Rh4AG314200

D-cysteine desulfhydrase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002795
Physical Location & Seq
Forward (+)
62440305 .. 62442840
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG314200.1

Sequence Viewer

Length: 1344 bp
ATGAGCATGAAAGTTGAAAGCTTTCCCAGTAAGAGAACCATTTCAGCATTCAAACGGAGCTTTCATTCCGGCCACGTTTTGAAACAGTGTGTTTCCAATGTGAAACTGAGTAAAGAAGAGTTTGTGTCCAAATTGCTTGATAGAAGATGGACATTGCCTATCCCTGATGCTAAAATTCACCAAATAAAGCTTTCAACCAGCAAAGTACAACCACAACCAGGCAGACCCTTGTCTGATATTTCCTTCTGGAACAATACTAACCCATGTTTTAGTCATTCTATGGCGGAAAAGGACGATTCTTTCTTTATTGTGAGGGATGATTTGTTGCATCCGTTGATAAATGGGAATAAAGCAAGAAAATTGGATGCATTGATTCCCCTTGTTGAAGATCATTCCGTGACTGATGTGGTGACATGTGGAGGTTGTCAAAGTGCCCATACAGCAGCTGTTGCCGTTTCATGTTCAGAAAGAGGATTAAAACCACATTTACTTCTACGAGGGGAGCAGCCTGAAATACTGACAGGCTATAACTTGATGTCAACAATATATGGCAATGTCACATATGTTCCAAGATCGCTTTATGCCAATAGGGAAAAGATGTTAAAGAGCCATGCTGATTATTTGGCAGGCAGTAGTGGTAACGTCCTTTGGTTTGATGATATTTTGGAGGCTTCTTTGAATCAAAGTGATGGTGCTCTGAATCTTTTGCAAAAGGATGCTAATAGAAGTGACCATCCTCGGAAAATTGTAATTGTCAATGAAGGCGCAGGAGATGTTGTAGCATTACTAGGTTTGATTCGCCTAGTGCAGTACTTATCCCAGAATCACTTACTTGGAAAAGAGAGGGTCTTGAAGTTTGTTGTAGATTCTGGAACTGGGACAACAGCTGTTGGTTTAGCTCTTGGAGCCATATGTTTGGGGCTCCCATGGGAGGTAACTGCAGTGATGTTGGCTGATTCATATGATGGGTACAGACATCAGGAGAAGCGCTTGATATCCAATTTTAAGAGGCATTTTGATATTCATATTGATCACTGCTTTGATGATATAGACAAAGGAATTGTGCACTGGGTAGAACGTTGCCACCCGAGAAAATTTGGCAATGTATTGGAAGGGGAATTAGAGGCATGCCAACATATTGCACAACAAACTGGTATTTTAGTCGACCCAGTTTACACACTTTCTGCTTGGGAGATGGCAATGGCCCTTCATAAAAAGGAAGCAGAAGTTGGAGGAGGTGTAAAAGTGGTCATGCTCCACAGTGGAGGCACTCTAGGCATGTTTGGATTAGCACAGAGGTACAAATCTTACTTCTCCAAGCTCAAAGTTGGATCATCCAGCTAA

Protein Analysis

447

Amino Acids

49.56

Weight (kDa)

8.45

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 102 - 422 3.1e-08 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1164
AciI CCGC 1 cut(s) 284
AclI AACGTT 1 cut(s) 1078
AclWI GGATC 1 cut(s) 1339
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 2 cut(s) 174, 1094
AfaI GTAC 4 cut(s) 207, 812, 971, 1301
AfeI AGCGCT 1 cut(s) 989
AfiI CCNNNNNNNGG 2 cut(s) 218, 931
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 7 cut(s) 17, 52, 82, 195, 386, 679, 853
AjnI CCWGG 1 cut(s) 217
AjuI GAANNNNNNNTTGG 2 cut(s) 1212, 1244
AluBI AGCT 8 cut(s) 21, 60, 190, 446, 887, 899, 1321, 1341
AluI AGCT 8 cut(s) 21, 60, 190, 446, 887, 899, 1321, 1341
Alw21I GWGCWC 2 cut(s) 697, 1068
Alw44I GTGCAC 1 cut(s) 1064
AlwI GGATC 1 cut(s) 1339
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 1087
Aor51HI AGCGCT 1 cut(s) 989
AoxI GGCC 2 cut(s) 70, 1203
ApaLI GTGCAC 1 cut(s) 1064
ApeKI GCWGC 2 cut(s) 443, 505
ApoI RAATTY 2 cut(s) 174, 1094
Asp700I GAANNNNTTC 2 cut(s) 21, 40
AspLEI GCGC 2 cut(s) 767, 990
AspS9I GGNCC 1 cut(s) 1204
AsuHPI GGTGA 2 cut(s) 170, 421
AvaI CYCGRG 1 cut(s) 1087
BaeGI GKGCMC 2 cut(s) 436, 1068
BanII GRGCYC 1 cut(s) 924
Bbv12I GWGCWC 2 cut(s) 697, 1068
BbvI GCAGC 2 cut(s) 455, 517
BccI CCATC 5 cut(s) 141, 683, 741, 959, 1189
BceAI ACGGC 1 cut(s) 437
BciT130I CCWGG 1 cut(s) 219
BclI TGATCA 1 cut(s) 1030
BfaI CTAG 3 cut(s) 788, 803, 1274
BfmI CTRYAG 1 cut(s) 939
BfoI RGCGCY 1 cut(s) 991
BisI GCNGC 2 cut(s) 444, 506
BlsI GCNGC 2 cut(s) 445, 507
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 1 cut(s) 219
BmeT110I CYCGRG 1 cut(s) 1087
BmgT120I GGNCC 1 cut(s) 1204
BmiI GGNNCC 2 cut(s) 907, 923
BmrFI CCNGG 1 cut(s) 219
BmrI ACTGGG 4 cut(s) 21, 885, 1078, 1163
BmsI GCATC 4 cut(s) 157, 337, 355, 706
BmuI ACTGGG 4 cut(s) 21, 885, 1078, 1163
BoxI GACNNNNGTC 1 cut(s) 229
BsaJI CCNNGG 2 cut(s) 737, 926
BsaXI ACNNNNNCTCC 4 cut(s) 762, 792, 1224, 1254
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 931
Bse1I ACTGG 5 cut(s) 27, 880, 1073, 1156, 1169
Bse3DI GCAATG 4 cut(s) 152, 559, 1108, 1206
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 737, 926
BseGI GGATG 6 cut(s) 322, 328, 370, 721, 733, 1334
BseLI CCNNNNNNNGG 2 cut(s) 218, 931
BseMI GCAATG 4 cut(s) 152, 559, 1108, 1206
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 5 cut(s) 27, 880, 1073, 1156, 1169
BseRI GAGGAG 1 cut(s) 1248
BseSI GKGCMC 2 cut(s) 436, 1068
BseXI GCAGC 2 cut(s) 455, 517
BsgI GTGCAG 1 cut(s) 827
BshFI GGCC 2 cut(s) 72, 1205
BsiHKAI GWGCWC 2 cut(s) 697, 1068
BsiHKCI CYCGRG 1 cut(s) 1087
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 892
BslI CCNNNNNNNGG 2 cut(s) 218, 931
BsmFI GGGAC 1 cut(s) 892
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 2 cut(s) 72, 1205
BsoBI CYCGRG 1 cut(s) 1087
Bsp1286I GDGCHC 4 cut(s) 436, 697, 924, 1068
Bsp143I GATC 4 cut(s) 388, 572, 1030, 1331
Bsp19I CCATGG 1 cut(s) 926
BspACI CCGC 1 cut(s) 284
BspANI GGCC 2 cut(s) 72, 1205
BspCNI CTCAG 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 907, 923
BspMAI CTGCAG 1 cut(s) 943
BspPI GGATC 1 cut(s) 1339
BsrDI GCAATG 4 cut(s) 152, 559, 1108, 1206
BsrI ACTGG 5 cut(s) 27, 880, 1073, 1156, 1169
BssECI CCNNGG 2 cut(s) 737, 926
BssMI GATC 4 cut(s) 388, 572, 1030, 1331
BssT1I CCWWGG 1 cut(s) 926
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 2 cut(s) 87, 1262
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 628, 1129
BstDEI CTNAG 1 cut(s) 107
BstDSI CCRYGG 1 cut(s) 926
BstF5I GGATG 6 cut(s) 322, 328, 370, 721, 733, 1334
BstH2I RGCGCY 1 cut(s) 991
BstHHI GCGC 2 cut(s) 767, 990
BstKTI GATC 4 cut(s) 391, 575, 1033, 1334
BstMBI GATC 4 cut(s) 388, 572, 1030, 1331
BstMWI GCNNNNNNNGC 4 cut(s) 440, 449, 905, 1275
BstNI CCWGG 1 cut(s) 219
BstNSI RCATGY 3 cut(s) 417, 1131, 1282
BstPAI GACNNNNGTC 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 217
BstSFI CTRYAG 1 cut(s) 939
BstSLI GKGCMC 2 cut(s) 436, 1068
BstV1I GCAGC 2 cut(s) 455, 517
BstXI CCANNNNNNTGG 1 cut(s) 916
BsuRI GGCC 2 cut(s) 72, 1205
BtgI CCRYGG 1 cut(s) 926
BtsCI GGATG 6 cut(s) 322, 328, 370, 721, 733, 1334
BtsI GCAGTG 2 cut(s) 948, 1033
BtsIMutI CAGTG 5 cut(s) 92, 948, 1033, 1066, 1267
Cac8I GCNNGC 2 cut(s) 628, 1129
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 2 cut(s) 767, 990
Cfr13I GGNCC 1 cut(s) 1204
Csp6I GTAC 4 cut(s) 206, 811, 970, 1300
CviAII CATG 9 cut(s) 7, 264, 414, 459, 611, 927, 1128, 1252, 1279
CviQI GTAC 4 cut(s) 206, 811, 970, 1300
DdeI CTNAG 1 cut(s) 107
DpnI GATC 4 cut(s) 390, 574, 1032, 1333
DpnII GATC 4 cut(s) 388, 572, 1030, 1331
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 926
Eco24I GRGCYC 1 cut(s) 924
Eco32I GATATC 1 cut(s) 996
Eco47III AGCGCT 1 cut(s) 989
Eco88I CYCGRG 1 cut(s) 1087
EcoRII CCWGG 1 cut(s) 217
EcoRV GATATC 1 cut(s) 996
EcoT14I CCWWGG 1 cut(s) 926
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 1 cut(s) 926
FaeI CATG 9 cut(s) 10, 267, 417, 462, 614, 930, 1131, 1255, 1282
FaqI GGGAC 1 cut(s) 892
FatI CATG 9 cut(s) 6, 263, 413, 458, 610, 926, 1127, 1251, 1278
FauNDI CATATG 3 cut(s) 562, 911, 961
FbaI TGATCA 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 2 cut(s) 444, 506
FokI GGATG 6 cut(s) 315, 329, 377, 720, 728, 1321
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 2 cut(s) 444, 506
FspBI CTAG 3 cut(s) 788, 803, 1274
GlaI GCGC 2 cut(s) 766, 989
GluI GCNGC 2 cut(s) 444, 506
HaeII RGCGCY 1 cut(s) 991
HaeIII GGCC 2 cut(s) 72, 1205
HapII CCGG 1 cut(s) 69
HhaI GCGC 2 cut(s) 767, 990
Hin1II CATG 9 cut(s) 10, 267, 417, 462, 614, 930, 1131, 1255, 1282
Hin6I GCGC 2 cut(s) 765, 988
HinP1I GCGC 2 cut(s) 765, 988
HincII GTYRAC 2 cut(s) 540, 1165
HindII GTYRAC 2 cut(s) 540, 1165
HindIII AAGCTT 2 cut(s) 19, 188
HinfI GANTC 8 cut(s) 296, 373, 679, 700, 796, 823, 866, 956
HpaII CCGG 1 cut(s) 69
HphI GGTGA 2 cut(s) 170, 421
Hpy166II GTNNAC 4 cut(s) 540, 1066, 1165, 1174
Hpy188I TCNGA 4 cut(s) 235, 466, 699, 741
Hpy188III TCNNGA 4 cut(s) 247, 850, 870, 980
Hpy8I GTNNAC 4 cut(s) 540, 1066, 1165, 1174
HpyAV CCTTC 4 cut(s) 253, 755, 1106, 1217
HpyCH4III ACNGT 2 cut(s) 87, 1262
HpyCH4IV ACGT 3 cut(s) 75, 642, 1078
HpyCH4V TGCA 7 cut(s) 328, 368, 709, 808, 941, 1066, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 449, 905, 1275
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 3 cut(s) 75, 642, 1078
Hsp92II CATG 9 cut(s) 10, 267, 417, 462, 614, 930, 1131, 1255, 1282
HspAI GCGC 2 cut(s) 765, 988
Ksp22I TGATCA 1 cut(s) 1030
Kzo9I GATC 4 cut(s) 388, 572, 1030, 1331
LmnI GCTCC 5 cut(s) 57, 502, 905, 927, 1260
Lsp1109I GCAGC 2 cut(s) 455, 517
LweI GCATC 4 cut(s) 157, 337, 355, 706
MaeI CTAG 3 cut(s) 788, 803, 1274
MaeII ACGT 3 cut(s) 75, 642, 1078
MaeIII GTNAC 6 cut(s) 397, 409, 556, 638, 728, 934
MalI GATC 4 cut(s) 390, 574, 1032, 1333
MboI GATC 4 cut(s) 388, 572, 1030, 1331
MboII GAAGA 3 cut(s) 128, 156, 398
MhlI GDGCHC 4 cut(s) 436, 697, 924, 1068
MluCI AATT 9 cut(s) 131, 174, 359, 744, 750, 1000, 1059, 1094, 1118
MmeI TCCRAC 2 cut(s) 1210, 1309
Mph1103I ATGCAT 1 cut(s) 370
MroXI GAANNNNTTC 2 cut(s) 21, 40
MseI TTAA 3 cut(s) 476, 602, 1005
MspA1I CMGCKG 2 cut(s) 446, 887
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 4 cut(s) 440, 449, 905, 1275
NcoI CCATGG 1 cut(s) 926
NdeI CATATG 3 cut(s) 562, 911, 961
NdeII GATC 4 cut(s) 388, 572, 1030, 1331
NlaIII CATG 9 cut(s) 10, 267, 417, 462, 614, 930, 1131, 1255, 1282
NlaIV GGNNCC 2 cut(s) 907, 923
NmuCI GTSAC 4 cut(s) 397, 409, 556, 728
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 3 cut(s) 417, 1131, 1282
PaeI GCATGC 1 cut(s) 1131
PciI ACATGT 1 cut(s) 413
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 2 cut(s) 21, 40
PfeI GAWTC 8 cut(s) 296, 373, 679, 700, 796, 823, 866, 956
PkrI GCNGC 2 cut(s) 445, 507
PscI ACATGT 1 cut(s) 413
PshAI GACNNNNGTC 1 cut(s) 229
Psp1406I AACGTT 1 cut(s) 1078
Psp6I CCWGG 1 cut(s) 217
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 907, 923
PspPI GGNCC 1 cut(s) 1204
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 1 cut(s) 446
PvuII CAGCTG 2 cut(s) 446, 887
RsaI GTAC 4 cut(s) 207, 812, 971, 1301
RsaNI GTAC 4 cut(s) 206, 811, 970, 1300
SalI GTCGAC 1 cut(s) 1163
SaqAI TTAA 3 cut(s) 476, 602, 1005
SatI GCNGC 2 cut(s) 444, 506
Sau3AI GATC 4 cut(s) 388, 572, 1030, 1331
Sau96I GGNCC 1 cut(s) 1204
ScaI AGTACT 1 cut(s) 812
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 4 cut(s) 436, 697, 924, 1068
SfaNI GCATC 4 cut(s) 157, 337, 355, 706
SfcI CTRYAG 1 cut(s) 939
SphI GCATGC 1 cut(s) 1131
Sse9I AATT 9 cut(s) 131, 174, 359, 744, 750, 1000, 1059, 1094, 1118
SsiI CCGC 1 cut(s) 284
SspMI CTAG 3 cut(s) 788, 803, 1274
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 1 cut(s) 926
TaaI ACNGT 2 cut(s) 87, 1262
TaiI ACGT 3 cut(s) 78, 645, 1081
TaqI TCGA 1 cut(s) 1164
TasI AATT 9 cut(s) 131, 174, 359, 744, 750, 1000, 1059, 1094, 1118
TatI WGTACW 2 cut(s) 205, 810
TfiI GAWTC 8 cut(s) 296, 373, 679, 700, 796, 823, 866, 956
Tru1I TTAA 3 cut(s) 476, 602, 1005
Tru9I TTAA 3 cut(s) 476, 602, 1005
TscAI CASTG 5 cut(s) 92, 948, 1040, 1073, 1267
TseFI GTSAC 4 cut(s) 397, 409, 556, 728
TseI GCWGC 2 cut(s) 443, 505
Tsp45I GTSAC 4 cut(s) 397, 409, 556, 728
TspDTI ATGAA 7 cut(s) 23, 53, 447, 774, 948, 1013, 1199
TspGWI ACGGA 3 cut(s) 70, 321, 385
TspRI CASTG 5 cut(s) 92, 948, 1040, 1073, 1267
VneI GTGCAC 1 cut(s) 1064
XapI RAATTY 2 cut(s) 174, 1094
XceI RCATGY 3 cut(s) 417, 1131, 1282
XmiI GTMKAC 1 cut(s) 1164
XmnI GAANNNNTTC 2 cut(s) 21, 40
XspI CTAG 3 cut(s) 788, 803, 1274
ZrmI AGTACT 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.