGG 7 cut(s) 21, 874, 1067, 1150, 1163, 1261, 1332
Bse3DI GCAATG 4 cut(s) 146, 553, 1102, 1200
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 731, 920
BseGI GGATG 6 cut(s) 316, 322, 364, 715, 727, 1328
BseLI CCNNNNNNNGG 3 cut(s) 48, 212, 925
BseMI GCAATG 4 cut(s) 146, 553, 1102, 1200
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 7 cut(s) 21, 874, 1067, 1150, 1163, 1261, 1332
BseSI GKGCMC 2 cut(s) 430, 1062
BseXI GCAGC 2 cut(s) 449, 511
BshFI GGCC 3 cut(s) 66, 665, 1199
BsiHKAI GWGCWC 2 cut(s) 691, 1062
BsiHKCI CYCGRG 1 cut(s) 1081
BsiSI CCGG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 886
BslI CCNNNNNNNGG 3 cut(s) 48, 212, 925
BsmFI GGGAC 1 cut(s) 886
BsnI GGCC 3 cut(s) 66, 665, 1199
BsoBI CYCGRG 1 cut(s) 1081
Bsp1286I GDGCHC 4 cut(s) 430, 691, 918, 1062
Bsp1407I TGTACA 1 cut(s) 1234
Bsp143I GATC 4 cut(s) 382, 566, 1024, 1325
Bsp19I CCATGG 1 cut(s) 920
BspACI CCGC 2 cut(s) 476, 630
BspANI GGCC 3 cut(s) 66, 665, 1199
BspCNI CTCAG 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 901, 917
BspMAI CTGCAG 1 cut(s) 937
BspPI GGATC 1 cut(s) 1333
BsrDI GCAATG 4 cut(s) 146, 553, 1102, 1200
BsrGI TGTACA 1 cut(s) 1234
BsrI ACTGG 7 cut(s) 21, 874, 1067, 1150, 1163, 1261, 1332
BssECI CCNNGG 2 cut(s) 731, 920
BssMI GATC 4 cut(s) 382, 566, 1024, 1325
BssT1I CCWWGG 1 cut(s) 920
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 81
Bst6I CTCTTC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 1234
BstC8I GCNNGC 2 cut(s) 622, 1123
BstDEI CTNAG 1 cut(s) 101
BstDSI CCRYGG 1 cut(s) 920
BstF5I GGATG 6 cut(s) 316, 322, 364, 715, 727, 1328
BstH2I RGCGCY 1 cut(s) 985
BstHHI GCGC 3 cut(s) 761, 802, 984
BstKTI GATC 4 cut(s) 385, 569, 1027, 1328
BstMBI GATC 4 cut(s) 382, 566, 1024, 1325
BstMWI GCNNNNNNNGC 5 cut(s) 38, 434, 443, 899, 1269
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 3 cut(s) 411, 1125, 1276
BstPAI GACNNNNGTC 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 2 cut(s) 430, 1062
BstV1I GCAGC 2 cut(s) 449, 511
BstXI CCANNNNNNTGG 1 cut(s) 910
BsuRI GGCC 3 cut(s) 66, 665, 1199
BtgI CCRYGG 1 cut(s) 920
BtsCI GGATG 6 cut(s) 316, 322, 364, 715, 727, 1328
BtsI GCAGTG 2 cut(s) 942, 1027
BtsIMutI CAGTG 5 cut(s) 86, 942, 1027, 1060, 1254
Cac8I GCNNGC 2 cut(s) 622, 1123
CaiI CAGNNNCTG 1 cut(s) 440
CfoI GCGC 3 cut(s) 761, 802, 984
Cfr13I GGNCC 1 cut(s) 1198
Csp6I GTAC 5 cut(s) 200, 805, 964, 1235, 1294
CviAII CATG 8 cut(s) 258, 408, 453, 605, 921, 1122, 1246, 1273
CviQI GTAC 5 cut(s) 200, 805, 964, 1235, 1294
DdeI CTNAG 1 cut(s) 101
DpnI GATC 4 cut(s) 384, 568, 1026, 1327
DpnII GATC 4 cut(s) 382, 566, 1024, 1325
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 920
Eco147I AGGCCT 1 cut(s) 665
Eco24I GRGCYC 1 cut(s) 918
Eco32I GATATC 1 cut(s) 990
Eco47III AGCGCT 1 cut(s) 983
Eco88I CYCGRG 1 cut(s) 1081
EcoRII CCWGG 1 cut(s) 211
EcoRV GATATC 1 cut(s) 990
EcoT14I CCWWGG 1 cut(s) 920
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 1 cut(s) 920
FaeI CATG 8 cut(s) 261, 411, 456, 608, 924, 1125, 1249, 1276
FaqI GGGAC 1 cut(s) 886
FatI CATG 8 cut(s) 257, 407, 452, 604, 920, 1121, 1245, 1272
FauNDI CATATG 3 cut(s) 556, 905, 955
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 1 cut(s) 1158
Fnu4HI GCNGC 2 cut(s) 438, 500
FokI GGATG 6 cut(s) 309, 323, 371, 714, 722, 1315
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 2 cut(s) 438, 500
FspBI CTAG 4 cut(s) 153, 782, 797, 1268
GlaI GCGC 3 cut(s) 760, 801, 983
GluI GCNGC 2 cut(s) 438, 500
GsuI CTGGAG 1 cut(s) 1278
HaeII RGCGCY 1 cut(s) 985
HaeIII GGCC 3 cut(s) 66, 665, 1199
HapII CCGG 1 cut(s) 63
HhaI GCGC 3 cut(s) 761, 802, 984
Hin1II CATG 8 cut(s) 261, 411, 456, 608, 924, 1125, 1249, 1276
Hin6I GCGC 3 cut(s) 759, 800, 982
HinP1I GCGC 3 cut(s) 759, 800, 982
HincII GTYRAC 2 cut(s) 534, 1159
HindII GTYRAC 2 cut(s) 534, 1159
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 8 cut(s) 290, 367, 673, 694, 790, 817, 860, 950
HpaII CCGG 1 cut(s) 63
HphI GGTGA 2 cut(s) 164, 415
Hpy166II GTNNAC 5 cut(s) 534, 1060, 1159, 1168, 1235
Hpy188I TCNGA 4 cut(s) 229, 460, 693, 735
Hpy188III TCNNGA 4 cut(s) 241, 844, 864, 974
Hpy8I GTNNAC 5 cut(s) 534, 1060, 1159, 1168, 1235
HpyAV CCTTC 7 cut(s) 52, 247, 650, 749, 1100, 1211, 1220
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4IV ACGT 3 cut(s) 69, 636, 1072
HpyCH4V TGCA 6 cut(s) 322, 362, 703, 935, 1060, 1136
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 434, 443, 899, 1269
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 3 cut(s) 69, 636, 1072
Hsp92II CATG 8 cut(s) 261, 411, 456, 608, 924, 1125, 1249, 1276
HspAI GCGC 3 cut(s) 759, 800, 982
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 4 cut(s) 382, 566, 1024, 1325
LmnI GCTCC 5 cut(s) 51, 496, 899, 921, 1254
Lsp1109I GCAGC 2 cut(s) 449, 511
LweI GCATC 4 cut(s) 151, 331, 349, 700
MaeI CTAG 4 cut(s) 153, 782, 797, 1268
MaeII ACGT 3 cut(s) 69, 636, 1072
MaeIII GTNAC 6 cut(s) 391, 403, 550, 632, 722, 928
MalI GATC 4 cut(s) 384, 568, 1026, 1327
MboI GATC 4 cut(s) 382, 566, 1024, 1325
MboII GAAGA 3 cut(s) 122, 150, 392
MhlI GDGCHC 4 cut(s) 430, 691, 918, 1062
MluCI AATT 9 cut(s) 125, 168, 353, 738, 744, 994, 1053, 1088, 1112
MmeI TCCRAC 1 cut(s) 1303
Mph1103I ATGCAT 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 4 cut(s) 470, 596, 999, 1336
MspA1I CMGCKG 2 cut(s) 440, 881
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 5 cut(s) 38, 434, 443, 899, 1269
NcoI CCATGG 1 cut(s) 920
NdeI CATATG 3 cut(s) 556, 905, 955
NdeII GATC 4 cut(s) 382, 566, 1024, 1325
NlaIII CATG 8 cut(s) 261, 411, 456, 608, 924, 1125, 1249, 1276
NlaIV GGNNCC 2 cut(s) 901, 917
NmuCI GTSAC 4 cut(s) 391, 403, 550, 722
NsiI ATGCAT 1 cut(s) 364
NspI RCATGY 3 cut(s) 411, 1125, 1276
PaeI GCATGC 1 cut(s) 1125
PceI AGGCCT 1 cut(s) 665
PciI ACATGT 1 cut(s) 407
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 8 cut(s) 290, 367, 673, 694, 790, 817, 860, 950
PkrI GCNGC 2 cut(s) 439, 501
PscI ACATGT 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 223
Psp1406I AACGTT 1 cut(s) 1072
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 901, 917
PspPI GGNCC 1 cut(s) 1198
PstI CTGCAG 1 cut(s) 937
PstNI CAGNNNCTG 1 cut(s) 440
PvuII CAGCTG 2 cut(s) 440, 881
RsaI GTAC 5 cut(s) 201, 806, 965, 1236, 1295
RsaNI GTAC 5 cut(s) 200, 805, 964, 1235, 1294
SalI GTCGAC 1 cut(s) 1157
SaqAI TTAA 4 cut(s) 470, 596, 999, 1336
SatI GCNGC 2 cut(s) 438, 500
Sau3AI GATC 4 cut(s) 382, 566, 1024, 1325
Sau96I GGNCC 1 cut(s) 1198
ScaI AGTACT 1 cut(s) 806
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 4 cut(s) 430, 691, 918, 1062
SfaNI GCATC 4 cut(s) 151, 331, 349, 700
SfcI CTRYAG 1 cut(s) 933
SphI GCATGC 1 cut(s) 1125
Sse9I AATT 9 cut(s) 125, 168, 353, 738, 744, 994, 1053, 1088, 1112
SseBI AGGCCT 1 cut(s) 665
SsiI CCGC 2 cut(s) 476, 630
SspMI CTAG 4 cut(s) 153, 782, 797, 1268
StuI AGGCCT 1 cut(s) 665
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 920
TaaI ACNGT 1 cut(s) 81
TaiI ACGT 3 cut(s) 72, 639, 1075
TaqI TCGA 2 cut(s) 1035, 1158
TasI AATT 9 cut(s) 125, 168, 353, 738, 744, 994, 1053, 1088, 1112
TatI WGTACW 3 cut(s) 199, 804, 1234
TfiI GAWTC 8 cut(s) 290, 367, 673, 694, 790, 817, 860, 950
Tru1I TTAA 4 cut(s) 470, 596, 999, 1336
Tru9I TTAA 4 cut(s) 470, 596, 999, 1336
TscAI CASTG 5 cut(s) 86, 942, 1034, 1067, 1261
TseFI GTSAC 4 cut(s) 391, 403, 550, 722
TseI GCWGC 2 cut(s) 437, 499
Tsp45I GTSAC 4 cut(s) 391, 403, 550, 722
TspDTI ATGAA 6 cut(s) 17, 47, 441, 768, 942, 1193
TspGWI ACGGA 3 cut(s) 64, 315, 379
TspRI CASTG 5 cut(s) 86, 942, 1034, 1067, 1261
VneI GTGCAC 1 cut(s) 1058
XapI RAATTY 2 cut(s) 168, 1088
XceI RCATGY 3 cut(s) 411, 1125, 1276
XmiI GTMKAC 1 cut(s) 1158
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 4 cut(s) 153, 782, 797, 1268
ZrmI AGTACT 1 cut(s) 806
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.